##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939272_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388304 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.921048971939513 33.0 31.0 34.0 30.0 34.0 2 31.988181939923358 33.0 31.0 34.0 30.0 34.0 3 32.03126931476369 34.0 31.0 34.0 30.0 34.0 4 35.53159380279369 37.0 35.0 37.0 33.0 37.0 5 35.58316164654497 37.0 35.0 37.0 33.0 37.0 6 35.68152014916148 37.0 35.0 37.0 33.0 37.0 7 35.60809571881825 37.0 35.0 37.0 33.0 37.0 8 35.59937574683753 37.0 35.0 37.0 33.0 37.0 9 37.2293151757386 39.0 37.0 39.0 34.0 39.0 10-11 37.14780687296552 39.0 37.0 39.0 34.0 39.0 12-13 37.05080169145823 39.0 37.0 39.0 33.0 39.0 14-15 38.276159915942145 40.0 38.0 41.0 33.0 41.0 16-17 38.236339311467304 40.0 38.0 41.0 33.0 41.0 18-19 38.20423302361037 40.0 38.0 41.0 33.0 41.0 20-21 38.11393779100911 40.0 38.0 41.0 33.0 41.0 22-23 38.04592020684824 40.0 38.0 41.0 33.0 41.0 24-25 38.056482807285015 40.0 38.0 41.0 33.0 41.0 26-27 37.976474617825204 40.0 38.0 41.0 33.0 41.0 28-29 37.849203201615225 40.0 38.0 41.0 33.0 41.0 30-31 37.65372620421114 40.0 37.5 41.0 32.5 41.0 32-33 37.448978120235694 40.0 37.0 41.0 32.0 41.0 34-35 37.14908551032181 40.0 36.5 41.0 31.0 41.0 36-37 36.89900052536157 40.0 36.0 41.0 30.5 41.0 38-39 36.59828124356174 39.5 35.5 41.0 30.0 41.0 40-41 36.46685329020561 39.0 35.5 41.0 30.0 41.0 42-43 36.274510435123 39.0 35.0 41.0 30.0 41.0 44-45 36.188115497136266 39.5 35.0 41.0 30.0 41.0 46-47 35.937264359882974 40.0 35.0 41.0 28.5 41.0 48-49 35.68367825209114 39.0 35.0 41.0 27.0 41.0 50-51 34.51672014710124 38.0 34.0 40.0 25.0 40.5 52-53 34.63935859532737 38.5 34.5 39.5 25.0 40.5 54-55 35.14039772961391 39.0 35.0 40.5 26.0 41.0 56-57 35.036320769294164 39.0 35.0 41.0 25.5 41.0 58-59 34.83509183526309 39.0 35.0 41.0 25.0 41.0 60-61 34.546804823025255 38.0 34.0 40.0 24.0 41.0 62-63 34.201017759281385 38.0 34.0 40.0 23.0 41.0 64-65 33.83083486134575 37.0 34.0 40.0 22.0 41.0 66-67 33.4673593370143 37.0 33.5 39.5 21.5 41.0 68-69 33.05735969755655 36.0 33.0 39.0 20.5 41.0 70-71 32.63674595162553 35.5 33.0 39.0 19.5 40.5 72-73 32.19872960360954 35.0 32.0 38.0 18.0 40.0 74-75 31.768875674729077 35.0 32.0 37.0 15.0 39.5 76-77 31.326723391981538 35.0 31.5 37.0 10.0 39.0 78-79 30.906681363055753 35.0 31.0 36.0 7.5 39.0 80-81 30.47195110016894 35.0 31.0 36.0 4.5 37.5 82-83 30.041072201161978 34.0 30.5 35.0 2.0 37.0 84-85 29.626796273023196 34.0 30.0 35.0 2.0 36.5 86-87 29.33915051093988 34.0 30.0 35.0 2.0 36.0 88-89 28.938138674852695 34.0 29.0 35.0 2.0 36.0 90-91 28.54792636697021 34.0 29.0 35.0 2.0 35.5 92-93 28.17002786476575 34.0 29.0 35.0 2.0 35.0 94-95 27.715449750710782 34.0 28.0 35.0 2.0 35.0 96-97 27.170484980839753 34.0 26.5 35.0 2.0 35.0 98-99 26.497159442086613 33.5 25.0 35.0 2.0 35.0 100-101 24.124125685030286 31.0 12.