##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939268_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2976538 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27947837386924 33.0 31.0 34.0 31.0 34.0 2 32.48288783815291 34.0 31.0 34.0 31.0 34.0 3 32.57429873228563 34.0 31.0 34.0 31.0 34.0 4 36.11295538642544 37.0 35.0 37.0 35.0 37.0 5 36.04983171725004 37.0 35.0 37.0 35.0 37.0 6 36.08227108137037 37.0 35.0 37.0 35.0 37.0 7 36.025277352414115 37.0 35.0 37.0 35.0 37.0 8 36.00274379161294 37.0 35.0 37.0 35.0 37.0 9 37.71140230697542 39.0 37.0 39.0 35.0 39.0 10-11 37.63168267967686 39.0 37.0 39.0 35.0 39.0 12-13 37.55061282604153 39.0 37.0 39.0 35.0 39.0 14-15 38.85477289387873 40.0 38.0 41.0 35.0 41.0 16-17 38.80042468801003 40.0 38.0 41.0 35.0 41.0 18-19 38.904394299686416 40.0 38.0 41.0 35.0 41.0 20-21 38.90368811014675 40.0 38.0 41.0 35.0 41.0 22-23 38.84943800482306 40.0 38.0 41.0 35.0 41.0 24-25 38.780697407525125 40.0 38.0 41.0 34.0 41.0 26-27 38.61829817055922 40.0 38.0 41.0 34.0 41.0 28-29 38.469987616485994 40.0 38.0 41.0 34.0 41.0 30-31 38.279843395246424 40.0 38.0 41.0 34.0 41.0 32-33 38.08925066637819 40.0 37.5 41.0 33.0 41.0 34-35 38.043303159576666 40.0 38.0 41.0 33.0 41.0 36-37 37.95860039414917 40.0 37.5 41.0 33.0 41.0 38-39 37.856472855377625 40.0 37.0 41.0 33.0 41.0 40-41 37.6791075739668 40.0 37.0 41.0 33.0 41.0 42-43 37.50480138335207 40.0 37.0 41.0 32.5 41.0 44-45 37.28635834650859 40.0 37.0 41.0 32.0 41.0 46-47 37.00819929058524 40.0 36.0 41.0 31.0 41.0 48-49 36.707948126313184 40.0 36.0 41.0 30.5 41.0 50-51 36.46928797818136 39.0 36.0 41.0 30.0 41.0 52-53 36.235835557953564 39.0 35.0 41.0 30.0 41.0 54-55 35.957416972334975 39.0 35.0 40.5 29.0 41.0 56-57 35.72416461674604 38.5 35.0 40.0 28.5 41.0 58-59 35.43442734478781 38.0 35.0 40.0 28.0 41.0 60-61 35.10895459758955 38.0 34.0 40.0 28.0 41.0 62-63 34.73401465057728 37.5 34.0 40.0 27.0 41.0 64-65 34.32272022060528 37.0 34.0 39.5 26.0 41.0 66-67 33.95240544552094 36.0 33.0 39.0 26.0 40.5 68-69 33.57217327647085 36.0 33.0 39.0 26.0 40.0 70-71 33.18236521757828 35.0 33.0 38.5 25.0 40.0 72-73 32.69179882803445 35.0 32.0 37.5 24.0 40.0 74-75 32.19812009791241 35.0 32.0 37.0 22.5 39.0 76-77 30.190961109853127 33.5 29.5 35.0 20.0 37.5 78-79 31.18784591360836 34.0 31.0 36.0 20.0 38.0 80-81 31.236587270177637 35.0 31.0 36.0 20.5 37.0 82-83 30.934197547620762 34.0 31.0 35.0 20.0 37.0 84-85 30.564108874134988 34.0 31.0 35.0 18.5 36.5 86-87 30.039323704249703 34.0 30.5 35.0 14.0 36.0 88-89 29.54591122303831 34.0 30.0 35.0 7.0 36.0 90-91 29.132562896895656 34.0 29.0 35.0 2.0 35.0 92-93 28.78900605334116 34.0 29.0 35.0 2.0 35.0 94-95 28.400812285950995 34.0 29.0 35.0 2.0 35.0 96-97 27.861187560850894 33.5 29.0 35.0 2.0 35.0 98-99 27.11731985279543 33.0 27.0 35.0 2.0 35.0 100-101 23.723254666999043 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2603787235835284 1101 2 0.2529289800026149 1101 3 0.24392304604744908 1101 4 0.20793758846328814 1101 5 0.1923807756742022 1101 6 0.2402460675616922 1101 7 0.20126246156017658 1101 8 0.27660014697801927 1101 9 0.2717288861458371 1101 10-11 0.3222520224544354 1101 12-13 0.35767741196047353 1101 14-15 0.34423970398572834 1101 16-17 