##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939271_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1024725 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31865720071239 33.0 31.0 34.0 31.0 34.0 2 32.52984361658006 34.0 31.0 34.0 31.0 34.0 3 32.618470321305715 34.0 31.0 34.0 31.0 34.0 4 36.141113957403206 37.0 35.0 37.0 35.0 37.0 5 36.079180267876744 37.0 35.0 37.0 35.0 37.0 6 36.10657103125229 37.0 35.0 37.0 35.0 37.0 7 36.04719998048257 37.0 35.0 37.0 35.0 37.0 8 36.02139598428847 37.0 35.0 37.0 35.0 37.0 9 37.729505965015 39.0 38.0 39.0 35.0 39.0 10-11 37.6499290053429 39.0 37.0 39.0 35.0 39.0 12-13 37.570535021591155 39.0 37.0 39.0 35.0 39.0 14-15 38.87726072848813 40.0 38.0 41.0 35.0 41.0 16-17 38.87499914611237 40.0 38.0 41.0 35.0 41.0 18-19 38.96489009246383 40.0 38.0 41.0 35.0 41.0 20-21 38.93857961892215 40.0 38.0 41.0 35.0 41.0 22-23 38.85667618141453 40.0 38.0 41.0 35.0 41.0 24-25 38.78310473541681 40.0 38.0 41.0 34.0 41.0 26-27 38.62106174827393 40.0 38.0 41.0 34.0 41.0 28-29 38.448265144307015 40.0 38.0 41.0 34.0 41.0 30-31 38.25040474273585 40.0 38.0 41.0 34.0 41.0 32-33 38.21213398716729 40.0 38.0 41.0 34.0 41.0 34-35 38.118491302544584 40.0 38.0 41.0 33.0 41.0 36-37 37.97227939203201 40.0 37.5 41.0 33.0 41.0 38-39 37.84152431140062 40.0 37.0 41.0 33.0 41.0 40-41 37.64405035497329 40.0 37.0 41.0 33.0 41.0 42-43 37.45392666325112 40.0 37.0 41.0 32.0 41.0 44-45 37.21302154236503 40.0 36.5 41.0 31.5 41.0 46-47 36.898324428505205 40.0 36.0 41.0 31.0 41.0 48-49 36.572040791431846 39.0 36.0 41.0 30.0 41.0 50-51 36.303594135011835 39.0 35.0 41.0 30.0 41.0 52-53 36.01560808997536 39.0 35.0 40.0 29.0 41.0 54-55 35.732136426846225 38.5 35.0 40.0 28.5 41.0 56-57 35.386206055283125 38.0 34.5 40.0 28.0 41.0 58-59 35.033870550635534 38.0 34.0 40.0 27.5 41.0 60-61 34.662669496694235 37.0 34.0 40.0 26.5 41.0 62-63 34.279618922149844 37.0 34.0 39.5 26.0 41.0 64-65 33.932869306399276 36.0 33.0 39.0 26.0 41.0 66-67 33.49333333333333 36.0 33.0 39.0 25.5 40.0 68-69 33.00395520749469 35.0 32.5 38.5 24.5 40.0 70-71 32.512419429603064 35.0 32.0 37.5 23.5 40.0 72-73 32.01872502378687 35.0 31.0 37.0 22.0 39.0 74-75 31.497490058308326 35.0 31.0 36.5 20.5 39.0 76-77 29.596799141233014 32.5 28.5 35.0 18.0 36.5 78-79 30.556809875820342 34.0 30.0 35.0 18.0 37.0 80-81 30.580993437263658 34.0 31.0 35.0 18.0 37.0 82-83 30.23767059454976 34.0 30.0 35.0 17.0 36.5 84-85 29.796011612871744 34.0 30.0 35.0 9.5 36.0 86-87 29.188344677840398 34.0 29.0 35.0 4.5 35.5 88-89 28.604804703700992 33.5 29.0 35.0 2.0 35.0 90-91 28.114215521237405 33.0 27.5 35.0 2.0 35.0 92-93 27.7286013320647 33.0 27.0 35.0 2.0 35.0 94-95 27.186944790065628 33.0 26.5 35.0 2.0 35.0 96-97 26.407569835809607 32.5 25.0 34.0 2.0 35.0 98-99 25.35478103881529 32.0 21.5 34.0 2.0 35.0 100-101 20.979523774671254 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.267647620387379 1101 2 0.27183661607163856 1101 3 0.3843697248835838 1101 4 0.21569025696725674 1101 5 0.3066172507672249 1101 6 0.1793734376680476 1101 7 0.2669980497870199 1101 8 0.25212410914331684 1101 9 0.3573720319412672 1101 10-11 0.5213743667342641 1101 12-13 0.40408347862614846 1101 14-15 