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36063687828482216 1101 2 -0.004944836245833528 1101 3 0.5289785628779313 1101 4 0.4043338656376392 1101 5 0.520857100071936 1101 6 0.5225294128495435 1101 7 0.6077564561945081 1101 8 0.6276905977603064 1101 9 0.41541804899794244 1101 10-11 0.6701288383319977 1101 12-13 0.5666511971811303 1101 14-15 0.625772600753983 1101 16-17 0.7215291481907542 1101 18-19 0.5741213211228313 1101 20-21 0.5622566563842355 1101 22-23 0.5707548576911918 1101 24-25 0.5851616705435774 1101 26-27 0.6307599254443588 1101 28-29 0.5456868390575806 1101 30-31 0.8261948211933969 1101 32-33 0.9637335430934186 1101 34-35 0.924726035897244 1101 36-37 0.9252011149567281 1101 38-39 1.2429191501604393 1101 40-41 1.5935246828942766 1101 42-43 1.3938028195390544 1101 44-45 1.3841585405134325 1101 46-47 1.400382387419988 1101 48-49 1.2860044082071553 1101 50-51 1.3305047897862963 1101 52-53 1.6582280647935264 1101 54-55 1.727193082572846 1101 56-57 1.5770398861253483 1101 58-59 1.5868081934230815 1101 60-61 1.2886525976283068 1101 62-63 1.153309590420875 1101 64-65 0.8402245389522065 1101 66-67 0.9821041440474332 1101 68-69 0.8555134581180894 1101 70-71 1.0574684787846067 1101 72-73 0.9413231622353422 1101 74-75 0.7967006657429003 1101 76-77 0.6109075097310814 1101 78-79 0.8766548551431832 1101 80-81 0.6519715917807503 1101 82-83 0.5632192169053774 1101 84-85 0.5551561931091484 1101 86-87 0.5218210400325347 1101 88-89 0.577409526944141 1101 90-91 0.5595742808472508 1101 92-93 0.912491633639231 1101 94-95 0.4678037904260428 1101 96-97 0.8665066308941043 1101 98-99 0.5738672422559965 1101 100-101 0.367908699531057 1102 1 0.3699676376155878 1102 2 0.16193633063533497 1102 3 0.32740198630135353 1102 4 0.23814982445360044 1102 5 0.28782942954426716 1102 6 0.1458134111335383 1102 7 0.230852766790818 1102 8 0.2419119619816712 1102 9 -0.03972459364469927 1102 10-11 0.26843696789012483 1102 12-13 0.3646992452291826 1102 14-15 0.29912899286037486 1102 16-17 0.18601595642756052 1102 18-19 0.33916314866465314 1102 20-21 0.02176974089732653 1102 22-23 0.12765103958737 1102 24-25 0.33682404720594405 1102 26-27 0.2309181193525518 1102 28-29 0.19678883390957225 1102 30-31 0.40952815452672553 1102 32-33 0.21295597981585956 1102 34-35 0.04472549964670236 1102 36-37 0.08899884125445823 1102 38-39 0.3620606130519022 1102 40-41 0.17658989095948385 1102 42-43 -0.05380550306926324 1102 44-45 -0.09388200002710789 1102 46-47 0.15819823898583962 1102 48-49 0.19726835072109594 1102 50-51 -0.06075700772549908 1102 52-53 0.13046369327915386 1102 54-55 0.042494991624757006 1102 56-57 0.3025868616723244 1102 58-59 0.12221413507901957 1102 60-61 0.07046566069136873 1102 62-63 0.09563584524713065 1102 64-65 0.142857529085191 1102 66-67 0.08726287420616785 1102 68-69 -0.23842422956960263 1102 70-71 -0.16849409092796463 1102 72-73 -0.37064058722840443 1102 74-75 -0.28700804296580884 1102 76-77 -0.4789278613542898 1102 78-79 -0.3230448445565166 1102 80-81 -0.33094882863967 1102 82-83 -0.2900224175862576 1102 84-85 -0.7525711771182237 1102 86-87 -0.5477574670459759 1102 88-89 -0.24501110797649162 1102 90-91 -0.5881895544165836 1102 92-93 -0.2841246254770269 1102 94-95 -0.21036545024319864 1102 96-97 -0.29125827687080275 1102 98-99 -0.5365869619482098 1102 100-101 -0.8979106912883346 1103 1 0.3223485899965368 1103 2 0.5986246423236423 1103 3 0.4069474408468068 1103 4 0.2822515560553285 1103 5 0.3166443646092034 1103 6 0.4184054024755284 1103 7 0.5138614247994724 1103 8 0.5795742996440083 1103 9 0.5682299518098475 1103 