FastQCFastQC Report
Fri 27 May 2016
SRR939272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388304
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17650.4545407721784993No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15170.39067328690922576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.3729037043141456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10020.25804524290246816No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7220.185936791791998No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5490.14138407021302896No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4760.12258436688779926No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4570.11769129341958878No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG550.002546156534.6318051
GTATGGT902.368103E-531.7458231
CGCACTG804.510804E-429.662075
TAAGGTG1205.405549E-627.6845975
GTACTAT700.00828332727.2107051
ACTGCGG908.981662E-426.3662838
GTAAGGT1151.2870846E-424.761384
TATACCA1551.4171565E-624.4951295
GGTAAGG1000.001659940323.7296543
CAACCGT1201.7179281E-423.7296546
TAGCATG1201.7179281E-423.7296544
TACTAGA1000.001659940323.7296542
GTACATG22750.023.0244431
AACTGCG1050.00220457422.5996727
GAGTACT16400.021.8514612-13
TACATGG23000.021.6662062
ACATGGG22650.020.7438053
AGTACTT17050.020.60082812-13
GTACTTT17600.020.22674214-15
CATGGGA12450.019.8223634
Produced by FastQC (version 0.11.5)