##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939264_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3353768 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.92233094239077 33.0 31.0 34.0 30.0 34.0 2 31.997292001116357 33.0 31.0 34.0 30.0 34.0 3 32.0593583098175 34.0 31.0 34.0 30.0 34.0 4 35.55510041243163 37.0 35.0 37.0 33.0 37.0 5 35.598758769241044 37.0 35.0 37.0 33.0 37.0 6 35.690069796121854 37.0 35.0 37.0 33.0 37.0 7 35.60998554461728 37.0 35.0 37.0 33.0 37.0 8 35.588259235582186 37.0 35.0 37.0 33.0 37.0 9 37.23757159111781 39.0 37.0 39.0 34.0 39.0 10-11 37.14876371889767 39.0 37.0 39.0 33.0 39.0 12-13 37.053220735602466 39.0 37.0 39.0 33.0 39.0 14-15 38.31577765665365 40.0 38.0 41.0 33.0 41.0 16-17 38.25843886637358 40.0 38.0 41.0 33.0 41.0 18-19 38.193995977062215 40.0 38.0 41.0 33.0 41.0 20-21 38.09148366851852 40.0 38.0 41.0 33.0 41.0 22-23 38.02207755575222 40.0 38.0 41.0 33.0 41.0 24-25 38.04270778419974 40.0 38.0 41.0 33.0 41.0 26-27 37.977654238456566 40.0 38.0 41.0 33.0 41.0 28-29 37.85516156752644 40.0 38.0 41.0 33.0 41.0 30-31 37.697449853418604 40.0 37.5 41.0 32.0 41.0 32-33 37.526522257949864 40.0 37.0 41.0 32.0 41.0 34-35 37.244171630237986 40.0 37.0 41.0 31.0 41.0 36-37 37.00717223135291 40.0 36.5 41.0 30.5 41.0 38-39 36.71093289696843 39.5 36.0 41.0 30.0 41.0 40-41 36.59621059059542 39.0 36.0 41.0 30.0 41.0 42-43 36.445534395939134 39.0 36.0 41.0 30.0 41.0 44-45 36.450640592909224 39.5 36.0 41.0 30.0 41.0 46-47 36.311608912721454 40.0 36.0 41.0 29.5 41.0 48-49 36.152564518475934 39.5 36.0 41.0 29.0 41.0 50-51 35.019030087948835 38.0 34.0 40.0 26.5 40.5 52-53 35.133210764727906 38.5 34.5 39.5 27.5 40.5 54-55 35.64631960231 39.0 35.0 40.5 28.0 41.0 56-57 35.55723443005002 39.0 35.0 41.0 27.5 41.0 58-59 35.365962254992 39.0 35.0 41.0 27.0 41.0 60-61 35.08566737472598 38.0 35.0 40.0 26.5 41.0 62-63 34.73773021866748 38.0 34.0 40.0 26.0 41.0 64-65 34.37329773556191 37.0 34.0 40.0 26.0 41.0 66-67 34.02780633603756 37.0 34.0 40.0 25.5 41.0 68-69 33.62405524174601 36.0 33.5 39.0 24.5 41.0 70-71 33.21151135081496 36.0 33.0 39.0 23.5 40.5 72-73 32.777512487446955 35.0 33.0 38.5 22.0 40.0 74-75 32.33123415215364 35.0 32.0 37.5 21.5 39.5 76-77 31.88792829438411 35.0 32.0 37.0 20.0 39.0 78-79 31.460561374549464 35.0 31.5 36.5 19.0 39.0 80-81 31.00151978312155 35.0 31.0 36.0 17.5 37.5 82-83 30.565749181219452 34.5 31.0 35.5 13.0 37.0 84-85 30.148642661030816 34.0 30.5 35.0 7.5 37.0 86-87 29.819388669699276 34.0 30.0 35.0 4.5 36.0 88-89 29.41255015254484 34.0 30.0 35.0 2.0 36.0 90-91 29.0196912845492 34.0 29.0 35.0 2.0 35.5 92-93 28.62126122021559 34.0 29.0 35.0 2.0 35.0 94-95 28.15817119729212 34.0 29.0 35.0 2.0 35.0 96-97 27.59928966463989 34.0 28.0 35.0 2.0 35.0 98-99 26.880153308159656 34.0 26.0 35.0 2.0 35.0 100-101 24.36004473177632 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3679829394218359 1101 2 0.1554395818013674 1101 3 0.3206454211044374 1101 4 0.329893572814413 1101 5 0.3056064730244543 1101 6 0.35540675062717497 1101 7 0.30399867725010665 1101 8 0.2808171519872502 1101 9 0.3988728359697902 1101 10-11 0.3535517846772436 1101 12-13 0.3806497224330201 1101 14-15 0.3564623577492583 1101 16-17 0.4144561212754283 1101 18-19 0.4771478911777649 1101 20-21 0.5465590560041562 1101 22-23 0.5017597974409469 