0.2758246559925439 1101 16-17 0.46060653841311705 1101 18-19 0.4880721849280363 1101 20-21 0.5457000949716502 1101 22-23 0.4918840872662429 1101 24-25 0.5443395122746324 1101 26-27 0.5062541702224763 1101 28-29 0.5998092531407835 1101 30-31 0.7194369099182083 1101 32-33 0.6454624583647046 1101 34-35 0.7449434573956708 1101 36-37 0.6998109332634002 1101 38-39 0.5959677596306179 1101 40-41 0.6199033854305824 1101 42-43 0.8378250528965978 1101 44-45 0.8736534262490991 1101 46-47 1.0170413006423686 1101 48-49 0.960163852622351 1101 50-51 0.9862536696870094 1101 52-53 0.9088316642206138 1101 54-55 0.7937767871732646 1101 56-57 1.0185545709473018 1101 58-59 0.943224181602325 1101 60-61 0.8824648027587045 1101 62-63 0.7619802497892394 1101 64-65 0.9086759427101541 1101 66-67 0.9695493641812405 1101 68-69 0.9255601709724317 1101 70-71 0.8913256179955837 1101 72-73 1.007385681369236 1101 74-75 1.223624820748796 1101 76-77 1.2141986890010372 1101 78-79 1.3450627259823733 1101 80-81 1.6199481852733228 1101 82-83 1.5256603112982532 1101 84-85 1.5602830938949133 1101 86-87 1.1044285380812155 1101 88-89 1.266431970123449 1101 90-91 1.371517359067031 1101 92-93 1.2634486072447118 1101 94-95 1.1263683059724379 1101 96-97 1.2130508312666848 1101 98-99 1.015485315448359 1101 100-101 0.7995957746666882 1102 1 0.1620100728913343 1102 2 0.08095092704001416 1102 3 0.1854186582840569 1102 4 0.07526746731620193 1102 5 0.06642370987748336 1102 6 0.14814841821183933 1102 7 0.19585933022293744 1102 8 0.12290081991073265 1102 9 0.22117737356652611 1102 10-11 0.2672674332797911 1102 12-13 0.25974068775430936 1102 14-15 0.3686134736300275 1102 16-17 0.382540468286237 1102 18-19 0.27881607544266984 1102 20-21 0.23933723867060053 1102 22-23 0.3111438004059721 1102 24-25 0.34885300132809505 1102 26-27 0.1995080588237741 1102 28-29 0.29301090636123917 1102 30-31 0.145396485602582 1102 32-33 0.3135975848388881 1102 34-35 0.4365674847318033 1102 36-37 0.30385207361458555 1102 38-39 0.33338343470541787 1102 40-41 0.34772124946130134 1102 42-43 0.284077780581228 1102 44-45 0.32950983412172974 1102 46-47 0.10025519689703088 1102 48-49 0.43731351461836 1102 50-51 0.509717333230121 1102 52-53 0.4584775787682318 1102 54-55 0.46509827187886543 1102 56-57 0.41532161171320325 1102 58-59 0.29965553081287766 1102 60-61 0.20193287050739883 1102 62-63 0.3245723219844052 1102 64-65 0.40204263625749803 1102 66-67 0.5253866386447115 1102 68-69 0.3929811400150456 1102 70-71 0.2724314351388841 1102 72-73 0.3690000715710511 1102 74-75 0.27896732638023636 1102 76-77 0.2866427061718042 1102 78-79 0.2507408879543114 1102 80-81 0.44658893380046294 1102 82-83 0.24072980783135023 1102 84-85 0.13571039691763787 1102 86-87 0.2996538721235389 1102 88-89 0.30452975710404573 1102 90-91 0.5128533875112531 1102 92-93 0.5287151309390339 1102 94-95 0.6595547933250181 1102 96-97 0.5198978919407189 1102 98-99 -0.02036341558403265 1102 100-101 -0.534666644148377 1103 1 0.22113695737186134 1103 2 0.2158557846053739 1103 3 0.24528375691411952 1103 4 0.21000608138017185 1103 5 0.16380752543322785 1103 6 0.2022449835978506 1103 7 0.23286187851493167 1103 8 0.22699134007851285 1103 9 