10-11 0.5788401064182693 1103 12-13 0.37558939674433134 1103 14-15 0.6330710924274712 1103 16-17 0.8704721252587291 1103 18-19 0.6707378239893274 1103 20-21 0.737475909728488 1103 22-23 0.9587522300635598 1103 24-25 0.9167077052146055 1103 26-27 0.7594624916469215 1103 28-29 1.0065155655113074 1103 30-31 0.8730698211933969 1103 32-33 0.8572335772184516 1103 34-35 0.5038705212917307 1103 36-37 0.811266157271767 1103 38-39 0.7993576909739772 1103 40-41 0.9015628346824229 1103 42-43 0.5897956874069266 1103 44-45 0.6220270908819856 1103 46-47 0.5707388234014275 1103 48-49 0.7864864243141696 1103 50-51 0.03759255937406891 1103 52-53 0.3435995157899754 1103 54-55 0.5817265933563576 1103 56-57 0.513490541326 1103 58-59 0.5996221437370295 1103 60-61 0.8195186909943928 1103 62-63 0.883244070355353 1103 64-65 1.0717671611198227 1103 66-67 0.6443516187949072 1103 68-69 0.7284318310364597 1103 70-71 0.8083727921889192 1103 72-73 0.8702279192650977 1103 74-75 0.8711358964281324 1103 76-77 0.764308069381638 1103 78-79 0.7224097008980301 1103 80-81 0.9398845046936621 1103 82-83 0.6890766083877686 1103 84-85 0.7071149700679271 1103 86-87 0.865391883603376 1103 88-89 0.8914878097724248 1103 90-91 1.1733770040249745 1103 92-93 1.3460160671636636 1103 94-95 1.269477623349875 1103 96-97 0.9515718097092822 1103 98-99 1.140022886536638 1103 100-101 0.2689588974562547 1104 1 0.043777161425108346 1104 2 -0.36949224079323884 1104 3 -0.34188372798436006 1104 4 -0.2330406517368786 1104 5 -0.11252771331287903 1104 6 -0.16394849362836794 1104 7 -0.09402818559013326 1104 8 0.0633405334102477 1104 9 -0.09293887935898226 1104 10-11 0.01730601550917754 1104 12-13 -0.284824564294631 1104 14-15 -0.18307338809201212 1104 16-17 -0.25451975785814795 1104 18-19 -0.5326225656210539 1104 20-21 -0.34037311624553723 1104 22-23 -0.5125870556507266 1104 24-25 -0.539128333746433 1104 26-27 -0.4562842615998335 1104 28-29 -0.4227944994237589 1104 30-31 -0.6409480359494637 1104 32-33 -0.8609725916127147 1104 34-35 -0.9310483098770987 1104 36-37 -0.8218344920788851 1104 38-39 -1.0013322440909533 1104 40-41 -0.9993624899928975 1104 42-43 -0.9511864554502196 1104 44-45 -1.1806677143128184 1104 46-47 -1.0080517610141584 1104 48-49 -1.2567197445169995 1104 50-51 -1.3651022458207436 1104 52-53 -1.3794767829113184 1104 54-55 -1.5399454845657132 1104 56-57 -1.4921750430895884 1104 58-59 -1.5453453887305102 1104 60-61 -1.2716176726419661 1104 62-63 -1.5566260595147767 1104 64-65 -1.3605948518671909 1104 66-67 -1.1053561734128792 1104 68-69 -1.183126610521981 1104 70-71 -1.5508155194993911 1104 72-73 -1.6895691586569797 1104 74-75 -1.4939128048705683 1104 76-77 -1.4911302423066708 1104 78-79 -1.7944734159850881 1104 80-81 -1.5651154953063404 1104 82-83 -1.648415274729114 1104 84-85 -1.8441187961658407 1104 86-87 -1.6752574670459737 1104 88-89 -1.7741777746431602 1104 90-91 -1.8899752687022975 1104 92-93 -1.8885293873817908 1104 94-95 -2.1009606883384357 1104 96-97 -2.3164963721088974 1104 98-99 -2.132241723852971 1104 100-101 -2.127672596050239 1105 1 -0.04709585444790676 1105 2 0.22463474333374478 1105 3 0.40795754185690924 1105 4 0.19172125302502963 1105 5 0.3050913343061694 1105 6 