##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939268_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2976538 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01857426312044 33.0 31.0 34.0 30.0 34.0 2 32.094448651419874 34.0 31.0 34.0 30.0 34.0 3 32.15132143449873 34.0 31.0 34.0 30.0 34.0 4 35.65000816384672 37.0 35.0 37.0 33.0 37.0 5 35.69342235845805 37.0 35.0 37.0 35.0 37.0 6 35.78714365480972 37.0 35.0 37.0 35.0 37.0 7 35.70937377584294 37.0 35.0 37.0 33.0 37.0 8 35.69023812227494 37.0 35.0 37.0 33.0 37.0 9 37.34669874868052 39.0 37.0 39.0 34.0 39.0 10-11 37.2650700243034 39.0 37.0 39.0 34.0 39.0 12-13 37.17473017310715 39.0 37.0 39.0 34.0 39.0 14-15 38.44889650325311 40.0 38.0 41.0 34.0 41.0 16-17 38.394701831456544 40.0 38.0 41.0 33.5 41.0 18-19 38.33508408090204 40.0 38.0 41.0 33.5 41.0 20-21 38.2408665704923 40.0 38.0 41.0 33.5 41.0 22-23 38.17151637237623 40.0 38.0 41.0 33.0 41.0 24-25 38.189037062520285 40.0 38.0 41.0 33.0 41.0 26-27 38.11657989919833 40.0 38.0 41.0 33.0 41.0 28-29 37.99302427854104 40.0 38.0 41.0 33.0 41.0 30-31 37.82455574227508 40.0 38.0 41.0 33.0 41.0 32-33 37.644097606010746 40.0 37.0 41.0 32.5 41.0 34-35 37.35609557143232 40.0 37.0 41.0 31.5 41.0 36-37 37.11848395686532 40.0 37.0 41.0 31.0 41.0 38-39 36.81616831365835 39.5 36.0 41.0 30.5 41.0 40-41 36.69284333007003 39.0 36.0 41.0 30.0 41.0 42-43 36.53171066520905 39.0 36.0 41.0 30.0 41.0 44-45 36.51068422442448 40.0 36.0 41.0 30.0 41.0 46-47 36.34403474775057 40.0 36.0 41.0 30.0 41.0 48-49 36.161491135003146 40.0 36.0 41.0 29.0 41.0 50-51 35.01424087312172 38.0 34.0 40.0 26.5 40.5 52-53 35.11994740198176 38.5 34.5 39.5 27.5 40.5 54-55 35.62372897641488 39.0 35.0 40.5 28.0 41.0 56-57 35.531956588493074 39.0 35.0 41.0 27.5 41.0 58-59 35.33958830695258 39.0 35.0 41.0 27.0 41.0 60-61 35.05748641542624 38.0 35.0 40.0 26.5 41.0 62-63 34.71616119129001 38.0 34.0 40.0 26.0 41.0 64-65 34.35197585248366 37.0 34.0 40.0 26.0 41.0 66-67 34.01196020343097 37.0 34.0 40.0 25.5 41.0 68-69 33.61593099097004 36.0 34.0 39.0 24.5 41.0 70-71 33.21081168794082 36.0 33.0 39.0 23.5 40.5 72-73 32.78519021090945 35.0 33.0 38.5 22.5 40.0 74-75 32.34841063678677 35.0 32.5 37.5 21.5 39.5 76-77 31.916009807366812 35.0 32.0 37.0 20.0 39.0 78-79 31.497668432252503 35.0 32.0 36.5 19.0 39.0 80-81 31.053060468235245 35.0 31.0 36.0 17.5 37.5 82-83 30.62729469605293 35.0 31.0 35.5 13.0 37.0 84-85 30.22651667810053 34.0 31.0 35.0 7.5 37.0 86-87 29.91514252463768 34.0 30.5 35.0 4.5 36.0 88-89 29.522491901665624 34.0 30.0 35.0 2.0 36.0 90-91 29.14362255748121 34.0 30.0 35.0 2.0 36.0 92-93 28.75409334602817 34.0 29.0 35.0 2.0 35.0 94-95 28.31038928446403 34.0 29.0 35.0 2.0 35.0 96-97 27.781202692523998 34.0 28.5 35.0 2.0 35.0 98-99 27.100455965957767 34.0 27.0 35.0 2.0 35.0 100-101 24.656910981818477 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2771860333966316 1101 2 0.17959319519540173 1101 3 0.3672198185847293 1101 4 0.3882610911558757 1101 5 0.36278866257296016 1101 6 0.3618424335463786 1101 7 0.38755528902766656 1101 8 0.3935140612985535 1101 9 0.399621489951393 1101 10-11 0.4937614442824909 1101 12-13 0.4573268711769458 1101 14-15 0.49790277851338516 1101 16-17 0.5898626610261317 1101 18-19 0.5805095593495366 1101 20-21 0.6428527704871883 1101 22-23 