0.29119495120107786 1103 10-11 0.16009210800054774 1103 12-13 0.2199701270370653 1103 14-15 0.2807049563825572 1103 16-17 0.11380959210161024 1103 18-19 0.28544895863975484 1103 20-21 0.3213981698202133 1103 22-23 0.2736553095714527 1103 24-25 0.32243365383964573 1103 26-27 0.3355940590283808 1103 28-29 0.3058761770475158 1103 30-31 0.25314261898800083 1103 32-33 0.30262783883105726 1103 34-35 0.2957016932267251 1103 36-37 0.3933840807200468 1103 38-39 0.475619863190488 1103 40-41 0.40712540020114574 1103 42-43 0.39665834473959904 1103 44-45 0.4927254949570852 1103 46-47 0.4984432589860788 1103 48-49 0.45689130262650934 1103 50-51 0.5488781406852681 1103 52-53 0.2340691963756214 1103 54-55 0.23982379572027668 1103 56-57 0.30105187407567513 1103 58-59 0.21398490685334792 1103 60-61 0.5094252209812566 1103 62-63 0.37833988799354046 1103 64-65 0.5472795855613768 1103 66-67 0.33729374224115105 1103 68-69 0.44585412997998475 1103 70-71 0.21222222408636782 1103 72-73 0.40069329069626036 1103 74-75 0.388590383772609 1103 76-77 0.3596067464236583 1103 78-79 0.7427838694559448 1103 80-81 0.7931392067653142 1103 82-83 0.6144236919305683 1103 84-85 0.48745200103954645 1103 86-87 0.545973845524582 1103 88-89 0.6094999727788313 1103 90-91 0.7429128864091616 1103 92-93 0.3844396038425053 1103 94-95 0.42678424655828096 1103 96-97 0.1904904628325319 1103 98-99 -0.1820968354104906 1103 100-101 -0.5172866417302906 1104 1 0.19372727555041536 1104 2 0.13260325282342933 1104 3 0.18950375260470764 1104 4 0.028480907207935502 1104 5 0.11313597476167558 1104 6 0.06466687543150584 1104 7 0.13023493029974276 1104 8 0.10925450175344054 1104 9 0.22752332870592085 1104 10-11 0.17514364143443117 1104 12-13 0.12464225229742709 1104 14-15 0.012192423164144373 1104 16-17 0.043992562725755135 1104 18-19 0.03897280407537096 1104 20-21 0.1698054814628165 1104 22-23 0.11839512857596901 1104 24-25 0.1437096001155993 1104 26-27 0.23741119613963235 1104 28-29 0.3028281629700871 1104 30-31 0.17808695569704724 1104 32-33 0.3698053508615118 1104 34-35 0.1801375188390253 1104 36-37 0.20711854886627634 1104 38-39 0.15109838793372177 1104 40-41 0.3999565070028339 1104 42-43 0.441139598779678 1104 44-45 0.48351411809865397 1104 46-47 0.4310592541020739 1104 48-49 0.31251239721818536 1104 50-51 0.4421258243741306 1104 52-53 0.5980891633367591 1104 54-55 0.4892716517857778 1104 56-57 0.300760090695654 1104 58-59 0.20983260490692857 1104 60-61 0.24449488987445278 1104 62-63 0.3605815025880972 1104 64-65 0.2631139962487268 1104 66-67 0.27688111565205276 1104 68-69 0.263139377412287 1104 70-71 0.199504957692632 1104 72-73 0.05044101029692172 1104 74-75 0.16342339389973404 1104 76-77 0.18683507365198793 1104 78-79 0.3178889114727532 1104 80-81 0.6485627864535992 1104 82-83 0.3904552584033105 1104 84-85 0.2301304826886188 1104 86-87 0.3234014832463359 1104 88-89 0.5433100352653639 1104 90-91 0.9228310075037527 1104 92-93 0.5504104793722036 1104 94-95 0.4737344368908225 1104 96-97 0.1178812909586533 1104 98-99 0.18455367094589903 1104 100-101 0.14862138814832804 1105 1 0.10506858130348462 1105 2 0.1708557846053722 1105 3 0.1607965774269431 1105 4 0.03957873095281883 1105 5 -0.06114973952404057 1105 6 -0.009165272812410308 1105 7 0.11957128022432784 1105 8 0.14600843409560582 1105 9 0.23940007940620234 1105 10-11 0.2210109114193486 1105 12-13 0.27885901592595275 1105 14-15 0.26747846065605785 1105 16-17 0.22004890834092095 1105 18-19 0.3089318646226715 1105 20-21 0.29009475101679527 1105 22-23 0.24788607880221747 1105 24-25 0.37553194443794524 1105 26-27 0.3707649991993236 1105 28-29 0.2594872881586241 1105 30-31 0.30944603779142454 1105 32-33 0.30792698413020503 1105 34-35 0.3602102402352756 1105 36-37 0.07193108926704639 1105 38-39 0.2814959315665604 1105 40-41 0.3797108702866083 1105 42-43 0.45101731909857534 1105 44-45 0.2515075462391394 1105 46-47 0.06951163505444669 1105 48-49 0.37605796929317137 1105 50-51 0.2689422432493771 1105 52-53 0.11985979466621899 1105 54-55 0.075657129053603 1105 56-57 0.21929973732353147 1105 58-59 0.008493453861895262 1105 60-61 0.0367756483316839 1105 62-63 0.26286980252345415 1105 64-65 0.17234368812547274 1105 66-67 0.15222963967704572 1105 68-69 0.3759609675868205 1105 70-71 0.3529487198128649 1105 72-73 0.31975311975609344 1105 74-75 0.3055134606956855 1105 76-77 0.2491152934322045 1105 78-79 0.33695053612261106 1105 80-81 0.46548963411574107 1105 82-83 0.6283339483408241 1105 84-85 0.5778152489027946 1105 86-87 0.538302905353639 1105 88-89 0.626871767650627 1105 90-91 0.7337462197424962 1105 92-93 0.6704438773467771 1105 94-95 0.7320192892933228 1105 96-97 0.6218152491573186 1105 98-99 0.45668521587156263 1105 100-101 0.7110039565603081 1106 1 0.032605850236102185 1106 2 0.10777588001383975 1106 3 0.07026884934107613 1106 4 0.07156441968269434 1106 5 0.07617584187131143 1106 6 0.13068863297173294 1106 7 0.07665535893631414 1106 8 0.08114558376764336 1106 9 0.15098922669302794 1106 10-11 0.14698586669663172 1106 12-13 0.19040344049363256 1106 14-15 0.11864721438295334 1106 16-17 0.12176029772673047 1106 18-19 0.14652280081825353 1106 20-21 0.23331925906688866 1106 22-23 0.3779710519685864 1106 24-25 0.30124005415768096 1106 26-27 0.22239588769068064 1106 28-29 0.2705949208360252 1106 30-31 0.26019887023029753 1106 32-33 0.2049708123949614 1106 34-35 0.3566488210343195 1106 36-37 0.08917020673872145 1106 38-39 0.17603975983131193 1106 40-41 0.25019834792525586 1106 42-43 0.33907784384513917 1106 44-45 0.3172260912600109 1106 46-47 0.3110426428063846 1106 48-49 0.22802278742077675 1106 50-51 0.20850097908718368 1106 52-53 0.3293037421915628 1106 54-55 0.2701979758037538 1106 56-57 0.18848727459246817 1106 58-59 0.23121557670030057 1106 60-61 0.1583767216769445 1106 62-63 0.15698727419906788 1106 64-65 0.40468864936578797 1106 66-67 0.3992347082518819 1106 68-69 0.38416162352003624 1106 70-71 -1.237309921506835E-4 1106 72-73 0.07400625034642871 1106 74-75 0.14377076540647948 1106 76-77 0.5617658599199835 1106 78-79 0.5039272803086554 1106 80-81 0.1513557641097769 1106 82-83 0.390288331167298 1106 84-85 0.35826545760881956 1106 86-87 0.4904182899690248 1106 88-89 0.6753169077818129 1106 90-91 0.8051147349482193 1106 92-93 1.057914062200684 1106 94-95 1.2569000287089445 1106 96-97 0.9833745812980439 1106 98-99 1.2828330989961891 1106 100-101 0.993533771706403 