1101 24-25 0.49553750420464837 1101 26-27 0.44276598779965326 1101 28-29 0.38900151490238244 1101 30-31 0.46441060401600964 1101 32-33 0.5039079961080759 1101 34-35 0.4493807000401162 1101 36-37 0.40679495130304133 1101 38-39 0.6853139713011416 1101 40-41 0.5448436871215989 1101 42-43 0.37903546480526273 1101 44-45 0.5531983843337542 1101 46-47 0.6176568806657414 1101 48-49 0.5278380234439126 1101 50-51 0.691707151632599 1101 52-53 0.639091857745882 1101 54-55 0.7031740581787105 1101 56-57 0.6885171509104495 1101 58-59 0.5673505668851888 1101 60-61 0.7222766136684058 1101 62-63 0.7227643167332403 1101 64-65 0.7118758382713679 1101 66-67 0.7102334170937823 1101 68-69 0.7500549250884987 1101 70-71 0.6188807159633569 1101 72-73 0.6705914926841459 1101 74-75 0.7012610898058185 1101 76-77 0.648452912124089 1101 78-79 0.5930482987567487 1101 80-81 0.6246584095155292 1101 82-83 0.46470837362946327 1101 84-85 0.5936705800518212 1101 86-87 0.5135099375076599 1101 88-89 0.4021583452102142 1101 90-91 0.5755589731394721 1101 92-93 0.6411351914770371 1101 94-95 0.6750928205536404 1101 96-97 0.5577612359700517 1101 98-99 0.6409226573721902 1101 100-101 0.35730994990560916 1102 1 0.3251258287290071 1102 2 0.3011521595907567 1102 3 0.35626552971688596 1102 4 0.30974229441171985 1102 5 0.2511065090484834 1102 6 0.26816803740545936 1102 7 0.33118072017273903 1102 8 0.36280919067705497 1102 9 0.3534065724720392 1102 10-11 0.39612261692234796 1102 12-13 0.37900803993079535 1102 14-15 0.46081614297400364 1102 16-17 0.5033155217455345 1102 18-19 0.4983077297901275 1102 20-21 0.5243103911446951 1102 22-23 0.5280110695394384 1102 24-25 0.49465514066817207 1102 26-27 0.5300656604312977 1102 28-29 0.51711432864915 1102 30-31 0.541850894189551 1102 32-33 0.4977660681921634 1102 34-35 0.492478327407575 1102 36-37 0.4673615867488863 1102 38-39 0.6735611321574311 1102 40-41 0.5796776276099038 1102 42-43 0.527982606298643 1102 44-45 0.5827292501712691 1102 46-47 0.7078669570366785 1102 48-49 0.6470862244940108 1102 50-51 0.6329669906952589 1102 52-53 0.7125017346337614 1102 54-55 0.6690837324314316 1102 56-57 0.7434349936115296 1102 58-59 0.5757438839776867 1102 60-61 0.6820396318515378 1102 62-63 0.6448276267109989 1102 64-65 0.6799752105526835 1102 66-67 0.5628560789092134 1102 68-69 0.566835986806673 1102 70-71 0.5327273391610348 1102 72-73 0.5526422392821289 1102 74-75 0.40820899906129426 1102 76-77 0.4769712213363313 1102 78-79 0.5431393157150595 1102 80-81 0.4200705243961522 1102 82-83 0.28401134245967796 1102 84-85 0.36085719352300316 1102 86-87 0.2885444417720038 1102 88-89 0.12761973420667516 1102 90-91 0.08239385700700907 1102 92-93 0.221167973342542 1102 94-95 0.1890640643732695 1102 96-97 -0.03842590210104291 1102 98-99 -0.033943434561130914 1102 100-101 -0.11598352434171133 1103 1 0.25236546540027405 1103 2 0.262173910336557 1103 3 0.3026456000673008 1103 4 0.30420164255722426 1103 5 0.35758442436083016 1103 6 0.18891584452064336 1103 7 0.2223206863332976 1103 8 0.3218612410800077 1103 9 0.3383329939511768 1103 10-11 0.37224967066472914 1103 12-13 0.36553515600898123 1103 14-15 0.4990093505967792 1103 16-17 0.4757466740675511 1103 18-19 0.4393970481035012 1103 20-21 0.3958707900938947 1103 22-23 0.43004421883370725 1103 24-25 0.41042721422442696 1103 