##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939266_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1218565 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01932190732542 33.0 31.0 34.0 30.0 34.0 2 32.01767899127252 33.0 31.0 34.0 30.0 34.0 3 32.06005998859314 34.0 31.0 34.0 30.0 34.0 4 35.55456541095469 37.0 35.0 37.0 33.0 37.0 5 35.63086663411472 37.0 35.0 37.0 33.0 37.0 6 35.77473996052734 37.0 35.0 37.0 35.0 37.0 7 35.72301272398272 37.0 35.0 37.0 35.0 37.0 8 35.73199131765642 37.0 35.0 37.0 35.0 37.0 9 37.3340486555908 39.0 37.0 39.0 34.0 39.0 10-11 37.26103777804221 39.0 37.0 39.0 34.0 39.0 12-13 37.17168349657179 39.0 37.0 39.0 33.5 39.0 14-15 38.249372827875405 40.0 38.0 41.0 33.0 41.0 16-17 38.23466618522606 40.0 38.0 41.0 33.5 41.0 18-19 38.335047371293285 40.0 38.0 41.0 34.0 41.0 20-21 38.26569079203818 40.0 38.0 41.0 34.0 41.0 22-23 38.20010545190449 40.0 38.0 41.0 34.0 41.0 24-25 38.20043042431056 40.0 38.0 41.0 34.0 41.0 26-27 38.002932958028495 40.0 37.5 41.0 33.5 41.0 28-29 37.83007636030905 40.0 37.0 41.0 33.0 41.0 30-31 37.629313577855925 40.0 37.0 41.0 33.0 41.0 32-33 37.34899656563253 40.0 36.0 41.0 33.0 41.0 34-35 37.0506862580166 40.0 35.0 41.0 31.5 41.0 36-37 36.73822980308806 39.0 35.0 41.0 31.0 41.0 38-39 36.32126722825619 39.0 35.0 40.5 30.5 41.0 40-41 36.144614772293636 39.0 35.0 40.0 30.0 41.0 42-43 36.00209139438602 39.0 35.0 40.5 30.0 41.0 44-45 35.84238592114495 39.0 35.0 41.0 29.5 41.0 46-47 35.45840107011116 39.0 35.0 41.0 26.5 41.0 48-49 35.170417663399164 39.0 35.0 41.0 24.5 41.0 50-51 33.97549740883744 38.0 34.0 40.0 23.0 40.5 52-53 34.0290452294297 38.0 33.5 39.5 23.0 40.5 54-55 34.553347585069325 38.0 35.0 40.0 23.0 41.0 56-57 34.537075986919035 38.5 35.0 41.0 23.0 41.0 58-59 34.38414323404989 38.0 35.0 40.0 22.5 41.0 60-61 34.04479120933229 38.0 34.0 40.0 21.5 41.0 62-63 33.59756311727318 37.5 34.0 40.0 19.5 41.0 64-65 33.12093322883884 37.0 33.0 39.5 17.5 41.0 66-67 32.72678519406023 36.0 33.0 39.0 13.0 41.0 68-69 32.34454953162121 36.0 33.0 39.0 9.0 40.5 70-71 31.842535687468455 35.0 32.0 38.5 6.0 40.0 72-73 31.30696351856487 35.0 31.5 37.0 2.0 39.5 74-75 30.85382560634845 35.0 31.0 37.0 2.0 39.0 76-77 30.32105386253503 35.0 30.5 36.0 2.0 39.0 78-79 29.88670239174767 34.5 30.5 36.0 2.0 37.5 80-81 29.488444604924645 34.0 30.0 35.5 2.0 37.0 82-83 29.062158768715662 34.0 29.0 35.0 2.0 37.0 84-85 28.64982992290112 34.0 29.0 35.0 2.0 36.0 86-87 28.273785559243862 34.0 29.0 35.0 2.0 36.0 88-89 27.752455962546108 34.0 28.0 35.0 2.0 35.5 90-91 27.26774730933516 34.0 26.0 35.0 2.0 35.0 92-93 26.899265939855486 34.0 25.0 35.0 2.0 35.0 94-95 26.46523287637508 34.0 25.0 35.0 2.0 35.0 96-97 25.816944110490617 33.0 22.0 35.0 2.0 35.0 98-99 25.079746669238 33.0 10.5 35.0 2.0 35.0 100-101 22.763953502685535 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12802843400284303 1101 2 -0.07050308604215871 1101 3 0.08581198952305868 1101 4 0.03304409237633621 1101 5 0.10733471550682339 1101 6 0.13776241036848802 1101 7 0.22052235592295233 1101 8 0.19691150025533233 1101 9 0.23675177757176158 1101 10-11 0.2224254343730152 1101 12-13 0.19650646477477807 1101 14-15 0.23400972896425998 1101 16-17 0.2819553094335632 1101 18-19 0.2791629752920386 1101 20-21 0.286572416959892 1101 22-23 0.2566471587222665 1101 24-25 0.2844391058963467 1101 26-27 0.3177562329729682 1101 28-29 0.21935682218252595 1101 30-31 0.3107344315717171 1101 32-33 0.302064048239032 1101 34-35 0.25660437692575044 1101 36-37 0.3448584650568307 1101 38-39 0.4251554950175489 1101 40-41 0.4096037473080898 1101 42-43 0.3598786463990322 1101 44-45 0.34750937225505396 1101 46-47 0.1894846708174498 1101 48-49 0.1329889115802132 1101 50-51 0.2949839144356545 1101 52-53 