##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939267_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1194440 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23804962995211 33.0 31.0 34.0 31.0 34.0 2 32.4023157295469 34.0 31.0 34.0 31.0 34.0 3 32.484482267840995 34.0 31.0 34.0 30.0 34.0 4 35.994992632530725 37.0 35.0 37.0 35.0 37.0 5 35.962840326847726 37.0 35.0 37.0 35.0 37.0 6 36.04760640969827 37.0 35.0 37.0 35.0 37.0 7 35.99012759117243 37.0 35.0 37.0 35.0 37.0 8 36.00242875322327 37.0 35.0 37.0 35.0 37.0 9 37.64507216771039 39.0 37.0 39.0 35.0 39.0 10-11 37.56662327115636 39.0 37.0 39.0 35.0 39.0 12-13 37.49322569572352 39.0 37.0 39.0 34.5 39.0 14-15 38.58062062556512 40.0 38.0 41.0 34.0 41.0 16-17 38.601798332272864 40.0 38.0 41.0 34.5 41.0 18-19 38.86402916848063 40.0 38.0 41.0 35.0 41.0 20-21 38.86829476574796 40.0 38.0 41.0 35.0 41.0 22-23 38.80305833696126 40.0 38.0 41.0 35.0 41.0 24-25 38.7048796925756 40.0 38.0 41.0 34.0 41.0 26-27 38.39822636549346 40.0 37.5 41.0 34.0 41.0 28-29 38.17272068919326 40.0 37.5 41.0 34.0 41.0 30-31 37.94304025317304 40.0 37.0 41.0 33.0 41.0 32-33 37.76288302468102 40.0 36.0 41.0 33.0 41.0 34-35 37.64853152941964 40.0 36.0 41.0 33.0 41.0 36-37 37.39441453735642 40.0 35.5 41.0 33.0 41.0 38-39 37.153726851076655 40.0 35.0 41.0 32.5 41.0 40-41 36.890078195639795 39.0 35.0 41.0 31.5 41.0 42-43 36.71182813703493 39.0 35.0 41.0 31.0 41.0 44-45 36.403100197582134 39.0 35.0 41.0 30.5 41.0 46-47 35.912619721375705 39.0 35.0 41.0 29.5 41.0 48-49 35.48641874016275 39.0 35.0 41.0 27.0 41.0 50-51 35.23328547269013 39.0 35.0 40.5 26.5 41.0 52-53 34.86139864706473 38.0 35.0 40.0 25.5 41.0 54-55 34.661911439670476 38.0 34.0 40.0 24.5 41.0 56-57 34.45260875389304 38.0 34.0 40.0 23.5 41.0 58-59 34.20907077793778 38.0 34.0 40.0 23.0 41.0 60-61 33.801864053447645 37.5 33.5 40.0 22.0 41.0 62-63 33.34971785941529 37.0 33.0 39.5 22.0 41.0 64-65 32.94716017547972 36.0 33.0 39.0 20.0 40.5 66-67 32.511506228860384 36.0 32.0 39.0 18.5 40.0 68-69 32.092766903318704 35.0 32.0 38.0 15.5 40.0 70-71 31.5709893339138 35.0 31.5 37.5 10.0 39.5 72-73 31.010049897860085 35.0 31.0 37.0 7.5 39.0 74-75 30.48500510699575 35.0 30.5 36.5 2.0 39.0 76-77 28.464168982954355 32.5 28.0 34.5 2.0 36.5 78-79 29.345566541642945 34.0 29.0 35.0 2.0 37.0 80-81 29.417249087438464 34.0 29.0 35.0 2.0 37.0 82-83 29.112635209805433 34.0 29.0 35.0 2.0 36.0 84-85 28.708940172800645 34.0 29.0 35.0 2.0 36.0 86-87 28.09027075449583 34.0 28.5 35.0 2.0 35.5 88-89 27.415907035933156 33.0 26.5 35.0 2.0 35.0 90-91 26.843814256053047 33.0 25.0 35.0 2.0 35.0 92-93 26.53507543283882 33.0 25.0 35.0 2.0 35.0 94-95 26.097467432436957 32.5 24.5 35.0 2.0 35.0 96-97 25.318113090653362 32.0 21.5 34.0 2.0 35.0 98-99 24.345547704363554 32.0 8.5 34.0 2.0 35.0 100-101 20.205175228558993 27.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3076362078343138 1101 2 0.34081719264662524 1101 3 0.27443435789101045 1101 4 0.2108111427647401 1101 5 0.20544553756054285 1101 6 0.24693479511375926 1101 7 0.26800036164080154 1101 8 0.20489759358717663 1101 9 0.43692592658649687 1101 10-11 0.3899404078683375 1101 12-13 0.4039425785287989 1101 14-15 0.41007218807677503 1101 16-17 0.4270371298731135 1101 18-19 0.45608598067843786 1101 20-21 0.489338482982717 1101 22-23 0.43706897523990307 1101 24-25 0.37992175514167315 1101 26-27 0.30870772254588275 1101 28-29 0.32103006577472826 1101 30-31 0.40623511900120235 1101 32-33 0.4213256754127812 1101 34-35 0.535883748633772 1101 36-37 0.42044522534973083 1101 38-39 0.5110085040752352 1101 40-41 0.5339363173039189 1101 42-43 0.5054921662837017 1101 44-45 0.719162571487189 1101 