26-27 0.3947944439924598 1103 28-29 0.4153182780680922 1103 30-31 0.48542084387564444 1103 32-33 0.531831197984225 1103 34-35 0.47447134802290947 1103 36-37 0.4348061524018476 1103 38-39 0.625807447665693 1103 40-41 0.5933695217280786 1103 42-43 0.4797744826065795 1103 44-45 0.6131655451977664 1103 46-47 0.5719885230013304 1103 48-49 0.5236017639214054 1103 50-51 0.4634322273487186 1103 52-53 0.599874836730919 1103 54-55 0.7683273541421087 1103 56-57 0.7153710771415192 1103 58-59 0.6072332089698946 1103 60-61 0.6822914796631068 1103 62-63 0.5666671453894807 1103 64-65 0.5421485041098251 1103 66-67 0.42217583936159286 1103 68-69 0.4444026082056638 1103 70-71 0.4930678485336628 1103 72-73 0.45266978948472314 1103 74-75 0.39896882808306344 1103 76-77 0.5149287216702767 1103 78-79 0.5042375948013955 1103 80-81 0.4453376778758269 1103 82-83 0.31110487957174726 1103 84-85 0.3406737481336286 1103 86-87 0.41468346176398896 1103 88-89 0.2753336239613411 1103 90-91 0.18832577739527423 1103 92-93 0.22377271976936797 1103 94-95 0.25692216134998347 1103 96-97 0.06884150740071604 1103 98-99 -0.13416856702473012 1103 100-101 -0.18307856419210822 1104 1 0.24176032036349682 1104 2 0.26094945688804927 1104 3 0.27692190037325304 1104 4 0.27150226867168925 1104 5 0.3116116570536249 1104 6 0.20442929866671733 1104 7 0.28328818478020423 1104 8 0.28861356648143044 1104 9 0.3243686059340689 1104 10-11 0.3250962333959748 1104 12-13 0.3024797259024794 1104 14-15 0.3091028225106811 1104 16-17 0.4306884303684484 1104 18-19 0.33386114034353653 1104 20-21 0.43042364725795323 1104 22-23 0.510009139037507 1104 24-25 0.49140546241848426 1104 26-27 0.3767596758753129 1104 28-29 0.4060943801292112 1104 30-31 0.41654523138389266 1104 32-33 0.42036098453706927 1104 34-35 0.4473608885401319 1104 36-37 0.6114896433769417 1104 38-39 0.6734002569965511 1104 40-41 0.49620755038983333 1104 42-43 0.5043098263758594 1104 44-45 0.6132713994634216 1104 46-47 0.5374599428796714 1104 48-49 0.48883332874111574 1104 50-51 0.5667909932692581 1104 52-53 0.6194032790353106 1104 54-55 0.5134048392525372 1104 56-57 0.504165366841896 1104 58-59 0.4720196240034298 1104 60-61 0.5487304297923288 1104 62-63 0.5292951299285065 1104 64-65 0.5366676172450866 1104 66-67 0.4492299527830852 1104 68-69 0.5251130138336961 1104 70-71 0.4189403378740266 1104 72-73 0.4654237708136648 1104 74-75 0.4775461933984886 1104 76-77 0.5021481840132971 1104 78-79 0.4412972951230394 1104 80-81 0.41405379337941994 1104 82-83 0.43080188300021405 1104 84-85 0.5592484419142529 1104 86-87 0.5346753941529556 1104 88-89 0.4932165810535203 1104 90-91 0.5493500989632523 1104 92-93 0.566920869095437 1104 94-95 0.5248748751840786 1104 96-97 0.5117108417856997 1104 98-99 0.39846486659716973 1104 100-101 0.2737687279105394 1105 1 0.274093996139797 1105 2 0.22401308798446706 1105 3 0.3018723597499431 1105 4 0.3674767785806239 1105 5 0.3197446774817294 1105 6 0.19633766352590243 1105 7 0.30324157358604964 1105 8 0.34133071346087007 1105 9 0.3341310706145677 1105 10-11 0.37063993057901 1105 12-13 0.4161697760679388 1105 14-15 0.40893204733990984 1105 16-17 0.48849901072001245 1105 18-19 0.4824783442886087 1105 20-21 0.4725108614763158 1105 22-23 0.49786255721879513 1105 24-25 0.3589891783628616 1105 26-27 0.345842431843316 1105 28-29 