0.3915465437292198 1101 54-55 0.33837360709289044 1101 56-57 0.3171529586526276 1101 58-59 0.3127625915071519 1101 60-61 0.5122617344659446 1101 62-63 0.5013893506850877 1101 64-65 0.5706584295566799 1101 66-67 0.4322187174960135 1101 68-69 0.4400700532865969 1101 70-71 0.6313391967329061 1101 72-73 0.621068477245025 1101 74-75 0.5719393598985256 1101 76-77 0.4710620764027844 1101 78-79 0.15418095637554785 1101 80-81 0.25486101365827807 1101 82-83 0.28561765578653464 1101 84-85 0.4437680496054135 1101 86-87 0.3087731090191319 1101 88-89 0.3541222889942546 1101 90-91 0.24542339736769847 1101 92-93 0.25948146269335126 1101 94-95 0.09703544870249559 1101 96-97 0.23511416838436006 1101 98-99 0.2640092579828135 1101 100-101 0.1657512658099094 1102 1 0.36647195901649354 1102 2 0.329689084327903 1102 3 0.35250350882410686 1102 4 0.18165059466557665 1102 5 0.3334429329778743 1102 6 0.33826016313027907 1102 7 0.38835289596019607 1102 8 0.36844893322322747 1102 9 0.3580763879804678 1102 10-11 0.38758781658552977 1102 12-13 0.34176486216490076 1102 14-15 0.5533337023054443 1102 16-17 0.5292915270726226 1102 18-19 0.40532947394089547 1102 20-21 0.467938016713461 1102 22-23 0.47291758338727874 1102 24-25 0.4788243357306996 1102 26-27 0.571634644084547 1102 28-29 0.6911665840172745 1102 30-31 0.47661789021833556 1102 32-33 0.6498714158200016 1102 34-35 0.5441948615171199 1102 36-37 0.5612566532647776 1102 38-39 0.5171714987139424 1102 40-41 0.5049818573877545 1102 42-43 0.604413692113873 1102 44-45 0.6952493576935126 1102 46-47 0.6637980730380875 1102 48-49 0.6769159428455325 1102 50-51 0.67830342130339 1102 52-53 0.6806421878674982 1102 54-55 0.6139700703179898 1102 56-57 0.6078587734689975 1102 58-59 0.6593115756994763 1102 60-61 0.6393173273587962 1102 62-63 0.7469386289085804 1102 64-65 0.6468912602367283 1102 66-67 0.6515598426552742 1102 68-69 0.4800451656485123 1102 70-71 0.37045479298611284 1102 72-73 0.53783490448631 1102 74-75 0.2617233520016846 1102 76-77 0.491875282602642 1102 78-79 0.5172350651110769 1102 80-81 0.3810966446351607 1102 82-83 0.07728957300976091 1102 84-85 0.28606058261391 1102 86-87 -0.005880494262818559 1102 88-89 0.12190679915233105 1102 90-91 0.07170085777850588 1102 92-93 0.1769015842262327 1102 94-95 0.2782969124176482 1102 96-97 0.24107179989330163 1102 98-99 0.36944480915123634 1102 100-101 0.3736663048040434 1103 1 0.18741678431503317 1103 2 0.15585145717177085 1103 3 0.16442992412707014 1103 4 0.27976813115385823 1103 5 0.2575086118810148 1103 6 0.16436034004315303 1103 7 0.13467642537196411 1103 8 0.32345363247134884 1103 9 0.204099994791612 1103 10-11 0.29519576107912116 1103 12-13 0.3452409236421232 1103 14-15 0.5186007301692186 1103 16-17 0.4341317526365387 1103 18-19 0.5283011955242713 1103 20-21 0.5720851031796315 1103 22-23 0.562569562600018 1103 24-25 0.6204168279907734 1103 26-27 0.5676852024657961 1103 28-29 0.6045518394352385 1103 30-31 0.47801384333642716 1103 32-33 0.5714630788009814 1103 34-35 0.6748996105219902 1103 36-37 0.584980945613303 1103 38-39 0.7936600546626309 1103 40-41 0.8449206289490121 1103 42-43 0.6991622823394366 1103 44-45 0.8006403627797596 1103 46-47 0.8741930863065477 1103 48-49 0.9144836673037346 1103 50-51 0.8779869131211697 1103 52-53 0.871659713298726 1103 54-55 0.8989172728832244 1103 56-57 0.9320101445437459 1103 58-59 0.9526344969732463 1103 60-61 0.9808628271376563 1103 62-63 0.8391924712349805 1103 64-65 0.8104043628461923 1103 66-67 0.798183294667659 1103 68-69 0.80041295092051 1103 70-71 0.8334654906862404 1103 72-73 0.8683890011249673 1103 74-75 0.8339777471808141 1103 76-77 0.8799744367379816 1103 78-79 0.7439737869155856 1103 80-81 0.9500442894825731 1103 82-83 0.979942575000031 1103 84-85 0.9673168022070122 1103 