##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939270_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1040988 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27561028561328 33.0 31.0 34.0 31.0 34.0 2 32.4810084266101 34.0 31.0 34.0 31.0 34.0 3 32.57181158668496 34.0 31.0 34.0 31.0 34.0 4 36.10930577489846 37.0 35.0 37.0 35.0 37.0 5 36.04404949912967 37.0 35.0 37.0 35.0 37.0 6 36.07637552017891 37.0 35.0 37.0 35.0 37.0 7 36.01928264302759 37.0 35.0 37.0 35.0 37.0 8 35.99557535725676 37.0 35.0 37.0 35.0 37.0 9 37.70547883356965 39.0 37.0 39.0 35.0 39.0 10-11 37.6227334032669 39.0 37.0 39.0 35.0 39.0 12-13 37.54055041940926 39.0 37.0 39.0 35.0 39.0 14-15 38.84487333187318 40.0 38.0 41.0 35.0 41.0 16-17 38.780636280149245 40.0 38.0 41.0 35.0 41.0 18-19 38.882983281267414 40.0 38.0 41.0 35.0 41.0 20-21 38.881395847022254 40.0 38.0 41.0 35.0 41.0 22-23 38.824209789161834 40.0 38.0 41.0 34.5 41.0 24-25 38.750104708219496 40.0 38.0 41.0 34.0 41.0 26-27 38.582677706179126 40.0 38.0 41.0 34.0 41.0 28-29 38.4251595599565 40.0 38.0 41.0 34.0 41.0 30-31 38.239253478426264 40.0 38.0 41.0 34.0 41.0 32-33 38.04475796070656 40.0 37.5 41.0 33.0 41.0 34-35 38.00787569117031 40.0 37.0 41.0 33.0 41.0 36-37 37.91985066110272 40.0 37.0 41.0 33.0 41.0 38-39 37.81621017725469 40.0 37.0 41.0 33.0 41.0 40-41 37.63924319972949 40.0 37.0 41.0 33.0 41.0 42-43 37.457451959100396 40.0 37.0 41.0 32.0 41.0 44-45 37.23119286677657 40.0 36.5 41.0 31.5 41.0 46-47 36.94344075051778 40.0 36.0 41.0 31.0 41.0 48-49 36.62511575541697 39.5 36.0 41.0 30.5 41.0 50-51 36.36388219652868 39.0 35.0 41.0 30.0 41.0 52-53 36.10043679658171 39.0 35.0 40.5 29.5 41.0 54-55 35.791844382452055 38.5 35.0 40.0 29.0 41.0 56-57 35.51599970412723 38.0 35.0 40.0 28.0 41.0 58-59 35.18564094879096 38.0 34.0 40.0 28.0 41.0 60-61 34.82705324172805 37.5 34.0 40.0 27.0 41.0 62-63 34.417745449515266 37.0 34.0 40.0 26.0 41.0 64-65 33.964955407747254 36.0 33.0 39.0 26.0 41.0 66-67 33.56814535806369 36.0 33.0 39.0 26.0 40.0 68-69 33.16610325959569 35.0 33.0 38.5 25.0 40.0 70-71 32.74584529312538 35.0 32.0 37.5 24.5 40.0 72-73 32.24622906315923 35.0 32.0 37.0 22.5 39.0 74-75 31.747303042878496 35.0 31.0 36.5 21.5 39.0 76-77 29.802045268533355 33.0 28.5 35.0 19.0 37.0 78-79 30.78995531168467 34.0 30.5 35.0 20.0 37.0 80-81 30.851217785411553 34.0 31.0 35.0 20.0 37.0 82-83 30.56051990993172 34.0 31.0 35.0 18.5 36.5 84-85 30.1989998924099 34.0 30.5 35.0 16.5 36.0 86-87 29.66319832697399 34.0 30.0 35.0 8.5 36.0 88-89 29.17170178714836 34.0 29.0 35.0 4.0 35.0 90-91 28.74943178979969 34.0 29.0 35.0 2.0 35.0 92-93 28.384111536348165 34.0 29.0 35.0 2.0 35.0 94-95 27.969481396519463 33.0 28.0 35.0 2.0 35.0 96-97 27.395209166676274 33.0 27.0 35.0 2.0 35.0 98-99 26.607332649367716 33.0 25.5 35.0 2.0 35.0 100-101 23.182920936648646 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14756631604330295 1101 2 0.26102881557353896 1101 3 0.25625663650600927 1101 4 0.14669824834773948 1101 5 0.20065191454636988 1101 6 0.27244631194879787 1101 7 0.1746081004138489 1101 8 0.16613689315386893 1101 9 0.2232976243509981 1101 10-11 0.30645289621995175 1101 12-13 0.3631246376918327 1101 14-15 0.33631310238838097 1101 16-17 0.37889082166629606 1101 18-19 0.3603555454805232 1101 20-21 0.4389349106815601 1101 22-23 0.37157416858945425 