0.24144833176845992 1105 7 0.20009879853684964 1105 8 0.2538167238864375 1105 9 0.30610873968863217 1105 10-11 0.3320282377314001 1105 12-13 0.14803257856251406 1105 14-15 0.29029962778101037 1105 16-17 0.22321833737994012 1105 18-19 0.30047267247418574 1105 20-21 0.404111010738589 1105 22-23 0.5028494522857798 1105 24-25 0.1588478567297571 1105 26-27 0.4345887542731859 1105 28-29 0.5579991513698914 1105 30-31 0.5039725989711741 1105 32-33 0.3252575671174469 1105 34-35 0.19383264250384968 1105 36-37 0.2761020158576244 1105 38-39 0.4784296606709546 1105 40-41 0.43849465286424305 1105 42-43 0.051948465184700865 1105 44-45 0.18250688886178068 1105 46-47 -0.17146445942685773 1105 48-49 -0.2260451413423965 1105 50-51 0.10501680179830686 1105 52-53 0.3694517885172459 1105 54-55 0.2285068963866692 1105 56-57 0.275801147386602 1105 58-59 0.08421810333298652 1105 60-61 0.2572712162469202 1105 62-63 0.061905686516965375 1105 64-65 -0.19821389948624102 1105 66-67 0.13349303293632175 1105 68-69 0.08865910376373165 1105 70-71 -0.07533932902319407 1105 72-73 0.002772111184292214 1105 74-75 0.03521417925641401 1105 76-77 -0.18537627405270385 1105 78-79 0.08667737766570482 1105 80-81 0.198217838026995 1105 82-83 -0.05262162393546177 1105 84-85 -0.13947593902298294 1105 86-87 0.13041713612862793 1105 88-89 0.4190761936108096 1105 90-91 0.3378025090754768 1105 92-93 0.5900817237293232 1105 94-95 0.20312661324886605 1105 96-97 0.3232258501133245 1105 98-99 0.5696630380517895 1105 100-101 0.21974803887039585 1106 1 0.314772832420779 1106 2 0.41026376078094273 1106 3 0.4400052920864752 1106 4 0.5382777268542327 1106 5 0.47134477783234274 1106 6 0.4267559534397165 1106 7 0.42760109422096093 1106 8 0.38191589744015175 1106 9 0.30721066806329134 1106 10-11 0.32664485848438574 1106 12-13 0.3736524132732555 1106 14-15 0.4342505001409762 1106 16-17 0.4327569048730595 1106 18-19 0.4282274934108159 1106 20-21 0.37896179127118756 1106 22-23 0.3122732355732012 1106 24-25 0.5867748539749371 1106 26-27 0.47519940624746937 1106 28-29 0.43329759030469006 1106 30-31 0.28349509667549455 1106 32-33 0.3821560978796086 1106 34-35 0.5230681796939365 1106 36-37 0.38762635010923674 1106 38-39 0.5619696055745322 1106 40-41 0.6695552589248521 1106 42-43 0.881505398150729 1106 44-45 0.7706152451519586 1106 46-47 0.30330597216175903 1106 48-49 0.5032133526888245 1106 50-51 0.34664903320327056 1106 52-53 0.1560564717128372 1106 54-55 0.4032658495547068 1106 56-57 0.5035904035849512 1106 58-59 0.42968412720810534 1106 60-61 0.3682445863111994 1106 62-63 0.5147867700798727 1106 64-65 0.4017861005137675 1106 66-67 0.4788901862880195 1106 68-69 0.7063932635433474 1106 70-71 0.7620573651916729 1106 72-73 0.6556531947471953 1106 74-75 0.7463941608909401 1106 76-77 0.5052688131832923 1106 78-79 0.47860804800546575 1106 80-81 0.25083491791680146 1106 82-83 0.19850326128033302 1106 84-85 0.3761921050541517 1106 86-87 0.47971695247390045 1106 88-89 0.8037639805713219 1106 90-91 0.8282065494795212 1106 92-93 0.39698025449148844 1106 94-95 0.636815777619848 1106 96-97 0.5026220851913763 1106 98-99 0.285640999484297 1106 100-101 -0.18485480777790286 1107 1 0.21213353623309672 1107 2 0.040584564122276845 