46-47 0.6532918300279746 1101 48-49 0.5465677260726167 1101 50-51 0.3336946721893099 1101 52-53 0.3678743202656136 1101 54-55 0.28249780862935125 1101 56-57 0.29094088293678766 1101 58-59 0.3475903103951907 1101 60-61 0.37225308092941134 1101 62-63 0.22000089530916966 1101 64-65 0.23839593221924815 1101 66-67 0.21314644544508354 1101 68-69 0.2732005859337363 1101 70-71 0.10544765771240705 1101 72-73 0.2956240234693084 1101 74-75 -0.01650064237570703 1101 76-77 0.10251585388021311 1101 78-79 0.3810938591555697 1101 80-81 0.6031032848068243 1101 82-83 0.5859296960328066 1101 84-85 0.6376057787451934 1101 86-87 0.64068305993192 1101 88-89 0.4911411299179811 1101 90-91 0.2782733209998902 1101 92-93 0.5092569158019238 1101 94-95 0.38788989103136373 1101 96-97 0.360475205755705 1101 98-99 0.3597581044854685 1101 100-101 0.6184218586728889 1102 1 0.19107537814543463 1102 2 0.24569994459060496 1102 3 0.2710309092161296 1102 4 0.20494321918642555 1102 5 0.26351439622600026 1102 6 0.28925044753269447 1102 7 0.2917891693914427 1102 8 0.29084946831059 1102 9 0.3155386127139792 1102 10-11 0.3015439330391132 1102 12-13 0.33875942564390016 1102 14-15 0.40156577470042265 1102 16-17 0.43796561858466987 1102 18-19 0.3281898914202799 1102 20-21 0.41791022702712155 1102 22-23 0.35131849846559504 1102 24-25 0.37329196432171585 1102 26-27 0.4138988430163053 1102 28-29 0.32900162626433627 1102 30-31 0.4239634384678155 1102 32-33 0.5439849763322329 1102 34-35 0.44673688977929515 1102 36-37 0.4772531385224781 1102 38-39 0.5020352537861683 1102 40-41 0.6130221119469113 1102 42-43 0.5736397556474131 1102 44-45 0.5610439816614985 1102 46-47 0.6665209369782374 1102 48-49 0.7278033554897902 1102 50-51 0.526846346928096 1102 52-53 0.6486751774435575 1102 54-55 0.570482742649439 1102 56-57 0.6190018005164362 1102 58-59 0.5727448259050476 1102 60-61 0.5283077834390184 1102 62-63 0.6423765607836387 1102 64-65 0.6498183759006935 1102 66-67 0.5576826869176941 1102 68-69 0.6770025905499963 1102 70-71 0.6855567836011396 1102 72-73 0.8815330569623185 1102 74-75 0.9069054209392142 1102 76-77 0.7372007323391969 1102 78-79 0.7885581264777812 1102 80-81 0.977498980543988 1102 82-83 1.0127259581575387 1102 84-85 0.8241161794476106 1102 86-87 0.8671073541778576 1102 88-89 0.7020953233677787 1102 90-91 1.0044520510798236 1102 92-93 0.748437738477211 1102 94-95 0.5130123418677393 1102 96-97 0.1988166936448863 1102 98-99 0.22140522171232746 1102 100-101 0.19027543529730195 1103 1 0.07892086275224841 1103 2 0.17642884416797244 1103 3 0.17327578552247047 1103 4 0.14968867019525334 1103 5 0.185797821806446 1103 6 0.06609347418120848 1103 7 0.10127352864238048 1103 8 0.15038334039065404 1103 9 0.12851716304907512 1103 10-11 0.11491195482475547 1103 12-13 0.30057000370618425 1103 14-15 0.34074456906303396 1103 16-17 0.15492066800907622 1103 18-19 0.08564024596750386 1103 20-21 0.2915733806406706 1103 22-23 0.31702113856037784 1103 24-25 0.29935162728081366 1103 26-27 0.2206984575235751 1103 28-29 0.2771612520888098 1103 30-31 0.4353363584869143 1103 32-33 0.44084179584028504 1103 34-35 0.4020975376900324 1103 36-37 0.5995153443190162 1103 38-39 0.5220265006253015 1103 40-41 0.5484984960957249 1103 42-43 0.5401284119045968 1103 44-45 0.7617274355349934 1103 46-47 0.6708051229342757 1103 48-49 0.5706434467348629 1103 50-51 0.5979096349336146 1103 52-53 0.6558280129371781 1103 54-55 0.3989632292898193 1103 56-57 0.4911702856007025 1103 58-59 0.39197083428685175 1103 60-61 0.6242954759643524 1103 62-63 0.6021274935699736 1103 64-65 0.9008192423752064 1103 66-67 0.806759626317529 1103 68-69 0.6570281427064515 1103 70-71 0.654665199013774 