##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939270_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040988 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765529477765355 33.0 31.0 34.0 30.0 34.0 2 31.843302708580694 33.0 31.0 34.0 30.0 34.0 3 31.897344637978534 34.0 31.0 34.0 30.0 34.0 4 35.39321586800232 37.0 35.0 37.0 33.0 37.0 5 35.4262268152947 37.0 35.0 37.0 33.0 37.0 6 35.5146034344296 37.0 35.0 37.0 33.0 37.0 7 35.42273974339762 37.0 35.0 37.0 33.0 37.0 8 35.40697203041726 37.0 35.0 37.0 33.0 37.0 9 37.022584314132345 39.0 37.0 39.0 33.0 39.0 10-11 36.92455628691205 39.0 37.0 39.0 33.0 39.0 12-13 36.8128249316995 39.0 37.0 39.0 33.0 39.0 14-15 38.038136846918505 40.0 38.0 41.0 33.0 41.0 16-17 37.96731038206012 40.0 38.0 41.0 33.0 41.0 18-19 37.880008703270356 40.0 38.0 41.0 32.5 41.0 20-21 37.76291753603307 40.0 38.0 41.0 32.0 41.0 22-23 37.6801874757442 40.0 38.0 41.0 32.0 41.0 24-25 37.697947526772644 40.0 38.0 41.0 32.0 41.0 26-27 37.61577991292887 40.0 38.0 41.0 32.0 41.0 28-29 37.466426606262516 40.0 37.0 41.0 31.5 41.0 30-31 37.288738679024156 40.0 37.0 41.0 31.0 41.0 32-33 37.090638893051604 40.0 36.5 41.0 30.5 41.0 34-35 36.78292305002556 40.0 36.0 41.0 30.0 41.0 36-37 36.52965211894853 39.5 36.0 41.0 30.0 41.0 38-39 36.20087215222462 39.0 35.5 41.0 29.5 41.0 40-41 36.058372430806116 39.0 35.0 41.0 29.0 41.0 42-43 35.880022152032495 39.0 35.0 41.0 28.0 41.0 44-45 35.862692941705376 39.0 35.0 41.0 28.0 41.0 46-47 35.67545831460113 39.0 35.0 41.0 27.0 41.0 48-49 35.46287517243235 39.0 35.0 41.0 26.0 41.0 50-51 34.29989586815602 38.0 34.0 40.0 24.5 40.5 52-53 34.39814387869985 38.0 33.5 39.5 24.0 40.5 54-55 34.86733468589455 38.0 34.0 40.0 25.0 41.0 56-57 34.74089086521651 38.0 34.0 40.0 24.5 41.0 58-59 34.497662797265676 38.0 34.0 40.0 24.0 41.0 60-61 34.17476041990878 37.5 34.0 40.0 23.0 41.0 62-63 33.78531308718256 37.0 33.5 40.0 22.5 41.0 64-65 33.38327483121804 36.5 33.0 39.5 22.0 41.0 66-67 33.0140611611277 36.0 33.0 39.0 20.5 41.0 68-69 32.593418463997665 35.0 32.5 39.0 20.0 40.5 70-71 32.16951011923288 35.0 32.0 38.0 18.5 40.0 72-73 31.732332169054782 35.0 31.5 37.0 17.5 39.5 74-75 31.28793895798991 35.0 31.0 37.0 13.0 39.0 76-77 30.855425807021792 35.0 31.0 36.0 9.0 39.0 78-79 30.43640272510346 35.0 30.5 36.0 7.0 37.5 80-81 30.002110975342653 34.0 30.0 35.0 3.5 37.0 82-83 29.587023097288345 34.0 30.0 35.0 2.0 37.0 84-85 29.18920679200913 34.0 29.0 35.0 2.0 36.0 86-87 28.85818424419878 34.0 29.0 35.0 2.0 36.0 88-89 28.45311905612745 34.0 29.0 35.0 2.0 35.5 90-91 28.061759117300106 34.0 28.5 35.0 2.0 35.0 92-93 27.650235161212233 34.0 27.0 35.0 2.0 35.0 94-95 27.17585409245832 33.5 26.5 35.0 2.0 35.0 96-97 26.59718075520563 33.0 25.0 35.0 2.0 35.0 98-99 25.834404431175 33.0 23.5 35.0 2.0 35.0 100-101 23.3420332414975 30.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2557318110303264 1101 2 0.1433173982937248 1101 3 0.2570921197813689 1101 4 0.2679636788798945 1101 5 0.26032591842624697 1101 6 0.21488807132801924 1101 7 0.43310194598888785 1101 8 0.31441245537029516 1101 9 0.38555515235667315 1101 10-11 0.3841501916783443 1101 12-13 0.40380006076134833 1101 14-15 0.3667343316736478 1101 16-17 0.5728124973199513 1101 18-19 0.5105511340125375 1101 20-21 0.6621379778654344 1101 22-23 0.5989499293547595 1101 24-25 0.5830829276966298 1101 26-27 0.5503924999973151 1101 28-29 