##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939269_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2916014 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3187663022194 33.0 31.0 34.0 31.0 34.0 2 32.52739355846714 34.0 31.0 34.0 31.0 34.0 3 32.61802275297718 34.0 31.0 34.0 31.0 34.0 4 36.14275857386144 37.0 35.0 37.0 35.0 37.0 5 36.083956044106785 37.0 35.0 37.0 35.0 37.0 6 36.11190824186715 37.0 35.0 37.0 35.0 37.0 7 36.05220516773925 37.0 35.0 37.0 35.0 37.0 8 36.024172723450576 37.0 35.0 37.0 35.0 37.0 9 37.73097214210906 39.0 38.0 39.0 35.0 39.0 10-11 37.655602647998265 39.0 37.0 39.0 35.0 39.0 12-13 37.57895932598403 39.0 37.0 39.0 35.0 39.0 14-15 38.88421026099326 40.0 38.0 41.0 35.0 41.0 16-17 38.88533885639781 40.0 38.0 41.0 35.0 41.0 18-19 38.97911841301173 40.0 38.5 41.0 35.0 41.0 20-21 38.95731690588591 40.0 38.0 41.0 35.0 41.0 22-23 38.88189494289122 40.0 38.0 41.0 35.0 41.0 24-25 38.812716262679125 40.0 38.0 41.0 34.5 41.0 26-27 38.66427767493572 40.0 38.0 41.0 34.0 41.0 28-29 38.49884619895515 40.0 38.0 41.0 34.0 41.0 30-31 38.29998432792161 40.0 38.0 41.0 34.0 41.0 32-33 38.25842177026585 40.0 38.0 41.0 34.0 41.0 34-35 38.16590506767114 40.0 38.0 41.0 33.5 41.0 36-37 38.013483131425296 40.0 38.0 41.0 33.0 41.0 38-39 37.88250570813446 40.0 37.0 41.0 33.0 41.0 40-41 37.691300864810664 40.0 37.0 41.0 33.0 41.0 42-43 37.503586059600536 40.0 37.0 41.0 32.5 41.0 44-45 37.266382465927805 40.0 37.0 41.0 32.0 41.0 46-47 36.96779285010291 40.0 36.0 41.0 31.0 41.0 48-49 36.66580235897358 39.5 36.0 41.0 30.5 41.0 50-51 36.41395188774814 39.0 35.5 41.0 30.0 41.0 52-53 36.15484304945038 39.0 35.0 40.5 30.0 41.0 54-55 35.90453921003123 39.0 35.0 40.0 29.0 41.0 56-57 35.59768986019957 38.0 35.0 40.0 28.0 41.0 58-59 35.282627586836 38.0 34.5 40.0 28.0 41.0 60-61 34.94590303750256 38.0 34.0 40.0 27.5 41.0 62-63 34.60213068250015 37.0 34.0 40.0 26.5 41.0 64-65 34.29841351927666 37.0 34.0 39.5 26.0 41.0 66-67 33.889137706471914 36.0 33.0 39.0 26.0 40.5 68-69 33.42949862380634 36.0 33.0 39.0 25.5 40.0 70-71 32.95372964601678 35.0 32.0 38.0 24.5 40.0 72-73 32.47553183901037 35.0 32.0 37.5 23.5 39.5 74-75 31.95669979636586 35.0 31.0 37.0 22.0 39.0 76-77 29.993900577980764 33.0 29.0 35.0 19.0 37.5 78-79 30.96662361703339 34.0 30.5 36.0 20.0 38.0 80-81 30.978306859980783 34.0 31.0 35.5 20.0 37.0 82-83 30.633537081783558 34.0 31.0 35.0 18.0 37.0 84-85 30.194453627451722 34.0 30.5 35.0 16.0 36.5 86-87 29.596772169132247 34.0 29.5 35.0 7.5 36.0 88-89 29.012849561078923 34.0 29.0 35.0 2.0 35.5 90-91 28.53801490664997 33.5 29.0 35.0 2.0 35.0 92-93 28.185537689462397 33.0 29.0 35.0 2.0 35.0 94-95 27.667422035696674 33.0 27.0 35.0 2.0 35.0 96-97 26.940962732003342 33.0 26.0 34.5 2.0 35.0 98-99 25.943466320806415 32.5 24.5 34.0 2.0 35.0 100-101 21.582145010277728 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23399921550458913 1101 2 0.17985533457445513 1101 3 0.17780444512980864 1101 4 0.134319810827904 1101 5 0.155616028515432 1101 6 0.16546515327218714 1101 7 0.22279773968072192 1101 8 0.16950409939455824 1101 9 0.19876797683814118 1101 