##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939272_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 388304 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28140323045861 33.0 31.0 34.0 31.0 34.0 2 32.47689181672092 34.0 31.0 34.0 31.0 34.0 3 32.55752194157155 34.0 31.0 34.0 31.0 34.0 4 36.091721949812516 37.0 35.0 37.0 35.0 37.0 5 36.03233806502122 37.0 35.0 37.0 35.0 37.0 6 36.07888664551486 37.0 35.0 37.0 35.0 37.0 7 36.02745529276031 37.0 35.0 37.0 35.0 37.0 8 36.00967025835428 37.0 35.0 37.0 35.0 37.0 9 37.71073694837035 39.0 37.0 39.0 35.0 39.0 10-11 37.63058711731015 39.0 37.0 39.0 35.0 39.0 12-13 37.546736577526886 39.0 37.0 39.0 35.0 39.0 14-15 38.82428844410565 40.0 38.0 41.0 35.0 41.0 16-17 38.77351765626932 40.0 38.0 41.0 34.5 41.0 18-19 38.91610439243479 40.0 38.0 41.0 35.0 41.0 20-21 38.92955004326507 40.0 38.0 41.0 35.0 41.0 22-23 38.87279940459022 40.0 38.0 41.0 35.0 41.0 24-25 38.803060231159094 40.0 38.0 41.0 34.0 41.0 26-27 38.624716716799206 40.0 38.0 41.0 34.0 41.0 28-29 38.483315907124314 40.0 38.0 41.0 34.0 41.0 30-31 38.286025897235156 40.0 38.0 41.0 34.0 41.0 32-33 38.07883513947834 40.0 37.0 41.0 33.5 41.0 34-35 38.00811091309902 40.0 37.0 41.0 33.0 41.0 36-37 37.902102476410235 40.0 37.0 41.0 33.0 41.0 38-39 37.79635285755491 40.0 37.0 41.0 33.0 41.0 40-41 37.61648862746714 40.0 37.0 41.0 33.0 41.0 42-43 37.41341577732911 40.0 37.0 41.0 32.5 41.0 44-45 37.144106164242444 40.0 36.5 41.0 31.5 41.0 46-47 36.78118561745437 40.0 36.0 41.0 31.0 41.0 48-49 36.42284009435906 40.0 36.0 41.0 30.0 41.0 50-51 36.15965583666406 39.0 35.5 41.0 29.5 41.0 52-53 35.932131783345014 39.0 35.0 41.0 29.0 41.0 54-55 35.659330060983145 39.0 35.0 40.5 28.0 41.0 56-57 35.4191496868433 38.5 35.0 40.0 28.0 41.0 58-59 35.12758174007993 38.0 34.5 40.0 27.0 41.0 60-61 34.78294583625201 38.0 34.0 40.0 26.0 41.0 62-63 34.410355031109646 37.5 34.0 40.0 26.0 41.0 64-65 33.992325600560385 37.0 33.5 39.5 25.5 41.0 66-67 33.60622476204211 36.0 33.0 39.0 25.0 40.5 68-69 33.21416853805266 36.0 33.0 39.0 23.5 40.0 70-71 32.80800867361655 35.0 32.5 38.5 23.0 40.0 72-73 32.31134497713132 35.0 32.0 37.5 21.5 39.5 74-75 31.82091093576167 35.0 31.5 37.0 20.0 39.0 76-77 29.838403673410525 33.0 29.0 35.0 18.0 37.5 78-79 30.815550445012157 34.0 31.0 36.0 18.0 38.0 80-81 30.85118232106803 34.0 31.0 36.0 18.0 37.0 82-83 30.544031222959333 34.0 31.0 35.0 16.0 37.0 84-85 30.185886573406403 34.0 31.0 35.0 10.0 36.5 86-87 29.691966861016112 34.0 30.0 35.0 4.5 36.0 88-89 29.201700214265113 34.0 29.0 35.0 2.0 36.0 90-91 28.796293625612922 34.0 29.0 35.0 2.0 35.0 92-93 28.471002101446288 34.0 29.0 35.0 2.0 35.0 94-95 28.07546793234167 34.0 29.0 35.0 2.0 35.0 96-97 27.541485537104947 33.0 27.5 35.0 2.0 35.0 98-99 26.81321464625654 33.0 26.5 35.0 2.0 35.0 100-101 23.460017151510158 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22162874808879707 1101 2 0.221674939870681 1101 3 0.26449077954549693 1101 4 0.17506567955926045 1101 5 0.3173647125932533 1101 6 0.20163430933255455 1101 7 0.2838958849065705 1101 8 0.2752113329704855 1101 9 0.3364988930738164 1101 10-11 0.42262840280225333 1101 12-13 0.30825553084117274 1101 14-15 0.26107383815548957 1101 16-17 0.3032677799881398 