1103 72-73 0.7044031211521649 1103 74-75 0.5759090636687851 1103 76-77 0.7662408907802529 1103 78-79 0.8100617249994713 1103 80-81 0.9016913025188416 1103 82-83 0.8733681218681681 1103 84-85 1.0534852445392993 1103 86-87 0.9814700533343697 1103 88-89 1.0547279028093435 1103 90-91 1.201019751032078 1103 92-93 0.9404352274694503 1103 94-95 0.9756248509303553 1103 96-97 0.6478949592812135 1103 98-99 0.4901232815167482 1103 100-101 0.03286088913542784 1104 1 0.06326185016225594 1104 2 0.0544918964727259 1104 3 0.10107441393012806 1104 4 0.051264977814071244 1104 5 0.026599282108747957 1104 6 0.05989230730413908 1104 7 0.04998041048754942 1104 8 0.014418278464283674 1104 9 0.23629122562370952 1104 10-11 0.11901564311163071 1104 12-13 0.1578953986360716 1104 14-15 0.023026461996941805 1104 16-17 0.11370551225487446 1104 18-19 0.04945085021876139 1104 20-21 0.11492201075428454 1104 22-23 0.09193498754874696 1104 24-25 0.20288296577752618 1104 26-27 0.20702774204368524 1104 28-29 0.10316606289777752 1104 30-31 0.13939142689593353 1104 32-33 -0.04153051300681909 1104 34-35 0.20957476311566126 1104 36-37 0.3217610708876464 1104 38-39 0.20173324599362275 1104 40-41 0.32497074011633487 1104 42-43 0.2449596923986448 1104 44-45 0.3960667169843788 1104 46-47 0.18594637647414203 1104 48-49 0.22160219660691638 1104 50-51 0.27261535672555226 1104 52-53 0.2658027647199077 1104 54-55 0.49325679099441544 1104 56-57 0.206906066418874 1104 58-59 0.5130957447239268 1104 60-61 0.4695204921709788 1104 62-63 0.5826240987083295 1104 64-65 0.3020560638293688 1104 66-67 0.418979422284643 1104 68-69 0.41508624772539093 1104 70-71 0.24045966487750192 1104 72-73 0.17920181779824773 1104 74-75 0.12233934105798383 1104 76-77 0.37495323169942196 1104 78-79 0.4907076016926375 1104 80-81 0.5894066800479258 1104 82-83 0.6511983105474144 1104 84-85 0.5820447313863433 1104 86-87 0.5990559484519089 1104 88-89 0.6393426968998988 1104 90-91 0.6946343404073545 1104 92-93 0.4530132751681073 1104 94-95 0.5226479496372356 1104 96-97 0.6308890907766589 1104 98-99 0.6337916086517588 1104 100-101 0.0693786823047624 1105 1 0.08521976861587177 1105 2 0.15350787265617072 1105 3 0.17666828577652893 1105 4 0.13079364631394697 1105 5 0.1505161579061749 1105 6 0.17661649169416194 1105 7 0.20490653565433803 1105 8 0.06900059590368102 1105 9 0.22454202670544987 1105 10-11 0.2612137399946377 1105 12-13 0.2634320341252092 1105 14-15 0.3327163857359068 1105 16-17 0.17524433590651256 1105 18-19 0.3067514888115852 1105 20-21 0.23345289014238801 1105 22-23 0.18822468857562313 1105 24-25 0.19359301476768564 1105 26-27 0.3655843014990481 1105 28-29 0.2923495925921813 1105 30-31 0.3843252138779931 1105 32-33 0.3572182736018803 1105 34-35 0.6051021784542243 1105 36-37 0.5899611286759168 1105 38-39 0.6806754285071506 1105 40-41 0.6891399039071118 1105 42-43 0.534788145653458 1105 44-45 0.347713682613346 1105 46-47 0.5115532344481224 1105 48-49 0.5743289587432656 1105 50-51 0.4485092081188 1105 52-53 0.5431539842796127 1105 54-55 0.5450405979426449 1105 56-57 0.842208529562626 1105 58-59 0.6440015920525113 1105 60-61 0.4056985449660786 1105 62-63 0.5639216401097258 1105 64-65 0.6818954254313354 1105 66-67 0.6788659235296848 1105 68-69 0.49633151996332714 1105 70-71 0.32758278310645395 1105 72-73 0.29184511159964543 1105 74-75 0.3662845590788315 1105 76-77 0.5270467577901741 1105 78-79 0.5221039999901542 1105 80-81 0.5562677057572145 1105 82-83 0.6588284721273041 1105 84-85 0.5935228448915915 1105 86-87 0.9046747211979245 1105 88-89 1.0739015419137097 1105 90-91 0.9386014725184779 1105 92-93 0.7205697080087248 1105 94-95 0.8417049295184782 1105 96-97 