10-11 0.23403787589226965 1101 12-13 0.20131754789107958 1101 14-15 0.20692932150537757 1101 16-17 0.2686943612077215 1101 18-19 0.2241506346763984 1101 20-21 0.2181851574547906 1101 22-23 0.25206822905992254 1101 24-25 0.17894094173613695 1101 26-27 0.2049120499152508 1101 28-29 0.26090920591722266 1101 30-31 0.31003338931281377 1101 32-33 0.33019738555386624 1101 34-35 0.4100893372312484 1101 36-37 0.45258989735231836 1101 38-39 0.4670772437094257 1101 40-41 0.4872234198875276 1101 42-43 0.3807704805954941 1101 44-45 0.49616291324270634 1101 46-47 0.4451907518668534 1101 48-49 0.3493920858791384 1101 50-51 0.26452486733042946 1101 52-53 0.3345421632891643 1101 54-55 0.44047532362106523 1101 56-57 0.3743546755760647 1101 58-59 0.25713247136104656 1101 60-61 0.30553791603647795 1101 62-63 0.2725094552407441 1101 64-65 0.26811587309654783 1101 66-67 0.22538604911684956 1101 68-69 0.363669984825691 1101 70-71 0.3681428569014429 1101 72-73 0.33409577047194006 1101 74-75 0.4262150006227472 1101 76-77 0.5099449786861854 1101 78-79 0.5049988119426452 1101 80-81 0.6113271928233992 1101 82-83 0.5148702817210093 1101 84-85 0.5199759593509619 1101 86-87 0.4696531017306569 1101 88-89 0.5758811151184418 1101 90-91 0.7777842629896057 1101 92-93 0.7256121401356985 1101 94-95 0.598031984361878 1101 96-97 0.5996222949719403 1101 98-99 0.6081285967079779 1101 100-101 0.41257821941579564 1102 1 0.11010810311745445 1102 2 0.18049743330647772 1102 3 0.1526152616772265 1102 4 0.10856842042920789 1102 5 0.13162373962910578 1102 6 0.16915988898156087 1102 7 0.18100899964978368 1102 8 0.13532701591608998 1102 9 0.22969513954139842 1102 10-11 0.17590489208673432 1102 12-13 0.20177325715967953 1102 14-15 0.19707239450216463 1102 16-17 0.2548642630223412 1102 18-19 0.15901370549135407 1102 20-21 0.2252475023958027 1102 22-23 0.2846968348252332 1102 24-25 0.18645062138934776 1102 26-27 0.17128916938207794 1102 28-29 0.21514292214812514 1102 30-31 0.15611289631379321 1102 32-33 0.2818192036300431 1102 34-35 0.2480484148501958 1102 36-37 0.2947264705075554 1102 38-39 0.2715552894763107 1102 40-41 0.32222620498336596 1102 42-43 0.2640551031609064 1102 44-45 0.35296867066870874 1102 46-47 0.2986942660678764 1102 48-49 0.2739081328632196 1102 50-51 0.33325314721433585 1102 52-53 0.3529745619429505 1102 54-55 0.2818655237003327 1102 56-57 0.1526346632321136 1102 58-59 0.27760122122208486 1102 60-61 0.21768993098439182 1102 62-63 0.319548523361604 1102 64-65 0.13608817510350235 1102 66-67 0.17914767752191807 1102 68-69 0.14638063159935655 1102 70-71 0.017608308224815516 1102 72-73 0.17646927687351166 1102 74-75 0.2554795100234486 1102 76-77 0.18050067571163098 1102 78-79 0.26872078200590366 1102 80-81 0.36136239560476113 1102 82-83 0.3977850898043407 1102 84-85 0.1370634487123077 1102 86-87 0.07169573756077341 1102 88-89 0.298629938011544 1102 90-91 0.30687161717226985 1102 92-93 0.21824149294108608 1102 94-95 0.18182157044535074 1102 96-97 -0.10142115993426515 1102 98-99 -0.36659988238661256 1102 100-101 -0.33628335969056167 1103 1 0.17739097662504122 1103 2 0.18446553686059985 1103 3 