86-87 0.8387437041007395 1103 88-89 0.7004533493513598 1103 90-91 0.9006413155405575 1103 92-93 0.972083472771125 1103 94-95 0.9806686505866011 1103 96-97 0.853152322891976 1103 98-99 0.6265489666036892 1103 100-101 0.6441425874223512 1104 1 0.323160838254303 1104 2 0.19543987828028975 1104 3 0.2329390290325648 1104 4 0.282323079125824 1104 5 0.27902326467354044 1104 6 0.27880873673034756 1104 7 0.30938177682661916 1104 8 0.3608520981753216 1104 9 0.4220370568005265 1104 10-11 0.3119638514380014 1104 12-13 0.40969529293496265 1104 14-15 0.4042386044525088 1104 16-17 0.5989557598567572 1104 18-19 0.4304085298839979 1104 20-21 0.5859332529991264 1104 22-23 0.501340400357499 1104 24-25 0.5135835035057355 1104 26-27 0.40229545676606904 1104 28-29 0.4076068272245763 1104 30-31 0.512449233015758 1104 32-33 0.7077247585631383 1104 34-35 0.536489451783396 1104 36-37 0.5959937668067568 1104 38-39 0.7285155176059348 1104 40-41 0.8488277084775788 1104 42-43 0.5979382299928702 1104 44-45 0.6972528999426402 1104 46-47 0.8516000463829982 1104 48-49 0.744712351737796 1104 50-51 0.7309977699909851 1104 52-53 0.7215714514596883 1104 54-55 0.5115705697615454 1104 56-57 0.41313007446517247 1104 58-59 0.4636871355377039 1104 60-61 0.3691784196201269 1104 62-63 0.19362845817489216 1104 64-65 0.3130953535023835 1104 66-67 0.38836632184965225 1104 68-69 0.4080177369685032 1104 70-71 0.2798729683885135 1104 72-73 0.20383542021606615 1104 74-75 0.2707096037320973 1104 76-77 0.22025534829032978 1104 78-79 0.2991407544246094 1104 80-81 0.1494729102088428 1104 82-83 0.22446934554579556 1104 84-85 0.37492556999210436 1104 86-87 0.3748298556191081 1104 88-89 0.17899842261532228 1104 90-91 0.2725521511744482 1104 92-93 0.311413744591043 1104 94-95 0.23798177439208246 1104 96-97 -0.057081073264324544 1104 98-99 -0.09783519139163488 1104 100-101 -0.19025364850883975 1105 1 0.1778539790485283 1105 2 0.10247958947162772 1105 3 0.1598343358917731 1105 4 0.13340611161680016 1105 5 0.22757922135224362 1105 6 0.14794231073756947 1105 7 0.20468863603240095 1105 8 0.2245705097276698 1105 9 0.3958637716019737 1105 10-11 0.4098880391636328 1105 12-13 0.2481429463645739 1105 14-15 0.19349853225033797 1105 16-17 0.284425073936184 1105 18-19 0.280137771761261 1105 20-21 0.3408971519160957 1105 22-23 0.4002573678665229 1105 24-25 0.30239216776566735 1105 26-27 0.3783784892570452 1105 28-29 0.4820653109790882 1105 30-31 0.3206622294056558 1105 32-33 0.3706308957472544 1105 34-35 0.3875724842743722 1105 36-37 0.4227085682508047 1105 38-39 0.7154288750066584 1105 40-41 0.6985569503548348 1105 42-43 0.5343100711481057 1105 44-45 0.6584442356827083 1105 46-47 0.6756072665996058 1105 48-49 0.6765715575139666 1105 50-51 0.5938136544675245 1105 52-53 0.5297844478495861 1105 54-55 0.5332312195810402 1105 56-57 0.5345199661619233 1105 58-59 0.6410337059348166 1105 60-61 0.6602434015695877 1105 62-63 0.5726898300160457 1105 64-65 0.5698643065709703 1105 66-67 0.5684204734742053 1105 68-69 0.37281918101182754 1105 70-71 0.06326647019356813 1105 72-73 0.06213866931354062 1105 74-75 0.3379478708801109 1105 76-77 0.3068979475683058 1105 78-79 0.06493497825132621 1105 80-81 0.01183753114747077 1105 82-83 0.046671511610774985 1105 84-85 0.08882448695961287 1105 86-87 0.11445079424726501 1105 88-89 0.16049661756116862 1105 90-91 0.05143301760044139 1105 92-93 0.4003126615585515 1105 94-95 0.1856352039949698 1105 96-97 0.1965290350389246 1105 98-99 0.0849265414603515 1105 100-101 -0.05983848605396602 1106 1 0.15693807185103026 1106 2 0.24450453203808564 1106 3 0.16158258764002653 1106 4 0.30767588516454936 1106 5 0.2183014954680189 1106 6 0.2284029151151401 1106 7 0.2414997491070494 1106 8 0.330992968135682 1106 9 0.33672931400181483 1106 