0.5698041744061939 1101 30-31 0.687037047778496 1101 32-33 0.6514722358998952 1101 34-35 0.47275105306161436 1101 36-37 0.5147624798985646 1101 38-39 0.5937988440423325 1101 40-41 0.49944539781092345 1101 42-43 0.45400242262810764 1101 44-45 0.4523834436412528 1101 46-47 0.6029027071968258 1101 48-49 0.6397883631456622 1101 50-51 0.7103985943452429 1101 52-53 0.6411719270895233 1101 54-55 0.6883528762147222 1101 56-57 0.48113681814138687 1101 58-59 0.5400640399411998 1101 60-61 0.4861523384569324 1101 62-63 0.4603197992546697 1101 64-65 0.43841249960953377 1101 66-67 0.55285691496411 1101 68-69 0.5313818352296167 1101 70-71 0.5142605320496614 1101 72-73 0.6240831433014371 1101 74-75 0.3921956107976676 1101 76-77 0.4877098093522001 1101 78-79 0.49191104499374205 1101 80-81 0.46884453583000507 1101 82-83 0.44637357118340404 1101 84-85 0.46649447478972306 1101 86-87 0.7088670693526673 1101 88-89 0.0778349018030724 1101 90-91 0.34761952217462166 1101 92-93 0.15979692414420654 1101 94-95 0.28266267384737986 1101 96-97 0.21026427707258222 1101 98-99 0.32846577792556175 1101 100-101 0.06459854778696794 1102 1 0.08629480154885627 1102 2 0.17638458515576616 1102 3 0.19883553038791035 1102 4 0.12333046198563835 1102 5 0.20851581998229562 1102 6 0.09278128005641406 1102 7 0.1780323093695486 1102 8 0.242387322675107 1102 9 0.03356812699714595 1102 10-11 0.16522581659328495 1102 12-13 0.13297031889317878 1102 14-15 0.393774661937222 1102 16-17 0.27976708607828726 1102 18-19 0.3014099100412082 1102 20-21 0.2382698564663528 1102 22-23 0.32511696650941246 1102 24-25 0.20529632877633475 1102 26-27 0.22991094122581757 1102 28-29 0.23465923043305992 1102 30-31 0.23980209790778417 1102 32-33 0.20321882211774067 1102 34-35 0.3752461762299717 1102 36-37 0.35870386718704594 1102 38-39 0.24426044182848017 1102 40-41 0.4569511819600862 1102 42-43 0.3354092184362969 1102 44-45 0.21272232673339175 1102 46-47 0.16860610510092044 1102 48-49 0.21578187582542085 1102 50-51 0.23018151862959257 1102 52-53 0.22444212624571236 1102 54-55 0.3356286553736467 1102 56-57 0.20446962220616882 1102 58-59 0.16971290806259987 1102 60-61 0.1672200243436066 1102 62-63 0.016327375355928098 1102 64-65 0.1097469368458448 1102 66-67 0.155399672302039 1102 68-69 -0.014117869892189105 1102 70-71 -0.06360318885947436 1102 72-73 0.06362517478535423 1102 74-75 -0.08444890219965728 1102 76-77 -0.004076698836342985 1102 78-79 -0.05884883342570646 1102 80-81 -0.035444807725770744 1102 82-83 -0.09083325184431246 1102 84-85 -0.1775501198661793 1102 86-87 -0.053716290444089765 1102 88-89 -0.030223211883814827 1102 90-91 -0.20682633365262504 1102 92-93 -0.011097464096955889 1102 94-95 -0.09584410835104862 1102 96-97 0.0036635421453965478 1102 98-99 -0.10549284839571627 1102 100-101 -0.562327233640243 1103 1 0.2749251659808003 1103 2 0.2715499750271704 1103 3 0.3644012300731987 1103 4 0.4099194377747537 1103 5 0.3410992493905738 1103 6 0.3044763092350067 1103 7 0.3081519171166818 1103 8 0.4125712969285047 1103 9 0.4904345553682603 1103 10-11 0.3571768873652772 1103 12-13 0.5283769718793678 1103 14-15 0.6743810246954567 1103 16-17 0.4923212256102758 1103 18-19 0.7355722329331584 1103 20-21 0.6290426551629977 1103 22-23 0.6028099213593805 1103 24-25 0.7044550238188663 1103 26-27 0.47210995213607276 1103 28-29 0.5534092026121229 1103 30-31 0.4751972219001104 1103 32-33 