0.1992486008010914 1103 4 0.13484111786006991 1103 5 0.09882476020765552 1103 6 0.1215786481567207 1103 7 0.1762204642698677 1103 8 0.15298064236086617 1103 9 0.18790565567438478 1103 10-11 0.16216814708781158 1103 12-13 0.21051291962354668 1103 14-15 0.21552457361504906 1103 16-17 0.16659298429753733 1103 18-19 0.19022326289282177 1103 20-21 0.2921857974635458 1103 22-23 0.25911768474050945 1103 24-25 0.266755252438692 1103 26-27 0.2058282369962896 1103 28-29 0.3091526136637057 1103 30-31 0.2700130837428105 1103 32-33 0.32222334899136484 1103 34-35 0.42546299210608396 1103 36-37 0.364267873118159 1103 38-39 0.35759986997967985 1103 40-41 0.33765576748656656 1103 42-43 0.34863173121537727 1103 44-45 0.35524629830066345 1103 46-47 0.3093690997826215 1103 48-49 0.3504834285574745 1103 50-51 0.48884154508483846 1103 52-53 0.34967380928528513 1103 54-55 0.2310978286937413 1103 56-57 0.4344361652692186 1103 58-59 0.33303129887334393 1103 60-61 0.34783294164722633 1103 62-63 0.30951601488471425 1103 64-65 0.31334813476682655 1103 66-67 0.32378442276964137 1103 68-69 0.2025731869195937 1103 70-71 0.13675971302040324 1103 72-73 0.23246674913564647 1103 74-75 0.18215989153156187 1103 76-77 0.23923487204480054 1103 78-79 0.19507524455646674 1103 80-81 0.3975058859992231 1103 82-83 0.49832691726574296 1103 84-85 0.5104186885217779 1103 86-87 0.20933437202619842 1103 88-89 0.4888902882800288 1103 90-91 0.5136749522291097 1103 92-93 0.42595745961861553 1103 94-95 0.28272546503786344 1103 96-97 0.18193859181119976 1103 98-99 -0.0765489134618953 1103 100-101 -0.38197377297541024 1104 1 0.21027430267339753 1104 2 0.1942314827657583 1104 3 0.1716081775987135 1104 4 0.11583886972200474 1104 5 0.20910725107059136 1104 6 0.14519007763936287 1104 7 0.17721155287270562 1104 8 0.16383791051006114 1104 9 0.29563076491339046 1104 10-11 0.17926702387303806 1104 12-13 0.2612028801707922 1104 14-15 0.31816652737374085 1104 16-17 0.30153530012177754 1104 18-19 0.19487893043606164 1104 20-21 0.2741235956550625 1104 22-23 0.3459398796579265 1104 24-25 0.2306387507636245 1104 26-27 0.32852275646567364 1104 28-29 0.22750170579465845 1104 30-31 0.21235716988678632 1104 32-33 0.16656819258275135 1104 34-35 0.24388170675201337 1104 36-37 0.21416265627036069 1104 38-39 0.2958517468688484 1104 40-41 0.42780731391794546 1104 42-43 0.39704401040592785 1104 44-45 0.4726636731759086 1104 46-47 0.35546902873633 1104 48-49 0.3714000111028639 1104 50-51 0.2805818089669856 1104 52-53 0.4551513499731712 1104 54-55 0.4245839234116886 1104 56-57 0.3819276190699128 1104 58-59 0.20869545352929464 1104 60-61 0.3995270895117784 1104 62-63 0.3432924027433444 1104 64-65 0.3582517381947028 1104 66-67 0.33068438418538193 1104 68-69 0.4290033316176789 1104 70-71 0.3126755196093427 1104 72-73 0.4431182108525107 1104 74-75 0.34116347801654 1104 76-77 0.3797625081966878 1104 78-79 0.5589351794330746 1104 80-81 0.6145806298268077 1104 82-83 0.6859775346850405 1104 84-85 0.5465969819586114 1104 86-87 0.6400144088734514 1104 88-89 0.7928248858291447 1104 90-91 0.9117144875664493 1104 92-93 0.8386388661357955 1104 