0.583646260139524 1101 24-25 0.6382602563627202 1101 26-27 0.4682805429115646 1101 28-29 0.47248557728811136 1101 30-31 0.5675460820609359 1101 32-33 0.49972584382390295 1101 34-35 0.6074622551068103 1101 36-37 0.5024734259270858 1101 38-39 0.5741660555500587 1101 40-41 0.46091692978795606 1101 42-43 0.4673364586476154 1101 44-45 0.7131713561287754 1101 46-47 0.7181914846986359 1101 48-49 0.6713553476135345 1101 50-51 0.6819494735329172 1101 52-53 0.7829922695254794 1101 54-55 0.7276188660752254 1101 56-57 0.7257315288554551 1101 58-59 0.6529817034535625 1101 60-61 0.7417033257650019 1101 62-63 0.7240162966642814 1101 64-65 0.6369199504385037 1101 66-67 0.6299817756049109 1101 68-69 0.5986430379481718 1101 70-71 0.6463370532619663 1101 72-73 0.7063174888412505 1101 74-75 0.6706442986792212 1101 76-77 0.4431728296783355 1101 78-79 0.5669242921459983 1101 80-81 0.6076980519808721 1101 82-83 0.5195637070397261 1101 84-85 0.4288654256567419 1101 86-87 0.4172109263070176 1101 88-89 0.43093216029618375 1101 90-91 0.5930139717659237 1101 92-93 0.5441428297782842 1101 94-95 0.44810106461461885 1101 96-97 0.45345329826187 1101 98-99 0.34615645898812275 1101 100-101 0.016037114732355917 1102 1 0.3397453003400699 1102 2 0.3014265739283246 1102 3 0.35202700004858656 1102 4 0.2502200594860753 1102 5 0.2778098501852284 1102 6 0.3140387154225266 1102 7 0.2837329167933689 1102 8 0.3283035266825607 1102 9 0.2524203233526521 1102 10-11 0.28921957391504804 1102 12-13 0.3272181374567964 1102 14-15 0.46204717378062554 1102 16-17 0.4572439786518885 1102 18-19 0.4639239292408064 1102 20-21 0.44615820184812804 1102 22-23 0.43255370027863194 1102 24-25 0.4621552542838927 1102 26-27 0.4135015023652926 1102 28-29 0.3924237450972825 1102 30-31 0.4762458037239128 1102 32-33 0.32448974889848614 1102 34-35 0.37847321859529615 1102 36-37 0.3865012022825738 1102 38-39 0.4654912183229456 1102 40-41 0.6588255107197156 1102 42-43 0.5380865963824633 1102 44-45 0.6484805273234571 1102 46-47 0.5966243591889224 1102 48-49 0.6495246314128451 1102 50-51 0.5558191773978862 1102 52-53 0.7109666764464251 1102 54-55 0.6793666136753629 1102 56-57 0.7321630802720307 1102 58-59 0.6616799914453395 1102 60-61 0.547884291936505 1102 62-63 0.6121587093690621 1102 64-65 0.6484860928594003 1102 66-67 0.6276228744524488 1102 68-69 0.6424852153260687 1102 70-71 0.6426156524423163 1102 72-73 0.5683496768078342 1102 74-75 0.3515715562505477 1102 76-77 0.38616647851491237 1102 78-79 0.3390064128324397 1102 80-81 0.4820987262159555 1102 82-83 0.4344958022272998 1102 84-85 0.40433275126600066 1102 86-87 0.2380603969751256 1102 88-89 0.13781654700236246 1102 90-91 0.10947814944284673 1102 92-93 0.16471406582815362 1102 94-95 0.30309955925985577 1102 96-97 -0.012728958213717334 1102 98-99 -0.09961595185288985 1102 100-101 -0.33007415453771927 1103 1 0.2315593686247226 1103 2 0.2686015558091839 1103 3 0.2593353779451988 1103 4 0.2501417064665503 1103 5 0.2851946222758812 1103 6 0.23034299937386749 1103 7 0.257944979240726 1103 8 0.278422035624601 1103 9 0.3945429066517079 1103 10-11 0.40056411173016926 1103 12-13 0.3157026921178243 1103 14-15 0.48235284849806703 1103 16-17 0.49082559311585783 1103 18-19 0.5060876478751126 1103 20-21 0.5102142438453541 1103 22-23 0.5068862304935138 