10-11 0.38096614877965607 1106 12-13 0.48069829715097256 1106 14-15 0.5554040004283678 1106 16-17 0.4264831512379388 1106 18-19 0.4293482183543773 1106 20-21 0.35710477101179805 1106 22-23 0.3110334149241609 1106 24-25 0.49179820396773266 1106 26-27 0.5263345494423461 1106 28-29 0.4990429182093976 1106 30-31 0.2674652008024907 1106 32-33 0.304713284477657 1106 34-35 0.4484977954253182 1106 36-37 0.3009478830875878 1106 38-39 0.5157715828403369 1106 40-41 0.6828763313348603 1106 42-43 0.5554232089128632 1106 44-45 0.5765588376872088 1106 46-47 0.32481140181457135 1106 48-49 0.4505863513843593 1106 50-51 0.36188628580730864 1106 52-53 0.40318609133245786 1106 54-55 0.3382490807812104 1106 56-57 0.3969808356173772 1106 58-59 0.41189123288440044 1106 60-61 0.3209942031146156 1106 62-63 0.4342118819713079 1106 64-65 0.3304642661781543 1106 66-67 0.31407824530525374 1106 68-69 0.27815166441290273 1106 70-71 0.49809360154597115 1106 72-73 0.4243234159748255 1106 74-75 0.151912110611498 1106 76-77 0.2992816919827348 1106 78-79 0.2813426680344655 1106 80-81 0.18999826932626007 1106 82-83 0.05452286603252787 1106 84-85 0.18468169834028814 1106 86-87 0.08503188283377128 1106 88-89 0.15004970887418878 1106 90-91 0.07355755371414574 1106 92-93 0.25690710502122727 1106 94-95 0.30592060451502334 1106 96-97 0.39038301499399 1106 98-99 0.3555442360401351 1106 100-101 0.41089880914181265 1107 1 0.11909130323503092 1107 2 0.06316663916172871 1107 3 0.1329957898224592 1107 4 0.13390734601139798 1107 5 0.3046190240932418 1107 6 0.14309399326775463 1107 7 0.16107056290863397 1107 8 0.19799135436483795 1107 9 0.2144573551913922 1107 10-11 0.2870600548735567 1107 12-13 0.3683587136575355 1107 14-15 0.4154622114481228 1107 16-17 0.3671146351386554 1107 18-19 0.29397397721090357 1107 20-21 0.3363562887248577 1107 22-23 0.3405711848465458 1107 24-25 0.18253535143565358 1107 26-27 0.283073003296181 1107 28-29 0.24686240641350565 1107 30-31 0.3834309658067099 1107 32-33 0.39755793982231324 1107 34-35 0.5198255061761046 1107 36-37 0.29109715689071436 1107 38-39 0.5708319071314278 1107 40-41 0.4060473910443889 1107 42-43 0.5423641525845753 1107 44-45 0.7670366291265296 1107 46-47 0.7700872797827572 1107 48-49 0.7422466141984714 1107 50-51 0.6554773101675622 1107 52-53 0.7469432956665827 1107 54-55 0.783760300330897 1107 56-57 0.7191058260115994 1107 58-59 0.5965863454641323 1107 60-61 0.4155246726450841 1107 62-63 0.4732977960572242 1107 64-65 0.6353516864501927 1107 66-67 0.32280989826767836 1107 68-69 0.5364222399911753 1107 70-71 0.61691228286465 1107 72-73 0.4686252679689815 1107 74-75 0.5493368013054187 1107 76-77 0.31152425710991594 1107 78-79 0.28853739638304177 1107 80-81 0.45720221922251625 1107 82-83 0.49416015182366024 1107 84-85 0.3266743211021428 1107 86-87 0.297257157559045 1107 88-89 0.4061445755459765 1107 90-91 0.48030465276893963 1107 92-93 0.5910777420764788 1107 94-95 0.4243406460119914 1107 96-97 0.4996583550385587 1107 98-99 0.9161230802728291 1107 100-101 0.7165835090572799 1108 1 0.19066335746232 1108 2 0.2835009505399526 1108 3 0.3549307315330168 1108 4 0.27881434435833796 1108 5 0.23709849896745538 1108 6 0.12433211165204483 1108 7 0.19126572077159665 1108 8 0.09780139022490886 1108 9 0.2983916039331689 1108 10-11 0.22639021339902143 1108 12-13 0.25832228674058655 1108 14-15 0.3479222010039891 1108 16-17 0.32728451877233056 1108 18-19 0.1641422382489779 1108 20-21 0.27435223436152256 1108 22-23 0.3598914094024437 1108 24-25 0.29040890801640984 1108 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2316 72-73 -1.7453144017478124 2316 74-75 -1.6382456625319115 2316 76-77 -1.3818351562852769 2316 78-79 -1.5106859258067118 2316 80-81 -1.3950095750865117 2316 82-83 -1.1448543641244555 