FastQCFastQC Report
Fri 27 May 2016
SRR939269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916014
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81260.2786680722383363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59080.20260533728576063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.141460226185471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80650.033.537731
GTATCAA123200.032.662421
GTACATG137550.026.3571
ATCAACG152850.025.9316863
TACATGG139500.025.592382
TCAACGC154900.025.5578864
TATCAAC157750.025.3666442
CAACGCA157400.025.1519495
AACGCAG161750.024.489696
ACATGGG140850.024.4382253
CATGGGG61300.022.9713154
ACGCAGA181450.021.621947
CGCAGAG184300.021.2622368
GAGTACT85850.020.61479812-13
GCAGAGT196250.019.5808939
GTGTAGC14650.019.4346541
AGTACTT92800.019.2496212-13
GTACTTT93100.018.85919814-15
CATGGGA79050.018.6529884
GATTACG1559.693645E-418.3688851
Produced by FastQC (version 0.11.5)