##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939267_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1194440 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.054746994407424 33.0 31.0 34.0 30.0 34.0 2 32.041464619403236 33.0 31.0 34.0 30.0 34.0 3 32.08303807642075 34.0 31.0 34.0 30.0 34.0 4 35.56479019456817 37.0 35.0 37.0 33.0 37.0 5 35.62681340209638 37.0 35.0 37.0 33.0 37.0 6 35.7715573825391 37.0 35.0 37.0 35.0 37.0 7 35.735378085127756 37.0 35.0 37.0 35.0 37.0 8 35.74427179263923 37.0 35.0 37.0 35.0 37.0 9 37.34311895114028 39.0 37.0 39.0 34.0 39.0 10-11 37.25885519574027 39.0 37.0 39.0 34.0 39.0 12-13 37.177120659053614 39.0 37.0 39.0 34.0 39.0 14-15 38.25024279160108 40.0 38.0 41.0 33.5 41.0 16-17 38.22173152272194 40.0 38.0 41.0 33.5 41.0 18-19 38.30649760557249 40.0 38.0 41.0 34.0 41.0 20-21 38.3051136934463 40.0 38.0 41.0 34.0 41.0 22-23 38.31113659957805 40.0 38.0 41.0 34.0 41.0 24-25 38.21622726968286 40.0 38.0 41.0 34.0 41.0 26-27 38.019435886273065 40.0 37.5 41.0 33.5 41.0 28-29 37.83097183617427 40.0 37.5 41.0 33.0 41.0 30-31 37.62439176517866 40.0 37.0 41.0 33.0 41.0 32-33 37.32106342721275 40.0 36.0 41.0 33.0 41.0 34-35 37.041647131710256 40.0 35.0 41.0 31.5 41.0 36-37 36.73669543886675 39.0 35.0 41.0 31.0 41.0 38-39 36.05313954656575 38.5 35.0 40.5 30.0 41.0 40-41 35.97444995144168 38.5 35.0 40.0 30.0 41.0 42-43 36.118998024178694 39.0 35.0 41.0 30.0 41.0 44-45 35.93481380395834 39.0 35.0 41.0 29.5 41.0 46-47 35.47644544723887 39.0 35.0 41.0 26.5 41.0 48-49 35.129610110177154 39.0 35.0 41.0 24.5 41.0 50-51 33.950453769130306 38.0 34.0 40.0 23.0 40.5 52-53 33.99021047520176 38.0 33.5 39.5 23.0 40.5 54-55 34.50250577676569 38.0 35.0 40.0 23.0 41.0 56-57 34.496751197213754 38.0 35.0 40.0 23.0 41.0 58-59 34.333515706104954 38.0 35.0 40.0 22.0 41.0 60-61 33.976185074177025 38.0 34.0 40.0 21.0 41.0 62-63 33.49176266702388 37.5 33.5 40.0 19.0 41.0 64-65 32.99376067445833 36.5 33.0 39.5 14.0 41.0 66-67 32.597635712132885 36.0 33.0 39.0 11.5 41.0 68-69 32.22553790897827 36.0 33.0 39.0 7.5 40.0 70-71 31.72070342587321 35.0 32.0 38.0 4.0 40.0 72-73 31.174095810589066 35.0 31.0 37.0 2.0 39.0 74-75 30.710441294665284 35.0 31.0 37.0 2.0 39.0 76-77 30.151101352935267 35.0 30.5 36.0 2.0 39.0 78-79 29.75263847493386 34.5 30.0 36.0 2.0 37.5 80-81 29.423073992833462 34.0 30.0 35.0 2.0 37.0 82-83 29.0080979371086 34.0 29.0 35.0 2.0 37.0 84-85 28.583956079836575 34.0 29.0 35.0 2.0 36.0 86-87 28.09769641003315 34.0 29.0 35.0 2.0 36.0 88-89 27.55536862462744 34.0 26.5 35.0 2.0 35.5 90-91 27.045540169451794 33.5 25.5 35.0 2.0 35.0 92-93 26.63873698134691 33.0 25.0 35.0 2.0 35.0 94-95 26.198931716955226 33.0 24.5 35.0 2.0 35.0 96-97 25.495217005458628 33.0 20.5 35.0 2.0 35.0 98-99 24.69245420448076 32.5 8.5 35.0 2.0 35.0 100-101 22.133836777067078 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34516869885965207 1101 2 0.43651740251539195 1101 3 0.4082254028461918 1101 4 0.4114515948423474 1101 5 0.2880761055637464 1101 6 0.3763952733296705 1101 7 0.3055455208041238 1101 8 0.29125508958004076 1101 9 0.27422426926766263 1101 10-11 0.46318173641518 1101 12-13 0.4605510529481478 1101 14-15 0.5795433379845747 1101 16-17 0.7881337769696373 1101 18-19 0.743832260018813 1101 20-21 0.6380358480532209 1101 22-23 0.74458021623348 1101 24-25 0.6621357675390911 1101 26-27 0.736524099308852 1101 28-29 0.6560733754682886 1101 30-31 0.6922205670119084 1101 32-33 0.8686288016866399 1101 34-35 0.8626964629039691 1101 36-37 0.7152224329558265 1101 38-39 1.0803766715613818 1101 40-41 0.9012747232321558 1101 42-43 0.866861415829085 1101 44-45 0.7651792994407245 1101 46-47 0.8561790170051253 1101 48-49 0.8419313599647964 1101 50-51 0.8982489834097436 1101 52-53 0.9186046285931866 1101 54-55 0.8777247393664922 