2316 84-85 -1.1533120452622079 2316 86-87 -1.0549699586899628 2316 88-89 -1.3873971978442619 2316 90-91 -1.2863670633924151 2316 92-93 -1.3822684424135545 2316 94-95 -1.4369955081001322 2316 96-97 -1.5093113398987263 2316 98-99 -1.610968133259508 2316 100-101 -1.4313746834394792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3566.0 3 841.0 4 741.0 5 798.0 6 887.0 7 1151.0 8 1457.0 9 1832.0 10 2410.0 11 3532.0 12 5240.0 13 8237.0 14 7608.0 15 9100.0 16 12150.0 17 16874.0 18 13543.0 19 8503.0 20 8218.0 21 8519.0 22 9470.0 23 10257.0 24 11437.0 25 12620.0 26 14533.0 27 16860.0 28 19832.0 29 23355.0 30 27619.0 31 33927.0 32 41834.0 33 54011.0 34 73540.0 35 118403.0 36 160207.0 37 236141.0 38 206331.0 39 32978.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.407239714743255 18.603818674672027 13.760691778871712 19.228249831713004 2 13.391137038981354 23.21546668112581 40.536368438650925 22.85702784124191 3 22.061630357531666 30.560227426782628 29.168224337459275 18.209917878226435 4 11.429104321254941 16.539462992070344 39.132281688011524 32.89915099866319 5 11.621392657500389 41.50191002935915 32.741083174403876 14.13561413873658 6 26.206290074530724 37.167569733481024 19.42667915824703 17.199461033741226 7 25.603043568589097 37.07316832829548 20.73977176520245 16.584016337912974 8 24.45373320632001 31.718293203692145 21.22507637223664 22.60289721775121 9 27.72086079500196 19.65940687722848 21.838164889904235 30.78156743786532 10-11 23.201857101096063 27.15764009551556 26.712251152066163 22.928251651322206 12-13 29.662995439159506 25.12752944345264 26.276197733868305 18.93327738351955 14-15 25.999568899153196 25.36609699769053 29.008611752630227 19.625722350526047 16-17 19.2158305400403 29.472211319118607 30.905577115416516 20.406381025424576 18-19 18.971879384415267 30.02373666224179 33.05961511377872 17.944768839564222 20-21 20.530899104779625 28.066731324665 30.802405211839805 20.599964358715567 22-23 19.472010486366 27.772854536987683 29.770052259356873 22.985082717289444 24-25 20.605930929574317 28.151709840474027 31.12547043816664 20.116888791785012 26-27 22.15215969984 25.60132207076233 32.66268796961603 19.58383025978165 28-29 19.64781071581542 26.369352362171583 34.47736058027745 19.50547634173555 30-31 19.39487143687235 29.709570320119415 32.389120333773405 18.506437909234823 32-33 18.763506669004435 29.273281155109427 31.437099316695484 20.526112859190654 34-35 19.90509728823542 27.206691640631554 31.593546253658488 21.294664817474537 36-37 21.71886054462389 26.973938339212573 32.27769180148564 19.0295093146779 38-39 22.788333001917273 25.647362607458057 32.88104304250648 18.683261348118194 40-41 19.451058070379958 27.54148472334736 34.22143901523384 18.78601819103884 42-43 18.786378606363563 29.510617807910666 31.967801343628143 19.73520224209763 44-45 19.485870899892692 29.7047107431891 30.03350992821183 20.775908428706373 46-47 19.5616983058204 28.868546965396174 28.902479149619932 22.667275579163498 48-49 20.689023220032272 28.381920151950325 29.634019476459972 21.295037151557434 50-51 21.885557797636206 29.202034345015033 29.78482889264094 19.12757896470782 52-53 19.417572484284175 29.62662949365499 30.93356010517711 20.022237916883725 54-55 17.874494202189005 29.093601263943796 31.548071740422113 21.48383279344508 56-57 20.342060141492443 30.76149445989366 28.20985507007725 20.686590328536642 58-59 19.67693829408634 28.754698093348967 29.605345752092145 21.963017860472544 60-61 19.888318908270424 27.800297223771974 30.75615965820912 21.555224209748477 62-63 22.608112086197348 27.33101535915219 29.93188120279392 20.12899135185654 64-65 21.136427752163698 28.188218529859338 31.332977779242093 19.342375938734875 66-67 19.437788451162948 28.676915752285577 