1101 18-19 0.44040493571935 1101 20-21 0.39647932906134997 1101 22-23 0.5524984508865174 1101 24-25 0.5812496729231711 1101 26-27 0.6684830748064741 1101 28-29 0.5852872748167428 1101 30-31 0.5193901506197207 1101 32-33 0.38936649520217514 1101 34-35 0.3617683334074826 1101 36-37 0.4072431468521742 1101 38-39 0.4710202286251288 1101 40-41 0.37526892840632087 1101 42-43 0.229390348453812 1101 44-45 0.4093974220158998 1101 46-47 0.3971170426169621 1101 48-49 0.09960685867824992 1101 50-51 0.010817049595615913 1101 52-53 0.2827891128082527 1101 54-55 0.31428798161960714 1101 56-57 0.28900097554985393 1101 58-59 0.26177160389507037 1101 60-61 0.05685492359083355 1101 62-63 0.23814276601522266 1101 64-65 0.5767014548108804 1101 66-67 0.3707622691955095 1101 68-69 0.3425530089130646 1101 70-71 0.3166540661828563 1101 72-73 0.2996767225274155 1101 74-75 0.33106349722029904 1101 76-77 0.6262241221346407 1101 78-79 0.5778451513061569 1101 80-81 0.7099944059131715 1101 82-83 0.5438257464099969 1101 84-85 0.4187950877537254 1101 86-87 0.6756782409566142 1101 88-89 0.770824663337148 1101 90-91 0.7255940079401064 1101 92-93 0.7843922390379205 1101 94-95 0.881313331260305 1101 96-97 0.672567723395801 1101 98-99 0.7960343914716823 1101 100-101 0.336038944014561 1102 1 -0.11934615538610416 1102 2 0.04870732940307221 1102 3 0.07851909357381714 1102 4 0.023816215809794983 1102 5 -0.08769212996358533 1102 6 0.11872997642822014 1102 7 -0.04922294821226103 1102 8 0.043363413622564906 1102 9 0.06807868715360854 1102 10-11 0.04849980992365488 1102 12-13 -0.14297248288684017 1102 14-15 0.04900015733180396 1102 16-17 0.01957784004820695 1102 18-19 0.2629060082204191 1102 20-21 0.048251637083659205 1102 22-23 0.19295587259394154 1102 24-25 0.09417331084680569 1102 26-27 0.3041008354242294 1102 28-29 0.03544278747225604 1102 30-31 0.0434141746437362 1102 32-33 0.06785748619316934 1102 34-35 0.2497321901213425 1102 36-37 0.15889211725114905 1102 38-39 0.2809864448413464 1102 40-41 0.4855890699764629 1102 42-43 0.16552290958637172 1102 44-45 0.3209160835345628 1102 46-47 0.3524339666838827 1102 48-49 -0.01229522197383659 1102 50-51 0.20569049446906007 1102 52-53 0.5249196361887769 1102 54-55 0.12231028964190926 1102 56-57 0.07555985585873515 1102 58-59 0.08726227313574952 1102 60-61 0.05394576443167409 1102 62-63 0.04481104143349768 1102 64-65 0.22627513563455892 1102 66-67 0.03768365486689618 1102 68-69 -0.1519504230903621 1102 70-71 0.0624847182635051 1102 72-73 -0.010772119171427619 1102 74-75 -0.016976507069710323 1102 76-77 -0.08776665435613396 1102 78-79 -0.21451864105763718 1102 80-81 -0.013675692756926594 1102 82-83 0.14740253748679066 1102 84-85 -0.15792895772031912 1102 86-87 0.20084179737017038 1102 88-89 0.4885155685280509 1102 90-91 0.5840506788967765 1102 92-93 0.3740935474892275 1102 94-95 0.5497764372234109 1102 96-97 0.4620893879174659 1102 98-99 0.47805122973851866 1102 100-101 0.6278664858421017 1103 1 0.2161516801117287 1103 2 0.1381283250740708 1103 3 0.2055483143530381 1103 4 0.1858833153768913 1103 5 0.28338470986325603 1103 6 0.08680356950181789 1103 7 0.22174566650635796 1103 8 0.2533201235792788 1103 9 0.12570855728348107 1103 10-11 0.19162751555136737 1103 12-13 