##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939266_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1218565 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19817654372151 33.0 31.0 34.0 31.0 34.0 2 32.35968947081198 34.0 31.0 34.0 30.0 34.0 3 32.441407721377196 34.0 31.0 34.0 30.0 34.0 4 35.9673017032329 37.0 35.0 37.0 35.0 37.0 5 35.92285516160402 37.0 35.0 37.0 35.0 37.0 6 36.01065433522216 37.0 35.0 37.0 35.0 37.0 7 35.96326909110306 37.0 35.0 37.0 35.0 37.0 8 35.97696799103864 37.0 35.0 37.0 35.0 37.0 9 37.62390352586854 39.0 37.0 39.0 35.0 39.0 10-11 37.54070853832171 39.0 37.0 39.0 35.0 39.0 12-13 37.46076409547295 39.0 37.0 39.0 34.5 39.0 14-15 38.54454050460993 40.0 38.0 41.0 34.0 41.0 16-17 38.51873884446049 40.0 38.0 41.0 34.0 41.0 18-19 38.78956190272985 40.0 38.0 41.0 34.0 41.0 20-21 38.81516373767505 40.0 38.0 41.0 34.5 41.0 22-23 38.76715316786548 40.0 38.0 41.0 34.5 41.0 24-25 38.66630052561825 40.0 38.0 41.0 34.0 41.0 26-27 38.35133784410351 40.0 37.5 41.0 34.0 41.0 28-29 38.14460205241411 40.0 37.5 41.0 33.5 41.0 30-31 37.920046119821265 40.0 37.0 41.0 33.0 41.0 32-33 37.62625260039473 40.0 36.0 41.0 33.0 41.0 34-35 37.538892467779725 40.0 36.0 41.0 33.0 41.0 36-37 37.344261898216345 40.0 35.0 41.0 33.0 41.0 38-39 37.136179440571496 40.0 35.0 41.0 32.5 41.0 40-41 36.881624697902865 39.0 35.0 41.0 31.0 41.0 42-43 36.71750419550865 39.0 35.0 41.0 31.0 41.0 44-45 36.43423329900334 39.0 35.0 41.0 30.5 41.0 46-47 35.96156708915815 39.0 35.0 41.0 29.5 41.0 48-49 35.54386553035743 39.0 35.0 41.0 27.5 41.0 50-51 35.299901933832004 39.0 35.0 41.0 27.0 41.0 52-53 34.96312301764781 38.0 35.0 40.0 26.0 41.0 54-55 34.73916738130506 38.0 34.5 40.0 24.5 41.0 56-57 34.597700574035855 38.0 34.0 40.0 24.5 41.0 58-59 34.369061149795044 38.0 34.0 40.0 24.0 41.0 60-61 33.970594921075204 37.5 34.0 40.0 22.5 41.0 62-63 33.48225125454941 37.0 33.0 40.0 22.0 41.0 64-65 32.95650498742373 36.0 33.0 39.0 20.0 40.5 66-67 32.56041204203305 36.0 32.0 39.0 19.0 40.0 68-69 32.21885455433235 35.0 32.0 38.5 18.0 40.0 70-71 31.79722214243803 35.0 32.0 37.5 13.5 39.5 72-73 31.23898971331033 35.0 31.0 37.0 7.5 39.0 74-75 30.726562391009097 35.0 31.0 36.5 4.5 39.0 76-77 28.671246507162113 32.5 28.0 35.0 2.0 36.5 78-79 29.587065113473635 34.0 29.0 35.0 2.0 37.0 80-81 29.691628677994196 34.0 30.0 35.0 2.0 37.0 82-83 29.424762322896193 34.0 30.0 35.0 2.0 36.5 84-85 29.082216787779068 34.0 29.5 35.0 2.0 36.0 86-87 28.52506801032362 34.0 29.0 35.0 2.0 35.5 88-89 27.933276846126386 34.0 28.0 35.0 2.0 35.0 90-91 27.440061876059133 33.0 26.5 35.0 2.0 35.0 92-93 27.14451957835651 33.0 26.0 35.0 2.0 35.0 94-95 26.823952764111887 33.0 25.5 35.0 2.0 35.0 96-97 26.23765371564094 33.0 25.0 35.0 2.0 35.0 98-99 25.511963662176413 33.0 22.0 35.0 2.0 35.0 100-101 22.296640720847883 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13158676944586034 1101 2 0.08231944804142444 1101 3 0.10615485133066471 1101 4 0.15963307742102018 1101 5 0.10145812538384291 1101 6 0.07162227659944165 1101 7 0.07052366284049327 1101 8 0.044224475777859595 1101 9 0.17451036778184204 1101 10-11 0.20767516127735774 1101 12-13 0.2589062352484106 1101 14-15 0.2567584301526722 1101 16-17 0.1717489311327114 1101 18-19 0.09067275226751548 1101 20-21 0.11253827152528828 1101 22-23 0.17134787966270437 1101 24-25 0.14406120923498378 1101 26-27 0.22359558534595436 1101 28-29 0.21857258788018186 1101 30-31 0.10311569135817678 1101 32-33 0.1594464906466655 1101 34-35 0.20179833078930187 1101 36-37 0.14073634172741123 1101 38-39 0.24877898928644981 1101 40-41 0.2607766193600156 1101 42-43 0.4322984725696344 1101 44-45 0.6022067185046538 1101 46-47 0.5713767404969232 1101 48-49 0.6367346831019631 1101 50-51 