##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939265_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3293034 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34947346428856 34.0 31.0 34.0 31.0 34.0 2 32.560636179280266 34.0 31.0 34.0 31.0 34.0 3 32.65438741294502 34.0 31.0 34.0 31.0 34.0 4 36.16396368819757 37.0 35.0 37.0 35.0 37.0 5 36.10492360540462 37.0 35.0 37.0 35.0 37.0 6 36.130804905142185 37.0 36.0 37.0 35.0 37.0 7 36.07266794087155 37.0 35.0 37.0 35.0 37.0 8 36.04448784919925 37.0 35.0 37.0 35.0 37.0 9 37.76654264729729 39.0 38.0 39.0 35.0 39.0 10-11 37.69152216466638 39.0 37.0 39.0 35.0 39.0 12-13 37.621893518257025 39.0 37.0 39.0 35.0 39.0 14-15 38.954129838926654 40.0 38.0 41.0 35.0 41.0 16-17 38.956027329204616 40.0 38.0 41.0 35.5 41.0 18-19 39.039151129323294 40.0 38.5 41.0 35.0 41.0 20-21 39.017138905945096 40.0 38.5 41.0 35.0 41.0 22-23 38.948063396855304 40.0 38.0 41.0 35.0 41.0 24-25 38.88771555349869 40.0 38.0 41.0 35.0 41.0 26-27 38.74650641323473 40.0 38.0 41.0 34.5 41.0 28-29 38.59296108087557 40.0 38.0 41.0 34.0 41.0 30-31 38.41453443845402 40.0 38.0 41.0 34.0 41.0 32-33 38.3769012102517 40.0 38.0 41.0 34.0 41.0 34-35 38.29671968160669 40.0 38.0 41.0 34.0 41.0 36-37 38.157695608366026 40.0 38.0 41.0 33.5 41.0 38-39 38.041188763918015 40.0 38.0 41.0 33.0 41.0 40-41 37.87652389862966 40.0 37.5 41.0 33.0 41.0 42-43 37.70815257297677 40.0 37.0 41.0 33.0 41.0 44-45 37.495599802492166 40.0 37.0 41.0 32.5 41.0 46-47 37.23181934957246 40.0 37.0 41.0 31.5 41.0 48-49 36.961566446019084 40.0 36.5 41.0 31.0 41.0 50-51 36.738049774159634 39.5 36.0 41.0 31.0 41.0 52-53 36.490641457087904 39.0 36.0 41.0 30.0 41.0 54-55 36.25246596299947 39.0 35.0 40.5 30.0 41.0 56-57 35.95707226223598 39.0 35.0 40.0 29.0 41.0 58-59 35.65218397380653 38.0 35.0 40.0 28.5 41.0 60-61 35.32581670884662 38.0 34.5 40.0 28.0 41.0 62-63 34.99221447455447 38.0 34.0 40.0 28.0 41.0 64-65 34.698859774906666 37.0 34.0 40.0 27.5 41.0 66-67 34.300616543892346 36.5 34.0 39.0 26.5 41.0 68-69 33.84609360243471 36.0 33.0 39.0 26.0 40.0 70-71 33.37698988227878 35.5 33.0 38.5 26.0 40.0 72-73 32.89864908774097 35.0 32.5 37.5 25.0 40.0 74-75 32.383445023646885 35.0 32.0 37.0 24.0 39.0 76-77 30.403484901765363 33.5 29.5 35.5 20.5 37.5 78-79 31.369404931743794 34.0 31.0 36.0 22.0 38.0 80-81 31.377315721611133 34.0 31.0 36.0 22.0 37.0 82-83 31.037548352066818 34.0 31.0 35.0 20.5 37.0 84-85 30.605955480568984 34.0 31.0 35.0 19.0 36.5 86-87 30.031353305189075 34.0 30.0 35.0 16.5 36.0 88-89 29.470255697329577 34.0 29.5 35.0 7.5 35.5 90-91 29.016518657262573 34.0 29.0 35.0 4.5 35.0 92-93 28.68176900086668 34.0 29.0 35.0 2.0 35.0 94-95 28.20144644725806 33.0 29.0 35.0 2.0 35.0 96-97 27.522069769094397 33.0 27.0 35.0 2.0 35.0 98-99 26.580883464914116 33.0 25.5 34.0 2.0 35.0 100-101 22.268817601032968 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23576606978715375 1101 2 0.17959218126521392 1101 3 0.1625361334866966 1101 4 0.08950555868140242 1101 5 0.14884234870640967 1101 6 0.16214573126269016 1101 7 0.15306017472298805 1101 8 0.1277149670709079 1101 9 0.22070282535828767 1101 10-11 0.22904482753927624 1101 12-13 0.2358174289077013 1101 14-15 0.2784905569048348 1101 16-17 0.2681598664644653 1101 18-19 0.2949182574723892 1101 20-21 0.3152891505063664 1101 