0.4068252860744508 1101 18-19 0.39918498521807066 1101 20-21 0.39358080413924057 1101 22-23 0.380724424794181 1101 24-25 0.451849462643203 1101 26-27 0.49037136383839197 1101 28-29 0.3736141182992512 1101 30-31 0.4137504662526581 1101 32-33 0.3906383979868693 1101 34-35 0.4458389480999756 1101 36-37 0.4915647016911464 1101 38-39 0.5413283984833228 1101 40-41 0.5510675121849431 1101 42-43 0.6566224711291966 1101 44-45 0.7281049203857748 1101 46-47 0.6881848582523062 1101 48-49 0.7386992471063678 1101 50-51 0.6963451372033518 1101 52-53 0.6315139187611152 1101 54-55 0.630265226501109 1101 56-57 0.5882101389212835 1101 58-59 0.5245926797256573 1101 60-61 0.5633691024733309 1101 62-63 0.7326864245941138 1101 64-65 0.7750825806056412 1101 66-67 0.7313741062048464 1101 68-69 0.8355664939959198 1101 70-71 0.749752002247206 1101 72-73 0.6203014086295937 1101 74-75 0.6582228991437304 1101 76-77 0.6891124833780147 1101 78-79 0.8516365159039445 1101 80-81 0.8984004513049797 1101 82-83 0.8775997200679129 1101 84-85 0.7934221153087897 1101 86-87 0.8862951521446938 1101 88-89 1.0771030896514624 1101 90-91 1.1637374019718187 1101 92-93 0.9817271820715163 1101 94-95 1.067180642575142 1101 96-97 0.7933946572014321 1101 98-99 0.5492987313970481 1101 100-101 0.4740724687309914 1102 1 0.13168625220063035 1102 2 0.12448842513089886 1102 3 0.11412562378826863 1102 4 0.0936105025228926 1102 5 0.10956168654696086 1102 6 0.11596213512446951 1102 7 0.1238524090673323 1102 8 0.17037842358234911 1102 9 0.23811953697111932 1102 10-11 0.2526073476213 1102 12-13 0.17547316296865745 1102 14-15 0.21701008925414556 1102 16-17 0.2818665041215027 1102 18-19 0.2661510502207278 1102 20-21 0.2647633985516151 1102 22-23 0.17589069993207573 1102 24-25 0.27087663480867974 1102 26-27 0.28279779090446056 1102 28-29 0.34741275303464647 1102 30-31 0.31995047977013513 1102 32-33 0.29018105687348594 1102 34-35 0.3701948713125418 1102 36-37 0.353377742774029 1102 38-39 0.38837513821260217 1102 40-41 0.38268743775692826 1102 42-43 0.3106253835298389 1102 44-45 0.37124418925442626 1102 46-47 0.281562935781146 1102 48-49 0.3829910852162115 1102 50-51 0.3902824489059995 1102 52-53 0.37151098997566123 1102 54-55 0.26958734579484656 1102 56-57 0.2691307401620264 1102 58-59 0.3065067127041914 1102 60-61 0.22631863519892192 1102 62-63 0.17981416674629713 1102 64-65 0.17352489684483885 1102 66-67 0.31769698534431967 1102 68-69 0.4130666629643187 1102 70-71 0.22005606342809614 1102 72-73 0.4280061624784892 1102 74-75 0.2927755015641722 1102 76-77 0.4385589838890134 1102 78-79 0.4908326769420839 1102 80-81 0.6412474560586219 1102 82-83 0.6144483469181168 1102 84-85 0.5522991001753681 1102 86-87 0.6352076101330049 1102 88-89 0.7332606050583088 1102 90-91 0.7770051072991357 1102 92-93 0.6890257973499452 1102 94-95 0.6306953489695175 1102 96-97 0.5107417715285187 1102 98-99 0.4736095963718441 1102 100-101 0.3563091801350282 1103 1 0.20798740203118626 1103 2 0.20888931835532532 1103 3 0.21600903449520814 1103 4 0.1919043655226389 1103 5 0.13556999196702435 1103 6 0.13432122701296834 1103 7 0.20287632632653896 1103 8 0.09287749314024296 1103 9 0.23706177120747185 1103 10-11 0.2019643631547936 1103 12-13 0.24148803045411427 