1107 1 0.08305176161364614 1107 2 0.03859644247641825 1107 3 0.08303097292111516 1107 4 0.06541418603602267 1107 5 0.09343401172971966 1107 6 0.11100652463120753 1107 7 -0.040881359746862245 1107 8 0.09702558812178808 1107 9 0.12033622116481268 1107 10-11 0.23861346266281913 1107 12-13 0.1531521517436616 1107 14-15 0.02493390390889516 1107 16-17 0.16960732718720095 1107 18-19 0.12767167379377042 1107 20-21 0.045113653879226945 1107 22-23 0.16954490142392586 1107 24-25 0.06347783338683399 1107 26-27 -0.01644174925115749 1107 28-29 0.1656740535831034 1107 30-31 0.18822082368756554 1107 32-33 0.11748694606729515 1107 34-35 0.06224866319646338 1107 36-37 0.19517620924236212 1107 38-39 0.16292483365744914 1107 40-41 0.1989501265168414 1107 42-43 0.1675093593218051 1107 44-45 0.2726952117999275 1107 46-47 0.14453002355327982 1107 48-49 0.06660345189482797 1107 50-51 0.19796160903904791 1107 52-53 0.24412232584951 1107 54-55 0.2763355746467937 1107 56-57 0.3703570117108015 1107 58-59 0.4803068427464936 1107 60-61 0.49713621721782886 1107 62-63 0.4178794468410203 1107 64-65 0.4468806322913039 1107 66-67 0.38391598075155287 1107 68-69 0.36974385470972493 1107 70-71 0.30625324878439386 1107 72-73 0.4072535698242419 1107 74-75 0.4114152686837542 1107 76-77 0.5461541021660992 1107 78-79 0.595278854410811 1107 80-81 0.5755901922002522 1107 82-83 0.771595527951451 1107 84-85 0.4808901145253053 1107 86-87 0.7367196941816623 1107 88-89 0.5965685503062872 1107 90-91 0.9206464074852114 1107 92-93 0.8937651231354273 1107 94-95 1.305544994614415 1107 96-97 1.2489746761562621 1107 98-99 0.9826554342189091 1107 100-101 0.46635873885591295 1108 1 -0.03976784663639421 1108 2 0.023584932851761664 1108 3 -0.07003454547314902 1108 4 0.060359750699355175 1108 5 0.06743263595488713 1108 6 0.03981498065060407 1108 7 0.019518229826452682 1108 8 -0.023561268232718646 1108 9 0.04296329779700869 1108 10-11 0.052425588696095815 1108 12-13 0.018747020641548318 1108 14-15 0.0012877739481282902 1108 16-17 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19.0 6 45.0 7 119.0 8 287.0 9 589.0 10 906.0 11 1383.0 12 1986.0 13 2745.0 14 3337.0 15 4550.0 16 5880.0 17 8269.0 18 8144.0 19 5933.0 20 5957.0 21 6161.0 22 6678.0 23 7566.0 24 8484.0 25 10028.0 26 11988.0 27 14538.0 28 17672.0 29 21488.0 30 26222.0 31 31876.0 32 39225.0 33 50532.0 34 70183.0 35 102001.0 36 160478.0 37 233535.0 38 148030.0 39 7878.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.203328550483214 18.646166313988562 14.90193852243209 23.248566613096138 2 16.304081582863695 19.870111493327478 38.81343775159189 25.012369172216935 3 19.795847666447095 23.450389128790654 29.038034594647343 27.71572861011491 4 13.92051526019176 16.35024030837542 34.9757251945644 34.75351923686843 5 14.919514991827077 36.5819122203518 32.94522920783625 15.553343579984874 6 33.84459245163337 34.5950376930396 16.546683256483448 15.013686598843593 7 30.257483715143085 31.729098050696532 19.438385908414453 18.575032325745934 8 27.37758910927322 34.68140232745371 18.736734245773256 19.204274317499817 9 26.675742272316967 16.92161311571397 19.35006953084974 37.05257508111933 10-11 26.051428432018348 