0.46530868595097985 1103 34-35 0.511515322594768 1103 36-37 0.44685726957563787 1103 38-39 0.4975600042608761 1103 40-41 0.5477086235027429 1103 42-43 0.3310001572703172 1103 44-45 0.269939966539134 1103 46-47 0.37099447639684513 1103 48-49 0.28536536310124205 1103 50-51 0.05798998598562122 1103 52-53 0.14485979632063106 1103 54-55 0.23031307698761339 1103 56-57 0.3497427347229163 1103 58-59 0.3589620100029478 1103 60-61 0.34098177034073274 1103 62-63 0.2992384240492356 1103 64-65 0.3242107050908061 1103 66-67 0.2797191723265229 1103 68-69 0.3279460425764853 1103 70-71 0.021385304403409577 1103 72-73 0.08534219362791617 1103 74-75 0.35600874098911106 1103 76-77 0.2989433188800241 1103 78-79 0.3290873431325956 1103 80-81 0.305703239689997 1103 82-83 0.19752179665313463 1103 84-85 0.06464110118335853 1103 86-87 -0.03671881435896296 1103 88-89 -0.1656655201753381 1103 90-91 -0.010802278562728418 1103 92-93 -0.1385429239435645 1103 94-95 -0.3604957235230266 1103 96-97 -0.5396069084646591 1103 98-99 -0.7555856590219783 1103 100-101 -0.8680659127469461 1104 1 0.09143715699849864 1104 2 0.08333617176056052 1104 3 0.10779388985109506 1104 4 0.2301440829564143 1104 5 0.1527494835969918 1104 6 0.20251099283704832 1104 7 0.1342909322216741 1104 8 0.08789716840444584 1104 9 0.2878481972341973 1104 10-11 0.21304345110977607 1104 12-13 0.20236076163744343 1104 14-15 0.25362195906502905 1104 16-17 0.3440935629376938 1104 18-19 0.29736500849850245 1104 20-21 0.3627995190776687 1104 22-23 0.48879437777242174 1104 24-25 0.29020790534726615 1104 26-27 0.3583444041632333 1104 28-29 0.21969466251633918 1104 30-31 0.2879176127704497 1104 32-33 0.3923022913263239 1104 34-35 0.28604669046551834 1104 36-37 0.30551654748303747 1104 38-39 0.4810909949452551 1104 40-41 0.1624259091643978 1104 42-43 0.32421518858555487 1104 44-45 0.29830021460495715 1104 46-47 0.2538704347133631 1104 48-49 0.35177209280774235 1104 50-51 0.46844660078185996 1104 52-53 0.5890241530863065 1104 54-55 0.5529872405987746 1104 56-57 0.366202972262613 1104 58-59 0.3870558153747723 1104 60-61 0.39208832987477393 1104 62-63 0.31251450692496974 1104 64-65 0.4013639567881526 1104 66-67 0.38782473187811206 1104 68-69 0.5424872348362655 1104 70-71 0.21616916784440576 1104 72-73 0.3034502092767646 1104 74-75 0.2677789292314223 1104 76-77 0.19650651952562725 1104 78-79 0.27781083043983656 1104 80-81 0.39598783996016707 1104 82-83 0.45467189051113976 1104 84-85 0.4708883600033822 1104 86-87 0.1498535703360453 1104 88-89 0.18461122935536878 1104 90-91 0.0019485942289740876 1104 92-93 0.14434904286403594 1104 94-95 0.05812083583558092 1104 96-97 -0.07518852106795038 1104 98-99 -0.12918217487257877 1104 100-101 -0.24176878638074228 1105 1 0.17955153944212654 1105 2 0.29513563974919066 1105 3 0.2880656388384608 1105 4 0.23300817823481168 1105 5 0.30335253057117484 1105 6 0.22398037195559795 1105 7 0.40351588839119756 1105 8 0.12760153936145713 1105 9 0.1740476410404952 1105 10-11 0.3335772761505851 1105 12-13 0.24848856527687957 1105 14-15 0.32998901667944125 1105 16-17 0.3471843676048749 1105 18-19 0.2858632855071477 1105 20-21 0.3203670784755275 1105 22-23 0.3716763075227334 1105 24-25 0.2557480782509529 1105 26-27 0.3428828238476527 1105 28-29 0.2252906059296791 1105 30-31 0.3664875299459496 1105 32-33 0.48972309417983695 1105 34-35 0.48671094897466816 1105 36-37 0.5194584191957574 