94-95 0.9177208876066629 1104 96-97 0.674370738334563 1104 98-99 0.7517963487188446 1104 100-101 0.46345207050349657 1105 1 0.058248508846610036 1105 2 0.08097061729094435 1105 3 0.10988281895539131 1105 4 0.1007289449047235 1105 5 0.08215893817932596 1105 6 0.1233029565433128 1105 7 0.11803762194833212 1105 8 0.10634719075047627 1105 9 0.15912024282763326 1105 10-11 0.20718529324596346 1105 12-13 0.1854398307147065 1105 14-15 0.13439962335871058 1105 16-17 0.17408998029284106 1105 18-19 0.16948399942298664 1105 20-21 0.13714751596192798 1105 22-23 0.1264832273181753 1105 24-25 0.09015432355807462 1105 26-27 0.15748799285980652 1105 28-29 0.18016844772467522 1105 30-31 0.18382348819164207 1105 32-33 0.084676018090029 1105 34-35 0.2976573735719015 1105 36-37 0.10113658558194061 1105 38-39 0.20598541996247377 1105 40-41 0.30934951381203746 1105 42-43 0.26859795675928666 1105 44-45 0.20148120523675317 1105 46-47 0.27556058123117566 1105 48-49 0.27066780618225295 1105 50-51 0.33551545625601875 1105 52-53 0.3513549857307865 1105 54-55 0.35800515359121476 1105 56-57 0.34721320318074333 1105 58-59 0.2893232036657096 1105 60-61 0.21213397019516833 1105 62-63 0.4163813183328031 1105 64-65 0.3028730963219317 1105 66-67 0.2745721934035288 1105 68-69 0.3131017975892334 1105 70-71 0.2767946561236272 1105 72-73 0.39012313253830655 1105 74-75 0.3744956017602519 1105 76-77 0.4619002293835912 1105 78-79 0.4455095409713543 1105 80-81 0.4367885609715927 1105 82-83 0.467278219850364 1105 84-85 0.5479908324049525 1105 86-87 0.5819514188216566 1105 88-89 0.47257568348630485 1105 90-91 0.6394178531823869 1105 92-93 0.5462655975289508 1105 94-95 0.6378621676750456 1105 96-97 0.3960785713681858 1105 98-99 0.5419540523263784 1105 100-101 0.6077605481139905 1106 1 0.05209050855290798 1106 2 0.08160984200993227 1106 3 0.09054220618380526 1106 4 0.03232427805614435 1106 5 0.05749238431702253 1106 6 0.11245620736686135 1106 7 0.08222452353262355 1106 8 0.00927649645994677 1106 9 0.10224880575594852 1106 10-11 0.11532049486343965 1106 12-13 0.17017275114341857 1106 14-15 0.07360035872120108 1106 16-17 0.04384146516918008 1106 18-19 0.11993620772081215 1106 20-21 0.11660505401179933 1106 22-23 0.22837567293199612 1106 24-25 0.10187784718016957 1106 26-27 0.08247652187568377 1106 28-29 0.08998593331544669 1106 30-31 0.21235638616111174 1106 32-33 0.0336108298718969 1106 34-35 0.1042766522438896 1106 36-37 0.09107070173690346 1106 38-39 0.07097028537720718 1106 40-41 0.10861478648101297 1106 42-43 0.09844413632452387 1106 44-45 0.09308129446399249 1106 46-47 0.06926782545617272 1106 48-49 0.02069753165606869 1106 50-51 -0.014062340390232464 1106 52-53 0.0650021286046254 1106 54-55 0.019155839385838647 1106 56-57 0.1805409471087458 1106 58-59 0.14573798632168433 1106 60-61 0.16753305627658222 1106 62-63 0.22676493446464008 1106 64-65 0.24910657391274071 1106 66-67 0.26148206017751363 1106 68-69 0.23196294100880266 1106 70-71 0.16729858674589337 1106 72-73 0.30027153633117365 1106 74-75 0.26755452577939565 1106 76-77 0.42778974005147674 1106 78-79 0.4875112543781519 1106 80-81 