1103 14-15 0.36109639510515734 1103 16-17 0.25859076025752614 1103 18-19 0.2698973039669852 1103 20-21 0.273617289758441 1103 22-23 0.29822914579992954 1103 24-25 0.20129670493463436 1103 26-27 0.25227439273400876 1103 28-29 0.23684929688295142 1103 30-31 0.33845403503838867 1103 32-33 0.3101390400667654 1103 34-35 0.34262929962932986 1103 36-37 0.40760048082029243 1103 38-39 0.34009498699128216 1103 40-41 0.419787592504143 1103 42-43 0.37919651827743905 1103 44-45 0.4256799495725403 1103 46-47 0.5856216221927752 1103 48-49 0.581647820637663 1103 50-51 0.6181207870384569 1103 52-53 0.5183004750004443 1103 54-55 0.5005818414952188 1103 56-57 0.571896601751412 1103 58-59 0.4926538988513798 1103 60-61 0.3992706831509736 1103 62-63 0.4493044803542574 1103 64-65 0.5502927368479718 1103 66-67 0.6812892754071882 1103 68-69 0.5504293003269538 1103 70-71 0.5137035923697439 1103 72-73 0.5996653856082972 1103 74-75 0.6404792683855831 1103 76-77 0.6592206751389433 1103 78-79 0.8182944308744275 1103 80-81 0.8708046635570099 1103 82-83 0.7954976897321657 1103 84-85 0.7013534771709224 1103 86-87 0.7237367280738916 1103 88-89 0.8482534553452759 1103 90-91 0.8287303428479014 1103 92-93 0.7768154586690201 1103 94-95 0.7793677549948619 1103 96-97 0.4330253702827065 1103 98-99 0.2773573192372183 1103 100-101 0.2781569402180821 1104 1 0.08475487489052114 1104 2 0.1068368692037609 1104 3 0.09149967731059405 1104 4 0.04737546918069313 1104 5 0.05635529409801876 1104 6 0.06640674028301419 1104 7 0.0800715498150737 1104 8 0.10005376381369047 1104 9 0.15589692694835122 1104 10-11 0.15368068463340023 1104 12-13 0.2045084720200805 1104 14-15 0.21247147935694954 1104 16-17 0.17871991749385074 1104 18-19 0.11527131493325271 1104 20-21 0.2074086401493176 1104 22-23 0.2020645500382372 1104 24-25 0.22379542475406566 1104 26-27 0.19365664152963546 1104 28-29 0.26907264246246854 1104 30-31 0.26855806052920883 1104 32-33 0.17721169289686856 1104 34-35 0.15725496477876533 1104 36-37 0.14258753661717805 1104 38-39 0.2528454458738878 1104 40-41 0.26010579764909636 1104 42-43 0.2795487005595163 1104 44-45 0.3034989295268957 1104 46-47 0.3706169007416449 1104 48-49 0.18827045858584057 1104 50-51 0.11587279274775852 1104 52-53 0.14704308359800677 1104 54-55 0.2582907063757247 1104 56-57 0.2601047845204363 1104 58-59 0.3139873192984197 1104 60-61 0.22313368177222515 1104 62-63 0.177904524022793 1104 64-65 0.07653064493518968 1104 66-67 0.07407240751344801 1104 68-69 0.1882448571594253 1104 70-71 0.24023398751416636 1104 72-73 0.3460587525697605 1104 74-75 0.2982654327188925 1104 76-77 0.27355657553447443 1104 78-79 0.3595912133073895 1104 80-81 0.43014061421044403 1104 82-83 0.4413736375823305 1104 84-85 0.42800693438440973 1104 86-87 0.4205286821180607 1104 88-89 0.6297996454329642 1104 90-91 0.7166086522612645 1104 92-93 0.6157621813788339 1104 94-95 0.5280065335490356 1104 96-97 0.5221632126437541 1104 98-99 0.5123614068803022 1104 100-101 0.47473962592244234 1105 1 0.07749811551893515 1105 2 0.04515716347013665 1105 3 0.09379898534585607 1105 4 0.1096167601508995 1105 5 0.13479003214089857 1105 6 0.12438223623264122 1105 7 0.12282671809553847 1105 8 0.12167332038931988 1105 9 