0.6655669763844472 1101 52-53 0.451908613458869 1101 54-55 0.3113779551194682 1101 56-57 0.42118583805159204 1101 58-59 0.3391752305959841 1101 60-61 0.28442751275256484 1101 62-63 0.45032434309952407 1101 64-65 0.5536482441777295 1101 66-67 0.5665654835662863 1101 68-69 0.4140223881276057 1101 70-71 0.3335442863289835 1101 72-73 0.30880750837406623 1101 74-75 0.30914942102868537 1101 76-77 0.3502648605386618 1101 78-79 0.5857732266987767 1101 80-81 0.5495184910497741 1101 82-83 0.5683370054824834 1101 84-85 0.5122869296131505 1101 86-87 0.3460779085367527 1101 88-89 0.4992318082263445 1101 90-91 0.5431885629938016 1101 92-93 0.6118761795592746 1101 94-95 0.8782342358591144 1101 96-97 0.6217939280653368 1101 98-99 0.5167620117972866 1101 100-101 0.15116328333975915 1102 1 0.11711623556927009 1102 2 0.1374992971054283 1102 3 0.12286737285794658 1102 4 0.08768995144966851 1102 5 0.028639906652657032 1102 6 0.08480292374804321 1102 7 0.1452443332315525 1102 8 0.21753309649165686 1102 9 0.2828538521911881 1102 10-11 0.3235610701837004 1102 12-13 0.2767021216123595 1102 14-15 0.2537209831832925 1102 16-17 0.3158364544101744 1102 18-19 0.3704696607398077 1102 20-21 0.3129686421445754 1102 22-23 0.35818980990731575 1102 24-25 0.25117431322400563 1102 26-27 0.24869937134727138 1102 28-29 0.31029018494547245 1102 30-31 0.3490727628077508 1102 32-33 0.38582861215154907 1102 34-35 0.5012393215343209 1102 36-37 0.40854360429724323 1102 38-39 0.42924499368291436 1102 40-41 0.3731297787949117 1102 42-43 0.24402226529166882 1102 44-45 0.49180589101694494 1102 46-47 0.3026025004274828 1102 48-49 0.16634869718745904 1102 50-51 0.11417715406328455 1102 52-53 0.21396039094727826 1102 54-55 0.2768362937033544 1102 56-57 0.40127922795092275 1102 58-59 0.25331462937225524 1102 60-61 0.10122946875989669 1102 62-63 0.12543127943477117 1102 64-65 0.18641469242124487 1102 66-67 -0.07030777499912233 1102 68-69 0.19774933818430895 1102 70-71 0.29679126550876944 1102 72-73 0.34162436196054813 1102 74-75 0.2444380616245887 1102 76-77 0.3117424021580746 1102 78-79 0.13753233816459343 1102 80-81 0.2575184140416127 1102 82-83 0.3483360603823016 1102 84-85 0.5799464765251265 1102 86-87 0.5736562818443254 1102 88-89 0.5198822471728732 1102 90-91 0.6532454790268964 1102 92-93 0.594775991939386 1102 94-95 0.7507647240908675 1102 96-97 0.5118507740910196 1102 98-99 0.31635001812595576 1102 100-101 0.05526624325350937 1103 1 0.10213392685631106 1103 2 0.17276438998468535 1103 3 0.13731756082787427 1103 4 0.06677138665533278 1103 5 0.12836071602903587 1103 6 0.17232641998864295 1103 7 0.08733992146684955 1103 8 0.11264311418294426 1103 9 0.17948421486257615 1103 10-11 0.21188136540705926 1103 12-13 0.2029239261236384 1103 14-15 0.2493361700475134 1103 16-17 0.29561586617488445 1103 18-19 0.20199124963852455 1103 20-21 0.3499821317509486 1103 22-23 0.3237194428131076 1103 24-25 0.2765467977441247 1103 26-27 0.27810422539415214 1103 28-29 0.3479636424863841 1103 30-31 0.2915553479470674 1103 32-33 0.2805689095863144 1103 34-35 0.40828059198987177 1103 36-37 0.45587816312077223 1103 38-39 0.34223288620835035 1103 40-41 0.42312839666311675 1103 42-43 0.5577178092987438 1103 44-45 0.648211380844451 1103 46-47 0.6762169132978926 1103 48-49 0.507127113817269 1103 50-51 0.3986095125329854 1103 52-53 0.49257742986810626 1103 54-55 0.45591220038181746 1103 56-57 0.586032240998243 1103 58-59 0.49477996550670866 1103 60-61 0.410063364160159 1103 62-63 0.44504925820523056 1103 64-65 0.5765628569502184 1103 66-67 0.5457809302198058 1103 68-69 0.4469822550352589 1103 70-71 0.6325767310107686 1103 72-73 0.7616714926549264 1103 74-75 0.6571218851540017 1103 76-77 0.7246059028214944 1103 78-79 0.7765817079124915 