1101 56-57 0.8281415750366605 1101 58-59 0.8924554603811927 1101 60-61 0.8853257908934538 1101 62-63 1.0061152468255088 1101 64-65 1.0006330963922565 1101 66-67 0.7782764639060744 1101 68-69 0.7554041073050541 1101 70-71 0.7299183671158573 1101 72-73 0.7356693142792423 1101 74-75 0.5842378923653655 1101 76-77 0.6790516979282835 1101 78-79 0.7558512561580102 1101 80-81 0.7618769888080159 1101 82-83 0.7664109356550135 1101 84-85 0.5901980401935205 1101 86-87 0.4870393987420094 1101 88-89 0.6930013576609788 1101 90-91 0.5818027815360729 1101 92-93 0.5732559565061841 1101 94-95 0.9144346559359029 1101 96-97 1.055970200349691 1101 98-99 1.0151476617024429 1101 100-101 0.6398683132199352 1102 1 0.3109716804326226 1102 2 0.2959071843961141 1102 3 0.3350516979984235 1102 4 0.34490988912538256 1102 5 0.33351784281538954 1102 6 0.4129931673077891 1102 7 0.3586483300405163 1102 8 0.3556361839658919 1102 9 0.5536462571537513 1102 10-11 0.621142211184349 1102 12-13 0.5322650643271345 1102 14-15 0.7405503983960102 1102 16-17 0.6453496976541757 1102 18-19 0.5493109908937299 1102 20-21 0.45495495409365816 1102 22-23 0.583819603312385 1102 24-25 0.5918933745074639 1102 26-27 0.6121361326596428 1102 28-29 0.5762703214627223 1102 30-31 0.5074803482831314 1102 32-33 0.5726316742573943 1102 34-35 0.4920023378058147 1102 36-37 0.4280823986651683 1102 38-39 0.9670161259496055 1102 40-41 0.7967818706510386 1102 42-43 0.8414269748364731 1102 44-45 1.0083646730030082 1102 46-47 0.9515365292811282 1102 48-49 0.6787221719783645 1102 50-51 0.6958100365796867 1102 52-53 0.824561061270181 1102 54-55 0.7300170101902026 1102 56-57 0.7856501410744343 1102 58-59 0.8538855448181621 1102 60-61 0.7644662633129755 1102 62-63 0.5911095979663727 1102 64-65 0.691579551920853 1102 66-67 0.7866799699970457 1102 68-69 0.5288830890441005 1102 70-71 0.676455161549498 1102 72-73 0.7940729528688593 1102 74-75 0.5031253100284943 1102 76-77 0.5630610540996734 1102 78-79 0.623349955462924 1102 80-81 0.8683387713388626 1102 82-83 0.7828129436278992 1102 84-85 0.7448994898170902 1102 86-87 0.6165214968493125 1102 88-89 0.8323444470171886 1102 90-91 0.826741112247408 1102 92-93 0.7399635505338509 1102 94-95 0.6270234410734368 1102 96-97 0.6638647349570164 1102 98-99 0.6723294860675288 1102 100-101 0.5523489794584826 1103 1 0.34555992820896364 1103 2 0.20208833061810338 1103 3 0.30682231213433653 1103 4 0.35296102880461433 1103 5 0.2521505814053313 1103 6 0.2671106895443529 1103 7 0.24522151177028206 1103 8 0.3403597078646783 1103 9 0.477660863438345 1103 10-11 0.5162408831795418 1103 12-13 0.4404223029259171 1103 14-15 0.5327202981325314 1103 16-17 0.5315851311566817 1103 18-19 0.4901458125591347 1103 20-21 0.5776344823071256 1103 22-23 0.4724939857282635 1103 24-25 0.47289813127568436 1103 26-27 0.43632314968851915 1103 28-29 0.4966629758808594 1103 30-31 0.495855443330079 1103 32-33 0.5551585084424602 1103 34-35 0.5876226628221701 1103 36-37 0.48124105191622135 1103 38-39 0.9861873165563111 1103 40-41 1.013930992682475 1103 42-43 0.9197133888698232 1103 44-45 0.8701354639704562 1103 46-47 0.904174855747506 1103 48-49 0.9943125240499455 1103 50-51 0.8580763979172445 1103 52-53 1.130844327702249 1103 54-55 1.1649266492770423 1103 56-57 1.1369998077469958 1103 58-59 1.108753274831777 1103 60-61 1.1956177902532588 1103 62-63 1.0528622637017193 1103 64-65 0.9233155070763743 1103 66-67 1.075844617837042 1103 68-69 1.0785484353686527 1103 70-71 0.9261518954710937 1103 72-73 0.825713861075613 1103 74-75 0.8232390127039544 1103 76-77 1.0411998138033027 1103 78-79 1.1294957353783985 1103 80-81 0.7974753029651858 1103 82-83 0.7289894038142783 1103 84-85 0.8390083473085603 1103 86-87 0.8914171662450805 1103 88-89 0.7536650249026344 1103 90-91 0.6286818383176787 1103 