0.3138051145157519 1103 14-15 0.2862837071153521 1103 16-17 0.2861498097451758 1103 18-19 0.1983091467485636 1103 20-21 0.4749967669537867 1103 22-23 0.4309834699965336 1103 24-25 0.3984887870372873 1103 26-27 0.6062518094502067 1103 28-29 0.7228751467796144 1103 30-31 0.7612172049467674 1103 32-33 0.8533688498295291 1103 34-35 0.851125588389742 1103 36-37 0.7538596497186774 1103 38-39 0.47558871756861976 1103 40-41 0.7642687236561159 1103 42-43 1.0123167407552032 1103 44-45 1.1481617545302285 1103 46-47 1.206059507809428 1103 48-49 1.241289734736121 1103 50-51 1.20738151178508 1103 52-53 0.7783016708208095 1103 54-55 0.8554001164817393 1103 56-57 0.9465690549929349 1103 58-59 0.8322568618803388 1103 60-61 0.80489273412865 1103 62-63 0.8406714310438872 1103 64-65 0.8763021919116198 1103 66-67 0.5967339795422149 1103 68-69 0.7460474124074707 1103 70-71 1.0310317961855802 1103 72-73 0.8835731189238132 1103 74-75 0.8472686228004207 1103 76-77 0.8936321551676762 1103 78-79 0.8610360126220158 1103 80-81 1.1191706275893942 1103 82-83 1.3584414985257531 1103 84-85 1.48479290375154 1103 86-87 1.5006253471537185 1103 88-89 1.3430204386579199 1103 90-91 1.5626085793296802 1103 92-93 1.7930599977056794 1103 94-95 1.986870593067568 1103 96-97 1.641134301337381 1103 98-99 1.6890225500848395 1103 100-101 2.023564537790154 1104 1 0.14482051128056384 1104 2 -0.09831648012073657 1104 3 -0.15290947785475595 1104 4 -0.01535045085687159 1104 5 0.01385548908402967 1104 6 -0.24079383309558722 1104 7 -0.0139848529741613 1104 8 -0.133660395901245 1104 9 -0.023707027132104486 1104 10-11 0.05641647659032145 1104 12-13 -0.145472482886845 1104 14-15 -0.168797461715819 1104 16-17 -0.20476739804703215 1104 18-19 -0.07804637273196136 1104 20-21 0.003073065655087248 1104 22-23 -0.12150841312035254 1104 24-25 -0.3332076415341376 1104 26-27 -0.23661345029005787 1104 28-29 0.008418977948444706 1104 30-31 0.2015094127389787 1104 32-33 0.07607177190745773 1104 34-35 -0.2338987622596136 1104 36-37 -0.1166435970345745 1104 38-39 0.11437930198420077 1104 40-41 0.09207716521455467 1104 42-43 0.216237195300657 1104 44-45 0.3585351311536087 1104 46-47 0.22308872858864248 1104 48-49 0.16228811135950139 1104 50-51 0.4466428754214391 1104 52-53 0.25093154095068115 1104 54-55 0.33850076583238575 1104 56-57 0.5016908082396867 1104 58-59 0.15482179694527076 1104 60-61 -0.11039947366356273 1104 62-63 0.06356104143349484 1104 64-65 -0.06580819769877166 1104 66-67 0.21786222629546614 1104 68-69 0.058644815004875284 1104 70-71 -0.21555099602220906 1104 72-73 -0.16106973821904802 1104 74-75 0.009630635787431174 1104 76-77 0.049257155167676814 1104 78-79 0.056433739894742985 1104 80-81 0.05465764057640499 1104 82-83 -0.11081174822749418 1104 84-85 -0.007155148196510197 1104 86-87 -0.052670107391737275 1104 88-89 0.0017298542423382912 1104 90-91 -0.12963979729369868 1104 92-93 0.09903402367970315 1104 94-95 -0.06843784849087342 1104 96-97 -0.5376725168444381 1104 98-99 -0.13236543692814706 1104 100-101 0.3850093429849579 1105 1 0.021328447788498295 1105 2 -0.028475210279466978 1105 3 0.05510639516111837 1105 4 0.04068129517487051 1105 5 0.12877612400466631 1105 6 0.12190457960282686 1105 7 0.20514213115281876 1105 8 