1103 80-81 0.795440655306539 1103 82-83 0.9359044759064084 1103 84-85 0.890425108767495 1103 86-87 0.5020514886112402 1103 88-89 0.8503706925510244 1103 90-91 1.0566621088367185 1103 92-93 0.9334501129035608 1103 94-95 0.8717807291771145 1103 96-97 0.7896903638742963 1103 98-99 1.0307635796031782 1103 100-101 0.6634318779284314 1104 1 0.10789417319311667 1104 2 0.14096690113354526 1104 3 0.04870068357155333 1104 4 0.19578962301124392 1104 5 0.15581479332782777 1104 6 0.1352664019381038 1104 7 0.12313241987415324 1104 8 0.15761683471809107 1104 9 0.17257590099551123 1104 10-11 0.12777546447267696 1104 12-13 0.17156111023916054 1104 14-15 0.2259716685610016 1104 16-17 0.03966316921162161 1104 18-19 0.10981237673556876 1104 20-21 0.16562770405928973 1104 22-23 0.22927263882075266 1104 24-25 0.18125221556107363 1104 26-27 0.2316141266470737 1104 28-29 0.3287733287254966 1104 30-31 0.30674899309465786 1104 32-33 0.4920638527801984 1104 34-35 0.3050855797792167 1104 36-37 0.2715851205427313 1104 38-39 0.3964740998418179 1104 40-41 0.42102139411480266 1104 42-43 0.5695800677400271 1104 44-45 0.6811652325114679 1104 46-47 0.5560497467020085 1104 48-49 0.4823739815174193 1104 50-51 0.5025776453637363 1104 52-53 0.7128305356230484 1104 54-55 0.6933796828621723 1104 56-57 0.7160542069145777 1104 58-59 0.5300681105481146 1104 60-61 0.48617592786795427 1104 62-63 0.4454806130576401 1104 64-65 0.6934533857249008 1104 66-67 0.8365588952867 1104 68-69 0.8549875374731428 1104 70-71 0.6986685896856422 1104 72-73 0.6512098102382851 1104 74-75 0.569347808917005 1104 76-77 0.6560038907170167 1104 78-79 0.9945784959778976 1104 80-81 0.8725685619746208 1104 82-83 0.8044159034918827 1104 84-85 0.55825698655471 1104 86-87 0.34374259871954393 1104 88-89 0.6861406277814339 1104 90-91 0.8369233107904215 1104 92-93 0.5611195358171308 1104 94-95 0.47230797753132947 1104 96-97 0.5023267383699448 1104 98-99 0.44384723589963215 1104 100-101 0.18267694057442796 1105 1 0.03208189882488455 1105 2 -0.009755120527103145 1105 3 -0.009061049280425948 1105 4 0.027017059834349766 1105 5 -0.01386029692488222 1105 6 0.07118697955542785 1105 7 0.05273530796079484 1105 8 0.07638939789498522 1105 9 0.0019982836669925064 1105 10-11 0.025338641367980586 1105 12-13 0.010460027206669054 1105 14-15 0.0016936902216500016 1105 16-17 0.069897826251335 1105 18-19 0.08454161861283183 1105 20-21 0.10380459936614272 1105 22-23 0.11600549080631595 1105 24-25 0.13793091592208384 1105 26-27 0.18242640101530583 1105 28-29 0.08238343702874573 1105 30-31 0.045016141109094576 1105 32-33 -0.10586033494543301 1105 34-35 -0.09383138772981425 1105 36-37 -0.21307191916846335 1105 38-39 -0.13691940196324026 1105 40-41 0.07896363238194937 1105 42-43 0.07364143958118063 1105 44-45 0.14686920363060807 1105 46-47 0.10884758063702549 1105 48-49 0.08074943278095503 1105 50-51 -0.04886639795756054 1105 52-53 -0.12516585426864424 1105 54-55 -0.18793800666851013 1105 56-57 -0.09441510716484203 1105 58-59 -0.05747701580567366 1105 60-61 -0.2516399566085781 1105 62-63 -0.2747359934405509 1105 64-65 -0.3394888525422459 1105 66-67 -0.22436168233063114 1105 68-69 -0.14095109068569656 1105 70-71 -0.042757403094139335 1105 72-73 -0.3045663630469164 1105 74-75 -0.13958720913353417 1105 76-77 -0.2830755316656557 1105 78-79 -0.19314713579105458 1105 80-81 0.08691874248003728 1105 82-83 -0.06336387990162251 1105 84-85 -0.21295239972687696 1105 86-87 -0.10571588503497154 1105 88-89 0.07873990575977174 1105 90-91 0.36760923136803925 1105 92-93 0.1807043733622571 1105 94-95 0.11445599197176293 1105 96-97 -0.009857377153519309 1105 98-99 0.44971366189241024 1105 100-101 0.5373700813686533 1106 1 0.11827954594311052 1106 2 0.059610081058316666 