0.19683927984834781 1105 10-11 0.15678589698382694 1105 12-13 0.13782391275788797 1105 14-15 0.24952403652084598 1105 16-17 0.1745950551580009 1105 18-19 0.13427443140949435 1105 20-21 0.14291618085250946 1105 22-23 0.20828670795498283 1105 24-25 0.2356995433273923 1105 26-27 0.31638460484047215 1105 28-29 0.25676845475032195 1105 30-31 0.268293090990646 1105 32-33 0.22387180952369334 1105 34-35 0.3122730064420054 1105 36-37 0.32418131230034675 1105 38-39 0.3972426475488291 1105 40-41 0.4425998127032855 1105 42-43 0.23963813314253457 1105 44-45 0.29416746939589444 1105 46-47 0.36478086619851524 1105 48-49 0.46252626763398297 1105 50-51 0.4863633658798179 1105 52-53 0.4949355499481527 1105 54-55 0.4969179013419449 1105 56-57 0.4028228624292538 1105 58-59 0.41216657896869435 1105 60-61 0.2812395169887978 1105 62-63 0.38072100539628195 1105 64-65 0.3370838010294719 1105 66-67 0.44948838354068243 1105 68-69 0.539468618168705 1105 70-71 0.5719129597541794 1105 72-73 0.5570819144743311 1105 74-75 0.5226771326258657 1105 76-77 0.5497810170702699 1105 78-79 0.7726709464766266 1105 80-81 0.7593989904862255 1105 82-83 0.9568906479964632 1105 84-85 0.7698277632160995 1105 86-87 0.7853442674761304 1105 88-89 0.8960898077281492 1105 90-91 0.977093219327724 1105 92-93 0.8773740087822901 1105 94-95 0.7759158420925196 1105 96-97 0.6967532641371506 1105 98-99 0.8379817487097796 1105 100-101 0.66905133603397 1106 1 0.10613396915666584 1106 2 0.08852843412096689 1106 3 0.10509792275168905 1106 4 0.07349683479546343 1106 5 0.06475203407914876 1106 6 0.10601380664117954 1106 7 0.09381817315073704 1106 8 0.07223724220032324 1106 9 0.11847400389606122 1106 10-11 0.15894117903837213 1106 12-13 0.12533780484218227 1106 14-15 0.15646542160625643 1106 16-17 0.1318844415423257 1106 18-19 0.02432258641668028 1106 20-21 0.185173300648529 1106 22-23 0.10804160489096404 1106 24-25 0.1193645529414411 1106 26-27 0.16044069102239433 1106 28-29 0.16499932644807558 1106 30-31 0.24141046837773672 1106 32-33 0.22330650831192855 1106 34-35 0.2303260974260084 1106 36-37 0.1956608347796447 1106 38-39 0.18227177062200184 1106 40-41 0.12943040786282722 1106 42-43 0.1893461304525772 1106 44-45 0.15390057179982364 1106 46-47 0.19042682438976044 1106 48-49 0.1122773680541016 1106 50-51 0.1430207083035313 1106 52-53 0.15584233257559532 1106 54-55 0.2216135536479129 1106 56-57 0.23486999946398868 1106 58-59 0.24867601419646945 1106 60-61 0.031002677674308643 1106 62-63 0.05689465398216953 1106 64-65 0.06771600191274274 1106 66-67 0.0037269910191568556 1106 68-69 0.1574321240334342 1106 70-71 0.26012257602513245 1106 72-73 0.35681595588317805 1106 74-75 0.4189515103705972 1106 76-77 0.4044357100321143 1106 78-79 0.39707441892189266 1106 80-81 0.5049761760581504 1106 82-83 0.4830702191470948 1106 84-85 0.40227339521070604 1106 86-87 0.5616898096451735 1106 88-89 0.7621841622837522 1106 90-91 0.6510214186073462 1106 92-93 0.7175089268319645 1106 94-95 0.8382027468209792 1106 96-97 0.6540665685714089 1106 98-99 0.7079942974602105 1106 100-101 0.7430463793028608 1107 1 0.027171331713837787 1107 2 0.042239879730416874 1107 3 0.016171392597030376 1107 4 0.055781073883757415 1107 5 0.0896614057700802 1107 6 