1105 38-39 0.5142056796680592 1105 40-41 0.43180200492644616 1105 42-43 0.20576708992162906 1105 44-45 0.4071471706535661 1105 46-47 0.4812259452629064 1105 48-49 0.5856757262035401 1105 50-51 0.6354445150554824 1105 52-53 0.4702851107067616 1105 54-55 0.6293731906622568 1105 56-57 0.6871781249136006 1105 58-59 0.6178384161848882 1105 60-61 0.6185936802163496 1105 62-63 0.6593647578166895 1105 64-65 0.45203728367285834 1105 66-67 0.4567066011725984 1105 68-69 0.5321001663398093 1105 70-71 0.43085540205031947 1105 72-73 0.5808744883409069 1105 74-75 0.5517815590603341 1105 76-77 0.43671267392983637 1105 78-79 0.09402886441601765 1105 80-81 0.07098859565813243 1105 82-83 0.11888127930262726 1105 84-85 0.320685832949394 1105 86-87 0.2913466783706262 1105 88-89 0.335969974292194 1105 90-91 0.1638153193362868 1105 92-93 0.34061136074082654 1105 94-95 0.5587669575936367 1105 96-97 0.5024632230829482 1105 98-99 0.5058837219897647 1105 100-101 0.27367390148577186 1106 1 0.15542419759787052 1106 2 0.16392322585930685 1106 3 0.15272571800404222 1106 4 0.22343236212350348 1106 5 0.19781630550647833 1106 6 0.15164787650617484 1106 7 0.2736129260794584 1106 8 0.08616756089879374 1106 9 0.024698961636339334 1106 10-11 0.1957262832787947 1106 12-13 0.32204538247372483 1106 14-15 0.20489975934177096 1106 16-17 0.3047850684555584 1106 18-19 0.36083738540106935 1106 20-21 0.27556402246938916 1106 22-23 0.3313283793248445 1106 24-25 0.3026484237232907 1106 26-27 0.33817127467047214 1106 28-29 0.15187928335262058 1106 30-31 0.08932492268235848 1106 32-33 0.15290562904694838 1106 34-35 0.19551017465436615 1106 36-37 0.34932413075731716 1106 38-39 0.4248998437859868 1106 40-41 0.4794334350663192 1106 42-43 0.4326489235253206 1106 44-45 0.3579653457176448 1106 46-47 0.027466921500852948 1106 48-49 0.12734497256204946 1106 50-51 0.23090188409999968 1106 52-53 0.15421559110729532 1106 54-55 0.05727751092377531 1106 56-57 0.08443725392708501 1106 58-59 0.04197380666767714 1106 60-61 0.007676733911090139 1106 62-63 0.08483488085767732 1106 64-65 0.08105541168335861 1106 66-67 0.08731175560326676 1106 68-69 0.012309802417519222 1106 70-71 0.1262211404406699 1106 72-73 -0.13608946201344096 1106 74-75 -0.01383703040544404 1106 76-77 -0.06070299078452024 1106 78-79 0.20943860203040288 1106 80-81 0.18687196469440082 1106 82-83 0.1453695551191494 1106 84-85 -0.02277537054908052 1106 86-87 0.08536241242709863 1106 88-89 -0.22031941760220874 1106 90-91 -0.11576807075026707 1106 92-93 0.07934533053573034 1106 94-95 -0.033499848582764 1106 96-97 0.11102903824493282 1106 98-99 -0.021946509522098978 1106 100-101 -0.33190875803205344 1107 1 0.14082140774021568 1107 2 0.134806759995854 1107 3 0.10211499107265354 1107 4 0.1482796803906581 1107 5 0.23085972177510428 1107 6 0.21374240848022907 1107 7 0.23926250665026316 1107 8 0.17901231268700712 1107 9 0.33334775800524596 1107 10-11 0.21402135706505732 1107 12-13 0.27995096931814345 1107 14-15 0.28590425067989855 1107 16-17 0.3118993242954886 1107 18-19 0.2856549990509407 1107 20-21 0.22699697320724965 1107 22-23 0.5083649278859568 1107 24-25 0.2891595570138463 1107 26-27 0.2965788695470195 1107 28-29 0.30505114238638953 1107 30-31 0.46734885272204707 1107 32-33 0.526800617283925 1107 34-35 0.4402108709305992 1107 36-37 0.505561506294967 1107 38-39 0.563798634213974 1107 40-41 0.1309091604530792 1107 