1103 24-25 0.383209690044211 1103 26-27 0.37690084811757885 1103 28-29 0.3951489610577923 1103 30-31 0.356681544453771 1103 32-33 0.3472268157532028 1103 34-35 0.35050119062326957 1103 36-37 0.23775534030141188 1103 38-39 0.49361015820659304 1103 40-41 0.32533791987918903 1103 42-43 0.29874015850661095 1103 44-45 0.44077401814635664 1103 46-47 0.3803455399689213 1103 48-49 0.43491571092157955 1103 50-51 0.33107823206871245 1103 52-53 0.34900266007064573 1103 54-55 0.47967659780888283 1103 56-57 0.44776610469858014 1103 58-59 0.3998388913689439 1103 60-61 0.4914529781522461 1103 62-63 0.4766251253060645 1103 64-65 0.44978283533261987 1103 66-67 0.30932352674133057 1103 68-69 0.36633489505809536 1103 70-71 0.4426930260491062 1103 72-73 0.4462609811897238 1103 74-75 0.3578814228545326 1103 76-77 0.4850039155876438 1103 78-79 0.40778567278817235 1103 80-81 0.31651186209968074 1103 82-83 0.2693472253140179 1103 84-85 0.2538337404728743 1103 86-87 0.3230415922504406 1103 88-89 0.33222263111433037 1103 90-91 0.3167659596718302 1103 92-93 0.4609105164363676 1103 94-95 0.34067962625168846 1103 96-97 0.13293665068718497 1103 98-99 -0.019110672818197116 1103 100-101 -0.44492988704050873 1104 1 0.20108799971661284 1104 2 0.23615323629398688 1104 3 0.23077660160237912 1104 4 0.27199579882829283 1104 5 0.26241801811329424 1104 6 0.1753235746928823 1104 7 0.20489133674308846 1104 8 0.2105180192918965 1104 9 0.2111442659605629 1104 10-11 0.21105369075318947 1104 12-13 0.21132169641934695 1104 14-15 0.1819722984196943 1104 16-17 0.3353212773577141 1104 18-19 0.11201079348399645 1104 20-21 0.20065283063767936 1104 22-23 0.3487370225860502 1104 24-25 0.2973885903230311 1104 26-27 0.21421921464625626 1104 28-29 0.2102457242810587 1104 30-31 0.3345280685331744 1104 32-33 0.4150149596896142 1104 34-35 0.3749876804602863 1104 36-37 0.49716452789351706 1104 38-39 0.5544662011335433 1104 40-41 0.5171246750221101 1104 42-43 0.5639486897304522 1104 44-45 0.6286019858897163 1104 46-47 0.6210768651079803 1104 48-49 0.5508045806380082 1104 50-51 0.6453058825171496 1104 52-53 0.642902871879798 1104 54-55 0.7097315380773566 1104 56-57 0.6800818829450677 1104 58-59 0.662078366944435 1104 60-61 0.7487038678568041 1104 62-63 0.6949590840711224 1104 64-65 0.6936298714842408 1104 66-67 0.6765886254729949 1104 68-69 0.8238820411628609 1104 70-71 0.7143628394191595 1104 72-73 0.6909276400384954 1104 74-75 0.6361110797102896 1104 76-77 0.7079775865871021 1104 78-79 0.7345071596627548 1104 80-81 0.5904973783293563 1104 82-83 0.4845661012903477 1104 84-85 0.3521272472071537 1104 86-87 0.3254487365794212 1104 88-89 0.3471060216020696 1104 90-91 0.38350371335559785 1104 92-93 0.4855928027020546 1104 94-95 0.4500709862474004 1104 96-97 0.2500966964330509 1104 98-99 0.2303054280281991 1104 100-101 0.22584103574491365 1105 1 0.2911056344048788 1105 2 0.2930597357044391 1105 3 0.26693421610415413 1105 4 0.2704097362260214 1105 5 0.2689210356952856 1105 6 0.21999320152699653 1105 7 0.3153741136560342 1105 8 0.25354526408741407 1105 9 0.426739091062565 1105 10-11 0.3533572173824311 1105 12-13 0.37416860906159144 1105 14-15 0.4654095835761254 1105 16-17 0.46375118131564363 1105 18-19 0.37514002741495744 1105 20-21 0.4749292901458233 1105 22-23 0.4963048073857266 1105 24-25 