31.242674174462348 20.64262162208913 68-69 19.10673705828975 30.512849134857735 30.360197565565826 20.02021624128669 70-71 19.461763900244875 30.053815371878017 28.513291140544002 21.971129587333103 72-73 19.588681386838168 28.823231919714743 28.16017349891398 23.427913194533105 74-75 20.149007849888388 29.56438473513834 29.056613098523098 21.22999431645017 76-77 21.22980576639258 27.539680186590765 30.49090435905545 20.739609687961206 78-79 19.811676920232976 27.68952835241802 31.766334789541485 20.73245993780752 80-81 19.276536021899147 30.760619904954932 29.956322744820508 20.006521328325412 82-83 19.180226685887245 29.794161111104252 28.895924356236208 22.1296878467723 84-85 19.696295250068253 28.225632773133118 28.847009874913372 23.231062101885254 86-87 20.92320209632246 28.135610388391168 29.8125201244815 21.12866739080487 88-89 22.100420177930097 26.29746357160712 30.513574139804806 21.088542110657972 90-91 20.05889474119967 27.49545767409023 31.460534385952915 20.985113198757187 92-93 19.245644702633776 29.54880206668622 30.345474675515106 20.860078555164904 94-95 19.198936671488497 29.838976739894758 29.24136931142312 21.72071727719363 96-97 19.55995749097919 28.351333761718816 28.20910154383537 23.879607203466627 98-99 20.69732155507303 27.881865389114225 28.41444890723796 23.00636414857478 100-101 21.935755646128975 27.72094291156848 29.19799973481333 21.145301707489217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5297.0 1 3502.5 2 3308.5 3 4314.0 4 2912.5 5 2590.5 6 4326.0 7 8068.5 8 11292.5 9 10791.5 10 7857.5 11 5678.5 12 5054.5 13 4824.0 14 4622.5 15 4421.0 16 4169.0 17 3872.0 18 3719.5 19 3706.0 20 3764.0 21 3944.0 22 4286.0 23 4826.0 24 5250.0 25 5767.0 26 6922.5 27 8034.5 28 8552.0 29 9653.0 30 11569.5 31 13499.5 32 16441.0 33 20119.5 34 23008.0 35 25127.5 36 29902.0 37 34794.5 38 35291.0 39 35132.0 40 33859.0 41 31652.5 42 30068.0 43 29757.5 44 31613.0 45 33377.0 46 36968.0 47 50262.5 48 114957.5 49 116757.5 50 61921.5 51 45596.5 52 26636.5 53 20944.0 54 19439.5 55 17986.5 56 16506.0 57 14776.5 58 13286.0 59 12077.5 60 10685.5 61 9397.0 62 7860.0 63 5742.5 64 4063.5 65 3244.0 66 2582.0 67 2012.0 68 1515.0 69 1035.0 70 819.5 71 693.5 72 583.5 73 573.5 74 470.5 75 322.0 76 255.5 77 199.5 78 148.5 79 105.5 80 70.5 81 46.5 82 35.0 83 29.0 84 22.0 85 11.5 86 6.5 87 5.5 88 2.0 89 4.0 90 5.5 91 3.5 92 3.0 93 3.0 94 1.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.277293373763402 2 0.11012953761186314 3 0.1206336961918322 4 0.12211084349214034 5 0.128675942604621 6 0.05563921497827363 7 0.0643379713023105 8 0.06934385937557701 9 0.06622543729714869 10-11 0.06179399539622425 12-13 0.06622543729714869 14-15 0.061752963526771246 16-17 0.06294288774090837 18-19 0.06798980768362788 20-21 0.07188783528166327 22-23 0.08075071908351217 24-25 0.08887502923520699 26-27 0.08871090175739496 28-29 0.08284334442561538 30-31 0.07980698608609307 32-33 0.07217505836783429 34-35 0.23790277908851803 36-37 0.3615728336198725 38-39 0.33477902286706085 40-41 0.3201306454723384 42-43 0.34191856815188354 44-45 0.35270994981802367 46-47 0.3593160807999573 48-49 0.3679738052545412 50-51 0.36231140727002664 52-53 0.3629268853118217 54-55 0.3504531969981084 56-57 0.3292807523603583 58-59 0.3154119804852429 60-61 0.2723695494290415 62-63 0.33994903841813934 64-65 0.3690816657297723 66-67 0.3724052471554657 68-69 0.38483790359972586 70-71 0.3850840948164439 72-73 0.3687944426436013 74-75 0.37215905593874765 76-77 0.35755171041347816 78-79 0.33724093503424113 80-81 0.33551759651721497 82-83 0.3480733485698342 84-85 0.3550487663768449 86-87 0.34860676287272324 88-89 0.3640757776565058 90-91 0.38606885968331606 92-93 0.3808578122627845 94-95 0.3808578122627845 96-97 