42-43 0.3153394489405841 1107 44-45 0.33668198743242783 1107 46-47 0.2633542578284107 1107 48-49 0.25129639957186356 1107 50-51 0.143745566522 1107 52-53 0.2536220928539237 1107 54-55 0.25605189841456166 1107 56-57 0.12069470978602226 1107 58-59 0.23375869771114566 1107 60-61 0.03631495543624652 1107 62-63 0.341681795142037 1107 64-65 0.5312793844976937 1107 66-67 0.5125719680506933 1107 68-69 0.446187324339526 1107 70-71 0.35828252206183464 1107 72-73 0.2510597264564147 1107 74-75 0.5365984392839636 1107 76-77 0.35648944760810153 1107 78-79 0.3628896844666549 1107 80-81 0.4105269396237041 1107 82-83 0.4708311648717718 1107 84-85 0.4790306701818885 1107 86-87 0.5922182380966383 1107 88-89 0.572178978348461 1107 90-91 0.6218777789206023 1107 92-93 0.5597581058975329 1107 94-95 0.5942567399013505 1107 96-97 0.7647860782392328 1107 98-99 0.7949037316793941 1107 100-101 0.7180365854721416 1108 1 0.008022270350867444 1108 2 0.17523024411519827 1108 3 0.1448800910303163 1108 4 0.11008580940637813 1108 5 0.1463650722771206 1108 6 0.09510022294679743 1108 7 0.31533322401809016 1108 8 0.15242875582285365 1108 9 0.19846138379852363 1108 10-11 0.254581047861663 1108 12-13 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-1.2640543615673607 2316 72-73 -1.2955296226560087 2316 74-75 -1.3823277934576517 2316 76-77 -0.9312178502222714 2316 78-79 -1.218905976282855 2316 80-81 -1.0498533365104201 2316 82-83 -0.9968264288165933 2316 84-85 -1.1397906315932573 2316 86-87 -1.0403371859664787 2316 88-89 -1.0501097790479932 2316 90-91 -1.032474094846652 2316 92-93 -1.123934990749408 2316 94-95 -1.182056475088789 2316 96-97 -1.094391042076353 2316 98-99 -0.9061384773935828 2316 100-101 -0.3880099628513314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3811.0 3 1231.0 4 1170.0 5 1408.0 6 1514.0 7 1752.0 8 2313.0 9 2779.0 10 3189.0 11 3881.0 12 4635.0 13 6037.0 14 5618.0 15 5838.0 16 7135.0 17 8860.0 18 7936.0 19 6218.0 20 6548.0 21 7070.0 22 7462.0 23 8572.0 24 9429.0 25 10567.0 26 12161.0 27 14468.0 28 16964.0 29 20124.0 30 23713.0 31 29092.0 32 36155.0 33 47222.0 34 63556.0 35 92817.0 36 144277.0 37 213394.0 38 176917.0 39 25148.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.060901480632324 18.016318745363993 14.303756008747001 23.619023765256678 2 15.705520882417995 19.318376335503476 39.7414099839403 25.23469279813823 3 19.65420600296994 23.32642784048504 28.696939270133647 28.32242688641137 4 13.469828208043118 15.647860150459824 35.36527190294553 35.51703973855153 5 15.05361864538016 36.21896452387662 32.97452909950109 15.75288773124213 6 33.846683217659006 34.137238675497706 16.96957289404962 15.046505212793667 7 30.326924925502258 31.595020667115254 19.550514274728446 18.527540132654043 8 27.40783465513098 34.20793078586878 19.01639029079548 19.36784426820476 9 26.939747778716633 16.934912528105496 19.544025293263235 36.58131439991464 10-11 26.047295365096513 25.77718993266108 26.13779510340184 22.037719598840567 12-13 26.689372026650442 22.858779774349863 26.9708338980835 23.481014300916193 14-15 23.528196087685597 24.423440435515445 25.018635904647606 27.02972757215135 16-17 23.520763524022094 27.698508363660135 25.577506839149734 23.20322127316804 18-19 23.51870757127449 26.024537766011647 27.72783901164501 22.728915651068853 20-21 23.98959651153401 25.293060752065895 27.761606109429852 22.955736626970243 