1106 3 0.2123831202684343 1106 4 0.09829776468906459 1106 5 0.1603305843959646 1106 6 0.20521540600262966 1106 7 0.20905552204273903 1106 8 0.18163478427755564 1106 9 0.2208895007531524 1106 10-11 0.2534634046913027 1106 12-13 0.2068574925572051 1106 14-15 0.3403448855966644 1106 16-17 0.1244028879766077 1106 18-19 0.2253516208849291 1106 20-21 0.2805405439270032 1106 22-23 0.28096440373454357 1106 24-25 0.15124792382393082 1106 26-27 0.20482548207865392 1106 28-29 0.19662095943414215 1106 30-31 0.11326580660605856 1106 32-33 0.11128736392608829 1106 34-35 0.30867202041439157 1106 36-37 0.16509954835319007 1106 38-39 0.1665143033206533 1106 40-41 0.19713472122091247 1106 42-43 0.1624393744982484 1106 44-45 0.07490371424633935 1106 46-47 0.006543296473537907 1106 48-49 -0.0853502617483457 1106 50-51 5.76938489381007E-4 1106 52-53 0.04258899918114878 1106 54-55 0.1761212193040791 1106 56-57 0.1185323695461662 1106 58-59 0.18575718681481135 1106 60-61 -0.12207683020429982 1106 62-63 0.05155121213365277 1106 64-65 0.05640654267625678 1106 66-67 0.041156418701916664 1106 68-69 0.12320217482135831 1106 70-71 0.16198528201858053 1106 72-73 0.22714673432502508 1106 74-75 0.23657092875745533 1106 76-77 0.29162436230319244 1106 78-79 0.32877859191084724 1106 80-81 0.39332668471830345 1106 82-83 0.30266134036547854 1106 84-85 0.16051393538205616 1106 86-87 0.20708863896089014 1106 88-89 0.4570076217982475 1106 90-91 0.5041117180510319 1106 92-93 0.5103571213363054 1106 94-95 0.46992385443421014 1106 96-97 0.6902874689750824 1106 98-99 0.8919093272378937 1106 100-101 1.0023356212439403 1107 1 -0.06983042101300896 1107 2 0.02707530813892589 1107 3 0.045271385849005696 1107 4 0.04227686251431351 1107 5 -0.07917029933568642 1107 6 0.0917711579429934 1107 7 -0.04315380708966643 1107 8 -0.07104522801784441 1107 9 0.022165148182644145 1107 10-11 0.12690726851977985 1107 12-13 0.13672570127156547 1107 14-15 0.09350998972331581 1107 16-17 0.05030391003147372 1107 18-19 0.05616503823680574 1107 20-21 0.10293449612864691 1107 22-23 0.11021688202548319 1107 24-25 -0.03709258182426822 1107 26-27 0.06620448768841669 1107 28-29 0.03382702260174142 1107 30-31 0.031170017779494685 1107 32-33 0.018500343254451934 1107 34-35 0.13895231704083955 1107 36-37 0.24101401849842574 1107 38-39 0.2798672964813278 1107 40-41 0.23222463131082094 1107 42-43 0.2964592008257725 1107 44-45 0.23723177080517388 1107 46-47 0.37977583316761354 1107 48-49 0.5510614035094719 1107 50-51 0.37647027592117865 1107 52-53 0.29631123848031393 1107 54-55 0.15406635109536637 1107 56-57 0.1545492372938071 1107 58-59 0.09503060570361299 1107 60-61 -0.042320048524437937 1107 62-63 0.033189765887591705 1107 64-65 0.14171066163422097 1107 66-67 0.14319380439314244 1107 68-69 0.11769806354801204 1107 70-71 0.17280619187794954 1107 72-73 0.10974010249531574 1107 74-75 0.20682183170066892 1107 76-77 0.3049715919965763 1107 78-79 0.4462284497453197 1107 80-81 0.28481020120182166 1107 82-83 0.2842566681146508 1107 84-85 0.34429169608289456 1107 86-87 0.4718911441480138 1107 88-89 0.5527436165727053 1107 90-91 0.6805122406054025 1107 92-93 0.5425364804387414 1107 94-95 0.7338474308577858 1107 96-97 0.8269728989297427 1107 98-99 0.8204375298045612 1107 100-101 0.7825457956848823 1108 1 0.0704971732445614 1108 2 0.07220613965186118 1108 3 0.04261146275578653 1108 4 9.70113296027364E-4 1108 5 0.09458063861668364 1108 6 0.1368460134505014 1108 7 0.04790569115275645 1108 8 0.10561221087442618 1108 9 0.11122297281336557 1108 10-11 0.16672711080915548 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-1.1054549336811021 2316 56-57 -0.8806550880031168 2316 58-59 -1.0367479072703105 2316 60-61 -0.9237794025977095 2316 62-63 -0.8983587445307464 2316 64-65 -1.0240698379882254 2316 66-67 -0.8116382729265652 2316 68-69 -0.8504484905187724 2316 70-71 -0.7577810836267176 2316 72-73 -0.7456280114490355 2316 74-75 -0.709734304996477 2316 76-77 -0.8339825034712511 2316 78-79 -0.7366493112393542 2316 80-81 -0.7101274876483181 2316 82-83 -0.9886103154752632 2316 84-85 -1.0730773707126708 2316 86-87 -0.9577958951096903 2316 88-89 -1.151531667230099 2316 90-91 -1.4850615573739496 2316 92-93 -1.367432667534196 2316 94-95 -1.257846921301681 2316 96-97 -1.2967387571927311 2316 98-99 -1.4924387972778064 2316 100-101 -0.5810961658472173 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 12.0 5 55.0 6 160.0 7 329.0 8 621.0 9 994.0 10 1680.0 11 2681.0 12 3975.0 13 5763.0 14 6431.0 15 8877.0 16 11628.0 17 16793.0 18 15500.0 19 9330.0 20 8090.0 21 8431.0 22 9301.0 23 9904.0 24 10906.0 25 12546.0 26 14492.0 27 17178.0 28 20750.0 29 24909.0 30 30122.0 31 35754.0 32 44080.0 33 56161.0 34 77089.0 35 119193.0 36 165449.0 37 255424.0 38 197915.0 39 16033.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.53252823282743 19.17717477527295 14.26280310585788 19.027493886041743 2 13.896263227648914 23.715107524013902 39.78474681284954 22.603882435487645 3 22.072027343637803 30.44564713412908 29.466298474024775 18.016027048208343 4 11.721820337856412 17.17216562103786 38.63733161546573 32.46868242564 5 11.537915498968049 41.596714167894206 32.81269361913398 14.052676714003768 6 26.39063160356649 37.55499296303439 18.78291268828499 17.27146274511413 7 25.34891450189362 37.07623311025674 20.617365507789902 16.95748688005974 8 24.497092892049256 32.18950158588175 20.902701127966093 22.4107043941029 9 27.496276357847137 19.65836865493429 21.601637992228564 31.243716994990006 10-11 23.237537595450387 27.38265911133177 26.452302503354357 22.927500789863487 12-13 29.672442586156667 25.234845904814268 26.184323363956786 18.908388145072276 14-15 25.927506450363385 25.49173762613497 28.967671041315153 19.61308488218649 16-17 19.055235391025864 29.696546941773367 30.899321533862782 20.348896133337984 18-19 18.930791545793618 29.91736181492165 33.14775986508721 18.00408677419752 20-21 20.402768830550688 28.034614485070552 31.071424175156842 20.49119250922191 22-23 19.463918625596502 27.605995576764474 30.005580334245607 22.92450546339342 24-25 20.602085980747002 28.167674479007452 31.305180238863215 19.925059301382326 26-27 22.044072963918108 25.584148340544786 32.995225873427174 19.37655282210993 28-29 19.590953293423002 26.292360276226546 34.721496186087734 19.395190244262718 30-31 19.280013786708135 29.592758695678935 32.697681289057215 18.42954622855572 32-33 18.725344975442425 29.116624882546272 31.783655365122087 20.37437477688921 34-35 19.791624778522852 27.11610695899872 31.907645567625725 21.184622694852703 36-37 21.656845628714695 26.925978184408965 32.519455340748564 18.89772084612777 38-39 22.69240633328751 25.463599189976055 33.26568087764905 18.57831359908739 40-41 19.48759156379825 27.32429128214557 34.442310857068755 18.74580629698743 42-43 18.721978890456388 29.388486345107633 32.23308803193432 19.656446732501657 44-45 19.5099703830508 29.470341608407963 30.204118142822793 20.81556986571844 46-47 19.620774633728416 28.787924793383684 29.007773910745687 22.583526662142212 48-49 20.74000976558493 28.19106900329486 29.802964962886673 21.265956268233538 50-51 21.857061379573516 29.122779662964227 29.801898134280897 19.218260823181367 52-53 19.482136775633634 29.59546679906283 30.924694209992904 19.997702215310632 54-55 17.915786191134657 29.00243318985856 31.547024573986615 21.534756045020167 56-57 20.31369697705784 30.638509492111414 28.14344255064053 20.904350980190216 58-59 19.750770890992015 28.62236702002162 