FastQCFastQC Report
Fri 27 May 2016
SRR939271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024725
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28070.2739271511869038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.21264241625802044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.16306814023274535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.039.6958051
GTATCAA41500.033.1638031
ATCAACG49500.027.5946853
TCAACGC50500.027.0482564
TATCAAC51300.026.7189032
CAACGCA51600.026.379735
AACGCAG53800.025.2849256
GTACATG44300.025.2826541
TACATGG45200.024.7634832
CGGTTAC1550.022.97449328-29
ACATGGG46950.022.9312863
GAGTACT27950.022.72290212-13
ACGCAGA60750.022.2362047
CGCAGAG61250.021.8999168
GTACTTT29050.021.37354914-15
GCAGAGT66200.019.7592629
GTATAAG3850.019.7230321
AGTACTT29000.019.61209912-13
CATGGGG27950.019.1749744
ATATAGG3003.785317E-918.9713482
Produced by FastQC (version 0.11.5)