FastQCFastQC Report
Fri 27 May 2016
SRR939265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3293034
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76430.2320959941500756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56140.17048108218742958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.12210624002060107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128050.032.5259321
GGTATCA82450.032.0408171
ATCAACG152550.026.9077723
TCAACGC154800.026.5166684
CAACGCA157250.026.1638855
TATCAAC158750.025.9764542
AACGCAG161550.025.4674786
GTACATG125250.024.9302921
TACATGG125650.024.3974152
ACATGGG127000.023.091843
ACGCAGA184600.022.236087
CGCAGAG186450.021.8881918
CATGGGG57700.021.1364044
GAGTACT89000.021.0628512-13
GCAGAGT200000.020.050589
AGTACTT93200.019.96090312-13
TAAGGTG14100.019.5201285
GTACTTT97000.019.0567614-15
AGAGTAC164300.018.90509210-11
CATGGGA73250.017.7507514
Produced by FastQC (version 0.11.5)