0.605769861892913 1105 98-99 0.7480744010587692 1105 100-101 0.6635881680216471 1106 1 0.0661804978738445 1106 2 0.05547789420327831 1106 3 0.11870905809747967 1106 4 0.07112814997257999 1106 5 0.07118872776240437 1106 6 0.06493167639149533 1106 7 0.14117615110325943 1106 8 0.044836269106355076 1106 9 0.11609865310605727 1106 10-11 0.21035302745559648 1106 12-13 0.15293629432805744 1106 14-15 0.1558339823346202 1106 16-17 0.09024301557956704 1106 18-19 0.15641507888209816 1106 20-21 0.12905018573780325 1106 22-23 0.24782807486266023 1106 24-25 0.16806929810203286 1106 26-27 0.2020969664813208 1106 28-29 0.14573236396623912 1106 30-31 0.12288993255460667 1106 32-33 0.09933810928758646 1106 34-35 0.2047938938323668 1106 36-37 0.15703295602403244 1106 38-39 0.16178420625940504 1106 40-41 0.497069443761454 1106 42-43 0.3184869517341511 1106 44-45 0.34453200718876786 1106 46-47 0.4059985643189137 1106 48-49 0.3844413349723794 1106 50-51 0.2714912563599512 1106 52-53 0.30449707709468754 1106 54-55 0.2695348502235291 1106 56-57 0.36587675718386237 1106 58-59 0.39822976329813287 1106 60-61 0.5741550749593785 1106 62-63 0.47955782675551717 1106 64-65 0.42782467778253164 1106 66-67 0.48578568671459266 1106 68-69 0.5739538533325899 1106 70-71 0.4870731994078845 1106 72-73 0.4349501924364674 1106 74-75 0.6819524648215527 1106 76-77 0.70802373722589 1106 78-79 0.6660176834210674 1106 80-81 0.7464537702922946 1106 82-83 0.6387962217745695 1106 84-85 0.7740404443028339 1106 86-87 0.8433717069462077 1106 88-89 1.053478662288466 1106 90-91 1.0012611422336164 1106 92-93 1.0176878014878739 1106 94-95 1.0280839211637023 1106 96-97 1.1359194104036021 1106 98-99 1.1060908168633183 1106 100-101 0.8221879495817568 1107 1 0.06850995418419359 1107 2 0.16147589415759 1107 3 0.09947280435844874 1107 4 0.10901479292463279 1107 5 0.11754498203203667 1107 6 0.11401017413167835 1107 7 0.049247743011399336 1107 8 0.06282562626926591 1107 9 0.026760576849341078 1107 10-11 0.03144695075081927 1107 12-13 0.032639883724712604 1107 14-15 0.09463877448536806 1107 16-17 0.04999559651546548 1107 18-19 0.0438907691889483 1107 20-21 0.04922080127876427 1107 22-23 0.11961328145175543 1107 24-25 0.042089633128107096 1107 26-27 0.06169545399281873 1107 28-29 0.04720542635750036 1107 30-31 0.02632177219132359 1107 32-33 0.08775199346622031 1107 34-35 0.2247965615425329 1107 36-37 -0.12461754594027497 1107 38-39 -0.1714547992005535 1107 40-41 -0.15176151700477902 1107 42-43 -0.0484709522415514 1107 44-45 -0.08756961046056233 1107 46-47 0.015111594903991943 1107 48-49 -0.38605858064838827 1107 50-51 -0.12070266995671375 1107 52-53 0.05101597325222684 1107 54-55 0.11280982555457797 1107 56-57 0.14218557485029493 1107 58-59 0.0734983213860545 1107 60-61 0.09469791713189579 1107 62-63 0.157762708370349 1107 64-65 -0.09331029287633896 1107 66-67 0.03863619398484275 1107 68-69 0.0747541663832223 1107 70-71 0.00343982237421514 1107 72-73 0.0435484728688138 1107 74-75 -0.22057736452606136 1107 76-77 -0.2165567063951528 1107 78-79 -0.06574147983173972 1107 80-81 0.0879501493547501 1107 82-83 0.03371312849830588 1107 84-85 -0.06295988510778727 1107 86-87 0.1918214504554534 1107 88-89 0.5811178345908523 1107 90-91 0.40033437087774715 1107 92-93 0.45304925779232974 1107 94-95 0.8160127537309876 1107 96-97 0.8898633103747855 1107 98-99 0.6959558027251909 1107 100-101 0.3507295006415063 1108 1 0.11050232109199243 1108 2 0.08349431248032602 1108 3 0.09550638664900646 1108 4 0.14989797180502507 1108 5 0.06546632499214411 1108 6 0.036614265833769366 1108 7 0.12242376410669209 1108 8 0.09033224617414959 1108 9 0.1887144605959321 1108 10-11 