0.38610989155276904 98-99 0.3756057329728 100-101 0.3550077345073919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1218565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.974956053846636 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45917270399877 21.824697780967526 2 13.589378049970572 8.418607757938931 3 5.6720608561655 5.270755072634103 4 2.929048283797791 3.62908567480926 5 1.7076130941870962 2.644662027470919 6 1.124205682317065 2.0893332963153455 7 0.7710314066743384 1.6717864756511231 8 0.5570821914582629 1.3804477119040215 9 0.4017235290191487 1.1199031791447884 >10 2.3856756416366887 14.101095672221225 >50 0.23294477532631963 5.042834727667393 >100 0.1400837421551218 7.911932061600263 >500 0.013796126117334738 2.846047105317144 >1k 0.012204265411488422 8.44782583406348 >5k 0.0026531011764105265 6.240479297657309 >10k+ 0.0013265505882052633 7.360506324637194 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 26817 2.2007032862424247 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 21457 1.76084164570622 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15791 1.295868501064777 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 15009 1.2316946572402785 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10492 0.8610127486018391 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9765 0.8013524104171711 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9335 0.7660650026875875 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9070 0.7443181118774953 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8618 0.7072253018919795 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6890 0.5654191610623972 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6696 0.5494987957146316 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6676 0.5478575209365114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6361 0.5220074431811187 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6299 0.5169194913689463 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6227 0.5110109021677136 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4814 0.39505483909352396 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4807 0.39448039292118187 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4288 0.3518893124289636 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4099 0.336379265775728 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4034 0.33104512274683745 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4030 0.33071686779121345 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3696 0.30330757899660665 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3406 0.27950909471386426 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3394 0.27852432984699216 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3079 0.25267425209159955 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3067 0.25168948722472745 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2738 0.2246905171246507 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2634 0.21615588827842586 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2562 0.21024729907719325 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2457 0.20163060649206238 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2426 0.19908663058597612 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.1908802566953753 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2318 0.19022374678412723 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2266 0.1859564323610148 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2133 0.17504195508651568 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2092 0.17167734179136937 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1992 0.1634709679007685 