22-23 23.762213942461376 25.082307563258695 27.81132784728408 23.34415064699585 24-25 23.616855268389088 25.419913053636474 27.7666860724771 23.19654560549734 26-27 23.312531578813267 25.65336594694615 27.70411992040817 23.329982553832412 28-29 23.27596691363983 25.85296115424422 27.78085165602453 23.090220276091415 30-31 23.300526117187857 25.539684714240735 27.659824018727754 23.499965149843653 32-33 23.156975408394082 25.664538617790438 27.652698962636258 23.525787011179226 34-35 23.686159185210222 25.61947460811304 27.414658242986768 23.279707963689972 36-37 23.73562933036259 25.428125320868027 27.380063448112296 23.45618190065709 38-39 23.474910806240263 25.54225902263013 27.496834904676053 23.485995266453553 40-41 23.548677137521032 25.44320277852196 27.63546161896703 23.372658464989968 42-43 23.562017650270935 25.499971323490783 27.19395778721452 23.744053239023767 44-45 23.438891082083817 25.713451129591792 27.285272961184305 23.562384827140086 46-47 23.65298643803405 25.915820751051587 26.873314636260304 23.557878174654057 48-49 23.76876614113517 25.922620898518574 26.6716997359567 23.63691322438956 50-51 23.58990452335432 26.19958022284633 26.647461428032898 23.56305382576645 52-53 23.855154750249106 26.028643366513897 26.57024392995203 23.54595795328496 54-55 23.538328802514123 25.982079164015655 26.6615092447992 23.818082788671024 56-57 23.493187454159763 26.213772358710873 26.662512734068315 23.630527453061042 58-59 23.654306854077888 25.809950294781437 26.76468944356752 23.771053407573156 60-61 23.521150367292208 25.855522098218152 27.019577358694125 23.60375017579551 62-63 23.399047067317376 26.0255882571241 26.986810663669512 23.588554011889006 64-65 23.298053485753073 25.953876830039647 26.789206044337483 23.958863639869797 66-67 23.269110332329078 26.123107022103685 26.73756700844045 23.870215637126783 68-69 23.27507188054353 25.964896546503326 27.00577214144953 23.754259431503616 70-71 23.400615360283755 25.939398439756463 26.745606239816926 23.914379960142863 72-73 23.364825704022497 26.032814609940225 26.725447497154402 23.876912188882873 74-75 23.37985919302358 26.371055261651914 26.44689220402982 23.802193341294686 76-77 23.5217984209443 26.062420855605435 26.50955806396723 23.906222659483035 78-79 23.45587087147872 26.151783459504685 26.618058407021643 23.774287261994953 80-81 23.463666150848596 26.4503829117874 26.450913026904306 23.6350379104597 82-83 23.568126936999032 26.246210350262444 26.447730548071313 23.73793216466721 84-85 23.458945543833806 26.214001251334 26.296427335381612 24.03062586945058 86-87 23.519250252804465 26.145958550671267 26.491498154455094 23.84329304206917 88-89 23.43538644723078 26.073485955042642 26.40765326044514 24.083474337281444 90-91 23.466367713004484 26.210231930179855 26.49819181252712 23.82520854428854 92-93 23.53006126864229 25.941799852554098 26.50043563562058 24.027703243183037 94-95 23.417806964252996 25.774582694477395 26.641160644545376 24.166449696724236 96-97 23.561890737400315 25.769641177267598 26.584426311320442 24.08404177401164 98-99 23.428020980130203 25.86597999279706 26.392508170805147 24.313490856267595 100-101 23.61560864439018 25.343995017820355 26.82736959617159 24.213026741617877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 812.0 1 478.5 2 177.5 3 202.0 4 185.5 5 182.0 6 293.0 7 618.5 8 954.5 9 1117.5 10 1037.5 11 913.0 12 