FastQCFastQC Report
Fri 27 May 2016
SRR939264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3353768
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79250.23630137803211196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55930.16676764761307283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40540.12087896360153713No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131650.031.623771
GGTATCA89100.028.6228961
ATCAACG159100.025.8863033
TCAACGC161500.025.5603754
GTACATG126000.025.3761181
CAACGCA163700.025.2168645
TATCAAC164800.025.0197542
AACGCAG169500.024.3539856
TACATGG126100.024.3073672
ACATGGG127500.022.9612463
ACGCAGA192250.021.4226827
CATGGGG58450.021.3496974
CGCAGAG193850.021.1479578
AGTACTT98450.019.95434612-13
GAGTACT93900.019.88529412-13
GCAGAGT210500.019.2512369
AGAGTAC169050.018.89090310-11
GTACTTT99350.018.41235514-15
GTAGGAC8450.016.8455433
GAGTACA111200.016.8156741
Produced by FastQC (version 0.11.5)