92-93 0.45936020134462296 1103 94-95 0.6641465688695867 1103 96-97 0.6872400952512621 1103 98-99 0.8088248619229539 1103 100-101 0.34582478006324635 1104 1 0.20894660124454845 1104 2 0.3011346986279335 1104 3 0.33401873644086066 1104 4 0.34363965994180745 1104 5 0.28685323246814676 1104 6 0.23884974580693097 1104 7 0.38733144387304463 1104 8 0.28647182359704715 1104 9 0.39635307402212305 1104 10-11 0.4004138334678444 1104 12-13 0.5768633278670663 1104 14-15 0.5175713677698468 1104 16-17 0.5136043061541216 1104 18-19 0.44577036474447596 1104 20-21 0.46411645030330817 1104 22-23 0.6216265047497274 1104 24-25 0.6034877112681869 1104 26-27 0.6027856697335636 1104 28-29 0.5569558893204203 1104 30-31 0.4341883786059313 1104 32-33 0.5395447038090708 1104 34-35 0.5100927232131767 1104 36-37 0.525721416309409 1104 38-39 1.0142213675304177 1104 40-41 1.0222475507363242 1104 42-43 0.7325584194065229 1104 44-45 0.7996195814770246 1104 46-47 0.7134109265789945 1104 48-49 0.8197057479743322 1104 50-51 0.5856199852112383 1104 52-53 0.7361148930575965 1104 54-55 0.8347490587758344 1104 56-57 0.8380532384338082 1104 58-59 0.8432640053241229 1104 60-61 0.9767106286305491 1104 62-63 1.0763388408623271 1104 64-65 0.9446195433792113 1104 66-67 0.9934654851474249 1104 68-69 0.8453820018499414 1104 70-71 0.8851269543267364 1104 72-73 0.7016504334595197 1104 74-75 0.5798496101313653 1104 76-77 0.578269485235289 1104 78-79 0.5982186532534541 1104 80-81 0.5380618122285519 1104 82-83 0.5715710340302564 1104 84-85 0.6993737640747391 1104 86-87 0.6648698599569087 1104 88-89 0.7697129965154481 1104 90-91 0.6761689583149497 1104 92-93 0.4763549811051071 1104 94-95 0.5095899480363961 1104 96-97 0.5301347692421885 1104 98-99 0.5211128963151204 1104 100-101 0.5222159909540984 1105 1 0.3757079584247407 1105 2 0.4654449242294305 1105 3 0.4188408751911439 1105 4 0.4267119180021339 1105 5 0.39426636339103993 1105 6 0.37335031557328335 1105 7 0.39531466579622787 1105 8 0.4450275714194376 1105 9 0.5255098522937303 1105 10-11 0.465549002823181 1105 12-13 0.4734174589165647 1105 14-15 0.47543057217474427 1105 16-17 0.5331094697630974 1105 18-19 0.4643031920517018 1105 20-21 0.46689466997015217 1105 22-23 0.5751946056259598 1105 24-25 0.6238686268551064 1105 26-27 0.5500692964992666 1105 28-29 0.6526409237823572 1105 30-31 0.7975101975728194 1105 32-33 0.7606943981480967 1105 34-35 0.6348204203507066 1105 36-37 0.5932062631218002 1105 38-39 0.9866225999139218 1105 40-41 0.8868858383855382 1105 42-43 0.9191739423741083 1105 44-45 0.7733145568196136 1105 46-47 0.8203565569093954 1105 48-49 0.740698147843176 1105 50-51 0.5799328729688966 1105 52-53 0.482225547850625 1105 54-55 0.8552801264627803 1105 56-57 0.8100486543307781 1105 58-59 0.9682084084654647 1105 60-61 1.1445676903244006 1105 62-63 1.1127154190826403 1105 64-65 1.0766673583009307 1105 66-67 0.9713833868489345 1105 68-69 1.0612853006509937 1105 70-71 1.069775078967588 1105 72-73 1.076151684997562 1105 74-75 1.0698007822917077 1105 76-77 1.2149752617894833 1105 78-79 1.0221467295859092 1105 80-81 1.0519009660524787 1105 82-83 0.9852993187900623 1105 84-85 1.2579152271568041 1105 86-87 1.204325773677077 1105 88-89 0.9725481928170012 1105 90-91 1.1346165966253245 1105 92-93 1.1990759968800049 1105 94-95 0.9334514704650623 1105 96-97 1.3187684987606296 1105 98-99 1.3555560024669724 1105 100-101 1.0612019691828571 1106 1 0.2603176338430657 1106 2 0.268148235046894 1106 3 0.21142845036774816 1106 4 0.21127753043851527 1106 5 0.29254337579585865 1106 6 0.23807149221114798 1106 7 0.172406758990995 1106 8 0.26646936843908975 1106 9 0.292008860876642 1106 10-11 0.34798834200345397 1106 12-13 0.4162601931339722 1106 14-15 0.5492153635708945 1106 16-17 0.48861913940010737 1106 18-19 0.5439817979114565 