0.5142155442831502 1106 82-83 0.5867714487939324 1106 84-85 0.5255214229662748 1106 86-87 0.6010495276209546 1106 88-89 0.7840024859043844 1106 90-91 0.8191611876894953 1106 92-93 0.7802101577418341 1106 94-95 0.9422247239438413 1106 96-97 0.9756062818613778 1106 98-99 0.8409144483408291 1106 100-101 0.9023068382986388 1107 1 -0.017141032920370947 1107 2 0.061540706998776784 1107 3 0.06023337674900375 1107 4 0.010502152535849518 1107 5 0.04166551529707618 1107 6 0.05315787656905968 1107 7 -0.002922694849836205 1107 8 0.018991307299145888 1107 9 0.1079655722993138 1107 10-11 0.0900909699084167 1107 12-13 0.031805233208082484 1107 14-15 0.07452210256063552 1107 16-17 0.08905674707631306 1107 18-19 0.07124438654129506 1107 20-21 0.1456694870313413 1107 22-23 0.09388939761208803 1107 24-25 0.04492472037022566 1107 26-27 0.11883128622587691 1107 28-29 0.12989253058325545 1107 30-31 0.06742528949951776 1107 32-33 0.08286711206475417 1107 34-35 0.14409975078976345 1107 36-37 0.13421721834397005 1107 38-39 0.02407887522376484 1107 40-41 0.13229125934054764 1107 42-43 0.14010106951782575 1107 44-45 0.10140437949561232 1107 46-47 0.06653782750262138 1107 48-49 0.026670344858324313 1107 50-51 0.22040484606000632 1107 52-53 0.23835621434671594 1107 54-55 0.31703970183401253 1107 56-57 0.2547295615512226 1107 58-59 0.2840263493992268 1107 60-61 0.31312816083821815 1107 62-63 0.2629144512338897 1107 64-65 0.2654037868895074 1107 66-67 0.4345431764154597 1107 68-69 0.4113740626524418 1107 70-71 0.3134088011976459 1107 72-73 0.38733391615092927 1107 74-75 0.17363825231287677 1107 76-77 0.1229388021629454 1107 78-79 0.3284224184951299 1107 80-81 0.4523455252473738 1107 82-83 0.5642319823748956 1107 84-85 0.5606264817339301 1107 86-87 0.6410369690270521 1107 88-89 0.8301899487402125 1107 90-91 0.7396935716793642 1107 92-93 0.7180878325968436 1107 94-95 0.7579021399045516 1107 96-97 0.6001051688668575 1107 98-99 0.883926184496449 1107 100-101 0.8427352060482463 1108 1 0.04548479367429792 1108 2 0.11574964147777678 1108 3 0.06709534520723537 1108 4 0.08232774059492698 1108 5 0.08378614229960135 1108 6 0.012417155640818578 1108 7 0.06186253473602221 1108 8 0.029250942685770553 1108 9 0.11702706405849028 1108 10-11 0.00862819271777937 1108 12-13 0.06517370185424198 1108 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sequence quality scores pass #Quality Count 2 3.0 3 7.0 4 28.0 5 65.0 6 162.0 7 397.0 8 785.0 9 1440.0 10 2537.0 11 3926.0 12 5868.0 13 8011.0 14 9947.0 15 13534.0 16 17290.0 17 23869.0 18 22590.0 19 16051.0 20 14944.0 21 15965.0 22 16797.0 23 19043.0 24 22236.0 25 26115.0 26 31240.0 27 38078.0 28 46147.0 29 57083.0 30 68788.0 31 85248.0 32 105624.0 33 137216.0 34 189363.0 35 273976.0 36 428393.0 37 677894.0 38 504897.0 39 30457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6829431868325 19.49402648178917 14.450421535687887 23.372608795690443 2 15.64532954917226 20.774557323798856 39.50728631618367 24.072826810845214 3 19.0122543993273 24.812775247306767 29.879691935635428 26.295278417730504 4 13.057344717823712 16.696799123735346 36.661380912437316 