26.01971260582107 25.952572641440387 21.97628632072019 12-13 26.669008758447386 23.00231769499134 26.90756056502964 23.421112981531632 14-15 23.461892405415348 24.539214241590603 24.999048517785884 26.999844835208158 16-17 23.369562830731397 27.817032499200998 25.639993461612594 23.173411208455015 18-19 23.38798214155017 26.09383005196516 27.811949547439557 22.706238259045108 20-21 23.969406426114322 25.36685452194491 27.908219278342973 22.755519773597793 22-23 23.661860499158312 25.077020664080607 27.98970455488058 23.271414281880503 24-25 23.5745199931689 25.44185025250677 27.851179584766644 23.132450169557686 26-27 23.377052379906804 25.648198297104102 27.826929176120423 23.14782014686867 28-29 23.2645831808534 25.838737222181564 27.890848764302618 23.005830832662422 30-31 23.29483519968772 25.532606309009736 27.805118446412454 23.367440044890092 32-33 23.182317207055554 25.66244602210349 27.763009587938225 23.39222718290273 34-35 23.641581069403436 25.653854767902967 27.524137281566567 23.18042688112703 36-37 23.736195956783483 25.415444913383002 27.506783994019447 23.34157513581407 38-39 23.423389135140305 25.56567886343098 27.65819782999479 23.352734171433926 40-41 23.486790677793252 25.496319385415923 27.79920424946789 23.217685687322934 42-43 23.54237651193077 25.544621993521183 27.3473016405873 23.565699853960748 44-45 23.42623237984824 25.72726354511427 27.294822118746247 23.55168195629124 46-47 23.650365660149777 25.95274620823989 26.98541653248393 23.411471599126397 48-49 23.752146927941293 25.988953079865716 26.72705714731829 23.5318428448747 50-51 23.608710056908034 26.161203485623197 26.688467669571352 23.541618787897416 52-53 23.8945061250699 26.072558144981617 26.54078224066406 23.49215348928442 54-55 23.56900631876845 25.993266486130423 26.66168972163263 23.77603747346849 56-57 23.547548784248516 26.25815347862237 26.632696278773828 23.56160145835529 58-59 23.707798829907144 25.86881102366047 26.636153819879866 23.78723632655252 60-61 23.57897489845987 25.999982434191647 26.91423396485667 23.506808702491806 62-63 23.435784584006285 26.10428265420141 26.945199396905483 23.514733364886823 64-65 23.277171924174777 25.94457049452292 26.83397984825197 23.94427773305033 66-67 23.34092402847804 26.050104443215922 26.71482293447134 23.894148593834704 68-69 23.26315578887891 25.998123372406155 26.91408939402885 23.824631444686084 70-71 23.390763375539777 25.96886969674791 26.743370172485303 23.89699675522701 72-73 23.423015931103468 26.060504037668643 26.621971748517897 23.894508282709996 74-75 23.362658274171118 26.416697162653396 26.285198467881628 23.93544609529386 76-77 23.424945615246028 26.097980120641946 26.523665867507145 23.953408396604882 78-79 23.368934876451796 26.1569119024047 26.54638357836739 23.927769642776113 80-81 23.405473083483944 26.424969064840877 26.451512797592326 23.718045054082857 82-83 23.443655614921077 26.229817755983316 26.45714703944961 23.869379589646 84-85 23.408085096001365 26.141745346312423 26.340774354095004 24.10939520359121 86-87 23.407965472337526 26.09552353701437 26.521306744636558 23.97520424601155 88-89 23.22242225025662 26.06026829440296 26.43652886280471 24.280780592535713 