22-23 0.2773334823753473 1101 24-25 0.25927795421017663 1101 26-27 0.3059659726098616 1101 28-29 0.36589730167396084 1101 30-31 0.2718665823001558 1101 32-33 0.41191200528337646 1101 34-35 0.46798884471817104 1101 36-37 0.40875703220099524 1101 38-39 0.4446869580016042 1101 40-41 0.5401817072842903 1101 42-43 0.5130604324977384 1101 44-45 0.5318824705239109 1101 46-47 0.4626707022573697 1101 48-49 0.4102403476414551 1101 50-51 0.4642787405734765 1101 52-53 0.41195924502930126 1101 54-55 0.34064440156171827 1101 56-57 0.3796561393639024 1101 58-59 0.4585348859986027 1101 60-61 0.4258617854492073 1101 62-63 0.49606537316369526 1101 64-65 0.46466812782048095 1101 66-67 0.4047530771572312 1101 68-69 0.38167443341063745 1101 70-71 0.48680549557037267 1101 72-73 0.4606527829047167 1101 74-75 0.4483106464419748 1101 76-77 0.7145513526874687 1101 78-79 0.8442530921433651 1101 80-81 0.8530167762367817 1101 82-83 0.8252045006039701 1101 84-85 0.7383989032790801 1101 86-87 0.7305797763402673 1101 88-89 0.7453442193092776 1101 90-91 0.9166099298932053 1101 92-93 1.0230901188861914 1101 94-95 0.8779066172237684 1101 96-97 0.6915632608328721 1101 98-99 0.5310030488157373 1101 100-101 0.5896959216116677 1102 1 0.22033920833140286 1102 2 0.22688341750465213 1102 3 0.2464583437064789 1102 4 0.16538695129046488 1102 5 0.23826039361417628 1102 6 0.21098213570522262 1102 7 0.25700210156870895 1102 8 0.24191967738682507 1102 9 0.22946938260466965 1102 10-11 0.22847238117444135 1102 12-13 0.2815044169833314 1102 14-15 0.36786630195906866 1102 16-17 0.34712355501169156 1102 18-19 0.3037330575269195 1102 20-21 0.25756890285995127 1102 22-23 0.3330371456824963 1102 24-25 0.37064437931321237 1102 26-27 0.3381091418826898 1102 28-29 0.38262671894977984 1102 30-31 0.3756747926667643 1102 32-33 0.48022976457973243 1102 34-35 0.4723165392363171 1102 36-37 0.46291342979164796 1102 38-39 0.4277144039644085 1102 40-41 0.5483408596092119 1102 42-43 0.5382284957587444 1102 44-45 0.5670511280803794 1102 46-47 0.5887871911597742 1102 48-49 0.5583936618874219 1102 50-51 0.5069302771768349 1102 52-53 0.3694945619111323 1102 54-55 0.27549021684770025 1102 56-57 0.3075338403493859 1102 58-59 0.2870453368591015 1102 60-61 0.36222987108369153 1102 62-63 0.3736401582559594 1102 64-65 0.3632306319718097 1102 66-67 0.32221382743777127 1102 68-69 0.37967148290846353 1102 70-71 0.10729138454899356 1102 72-73 0.3683600958851798 1102 74-75 0.381545221852825 1102 76-77 0.28233179896840355 1102 78-79 0.4207130488646733 1102 80-81 0.5653291798822657 1102 82-83 0.5726425627812581 1102 84-85 0.5786606729511341 1102 86-87 0.49989437887895605 1102 88-89 0.6006128233791515 1102 90-91 0.6759080319931634 1102 92-93 0.5864430639697922 1102 94-95 0.560004864751324 1102 96-97 0.3924090831105609 1102 98-99 0.10059906773530969 1102 100-101 -0.09906560160256461 1103 1 0.12802723817203088 1103 2 0.2162038651140108 1103 3 0.21752582420834443 1103 4 0.141306245966625 1103 5 0.191914513654865 1103 6 0.12292923641732756 1103 7 0.15310484826250814 1103 8 0.1358352419849922 1103 9 0.19325608996309995 1103 10-11 0.1551565113880713 1103 12-13 0.21045660416543654 1103 14-15 0.2403513816470948 1103 16-17 0.13924749533044434 1103 18-19 0.13281172826276588 1103 20-21 0.2147376041146174 1103 22-23 0.20018227962620472 1103 