1107 3 0.07068155619382566 1107 4 -0.0819346609534648 1107 5 -0.08630651515619547 1107 6 -0.06482406966523513 1107 7 -0.11352127314773952 1107 8 -0.017688652457650278 1107 9 0.43944207302196503 1107 10-11 0.19273612303605603 1107 12-13 0.33109709469154325 1107 14-15 0.2930326026913974 1107 16-17 0.3139441438315558 1107 18-19 0.4416017047322498 1107 20-21 0.10030276701098728 1107 22-23 0.35844981070871995 1107 24-25 0.4420467814609381 1107 26-27 0.07562818079648537 1107 28-29 0.3306694022659471 1107 30-31 0.13802277818264486 1107 32-33 0.15352817643643846 1107 34-35 0.19262296508449595 1107 36-37 0.3807615140655187 1107 38-39 0.3838060047569698 1107 40-41 0.1186021797459631 1107 42-43 -0.09316802227049692 1107 44-45 0.12184380642450776 1107 46-47 0.051295397203965365 1107 48-49 -0.1510899442097795 1107 50-51 0.24574260824991967 1107 52-53 0.31340340142047296 1107 54-55 0.27756603617161346 1107 56-57 0.14340867426832915 1107 58-59 0.1826500029745688 1107 60-61 0.11112426284190491 1107 62-63 0.016430776122703605 1107 64-65 -0.006816050023871867 1107 66-67 -0.1258707663468286 1107 68-69 -0.11040003602121118 1107 70-71 0.05611228388002587 1107 72-73 -0.15990710028523836 1107 74-75 -0.09211556984752534 1107 76-77 -0.24232071849715098 1107 78-79 -0.030034091868344603 1107 80-81 0.15493826813452571 1107 82-83 0.23537120760575547 1107 84-85 -0.04875013257136729 1107 86-87 -0.30995024738391663 1107 88-89 -0.2716048099734216 1107 90-91 -0.25610430096036296 1107 92-93 0.2032985696074583 1107 94-95 0.03973055590119756 1107 96-97 0.1256004020846504 1107 98-99 0.08337271547114611 1107 100-101 0.10554552990982558 1108 1 0.18984206258139125 1108 2 0.25145516689045877 1108 3 0.30227143799848477 1108 4 0.3670041076812538 1108 5 0.2677110035838055 1108 6 0.07185158097066591 1108 7 0.29010460068364097 1108 8 0.1567758187515338 1108 9 0.25058219486705013 1108 10-11 0.3146689397911615 1108 12-13 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-1.1600033982058875 2316 96-97 -1.023094310253228 2316 98-99 -1.1595465839413386 2316 100-101 -0.30764314096923684 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1428.0 3 380.0 4 318.0 5 315.0 6 398.0 7 499.0 8 560.0 9 693.0 10 869.0 11 1147.0 12 1506.0 13 2193.0 14 2134.0 15 2364.0 16 3057.0 17 4265.0 18 3496.0 19 2288.0 20 2169.0 21 2234.0 22 2356.0 23 2701.0 24 2988.0 25 3427.0 26 3925.0 27 4622.0 28 5583.0 29 6611.0 30 8119.0 31 9931.0 32 12359.0 33 16123.0 34 22077.0 35 31677.0 36 49866.0 37 80879.0 38 79420.0 39 13324.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80208185388805 19.0468197628928 15.00086507200756 22.150233311211593 2 14.936285289287731 20.897600160865785 40.41134206923932 23.754772480607166 3 19.404847537944708 25.60310621939995 29.825687671289387 25.16635857136596 4 12.559976692560957 16.785223496167422 37.74670692184531 32.908092889426314 5 13.452404894877706 38.16813378919847 33.58344033787651 14.79602097804731 6 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30.010928342036486 21.62110613378971 28-29 21.493103923734022 27.095916218762124 29.911819396640038 21.499160460863816 30-31 21.757164744342234 26.53838571135883 29.80141922564966 21.903030318649275 32-33 