0.35702136999062617 1105 30-31 0.49077177282354967 1105 32-33 0.4126890854717331 1105 34-35 0.4208513804732448 1105 36-37 0.42542666633035253 1105 38-39 0.6110845635333249 1105 40-41 0.5958152422106409 1105 42-43 0.41618533456284723 1105 44-45 0.5317427892462803 1105 46-47 0.4891872511643527 1105 48-49 0.4885831911958931 1105 50-51 0.4122836891717867 1105 52-53 0.41275436746836647 1105 54-55 0.5167921438693455 1105 56-57 0.46443589678455766 1105 58-59 0.46338937152071935 1105 60-61 0.4377123679546031 1105 62-63 0.35539922447521377 1105 64-65 0.29628685637251806 1105 66-67 0.3657943734458655 1105 68-69 0.2796246187551361 1105 70-71 0.20885928762904626 1105 72-73 0.4467709755215239 1105 74-75 0.4486217320969743 1105 76-77 0.4793718723025542 1105 78-79 0.36486211827802606 1105 80-81 0.46376279800646003 1105 82-83 0.41395259360994174 1105 84-85 0.3600588226426673 1105 86-87 0.3966838627188025 1105 88-89 0.46980792649732805 1105 90-91 0.3944733703160317 1105 92-93 0.4061761530228516 1105 94-95 0.32748044418309163 1105 96-97 0.32240216272292344 1105 98-99 0.2324800428254612 1105 100-101 0.03127670877917765 1106 1 0.12104847551372444 1106 2 0.18654746869295735 1106 3 0.20309684378958082 1106 4 0.2526632325912743 1106 5 0.1747124425682074 1106 6 0.14952094203422206 1106 7 0.2684321957862821 1106 8 0.21430033699578388 1106 9 0.19048718756989302 1106 10-11 0.2510270903613119 1106 12-13 0.3266664742271601 1106 14-15 0.3722269071864943 1106 16-17 0.356214325721929 1106 18-19 0.4431120168357907 1106 20-21 0.5002268248197481 1106 22-23 0.42657040473670094 1106 24-25 0.4059086355423531 1106 26-27 0.31766856506006746 1106 28-29 0.3517407654307618 1106 30-31 0.2592592508220477 1106 32-33 0.22007695666683702 1106 34-35 0.2802171438101837 1106 36-37 0.22257671567091108 1106 38-39 0.4789354720145269 1106 40-41 0.5485363570289792 1106 42-43 0.28605770726166924 1106 44-45 0.437379374433462 1106 46-47 0.24441751621655072 1106 48-49 0.3259500020646797 1106 50-51 0.2842775685144545 1106 52-53 0.20411791978443006 1106 54-55 0.39555546278247533 1106 56-57 0.40159358323563055 1106 58-59 0.3452278075564408 1106 60-61 0.42335875269651524 1106 62-63 0.4089605095938822 1106 64-65 0.3638987893038461 1106 66-67 0.26800131034755026 1106 68-69 0.31437210050657427 1106 70-71 0.2739474385707794 1106 72-73 0.3045430625191585 1106 74-75 0.38673493879412746 1106 76-77 0.35991235212229 1106 78-79 0.29708658194680737 1106 80-81 0.33130848649618727 1106 82-83 0.3270817835904616 1106 84-85 0.313886084482931 1106 86-87 0.3510502665623143 1106 88-89 0.4441330147285747 1106 90-91 0.6002643136705714 1106 92-93 0.5384404182011941 1106 94-95 0.5235545893810389 1106 96-97 0.503999928902374 1106 98-99 0.4196116291232421 1106 100-101 0.5257103246797215 1107 1 0.12705838438496642 1107 2 0.09297114422888342 1107 3 0.08847205234894062 1107 4 0.03284567895126855 1107 5 0.20694422366278786 1107 6 5.285894468585184E-4 1107 7 0.10457984117824282 1107 8 0.06632927611203598 1107 9 0.24164591363690846 1107 10-11 0.21764257913380192 1107 12-13 0.3555986502979991 1107 14-15 0.29745203798755426 1107 16-17 0.23539625369329542 1107 18-19 0.19618212394111367 1107 20-21 0.2864129952685772 1107 22-23 0.30857358494237985 1107 24-25 0.1767281711454558 1107 26-27 0.16364869255156833 1107 28-29 0.20310689416299255 