0.12768627844197766 1108 12-13 -0.01337933299238614 1108 14-15 0.10574964770503215 1108 16-17 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-0.9449553682713834 2316 94-95 -0.9123311884843623 2316 96-97 -0.9307468988412921 2316 98-99 -0.9512172967896575 2316 100-101 -0.36183065064734876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 20.0 5 44.0 6 168.0 7 316.0 8 575.0 9 1084.0 10 1671.0 11 2521.0 12 4044.0 13 5364.0 14 6497.0 15 8789.0 16 11600.0 17 16847.0 18 15725.0 19 9214.0 20 8204.0 21 8749.0 22 9387.0 23 10231.0 24 11550.0 25 12951.0 26 15074.0 27 17667.0 28 21468.0 29 25233.0 30 30431.0 31 36528.0 32 45316.0 33 58098.0 34 80716.0 35 123933.0 36 171975.0 37 248145.0 38 164711.0 39 9579.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.441407119044484 19.15840499419465 14.332955824783422 19.067232061977446 2 13.837781721978502 23.80362345534309 39.73996182311376 22.61863299956465 3 22.088426375540003 30.325926794146213 29.52957034258732 18.056076487726465 4 11.7408995010214 17.18252905127089 38.60294363886005 32.47362780884766 5 11.52590335219852 41.710592411506646 32.74111717624996 14.022387060044874 6 26.415642476809214 37.53608385519574 18.76460935668598 17.283664311309067 7 25.35757342352902 37.08524496835337 20.59910920598774 16.95807240212987 8 24.513663306654166 32.24063159304779 20.907119654398716 22.338585445899334 9 27.475637118649743 19.64142192156994 21.59756873513948 31.285372224640835 10-11 23.240305080204948 27.400162419209 26.48801949030508 22.87151301028097 12-13 29.672859247848365 25.225042697833295 26.233506915374573 18.86859113894377 14-15 25.914843579612125 25.525995551836427 28.960115134849346 19.599045733702102 16-17 19.03955781381731 29.65940210761795 30.93423719618328 20.366802882381453 18-19 18.88793074578882 29.949599812464417 33.184211848230134 17.978257593516627 20-21 20.415014741223814 28.053869618344002 31.01504931811113 20.516066322321056 22-23 19.46150497304176 27.621939988613914 29.96709755199089 22.949457486353438 24-25 20.615058102541777 28.146704731924583 31.294539700612837 19.943697464920803 26-27 22.090026326155492 25.59066407298151 32.965210879245035 19.354098721617962 28-29 19.593533371286963 26.305800207628682 34.66984528314524 19.430821137939116 30-31 19.284225243628814 29.609775292187134 32.63181909514082 18.474180369043232 32-33 18.659036870834868 29.162327115635776 31.792011319111886 20.386624694417467 34-35 19.846068256245367 27.129611683608562 31.912789872620774 21.1115301875253 36-37 21.676944865289382 26.92374512969955 32.47531201948497 18.923997985526096 38-39 22.690516168490376 25.48073038785372 33.29117850581021 18.5375749378457 40-41 19.488805453121383 27.328902372198222 34.41414234863825 18.768149826042148 42-43 18.74707760418171 29.381479765195305 32.302640694652176 19.568801935970807 44-45 19.517030600955188 29.477735493315393 30.217925216642172 20.787308689087247 46-47 19.577927555141972 28.80836576486293 29.003017733422244 22.61068894657285 48-49 20.727699402772274 28.28080712604406 29.759622851129258 21.231870620054412 50-51 21.834894875334783 29.077918587721935 29.831577836959443 19.255608699983842 52-53 19.470487597310367 29.57184484592551 30.94537726304033 20.012290293723787 54-55 17.897006128394896 29.059977897592177 31.518159137336326 21.5248568366766 56-57 20.300679488424585 30.679957268248902 28.126559323793483 20.89280391953303 58-59 19.70659978843102 28.7114678843395 29.58248406430123 21.99944826292825 60-61 19.82399121253754 27.887090819127803 30.627940212436233 21.660977755898426 62-63 22.507539211434484 27.34385334496518 29.895589665963957 20.25301777763638 64-65 21.113292050371744 28.16279099946042 