1101 24-25 0.3594623883479926 1101 26-27 0.26991968088021423 1101 28-29 0.39865810142774194 1101 30-31 0.5014407030438051 1101 32-33 0.3339373612003058 1101 34-35 0.3362000867457624 1101 36-37 0.5650525993159192 1101 38-39 0.561366392577483 1101 40-41 0.5283642723893607 1101 42-43 0.5324278010119983 1101 44-45 0.6545863416250697 1101 46-47 0.7486848052322514 1101 48-49 0.7250889643299843 1101 50-51 0.8524103292464176 1101 52-53 0.7564441442935745 1101 54-55 0.7301316522514583 1101 56-57 0.6918999299937809 1101 58-59 0.5345186063602583 1101 60-61 0.6487279877132366 1101 62-63 0.7060522111938354 1101 64-65 0.6260150980146193 1101 66-67 0.8409442575572328 1101 68-69 0.7942191626043282 1101 70-71 0.8409839113827005 1101 72-73 0.6525174955272703 1101 74-75 0.591090322495905 1101 76-77 0.6948174983947695 1101 78-79 0.9762539657528713 1101 80-81 0.9861915380141824 1101 82-83 1.3071258102318417 1101 84-85 1.1924144464279074 1101 86-87 1.3406724449238396 1101 88-89 1.58212304984189 1101 90-91 1.4351259373807856 1101 92-93 1.366625100542791 1101 94-95 1.570370308771306 1101 96-97 1.4127974046106644 1101 98-99 1.0937522378111026 1101 100-101 0.7090101716594184 1102 1 0.2551106611868832 1102 2 0.12297138237479288 1102 3 0.17169464403674084 1102 4 0.12061513806330026 1102 5 0.04874654776326537 1102 6 -0.04235203673332677 1102 7 0.15478321269439732 1102 8 0.20381778958357444 1102 9 0.3638978148876788 1102 10-11 0.22584113738494693 1102 12-13 0.3181681889325958 1102 14-15 0.21361655473153007 1102 16-17 0.3083831775162267 1102 18-19 0.28522511858762556 1102 20-21 0.24138580212102312 1102 22-23 0.21852898417716915 1102 24-25 0.28884926853671544 1102 26-27 0.3885196899533909 1102 28-29 0.17231867394509948 1102 30-31 0.09314328436214225 1102 32-33 0.13491386167664388 1102 34-35 0.41005233008831965 1102 36-37 0.3588147913950408 1102 38-39 0.1848602455013264 1102 40-41 0.3102485440402205 1102 42-43 0.35819902090039335 1102 44-45 0.34685713053765 1102 46-47 0.6041888201122632 1102 48-49 0.22806950236926582 1102 50-51 0.2927111050029012 1102 52-53 0.2962311515067455 1102 54-55 0.2617448627944867 1102 56-57 0.4696525045072306 1102 58-59 0.32671113913711025 1102 60-61 0.2991430854766932 1102 62-63 0.0950589253434444 1102 64-65 0.23827409179949655 1102 66-67 0.0525454461431778 1102 68-69 0.36837308902087784 1102 70-71 0.3727649756661435 1102 72-73 0.4914731730666233 1102 74-75 0.3542294142710709 1102 76-77 0.4870599336349848 1102 78-79 0.42685846604774724 1102 80-81 0.5462060550944337 1102 82-83 0.42106901215535686 1102 84-85 0.24458066699679648 1102 86-87 0.2885992244003148 1102 88-89 0.4773119987145513 1102 90-91 0.6910654646683589 1102 92-93 0.6935701624672106 1102 94-95 0.833430010263843 1102 96-97 0.4892865394834125 1102 98-99 0.2686569694719445 1102 100-101 0.09282022473749052 1103 1 0.1288926608218759 1103 2 0.16549734486772394 1103 3 0.1350377851756619 1103 4 0.09888365233654639 1103 5 0.0653545308125274 1103 6 0.047527376222774365 1103 7 0.08730298781224377 1103 8 0.16289876407289228 1103 9 0.09704834615617841 1103 10-11 0.11597739317726052 1103 12-13 0.1768222790546048 1103 14-15 0.24686034176118454 1103 16-17 0.10042060445846346 1103 18-19 0.175857699791365 1103 20-21 0.1588822744269507 1103 22-23 0.1299326725649408 1103 24-25 0.25519929591251156 1103 26-27 