1106 20-21 0.5577394135845353 1106 22-23 0.5415372062481509 1106 24-25 0.4811842537447717 1106 26-27 0.531776127667726 1106 28-29 0.572785362862156 1106 30-31 0.4580506253587515 1106 32-33 0.4678533045736373 1106 34-35 0.2555459595362919 1106 36-37 0.2394027840212516 1106 38-39 0.7278814886830176 1106 40-41 0.723227017117182 1106 42-43 0.6344575470385294 1106 44-45 0.6334899806133123 1106 46-47 0.6337666254570209 1106 48-49 0.4897860284918707 1106 50-51 0.41765609806667214 1106 52-53 0.610755078642363 1106 54-55 0.5899143333994203 1106 56-57 0.5015132484747724 1106 58-59 0.4653361114247119 1106 60-61 0.5158412823793057 1106 62-63 0.3441816226192387 1106 64-65 0.41202102623167036 1106 66-67 0.4996364302416012 1106 68-69 0.5379080918377781 1106 70-71 0.5317777883722243 1106 72-73 0.5826185526039254 1106 74-75 0.595965912222816 1106 76-77 0.6845553978222227 1106 78-79 0.7853925775873734 1106 80-81 0.8231383077109022 1106 82-83 0.7200057921421141 1106 84-85 0.8460505661370732 1106 86-87 0.8117929647244786 1106 88-89 0.8142312268501861 1106 90-91 0.8595413223645139 1106 92-93 0.891964434722297 1106 94-95 0.9465258322158938 1106 96-97 0.9355561042500611 1106 98-99 1.0362884479780945 1106 100-101 0.39172277175413583 1107 1 -0.04243872978793917 1107 2 -0.09152836773474604 1107 3 0.032673445647667165 1107 4 -0.01784561091548653 1107 5 -0.07201985298829072 1107 6 -0.14435065597351837 1107 7 0.011907882450707064 1107 8 -0.06446671042490948 1107 9 0.038716412780921416 1107 10-11 0.09347515857773914 1107 12-13 -0.0933210869100023 1107 14-15 0.028222990126849368 1107 16-17 -0.01543290600402969 1107 18-19 0.1578171176571459 1107 20-21 0.04493993179828948 1107 22-23 -0.053160240782688106 1107 24-25 -0.17145666878182908 1107 26-27 -0.0462356028876485 1107 28-29 -0.013272240517132161 1107 30-31 0.10385793562155499 1107 32-33 0.0633123754883016 1107 34-35 -0.1670348068629437 1107 36-37 -0.0850047742943758 1107 38-39 0.49899306160767765 1107 40-41 0.09358846236759177 1107 42-43 -0.008897238516624384 1107 44-45 0.07557377176845392 1107 46-47 0.2536636164715489 1107 48-49 0.12907439838093637 1107 50-51 0.09126581206898265 1107 52-53 0.19304177789636157 1107 54-55 0.28917017491090036 1107 56-57 0.20556138811540592 1107 58-59 0.22176790905606225 1107 60-61 0.2006159529871141 1107 62-63 0.17739336142830808 1107 64-65 0.04533873651097764 1107 66-67 0.04866665864032882 1107 68-69 0.20040111857603904 1107 70-71 0.09748415307178249 1107 72-73 0.11275114959029864 1107 74-75 0.03432891093538615 1107 76-77 0.10687528869666352 1107 78-79 0.07161945528317659 1107 80-81 -0.15565523878868603 1107 82-83 0.06151580453001415 1107 84-85 0.05576022052121843 1107 86-87 0.22806223837774553 1107 88-89 0.17444210860208997 1107 90-91 0.15418548888563421 1107 92-93 0.3527140907571784 1107 94-95 0.3552853543567842 1107 96-97 0.4001440941009733 1107 98-99 0.5538945981554377 1107 100-101 0.3357509698927821 1108 1 0.01692562121274932 1108 2 0.2527039235880366 1108 3 0.33106436329011046 1108 4 0.18632924573957155 1108 5 0.16766352424752284 1108 6 0.12751574971856172 1108 7 0.17966393843776984 1108 8 0.17510685305227724 1108 9 0.21952580234074048 1108 10-11 0.3081696898525692 1108 12-13 0.3223115577506306 1108 14-15 0.5019329547239977 1108 16-17 0.515995239193245 1108 18-19 0.43531648571004666 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2316 58-59 -0.956123134451083 2316 60-61 -0.9903126270106952 2316 62-63 -1.3773448185500285 2316 64-65 -1.3322499495119011 2316 66-67 -1.4109071546630538 2316 68-69 -1.4428813813761465 2316 70-71 -1.1017147763613302 2316 72-73 -1.1684291399704563 2316 74-75 -1.1898723222565906 2316 76-77 -1.1349805614888844 2316 78-79 -1.1693774840500097 2316 80-81 -1.0578938548874142 2316 82-83 -1.0345955664180515 2316 84-85 -1.0823346201600934 2316 86-87 -1.1147220121413959 2316 88-89 -1.2610677117858593 2316 90-91 -1.0856989812295375 2316 92-93 -1.206483818646852 2316 94-95 -1.1956619107192168 2316 96-97 -1.107914425431133 2316 98-99 -1.3802205063308932 2316 100-101 -0.991577231998793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4348.0 3 816.0 4 709.0 5 826.0 6 1014.0 7 1227.0 8 1563.0 9 2027.0 10 2464.0 11 3485.0 12 5128.0 13 7428.0 14 7879.0 15 8940.0 16 12092.0 17 16080.0 18 13695.0 19 8251.0 20 8048.0 21 8493.0 22 9233.0 23 10191.0 24 11314.0 25 12415.0 26 14268.0 27 16701.0 28 19629.0 29 23066.0 30 27660.0 31 33531.0 32 41056.0 33 53235.0 34 74167.0 35 119250.0 36 159776.0 37 232916.0 38 194440.0 39 27078.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.36266309353201 18.57920882268739 13.807025541700144 19.25110254208046 2 13.475215372641635 23.18216432731274 40.547757709132874 22.79486259091275 3 22.089857673630622 30.546828478692976 29.143357962980048 18.21995588469635 4 11.4339735964849 16.53063381522237 39.15313468342793 32.8822579048648 5 11.629865842475862 41.5204575332835 32.71519022917773 14.13448639506291 6 26.253356531533793 37.11904833740643 19.46168106497172 17.165914066088053 7 25.613243922883488 36.97544006705783 20.80620284995809 16.605113160100586 8 24.474516139038087 31.703068190296708 21.191965816408224 22.63044985425698 9 27.762300668762013 19.634205538057063 21.862238546077677 30.74125524710325 10-11 23.199617087391335 27.19572257780322 26.699017529065273 22.905642805740168 12-13 29.68986059337394 25.107986178774905 26.255843532668255 18.946309695182897 14-15 26.010183569144825 25.306269919909596 28.995676090743416 19.687870420202163 16-17 19.183054999511818 29.562106651726467 30.877391622454383 20.377446726307333 18-19 18.985618962229708 30.03519705446301 33.02265027071594 17.95653371259134 20-21 20.569408625571313 28.111615964472335 30.800969866305866 20.51800554365048 22-23 19.445489174984328 27.782858173112203 29.797671683469705 22.973980968433764 24-25 20.526819497555383 28.182217745013254 31.11424907168278 20.176713685748577 26-27 22.126219657929717 25.62378139033666 32.65483942655636 19.595159525177262 28-29 19.621010825087435 26.363157217235106 34.50564232052775 19.51018963714971 30-31 19.399106650980933 29.664361042469718 32.43182281268264 18.504709493866716 32-33 18.763663808465587 29.228327637895973 31.48330153671985 20.524707016918594 34-35 19.860734600202118 27.25546018990431 31.634406903961647 21.249398305931926 36-37 21.708123100652237 26.983174517339126 32.330636103575976 18.97806627843266 38-39 22.735802182669293 25.649769097095238 32.92255904144818 18.69186967878728 40-41 19.41704113151923 27.490014387352247 34.32356537334611 18.76937910778241 42-43 18.805620766431456 29.50704781956506 31.999177670301286 19.6881537437022 44-45 19.53161196687994 29.64824860058743 29.99901618584502 20.821123246687616 46-47 19.56765597473964 28.835702543274344 28.911887253356205 22.68475422862981 48-49 20.673218233095433 28.416644976219 29.638388349979966 21.271748440705604 50-51 21.82825317043287 29.17681220601412 29.87037395992016 19.12456066363285 52-53 19.412783779124286 29.59208130621444 30.972134035834276 20.023000878826995 54-55 17.905794865283696 29.162297765785432 31.545426338859663 21.38648103007121 56-57 20.27980962187037 30.730434234442473 28.25757782704168 20.732178316645477 58-59 19.686414517447982 28.691449601672815 29.670311660871825 21.951824220007378 60-61 19.83890662153767 27.831829803535136 30.771893170627006 21.55737040430019 62-63 22.566739326417203 27.285838749175852 29.913363339119204 20.234058585287745 64-65 21.157303692042735 28.136058300368667 31.36655364495035 19.340084362638247 66-67 19.46998329742195 28.561282313937824 31.27242358948999 20.69631079915024 