FastQCFastQC Report
Fri 27 May 2016
SRR939264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353768
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75820.22607407548763062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.15227648424100892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.1079979294930359No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129000.032.7330131
GGTATCA82550.027.8485241
ATCAACG151000.027.6986333
TCAACGC152100.027.4671344
CAACGCA154700.027.0055055
TATCAAC157100.026.6533132
AACGCAG158950.026.32626
GTACATG125700.025.770511
TACATGG124800.025.1921712
ACATGGG127600.023.5616263
ACGCAGA177800.023.3489237
CGCAGAG181000.022.9623078
GCAGAGT195150.020.8359479
GAGTACT83100.020.64531312-13
CATGGGG60600.019.5629394
AGTACTT88100.019.50051312-13
AGAGTAC155750.019.27597210-11
GTACTTT90550.019.02579514-15
CATGGGA72200.018.3902454
GAGTACA107850.016.9996931
Produced by FastQC (version 0.11.5)