1107 30-31 0.2258123252254549 1107 32-33 0.1850930418223271 1107 34-35 0.28525486154048707 1107 36-37 0.28768217329287893 1107 38-39 0.3999432893906487 1107 40-41 0.3449486166840856 1107 42-43 0.3834624592518949 1107 44-45 0.34408070420889203 1107 46-47 0.3723287782804192 1107 48-49 0.4551070492701541 1107 50-51 0.30799214883211334 1107 52-53 0.39267814965060666 1107 54-55 0.4142810954479401 1107 56-57 0.4047828536296052 1107 58-59 0.2844090651162716 1107 60-61 0.22485518676815275 1107 62-63 0.3629871806418308 1107 64-65 0.4411187248876871 1107 66-67 0.1312317874444986 1107 68-69 0.19471582333012094 1107 70-71 0.17263225167232576 1107 72-73 0.32649266645702113 1107 74-75 0.3411897761910083 1107 76-77 0.23064430931793467 1107 78-79 0.3370926345353986 1107 80-81 0.2698630981248975 1107 82-83 0.3224329517589446 1107 84-85 0.2528846312951245 1107 86-87 0.2194442815814206 1107 88-89 0.3478237718309245 1107 90-91 0.4515777065100117 1107 92-93 0.37231805959901365 1107 94-95 0.2731908203085389 1107 96-97 0.4881847320893762 1107 98-99 0.5500667724543966 1107 100-101 0.4900082196819433 1108 1 0.09602798651235389 1108 2 0.1640843649734549 1108 3 0.1667037356996488 1108 4 0.15513754339607289 1108 5 0.1519560196752181 1108 6 0.0732411798548398 1108 7 0.11515293496178458 1108 8 0.1652601114448089 1108 9 0.22634538662371995 1108 10-11 0.18321737369532798 1108 12-13 0.1745314098353532 1108 14-15 0.23556086406772891 1108 16-17 0.3052239051514505 1108 18-19 0.2345868306731873 1108 20-21 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-0.9863498353762559 2316 86-87 -0.7973861418299037 2316 88-89 -1.0949344101157799 2316 90-91 -0.9996557705522662 2316 92-93 -1.073970906259472 2316 94-95 -1.107207850787809 2316 96-97 -1.1805824977119919 2316 98-99 -1.2293246179463964 2316 100-101 -0.6641622758294723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8443.0 3 2166.0 4 2250.0 5 2490.0 6 2973.0 7 3658.0 8 4698.0 9 5959.0 10 7294.0 11 9469.0 12 11981.0 13 16716.0 14 15684.0 15 16402.0 16 19464.0 17 25046.0 18 21036.0 19 16876.0 20 17319.0 21 18623.0 22 20553.0 23 23042.0 24 26533.0 25 30462.0 26 35550.0 27 41593.0 28 50102.0 29 59636.0 30 72061.0 31 88167.0 32 110064.0 33 142079.0 34 191558.0 35 276393.0 36 432724.0 37 703138.0 38 706070.0 39 115474.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36193901872701 18.790344018776352 14.114407391647706 23.733309570848935 2 15.001011917945691 20.286298153413927 40.33979547914464 24.372894449495742 3 19.076501184122105 24.41390209804406 29.592395108992076 26.91720160884176 4 12.81748460305282 16.28744749514134 37.08178965814747 33.81327824365837 5 13.970058083556127 37.28217920068309 34.15826612150823 14.589496594252548 6 32.38873257421185 35.670793831598424 17.684376153278617 14.256097440911114 7 28.820070923149682 32.420353183560266 20.931519029453842 17.828056863836206 8 26.089472039950216 35.10488692538068 20.242103903148887 18.563537131520206 9 26.515656268937594 17.118105523279493 20.49930940730766 35.86692880047526 10-11 24.85436901894068 26.525380845453334 27.685177505100516 20.93507263050547 12-13 25.787050394916378 23.832221035014793 28.00467496491837 22.37605360515046 14-15 22.632737851042407 25.288076679222765 26.148644314325143 25.930541155409692 16-17 22.377148019702204 28.597168908205013 