33.58447524600362 5 13.660565415666728 37.85046985371127 33.88563978087897 14.60332494974304 6 32.33815063988033 36.00219340510711 17.299471127367703 14.36018482764486 7 28.95390762870137 32.902859862812726 20.448907309772864 17.694325198713038 8 26.58032506016775 35.03247926793218 19.786359050402364 18.600836621497702 9 26.865954690203818 16.808252635275416 20.222708121428774 36.103084553091996 10-11 25.140242810905573 26.724048649972186 27.097537940489996 21.03817059863224 12-13 25.937272591969723 23.935704698262768 27.824454889448404 22.30256782031911 14-15 22.618962496718503 25.372195853080182 26.077398218741166 25.93144343146015 16-17 22.2204256152412 28.9090105060356 26.69134210142317 22.17922177730003 18-19 22.1811120494168 27.053553439842936 29.22485757519901 21.540476935541257 20-21 22.797575731803757 26.445689218227347 29.041492942077777 21.715242107891115 22-23 22.404522063337147 26.36695159899781 29.018139144736615 22.21038719292843 24-25 22.35474521041394 26.617361919387218 29.051798105221714 21.976094764977123 26-27 21.978862920021275 26.927057595422244 28.985381752413314 22.10869773214317 28-29 21.898746027968315 27.011615856439647 29.1985223664907 21.891115749101342 30-31 22.116886270093353 26.69860295595289 29.01911650629935 22.165394267654406 32-33 21.777878981376634 26.85919889273508 28.890619180840694 22.47230294504759 34-35 22.563426887503 26.834844805794837 28.589883556977757 22.011844749724407 36-37 22.69609681507779 26.495141703667848 28.585556104864366 22.22320537639 38-39 22.192755858526017 26.766628628650942 28.817509261585233 22.223106251237805 40-41 22.32565062238515 26.544199865599854 28.991352470464292 22.1387970415507 42-43 22.329111232662495 26.662419988991786 28.472036227647074 22.536432550698642 44-45 22.234905205984745 26.93867465848966 28.390279015926208 22.43614111959939 46-47 22.444697454222474 27.080320506995015 27.926683939156483 22.54829809962603 48-49 22.57828997060188 27.126022697168466 27.774547518392044 22.52113981383761 50-51 22.539460993394233 27.205610401189983 27.703482066692104 22.551446538723685 52-53 22.799429974813197 27.129334023545514 27.66365976265869 22.407576238982596 54-55 22.22710521965944 27.04409169503301 27.86586415565906 22.862938929648486 56-57 22.334583566673263 27.534826091593622 27.49329644247629 22.63729389925682 58-59 22.4668912355547 26.8987459522301 27.813882746919322 22.820480065295875 60-61 22.453355171478997 27.06193093034856 28.03485667087963 22.449857227292814 62-63 22.340609952011047 27.17592537817858 27.93307986825028 22.550384801560092 64-65 22.062277832390006 26.89744063256223 28.001362133611522 23.038919401436242 66-67 21.99151647254256 27.330500918380086 27.728511346349322 22.949471262728036 68-69 22.201630608861695 26.985225479309427 27.974708895782737 22.83843501604614 70-71 22.076402925363183 27.136683843081688 27.740487528523527 23.046425703031602 72-73 22.195641036016976 27.076087426192053 27.6891160330506 23.039155504740375 74-75 22.04885196862086 27.30326865770683 27.460623896288546 23.187255477383765 76-77 22.091287154482504 26.995478568455738 27.760809205765963 