24-25 0.30071609854007164 1103 26-27 0.24733023377824992 1103 28-29 0.23215503363272205 1103 30-31 0.2577617713035991 1103 32-33 0.3409394966923074 1103 34-35 0.3421778481545985 1103 36-37 0.3456728397611286 1103 38-39 0.39187767965108833 1103 40-41 0.2867160715454631 1103 42-43 0.377986549336228 1103 44-45 0.4027381406270081 1103 46-47 0.5077995682367487 1103 48-49 0.43602557100916073 1103 50-51 0.4326859570683297 1103 52-53 0.32137333437638205 1103 54-55 0.37119079331429816 1103 56-57 0.43835717029173793 1103 58-59 0.42546615747626504 1103 60-61 0.5237844658615742 1103 62-63 0.4397715587307047 1103 64-65 0.427291839160695 1103 66-67 0.3830159637551631 1103 68-69 0.2703359454381342 1103 70-71 0.3694051519277721 1103 72-73 0.4566493464785992 1103 74-75 0.2894584127649935 1103 76-77 0.32089843172526855 1103 78-79 0.2883149478134719 1103 80-81 0.4755751954807721 1103 82-83 0.41826463806101444 1103 84-85 0.4023593843787374 1103 86-87 0.3143942093299579 1103 88-89 0.5480778962852213 1103 90-91 0.5824587271440649 1103 92-93 0.4930987099514823 1103 94-95 0.20489974437153435 1103 96-97 0.05537941203562369 1103 98-99 -0.12353131544542961 1103 100-101 -0.33079720553609704 1104 1 0.041216545318782494 1104 2 0.057883079516635405 1104 3 0.09481660435019279 1104 4 0.013558291793138721 1104 5 0.09865646673724626 1104 6 -0.02264850499020099 1104 7 0.07278897264875894 1104 8 0.13727059386715723 1104 9 0.10562979427297137 1104 10-11 0.028912376090744374 1104 12-13 0.03911999750967965 1104 14-15 0.11223108704808027 1104 16-17 0.13990243259176083 1104 18-19 0.044977031045092986 1104 20-21 0.13869368703878138 1104 22-23 0.09151506740580118 1104 24-25 0.11182602761808624 1104 26-27 0.18945734779897805 1104 28-29 0.13275350608225978 1104 30-31 0.10472494642634445 1104 32-33 0.05607261180414724 1104 34-35 0.13680032496856143 1104 36-37 0.1692803138473309 1104 38-39 0.1722118313150247 1104 40-41 0.23700821557164886 1104 42-43 0.1596338488452318 1104 44-45 0.13700055197452343 1104 46-47 0.11745723326675517 1104 48-49 0.19768923713026965 1104 50-51 0.17629779558441783 1104 52-53 0.307358877202347 1104 54-55 0.38987218993186445 1104 56-57 0.2945131986605318 1104 58-59 0.2913412256704859 1104 60-61 0.3433881210716194 1104 62-63 0.492681815140962 1104 64-65 0.3997247360510343 1104 66-67 0.44273036637383 1104 68-69 0.31483594543813354 1104 70-71 0.24921993643403795 1104 72-73 0.39210761161771757 1104 74-75 0.32911362280318457 1104 76-77 0.2640184535474184 1104 78-79 0.37026475686966265 1104 80-81 0.48926122930510374 1104 82-83 0.5474945889611753 1104 84-85 0.42934383609722815 1104 86-87 0.41471063049744217 1104 88-89 0.5262467451668371 1104 90-91 0.590420538382471 1104 92-93 0.5792866532138952 1104 94-95 0.5253498262045966 1104 96-97 0.31744078683103893 1104 98-99 0.30002487658948596 1104 100-101 0.31609341093962584 1105 1 0.10929470703418076 1105 2 0.05868264915454802 1105 3 0.04861849889696401 1105 4 0.04326571398435419 1105 5 0.07172494219613412 1105 6 0.07880510722163336 1105 7 0.06083315731886785 1105 8 0.005216495943194843 1105 9 0.08202725314654913 1105 10-11 0.0805967689350382 1105 12-13 0.09334872998345389 1105 14-15 0.08749926005115327 1105 16-17 0.058762905927871145 1105 18-19 0.07672369786377686 1105 20-21 0.059834395332686086 1105 22-23 0.07107250550756561 1105 