29.6687681951816 21.958093893804765 60-61 19.84333678692695 27.852240903542157 30.632705189622804 21.671717119908088 62-63 22.48494925090928 27.408981538623145 29.888703239275728 20.217365971191846 64-65 21.07560942584105 28.151103962447632 31.202562029928643 19.57072458178267 66-67 19.364170150956248 28.583538834612842 31.168136291457575 20.88415472297333 68-69 19.04191754359042 30.347812457172772 30.238790243229914 20.37147975600689 70-71 19.322284540539297 30.011583301499428 28.441334045099786 22.224798112861485 72-73 19.422189214362795 28.578327787192315 28.104696918096284 23.894786080348606 74-75 19.925609220681704 29.442582053480937 28.80519299339797 21.826615732439386 76-77 20.840382927657796 27.35716911870544 30.559569860280263 21.242878093356506 78-79 19.524235473692418 27.38384903554591 31.728877819402328 21.363037671359344 80-81 18.82416323944743 30.44659097856598 30.00278609022911 20.726459691757483 82-83 18.61915449729805 29.59817490244673 28.915896977182175 22.86677362307304 84-85 19.089256625621122 27.93552252034155 28.84002905056357 24.135191803473756 86-87 20.27905677659439 27.866123394519903 29.709935390593234 22.144884438292472 88-89 21.227627275463636 26.000392823598734 30.651070114894726 22.1209097860429 90-91 19.29197862371865 27.082305601102906 31.491554820575452 22.13416095460299 92-93 18.187704389999713 29.04051076471095 30.55963366746952 22.212151177819813 94-95 17.928069432545193 29.25681375783299 29.419176998499875 23.395939811121945 96-97 18.175189669816547 27.823054166170863 28.447928506070664 25.553827657941923 98-99 19.387599348413914 27.257635005108465 28.636018595643236 24.718747050834384 100-101 20.250450735825723 27.269209716690888 29.411281977214145 23.069057570269244 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4453.0 1 3035.0 2 3180.5 3 4114.5 4 2810.0 5 2485.0 6 4152.5 7 7916.0 8 11133.5 9 10801.5 10 7884.0 11 5585.0 12 4944.5 13 4651.0 14 4387.0 15 4014.5 16 3666.5 17 3546.0 18 3541.0 19 3551.0 20 3617.0 21 3815.0 22 4133.5 23 4653.5 24 5101.0 25 5604.0 26 6663.5 27 7708.0 28 8189.0 29 9446.0 30 11452.5 31 13381.0 32 16246.0 33 19693.0 34 22615.0 35 25022.0 36 29875.5 37 34993.5 38 35884.5 39 35554.5 40 34351.5 41 32093.5 42 30338.0 43 30152.5 44 32057.5 45 33516.5 46 36625.5 47 49766.5 48 114260.0 49 115931.5 50 63367.0 51 49322.0 52 29183.5 53 21678.5 54 19461.5 55 17689.5 56 16199.5 57 14914.0 58 13482.0 59 12129.0 60 10676.0 61 9248.5 62 7631.0 63 5561.5 64 3962.0 65 3095.5 66 2438.5 67 1904.5 68 1393.5 69 954.5 70 723.5 71 585.0 72 512.0 73 565.0 74 482.5 75 305.0 76 235.0 77 167.5 78 118.5 79 85.0 80 49.5 81 31.5 82 23.0 83 11.0 84 6.0 85 3.5 86 1.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2718771669956055 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0023798484282742407 16-17 6.154780417950622E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2309560835901246E-4 26-27 0.0014361154308551451 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0034466770340523486 36-37 0.021500699593374174 38-39 0.006770258459745684 40-41 0.018095054428774827 42-43 0.005252079289984531 44-45 4.923824334360498E-4 46-47 1.641274778120166E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 4.103186945300415E-5 58-59 6.154780417950622E-4 60-61 0.0022567528199152285 62-63 0.0017233385170261743 64-65 0.0 66-67 0.0 68-69 4.923824334360498E-4 70-71 4.103186945300415E-5 72-73 0.0 74-75 0.0 76-77 0.22764481172526702 78-79 0.0 80-81 9.437329974190954E-4 82-83 0.0 84-85 0.0 86-87 0.10257967363251037 88-89 0.3511917706482625 90-91 0.3546794795517678 92-93 0.0 94-95 8.20637389060083E-5 96-97 0.0 98-99 0.0 100-101 1.641274778120166E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1218565.