68-69 19.10565002687365 30.473413592598853 30.374372212226962 20.046564168300534 70-71 19.5109714757191 30.044546979548254 28.515786149266237 21.92869539546641 72-73 19.588515816885838 28.798258122056765 28.169316725837984 23.443909335219416 74-75 20.124482549230365 29.549025148822288 29.007562644974527 21.318929656972823 76-77 21.195998790875738 27.553865098560866 30.491877299953206 20.75825881061019 78-79 19.793029348166034 27.676838926422764 31.830964719650527 20.699167005760675 80-81 19.233412597695043 30.784179130451594 29.98904142526206 19.993366846591304 82-83 19.135058463072603 29.8352322148283 28.87575088883293 22.153958433266165 84-85 19.67382795585007 28.236600855453283 28.84119460763417 23.24837658106248 86-87 20.95743155722732 28.141300340159077 29.7863484841669 21.114919618446702 88-89 22.027821784509406 26.334581218487536 30.48333686508377 21.15426013191929 90-91 20.04132066323323 27.505028412686865 31.471534537404956 20.98211638667495 92-93 19.240013070449642 29.575382808994387 30.288580220305207 20.896023900250768 94-95 19.174713901407898 29.818805276333833 29.2280836472256 21.778397175032666 96-97 19.54993298869576 28.30391802711319 28.183772877306023 23.962376106885028 98-99 20.660877609806356 27.927840037005108 28.3597064822018 23.05157587098673 100-101 21.93753670978573 27.6906880228451 29.21979118359707 21.1519840837721 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5714.0 1 3731.5 2 3288.0 3 4256.5 4 2910.0 5 2529.5 6 4188.5 7 7833.0 8 11063.5 9 10690.0 10 7741.0 11 5562.5 12 4982.0 13 4755.0 14 4621.5 15 4319.0 16 3963.0 17 3818.5 18 3678.5 19 3608.0 20 3706.5 21 3949.5 22 4269.5 23 4730.5 24 5185.0 25 5769.5 26 6725.0 27 7734.0 28 8489.0 29 9590.0 30 11369.0 31 13321.5 32 16066.0 33 19589.5 34 22499.0 35 24533.5 36 29197.5 37 34132.0 38 34860.5 39 34369.0 40 32897.5 41 30912.0 42 29473.5 43 29165.5 44 30994.0 45 32717.0 46 36324.0 47 49090.5 48 111936.0 49 114340.5 50 61006.5 51 44883.5 52 26356.5 53 20693.5 54 19252.0 55 17541.5 56 15844.5 57 14390.0 58 12983.0 59 11816.0 60 10593.5 61 9258.5 62 7662.0 63 5570.0 64 3915.0 65 3068.5 66 2502.5 67 1988.0 68 1400.5 69 953.5 70 773.0 71 652.0 72 578.0 73 603.5 74 506.0 75 332.0 76 246.0 77 181.5 78 138.5 79 122.0 80 92.0 81 58.0 82 44.5 83 29.5 84 21.0 85 16.5 86 9.5 87 7.0 88 4.5 89 1.5 90 1.5 91 2.0 92 2.5 93 2.0 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2787080137972606 2 0.17480995278121964 3 0.17665182009979571 4 0.19473560831854259 5 0.20787984327383544 6 0.10414922474130137 7 0.12055858812497908 8 0.13286561066273736 9 0.13721911523391714 10-11 0.1237818559324872 12-13 0.11281437326278423 14-15 0.12876326981681793 16-17 0.10368875791165735 18-19 0.1326563075583537 20-21 0.15944710491946015 22-23 0.16304711831485885 24-25 0.18661464786845716 26-27 0.16702387729814808 28-29 0.16564247680921604 30-31 0.15480057600214325 32-33 0.16451224004554435 34-35 0.3391547503432571 36-37 0.45046214125447914 38-39 0.4168480626904658 40-41 0.4063410468504069 42-43 0.4299922976457587 44-45 0.4345969659421988 46-47 0.4374434881618164 48-49 0.44878771641940995 50-51 0.442759787013161 52-53 0.4483272495897659 54-55 0.432252771173102 56-57 0.39369913934563483 58-59 0.38419677840661737 60-61 0.3411221995244634 62-63 0.44673654599645024 64-65 0.44271792639228424 66-67 0.45226214795217845 68-69 0.4640668430394159 70-71 0.4579970530122903 72-73 0.4502946987709722 74-75 0.45527611265530293 76-77 0.4311225344094304 78-79 0.4182713238002746 80-81 0.4152992197180269 82-83 0.42693647232175747 84-85 0.42940624895348445 86-87 0.4306202069589096 88-89 0.4376946518870768 90-91 0.4559877432102073 92-93 0.46034124778138713 94-95 0.4579970530122903 96-97 0.45724356183650916 98-99 0.45335052409497334 100-101 0.43648069388165167 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1194440.