24-25 0.14596425137941793 1105 26-27 0.20387256014515032 1105 28-29 0.120846821684232 1105 30-31 0.10666012469181396 1105 32-33 0.06954853194669397 1105 34-35 0.1845943564932071 1105 36-37 0.053906426419352726 1105 38-39 0.18876864439670982 1105 40-41 0.33148269599131197 1105 42-43 0.2010381642823944 1105 44-45 0.08548397132153696 1105 46-47 0.05847985111621057 1105 48-49 0.1439790225607709 1105 50-51 0.19175382702820798 1105 52-53 0.11032699703206816 1105 54-55 0.2221570166624076 1105 56-57 0.21075024607809922 1105 58-59 0.08428207472346827 1105 60-61 0.14660091086896188 1105 62-63 0.20963022455294578 1105 64-65 0.114580312878239 1105 66-67 0.14071672372983102 1105 68-69 0.21695754138492873 1105 70-71 0.04894800605485017 1105 72-73 0.16506289936017282 1105 74-75 0.05618461078061188 1105 76-77 0.22366832828427263 1105 78-79 0.24740213379616094 1105 80-81 0.21688794616686025 1105 82-83 0.3725999768830519 1105 84-85 0.36675932104751396 1105 86-87 0.397520660670466 1105 88-89 0.4821778540644104 1105 90-91 0.4527914271651774 1105 92-93 0.3181551802913596 1105 94-95 0.4647509159991472 1105 96-97 0.3959972081090868 1105 98-99 0.468306662177536 1105 100-101 0.43187843305372553 1106 1 0.1165799624314019 1106 2 0.10616906088778677 1106 3 0.1156832718984191 1106 4 0.05664123664406162 1106 5 0.055805543279895176 1106 6 0.06033632156338342 1106 7 0.07635717890265425 1106 8 0.024252271195685182 1106 9 0.0718015633420066 1106 10-11 0.12067246332509285 1106 12-13 0.20267951694769692 1106 14-15 0.15561023807966734 1106 16-17 0.12518275532629985 1106 18-19 0.1287887326132875 1106 20-21 0.15918933396752522 1106 22-23 0.18835236456508397 1106 24-25 0.14042513106544874 1106 26-27 0.1366530015429035 1106 28-29 0.1956505795204393 1106 30-31 0.1950802921239827 1106 32-33 0.09759808380646007 1106 34-35 0.18248590699031553 1106 36-37 0.09475663922249566 1106 38-39 0.1681771410142474 1106 40-41 0.20855665575926707 1106 42-43 0.09815031565070598 1106 44-45 0.17420219697670802 1106 46-47 0.16165237536332455 1106 48-49 0.24597181090971532 1106 50-51 0.1615070674888699 1106 52-53 0.21601151544121677 1106 54-55 0.19036771479761683 1106 56-57 0.19227181707027796 1106 58-59 0.2201276449426004 1106 60-61 0.3352587770279385 1106 62-63 0.346547092188338 1106 64-65 0.3011100492290595 1106 66-67 0.3619311284537403 1106 68-69 0.2817097801180566 1106 70-71 0.16148294351674508 1106 72-73 0.19330876226479887 1106 74-75 0.2655247065292414 1106 76-77 0.26198834264302207 1106 78-79 0.3724595510867239 1106 80-81 0.3489424007842743 1106 82-83 0.4910306417900365 1106 84-85 0.5120004657957651 1106 86-87 0.5464058107386975 1106 88-89 0.6645656733724401 1106 90-91 0.6370238814842359 1106 92-93 0.6614263461644505 1106 94-95 0.8673640078892468 1106 96-97 0.8525021590834747 1106 98-99 0.97463348670912 1106 100-101 0.8276675759016463 1107 1 0.10225283250687767 1107 2 0.07622510942357508 1107 3 0.13917782218507568 1107 4 0.07556117768134385 1107 5 0.06879969321986579 1107 6 0.1062190694977545 1107 7 0.12072700304247519 1107 8 0.07637090207604302 1107 9 0.18778770351899254 1107 10-11 0.14279965756915658 1107 12-13 0.13026439374559828 1107 14-15 0.1907709567609075 1107 16-17 0.13974547206286303 1107 18-19 0.15356590125213643 1107 20-21 0.21046016354809893 1107 22-23 0.15577003885736218 1107 24-25 