21.68025800566159 26.75558911335679 29.657122020546133 21.90703086043548 34-35 22.569537654614162 26.5997016127367 29.272668139674145 21.558092592974994 36-37 22.36811160473152 26.241351951351255 29.6500463899808 21.74049005393643 38-39 21.82681116078868 26.864254498432377 29.658843848945164 21.650090491833772 40-41 21.98284769580492 26.695985740855548 29.64842942756768 21.672737135771854 42-43 21.769832390687053 26.810643075102515 29.089917861876696 22.32960667233374 44-45 21.930691598155462 26.76349325095192 28.970581204996115 22.335233945896498 46-47 22.37670432814067 27.060828621341958 28.475718169507648 22.086748881009726 48-49 22.360640145562815 27.21368166404764 28.316128732114798 22.10954945827475 50-51 21.958080814607104 27.268791915954875 28.555442128522877 22.21768514091514 52-53 22.430883540623487 27.66131481182362 27.744144726215474 22.163656921337424 54-55 21.79523786759623 27.125084465897743 28.369301055193862 22.710376611312164 56-57 22.11951684843193 27.382334819231506 28.10491793114406 22.393230401192508 58-59 22.620408363587988 26.847148442191976 28.162338022966416 22.37010517125362 60-61 22.249921892953324 27.19661544737909 28.695883435883797 21.85757922378379 62-63 22.093149439067368 27.137796679361504 28.593999493584544 22.175054387986584 64-65 21.819380100409003 27.272739020592248 28.32774428657272 22.580136592426033 66-67 21.888961115325518 27.31852246249359 27.910664369779823 22.88185205240107 68-69 21.77947809902935 27.20205244859185 28.319913145752285 22.698556306626514 70-71 22.060359312394986 27.21946490887941 28.11167119038387 22.608504588341734 72-73 22.030019319363948 27.071662563724825 28.32257522598487 22.57574289092636 74-75 21.803223817948496 27.727729150701148 27.90555218834768 22.563494843002676 76-77 22.016290752723737 27.152891195137585 27.931120919559238 22.899697132579437 78-79 21.955819661542435 27.31442966025061 28.04095078155277 22.68879989665418 80-81 22.17807099018611 27.604581795539897 27.775100858150097 22.442246356123896 82-83 22.23697963434061 27.37522916696275 27.781818839559225 22.605972359137418 84-85 21.919245429291298 27.367917824148847 27.80205439627883 22.910782350281025 86-87 21.992503788803745 27.390111771325504 27.795413641760693 22.821970798110055 88-89 22.03739812815533 27.280656528261805 27.568811547909327 23.11313379567354 90-91 21.919631893911 27.147048559500575 27.9750287582882 22.95829078830022 92-93 22.296695613063488 27.108328626689705 27.90964278190426 22.685332978342547 94-95 22.124112260657718 26.900424563965696 27.860830904637897 23.11463227073869 96-97 21.925726021483698 26.875299695102644 28.064127966416052 23.134846316997606 98-99 21.931273342896652 27.028651184429886 27.553858281962807 23.486217190710658 100-101 22.182210972557623 26.349669035039774 28.179634575404837 23.288485416997762 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 298.0 1 177.5 2 75.5 3 91.5 4 78.0 5 74.0 6 128.0 7 271.5 8 442.0 9 537.5 10 531.5 11 515.0 12 565.5 13 630.0 14 670.5 15 690.5 16 738.5 17 791.5 18 832.0 19 877.0 20 911.0 21 982.5 22 1132.0 23 1276.5 24 1448.0 25 1747.0 26 2211.5 27 2760.5 28 3246.0 29 3843.5 30 4558.0 31 5271.5 32 6160.0 33 6910.0 34 7732.5 35 8492.0 36 9266.0 