##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939264_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3353768 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.310528635254435 34.0 31.0 34.0 31.0 34.0 2 32.51657419356378 34.0 31.0 34.0 31.0 34.0 3 32.610498996949104 34.0 31.0 34.0 31.0 34.0 4 36.13370573039042 37.0 35.0 37.0 35.0 37.0 5 36.066524875900775 37.0 35.0 37.0 35.0 37.0 6 36.09874087891589 37.0 35.0 37.0 35.0 37.0 7 36.044643219208965 37.0 35.0 37.0 35.0 37.0 8 36.02239928343284 37.0 35.0 37.0 35.0 37.0 9 37.73948197967182 39.0 38.0 39.0 35.0 39.0 10-11 37.66522445798278 39.0 37.0 39.0 35.0 39.0 12-13 37.591627089291805 39.0 37.0 39.0 35.0 39.0 14-15 38.92197343405984 40.0 38.0 41.0 35.0 41.0 16-17 38.871308927749325 40.0 38.0 41.0 35.0 41.0 18-19 38.96574345631541 40.0 38.0 41.0 35.0 41.0 20-21 38.963712457152674 40.0 38.0 41.0 35.0 41.0 22-23 38.90949105603011 40.0 38.0 41.0 35.0 41.0 24-25 38.84918634801215 40.0 38.0 41.0 35.0 41.0 26-27 38.699036725259475 40.0 38.0 41.0 34.0 41.0 28-29 38.56286212999826 40.0 38.0 41.0 34.0 41.0 30-31 38.391941392487496 40.0 38.0 41.0 34.0 41.0 32-33 38.22168050980271 40.0 38.0 41.0 33.5 41.0 34-35 38.178848238757126 40.0 38.0 41.0 33.5 41.0 36-37 38.10664139558848 40.0 38.0 41.0 33.0 41.0 38-39 38.012888339324604 40.0 38.0 41.0 33.0 41.0 40-41 37.855969166620945 40.0 37.5 41.0 33.0 41.0 42-43 37.701181626159 40.0 37.0 41.0 33.0 41.0 44-45 37.511020738464914 40.0 37.0 41.0 32.5 41.0 46-47 37.26641332972346 40.0 37.0 41.0 32.0 41.0 48-49 36.99911234170044 40.0 36.5 41.0 31.0 41.0 50-51 36.781417199997136 40.0 36.0 41.0 31.0 41.0 52-53 36.5689163651153 39.0 36.0 41.0 30.5 41.0 54-55 36.30172972608719 39.0 35.0 41.0 30.0 41.0 56-57 36.07153655231966 39.0 35.0 40.0 29.5 41.0 58-59 35.78800113782468 38.5 35.0 40.0 29.0 41.0 60-61 35.46777654268274 38.0 35.0 40.0 28.0 41.0 62-63 35.10437573499419 38.0 34.0 40.0 28.0 41.0 64-65 34.696357350896065 37.0 34.0 40.0 27.5 41.0 66-67 34.333593886041015 36.5 34.0 39.0 26.5 41.0 68-69 33.957897206962436 36.0 33.5 39.0 26.0 40.0 70-71 33.57084106592943 35.5 33.0 39.0 26.0 40.0 72-73 33.090227320434806 35.0 33.0 38.0 25.5 40.0 74-75 32.59066697517538 35.0 32.0 37.0 24.5 39.0 76-77 30.56926224473488 33.5 29.5 35.5 20.5 37.5 78-79 31.560807724326786 34.5 31.0 36.0 22.5 38.0 80-81 31.602109478055727 35.0 32.0 36.0 23.0 37.5 82-83 31.30236304359753 34.5 31.0 35.5 22.5 37.0 84-85 30.93793205135239 34.0 31.0 35.0 20.5 36.5 86-87 30.427019549354636 34.0 31.0 35.0 19.0 36.0 88-89 29.94468937624785 34.0 30.0 35.0 15.0 36.0 90-91 29.547276376899056 34.0 30.0 35.0 8.5 35.0 92-93 29.22371463977234 34.0 29.0 35.0 4.5 35.0 94-95 28.858830425956718 34.0 29.0 35.0 2.0 35.0 96-97 28.34614156375754 34.0 29.0 35.0 2.0 35.0 98-99 27.636399566099982 33.5 29.0 35.0 2.0 35.0 100-101 24.30918194102872 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22846329215742855 1101 2 0.21717785793430977 1101 3 0.23878670836431581 1101 4 0.12143301296596576 1101 5 0.19206181816186785 1101 6 0.1413653699477493 1101 7 0.21784168008283444 1101 8 0.215580724994048 1101 9 0.2749559229793306 1101 10-11 0.273038817563517 1101 12-13 0.26396348995536556 1101 14-15 0.3017159486297416 1101 16-17 0.30043193831629367 1101 18-19 0.29405543394842226 1101 20-21 0.30525326704855615 1101 22-23 0.32494425412844663 1101 24-25 0.2918963486871604 1101 26-27 0.35738592720594653 1101 28-29 0.37191983462768263 1101 30-31 0.3259073459736612 1101 32-33 0.29860613050801277 1101 34-35 0.31590355965871453 1101 36-37 0.45641899549371345 1101 38-39 0.3679148411414488 1101 40-41 0.41667587209665413 1101 42-43 0.42265126382663 1101 44-45 0.5170603886615197 1101 46-47 0.4848875741604459 1101 48-49 0.681140317485216 