0.3725990406358122 1105 26-27 0.39293365914686973 1105 28-29 0.3628575180269493 1105 30-31 0.4663860829431741 1105 32-33 0.4601845556150863 1105 34-35 0.48962220553524105 1105 36-37 0.4126875188954173 1105 38-39 0.5803176269740575 1105 40-41 0.4502427295449465 1105 42-43 0.32520778314039234 1105 44-45 0.43089327511000164 1105 46-47 0.48499904988374 1105 48-49 0.5374016945887163 1105 50-51 0.5444543996867353 1105 52-53 0.4448409577779344 1105 54-55 0.5211636729554314 1105 56-57 0.47189856157066856 1105 58-59 0.4833391150730506 1105 60-61 0.48081262444299 1105 62-63 0.4566714148496587 1105 64-65 0.3688247534956943 1105 66-67 0.43777188878472373 1105 68-69 0.3109275651019061 1105 70-71 0.17774937271781965 1105 72-73 0.429504750934548 1105 74-75 0.37921955858801937 1105 76-77 0.2118180357694719 1105 78-79 0.2528118480815138 1105 80-81 0.37960495797341665 1105 82-83 0.19381908967339356 1105 84-85 0.17124752152035327 1105 86-87 0.12495590128333234 1105 88-89 0.30331269837852304 1105 90-91 0.3115415535803052 1105 92-93 0.278572611781847 1105 94-95 0.2674423409215976 1105 96-97 0.3098059594114275 1105 98-99 0.20411031953215897 1105 100-101 -0.038725416563718085 1106 1 0.09458746972163823 1106 2 0.19456305474516 1106 3 0.15186219999477402 1106 4 0.24604053366392975 1106 5 0.153139703872462 1106 6 0.2143090579792144 1106 7 0.19819971361310706 1106 8 0.2094262063624015 1106 9 0.24635523293684258 1106 10-11 0.2354183888707979 1106 12-13 0.29369050875713754 1106 14-15 0.2863941795926692 1106 16-17 0.24918564573228963 1106 18-19 0.3689434175011357 1106 20-21 0.2825631850344692 1106 22-23 0.31324889812144363 1106 24-25 0.36740736847052347 1106 26-27 0.4697883014378803 1106 28-29 0.323061046711274 1106 30-31 0.22707207286316589 1106 32-33 0.31245459368908257 1106 34-35 0.39039039255464303 1106 36-37 0.35747314356527937 1106 38-39 0.4960892017511256 1106 40-41 0.5020365706344379 1106 42-43 0.3173009693815132 1106 44-45 0.38657616580755416 1106 46-47 0.30908963880180806 1106 48-49 0.27176681969277183 1106 50-51 0.4505434669612498 1106 52-53 0.2926601504817441 1106 54-55 0.32308539335418374 1106 56-57 0.4848055132696203 1106 58-59 0.5288827520998183 1106 60-61 0.5377512507268776 1106 62-63 0.510572448792793 1106 64-65 0.5438441979844342 1106 66-67 0.38709086976949436 1106 68-69 0.30349398704515806 1106 70-71 0.2487679475513218 1106 72-73 0.2525572458655674 1106 74-75 0.2587224478631427 1106 76-77 0.29787486303128574 1106 78-79 0.28033943546402185 1106 80-81 0.2162596099328873 1106 82-83 0.30212577779290584 1106 84-85 0.3928834984183034 1106 86-87 0.42202586423693234 1106 88-89 0.43614601791784935 1106 90-91 0.47001843950588906 1106 92-93 0.5159921604469808 1106 94-95 0.4691353465498054 1106 96-97 0.4234167598409897 1106 98-99 0.47153955437476114 1106 100-101 0.4505012736514722 1107 1 0.10572057024566561 1107 2 0.09493577049286728 1107 3 0.1144623136387466 1107 4 0.07538744569979627 1107 5 0.18129191382045917 1107 6 0.013408404274301233 1107 7 0.01840913636461039 1107 8 0.030440307193458693 1107 9 0.10753746155423727 1107 10-11 0.19976895340576561 1107 12-13 0.2549222255565411 1107 14-15 0.18222863820300716 1107 16-17 0.25840007395594 1107 18-19 0.1407807529796372 1107 20-21 0.26367947662080127 1107 22-23 0.3406715084712033 1107 24-25 