31.241347929300726 19.48256902086711 66-67 19.382477649465084 28.577456528591565 31.165501331383176 20.874564490560175 68-69 19.019843087152687 30.27914780466854 30.27370583738899 20.42730327078978 70-71 19.344504537691304 29.96446033287566 28.433240681825794 22.257794447607246 72-73 19.42939787682931 28.537264324704463 28.092537088510095 23.940800709956132 74-75 19.948821162068555 29.391664203864742 28.843666357176883 21.81584827688982 76-77 20.87459213328717 27.379769811043612 30.49111873511483 21.254519320554387 78-79 19.48701333222821 27.38014955169237 31.790220744258903 21.34261637182052 80-81 18.804617736542205 30.42326181852888 29.970957052605495 20.801163392323417 82-83 18.629483272495897 29.56284953618432 28.93067043970396 22.87699675161582 84-85 19.119922306687652 27.878168849000367 28.79734436221158 24.2045644821004 86-87 20.285509667778417 27.77517266060128 29.709775978081815 22.229541693538486 88-89 21.27711423492344 25.998994146808347 30.516762329159363 22.207129289108845 90-91 19.266298427884287 27.026848913941848 31.42472645488809 22.282126203285774 92-93 18.199197950504 29.011042831787282 30.447029570342586 22.34272964736613 94-95 17.95519239141355 29.25509025149861 29.380337229161785 23.409380127926056 96-97 18.191997923713206 27.83513613073909 28.340854291550855 25.63201165399685 98-99 19.389487194192082 27.299605630595465 28.510007003290667 24.800900171921786 100-101 20.263957767426984 27.086815632682775 29.468321174849834 23.180905425040407 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4267.0 1 2945.0 2 3024.5 3 4000.5 4 2842.0 5 2474.0 6 3996.0 7 7593.5 8 10660.0 9 10307.5 10 7643.0 11 5405.0 12 4797.5 13 4669.5 14 4433.0 15 4126.5 16 3811.0 17 3532.0 18 3434.0 19 3486.0 20 3573.0 21 3816.5 22 4165.5 23 4583.0 24 5008.5 25 5593.5 26 6598.5 27 7586.0 28 8161.5 29 9295.5 30 11208.5 31 13105.5 32 15839.0 33 19220.0 34 22154.0 35 24560.0 36 29336.5 37 34479.5 38 35235.5 39 34462.5 40 33157.0 41 31530.5 42 29787.0 43 29184.0 44 31447.0 45 33172.0 46 36154.5 47 48922.5 48 111119.5 49 113078.5 50 62783.5 51 48792.0 52 28766.0 53 21244.5 54 18969.0 55 17405.0 56 15916.5 57 14493.5 58 13208.0 59 11902.0 60 10566.0 61 9234.0 62 7559.0 63 5455.5 64 3831.0 65 3030.0 66 2418.5 67 1820.0 68 1300.0 69 938.0 70 731.5 71 590.0 72 507.0 73 550.0 74 489.0 75 304.0 76 225.0 77 173.0 78 119.0 79 90.0 80 63.0 81 40.5 82 31.5 83 18.0 84 11.0 85 7.5 86 3.5 87 2.0 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27602893406115 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012139580054251365 16-17 9.209336592880346E-4 18-19 0.0 20-21 4.186062087672884E-5 22-23 0.0 24-25 0.0 26-27 4.186062087672884E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0012139580054251365 36-37 0.0069907236864137174 38-39 0.0028046615987408325 40-41 0.0034744315327684943 42-43 4.186062087672884E-5 44-45 0.0013814004889320518 46-47 4.186062087672884E-5 48-49 3.7674558789055964E-4 50-51 8.372124175345769E-5 52-53 1.2558186263018654E-4 54-55 0.0 56-57 0.0010046549010414923 58-59 0.0025534978734804595 60-61 9.209336592880346E-4 62-63 0.0015069823515622385 64-65 4.186062087672884E-5 66-67 4.604668296440173E-4 68-69 0.0015907035933156962 70-71 0.0 72-73 0.0 74-75 8.372124175345769E-5 76-77 0.2534660594085931 78-79 2.930243461371019E-4 80-81 1.6744248350691537E-4 82-83 0.0 84-85 0.0 86-87 0.10678644385653528 88-89 0.36883393054485786 90-91 0.27414520612169724 92-93 0.0 94-95 0.0 96-97 0.0 98-99 1.2558186263018654E-4 100-101 5.44188071397475E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1194440.