23.152425071295795 78-79 22.094286729861476 27.05627330775157 27.714553056154312 23.134886906232644 80-81 22.066006232826503 27.4329413882828 27.519772291785443 22.98128008710525 82-83 22.238542064612858 27.24357633399565 27.391620890709028 23.12626071068246 84-85 21.974877349697223 27.09184523805441 27.375983105705252 23.55729430654311 86-87 22.048292391619853 26.9972592233648 27.491876112845087 23.46257227217026 88-89 21.979718321494897 26.90996993203297 27.406926131249172 23.703385615222963 90-91 21.824274553686568 27.038681070478216 27.578961558274706 23.558082817560507 92-93 21.98802886405895 26.741006730420363 27.615968236092147 23.65499616942854 94-95 21.71220714303841 26.474701424615933 27.682085888476532 24.13100554386913 96-97 21.831976801208775 26.2991364238992 27.692116704515136 24.17677007037689 98-99 21.473440691163255 26.499552556634292 27.454658529295923 24.57234822290653 100-101 21.604150473704685 25.773245548832314 28.298001597734164 24.324602379728837 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 536.0 1 372.5 2 273.5 3 379.5 4 366.5 5 335.0 6 598.0 7 1311.0 8 1999.0 9 2174.0 10 1951.5 11 1885.0 12 2074.0 13 2270.0 14 2530.0 15 2720.5 16 2915.0 17 3146.0 18 3393.5 19 3744.0 20 4211.0 21 4810.0 22 5715.5 23 7028.0 24 8635.0 25 10870.0 26 14320.5 27 18782.5 28 22951.0 29 27345.0 30 32999.5 31 39146.5 32 47404.5 33 53756.0 34 58428.0 35 64143.5 36 71063.0 37 78229.0 38 82011.5 39 82966.5 40 83959.0 41 89032.0 42 94320.0 43 99016.0 44 102915.5 45 107465.0 46 108461.0 47 108404.0 48 117763.0 49 119529.5 50 112359.0 51 107864.5 52 103526.5 53 102264.5 54 100595.0 55 92308.5 56 81560.0 57 72460.0 58 64629.0 59 55256.0 60 45688.0 61 38170.0 62 32670.5 63 25443.5 64 18376.0 65 14414.5 66 10849.5 67 8982.5 68 6808.5 69 4383.5 70 3469.5 71 2930.5 72 2501.0 73 2189.0 74 1806.0 75 1540.5 76 1065.5 77 562.5 78 345.5 79 230.5 80 141.0 81 93.5 82 60.5 83 38.0 84 20.0 85 9.0 86 4.5 87 3.0 88 3.0 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2891275556290196 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014574689970624282 16-17 8.058946219051074E-4 18-19 5.144008224926218E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 3.4293388166174786E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013202954443977293 36-37 0.007836039195970939 38-39 0.002451977253881497 40-41 0.003240725181703517 42-43 3.086404934955731E-4 44-45 0.0011488285035668553 46-47 3.086404934955731E-4 48-49 3.9437396391101004E-4 50-51 5.144008224926218E-5 52-53 8.573347041543696E-5 54-55 0.0 56-57 8.573347041543696E-4 58-59 0.0019375764313888752 60-61 5.315475165757091E-4 62-63 0.0010459483390683308 64-65 1.7146694083087393E-5 66-67 3.4293388166174786E-4 68-69 0.0016460826319763897 70-71 0.0 72-73 0.0 74-75 1.7146694083087393E-5 76-77 0.25831494636171154 78-79 1.5432024674778654E-4 80-81 5.144008224926218E-5 82-83 0.0 84-85 0.0 86-87 0.10814419958203218 88-89 0.3747924392681242 90-91 0.27709057638269224 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.573347041543696E-5 100-101 3.2578718757866047E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2916014.