0.13328559705527 1107 26-27 0.2042594188481459 1107 28-29 0.2786119451204243 1107 30-31 0.300419488062893 1107 32-33 0.24917723733504005 1107 34-35 0.25476120607410024 1107 36-37 0.3548273322723716 1107 38-39 0.32647418931966143 1107 40-41 0.3063504805465058 1107 42-43 0.3693170336558964 1107 44-45 0.4820687922724787 1107 46-47 0.2955914760940743 1107 48-49 0.25493759701131324 1107 50-51 0.21535799370514752 1107 52-53 0.326870131610697 1107 54-55 0.3170913578624095 1107 56-57 0.32804494508815907 1107 58-59 0.30009047259229504 1107 60-61 0.23203707612566404 1107 62-63 0.23697457994138915 1107 64-65 0.32004544768405907 1107 66-67 0.11696803633281405 1107 68-69 0.2785589290312771 1107 70-71 0.21262315014799782 1107 72-73 0.20320517736038113 1107 74-75 0.1928884021544519 1107 76-77 0.24030743223252315 1107 78-79 0.26152102312363823 1107 80-81 0.20232874071011864 1107 82-83 0.3068320762953469 1107 84-85 0.02210964872746146 1107 86-87 0.075805138543096 1107 88-89 0.07977631395152329 1107 90-91 0.18618103008918396 1107 92-93 0.1463486890100505 1107 94-95 -0.024450454530985155 1107 96-97 0.012979026488551426 1107 98-99 0.3145938406178601 1107 100-101 0.2474357070315918 1108 1 0.13264298810634045 1108 2 0.192423075688275 1108 3 0.21127927607182784 1108 4 0.25264101497336355 1108 5 0.2514321811292106 1108 6 0.13033054485578077 1108 7 0.15001779309076113 1108 8 0.18950900178040087 1108 9 0.22277829245906133 1108 10-11 0.21512894475558397 1108 12-13 0.20935887253870789 1108 14-15 0.26183433558281166 1108 16-17 0.2719681919993846 1108 18-19 0.21101986626165115 1108 20-21 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-1.2650553297439089 2316 46-47 -1.3213755810006802 2316 48-49 -1.1527318309859638 2316 50-51 -1.333904350068991 2316 52-53 -1.2815984306256993 2316 54-55 -1.1078401088464105 2316 56-57 -1.190599890481316 2316 58-59 -0.763503888940555 2316 60-61 -0.8304211999339941 2316 62-63 -1.0015938244693032 2316 64-65 -1.1412691069334784 2316 66-67 -1.0869623623747842 2316 68-69 -1.0742860289205751 2316 70-71 -1.0341337024523156 2316 72-73 -1.215454074391772 2316 74-75 -1.0629954551345548 2316 76-77 -0.902641479279854 2316 78-79 -0.892989140285156 2316 80-81 -0.731022933367921 2316 82-83 -0.6361400722603641 2316 84-85 -0.6632723984513547 2316 86-87 -0.5429690194949401 2316 88-89 -0.634943088170921 2316 90-91 -0.7651436177370137 2316 92-93 -0.7005864803913795 2316 94-95 -0.7690287520352967 2316 96-97 -0.7285960406811753 2316 98-99 -0.6997753695756295 2316 100-101 -0.3929540734465249 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7451.0 3 1884.0 4 1987.0 5 2124.0 6 2505.0 7 3027.0 8 3939.0 9 4700.0 10 5965.0 11 7681.0 12 10442.0 13 15268.0 14 13890.0 15 15141.0 16 18511.0 17 24272.0 18 20050.0 19 15047.0 20 14976.0 21 15830.0 22 16794.0 23 18995.0 24 21986.0 25 24877.0 26 29363.0 27 34662.0 28 41454.0 29 50594.0 30 61292.0 31 75159.0 32 94794.0 33 123281.0 34 170064.0 35 247018.0 36 388673.0 37 625395.0 38 636138.0 39 111281.