FastQCFastQC Report
Fri 27 May 2016
SRR939270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040988
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.2809830660872171No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23150.2223848882023616No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.14515056849838806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.035.6765331
GTATCAA38150.035.48951
TATCAAC48300.028.075812
ATCAACG48150.027.9663263
TCAACGC48350.027.9473654
GTACATG42450.027.3062271
CAACGCA50000.027.02515
TACATGG42550.026.5210532
AACGCAG51350.026.2096736
ACATGGG44350.024.8032023
GAGTACT29650.022.61598212-13
ACGCAGA59100.022.612337
CGCAGAG60100.022.1572328
CATGGGG25300.021.7385184
GTACTTT30450.021.63272314-15
GCAGAGT63950.020.3786729
GTCTATA2351.13006536E-720.2153681
GTGTTAG3353.45608E-1119.8533021
GTGTAGC4550.019.8377191
AGTACTT31900.019.68379812-13
Produced by FastQC (version 0.11.5)