FastQCFastQC Report
Fri 27 May 2016
SRR939268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2976538
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87870.2952087290671243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66070.2219692810909856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46860.15743121707164498No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.034.8531041
GTATCAA126950.034.7791441
ATCAACG159100.027.6192673
TCAACGC159950.027.4724944
GTACATG135850.027.457441
CAACGCA161650.027.183585
TATCAAC164500.026.7991582
AACGCAG166600.026.3759026
TACATGG141050.025.8716752
ACATGGG138400.025.3384283
ACGCAGA190000.023.1025287
CGCAGAG191850.022.780818
CATGGGG59100.021.3582424
GCAGAGT205500.021.0836059
GAGTACT97450.020.91580412-13
GCACCGT7550.020.7414136
AGTACTT101550.020.445212-13
CATGGGA77600.020.0578064
AGAGTAC181900.019.64499310-11
GTACTTT102600.019.58841514-15
Produced by FastQC (version 0.11.5)