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63978794407842 18.853402109440935 13.95367720264076 23.553132743839882 2 15.23771150128411 20.246476267864182 40.35186199158636 24.163950239265347 3 19.02857417718351 24.705669055668668 29.558694472690068 26.707062294457756 4 12.690865692848268 16.072410055898242 37.07353959195426 34.163184659299226 5 13.74894161410199 37.66366926622278 33.901147345818785 14.686241773856448 6 32.3608230441608 35.60314776405888 17.603712506008787 14.432316685771529 7 29.069484810230843 32.72007221296774 20.59178997725695 17.61865299954447 8 26.625009161468444 34.7463063312639 20.040317185166558 18.588367322101092 9 27.076586367051775 16.818877889785963 20.38517592120989 35.719359821952374 10-11 25.129739478890546 26.528122126729002 27.31007205974555 21.0320663346349 12-13 26.021293323030203 23.856627356425054 27.854206109563606 22.267873210981136 14-15 22.757261258741444 25.274992923470613 26.141069352677416 25.826676465110527 16-17 22.388853235204834 28.708839465300073 26.726776648850283 22.17553065064481 18-19 22.30136446546711 27.033330234733242 29.1490913565363 21.51621394326334 20-21 22.90460588801904 26.401711973560566 28.971571892298975 21.722110246121414 22-23 22.50660119438496 26.389075220832776 28.847799472980412 22.256524111801856 24-25 22.468145061969697 26.531622073536422 28.961046057570496 22.03918680692339 26-27 22.08085589175455 26.829574212952036 28.878730285550574 22.210839609742845 28-29 21.98093976363424 26.95618768372819 29.092154738117195 21.97071781452038 30-31 22.132664452005972 26.672461097736473 28.909896036474652 22.2849784137829 32-33 21.813255745265213 26.858357340055722 28.8251126594282 22.50327425525087 34-35 22.67033650554601 26.860449022051696 28.462793040148092 22.006421432254193 36-37 22.72674704027492 26.465633741270533 28.45151216393734 22.356107054517203 38-39 22.22684590136968 26.748180500793328 28.691035871547786 22.333937726289204 40-41 22.41285900062806 26.535355460523647 28.852891578183016 22.19889396066528 42-43 22.33819347339778 26.6566299582935 28.365072794227704 22.640103774081023 44-45 22.260289848524977 26.913169343977962 28.344020607764524 22.48252019973254 46-47 22.46301400947069 27.07571581043307 27.805767348143632 22.655502831952607 48-49 22.554742248834764 27.109213051985222 27.757985097463333 22.57805960171668 50-51 22.539989962153236 27.2176366687229 27.716814708328396 22.525558660795465 52-53 22.81007081494849 27.147680289490918 27.68808176526878 22.35416713029182 54-55 22.246121418857097 27.006835769590467 27.89616683107666 22.85087598047578 56-57 22.344377039643145 27.495299677971484 27.590704923591534 22.569618358793832 58-59 22.49494307462411 26.830654168315238 27.895641641429787 22.77876111563087 60-61 22.447066258639254 26.979077814924263 28.134386281223595 22.43946964521289 62-63 22.370328120214726 27.07433796899569 28.08131312135329 22.474020789436292 64-65 22.09103299629148 26.90736733065687 28.052597007154695 22.949002665896952 66-67 22.026950538523497 27.29245740008454 27.797655649467984 22.88293641192398 68-69 22.303562367870043 26.931755387344293 28.048286739105542 22.716395505680122 70-71 22.107970625546884 27.105452155218714 27.89824334375965 22.888333875474753 72-73 22.26897160316525 27.070414964777513 27.74923733222911 22.91137609982813 74-75 22.15421174587743 27.361253654347728 27.575485811798416 22.909048787976428 76-77 22.321656455762696 27.055995943285566 27.758587729315458 22.863759871636276 78-79 22.268231118973524 27.170077557426474 27.733336344661318 22.82835497893868 80-81 22.270033892146156 27.5076336965024 