0.09616399939996256 1107 26-27 0.12381531217986463 1107 28-29 0.24929868563069846 1107 30-31 0.18214796250237697 1107 32-33 0.1731554805061677 1107 34-35 0.30958654820518205 1107 36-37 0.27311467081828766 1107 38-39 0.20602664272644944 1107 40-41 0.2268018075090481 1107 42-43 0.15843950836505627 1107 44-45 0.29864456450155785 1107 46-47 0.32349127283968215 1107 48-49 0.26368642078153925 1107 50-51 0.2659406358745997 1107 52-53 0.24486524130606568 1107 54-55 0.3261563977654873 1107 56-57 0.2429327899174325 1107 58-59 0.23850662282780632 1107 60-61 0.24693140566936478 1107 62-63 0.27430898918087365 1107 64-65 0.2020240091151706 1107 66-67 0.15532173006776162 1107 68-69 0.25595885894344406 1107 70-71 0.2898828959844124 1107 72-73 0.3636068578594802 1107 74-75 0.1980431370947855 1107 76-77 0.1772418813964869 1107 78-79 0.1893093838276343 1107 80-81 0.2686042799521964 1107 82-83 0.4702456699274755 1107 84-85 0.45196105357448957 1107 86-87 0.495385610442753 1107 88-89 0.6153573601193152 1107 90-91 0.6864475991723893 1107 92-93 0.5677281990764165 1107 94-95 0.7231926123533263 1107 96-97 0.7499097610492775 1107 98-99 0.8426125894609946 1107 100-101 0.57448074084731 1108 1 -0.023302093560666037 1108 2 0.020043000768275476 1108 3 -0.010876736816065602 1108 4 0.04729424116470682 1108 5 0.05715020793257963 1108 6 0.020895622971949024 1108 7 -0.0010596175238077876 1108 8 -0.011846630764104304 1108 9 0.018543404889065584 1108 10-11 0.024367595821843224 1108 12-13 0.05910406314902872 1108 14-15 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84-85 -0.6471070602301943 2316 86-87 -0.8323168688837121 2316 88-89 -1.2375689085506991 2316 90-91 -1.3853542508968921 2316 92-93 -1.1777790349288537 2316 94-95 -0.929473559354097 2316 96-97 -0.9880825914186104 2316 98-99 -0.7689375577350894 2316 100-101 -0.11736999112702051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 8.0 4 20.0 5 60.0 6 122.0 7 341.0 8 804.0 9 1497.0 10 2546.0 11 4014.0 12 5840.0 13 7849.0 14 9826.0 15 12875.0 16 16792.0 17 23072.0 18 21851.0 19 15694.0 20 15214.0 21 16475.0 22 17569.0 23 20113.0 24 23322.0 25 27627.0 26 33383.0 27 40359.0 28 50019.0 29 60625.0 30 74354.0 31 91700.0 32 114515.0 33 147120.0 34 202486.0 35 294569.0 36 472557.0 37 791071.0 38 634374.0 39 42362.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37498755060849 19.47395495924366 14.564889578846604 23.586167911301246 2 15.413749144406042 20.78599856545666 39.724916292999104 24.0753359971382 3 19.119359229209294 24.493491412478583 29.83330873595596 26.553840622356162 4 13.06937614370213 16.82123537139307 36.803719609332916 33.30566887557189 5 13.936266676870023 37.37914640419746 34.15303334250421 14.531553576428301 6 32.359064619436054 36.057265123894865 17.357002691135285 14.226667565533791 7 28.773617278169617 32.555782904154654 20.789703355628884 17.880896462046856 8 26.094051868277095 35.45696764746432 19.94698505997812 18.501995424280466 9 26.368783316540306 17.137417955599606 20.33125075538242 36.16254797247766 10-11 24.888491889242566 26.72731286710067 27.495920175740668 20.888275067916094 12-13 25.791428208758244 23.94206983590209 27.919890896966145 22.346611058373526 14-15 22.54423150535236 25.423790835461972 26.084452517494377 25.94752514169129 16-17 22.29372215693241 28.791789274857955 26.67442552558298 