FastQCFastQC Report
Fri 27 May 2016
SRR939271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024725
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.2970553075215302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.24357754519505231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.1660933421161775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.033.751221
GTATCAA39650.032.5322151
TATCAAC50350.025.5437132
ATCAACG50000.025.5326863
TCAACGC50500.025.2798884
GTACATG43000.024.9058651
CAACGCA51450.024.905355
AACGCAG53200.024.17536
TACATGG44650.024.0215592
ACGTAAC1000.001633827523.7989391
CGCGGAA1353.8198777E-1122.95681882-83
ACATGGG46350.022.1165943
GTATAGG2205.5162673E-821.6353991
GAGTACT29600.021.2451412-13
ACGCAGA60400.021.2149037
CGCAGAG60800.021.075338
GTAACAC2751.2714736E-920.7091733
CGTAACA1150.003741896220.634142
TATAGGG1908.312329E-619.9825362
AGTACTT31450.019.84451712-13
Produced by FastQC (version 0.11.5)