0.2895216886090921 1103 28-29 -0.014434334053980535 1103 30-31 0.13958977247035875 1103 32-33 0.06322377349542307 1103 34-35 0.16452927432627007 1103 36-37 0.25770351212185005 1103 38-39 0.3614341312173863 1103 40-41 0.4044947303469826 1103 42-43 0.3156630428722522 1103 44-45 0.4039078447565956 1103 46-47 0.26208261982826997 1103 48-49 0.2572792543846134 1103 50-51 0.35604378547171933 1103 52-53 0.38235028741087973 1103 54-55 0.13213716018021415 1103 56-57 0.3252117942499879 1103 58-59 0.12356937702609372 1103 60-61 0.08108262725483684 1103 62-63 0.1267258219964802 1103 64-65 0.19418793148861369 1103 66-67 0.23829578778421734 1103 68-69 0.1256310135348997 1103 70-71 0.16478793572418482 1103 72-73 0.4051648548307867 1103 74-75 0.5504637956003258 1103 76-77 0.8027395878541945 1103 78-79 0.5247892564737846 1103 80-81 0.4046231108831897 1103 82-83 0.5650501206302785 1103 84-85 0.6006741186173095 1103 86-87 0.498921012418176 1103 88-89 0.8053118740767786 1103 90-91 0.6160847167527059 1103 92-93 0.7793910579895993 1103 94-95 0.6076876365374844 1103 96-97 0.08010296140007611 1103 98-99 0.10727442499184292 1103 100-101 0.09666730309458771 1104 1 0.11368020840875914 1104 2 0.1622759101560547 1104 3 0.14587312139783393 1104 4 0.07505512301250405 1104 5 0.06401683498263111 1104 6 0.12085503714099843 1104 7 0.1342451467217387 1104 8 0.2091326020757336 1104 9 0.11689229626232134 1104 10-11 0.08027955454285518 1104 12-13 0.13835036628114494 1104 14-15 0.16513260891439785 1104 16-17 0.1381523986375086 1104 18-19 0.004613993540594663 1104 20-21 0.1236054810373659 1104 22-23 0.1487152377830867 1104 24-25 -0.01615668907020762 1104 26-27 0.26941458522493633 1104 28-29 0.24302606137767668 1104 30-31 0.18673258575434204 1104 32-33 0.15547544451873563 1104 34-35 0.2126282111870239 1104 36-37 0.12829459824315137 1104 38-39 0.2241569343261034 1104 40-41 0.29732994376429644 1104 42-43 0.3224063456213244 1104 44-45 0.47170819036456635 1104 46-47 0.20007493575254642 1104 48-49 0.2489603213834357 1104 50-51 0.008384314585235586 1104 52-53 -0.13160341515573748 1104 54-55 -0.03921619326973058 1104 56-57 0.264177777302919 1104 58-59 0.16271233065849344 1104 60-61 0.3556227669015115 1104 62-63 0.22408814897320184 1104 64-65 0.10784953391664942 1104 66-67 0.2055540376394802 1104 68-69 0.20825456399892772 1104 70-71 0.08482442631207476 1104 72-73 0.07253582200679887 1104 74-75 0.05700754044691436 1104 76-77 0.2544389001469618 1104 78-79 0.362806352665082 1104 80-81 0.45650487611537827 1104 82-83 0.490138057684014 1104 84-85 0.323095655081584 1104 86-87 0.11332799650617531 1104 88-89 0.37926233108630214 1104 90-91 0.422071673121895 1104 92-93 0.6249792932837153 1104 94-95 0.5824501407040792 1104 96-97 0.11449819130832495 1104 98-99 0.2561851897152678 1104 100-101 0.4095532612356756 1105 1 0.054592184710067215 1105 2 0.09372654796513302 1105 3 0.14450530808546347 1105 4 0.13353103136144995 1105 5 0.16112402318164953 1105 6 0.18214214191096545 1105 7 0.2304454973655936 1105 8 0.11712637512452773 1105 9 0.06803642297775525 1105 10-11 0.2020111608544397 1105 12-13 0.05047402869575279 1105 14-15 0.13150782806680894 1105 16-17 0.1574075827255328 1105 18-19 0.2018700542382632 1105 20-21 0.1860752531188652 1105 22-23 0.2021808686397506 1105 24-25 0.16791999915903943 1105 26-27 0.08870453141924628 1105 