26.758825511780998 22.266857560311784 18-19 22.307764148159464 27.212472358921218 28.90537001524418 21.574393477675137 20-21 22.856998777045575 26.575305391726612 28.747202027386002 21.820493803841813 22-23 22.53226706264129 26.580915600796345 28.56851264668322 22.31830468987915 24-25 22.52901990458584 26.619971794462778 28.67104348400908 22.179964816942302 26-27 22.21692158471984 26.919585167499815 28.53041856300828 22.33307468477207 28-29 22.118884031093668 27.10412487841213 28.700035198747585 22.076955891746618 30-31 22.238100922085284 26.82802661812539 28.51763600011936 22.416236459669957 32-33 21.979628861549664 26.899869006448224 28.465227030623847 22.655275101378265 34-35 22.777328271631763 26.876400887481555 28.161593258344354 22.18467758254233 36-37 22.717159385062544 26.56986325229605 28.189117554064552 22.52385980857686 38-39 22.3341133148256 26.911919535350037 28.422277252753798 22.331689897070568 40-41 22.59013119115128 26.59825695684929 28.512346817395972 22.299265034603454 42-43 22.428327596289655 26.739316105696048 28.14864062060306 22.683715677411236 44-45 22.401808044180676 26.9629750436338 28.06689344524135 22.568323466944168 46-47 22.53823467358564 27.089167104339772 27.699216078838017 22.673382143236566 48-49 22.59652601953693 27.166067507569487 27.561956961406654 22.67544951148693 50-51 22.517992415173484 27.211163677108573 27.658220726113065 22.612623181604878 52-53 22.86952261612576 27.08450861450548 27.52691312830344 22.519055641065314 54-55 22.298622123614035 26.941056969868892 27.821663209744436 22.938657696772637 56-57 22.467854088179763 27.420886847527214 27.590153797183014 22.52110526711001 58-59 22.61942294588465 26.885006751182576 27.686578816541925 22.80899148639085 60-61 22.47715926666872 26.97632851787059 27.958247584968245 22.588264630492443 62-63 22.41580811434053 27.016220363076897 27.94475244580072 22.62321907678185 64-65 22.171766464314885 26.84894788147172 27.916435088954273 23.062850565259126 66-67 22.15332867713269 27.197035555584154 27.648882564224664 23.000753203058487 68-69 22.34781727581066 26.897437474752145 27.830165592992103 22.924579656445093 70-71 22.2491598900089 27.007822467232266 27.663700962152692 23.079316680606134 72-73 22.3752843289836 26.864659403807018 27.705240632108225 23.05481563510116 74-75 22.186939320242693 27.171241596491203 27.52453952520578 23.11727955806033 76-77 22.3876458797554 26.883112452575425 27.670035689255094 23.059205978414077 78-79 22.335754674163162 27.02759993453733 27.592290244955443 23.04435514634407 80-81 22.322563196039773 27.298334613088386 27.46631087830689 22.912791312564952 82-83 22.44775337844156 27.1545237267025 27.375935546853086 23.02178734800286 84-85 22.35593453650975 27.0122182954236 27.35771506204907 23.27413210601758 86-87 22.496340804928657 26.964022366161284 27.515800406562267 23.023836422347788 88-89 22.43921833515318 26.83423881530128 27.42084807699991 23.305694772545632 90-91 22.312573751853712 26.971849916511854 27.579705884642074 23.135870446992364 92-93 22.534916995432205 26.71266889768258 27.592283837906116 23.160130268979103 94-95 22.32760465697536 26.56029478013467 27.652549767345796 23.459550795544175 96-97 22.57147745034487 26.389859476536742 27.646678390051655 23.39198468306673 98-99 22.347998126306685 26.467998305893904 27.405054482272494 