0.16935547711462817 1105 9 0.21510249667742443 1105 10-11 0.20633711151095468 1105 12-13 0.05821799330363575 1105 14-15 0.2532660303476746 1105 16-17 0.19527228449265266 1105 18-19 0.13691394472835583 1105 20-21 0.25886671644873616 1105 22-23 0.2267852376733046 1105 24-25 0.24258600925950446 1105 26-27 0.3132278195512157 1105 28-29 0.267982470011944 1105 30-31 0.4211919524215162 1105 32-33 0.1567463750820579 1105 34-35 0.16649806313721172 1105 36-37 0.2266500537590801 1105 38-39 0.41918088928579067 1105 40-41 0.42418033981773107 1105 42-43 0.42762608418954784 1105 44-45 0.24393195655043343 1105 46-47 0.17594587144579066 1105 48-49 0.03185160342299298 1105 50-51 0.2713650976436597 1105 52-53 0.4180743980935304 1105 54-55 0.20445314678476478 1105 56-57 0.058595570144447606 1105 58-59 0.1583932255166971 1105 60-61 -0.007859791123877358 1105 62-63 2.6739063984138056E-4 1105 64-65 0.17478704039646686 1105 66-67 0.13679079772403213 1105 68-69 0.2262241800842375 1105 70-71 0.4107982103269947 1105 72-73 0.3599620078127046 1105 74-75 0.5200274611842559 1105 76-77 0.2578682662787877 1105 78-79 0.3983781843391867 1105 80-81 0.585332243751008 1105 82-83 0.6249819025661552 1105 84-85 0.4409797724384106 1105 86-87 0.2884410037193774 1105 88-89 0.36192826694074753 1105 90-91 0.8352411550872567 1105 92-93 0.3601054522511298 1105 94-95 0.2005700880170629 1105 96-97 0.23788303871111793 1105 98-99 0.47219805513534396 1105 100-101 0.4651680731436869 1106 1 0.1316131126186164 1106 2 0.06417860055616131 1106 3 0.08855451270014214 1106 4 0.0946298718507208 1106 5 0.017114048706226015 1106 6 0.015522577766276413 1106 7 -0.014394140656179388 1106 8 -0.1352818047953761 1106 9 -0.051633040512662376 1106 10-11 0.034000105083045185 1106 12-13 0.15914774536975074 1106 14-15 0.12865629664703704 1106 16-17 0.10161985106748972 1106 18-19 0.04261871975131015 1106 20-21 -0.03890646996081415 1106 22-23 0.30163601820589747 1106 24-25 0.1388899578361844 1106 26-27 0.33287887556590334 1106 28-29 0.23135482997520995 1106 30-31 0.29134805894126004 1106 32-33 0.28944839528407584 1106 34-35 0.49140623577265785 1106 36-37 0.45831901610986137 1106 38-39 0.44067997101214473 1106 40-41 0.4583975115349048 1106 42-43 0.7098460107276523 1106 44-45 0.7970081732630163 1106 46-47 0.9642378824650706 1106 48-49 0.6533506851493485 1106 50-51 0.6438054098567036 1106 52-53 0.8587080069089623 1106 54-55 0.9133374443054265 1106 56-57 0.8298765618799848 1106 58-59 1.0665888178031935 1106 60-61 0.9238665633297458 1106 62-63 1.170389061897879 1106 64-65 1.1788159660163018 1106 66-67 1.3001172440050226 1106 68-69 1.093154850424007 1106 70-71 0.9213124435685032 1106 72-73 0.7539243585932311 1106 74-75 0.8850182784478022 1106 76-77 0.7859789182530754 1106 78-79 0.8083070181316572 1106 80-81 0.9289158066527534 1106 82-83 1.0369883304816732 1106 84-85 0.860137256368624 1106 86-87 0.6179635014236915 1106 88-89 0.754134419374175 1106 90-91 1.0187466647291252 1106 92-93 1.011379556934326 1106 94-95 1.1527624663458056 1106 96-97 0.8772310644227801 1106 98-99 0.9684216547680329 1106 100-101 0.5179688077626068 1107 1 0.15609547287809988 1107 2 0.11543159975637707 1107 3 0.12652216577043163 1107 4 0.09802896542576889 1107 5 0.16874028171076105 