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.867829645437283 #Duplication Level Percentage of deduplicated Percentage of total 1 69.32613531602217 20.70621199595484 2 14.202348553556988 8.483866543255255 3 5.840679596892506 5.233452696406999 4 3.000573653068699 3.5848249083377333 5 1.7787735068533668 2.6564052040256714 6 1.1313442586953553 2.027447855343384 7 0.7785431946951719 1.6277376857538932 8 0.5621475295235729 1.3432101317930802 9 0.4262861244514878 1.145901721079764 >10 2.521743539585215 14.386416818437956 >50 0.24652927738951202 5.098332946353013 >100 0.15325411494616978 8.272051438355277 >500 0.014857670028393784 3.0570571283159396 >1k 0.012381391690328153 8.171546575570531 >5k 0.003026562413191326 6.8177304698749195 >10k+ 0.001375710187814239 7.387805881141792 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 26233 2.1527780627213158 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 22475 1.8443825319125366 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15986 1.3118709301514486 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14161 1.1621046066479834 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11044 0.9063119324779557 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9767 0.8015165378949831 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9756 0.800613836767017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9724 0.7979877971220246 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8631 0.7082921304977576 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7057 0.5791238054597005 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6960 0.5711636227858178 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6762 0.5549150024824281 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 6597 0.5413744855629368 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6286 0.5158526627631682 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6226 0.5109288384288077 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 5196 0.42640318735561916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4609 0.37823177261779223 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 4437 0.3641168095259588 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4187 0.3436008747994567 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 4180 0.3430264286271147 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4150 0.3405645164599344 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3521 0.2889464246880552 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3501 0.28730514990993505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3484 0.2859100663485329 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 3295 0.2704000196952973 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3112 0.2553823554754978 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2609 0.21410429480577564 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2489 0.20425664613705463 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2452 0.20122028779753234 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2440 0.20023552293066021 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2437 0.19998933171394223 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2301 0.1888286632227251 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2280 0.1871053247056989 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2265 0.1858743686221088 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2214 0.18168911793790238 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2103 0.17258004291933546 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2010 0.1649481152010767 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1984 0.16281445798952046 