0.08263697219961585 1107 7 0.05487902789074184 1107 8 0.07580800117835196 1107 9 0.07539071851453372 1107 10-11 0.02434616713023985 1107 12-13 0.09332460447277668 1107 14-15 0.1037656690351696 1107 16-17 0.051148427565124166 1107 18-19 0.15671295562997045 1107 20-21 0.15614691551356685 1107 22-23 0.1261036510442537 1107 24-25 0.033237351869622955 1107 26-27 0.15551911952835695 1107 28-29 0.1579762776543845 1107 30-31 0.12168051565257088 1107 32-33 0.1457448557355221 1107 34-35 0.1823380446869507 1107 36-37 0.2625055892844159 1107 38-39 0.1604828070507338 1107 40-41 0.10329745497514153 1107 42-43 0.2055446578820863 1107 44-45 0.2697735550933871 1107 46-47 0.21417701073563222 1107 48-49 0.328693341010009 1107 50-51 0.15460025872565808 1107 52-53 0.16391317929522842 1107 54-55 0.07432433693432472 1107 56-57 0.10033362198930718 1107 58-59 0.00868167719046653 1107 60-61 -0.06439440527162787 1107 62-63 -0.008486040129497496 1107 64-65 0.1937946174247358 1107 66-67 0.08500757904871392 1107 68-69 0.018475298421243735 1107 70-71 0.027825793849238778 1107 72-73 -0.07227778983327227 1107 74-75 -0.060768417486848136 1107 76-77 0.02975543873653308 1107 78-79 0.2878962636271858 1107 80-81 0.18968658437226438 1107 82-83 0.1716033284572802 1107 84-85 0.17257429247957745 1107 86-87 0.178994985487261 1107 88-89 0.3886608557866218 1107 90-91 0.42253191363080234 1107 92-93 0.48937464929820607 1107 94-95 0.4054272147960134 1107 96-97 0.39914739766829754 1107 98-99 0.5957115179311998 1107 100-101 0.47859782264615447 1108 1 -0.028998008608787984 1108 2 -0.040846309665660385 1108 3 0.035046796899678156 1108 4 -0.10662355628438291 1108 5 -0.010935254976267572 1108 6 0.010672695041535007 1108 7 -0.020613888089833665 1108 8 0.001887687507135638 1108 9 0.032801561941006696 1108 10-11 0.030800507541620448 1108 12-13 -0.032236871399696554 1108 14-15 -0.0017098294031967498 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-1.051528611566205 2316 48-49 -1.0102434800928748 2316 50-51 -1.0282275004287342 2316 52-53 -1.0688908208992416 2316 54-55 -0.9969839518629016 2316 56-57 -0.8337154925251156 2316 58-59 -0.7298331249754 2316 60-61 -0.682626146207653 2316 62-63 -0.7862097088965854 2316 64-65 -1.0180186966769895 2316 66-67 -0.8869624435667376 2316 68-69 -0.7383824740406126 2316 70-71 -0.7273593534765368 2316 72-73 -0.854395338051539 2316 74-75 -0.9146104242119577 2316 76-77 -0.8627596472391197 2316 78-79 -0.8854288049296137 2316 80-81 -0.9915747667259183 2316 82-83 -0.9582247409318612 2316 84-85 -1.121676076835186 2316 86-87 -1.1482661382245603 2316 88-89 -1.2964516755436541 2316 90-91 -1.458478531942088 2316 92-93 -1.2994702157430957 2316 94-95 -1.119658964918397 2316 96-97 -1.0562058820320743 2316 98-99 -1.0404173542785102 2316 100-101 -0.3558156742862444 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 9.0 4 30.0 5 64.0 6 181.0 7 344.0 8 830.0 9 1557.0 10 2560.0 11 4049.0 12 5729.0 13 8414.0 14 10025.0 15 13291.0 16 17650.0 17 23722.0 18 22075.0 19 15473.0 20 14555.0 21 15083.0 22 16141.0 23 18057.0 24 21357.0 25 25460.0 26 30261.0 27 37013.0 28 45465.0 29 55393.0 30 68363.0 31 83820.0 32 103165.0 33 132739.0 34 181580.0 35 260067.0 36 409264.0 37 678813.0 38 601560.0 39 52373.