27.568544838876207 22.65378757247524 82-83 22.46279056164646 27.326933148676098 27.460638830645035 22.749637459032407 84-85 22.291750673968952 27.188157027011357 27.410256600445308 23.109835698574383 86-87 22.405696635259183 27.11321191083996 27.542586876283643 22.93850457761721 88-89 22.3629602517673 27.070261547272374 27.455728382103107 23.111049818857225 90-91 22.281913101486598 27.213228868861023 27.592947400265288 22.91191062938709 92-93 22.528808624131578 26.855898620487658 27.671185339205646 22.944107416175118 94-95 22.309252560366335 26.681667090745652 27.740583043686545 23.268497305201468 96-97 22.521312954917512 26.52170349850157 27.687246762164353 23.26973678441657 98-99 22.27652264259523 26.706066694982173 27.43296804760107 23.58444261482153 100-101 22.47608987583627 26.189302573193622 28.005349286565117 23.32925826440499 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2347.0 1 1337.5 2 428.5 3 516.5 4 448.5 5 436.0 6 753.0 7 1446.5 8 2289.0 9 2657.5 10 2479.5 11 2424.0 12 2639.5 13 2931.0 14 3204.5 15 3448.0 16 3645.5 17 3857.5 18 4036.0 19 4254.5 20 4639.0 21 5163.0 22 6004.0 23 7258.5 24 8914.5 25 11272.0 26 14828.5 27 19273.0 28 23420.5 29 27879.0 30 33533.0 31 39840.0 32 48202.0 33 54544.5 34 59214.5 35 64969.0 36 72430.5 37 80257.5 38 83768.5 39 84357.0 40 85558.5 41 90796.0 42 96223.0 43 100494.0 44 104510.0 45 109353.0 46 110227.0 47 110358.5 48 119438.0 49 120887.0 50 113557.0 51 108905.5 52 104512.0 53 103237.5 54 101644.5 55 93586.0 56 82483.0 57 73046.0 58 65676.5 59 56380.5 60 46803.5 61 39363.0 62 33715.5 63 26510.0 64 19152.5 65 14884.0 66 11355.0 67 9385.5 68 7248.0 69 4970.0 70 3831.5 71 3044.0 72 2637.5 73 2267.5 74 1761.5 75 1513.0 76 1123.5 77 634.0 78 386.5 79 262.0 80 171.5 81 125.0 82 88.0 83 63.0 84 48.0 85 28.0 86 18.0 87 16.5 88 16.5 89 10.5 90 9.5 91 9.5 92 7.5 93 6.5 94 4.5 95 2.5 96 4.0 97 5.5 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28398763933133053 2 0.10985917196420808 3 0.12124824208526819 4 0.1221553361657066 5 0.12937849273216065 6 0.058054021148058584 7 0.06729294233770912 8 0.07135806766115535 9 0.06897274619037284 10-11 0.06460525617344713 12-13 0.06845200699604709 14-15 0.06437008363407422 16-17 0.06500840909808643 18-19 0.06976225400112479 20-21 0.07367619697783129 22-23 0.08247836916578925 24-25 0.09089418646763456 26-27 0.09084379235205464 28-29 0.08551881413911061 30-31 0.08123531431481809 32-33 0.07382737932457102 34-35 0.23876731961762288 36-37 0.3602171381652107 38-39 0.33344106475375085 40-41 0.31797007127071786 42-43 0.33911880177575426 44-45 0.3518013208633654 46-47 0.3578486147329549 48-49 0.3673899006160849 50-51 0.3615777792858683 52-53 0.36216571063430064 54-55 0.34859289550477773 56-57 0.3265538689578295 58-59 0.3131154381365197 60-61 0.27246418490205737 62-63 0.33863165865848177 64-65 0.3672723143463984 66-67 0.3718581788641704 68-69 0.38358320975576327 70-71 0.38449030383620164 72-73 0.3682466005809434 74-75 0.371690198478904 76-77 0.35581605207123174 78-79 0.334684119604722 80-81 0.3333234784840644 82-83 0.3467619093053742 84-85 0.35363230706276894 86-87 0.34825693473424496 88-89 0.36228329690398714 90-91 0.38457429402883486 92-93 0.3804251785127554 94-95 0.3792493158158908 96-97 0.3843391214894619 98-99 0.3743442885661127 100-101 