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.946794089777413 #Duplication Level Percentage of deduplicated Percentage of total 1 69.47166443901146 20.804536300291883 2 14.05931236823914 8.420626650710368 3 5.71990622244169 5.138785615688935 4 3.101724815558715 3.715468574987587 5 1.7424839835821933 2.6090904530535513 6 1.196774814130141 2.1503741358352184 7 0.7834267014355053 1.642278267862582 8 0.5597872991073392 1.3411067984352099 9 0.42968349608032047 1.1580878862803732 >10 2.504413143725608 14.28152582552064 >50 0.2483045309049505 5.201787563974709 >100 0.15088543272772134 8.221721708841853 >500 0.014556665122722601 2.99874795799096 >1k 0.012597114048509942 8.17418906767562 >5k 0.003079294545191319 6.791299399647918 >10k+ 0.001399679338723327 7.350373793202522 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 25793 2.159421988546934 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21890 1.832657981983189 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15374 1.2871303707176585 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13737 1.1500786979672484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10886 0.9113894377281403 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9523 0.7972773852181776 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9493 0.7947657479655739 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9357 0.7833796590871036 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8417 0.7046816918388533 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7030 0.5885603295268075 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6833 0.5720672449013764 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6621 0.5543183416496433 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6297 0.5271926593215231 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6240 0.5224205485415759 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6185 0.5178158802451358 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5015 0.4198620273935903 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4592 0.3844479421318777 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4197 0.3513780516392619 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4192 0.3509594454304946 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4070 0.3407454539365728 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3971 0.33245705100298045 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3425 0.28674525300559256 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3423 0.28657781052208564 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3315 0.27753591641271225 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3152 0.2638893540068986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3011 0.2520846589196611 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2515 0.21055892300994608 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2465 0.2063728609222732 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2456 0.2056193697464921 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.20402866615317639 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2423 0.20285656876862798 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.19892167040621547 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2304 0.19289374099996653 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2215 0.18544255048390876 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2164 0.18117276715448244 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1962 0.16426107632028397 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1952 0.1634238639027494 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1919 0.1606610629248853 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1906 