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62560565010656 19.444408883474637 14.51980225705101 23.410183209367794 2 15.652210722658335 20.767482222635827 39.50374562663067 24.076561428075166 3 19.070544370674924 24.813692954701068 29.847863524671954 26.267899149952058 4 13.09239122766113 16.7331980979245 36.63067630918873 33.54373436522564 5 13.696146328385527 37.86052790187796 33.88056863376177 14.562757135974746 6 32.305786117966576 36.042241019600624 17.26566232314185 14.386310539290948 7 28.94735427533598 32.90191490919988 20.44126431444853 17.70946650101561 8 26.558639600771095 35.003047164188736 19.78869411376572 18.649619121274448 9 26.891240763598518 16.851321904843815 20.22077326074789 36.036664070809785 10-11 25.12294484397646 26.732986442639067 27.11242053687875 21.031648176505726 12-13 25.93529798712464 23.937507265151662 27.813772241442912 22.313422506280787 14-15 22.627846600038467 25.41170027893804 26.0779094681867 25.88254365283679 16-17 22.242253074438114 28.884054010061078 26.685345081730528 22.18834783377028 18-19 22.202404269658242 27.046656216046966 29.17977193639053 21.571167577904262 20-21 22.76708713276968 26.42479282979085 29.093094057593085 21.715025979846388 22-23 22.379589980037213 26.341709731238105 29.039978659771855 22.23872162895283 24-25 22.363514257167218 26.59620001491666 29.084695038329766 21.95559068958636 26-27 22.019936133149447 26.897115912790337 28.96276001552156 22.120187938538656 28-29 21.951710342686706 26.99569096715715 29.182694795094164 21.86990389506198 30-31 22.109124761719823 26.708444508351647 29.032133975779917 22.150296754148613 32-33 21.8016198684512 26.836798320733685 28.935394071905012 22.4261877389101 34-35 22.570890056577657 26.829417021676903 28.59684724032737 22.00284568141807 36-37 22.674810705533314 26.460061580977168 28.58427934362461 22.280848369864906 38-39 22.187785691702764 26.72646404345756 28.831658059307287 22.254092205532388 40-41 22.356179444732955 26.538298244467434 28.97503235476311 22.1304899560365 42-43 22.350674114853106 26.64575091020666 28.464148365239772 22.53942660970046 44-45 22.2037293519535 26.976916855155643 28.394544304637375 22.42480948825349 46-47 22.44475744847402 27.06681348179467 27.903542124939 22.584886944792302 48-49 22.54788952803559 27.090650278948225 27.830536683892493 22.53092350912369 50-51 22.558052341344208 27.197922552979332 27.729782048809724 22.514243056866736 52-53 22.83214593598335 27.126110938277957 27.658793537996157 22.382949587742537 54-55 22.202118703003286 27.062144007568524 27.865997343222226 22.86973994620596 56-57 22.38594339899968 27.533333613300687 27.489037178265015 22.59168580943462 58-59 22.451998111219346 26.903735179648336 27.814579659794386 22.829687049337927 60-61 22.40863947546042 27.06075000747528 28.0846282366704 22.445982280393903 62-63 22.340958073169954 27.167053867048324 27.957312207814894 22.534675851966828 64-65 22.046018234273884 26.922489637708242 27.981152597607117 23.050339530410753 66-67 22.01497175577802 27.337799819790643 27.698420110880495 22.948808313550842 68-69 22.261216278596294 26.96746404024563 27.942830592214673 22.828489088943403 70-71 22.090351976138713 27.134015132350108 27.764008980906336 