FastQCFastQC Report
Fri 27 May 2016
SRR939270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040988
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31230.3000034582531211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24440.23477696188620809No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.1746417826142088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.039.5943031
GTATCAA39500.033.2498321
TGCACCG2700.026.3629115
ATCAACG50650.025.7643453
TATCAAC51250.025.7404882
TCAACGC51450.025.3637354
GTACATG43200.025.2248861
CAACGCA52150.024.9322875
TACATGG43900.024.5373252
AACGCAG53800.024.1676356
TAGATTG1401.7928856E-523.726625
ACATGGG44800.023.0910853
GCACCGT2955.456968E-1222.5201846
ACGCAGA60150.021.3795997
GAGTACT31250.021.1092312-13
CGCAGAG61000.020.92618
TAGCGCG1150.003744349820.6318444
GTACTTT32100.020.5502614-15
AGTACTT33200.020.36968812-13
AGAGTAC55900.019.78068210-11
Produced by FastQC (version 0.11.5)