951.5 13 1069.0 14 1184.0 15 1250.5 16 1296.0 17 1337.5 18 1403.5 19 1452.5 20 1526.0 21 1688.5 22 1957.5 23 2242.5 24 2608.5 25 3184.0 26 3908.0 27 4789.5 28 5864.0 29 6960.0 30 8270.5 31 9878.0 32 11724.5 33 12993.0 34 14023.0 35 15489.0 36 17342.5 37 19567.5 38 21544.5 39 22883.5 40 24177.5 41 26502.5 42 28622.0 43 30743.0 44 32899.0 45 35035.5 46 36292.0 47 37637.5 48 41195.0 49 42562.0 50 41598.5 51 41350.5 52 41575.5 53 42098.0 54 42680.0 55 41394.0 56 37817.0 57 34343.5 58 31866.5 59 28500.5 60 24006.0 61 20118.5 62 16877.0 63 13405.0 64 10161.0 65 7811.5 66 5938.0 67 4625.5 68 3476.5 69 2511.0 70 1946.0 71 1482.5 72 1162.0 73 972.0 74 767.0 75 587.0 76 427.0 77 276.5 78 180.0 79 123.0 80 84.5 81 52.0 82 37.0 83 29.5 84 19.0 85 7.5 86 7.0 87 8.0 88 10.0 89 8.0 90 3.5 91 5.0 92 6.0 93 4.0 94 2.0 95 2.5 96 3.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28031062798034173 2 0.10739797192666967 3 0.11873335715685485 4 0.11930973267703374 5 0.12785930289302086 6 0.054467486656906705 7 0.06609105964718133 8 0.07089418898200556 9 0.06830049914120048 10-11 0.06277690040615262 12-13 0.06772412362102156 14-15 0.06316115075293854 16-17 0.06282493169950086 18-19 0.06858868690128993 20-21 0.07300756588932822 22-23 0.0801642285982163 24-25 0.08852167364081046 26-27 0.08948229950777531 28-29 0.08275791843902139 30-31 0.07881935238446552 32-33 0.07252725295584579 34-35 0.23890765311415696 36-37 0.3614354824455229 38-39 0.3361710221443475 40-41 0.31835141231214964 42-43 0.34135840182595767 44-45 0.35432685102998307 46-47 0.3601386375251204 48-49 0.3696008023147241 50-51 0.36225201443244304 52-53 0.3635488593528456 54-55 0.3504363162687754 56-57 0.3282458587418875 58-59 0.3141246584975043 60-61 0.2737783720849808 62-63 0.3418867460527883 64-65 0.37003308395485823 66-67 0.3744999942362448 68-69 0.38660388016000186 70-71 0.3875645060269667 72-73 0.3706574907683854 74-75 0.3741157438894589 76-77 0.35759297897766357 78-79 0.3371796793046606 80-81 0.3340576452370248 82-83 0.3480347516013633 84-85 0.3549512578435102 86-87 0.3482268767747563 88-89 0.3634047654728008 90-91 0.3879007250804044 92-93 0.38401019031919675 94-95 0.38213696987861534 96-97 0.3868920679200913 98-99 0.3757488078632991 100-101 0.35456700749672426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1040988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.366949525534324 #Duplication Level Percentage of deduplicated Percentage of total 1 81.24388574110829 51.48177207015087 2 10.913986477397943 13.83172060471279 3 3.328792127040684 6.328062080855491 4 1.464345460275571 3.7116441947690966 5 0.8119072440346896 2.5724042676080923 6 0.4851467893426015 1.8445363267648593 7 0.3336068527788873 1.4797754020988476 8 0.24641898683088584 1.249185560051684 9 0.1799886562928396 1.02647988886294 >10 0.910914479799848 10.276742961956103 >50 0.05290306224268167 2.2614682296182007 >100 0.027040724242484916 3.0792723097848897 >500 6.076563500708601E-4 0.20706240811614154 >1k 4.5574226255314505E-4 0.6498736946499635 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2925 0.2809830660872171 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2315 0.2223848882023616 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.14515056849838806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.606258669648449E-5 2 0.0 0.0 0.0 0.0 9.606258669648449E-5 3 0.0 0.0 0.0 0.0 