22.240063042626655 18-19 22.237507026201698 27.310386692381577 28.934240218206263 21.51786606321046 20-21 22.800603152078473 26.61527737267173 28.790232471063536 21.79388700418626 22-23 22.458711328215863 26.558167938745846 28.722706780434095 22.260413952604193 24-25 22.494195322611308 26.67444368931508 28.762988781773892 22.06837220629972 26-27 22.190910324919308 26.994703666802 28.61879914352513 22.195586864753558 28-29 22.10116870946367 27.106507251367585 28.809207557529014 21.983116481639726 30-31 22.24860113803866 26.84071892364306 28.615905575223334 22.294774363094945 32-33 21.989478395910883 26.931440732163715 28.526702123330644 22.552378748594762 34-35 22.734622389615478 26.890633173169263 28.275732221067408 22.099012216147848 36-37 22.708187719066746 26.609270793109886 28.273536516831328 22.409004970992044 38-39 22.282207228922978 26.94215905027479 28.521881199318848 22.25375252148338 40-41 22.563213402762823 26.607146273550054 28.59358207409625 22.23605824959087 42-43 22.426971423851498 26.779062782984404 28.205790054496987 22.58817573866711 44-45 22.385513140305584 26.936624316200618 28.186321400741814 22.491541142751984 46-47 22.574277359841048 27.092321385569335 27.716746496918187 22.616654757671434 48-49 22.626897915318487 27.171645731988953 27.608129434947426 22.593326917745134 50-51 22.544641975953475 27.193213672623383 27.678040274725458 22.584104076697685 52-53 22.90796964379039 27.13380035972916 27.504962750145534 22.453267246334914 54-55 22.33839674901626 26.99647194653927 27.727256991576766 22.93787431286771 56-57 22.48098927151429 27.435621949408578 27.504935455688095 22.578453323389034 58-59 22.585858610398148 26.953524050614458 27.668987164178745 22.791630174808652 60-61 22.4678919058116 27.05459765315514 27.845088561644232 22.63242187938902 62-63 22.37783356956694 27.121702946935127 27.852207601129365 22.64825588236857 64-65 22.131429557058933 26.968625285982473 27.808519438305225 23.091425718653376 66-67 22.11905291885298 27.294844281379145 27.5363700769779 23.04973272278997 68-69 22.27671688557153 26.965641790437424 27.757628873273866 23.00001245071718 70-71 22.198055045893845 27.061973851469496 27.58208691437744 23.157884188259217 72-73 22.295108401553097 26.910350758601336 27.594370419497643 23.200170420347924 74-75 22.167051848729656 27.20493259782058 27.321147702352683 23.30686785109708 76-77 22.221674186928748 26.94885917319742 27.584488774410403 23.244977865463426 78-79 22.191461966638627 27.101013352444404 27.444359753782987 23.263164927133978 80-81 22.14657766056059 27.389693238678937 27.331934926241995 23.131794174518472 82-83 22.260368401905357 27.232060160933656 27.21510011739933 23.292471319761653 84-85 22.16003539592971 27.116027347424897 27.171523282176864 23.552413974468532 86-87 22.208300621456 27.04703898293105 27.332724822747856 23.41193557286509 88-89 22.087492987121298 26.962725854321416 27.210060808002506 23.739720350554773 90-91 21.92165585253024 27.11241088500919 27.33946359406532 23.626469668395245 92-93 22.05572429558881 26.86677392337887 27.361105290744035 23.71639649028829 94-95 21.801688048164703 26.71357173961763 27.426576828541705 24.058163383675964 96-97 21.93512122862989 26.54072202109058 27.430220884448808 24.09393586583072 98-99 21.67269834111871 26.674577308301693 27.14127479762195 