28-29 0.17209625060445433 1105 30-31 0.12371115416011946 1105 32-33 0.09194013830992986 1105 34-35 0.2830025839577175 1105 36-37 0.1551785532640011 1105 38-39 0.21777506503162414 1105 40-41 0.20953295446497577 1105 42-43 0.27270409061860335 1105 44-45 0.4335945636189038 1105 46-47 0.3497787021511911 1105 48-49 0.2968980216434005 1105 50-51 0.3906579074756351 1105 52-53 0.502041769536838 1105 54-55 0.4819017862961985 1105 56-57 0.3031264502972917 1105 58-59 0.24262361171767566 1105 60-61 0.05728454672135541 1105 62-63 -0.06466404255089486 1105 64-65 -0.02705010939391883 1105 66-67 0.08791075730576381 1105 68-69 0.09387967735361968 1105 70-71 0.12862468022658646 1105 72-73 0.023003599045665624 1105 74-75 0.24309060676696603 1105 76-77 0.3484288342501003 1105 78-79 0.3816950232412246 1105 80-81 0.40695753919500177 1105 82-83 0.3924300896046944 1105 84-85 0.28246933261014817 1105 86-87 0.33546919111351414 1105 88-89 0.4584443636115445 1105 90-91 0.6212245357057746 1105 92-93 0.6758453231488986 1105 94-95 0.5121740086685342 1105 96-97 0.4777281955402337 1105 98-99 0.42443000562724364 1105 100-101 0.5633491321020045 1106 1 0.01047326297998552 1106 2 0.046109564099559464 1106 3 0.07848170700075485 1106 4 0.12064082717368763 1106 5 -0.023602550120806143 1106 6 -0.017851047702571066 1106 7 0.030713372566289365 1106 8 -0.07849268900454831 1106 9 0.039840391162925926 1106 10-11 0.09880955690524473 1106 12-13 0.06466697726288118 1106 14-15 0.06372487714698849 1106 16-17 -0.03344575869770949 1106 18-19 -0.057088699584845415 1106 20-21 -0.060131567999114566 1106 22-23 0.08450461377444896 1106 24-25 0.085951322809251 1106 26-27 0.04510980307110657 1106 28-29 0.11259256267699413 1106 30-31 -0.008575959350451967 1106 32-33 0.1729465896032778 1106 34-35 0.0027108104918056597 1106 36-37 -0.01815042554949997 1106 38-39 0.08752848776676103 1106 40-41 0.13337580789307424 1106 42-43 0.13126784202712116 1106 44-45 0.3616144540748749 1106 46-47 0.3872817461875613 1106 48-49 0.10934513796067336 1106 50-51 0.17865573640698074 1106 52-53 0.1726429821983828 1106 54-55 0.1645800842774392 1106 56-57 0.2361834470406876 1106 58-59 0.148329167754774 1106 60-61 0.10053308042450482 1106 62-63 0.20065829510394195 1106 64-65 0.2581735189323382 1106 66-67 0.18231612801779562 1106 68-69 0.15307586601371526 1106 70-71 0.25343584678388 1106 72-73 0.2691521191280515 1106 74-75 0.21901692251299565 1106 76-77 0.3032960936267628 1106 78-79 0.3857152150051988 1106 80-81 0.32783684263693047 1106 82-83 0.08764362617647237 1106 84-85 0.15968993134630338 1106 86-87 0.24494058129912588 1106 88-89 0.22143775547292677 1106 90-91 0.3271984828819434 1106 92-93 0.2397324234514464 1106 94-95 0.4032016340725093 1106 96-97 0.1277465629461325 1106 98-99 0.1412443338548499 1106 100-101 0.20761736639580874 1107 1 0.01597455810129844 1107 2 -0.0018693337403234978 1107 3 0.08447015784555134 1107 4 0.06803989088415108 1107 5 0.07346025732198314 1107 6 0.16040636062897562 1107 7 0.12829898127331063 1107 8 0.1377806885292543 1107 9 0.04610806541021617 1107 10-11 0.12963997918608072 1107 12-13 0.17107188684202868 1107 14-15 0.20890322366917502 1107 16-17 0.15111793988359778 1107 18-19 0.13843897323661736 1107 20-21 0.15307629303080006 1107 22-23 0.06445993803005479 1107 24-25 0.2033469407841011 1107 26-27 0.3186061553441064 