1107 6 0.023875905767702932 1107 7 -0.0012198760156394428 1107 8 0.1980293122400809 1107 9 0.020747657967746136 1107 10-11 0.06054858104500482 1107 12-13 0.07716960620685853 1107 14-15 0.19430545687097123 1107 16-17 0.1563385927363754 1107 18-19 0.04977236766742266 1107 20-21 0.0885262146566248 1107 22-23 -0.11143160820483189 1107 24-25 -0.18098029898421686 1107 26-27 0.06466151130748443 1107 28-29 -0.008047995937886299 1107 30-31 -0.014381524280992153 1107 32-33 0.2150349055480021 1107 34-35 0.17796759718739708 1107 36-37 0.055637508956216664 1107 38-39 -0.06948398526618149 1107 40-41 -0.021563387780837218 1107 42-43 -0.20198861115095923 1107 44-45 -0.06771678896927824 1107 46-47 0.04718243058557903 1107 48-49 0.15125124500005427 1107 50-51 -0.10421733963232072 1107 52-53 -0.31095785997097636 1107 54-55 -0.20468663816146915 1107 56-57 -0.15538292447920554 1107 58-59 -0.12601537663383766 1107 60-61 -0.2864440205145584 1107 62-63 0.12531219350722722 1107 64-65 0.1932010188910951 1107 66-67 -0.042913503351229565 1107 68-69 0.01058797936739353 1107 70-71 0.035170970183635575 1107 72-73 -0.2447870961299472 1107 74-75 0.010529253298955155 1107 76-77 0.2829130691461721 1107 78-79 0.35326169688399034 1107 80-81 0.0890508817438409 1107 82-83 0.24254462658049292 1107 84-85 -0.05601843544689444 1107 86-87 -0.019444300940119064 1107 88-89 -0.08838535313093487 1107 90-91 0.10526803680768282 1107 92-93 0.3201860974124209 1107 94-95 0.47229947869806566 1107 96-97 0.5882414616501848 1107 98-99 0.5179417827339101 1107 100-101 0.32717524160246825 1108 1 0.09597306629734703 1108 2 -0.003613011265834132 1108 3 0.24506142852345647 1108 4 0.05061747156013752 1108 5 0.16770852899450972 1108 6 0.16177983344675084 1108 7 0.1808528941351213 1108 8 0.06107291256574854 1108 9 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60-61 -0.5174245104824138 2316 62-63 -0.010886675797728174 2316 64-65 -0.047678595342368624 2316 66-67 -0.4316370373275049 2316 68-69 -0.32086917615860244 2316 70-71 -0.2891445159043897 2316 72-73 -0.494648530560724 2316 74-75 -0.252166124153657 2316 76-77 -0.31600057679108673 2316 78-79 -0.3026384250537113 2316 80-81 -0.3307473667873637 2316 82-83 -0.24079702068699405 2316 84-85 -0.751617592968234 2316 86-87 -0.6932886640927656 2316 88-89 -0.8254571855220227 2316 90-91 -1.123822418795907 2316 92-93 -0.6621883621818547 2316 94-95 -0.5759047115247462 2316 96-97 -0.707878702411449 2316 98-99 -0.3469457020238771 2316 100-101 0.8485586802456382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 11.0 6 17.0 7 50.0 8 102.0 9 216.0 10 356.0 11 584.0 12 826.0 13 1249.0 14 1550.0 15 2041.0 16 2907.0 17 4088.0 18 3839.0 19 2374.0 20 2065.0 21 2138.0 22 2207.0 23 2461.0 24 2752.0 25 3278.0 26 3867.0 27 4700.0 28 5969.0 29 7164.0 30 8715.0 31 10805.0 32 13180.0 33 16923.0 34 23035.0 35 33510.0 36 52530.0 37 88437.0 38 77523.0 39 6829.