FastQCFastQC Report
Fri 27 May 2016
SRR939272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388304
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17680.45531336272611145No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13670.3520437595286168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13570.3494684577032428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.2289443322757427No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8160.21014462895051297No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6340.16327413572870741No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5510.14189913057810374No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4140.10661749557048085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC1308.436473E-932.8122863
CTTAGTA600.003990532431.5970212
GTACACG650.0058322129.2380831
CTATACT1802.1464075E-1028.9639344
GCGGTCC350.00836383627.1601854-55
GTACATG22050.026.7187021
TACATGG22200.026.4731792
GAGTACT16050.025.5335112-13
ACATGGG22400.024.3325253
GTACTTT16900.023.9689414-15
GTATCAA40350.022.7256361
GTCTATA1050.002190420422.6247081
GGCTAGC1050.002190420422.6247081
TGCACCG1050.002221866522.56935
AGTACTT16750.022.20364612-13
CATGGGG8800.022.0820064
CATGGGT3401.8189894E-1220.9097924
ACTTTTT19500.020.89725516-17
GTAGCCC1605.0635037E-520.7355443
ACTATAC1150.003766640620.606753
Produced by FastQC (version 0.11.5)