1107 28-29 0.30332888073027675 1107 30-31 0.34401487736920444 1107 32-33 0.25140242477829844 1107 34-35 0.19851176579230412 1107 36-37 0.3078428968525273 1107 38-39 0.30941603796756567 1107 40-41 0.21911818568828068 1107 42-43 0.3256073301571476 1107 44-45 0.3025646453726054 1107 46-47 0.3323905839053012 1107 48-49 0.4519700755809737 1107 50-51 0.35282578940002196 1107 52-53 0.45186044376060863 1107 54-55 0.298999418418731 1107 56-57 0.10888133920104792 1107 58-59 0.013972213309777715 1107 60-61 -0.03549921443540427 1107 62-63 -0.15525648828998584 1107 64-65 0.18414070127116133 1107 66-67 0.1345035760347244 1107 68-69 0.295120375412246 1107 70-71 0.1600237368055062 1107 72-73 0.13052958164082895 1107 74-75 0.35932965272377615 1107 76-77 0.32986093286537255 1107 78-79 0.3818952012222532 1107 80-81 0.25950137057115086 1107 82-83 0.3238785065262739 1107 84-85 0.434543784629259 1107 86-87 0.4498964996470747 1107 88-89 0.46828906604064 1107 90-91 0.6332154501593337 1107 92-93 0.46578159053397883 1107 94-95 0.5346208018679555 1107 96-97 0.5896890072796843 1107 98-99 0.41747648137986104 1107 100-101 0.2415430097978195 1108 1 -0.010654559184516188 1108 2 0.06530666047350309 1108 3 0.14056642701652322 1108 4 0.09815627877798505 1108 5 0.10211301666993933 1108 6 0.12268897572124615 1108 7 0.13032452168635444 1108 8 0.008233426236003538 1108 9 0.10471490364983538 1108 10-11 0.21226095634322917 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-0.802813658374923 2316 46-47 -0.8462981734911565 2316 48-49 -0.8263833760955492 2316 50-51 -0.5822535005408085 2316 52-53 -0.7312622386851544 2316 54-55 -0.6820640924293926 2316 56-57 -0.6288575168147332 2316 58-59 -0.7413792659801643 2316 60-61 -0.6779528633505905 2316 62-63 -0.5932967249763763 2316 64-65 -0.7182529870917591 2316 66-67 -0.7052131892512747 2316 68-69 -0.5463723267573712 2316 70-71 -0.5443352375534758 2316 72-73 -0.4232952704301809 2316 74-75 -0.3659437200572917 2316 76-77 -0.26368462926480163 2316 78-79 -0.3684566725450047 2316 80-81 -0.5386169726241157 2316 82-83 -0.5462358918958188 2316 84-85 -0.6139430003806012 2316 86-87 -0.7461871295442464 2316 88-89 -0.7946939714347039 2316 90-91 -0.9293123604915543 2316 92-93 -0.8550089420104001 2316 94-95 -0.6918296912286941 2316 96-97 -0.271806850708483 2316 98-99 -0.1765669111250716 2316 100-101 0.0400952780423971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 9.0 5 24.0 6 42.0 7 121.0 8 303.0 9 571.0 10 915.0 11 1450.0 12 2019.0 13 2673.0 14 3446.0 15 4530.0 16 6003.0 17 8137.0 18 7883.0 19 5864.0 20 5655.0 21 6105.0 22 6315.0 23 6853.0 24 8224.0 25 9630.0 26 11636.0 27 14052.0 28 16987.0 29 20491.0 30 25469.0 31 30830.0 32 37906.0 33 49025.0 34 66538.0 35 96710.0 36 153126.0 37 239356.0 38 178409.0 39 13678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31097783009689 18.620926831805825 14.859826948753172 23.208268389344113 2 16.16358690013718 19.997636860367265 38.82033222284983 25.018444016645724 3 19.82155413895261 23.47443006067313 29.045867963895837 27.658147836478424 4 13.982581931780194 16.273290374144565 35.00760815686636 34.736519537208885 5 14.917943338443862 36.55114179990547 32.94235860547864 15.588556256172021 6 33.789438494968245 34.754387178334426 16.508259461204165 14.947914865493168 7 30.197466253213296 31.772892675035642 