FastQCFastQC Report
Fri 27 May 2016
SRR939269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916014
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85020.291562386188818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62940.2158425851179041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46530.15956713513721127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80850.034.3230251
GTATCAA124300.033.315411
ATCAACG155500.026.5209313
TCAACGC156200.026.3716954
CAACGCA158600.026.0025525
TATCAAC160750.025.6547722
AACGCAG163550.025.2735926
GTACATG128850.025.2309341
TACATGG133250.024.253772
ACATGGG134400.023.1634883
ACGCAGA185700.022.1567767
CGCAGAG188150.021.8430398
GAGTACT94300.021.1370512-13
TAAGGTG12400.021.0494335
GTATAGG9250.020.5833131
AGTACTT99800.020.49526612-13
GCAGAGT202500.020.0373579
GTACTTT102000.019.61120414-15
CATGGGG58950.019.401384
CATGGGA74850.019.0208024
Produced by FastQC (version 0.11.5)