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.574292071399654 #Duplication Level Percentage of deduplicated Percentage of total 1 69.74301409193453 31.087454800736314 2 15.377656141210597 13.708962724237473 3 5.960540891197581 7.970606717632853 4 2.9073218559459764 5.183672542099986 5 1.6390871685404669 3.653057509050312 6 0.9908272251120911 2.6499253274664483 7 0.6611361696764371 2.062877370828676 8 0.4568443400447031 1.629081043545473 9 0.33119178821781436 1.3286375549702996 >10 1.6745259016482668 13.605797060258062 >50 0.1498637128121517 4.665620065690733 >100 0.09881310152933193 8.259873932917781 >500 0.006555437235785826 1.9909059941641025 >1k 0.0024679293122632567 1.6948861800131247 >5k 1.5424558201645354E-4 0.5086411763883373 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8502 0.291562386188818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6294 0.2158425851179041 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4653 0.15956713513721127 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.144008224926218E-5 0.0 22-23 0.0 0.0 0.0 2.400537171632235E-4 0.0 24-25 0.0 0.0 0.0 4.80107434326447E-4 0.0 26-27 0.0 0.0 0.0 7.716012337389327E-4 0.0 28-29 0.0 0.0 0.0 0.002640590888795458 0.0 30-31 0.0 0.0 0.0 0.009636442074695115 0.0 32-33 0.0 0.0 0.0 0.02282224982458932 0.0 34-35 0.0 0.0 0.0 0.04320966908938023 0.0 36-37 0.0 0.0 0.0 0.07796601799579837 0.0 38-39 0.0 0.0 0.0 0.13472157541081764 0.0 40-41 0.0 0.0 0.0 0.19766708938983146 0.0 42-43 0.0 0.0 0.0 0.263699008303801 0.0 44-45 0.0 0.0 0.0 0.3437226295895699 0.0 46-47 0.0 0.0 0.0 0.441458785863168 0.0 48-49 0.0 0.0 0.0 0.5482483966126364 0.0 50-51 0.0 0.0 0.0 0.6681037882534171 0.0 52-53 0.0 0.0 0.0 0.7852671489231533 0.0 54-55 0.0 0.0 0.0 0.917828240879502 0.0 56-57 0.0 0.0 0.0 1.0797616198001792 0.0 58-59 0.0 0.0 0.0 1.259218920073772 0.0 60-61 0.0 0.0 0.0 1.462115751158945 0.0 62-63 0.0 0.0 0.0 1.6685962413074833 0.0 64-65 0.0 0.0 0.0 1.8867364834325213 0.0 66-67 0.0 0.0 0.0 2.1356036013544517 0.0 68-69 0.0 0.0 0.0 2.4100707335424314 0.0 70-71 0.0 0.0 0.0 2.726650146398474 0.0 72-73 0.0 0.0 0.0 3.0549236046191823 0.0 74-75 0.0 0.0 0.0 3.3929706784672504 0.0 76-77 0.0 0.0 0.0 3.763133510332941 0.0 78-79 0.0 0.0 0.0 4.178769374906979 0.0 80-81 0.0 0.0 0.0 4.639518191613621 0.0 82-83 0.0 0.0 0.0 5.12224907013478 0.0 84-85 0.0 0.0 0.0 5.620137626225388 0.0 86-87 0.0 0.0 0.0 6.14153429990391 0.0 88-89 0.0 0.0 0.0 6.69830460347584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8085 0.0 34.323025 1 GTATCAA 12430 0.0 33.31541 1 ATCAACG 15550 0.0 26.520931 3 TCAACGC 15620 0.0 26.371695 4 CAACGCA 15860 0.0 26.002552 5 TATCAAC 16075 0.0 25.654772 2 AACGCAG 16355 0.0 25.273592 6 GTACATG 12885 0.0 25.230934 1 TACATGG 13325 0.0 24.25377 2 ACATGGG 13440 0.0 23.163488 3 ACGCAGA 18570 0.0 22.156776 7 CGCAGAG 18815 0.0 21.843039 8 GAGTACT 9430 0.0 21.13705 12-13 TAAGGTG 1240 0.0 21.049433 5 GTATAGG 925 0.0 20.583313 1 AGTACTT 9980 0.0 20.495266 12-13 GCAGAGT 20250 0.0 20.037357 9 GTACTTT 10200 0.0 19.611204 14-15 CATGGGG 5895 0.0 19.40138 4 CATGGGA 7485 0.0 19.020802 4 >>END_MODULE