19.428466034190595 18.601175037560473 8 27.279084869374092 34.750352549693176 18.651607895576124 19.318954685356605 9 26.703669975062148 16.974643319615595 19.368715105265384 36.95297160005687 10-11 25.98800370417334 26.0602427693691 25.94002044211845 22.01173308433911 12-13 26.694063716392503 23.00141788377964 26.95319254400627 23.351325855821585 14-15 23.46987935603395 24.596536547431665 25.003182172532835 26.930401924001558 16-17 23.350282089682715 27.845128777566547 25.608872784421617 23.195716348329125 18-19 23.340374720938186 26.111684284545067 27.898016115459544 22.649924879057203 20-21 23.927365156947054 25.33847652422506 27.923040419293976 22.811117899533905 22-23 23.696094479475267 25.089050017867642 28.002676303665364 23.212179198991727 24-25 23.579437793441326 25.422447435728095 27.93539787941738 23.062716891413203 26-27 23.306483376081925 25.7050635464999 27.812809206797507 23.17564387062067 28-29 23.22077680050106 25.792564371539346 27.972560682736013 23.014098145223578 30-31 23.239845223960316 25.58968018843637 27.83557543410683 23.334899153496487 32-33 23.105357602585237 25.677817611730397 27.77020484385987 23.446619941824498 34-35 23.693397531471433 25.674916229883642 27.53202862017553 23.099657618469386 36-37 23.8034750173674 25.40881850754701 27.498287281298072 23.289419193787513 38-39 23.443326552326237 25.583498855554943 27.63831212967655 23.334862462442267 40-41 23.477208144872463 25.524139405396447 27.728260843455978 23.27039160627512 42-43 23.499596510778925 25.558352226876224 27.357481842985052 23.584569419359795 44-45 23.428194584603975 25.712972386984724 27.343268556014472 23.51556447239683 46-47 23.6571385205949 25.950204901891095 27.003052874870555 23.389603702643456 48-49 23.78817046882385 25.95399754848279 26.71106679423778 23.54676518845558 50-51 23.655940318236137 26.14953294370348 26.692718840178753 23.501807897881626 52-53 23.961179187464214 26.01398863387474 26.572928794568234 23.451903384092805 54-55 23.531683362344236 25.98920448650705 26.687771616963886 23.791340534184833 56-57 23.606227929620708 26.211685437296104 26.606310543445268 23.57577608963792 58-59 23.682839095294383 25.877070585924123 26.70528093973032 23.73480937905117 60-61 23.63336027970429 25.9356658995837 26.94473784447285 23.48623597623916 62-63 23.408803043701088 26.101917491338604 26.929127126203024 23.560152338757284 64-65 23.32654170845389 25.920807924779155 26.844545758452547 23.90810460831441 66-67 23.289269424815657 26.147803817142943 26.7555918031716 23.807334954869795 68-69 23.296811710870944 25.950028891003345 26.97752073403802 23.77563866408769 70-71 23.366839963573067 25.99568871110906 26.760442963799775 23.877028361518096 72-73 23.43638927634132 26.05332626312695 26.64684895503118 23.863435505500544 74-75 23.332113338482287 26.39386813296599 26.334597516974256 23.939421011577462 76-77 23.471967984870616 26.045802202965984 26.576129816881704 23.906099995281696 78-79 23.357858111716943 26.140983373487494 26.593486188121286 23.907672326674277 80-81 23.341236211191283 26.398862216142426 26.518894378421688 23.741007194244602 82-83 23.456562419547584 26.259572636764304 26.471294577843356 23.812570365844753 84-85 23.335715685483407 26.199533520079 26.369660361118473 24.095090433319115 86-87 23.406914246532924 26.113114065161135 26.516776890579724 