FastQCFastQC Report
Fri 27 May 2016
SRR939265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293034
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71950.21849151876354753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51720.15705880959625684No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36950.11220655480629717No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121700.032.3644521
GGTATCA79600.029.9871851
ATCAACG144100.027.20943
GTACATG123900.026.9638651
TCAACGC145700.026.9106034
CAACGCA148150.026.401575
TACATGG124900.026.2296832
TATCAAC150850.026.0547352
AACGCAG151550.025.8560836
ACATGGG125850.025.3159083
ACGCAGA171400.022.7234467
CATGGGG58000.022.3975224
CGCAGAG173250.022.2623448
GAGTACT80350.020.31359512-13
GCAGAGT185300.020.225899
AGTACTT85150.019.41888212-13
CATGGGA74900.019.0529444
AGAGTAC152100.019.00147610-11
GTACTTT87800.018.54035614-15
GAGTACA104750.016.8527071
Produced by FastQC (version 0.11.5)