24.511449552957647 100-101 21.690433867893542 26.141001029906146 27.908877523767327 24.259687578432978 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 479.0 1 329.5 2 226.0 3 288.5 4 256.0 5 259.0 6 500.0 7 1027.0 8 1622.5 9 1809.5 10 1640.5 11 1609.0 12 1759.5 13 1914.0 14 2124.0 15 2352.0 16 2515.5 17 2715.5 18 2999.0 19 3333.5 20 3775.0 21 4476.0 22 5444.0 23 6613.5 24 8172.0 25 10476.5 26 13901.0 27 18297.0 28 22834.0 29 27931.0 30 34383.0 31 41908.5 32 51309.5 33 58620.5 34 64289.0 35 70971.0 36 78763.5 37 87491.5 38 93385.0 39 96294.5 40 98371.5 41 104745.5 42 111819.5 43 116446.5 44 120493.0 45 125169.0 46 126893.0 47 128144.5 48 138632.0 49 141617.0 50 135072.0 51 129794.0 52 122067.5 53 118083.0 54 114500.0 55 102787.0 56 89313.0 57 78999.0 58 69710.0 59 59434.5 60 49959.5 61 41914.5 62 34707.5 63 26685.0 64 19188.0 65 14504.0 66 10842.0 67 8771.0 68 6634.5 69 4249.0 70 3191.5 71 2630.0 72 2192.0 73 1857.5 74 1543.0 75 1322.5 76 891.0 77 431.5 78 235.0 79 149.5 80 83.0 81 42.0 82 28.0 83 17.0 84 8.0 85 2.5 86 1.5 87 2.0 88 1.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29580623825930735 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014424387965626835 16-17 7.288111814211454E-4 18-19 9.110139767764317E-5 20-21 1.5183566279607196E-5 22-23 0.0 24-25 0.0 26-27 3.036713255921439E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0017157429895956128 36-37 0.006999624054898917 38-39 0.0024749213035759727 40-41 0.0030670803884806537 42-43 2.4293706047371513E-4 44-45 0.001093216772131718 46-47 1.5183566279607195E-4 48-49 3.9477272326978705E-4 50-51 3.036713255921439E-5 52-53 3.036713255921439E-5 54-55 0.0 56-57 7.591783139803597E-4 58-59 0.0018827622186712923 60-61 5.162412535066447E-4 62-63 0.0011084003384113253 64-65 0.0 66-67 3.340384581513583E-4 68-69 0.001548723760519934 70-71 0.0 72-73 0.0 74-75 7.591783139803597E-5 76-77 0.2603677945627042 78-79 1.2146853023685756E-4 80-81 4.5550698838821585E-5 82-83 0.0 84-85 0.0 86-87 0.10875988526082635 88-89 0.37845038951920934 90-91 0.2798331265331606 92-93 0.0 94-95 0.0 96-97 0.0 98-99 4.5550698838821585E-5 100-101 4.706905546678231E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3293034.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.63509617351426 #Duplication Level Percentage of deduplicated Percentage of total 1 68.01453493768035 30.358353081402083 2 15.663052113559178 13.982436749189594 3 6.491940629404283 8.693051830386146 4 3.239016899540412 5.78295323274497 5 1.8520435271336708 4.133307047557298 6 1.1133158963413194 2.981577726281822 7 0.7490211136657549 2.340284061112461 8 0.5281739909150074 1.8860077504672161 9 0.39700881494239604 1.5948473973018094 >10 1.7360462184152332 13.575599869657248 >50 0.12290198679275688 3.801839128689556 >100 0.0846853529804781 7.1968573554638064 >500 0.005937943147743303 1.8564118284127602 >1k 0.002184071041382267 1.4126058064715694 >5k 1.365044400863917E-4 0.4038671348615152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7643 0.2320959941500756 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5614 0.17048108218742958 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4021 0.12210624002060107 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.036713255921439E-5 0.0 14-15 0.0 0.0 0.0 7.591783139803597E-5 