90-91 23.215904659718113 26.185013887808427 26.475099553349292 24.123981899124168 92-93 23.212666813047402 25.93681231549928 26.501500402547023 24.34902046890629 94-95 23.069555246529557 25.748713069360075 26.671448437385642 24.510283246724732 96-97 23.204664666129936 25.670740930493547 26.63597550562346 24.488618897753057 98-99 22.912413488900427 25.8886295236571 26.39803498307837 24.8009220043641 100-101 23.13779124915587 25.144282753209644 27.090221366924165 24.62770463071032 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 237.0 1 166.5 2 130.0 3 170.5 4 157.0 5 140.5 6 246.5 7 558.5 8 869.0 9 985.0 10 924.5 11 815.0 12 825.0 13 887.5 14 940.5 15 1019.0 16 1076.5 17 1156.5 18 1239.5 19 1295.0 20 1426.0 21 1577.5 22 1811.0 23 2152.0 24 2568.5 25 3131.0 26 3796.5 27 4716.5 28 5692.0 29 6779.0 30 8215.5 31 9874.0 32 11722.5 33 12934.0 34 13958.0 35 15268.0 36 17011.5 37 19144.5 38 21144.5 39 22650.5 40 23960.5 41 26409.0 42 28544.5 43 30426.0 44 32633.0 45 34772.5 46 35761.0 47 37245.0 48 41070.5 49 42564.5 50 41554.0 51 41106.0 52 41147.5 53 41767.0 54 42469.5 55 40907.0 56 37199.0 57 33895.5 58 31375.0 59 27843.5 60 23516.0 61 19640.0 62 16398.5 63 12968.0 64 9783.5 65 7471.0 66 5557.0 67 4408.0 68 3317.5 69 2291.0 70 1742.0 71 1342.5 72 1050.5 73 905.0 74 752.5 75 576.0 76 417.0 77 246.0 78 148.0 79 97.0 80 48.0 81 37.0 82 28.5 83 15.5 84 11.0 85 6.0 86 3.5 87 2.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29461562858327844 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.806972602405524E-4 16-17 7.319036814755177E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.001317426626655932 36-37 0.006879894605869868 38-39 0.002634853253311864 40-41 0.0031227890409622097 42-43 4.8793578765034527E-5 44-45 0.001024665154065725 46-47 2.927614725902071E-4 48-49 4.879357876503452E-4 50-51 1.4638073629510355E-4 52-53 1.951743150601381E-4 54-55 0.0 56-57 8.782844177706214E-4 58-59 0.001951743150601381 60-61 6.831101027104834E-4 62-63 9.758715753006904E-4 64-65 0.0 66-67 5.367293664153798E-4 68-69 0.0014150137841860012 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2546536875747152 78-79 3.415550513552417E-4 80-81 9.758715753006905E-5 82-83 0.0 84-85 0.0 86-87 0.1055893044475347 88-89 0.3666349508404694 90-91 0.2718290273000073 92-93 0.0 94-95 0.0 96-97 0.0 98-99 9.758715753006905E-5 100-101 9.758715753006905E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1024725.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90029873440821 #Duplication Level Percentage of deduplicated Percentage of total 1 80.25422025358846 49.67760208394119 2 11.452796326008224 14.178630278484839 3 3.4891532587597154 6.479388871420808 4 1.5610351182929418 3.8651416062894137 5 0.8668230616188137 2.6828303232039454 6 0.5088063199642355 1.889715792224464 7 0.3536663607461333 1.5324437367747639 8 0.2587133091310416 1.2811544897423022 9 0.18380844535982516 1.0240017909912291 >10 0.9827243911153027 10.810767646653526 >50 0.05679041289462076 2.3390197722547588 >100 0.030197909197379448 3.244192453364298 >500 7.905208269971795E-4 0.28635869773004025 >1k 4.7431249619830766E-4 0.7087524569244537 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3044 