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1940 0.15920365347765608 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1897 0.15567491270469774 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1816 0.14902774985331108 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1789 0.14681202890284883 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1677 0.13762089014537593 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1674 0.1373746989286579 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1669 0.13696438023412785 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1635 0.13417421311132358 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1627 0.1335177032000755 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1531 0.1256395842650987 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1524 0.12506513809275663 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1503 0.12334179957573047 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1379 0.11316589595138545 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1343 0.11021160135076914 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1316 0.10799588040030692 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.103186945300415E-5 0.0 12-13 0.0 0.0 0.0 2.8722308617102905E-4 0.0 14-15 0.0 0.0 0.0 4.103186945300415E-4 0.0 16-17 0.0 0.0 0.0 7.385736501540746E-4 0.0 18-19 0.0 0.0 0.0 8.20637389060083E-4 0.0 20-21 0.0 0.0 0.0 0.0011488923446841162 0.0 22-23 0.0 0.0 0.0 0.0021746890810092198 0.0 24-25 0.0 0.0 0.0 0.004800728726001485 0.0 26-27 0.0 0.0 0.0 0.007960182673882803 0.0 28-29 0.0 0.0 0.0 0.015510046653235568 0.0 30-31 0.0 0.0 0.0 0.035328439599036573 0.0 32-33 0.0 0.0 0.0 0.06860528572542293 0.0 34-35 0.0 0.0 0.0 0.12432656444260257 0.0 36-37 0.0 0.0 0.0 0.21151928703023637 0.0 38-39 0.0 0.0 0.0 0.31455031122672983 0.0 40-41 0.0 0.0 0.0 0.4422004570950257 0.0 42-43 0.0 0.0 0.0 0.5643933643260721 0.0 44-45 0.0 0.0 0.0 0.692946211322334 0.0 46-47 0.0 0.0 0.0 0.8633105332912072 0.0 48-49 0.0 0.0 0.0 1.057268180195558 0.0 50-51 0.0 0.0 0.0 1.2490921698883524 0.0 52-53 0.0 0.0 0.0 1.434966538510461 0.0 54-55 0.0 0.0 0.0 1.6340942009658903 0.0 56-57 0.0 0.0 0.0 1.8560355828371895 0.0 58-59 0.0 0.0 0.0 2.156183707885915 0.0 60-61 0.0 0.0 0.0 2.551648865673969 0.0 62-63 0.0 0.0 0.0 2.8943880712149124 0.0 64-65 0.0 0.0 0.0 3.194946514958168 0.0 66-67 0.0 0.0 0.0 3.5276329124831256 0.0 68-69 0.0 0.0 0.0 3.9029103905003018 0.0 70-71 0.0 0.0 0.0 4.345890453114935 0.0 72-73 0.0 4.103186945300415E-5 0.0 4.825840230106724 0.0 74-75 0.0 8.20637389060083E-5 0.0 5.303081903714615 0.0 76-77 0.0 8.20637389060083E-5 0.0 5.7542683402198485 0.0 78-79 0.0 8.20637389060083E-5 0.0 6.2704902898080945 0.0 80-81 0.0 8.20637389060083E-5 0.0 6.850680923873573 0.0 82-83 0.0 8.20637389060083E-5 0.0 7.447899783762048 0.0 84-85 0.0 8.20637389060083E-5 0.0 8.207317623598248 0.0 86-87 0.0 8.20637389060083E-5 0.0 9.026765088444193 0.0 88-89 0.0 8.20637389060083E-5 0.0 9.724019646059094 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 45 2.0621337E-7 63.269352 2 ATCACGC 55 8.2484985E-7 51.76583 3 CCGTAGG 65 1.3361537E-4 36.50155 2 GTAATAC 130 8.370989E-9 32.851395 3 TACCTGG 790 0.0 31.234238 2 CGTAGGG 80 4.5204768E-4 29.657507 3 TCACGCA 100 4.9875973E-5 28.471207 4 TCGACCG 45 9.003406E-4 26.36223 18-19 GTTCGAC 55 9.546273E-5 25.882915 16-17 ACTATAC 315 0.0 25.609024 3 CATGGGG 2535 0.0 23.959991 4 GGATATG 480 0.0 23.79337 1 GGGCGAT 100 0.0016634604 23.726006 7 GACATAT 305 0.0 23.403316 1 AGTACTT 10305 0.0 23.207972 12-13 GAGTACT 9830 0.0 23.050188 12-13 CATGGGT 845 0.0 23.024054 4 TCTAGAC 165 2.4515175E-6 23.007036 3 ATATGAG 435 0.0 22.90787 3 CCCGTAG 105 0.0021731297 22.660353 1 >>END_MODULE