6.073426511842878E-5 16-17 0.0 0.0 0.0 9.110139767764317E-5 9.110139767764317E-5 18-19 0.0 0.0 0.0 1.3665209651646476E-4 9.110139767764317E-5 20-21 0.0 0.0 0.0 2.2775349419410792E-4 1.0628496395725037E-4 22-23 0.0 0.0 0.0 3.947727232697871E-4 1.8220279535528634E-4 24-25 0.0 0.0 0.0 6.680769163027165E-4 2.4293706047371513E-4 26-27 0.0 0.0 0.0 0.001336153832605433 2.4293706047371513E-4 28-29 0.0 0.0 0.0 0.003811075136181406 2.4293706047371513E-4 30-31 0.0 0.0 0.0 0.011949466662050863 2.4293706047371513E-4 32-33 0.0 0.0 0.0 0.02854510460566153 2.4293706047371513E-4 34-35 0.0 0.0 0.0 0.054934142799618835 2.733041930329295E-4 36-37 3.036713255921439E-5 0.0 0.0 0.09696225426157154 2.733041930329295E-4 38-39 3.036713255921439E-5 0.0 0.0 0.1585164319590991 3.036713255921439E-4 40-41 3.036713255921439E-5 0.0 0.0 0.2272979872057197 3.036713255921439E-4 42-43 3.036713255921439E-5 0.0 0.0 0.2991162557082617 3.188548918717511E-4 44-45 3.036713255921439E-5 0.0 0.0 0.39109830023012215 3.340384581513583E-4 46-47 3.036713255921439E-5 0.0 0.0 0.503547792096893 3.340384581513583E-4 48-49 3.036713255921439E-5 0.0 0.0 0.6229817244522833 3.340384581513583E-4 50-51 3.036713255921439E-5 0.0 0.0 0.755185036048823 3.340384581513583E-4 52-53 3.036713255921439E-5 0.0 0.0 0.8920345189269228 3.644055907105727E-4 54-55 3.036713255921439E-5 0.0 0.0 1.0424125593601523 3.9477272326978705E-4 56-57 3.036713255921439E-5 0.0 0.0 1.2223530033397774 3.9477272326978705E-4 58-59 3.036713255921439E-5 0.0 0.0 1.4196786307095524 3.9477272326978705E-4 60-61 3.036713255921439E-5 0.0 0.0 1.6370161984358498 3.9477272326978705E-4 62-63 3.036713255921439E-5 0.0 0.0 1.8608978832286578 3.9477272326978705E-4 64-65 3.036713255921439E-5 0.0 0.0 2.101056351073205 3.9477272326978705E-4 66-67 3.036713255921439E-5 0.0 0.0 2.380433970617977 4.099562895493943E-4 68-69 3.036713255921439E-5 0.0 0.0 2.69458195694305 4.2513985582900147E-4 70-71 3.036713255921439E-5 0.0 0.0 3.0397499691773606 4.5550698838821584E-4 72-73 3.036713255921439E-5 0.0 0.0 3.399023514485426 5.162412535066447E-4 74-75 3.036713255921439E-5 0.0 0.0 3.7742246208207995 5.162412535066447E-4 76-77 3.036713255921439E-5 0.0 0.0 4.180385018800292 5.162412535066447E-4 78-79 3.036713255921439E-5 0.0 0.0 4.636408248442015 5.162412535066447E-4 80-81 3.036713255921439E-5 0.0 0.0 5.13172654761536 5.162412535066447E-4 82-83 3.036713255921439E-5 0.0 0.0 5.6490762014604154 5.46608386065859E-4 84-85 3.036713255921439E-5 0.0 0.0 6.191736860293577 5.46608386065859E-4 86-87 3.036713255921439E-5 0.0 0.0 6.760862475152094 5.46608386065859E-4 88-89 3.036713255921439E-5 0.0 0.0 7.3699664200248165 5.46608386065859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12805 0.0 32.525932 1 GGTATCA 8245 0.0 32.040817 1 ATCAACG 15255 0.0 26.907772 3 TCAACGC 15480 0.0 26.516668 4 CAACGCA 15725 0.0 26.163885 5 TATCAAC 15875 0.0 25.976454 2 AACGCAG 16155 0.0 25.467478 6 GTACATG 12525 0.0 24.930292 1 TACATGG 12565 0.0 24.397415 2 ACATGGG 12700 0.0 23.09184 3 ACGCAGA 18460 0.0 22.23608 7 CGCAGAG 18645 0.0 21.888191 8 CATGGGG 5770 0.0 21.136404 4 GAGTACT 8900 0.0 21.06285 12-13 GCAGAGT 20000 0.0 20.05058 9 AGTACTT 9320 0.0 19.960903 12-13 TAAGGTG 1410 0.0 19.520128 5 GTACTTT 9700 0.0 19.05676 14-15 AGAGTAC 16430 0.0 18.905092 10-11 CATGGGA 7325 0.0 17.750751 4 >>END_MODULE