1101 50-51 0.57717911945673 1101 52-53 0.6262421546933652 1101 54-55 0.589086772517426 1101 56-57 0.5322276280059484 1101 58-59 0.5359020143096487 1101 60-61 0.6777453662373887 1101 62-63 0.6763789849406905 1101 64-65 0.6409256126952059 1101 66-67 0.5862882163264658 1101 68-69 0.6326834485064055 1101 70-71 0.6050327295382019 1101 72-73 0.48787633162062605 1101 74-75 0.5072962372740619 1101 76-77 0.6262434275221977 1101 78-79 0.7898101162290452 1101 80-81 0.7384248461155067 1101 82-83 0.7488012817651573 1101 84-85 0.7944196772698895 1101 86-87 0.8579702633722661 1101 88-89 0.9626632728904703 1101 90-91 1.178263282126423 1101 92-93 0.8981000778356147 1101 94-95 0.9751280499709942 1101 96-97 0.9890172831777093 1101 98-99 0.9821951516557768 1101 100-101 0.7151831523954435 1102 1 0.14290037168947123 1102 2 0.20359488559175531 1102 3 0.21464779520923827 1102 4 0.10786343705883183 1102 5 0.13804862886460967 1102 6 0.12054888535252672 1102 7 0.18883772194275394 1102 8 0.18430972974021387 1102 9 0.31391253203756975 1102 10-11 0.2501207249457451 1102 12-13 0.23197539139362533 1102 14-15 0.30157033275395406 1102 16-17 0.27966155321943376 1102 18-19 0.26858909164810285 1102 20-21 0.1990109221545069 1102 22-23 0.16605825216513637 1102 24-25 0.2744198543654477 1102 26-27 0.3133853913485325 1102 28-29 0.2216622628272873 1102 30-31 0.21200775619727352 1102 32-33 0.23524127239765136 1102 34-35 0.27460291212681653 1102 36-37 0.29883711963251613 1102 38-39 0.2606452485732049 1102 40-41 0.458539901654369 1102 42-43 0.36215397463473664 1102 44-45 0.3877100932644879 1102 46-47 0.32905967445133655 1102 48-49 0.30997580700672245 1102 50-51 0.4154142347786518 1102 52-53 0.497140312514631 1102 54-55 0.46731047272547244 1102 56-57 0.4267568164746578 1102 58-59 0.42030642455127776 1102 60-61 0.39181196115620054 1102 62-63 0.3130849928479549 1102 64-65 0.4011067910411654 1102 66-67 0.3538917516345137 1102 68-69 0.4166219667480746 1102 70-71 0.3067281103572057 1102 72-73 0.5306821643610675 1102 74-75 0.5007957577041822 1102 76-77 0.48557297533058374 1102 78-79 0.5507329955395441 1102 80-81 0.6361171131075878 1102 82-83 0.527810193209092 1102 84-85 0.4011799238543574 1102 86-87 0.4616738711786752 1102 88-89 0.5479294454275525 1102 90-91 0.6271628043577522 1102 92-93 0.6588552202656146 1102 94-95 0.6212395938707758 1102 96-97 0.5102538979997959 1102 98-99 0.6923932162648683 1102 100-101 0.4583581751356114 1103 1 0.15207041487928308 1103 2 0.12144908658022757 1103 3 0.19052327942492298 1103 4 0.13758314853307496 1103 5 0.06941856964558468 1103 6 0.14215269674217268 1103 7 0.11825521604311717 1103 8 0.047654602619687125 1103 9 0.1750917920224353 1103 10-11 0.16250301044375703 1103 12-13 0.11974588429179533 1103 14-15 0.16305041868832149 1103 16-17 0.1636443106886034 1103 18-19 0.10834347741772632 1103 20-21 0.21415935930664887 1103 22-23 0.16485494836710757 1103 24-25 0.16039262697331225 1103 26-27 0.24941159465232232 1103 28-29 0.19447716999145115 1103 30-31 0.1387000065200823 1103 32-33 0.16413865206727252 1103 34-35 0.24208588109248552 1103 36-37 0.30423946390430245 1103 38-39 0.3337854707558492 1103 40-41 0.3726191683180673 1103 42-43 0.37970874109807085 1103 44-45 0.3595067560722711 1103 46-47 0.21413191720479574 1103 48-49 0.1500557860797045 1103 50-51 0.19384660042270951 1103 52-53 0.1867822711948932 1103 54-55 0.07984553661967908 1103 56-57 0.1754516604124987 1103 58-59 0.0068068252814939 1103 60-61 -0.012479748180815875 1103 62-63 0.17921104476479144 1103 64-65 0.2527982065093539 1103 66-67 0.20610884723093648 1103 68-69 0.07880333056659339 1103 70-71 0.22362779422559242 1103 72-73 0.19600926040273947 1103 74-75 0.14476727246441357 1103 76-77 0.3500315442784441 