0.15957268678209036 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1852 0.15505173972740363 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1776 0.14868892535414086 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1731 0.14492146947523527 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1711 0.1432470446401661 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1650 0.1381400488932052 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.1365493452998895 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1627 0.13621446033287565 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1603 0.13420515053079268 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1568 0.13127490706942166 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1465 0.1226516191688155 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1384 0.11587019858678545 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1371 0.11478182244399049 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1353 0.11327484009242825 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1336 0.11185157898261948 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1298 0.10867017179598808 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.186062087672884E-5 0.0 12-13 0.0 0.0 0.0 1.6744248350691537E-4 0.0 14-15 0.0 0.0 0.0 1.6744248350691537E-4 0.0 16-17 0.0 0.0 0.0 4.186062087672885E-4 0.0 18-19 0.0 0.0 0.0 6.279093131509327E-4 0.0 20-21 0.0 0.0 0.0 0.0012139580054251365 0.0 22-23 0.0 0.0 0.0 0.0030976859448779343 0.0 24-25 0.0 0.0 0.0 0.005944208164495496 0.0 26-27 0.0 0.0 0.0 0.009251197213757074 0.0 28-29 0.0 0.0 0.0 0.017539600147349385 0.0 30-31 0.0 0.0 0.0 0.03846991058571381 0.0 32-33 0.0 0.0 0.0 0.07271189846287801 0.0 34-35 0.0 0.0 0.0 0.13286561066273736 0.0 36-37 0.0 0.0 0.0 0.22433106727838986 0.0 38-39 0.0 0.0 0.0 0.3329593784535012 0.0 40-41 0.0 0.0 0.0 0.46653661967114296 0.0 42-43 0.0 0.0 0.0 0.5850021767522856 0.0 44-45 0.0 0.0 0.0 0.7159003382338167 0.0 46-47 0.0 0.0 0.0 0.8858544589933358 0.0 48-49 0.0 0.0 0.0 1.0833947289106192 0.0 50-51 0.0 0.0 0.0 1.2743210207293796 0.0 52-53 0.0 0.0 0.0 1.463363584608687 0.0 54-55 0.0 0.0 0.0 1.653703827735173 0.0 56-57 0.0 0.0 0.0 1.869034861525066 0.0 58-59 0.0 0.0 0.0 2.160677807173236 0.0 60-61 0.0 0.0 0.0 2.5555071832825424 0.0 62-63 0.0 0.0 0.0 2.905127088844982 0.0 64-65 0.0 0.0 0.0 3.2034258732125513 0.0 66-67 0.0 0.0 0.0 3.5399434044405744 0.0 68-69 0.0 0.0 0.0 3.9174424835069153 0.0 70-71 0.0 0.0 0.0 4.381844211513345 0.0 72-73 0.0 0.0 0.0 4.863701818425371 0.0 74-75 0.0 0.0 0.0 5.336810555574161 0.0 76-77 0.0 0.0 0.0 5.779486621345567 0.0 78-79 0.0 0.0 0.0 6.2935769063326745 0.0 80-81 0.0 0.0 0.0 6.875606979002713 0.0 82-83 0.0 0.0 0.0 7.479990623220923 0.0 84-85 0.0 0.0 0.0 8.243360905528952 0.0 86-87 0.0 0.0 0.0 9.067554669970864 0.0 88-89 0.0 0.0 0.0 9.761687485348782 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 50 5.6097633E-9 66.44198 2 TATCACG 45 9.666662E-4 42.185383 2 TCACGCA 65 0.0058691413 29.205267 4 CTTAACG 60 5.421538E-6 27.68416 20-21 CCGTAAG 70 0.008324747 27.187464 1 CGTAAGA 90 8.993468E-4 26.365864 2 CGTATAG 145 7.7757613E-7 26.249966 1 CATTCCG 165 8.4821295E-8 25.886484 9 TCGACAG 95 0.0012328399 24.978188 5 GTAGATA 175 1.4988473E-7 24.407257 6 TACCTGG 795 0.0 23.87852 2 GTAAGAC 160 1.8738301E-6 23.729277 3 AGTACTT 10390 0.0 22.678703 12-13 CTATACC 105 0.0022074406 22.599314 4 CATATAG 275 5.2750693E-11 22.434954 3 ACTATAC 305 9.094947E-12 21.784256 3 GAGTACT 9915 0.0 21.778763 12-13 TATAGGG 240 5.988113E-9 21.75184 2 GGATATG 525 0.0 21.74997 1 GCGGGAC 110 0.0028920416 21.572071 3 >>END_MODULE