23.963194797726217 88-89 23.197065378631816 26.13303661864819 26.420077222310873 24.249820780409117 90-91 23.204054989577127 26.218492742555988 26.488899302120632 24.088552965746253 92-93 23.253197923511127 25.985986389852716 26.525425845446826 24.23538984118933 94-95 23.120967772923414 25.764370002343927 26.65900087224829 24.45566135248437 96-97 23.249355420043265 25.700056100550633 26.646945017617877 24.403643461788224 98-99 22.991139187003114 25.843909824128612 26.439353767766775 24.72559722110149 100-101 23.01417552326668 25.3824624670204 27.0152437428223 24.58811826689062 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 178.5 2 146.0 3 181.0 4 149.0 5 149.0 6 253.0 7 565.0 8 944.5 9 1077.0 10 961.5 11 834.0 12 844.5 13 944.0 14 1031.0 15 1096.5 16 1126.0 17 1166.5 18 1232.0 19 1285.5 20 1403.5 21 1572.0 22 1854.0 23 2242.5 24 2591.0 25 3104.5 26 3923.5 27 5002.5 28 5875.5 29 6883.5 30 8399.0 31 10026.0 32 11820.5 33 13078.5 34 14095.5 35 15422.0 36 17347.0 37 19639.0 38 21759.5 39 23092.5 40 24232.0 41 26650.5 42 29003.5 43 30908.0 44 33053.0 45 35471.0 46 36537.0 47 37630.0 48 41389.5 49 43275.0 50 42158.0 51 41445.5 52 41628.0 53 42480.5 54 43257.0 55 41594.0 56 37860.0 57 34409.0 58 31856.5 59 28548.0 60 23979.0 61 19970.0 62 16734.0 63 13138.0 64 9792.0 65 7500.0 66 5679.0 67 4502.0 68 3324.5 69 2295.0 70 1755.5 71 1317.0 72 1036.5 73 872.5 74 711.5 75 574.5 76 403.0 77 231.0 78 144.5 79 95.0 80 50.0 81 33.5 82 21.5 83 9.5 84 5.5 85 2.5 86 2.0 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2799263776335558 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.003122034067635746 16-17 6.724381068753914E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.8031293348242244E-5 26-27 0.0017291265605367209 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.004226753814645317 36-37 0.0235353337406387 38-39 0.008693664096031846 40-41 0.020221174499609987 42-43 0.006532255895360946 44-45 0.0011047197470095717 46-47 1.9212517339296898E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.165319869201182E-4 60-61 0.0030259714809392616 62-63 0.0017771578538849633 64-65 0.0 66-67 0.0 68-69 6.244068135271492E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23813915242058506 78-79 0.0 80-81 0.0016330639738402365 82-83 0.0 84-85 0.0 86-87 0.1090310359005099 88-89 0.3700811152482065 90-91 0.36945670843467937 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.362190534376957E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1040988.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.69510785840395 #Duplication Level Percentage of deduplicated Percentage of total 1 79.98967841634762 49.349718374556126 2 11.642563512235876 14.365784232714212 3 3.5501984820335974 6.570896348034141 4 1.5395389101217734 3.7992807644868987 5 0.8564521059043219 2.6419452524662166 6 0.5311360732324834 1.9661098395340322 7 0.37256176538200375 1.6089666809412075 8 0.2539085820325902 1.2531933883740045 9 0.1886418789907592 1.047445296385219 >10 0.9886058449429382 10.813934373257304 >50 0.055278675991929066 2.3045505375931015 >100 0.029568283343392076 3.0872490039736706 >500 0.0014006020806080705 0.4818814197569623 >1k 4.668673602026902E-4 0.7090444879269111 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3123 0.3000034582531211 