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00934497388107 19.675314447138536 15.419077793873479 21.896262785106916 2 15.527524825909596 21.347449421072152 39.47087848695867 23.65414726605958 3 19.55812971280234 25.57042935432033 30.115064485557706 24.756376447319624 4 13.030769706209568 17.30087766286209 37.2865074786765 32.38184515225184 5 13.501534879887922 38.38384358646834 33.43334088755202 14.681280646091722 6 30.849540566154353 36.70474679632453 17.40028431332152 15.045428324199598 7 28.016708558243025 33.76246446083481 20.506355844905023 17.71447113601714 8 26.490069636161355 34.22318595739421 19.69539330009477 19.591351106349663 9 26.533334706827638 17.495570480860355 20.74354114302196 35.22755366929004 10-11 25.088976678066672 26.97796056697845 26.69158700399687 21.24147575095801 12-13 26.30928344802011 24.513396720095596 27.531779224525117 21.64554060735918 14-15 23.002237977630525 25.615186313569254 26.831652060149835 24.55092364865039 16-17 21.690378788756735 29.093093417906353 27.20063043469863 22.01589735863828 18-19 21.598155053772302 27.372110511351934 29.940459021797356 21.08927541307841 20-21 22.691628208826074 26.142532654827143 29.73546499649759 21.43037413984919 22-23 22.15467777823561 26.02574271704644 29.932604351229962 21.886975153487988 24-25 22.43055036917047 26.408886874425384 29.731292315537093 21.429270440867054 26-27 21.947764816772533 26.322844490685714 30.200630180413928 21.528760512127818 28-29 21.38401870699246 27.077624747620423 30.17712925954922 21.3612272858379 30-31 21.775593349540568 26.5709341134781 29.901314434051674 21.752158102929663 32-33 21.6063960196135 26.74463822159957 29.770231571140137 21.878734187646792 34-35 22.48615419481862 26.66403979453739 29.457022955514432 21.392783055129545 36-37 22.308555208248816 26.27055667389185 29.78668325496393 21.634204862895405 38-39 21.775663024512106 26.855406249396374 29.85120178223049 21.517728943861027 40-41 21.926652293854467 26.691316812326622 29.81276707544181 21.569263818377106 42-43 21.87499597587845 26.79408215904101 29.009344976029112 22.32157688905143 44-45 21.99824105752875 26.72163254584389 29.039934174861543 22.24019222176582 46-47 22.377312621039184 27.091402612386172 28.529451151674976 22.001833614899667 48-49 22.484831472248548 27.08161646544975 28.411502328072853 22.02204973422885 50-51 22.023852445506613 27.45297498866867 28.439701883060696 22.08347068276402 52-53 22.452511434340106 27.59139746178252 27.847768758498496 22.108322345378877 54-55 21.752673163294737 27.183212122460752 28.302695825950803 22.761418888293708 56-57 22.150042234949936 27.459284478140837 28.055466850714904 22.335206436194323 58-59 22.62684714474816 27.037975596314208 28.020072005809908 22.31510525312772 60-61 22.22262283091852 27.245955425987255 28.70565905567374 21.825762687420486 62-63 22.06133635847402 27.150680592271726 28.520363686465284 22.267619362788967 64-65 21.710438213358604 27.35446969384812 28.305013597593636 22.630078495199637 66-67 21.833537640611482 27.37790494045902 27.791112118340273 22.997445300589227 68-69 21.716646901199706 27.209901533207915 28.31058695067132 22.76286461492106 70-71 22.02526886151057 27.284421484197946 28.041946516131688 22.64836313815979 72-73 22.03389612262557 27.044918414438175 28.284539948081918 22.636645514854344 74-75 21.586566195558117 27.781841031768924 27.737030780007416 22.89456199266554 76-77 21.70753426425419 27.067083085976822 28.033322148517154 23.192060501251838 78-79 21.71803535374346 27.157201574024477 28.108646833408873 23.01611623882319 80-81 21.876098537086612 