9.606258669648449E-5 4 0.0 0.0 0.0 0.0 9.606258669648449E-5 5 0.0 0.0 0.0 0.0 9.606258669648449E-5 6 0.0 0.0 0.0 0.0 9.606258669648449E-5 7 0.0 0.0 0.0 0.0 9.606258669648449E-5 8 0.0 0.0 0.0 0.0 9.606258669648449E-5 9 0.0 0.0 0.0 0.0 9.606258669648449E-5 10-11 0.0 0.0 0.0 0.0 9.606258669648449E-5 12-13 0.0 0.0 0.0 0.0 4.322816401341802E-4 14-15 0.0 0.0 0.0 0.0 5.76375520178907E-4 16-17 0.0 0.0 0.0 4.8031293348242244E-5 6.724381068753915E-4 18-19 0.0 0.0 0.0 9.606258669648449E-5 7.204694002236337E-4 20-21 0.0 0.0 0.0 9.606258669648449E-5 7.685006935718759E-4 22-23 0.0 0.0 0.0 9.606258669648449E-5 9.125945736166026E-4 24-25 0.0 0.0 0.0 3.8425034678593795E-4 9.60625866964845E-4 26-27 0.0 0.0 0.0 5.283442268306647E-4 0.001152751040357814 28-29 0.0 0.0 0.0 0.0014889700937955096 0.001152751040357814 30-31 0.0 0.0 0.0 0.004082659934600591 0.001152751040357814 32-33 0.0 0.0 0.0 0.01047082194991681 0.001152751040357814 34-35 0.0 0.0 0.0 0.023006989513808036 0.001152751040357814 36-37 0.0 0.0 0.0 0.04327619530676627 0.001152751040357814 38-39 0.0 0.0 0.0 0.07257528424919404 0.001152751040357814 40-41 0.0 0.0 0.0 0.10269090517854193 0.001152751040357814 42-43 0.0 0.0 0.0 0.1357364350021326 0.0012007823337060561 44-45 0.0 0.0 0.0 0.17925278677564005 0.0012488136270542983 46-47 0.0 0.0 0.0 0.23626593198000362 0.0012968449204025408 48-49 0.0 0.0 0.0 0.29769795617240546 0.0013929075070990252 50-51 0.0 0.0 0.0 0.3623480770191395 0.0015370013871437518 52-53 0.0 0.0 0.0 0.42469269578515795 0.0015370013871437518 54-55 0.0 0.0 0.0 0.5022632345425692 0.001585032680491994 56-57 0.0 0.0 0.0 0.5899683761964595 0.0016330639738402365 58-59 0.0 0.0 0.0 0.6979907549366564 0.0016330639738402365 60-61 0.0 0.0 0.0 0.8105761065449362 0.0016330639738402365 62-63 0.0 0.0 0.0 0.9349291250235354 0.0017291265605367209 64-65 0.0 0.0 0.0 1.060963238769323 0.0018251891472332053 66-67 0.0 0.0 0.0 1.2154318781772702 0.0018251891472332053 68-69 0.0 0.0 0.0 1.3908421614850508 0.0018251891472332053 70-71 0.0 0.0 0.0 1.5857531498922177 0.0018251891472332053 72-73 4.8031293348242244E-5 0.0 0.0 1.7914711793027394 0.001969283027277932 74-75 9.606258669648449E-5 0.0 0.0 2.000599430540986 0.0020173143206261746 76-77 9.606258669648449E-5 0.0 0.0 2.234367735266881 0.002161408200670901 78-79 9.606258669648449E-5 0.0 0.0 2.502814633790207 0.002257470787367386 80-81 9.606258669648449E-5 0.0 0.0 2.791146487759705 0.002305502080715628 82-83 9.606258669648449E-5 0.0 0.0 3.0952326059474267 0.002305502080715628 84-85 9.606258669648449E-5 0.0 0.0 3.4102698590185474 0.002305502080715628 86-87 9.606258669648449E-5 0.0 0.0 3.7474495383232087 0.002305502080715628 88-89 9.606258669648449E-5 0.0 0.0 4.121613313506016 0.002305502080715628 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2530 0.0 35.676533 1 GTATCAA 3815 0.0 35.4895 1 TATCAAC 4830 0.0 28.07581 2 ATCAACG 4815 0.0 27.966326 3 TCAACGC 4835 0.0 27.947365 4 GTACATG 4245 0.0 27.306227 1 CAACGCA 5000 0.0 27.0251 5 TACATGG 4255 0.0 26.521053 2 AACGCAG 5135 0.0 26.209673 6 ACATGGG 4435 0.0 24.803202 3 GAGTACT 2965 0.0 22.615982 12-13 ACGCAGA 5910 0.0 22.61233 7 CGCAGAG 6010 0.0 22.157232 8 CATGGGG 2530 0.0 21.738518 4 GTACTTT 3045 0.0 21.632723 14-15 GCAGAGT 6395 0.0 20.378672 9 GTCTATA 235 1.13006536E-7 20.215368 1 GTGTTAG 335 3.45608E-11 19.853302 1 GTGTAGC 455 0.0 19.837719 1 AGTACTT 3190 0.0 19.683798 12-13 >>END_MODULE