0.2970553075215302 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2496 0.24357754519505231 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1702 0.1660933421161775 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.758715753006904E-5 14-15 0.0 0.0 0.0 0.0 1.9517431506013808E-4 16-17 0.0 0.0 0.0 0.0 1.9517431506013808E-4 18-19 0.0 0.0 0.0 9.758715753006904E-5 2.9276147259020715E-4 20-21 0.0 0.0 0.0 9.758715753006904E-5 4.879357876503452E-4 22-23 0.0 0.0 0.0 9.758715753006904E-5 5.855229451804143E-4 24-25 0.0 0.0 0.0 2.9276147259020715E-4 5.855229451804143E-4 26-27 0.0 0.0 0.0 4.391422088853107E-4 6.831101027104832E-4 28-29 0.0 0.0 0.0 0.0017565688355412425 6.831101027104832E-4 30-31 0.0 0.0 0.0 0.005513674400448901 6.831101027104832E-4 32-33 0.0 0.0 0.0 0.013564614896679598 6.831101027104832E-4 34-35 0.0 0.0 0.0 0.027129229793359196 7.806972602405523E-4 36-37 0.0 0.0 0.0 0.046549074141842936 7.806972602405523E-4 38-39 0.0 0.0 0.0 0.07631315718851399 7.806972602405523E-4 40-41 0.0 0.0 0.0 0.10900485496108711 7.806972602405523E-4 42-43 0.0 0.0 0.0 0.1461855619800434 9.27077996535656E-4 44-45 0.0 0.0 0.0 0.18902632413574372 0.0010734587328307594 46-47 0.0 0.0 0.0 0.24186976993827614 0.0011710458903608286 48-49 0.0 0.0 0.0 0.302422601185684 0.0011710458903608286 50-51 0.0 0.0 0.0 0.3667813315767645 0.0012686330478908975 52-53 0.0 0.0 0.0 0.4328478372246213 0.0014638073629510357 54-55 0.0 0.0 0.0 0.510185659567201 0.0014638073629510357 56-57 0.0 0.0 0.0 0.6057722803679035 0.0014638073629510357 58-59 0.0 0.0 0.0 0.7143379931201055 0.0014638073629510357 60-61 0.0 0.0 0.0 0.8346141647759155 0.0015613945204811046 62-63 0.0 0.0 0.0 0.9588426163116934 0.0015613945204811046 64-65 0.0 0.0 0.0 1.0879504257239747 0.0015613945204811046 66-67 0.0 0.0 0.0 1.2432115933543146 0.0016101880992461393 68-69 0.0 0.0 0.0 1.4095976969430823 0.0016589816780111738 70-71 0.0 0.0 0.0 1.6046256312669254 0.0017565688355412428 72-73 0.0 0.0 0.0 1.800629437166069 0.0017565688355412428 74-75 0.0 0.0 0.0 2.0069774817634 0.001854155993071312 76-77 0.0 0.0 0.0 2.2455780819244184 0.002000536729366415 78-79 0.0 0.0 0.0 2.516431237649125 0.00204933030813145 80-81 0.0 0.0 0.0 2.8083144258215618 0.00204933030813145 82-83 0.0 0.0 0.0 3.112786357315377 0.0022445046231915878 84-85 0.0 0.0 0.0 3.4341408670618945 0.0022445046231915878 86-87 0.0 0.0 0.0 3.77389055600283 0.0022445046231915878 88-89 0.0 0.0 0.0 4.150576984068897 0.0022445046231915878 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2750 0.0 33.75122 1 GTATCAA 3965 0.0 32.532215 1 TATCAAC 5035 0.0 25.543713 2 ATCAACG 5000 0.0 25.532686 3 TCAACGC 5050 0.0 25.279888 4 GTACATG 4300 0.0 24.905865 1 CAACGCA 5145 0.0 24.90535 5 AACGCAG 5320 0.0 24.1753 6 TACATGG 4465 0.0 24.021559 2 ACGTAAC 100 0.0016338275 23.798939 1 CGCGGAA 135 3.8198777E-11 22.956818 82-83 ACATGGG 4635 0.0 22.116594 3 GTATAGG 220 5.5162673E-8 21.635399 1 GAGTACT 2960 0.0 21.24514 12-13 ACGCAGA 6040 0.0 21.214903 7 CGCAGAG 6080 0.0 21.07533 8 GTAACAC 275 1.2714736E-9 20.709173 3 CGTAACA 115 0.0037418962 20.63414 2 TATAGGG 190 8.312329E-6 19.982536 2 AGTACTT 3145 0.0 19.844517 12-13 >>END_MODULE