23.778949085526914 100-101 22.545420594699436 25.975252103059532 27.985567991032212 23.493759311208823 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2407.0 1 1351.5 2 352.5 3 399.5 4 337.0 5 346.5 6 626.5 7 1188.5 8 1881.5 9 2205.0 10 2096.5 11 2108.5 12 2348.5 13 2645.0 14 2952.0 15 3153.0 16 3344.0 17 3552.5 18 3712.5 19 3932.5 20 4210.0 21 4863.0 22 5821.5 23 6941.5 24 8462.5 25 10690.0 26 14093.0 27 18567.5 28 22998.5 29 28047.5 30 34874.0 31 42585.5 32 51688.0 33 58823.5 34 65205.5 35 72538.0 36 80365.5 37 88978.0 38 94736.0 39 97888.5 40 100038.5 41 106489.0 42 113747.5 43 117939.0 44 121881.0 45 127225.0 46 129194.5 47 130151.0 48 140048.0 49 142531.0 50 135728.0 51 130555.5 52 123694.0 53 119479.0 54 114990.5 55 103803.0 56 91021.0 57 80608.0 58 70892.0 59 60509.0 60 51102.5 61 42977.5 62 35578.0 63 27301.5 64 19761.0 65 15108.5 66 11556.5 67 9397.0 68 7027.0 69 4631.5 70 3601.0 71 2960.0 72 2468.5 73 2101.5 74 1684.5 75 1389.0 76 972.0 77 519.0 78 316.5 79 205.5 80 116.0 81 80.0 82 73.0 83 53.0 84 32.5 85 22.0 86 13.0 87 12.0 88 12.5 89 12.5 90 9.5 91 5.5 92 5.0 93 5.5 94 4.5 95 1.5 96 2.5 97 3.5 98 3.0 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2850823312763435 2 0.11014476851111943 3 0.1201335333869248 4 0.121594576607565 5 0.12770710436738617 6 0.05698068560496731 7 0.06717817094086413 8 0.07147184897703121 9 0.06854976253575083 10-11 0.06398772962232331 12-13 0.06854976253575083 14-15 0.06389827799656983 16-17 0.06450953077255195 18-19 0.06932500995894766 20-21 0.07327579009639307 22-23 0.08084936107685445 24-25 0.08967525481786456 26-27 0.08998833550800174 28-29 0.08338382380653642 30-31 0.07955231250342898 32-33 0.07329069870068532 34-35 0.24214555091467269 36-37 0.36742255278242264 38-39 0.3393496509001219 40-41 0.32266692269709774 42-43 0.34586471097583377 44-45 0.3588650139186729 46-47 0.36475391261411044 48-49 0.37442959679977866 50-51 0.36854069810434115 52-53 0.3688090529816016 54-55 0.35549566934862514 56-57 0.33153754225098453 58-59 0.31755327142485706 60-61 0.2741692329344188 62-63 0.34620760887455543 64-65 0.3744146881954864 66-67 0.3787829092531147 68-69 0.39172357777878497 70-71 0.3916192175487392 72-73 0.3747426774899158 74-75 0.3780374790385023 76-77 0.3621598154672595 78-79 0.3400652639061497 80-81 0.33787369907518944 82-83 0.3520070559442394 84-85 0.35953590111182404 86-87 0.35316992707903466 88-89 0.3685854239172179 90-91 0.392811905892119 92-93 0.38726590509540315 94-95 0.38681864696663576 96-97 0.39214101869896784 98-99 0.380855205249737 100-101 0.358686110667166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3353768.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.67815587923081 #Duplication Level Percentage of deduplicated Percentage of total 1 68.88567066385478 31.465704024289153 2 15.319778975449443 13.99558504151885 3 6.287661349043078 8.61626325652213 4 3.185797150708624 5.820853553987116 5 1.7803802377069997 4.066224301114117 6 1.0913442443237533 2.9910355506073056 7 0.7149645814125878 2.2860784518525237 8 0.5024484516394643 1.8360734956212488 9 0.36220543281410844 1.4890388598348399 >10 1.6660803613405364 13.285483754301197 >50 0.11719858413816689 3.7208651540337616 >100 0.07850330110127621 6.854463827534947 >500 0.005485245765904352 1.7104318581772728 >1k 