37 10199.5 38 10671.0 39 10807.0 40 10943.5 41 11535.0 42 12090.0 43 12560.0 44 13070.0 45 13497.0 46 13727.5 47 14808.5 48 18269.5 49 18389.0 50 15475.0 51 14055.0 52 13128.5 53 13020.0 54 12629.5 55 11346.0 56 10118.0 57 9028.0 58 7816.5 59 6771.0 60 5828.5 61 4882.5 62 4011.5 63 3119.0 64 2315.0 65 1781.5 66 1351.0 67 1105.0 68 825.0 69 521.5 70 414.0 71 340.0 72 268.0 73 220.5 74 165.5 75 124.5 76 87.0 77 50.0 78 35.0 79 20.5 80 14.0 81 14.5 82 10.0 83 6.5 84 4.5 85 4.5 86 2.5 87 2.0 88 4.0 89 3.0 90 1.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2711792822118752 2 0.10378466356256953 3 0.1125406897688409 4 0.11460093122914006 5 0.1210391857925749 6 0.04970332522971692 7 0.05768676088837611 8 0.0623223041740492 9 0.06026206271375006 10-11 0.055884049610614356 12-13 0.05871688161852569 14-15 0.054982693971733484 16-17 0.05627034488442045 18-19 0.06000453253121266 20-21 0.06451131072561704 22-23 0.07249474638427623 24-25 0.081508302773085 26-27 0.08292471877704068 28-29 0.07558510857472496 30-31 0.07094956528905187 32-33 0.06451131072561704 34-35 0.2286868020932053 36-37 0.3520437595286168 38-39 0.3225565536280852 40-41 0.3049157361242738 42-43 0.32783592237010173 44-45 0.34084119658824014 46-47 0.3471506860604063 48-49 0.3584820140920516 50-51 0.35255881989369153 52-53 0.351914994437348 54-55 0.33826589476286617 56-57 0.3143155877868886 58-59 0.3022116692076311 60-61 0.26139313527545427 62-63 0.3270633318224896 64-65 0.3570655980880959 66-67 0.36144361119123164 68-69 0.37316123449668304 70-71 0.37444888540937 72-73 0.3570655980880959 74-75 0.3611860810086942 76-77 0.34406032386995755 78-79 0.3229428489018913 80-81 0.3220414932630104 82-83 0.3359481231200297 84-85 0.3422576125921958 86-87 0.33697824385017927 88-89 0.3501122831595863 90-91 0.37573653632205695 92-93 0.37071469776257776 94-95 0.3699421072149656 96-97 0.3740625901355639 98-99 0.3628600271951873 100-101 0.34238637768346447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 388304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.82560815205495 #Duplication Level Percentage of deduplicated Percentage of total 1 85.76619036930012 58.171440206826986 2 8.752752041779086 11.873214604756143 3 2.2325206067265375 4.542662035896663 4 0.9187285049796698 2.4925327830749735 5 0.5254485117419065 1.78194324307435 6 0.35361897945509835 1.439065340139064 7 0.2486230081675066 1.1804104710688121 8 0.17597418331360232 0.9548444801845022 9 0.13773765186990536 0.8407926003161124 >10 0.8112437405490799 9.937735993376398 >50 0.05435578080460137 2.463395189817309 >100 0.020145848826377925 2.418793685807621 >500 0.0015204414208587114 0.7450771426118126 >1k 0.0011403310656440335 1.1580922230492257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1768 0.45531336272611145 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1367 0.3520437595286168 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1357 0.3494684577032428 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 889 0.2289443322757427 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 816 0.21014462895051297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 634 0.16327413572870741 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 