27.55095602503731 27.838233533049788 22.734711904826288 82-83 21.883240965841196 27.277854464543243 27.89837343936709 22.940531130248466 84-85 21.582832007911325 27.32845914541184 27.801928386006843 23.28678046066999 86-87 21.551491903415865 27.302689666817475 27.867897362922633 23.277921066844026 88-89 21.4982699051355 27.122305690577896 27.687978231039374 23.69144617324723 90-91 21.340484860074895 27.036078944172093 28.002723816858204 23.62071237889481 92-93 21.724216078124357 26.988004244097407 27.93738926202151 23.350390415756728 94-95 21.350153487988795 26.768330998393015 27.964429931187933 23.91708558243026 96-97 21.040473443487574 26.612396472866617 28.277586633153405 24.069543450492397 98-99 21.082064567967365 26.818163088713998 27.604660266183195 24.49511207713544 100-101 20.965714918504364 26.169511953294204 28.497719564360825 24.36705356384061 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 112.0 1 78.0 2 49.5 3 55.5 4 56.0 5 66.5 6 116.0 7 260.5 8 421.5 9 504.5 10 490.5 11 465.0 12 495.5 13 523.0 14 576.5 15 635.0 16 667.5 17 694.5 18 738.0 19 801.5 20 858.5 21 961.0 22 1140.0 23 1279.0 24 1427.0 25 1756.0 26 2193.5 27 2705.0 28 3175.5 29 3785.5 30 4481.0 31 5201.5 32 6245.0 33 6975.0 34 7696.0 35 8508.0 36 9194.5 37 10161.0 38 10761.0 39 10915.0 40 11152.0 41 11670.0 42 12197.0 43 12733.0 44 13208.5 45 13660.0 46 13870.0 47 14986.0 48 18439.5 49 18565.5 50 15720.5 51 14212.5 52 13202.0 53 12989.5 54 12657.0 55 11456.5 56 10106.0 57 8908.5 58 7741.0 59 6697.0 60 5749.5 61 4846.0 62 4013.5 63 3139.0 64 2325.0 65 1794.5 66 1316.0 67 1040.5 68 761.0 69 467.0 70 370.5 71 292.0 72 224.0 73 184.0 74 141.0 75 120.5 76 84.0 77 36.5 78 23.5 79 14.5 80 7.5 81 5.5 82 1.5 83 0.5 84 1.5 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2670587992912769 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0018027112777617535 16-17 1.287650912686967E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.575301825373934E-4 26-27 6.438254563434835E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002446536734105237 36-37 0.02021611932918538 38-39 0.006824549837240924 40-41 0.01828464296015493 42-43 0.005279368742016565 44-45 6.438254563434835E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.150603650747868E-4 60-61 0.0015451810952243602 62-63 6.438254563434835E-4 64-65 0.0 66-67 0.0 68-69 1.287650912686967E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2250813795376818 78-79 0.0 80-81 9.013556388808768E-4 82-83 0.0 84-85 0.0 86-87 0.1013381268284643 88-89 0.3427726729572706 90-91 0.3468931558778689 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 2.575301825373934E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 388304.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.57327278082555 #Duplication Level Percentage of deduplicated Percentage of total 1 84.99557164691215 56.58433376412084 2 9.1408040376234 12.170664812655485 3 2.383478334447627 4.760278599791088 4 1.0349295161895362 2.7559457996085523 5 0.5730242203727003 1.9074048866445834 6 0.34155671464968385 1.3643129000697598 7 0.261985573815048 1.2208865949161207 8 0.18571631273079392 0.9890994197820999 9 0.14881942668323853 0.8916656658902375 >10 0.8531942917047517 10.262215147321905 >50 0.055753441060672704 2.5177623989012172 >100 0.022843423766413545 2.7703634584352894 >500 7.743533480140185E-4 0.3276081090129562 >1k 0.001548706696028037 1.4774584428499329 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1765 0.4545407721784993 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1517 0.39067328690922576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.3729037043141456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1002 0.25804524290246816 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 722 0.185936791791998 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 549 0.14138407021302896 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 476 0.12258436688779926 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 457 0.11769129341958878 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2876509126869668E-4 0.0 12-13 0.0 0.0 0.0 2.5753018253739336E-4 0.0 14-15 0.0 0.0 0.0 2.5753018253739336E-4 0.0 16-17 0.0 0.0 0.0 2.5753018253739336E-4 0.0 18-19 0.0 0.0 0.0 3.8629527380609004E-4 0.0 20-21 0.0 0.0 0.0 5.150603650747867E-4 0.0 22-23 0.0 0.0 0.0 6.438254563434834E-4 0.0 24-25 0.0 0.0 0.0 9.013556388808768E-4 0.0 26-27 0.0 0.0 0.0 0.0019314763690304504 0.0 28-29 0.0 0.0 0.0 0.004120482920598295 0.0 30-31 0.0 0.0 0.0 0.01249021385306358 0.0 32-33 0.0 0.0 0.0 0.027555729531501094 0.0 34-35 0.0 0.0 0.0 0.048029379043223866 0.0 36-37 0.0 0.0 0.0 0.07867547076517367 0.0 38-39 0.0 0.0 0.0 0.12644731962586014 0.0 40-41 0.0 0.0 0.0 0.1825888994190119 0.0 42-43 0.0 0.0 0.0 0.24233590176768716 0.0 44-45 0.0 0.0 0.0 0.32165519798920433 0.0 46-47 0.0 0.0 0.0 0.4068976884090816 0.0 48-49 0.0 0.0 0.0 0.503085211586798 0.0 50-51 0.0 0.0 0.0 0.6115054184350406 0.0 52-53 0.0 0.0 0.0 0.7254625242078372 0.0 54-55 0.0 0.0 0.0 0.8446989987226503 0.0 56-57 0.0 0.0 0.0 0.9923925584078455 0.0 58-59 0.0 0.0 0.0 1.1430477151922205 0.0 60-61 0.0 0.0 0.0 1.3383843586468336 0.0 62-63 0.0 0.0 0.0 1.536425069018089 0.0 64-65 0.0 0.0 0.0 1.7454108121471836 0.0 66-67 0.0 0.0 0.0 1.9801495735300179 0.0 68-69 0.0 0.0 0.0 2.2430878899006963 0.0 70-71 0.0 0.0 0.0 2.5329381103465325 0.0 72-73 0.0 0.0 0.0 2.8369524908319255 0.0 74-75 0.0 0.0 0.0 3.169166426305163 0.0 76-77 0.0 0.0 0.0 3.529193621492439 0.0 78-79 0.0 0.0 0.0 3.923472330957188 0.0 80-81 0.0 0.0 0.0 4.367969426016729 0.0 82-83 0.0 0.0 0.0 4.828690922576126 0.0 84-85 0.0 0.0 0.0 5.275505789278503 0.0 86-87 0.0 0.0 0.0 5.766615847377313 0.0 88-89 0.0 0.0 0.0 6.2680271127776175 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 55 0.0025461565 34.631805 1 GTATGGT 90 2.368103E-5 31.745823 1 CGCACTG 80 4.510804E-4 29.66207 5 TAAGGTG 120 5.405549E-6 27.684597 5 GTACTAT 70 0.008283327 27.210705 1 ACTGCGG 90 8.981662E-4 26.366283 8 GTAAGGT 115 1.2870846E-4 24.76138 4 TATACCA 155 1.4171565E-6 24.495129 5 GGTAAGG 100 0.0016599403 23.729654 3 CAACCGT 120 1.7179281E-4 23.729654 6 TAGCATG 120 1.7179281E-4 23.729654 4 TACTAGA 100 0.0016599403 23.729654 2 GTACATG 2275 0.0 23.024443 1 AACTGCG 105 0.002204574 22.599672 7 GAGTACT 1640 0.0 21.85146 12-13 TACATGG 2300 0.0 21.666206 2 ACATGGG 2265 0.0 20.743805 3 AGTACTT 1705 0.0 20.600828 12-13 GTACTTT 1760 0.0 20.226742 14-15 CATGGGA 1245 0.0 19.822363 4 >>END_MODULE