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2444 0.23477696188620809 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1818 0.1746417826142088 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.606258669648449E-5 7 0.0 0.0 0.0 0.0 9.606258669648449E-5 8 0.0 0.0 0.0 0.0 9.606258669648449E-5 9 0.0 0.0 0.0 0.0 9.606258669648449E-5 10-11 0.0 0.0 0.0 0.0 9.606258669648449E-5 12-13 0.0 0.0 0.0 0.0 4.322816401341802E-4 14-15 0.0 0.0 0.0 4.8031293348242244E-5 5.283442268306647E-4 16-17 0.0 0.0 0.0 1.4409388004472674E-4 5.76375520178907E-4 18-19 0.0 0.0 0.0 1.9212517339296898E-4 5.76375520178907E-4 20-21 0.0 0.0 0.0 1.9212517339296898E-4 6.724381068753915E-4 22-23 0.0 0.0 0.0 1.9212517339296898E-4 6.724381068753915E-4 24-25 0.0 0.0 0.0 4.803129334824225E-4 6.724381068753915E-4 26-27 0.0 0.0 0.0 6.244068135271492E-4 6.724381068753915E-4 28-29 0.0 0.0 0.0 0.0015850326804919942 6.724381068753915E-4 30-31 0.0 0.0 0.0 0.004226753814645318 6.724381068753915E-4 32-33 0.0 0.0 0.0 0.010518853243265052 7.685006935718759E-4 34-35 0.0 0.0 0.0 0.022958958220459794 7.685006935718759E-4 36-37 0.0 0.0 0.0 0.043660445653552205 7.685006935718759E-4 38-39 0.0 0.0 0.0 0.07348787882281063 7.685006935718759E-4 40-41 0.0 0.0 0.0 0.10393971880559622 7.685006935718759E-4 42-43 0.0 0.0 0.0 0.13679312345579392 7.685006935718759E-4 44-45 0.0 0.0 0.0 0.1803094752293014 7.685006935718759E-4 46-47 0.0 0.0 0.0 0.23713049526027197 7.685006935718759E-4 48-49 0.0 0.0 0.0 0.29885070721276324 0.0010566884536613295 50-51 0.0 0.0 0.0 0.36345279676614906 0.001152751040357814 52-53 0.0 0.0 0.0 0.4256052903587746 0.001152751040357814 54-55 0.0 0.0 0.0 0.5038962985164095 0.001152751040357814 56-57 0.0 0.0 0.0 0.5922738782771751 0.001152751040357814 58-59 0.0 0.0 0.0 0.7006805073641579 0.001152751040357814 60-61 0.0 0.0 0.0 0.8137942031992684 0.0014409388004472674 62-63 0.0 0.0 0.0 0.9386755659046981 0.0015370013871437518 64-65 0.0 0.0 0.0 1.0646136170637894 0.0015370013871437518 66-67 0.0 0.0 0.0 1.2198507571653083 0.0015370013871437518 68-69 0.0 0.0 0.0 1.3957413534065715 0.0015370013871437518 70-71 0.0 0.0 0.0 1.5904602166403454 0.0015370013871437518 72-73 0.0 0.0 0.0 1.7967546215710462 0.001585032680491994 74-75 0.0 0.0 0.0 2.0063631857427753 0.0016810952671884787 76-77 0.0 0.0 0.0 2.240227553055367 0.0017291265605367209 78-79 0.0 0.0 0.0 2.509539014858961 0.0017291265605367209 80-81 0.0 0.0 0.0 2.7998881831490854 0.0017291265605367209 82-83 0.0 0.0 0.0 3.1050790210838164 0.0018251891472332053 84-85 0.0 0.0 0.0 3.4207406809684646 0.0018251891472332053 86-87 0.0 0.0 0.0 3.7601778310604925 0.00192125173392969 88-89 0.0 0.0 0.0 4.1343416062433 0.0020173143206261746 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 39.594303 1 GTATCAA 3950 0.0 33.249832 1 TGCACCG 270 0.0 26.362911 5 ATCAACG 5065 0.0 25.764345 3 TATCAAC 5125 0.0 25.740488 2 TCAACGC 5145 0.0 25.363735 4 GTACATG 4320 0.0 25.224886 1 CAACGCA 5215 0.0 24.932287 5 TACATGG 4390 0.0 24.537325 2 AACGCAG 5380 0.0 24.167635 6 TAGATTG 140 1.7928856E-5 23.72662 5 ACATGGG 4480 0.0 23.091085 3 GCACCGT 295 5.456968E-12 22.520184 6 ACGCAGA 6015 0.0 21.379599 7 GAGTACT 3125 0.0 21.10923 12-13 CGCAGAG 6100 0.0 20.9261 8 TAGCGCG 115 0.0037443498 20.631844 4 GTACTTT 3210 0.0 20.55026 14-15 AGTACTT 3320 0.0 20.369688 12-13 AGAGTAC 5590 0.0 19.780682 10-11 >>END_MODULE