23.01162391060484 72-73 22.23778093879534 27.088029784938072 27.658423981148566 23.015765295118022 74-75 22.06649133993922 27.352800468194932 27.4404358351884 23.140272356677453 76-77 22.071633159182984 27.04359786574478 27.77389111957813 23.110877855494106 78-79 22.090546131109363 27.105650927352514 27.696320356064664 23.10748258547346 80-81 22.012899367630297 27.47770243767643 27.543265963996333 22.966132230696935 82-83 22.225803937325846 27.27905707906299 27.432759131581726 23.062379852029437 84-85 21.969684243910205 27.14317774542102 27.377963929907835 23.50917408076094 86-87 22.049907153665853 27.033694567762183 27.489661460220688 23.42673681835127 88-89 21.970010005656103 26.96724095929544 27.4393022508933 23.623446784155156 90-91 21.8515148031024 27.11081649628721 27.589131005778817 23.44853769483157 92-93 21.993604650772138 26.73202895444305 27.658306394878885 23.616059999905932 94-95 21.67675053258199 26.530159040522594 27.72834673313317 24.064743693762246 96-97 21.8421031413004 26.405861440371332 27.67201359431662 24.080021824011656 98-99 21.51917764866432 26.55279388336383 27.462878014659985 24.465150453311868 100-101 21.566464451403327 26.022373024168065 28.166546896370303 24.2446156280583 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 571.0 1 408.0 2 314.5 3 409.5 4 365.0 5 349.5 6 631.5 7 1301.5 8 2074.0 9 2360.0 10 2108.0 11 1938.5 12 2121.5 13 2375.5 14 2625.0 15 2827.5 16 3079.0 17 3332.5 18 3579.5 19 3838.0 20 4219.5 21 4900.5 22 5834.0 23 7126.0 24 8747.0 25 11177.5 26 14748.0 27 19217.0 28 23465.0 29 27853.0 30 33579.0 31 40002.0 32 48468.0 33 54655.0 34 59386.0 35 65458.0 36 72735.0 37 80304.5 38 83970.0 39 84762.0 40 85851.5 41 91118.0 42 96252.5 43 100751.0 44 105297.5 45 110150.0 46 110796.5 47 110401.5 48 120280.0 49 121752.0 50 113740.0 51 109910.5 52 105652.0 53 103821.0 54 102566.0 55 94286.5 56 82943.5 57 73799.0 58 66118.0 59 56627.5 60 46760.5 61 39109.5 62 33548.0 63 26221.0 64 18785.5 65 14574.0 66 10890.5 67 8934.5 68 6917.0 69 4567.5 70 3587.0 71 2941.5 72 2487.0 73 2220.0 74 1815.5 75 1561.5 76 1100.5 77 583.0 78 330.5 79 216.5 80 142.0 81 87.5 82 49.0 83 28.5 84 14.5 85 5.0 86 6.5 87 4.5 88 1.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2768316749189831 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023685234322558626 16-17 6.215274254855809E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011086705427580633 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0032924155512209148 36-37 0.020795971695977 38-39 0.0070215801041344 40-41 0.01836025610961459 42-43 0.005156997827677657 44-45 5.20739194325757E-4 46-47 1.8477842379301053E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.6798038526637322E-5 58-59 4.8714311727248236E-4 60-61 0.0020493607002497532 62-63 0.0014446313132908098 64-65 1.6798038526637322E-5 66-67 0.0 68-69 5.375372328523943E-4 70-71 3.3596077053274644E-5 72-73 0.0 74-75 0.0 76-77 0.2344166276392238 78-79 0.0 80-81 0.0011926607353912497 82-83 0.0 84-85 0.0 86-87 0.10335833105439944 88-89 0.35999876366436445 90-91 0.3597131970094116 92-93 0.0 94-95 3.3596077053274644E-5 96-97 0.0 98-99 0.0 100-101 2.351725393729225E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2976538.