551 0.14189913057810374 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 414 0.10661749557048085 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2876509126869668E-4 0.0 12-13 0.0 0.0 0.0 2.5753018253739336E-4 0.0 14-15 0.0 0.0 0.0 2.5753018253739336E-4 0.0 16-17 0.0 0.0 0.0 2.5753018253739336E-4 0.0 18-19 0.0 0.0 0.0 3.8629527380609004E-4 0.0 20-21 0.0 0.0 0.0 5.150603650747867E-4 2.5753018253739336E-4 22-23 0.0 0.0 0.0 6.438254563434834E-4 2.5753018253739336E-4 24-25 0.0 0.0 0.0 9.013556388808768E-4 2.5753018253739336E-4 26-27 0.0 0.0 0.0 0.0019314763690304504 2.5753018253739336E-4 28-29 0.0 0.0 0.0 0.004120482920598295 2.5753018253739336E-4 30-31 0.0 0.0 0.0 0.012361448761794883 2.5753018253739336E-4 32-33 0.0 0.0 0.0 0.027813259714038488 2.5753018253739336E-4 34-35 0.0 0.0 0.0 0.04841567431702995 2.5753018253739336E-4 36-37 0.0 0.0 0.0 0.07906176603897977 2.5753018253739336E-4 38-39 0.0 0.0 0.0 0.12657608471712883 2.5753018253739336E-4 40-41 0.0 0.0 0.0 0.1825888994190119 2.5753018253739336E-4 42-43 0.0 0.0 0.0 0.24207837158514978 2.5753018253739336E-4 44-45 0.0 0.0 0.0 0.32062507725905476 2.5753018253739336E-4 46-47 0.0 0.0 0.0 0.4054812724051259 2.5753018253739336E-4 48-49 0.0 0.0 0.0 0.5016687955828423 2.5753018253739336E-4 50-51 0.0 0.0 0.0 0.6098314722485475 2.5753018253739336E-4 52-53 0.0 0.0 0.0 0.723402282747538 2.5753018253739336E-4 54-55 0.0 0.0 0.0 0.8428962874448886 2.5753018253739336E-4 56-57 0.0 0.0 0.0 0.9904610820388149 2.5753018253739336E-4 58-59 0.0 0.0 0.0 1.1408587086406525 2.5753018253739336E-4 60-61 0.0 0.0 0.0 1.335551526638922 2.5753018253739336E-4 62-63 0.0 0.0 0.0 1.5332059417363715 2.5753018253739336E-4 64-65 0.0 0.0 0.0 1.7412903292265853 2.5753018253739336E-4 66-67 0.0 0.0 0.0 1.9774455066133751 2.5753018253739336E-4 68-69 0.0 0.0 0.0 2.238581111706292 2.5753018253739336E-4 70-71 0.0 0.0 0.0 2.528173801969591 2.5753018253739336E-4 72-73 0.0 0.0 0.0 2.833733363550208 2.5753018253739336E-4 74-75 0.0 0.0 0.0 3.1663335942972517 2.5753018253739336E-4 76-77 0.0 0.0 0.0 3.525073138571841 2.5753018253739336E-4 78-79 0.0 0.0 0.0 3.9207682640405457 2.5753018253739336E-4 80-81 0.0 0.0 0.0 4.362561292183444 2.5753018253739336E-4 82-83 0.0 0.0 0.0 4.8187760105484365 2.5753018253739336E-4 84-85 0.0 0.0 0.0 5.271127776175367 2.5753018253739336E-4 86-87 0.0 0.0 0.0 5.762624129547983 2.5753018253739336E-4 88-89 0.0 0.0 0.0 6.261460093122914 2.5753018253739336E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTATAC 130 8.436473E-9 32.812286 3 CTTAGTA 60 0.0039905324 31.597021 2 GTACACG 65 0.00583221 29.238083 1 CTATACT 180 2.1464075E-10 28.963934 4 GCGGTCC 35 0.008363836 27.16018 54-55 GTACATG 2205 0.0 26.718702 1 TACATGG 2220 0.0 26.473179 2 GAGTACT 1605 0.0 25.53351 12-13 ACATGGG 2240 0.0 24.332525 3 GTACTTT 1690 0.0 23.96894 14-15 GTATCAA 4035 0.0 22.725636 1 GTCTATA 105 0.0021904204 22.624708 1 GGCTAGC 105 0.0021904204 22.624708 1 TGCACCG 105 0.0022218665 22.5693 5 AGTACTT 1675 0.0 22.203646 12-13 CATGGGG 880 0.0 22.082006 4 CATGGGT 340 1.8189894E-12 20.909792 4 ACTTTTT 1950 0.0 20.897255 16-17 GTAGCCC 160 5.0635037E-5 20.735544 3 ACTATAC 115 0.0037666406 20.60675 3 >>END_MODULE