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.269425870115946 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71371793725375 22.111773610392444 2 13.5525964345929 8.475638191182007 3 5.62351700102205 5.275324439783871 4 2.9600379403253285 3.7023474779093424 5 1.6713912701585742 2.6131722711091236 6 1.0587358427118623 1.9863637169827992 7 0.7407878328341662 1.621480715700426 8 0.5408377211164719 1.3529348022571168 9 0.38954221056326194 1.0962685148840114 >10 2.3510891382420036 14.048321040187508 >50 0.22920164093140116 5.0240272561539925 >100 0.13768089254790586 7.72620194052454 >500 0.015029493141012592 3.119467460699571 >1k 0.011808887467938465 8.253400416721638 >5k 0.002683838060895106 6.230626635945178 >10k+ 0.001341919030447553 7.362651509566456 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 25890 2.1675429489970197 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21156 1.7712065905361507 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15547 1.3016141455410069 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14946 1.2512976792471786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10193 0.8533706171929942 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9538 0.7985332038444793 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9044 0.7571749104182712 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8948 0.7491376712099393 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8200 0.686514182378353 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6877 0.5757509795385285 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6594 0.5520578681223 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6525 0.5462811024413115 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6314 0.5286159204313319 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6198 0.5189042563879307 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6005 0.5027460567295133 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4730 0.3960014734938549 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4700 0.39348983624125117 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4048 0.3389035866179967 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4044 0.33856870165098285 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4001 0.3349686882555842 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3770 0.3156290814105355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3607 0.3019825190047219 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3337 0.2793777837312883 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3322 0.27812196510498643 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2967 0.24840092428250896 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.24304276481028766 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2604 0.21801011352600383 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2554 0.21382405143833094 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2459 0.20587053347175246 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2419 0.20252168380161414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2384 0.19959144034024315 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.19892167040621547 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2219 0.1857774354509226 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2202 0.1843541743411138 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2025 0.1695355145507518 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2007 0.16802853219918956 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1992 0.1667727135728877 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1954 0.16359130638625632 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1772 