FastQCFastQC Report
Fri 27 May 2016
SRR939268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976538
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85210.2862721725709532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60140.2020468073983937No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42540.14291771178463034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126400.033.7853131
GGTATCA79700.032.4828031
GTACATG137500.027.9141241
ATCAACG152850.027.864653
TCAACGC153150.027.810074
CAACGCA155950.027.3107535
TATCAAC157400.027.0892942
TACATGG140350.026.8656562
AACGCAG160600.026.5611956
ACATGGG139950.025.8240783
ACGCAGA179600.023.5137547
CGCAGAG179900.023.4218528
CATGGGG62500.022.1587664
GCAGAGT192450.021.5989349
GAGTACT86150.021.15467312-13
AGTACTT92100.019.81373612-13
GTACACG3603.6379788E-1219.7926561
CATGGGA79000.019.6919864
GTACTTT94900.018.9547514-15
AGAGTAC169800.018.8555110-11
Produced by FastQC (version 0.11.5)