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.48640648433715 #Duplication Level Percentage of deduplicated Percentage of total 1 69.7488726586216 31.0287670091571 2 15.2914217271962 13.605208053589498 3 6.002588167178215 8.01100731489487 4 2.9183115219008 5.193007704448142 5 1.6427024694751362 3.653896489494767 6 0.9940022541808939 2.6531752995503197 7 0.6597652028134756 2.054540809760699 8 0.471790671199636 1.679061725960421 9 0.332317580847618 1.3305253485130835 >10 1.6811407808870547 13.573796692106047 >50 0.14707242104921367 4.576796051262684 >100 0.10033538585649063 8.299860296684228 >500 0.0071837506672559805 2.170649561214107 >1k 0.0023441712702911405 1.6518546950253743 >5k 1.5123685614781552E-4 0.5178529483386406 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8787 0.2952087290671243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6607 0.2219692810909856 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4686 0.15743121707164498 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.6798038526637322E-5 0.0 18-19 0.0 0.0 0.0 3.3596077053274644E-5 0.0 20-21 0.0 0.0 0.0 5.0394115579911966E-5 0.0 22-23 0.0 0.0 0.0 1.8477842379301053E-4 0.0 24-25 0.0 0.0 0.0 3.527588090593837E-4 0.0 26-27 0.0 0.0 0.0 8.566999648585035E-4 0.0 28-29 0.0 0.0 0.0 0.0027884743954217956 0.0 30-31 0.0 0.0 0.0 0.010162813308615579 0.0 32-33 0.0 0.0 0.0 0.024256367632464294 0.0 34-35 0.0 0.0 0.0 0.044985147174334744 0.0 36-37 0.0 0.0 0.0 0.0793539339998347 0.0 38-39 0.0 0.0 0.0 0.13193179458820953 0.0 40-41 0.0 0.0 0.0 0.1921023685906244 0.0 42-43 0.0 0.0 0.0 0.25427190917770914 0.0 44-45 0.0 0.0 0.0 0.33387781375544345 0.0 46-47 0.0 0.0 0.0 0.42751008050291983 0.0 48-49 0.0 0.0 0.0 0.5335728957601079 0.0 50-51 0.0 0.0 0.0 0.6505712340981369 0.0 52-53 0.0 0.0 0.0 0.7673343998967929 0.0 54-55 0.0 0.0 0.0 0.8974016122085456 0.0 56-57 0.0 0.0 0.0 1.0550680018195635 0.0 58-59 0.0 0.0 0.0 1.2290284888014198 0.0 60-61 0.0 0.0 0.0 1.4258679042565556 0.0 62-63 0.0 0.0 0.0 1.6265540705342918 0.0 64-65 0.0 0.0 0.0 1.8393684206282601 0.0 66-67 0.0 0.0 0.0 2.0953537297356863 0.0 68-69 0.0 0.0 0.0 2.3796773298375493 0.0 70-71 0.0 0.0 0.0 2.6917680876239443 0.0 72-73 0.0 0.0 0.0 3.0171124978078563 0.0 74-75 0.0 0.0 0.0 3.355257013349065 0.0 76-77 0.0 0.0 0.0 3.7305923861882495 0.0 78-79 0.0 0.0 0.0 4.15380216882835 0.0 80-81 0.0 0.0 0.0 4.609465761901914 0.0 82-83 0.0 0.0 0.0 5.08884818537509 0.0 84-85 0.0 0.0 0.0 5.576478445764845 0.0 86-87 0.0 0.0 0.0 6.1011651791443615 0.0 88-89 0.0 0.0 0.0 6.659599171923893 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8095 0.0 34.853104 1 GTATCAA 12695 0.0 34.779144 1 ATCAACG 15910 0.0 27.619267 3 TCAACGC 15995 0.0 27.472494 4 GTACATG 13585 0.0 27.45744 1 CAACGCA 16165 0.0 27.18358 5 TATCAAC 16450 0.0 26.799158 2 AACGCAG 16660 0.0 26.375902 6 TACATGG 14105 0.0 25.871675 2 ACATGGG 13840 0.0 25.338428 3 ACGCAGA 19000 0.0 23.102528 7 CGCAGAG 19185 0.0 22.78081 8 CATGGGG 5910 0.0 21.358242 4 GCAGAGT 20550 0.0 21.083605 9 GAGTACT 9745 0.0 20.915804 12-13 GCACCGT 755 0.0 20.741413 6 AGTACTT 10155 0.0 20.4452 12-13 CATGGGA 7760 0.0 20.057806 4 AGAGTAC 18190 0.0 19.644993 10-11 GTACTTT 10260 0.0 19.588415 14-15 >>END_MODULE