0.14835404038712702 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1741 0.14575868189276983 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1710 0.14316332339841265 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1668 0.13964703124476743 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1620 0.13562841164060147 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1603 0.13420515053079268 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1597 0.13370282308027193 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1593 0.1333679381132581 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1515 0.1268376812564884 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1426 0.11938649074043066 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1346 0.11268879140015405 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1322 0.11067948159807106 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1309 0.10959110545527612 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1271 0.10640969826864473 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1249 0.10456783095006865 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1239 0.10373061853253408 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1228 0.10280968487324604 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1216 0.10180502997220456 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.186062087672884E-5 0.0 12-13 0.0 0.0 0.0 1.6744248350691537E-4 0.0 14-15 0.0 0.0 0.0 1.6744248350691537E-4 0.0 16-17 0.0 0.0 0.0 4.186062087672885E-4 0.0 18-19 0.0 0.0 0.0 6.279093131509327E-4 0.0 20-21 0.0 0.0 0.0 0.0012139580054251365 0.0 22-23 0.0 0.0 0.0 0.0030558253240012055 0.0 24-25 0.0 0.0 0.0 0.005860486922742038 0.0 26-27 0.0 0.0 0.0 0.009083754730250158 0.0 28-29 0.0 0.0 0.0 0.017581460768226115 0.0 30-31 0.0 0.0 0.0 0.03842804996483708 0.0 32-33 0.0 0.0 0.0 0.07250259535849436 0.0 34-35 0.0 0.0 0.0 0.13198653762432605 0.0 36-37 0.0 0.0 0.0 0.22240547871806035 0.0 38-39 0.0 0.0 0.0 0.3295686681624862 0.0 40-41 0.0 0.0 0.0 0.4624761394461003 0.0 42-43 0.0 0.0 0.0 0.5801044841097083 0.0 44-45 0.0 0.0 0.0 0.7097886875858143 0.0 46-47 0.0 0.0 0.0 0.8789055959277987 0.0 48-49 0.0 0.0 0.0 1.0754830715649175 0.0 50-51 0.0 0.0 0.0 1.2651535447573758 0.0 52-53 0.0 0.0 0.0 1.4525635444224907 0.0 54-55 0.0 0.0 0.0 1.6408526171260172 0.0 56-57 0.0 0.0 0.0 1.8536301530424297 0.0 58-59 0.0 0.0 0.0 2.143180067646763 0.0 60-61 0.0 0.0 0.0 2.5345350122233015 0.0 62-63 0.0 0.0 0.0 2.881852583637521 0.0 64-65 0.0 0.0 0.0 3.177807173235993 0.0 66-67 0.0 0.0 0.0 3.5139060982552492 0.0 68-69 0.0 0.0 0.0 3.88935400689863 0.0 70-71 0.0 0.0 0.0 4.352625498141388 0.0 72-73 0.0 0.0 0.0 4.8325993771139615 0.0 74-75 0.0 0.0 0.0 5.3034895013562835 0.0 76-77 0.0 0.0 0.0 5.7449097485013905 0.0 78-79 0.0 0.0 0.0 6.258204681691839 0.0 80-81 0.0 0.0 0.0 6.836927765312615 0.0 82-83 0.0 0.0 0.0 7.437920699239811 0.0 84-85 0.0 0.0 0.0 8.202002612102742 0.0 86-87 0.0 0.0 0.0 9.026363819028163 0.0 88-89 0.0 0.0 0.0 9.717650112186464 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 60 3.3469405E-10 63.227165 2 ATCACGC 65 6.8030204E-10 58.36354 3 CCGTAAG 60 0.0039697024 31.636147 1 TACCTGG 945 0.0 28.100964 2 CGTAAGA 85 6.470483E-4 27.894339 2 CTTAACG 35 0.008462314 27.097355 20-21 TCGACCG 65 1.0158314E-5 25.534048 18-19 TCACGCA 135 1.3603514E-5 24.588343 4 CACGCAG 175 1.5105434E-7 24.38762 5 CATGGGG 2585 0.0 23.84777 4 ATGGGAG 2360 0.0 23.30832 5 CATGGGT 920 0.0 22.67931 4 GAGTACT 9020 0.0 22.64449 12-13 GCTTATG 345 0.0 22.007753 1 CGACCGT 65 2.9763635E-4 21.886328 18-19 AGTACTT 9325 0.0 21.878428 12-13 ATGGGTA 460 0.0 21.648434 5 TATAGGT 220 5.7407306E-8 21.554718 3 ATATGAG 420 0.0 21.452074 3 GATTGGC 355 0.0 21.358229 9 >>END_MODULE