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1977 0.1622400118171784 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1772 0.1454169453414467 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1731 0.14205233204630038 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1722 0.1413137583961463 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1712 0.14049312100708622 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1681 0.13794914510099993 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1655 0.13581548788944373 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1555 0.12760911399884292 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1517 0.12449069192041458 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1508 0.12375211827026052 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1487 0.12202877975323434 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1457 0.11956686758605409 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1382 0.11341208716810346 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1359 0.11152462117326528 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1342 0.11012953761186314 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1280 0.10504158579969063 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1249 0.10249760989360435 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.103186945300415E-5 0.0 12-13 0.0 0.0 0.0 2.0515934726502075E-4 0.0 14-15 0.0 0.0 0.0 3.282549556240332E-4 0.0 16-17 0.0 0.0 0.0 6.565099112480664E-4 0.0 18-19 0.0 0.0 0.0 7.385736501540746E-4 0.0 20-21 0.0 0.0 0.0 0.001066828605778108 0.0 22-23 0.0 0.0 0.0 0.0020926253421032115 0.0 24-25 0.0 0.0 0.0 0.004759696856548481 0.0 26-27 0.0 0.0 0.0 0.007960182673882803 0.0 28-29 0.0 0.0 0.0 0.015263855436517544 0.0 30-31 0.0 0.0 0.0 0.03487708903505352 0.0 32-33 0.0 0.0 0.0 0.06790774394472186 0.0 34-35 0.0 0.0 0.0 0.12317767209791845 0.0 36-37 0.0 0.0 0.0 0.21016523533828724 0.0 38-39 0.0 0.0 0.0 0.3122525265373616 0.0 40-41 0.0 0.0 0.0 0.4380972701497253 0.0 42-43 0.0 0.0 0.0 0.5597157312084295 0.0 44-45 0.0 0.0 0.0 0.6869965902516484 0.0 46-47 0.0 0.0 0.0 0.8562940836147436 0.0 48-49 0.0 0.0 0.0 1.0498003799551112 0.0 50-51 0.0 0.0 0.0 1.2410909553450165 0.0 52-53 0.0 0.0 0.0 1.425447144797364 0.0 54-55 0.0 0.0 0.0 1.622974564344126 0.0 56-57 0.0 0.0 0.0 1.8431926076983993 0.0 58-59 0.0 0.0 0.0 2.141535330491193 0.0 60-61 0.0 0.0 0.0 2.533964129939724 0.0 62-63 0.0 0.0 0.0 2.875103092572001 0.0 64-65 0.0 0.0 0.0 3.1731996241480758 0.0 66-67 0.0 0.0 0.0 3.505270543631238 0.0 68-69 0.0 0.0 0.0 3.8787836512619354 0.0 70-71 0.0 0.0 0.0 4.3225022875267225 0.0 72-73 0.0 0.0 0.0 4.803354765646478 0.0 74-75 0.0 0.0 0.0 5.278750005128984 0.0 76-77 0.0 0.0 0.0 5.7285003262033625 0.0 78-79 0.0 0.0 0.0 6.242506554841145 0.0 80-81 0.0 0.0 0.0 6.821261073475769 0.0 82-83 0.0 0.0 0.0 7.417166913541748 0.0 84-85 0.0 0.0 0.0 8.174245936819126 0.0 86-87 0.0 0.0 0.0 8.99377546540398 0.0 88-89 0.0 0.0 0.0 9.691071054888331 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTATCG 30 1.3168997E-4 63.180542 8 TATCACG 55 5.017683E-5 43.10242 2 GTACGTG 50 0.0016167536 37.995686 1 TATAACG 65 1.3426895E-4 36.471275 2 TACCTGG 840 0.0 32.17288 2 TCACGCA 75 3.1119643E-4 31.60844 4 CGACCGT 60 1.5359547E-7 31.598055 18-19 ATCACGC 80 4.5424886E-4 29.632914 3 GTGTACG 105 6.911492E-5 27.139778 1 GTACGTA 90 9.044508E-4 26.340368 3 GATAGTC 180 6.873961E-9 26.325228 7 GTATAGG 335 0.0 25.519493 1 TCGACCG 75 1.0811764E-6 25.278446 18-19 CATGGGG 2685 0.0 24.721685 4 TAGGACA 470 0.0 24.210722 4 AGTACTC 510 0.0 24.171162 5 TACCGCT 50 0.0016441965 23.7747 94-95 TGTACGT 120 1.7323783E-4 23.70633 2 ATGGGAT 2870 0.0 23.62373 5 GGAACGC 325 0.0 23.328201 8 >>END_MODULE