0.00235081961161231 1.4834103047525733 >5k 1.306010895340172E-4 0.3784885658530806 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7582 0.22607407548763062 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5107 0.15227648424100892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3622 0.1079979294930359 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.981720858449362E-5 0.0 12-13 0.0 0.0 0.0 2.981720858449362E-5 0.0 14-15 0.0 0.0 0.0 2.981720858449362E-5 0.0 16-17 0.0 0.0 0.0 4.472581287674043E-5 0.0 18-19 0.0 0.0 0.0 8.945162575348086E-5 2.981720858449362E-5 20-21 0.0 0.0 0.0 1.6399464721471492E-4 2.981720858449362E-5 22-23 0.0 0.0 0.0 3.578065030139235E-4 4.472581287674043E-5 24-25 0.0 0.0 0.0 6.26161380274366E-4 5.963441716898724E-5 26-27 0.0 0.0 0.0 0.0010436023004572767 5.963441716898724E-5 28-29 0.0 0.0 0.0 0.00345879619580126 5.963441716898724E-5 30-31 0.0 0.0 0.0 0.009988764875805363 5.963441716898724E-5 32-33 0.0 0.0 0.0 0.02528499287965059 5.963441716898724E-5 34-35 0.0 0.0 0.0 0.047394453045052604 5.963441716898724E-5 36-37 0.0 0.0 0.0 0.08673825977229194 5.963441716898724E-5 38-39 0.0 0.0 0.0 0.14668575763141636 5.963441716898724E-5 40-41 0.0 0.0 0.0 0.2155187836487199 5.963441716898724E-5 42-43 0.0 0.0 0.0 0.2865881003098604 7.454302146123404E-5 44-45 0.0 0.0 0.0 0.37739640905393573 8.945162575348086E-5 46-47 0.0 0.0 0.0 0.4872579140835025 8.945162575348086E-5 48-49 0.0 0.0 0.0 0.6016069090050356 8.945162575348086E-5 50-51 0.0 0.0 0.0 0.7292394703509605 8.945162575348086E-5 52-53 0.0 0.0 0.0 0.8596003062823665 1.1926883433797448E-4 54-55 0.0 0.0 0.0 1.0056748111378009 1.490860429224681E-4 56-57 0.0 0.0 0.0 1.1784059004677725 1.490860429224681E-4 58-59 0.0 0.0 0.0 1.3720090358068895 1.490860429224681E-4 60-61 0.0 0.0 0.0 1.5882583410659294 1.490860429224681E-4 62-63 0.0 0.0 0.0 1.8104263622289913 1.490860429224681E-4 64-65 0.0 0.0 0.0 2.0479353372087754 1.490860429224681E-4 66-67 0.0 0.0 0.0 2.319853370895065 1.490860429224681E-4 68-69 0.0 0.0 0.0 2.6268662590853036 1.490860429224681E-4 70-71 0.0 0.0 0.0 2.9683925662120934 2.3853766867594896E-4 72-73 0.0 0.0 0.0 3.318536046619802 2.981720858449362E-4 74-75 0.0 0.0 0.0 3.689864653726793 2.981720858449362E-4 76-77 0.0 0.0 0.0 4.1002090782665945 3.1308069013718306E-4 78-79 0.0 0.0 0.0 4.5511049064813065 3.2798929442942983E-4 80-81 0.0 0.0 0.0 5.047009811054313 3.5780650301392344E-4 82-83 0.0 0.0 0.0 5.5529482063160005 4.472581287674043E-4 84-85 0.0 0.0 0.0 6.088196917616246 4.472581287674043E-4 86-87 0.0 0.0 0.0 6.661611655904642 4.472581287674043E-4 88-89 0.0 0.0 2.981720858449362E-5 7.269062737792238 4.472581287674043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12900 0.0 32.733013 1 GGTATCA 8255 0.0 27.848524 1 ATCAACG 15100 0.0 27.698633 3 TCAACGC 15210 0.0 27.467134 4 CAACGCA 15470 0.0 27.005505 5 TATCAAC 15710 0.0 26.653313 2 AACGCAG 15895 0.0 26.3262 6 GTACATG 12570 0.0 25.77051 1 TACATGG 12480 0.0 25.192171 2 ACATGGG 12760 0.0 23.561626 3 ACGCAGA 17780 0.0 23.348923 7 CGCAGAG 18100 0.0 22.962307 8 GCAGAGT 19515 0.0 20.835947 9 GAGTACT 8310 0.0 20.645313 12-13 CATGGGG 6060 0.0 19.562939 4 AGTACTT 8810 0.0 19.500513 12-13 AGAGTAC 15575 0.0 19.275972 10-11 GTACTTT 9055 0.0 19.025795 14-15 CATGGGA 7220 0.0 18.390245 4 GAGTACA 10785 0.0 16.999693 1 >>END_MODULE