0.3539346717562483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2976538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.65654037660935 #Duplication Level Percentage of deduplicated Percentage of total 1 70.78960626191524 32.320085165414106 2 14.977858077757178 13.676743641644903 3 5.717488210192461 7.8312219396432 4 2.7978115780804407 5.1095358912309905 5 1.5327887446171715 3.4990915603713124 6 0.9557560836553928 2.618190973416148 7 0.6084851285606689 1.944692808848756 8 0.43492932781886123 1.588589473322672 9 0.3154695598138032 1.2962923825707078 >10 1.624193958061623 13.364505298976356 >50 0.14387049865184953 4.6119717349537614 >100 0.09304008021888012 8.02241535920944 >500 0.006416243111238239 2.06488102355267 >1k 0.002138747703684782 1.5623651342186367 >5k 1.4749984163343327E-4 0.489417612626463 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8521 0.2862721725709532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6014 0.2020468073983937 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4254 0.14291771178463034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.6798038526637322E-5 0.0 18-19 0.0 0.0 0.0 3.3596077053274644E-5 0.0 20-21 0.0 0.0 0.0 5.0394115579911966E-5 0.0 22-23 0.0 0.0 0.0 2.183745008462852E-4 0.0 24-25 0.0 0.0 0.0 4.0315292463929573E-4 0.0 26-27 0.0 0.0 0.0 9.238921189650527E-4 0.0 28-29 0.0 0.0 0.0 0.002855666549528345 0.0 30-31 0.0 0.0 0.0 0.010112419193035668 0.0 32-33 0.0 0.0 0.0 0.02425636763246429 0.0 34-35 0.0 0.0 0.0 0.044951551097281474 0.0 36-37 0.0 0.0 0.0 0.07933713596130806 0.0 38-39 0.0 0.0 0.0 0.1317470161644165 0.0 40-41 0.0 0.0 0.0 0.1916152254733519 0.0 42-43 0.0 0.0 0.0 0.2532304307890576 0.0 44-45 0.0 0.0 0.0 0.33226520205688626 0.0 46-47 0.0 0.0 0.0 0.4259142668428893 0.0 48-49 0.0 0.0 0.0 0.5317083134836511 0.0 50-51 0.0 0.0 0.0 0.648202710665881 0.0 52-53 0.0 0.0 0.0 0.7645627235398977 0.0 54-55 0.0 0.0 0.0 0.8939748123491116 0.0 56-57 0.0 0.0 0.0 1.0508516941493775 0.0 58-59 0.0 0.0 0.0 1.2237875007811088 0.0 60-61 0.0 0.0 0.0 1.4199381966566529 0.0 62-63 0.0 0.0 0.0 1.6197676629695303 0.0 64-65 0.0 0.0 0.0 1.831590928790427 0.0 66-67 0.0 0.0 0.0 2.087038700665001 0.0 68-69 0.0 0.0 0.0 2.370572792956112 0.0 70-71 0.0 0.0 0.0 2.6820420233170212 0.0 72-73 0.0 0.0 0.0 3.0056898316097422 0.0 74-75 0.0 0.0 0.0 3.342725676608194 0.0 76-77 0.0 0.0 0.0 3.7172379455595728 0.0 78-79 0.0 0.0 0.0 4.140212555660301 0.0 80-81 0.0 0.0 0.0 4.594851468383739 0.0 82-83 0.0 0.0 0.0 5.0732764036608975 0.0 84-85 0.0 0.0 0.0 5.559663609199681 0.0 86-87 0.0 0.0 0.0 6.083712017115186 0.0 88-89 0.0 0.0 0.0 6.639071968844342 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12640 0.0 33.785313 1 GGTATCA 7970 0.0 32.482803 1 GTACATG 13750 0.0 27.914124 1 ATCAACG 15285 0.0 27.86465 3 TCAACGC 15315 0.0 27.81007 4 CAACGCA 15595 0.0 27.310753 5 TATCAAC 15740 0.0 27.089294 2 TACATGG 14035 0.0 26.865656 2 AACGCAG 16060 0.0 26.561195 6 ACATGGG 13995 0.0 25.824078 3 ACGCAGA 17960 0.0 23.513754 7 CGCAGAG 17990 0.0 23.421852 8 CATGGGG 6250 0.0 22.158766 4 GCAGAGT 19245 0.0 21.598934 9 GAGTACT 8615 0.0 21.154673 12-13 AGTACTT 9210 0.0 19.813736 12-13 GTACACG 360 3.6379788E-12 19.792656 1 CATGGGA 7900 0.0 19.691986 4 GTACTTT 9490 0.0 18.95475 14-15 AGAGTAC 16980 0.0 18.85551 10-11 >>END_MODULE