1103 78-79 0.3796097821284441 1103 80-81 0.45015185419134696 1103 82-83 0.34807434121211855 1103 84-85 0.4250473044144911 1103 86-87 0.3251601127744692 1103 88-89 0.49638218152444225 1103 90-91 0.6600630336644855 1103 92-93 0.5391223180883173 1103 94-95 0.4338843020056018 1103 96-97 0.17103799346264026 1103 98-99 0.1403634063890955 1103 100-101 0.3184287481798229 1104 1 0.18878196757106736 1104 2 0.12536129485816616 1104 3 0.15649142455286835 1104 4 0.10332675752215437 1104 5 0.08909431741030005 1104 6 0.06540088020452117 1104 7 0.1328692534742899 1104 8 0.15565786358835254 1104 9 0.18987778300282088 1104 10-11 0.17226519084020708 1104 12-13 0.1840185059367343 1104 14-15 0.12241170984533056 1104 16-17 0.16451515682303608 1104 18-19 0.1379021547111705 1104 20-21 0.22099451288809746 1104 22-23 0.15508656619344663 1104 24-25 0.18357365101924472 1104 26-27 0.1311942716574137 1104 28-29 0.2324730736380971 1104 30-31 0.12860202904154505 1104 32-33 0.20254339594977466 1104 34-35 0.28557459809849917 1104 36-37 0.30339793044333163 1104 38-39 0.26581738499534424 1104 40-41 0.33279183215630326 1104 42-43 0.4465329965137599 1104 44-45 0.432489694294091 1104 46-47 0.37431385720551447 1104 48-49 0.3633783166592366 1104 50-51 0.23058476244917614 1104 52-53 0.2345357436600608 1104 54-55 0.26402861944361433 1104 56-57 0.3363932386110804 1104 58-59 0.3435850603299144 1104 60-61 0.34797347981771765 1104 62-63 0.3812317109946761 1104 64-65 0.2783751308095006 1104 66-67 0.30447894597171654 1104 68-69 0.34447750085360695 1104 70-71 0.26808589671500016 1104 72-73 0.28691607684497455 1104 74-75 0.31858050673893246 1104 76-77 0.38743912719673546 1104 78-79 0.4122597008162536 1104 80-81 0.41821975146022794 1104 82-83 0.4408571687560645 1104 84-85 0.3639051490795815 1104 86-87 0.3414196884244909 1104 88-89 0.7054905779886873 1104 90-91 0.7657326241775735 1104 92-93 0.7102146153750155 1104 94-95 0.7154641755953577 1104 96-97 0.6910132287591253 1104 98-99 0.689642251013904 1104 100-101 0.6384740052514424 1105 1 0.05891209589791657 1105 2 0.09944923615922363 1105 3 0.11512196105226025 1105 4 0.1223201699581935 1105 5 0.10808245525089433 1105 6 0.1314960241685199 1105 7 0.09410381716786986 1105 8 0.16087281552461263 1105 9 0.13041929731483037 1105 10-11 0.13642593778783407 1105 12-13 0.10369372069391858 1105 14-15 0.15370365302251798 1105 16-17 0.09001332207530055 1105 18-19 0.14841787219409497 1105 20-21 0.14174655659095947 1105 22-23 0.18162265010658274 1105 24-25 0.19740262591379576 1105 26-27 0.10562364157858184 1105 28-29 0.19501269396460685 1105 30-31 0.12205747331665862 1105 32-33 0.12833775002282266 1105 34-35 0.2069143534546498 1105 36-37 0.2419097398567729 1105 38-39 0.2620423976138042 1105 40-41 0.24285003934565452 1105 42-43 0.1787012122885301 1105 44-45 0.21758360643718078 1105 46-47 0.1926417779924563 1105 48-49 0.24752629097113754 1105 50-51 0.34563261520195 1105 52-53 0.33382979992133244 1105 54-55 0.288751459740233 1105 56-57 0.2329789118360921 1105 58-59 0.24621684001087374 1105 60-61 0.2253959392073952 1105 62-63 0.15640918084427824 1105 64-65 0.21841150117374042 1105 66-67 0.14250261186422364 1105 68-69 0.22339469944294166 1105 70-71 0.2990656244160377 1105 72-73 0.18547728893078386 1105 74-75 0.24326171830539067 1105 76-77 0.3815163651264335 1105 78-79 0.38716175563633826 1105 80-81 0.44005251919463717 1105 82-83 0.43917781871606465 1105 84-85 0.252488246679075 1105 86-87 0.38185595427059127 1105 88-89 0.5365043065433959 1105 90-91 0.5740781702280344 1105 92-93 0.5052316644904238 1105 94-95 0.4778783416980481 1105 96-97 0.39893696946975155 1105 98-99 0.6551232697736076 1105 100-101 0.8708134425089078 1106 1 0.03900164537695616 1106 2 0.06255341308477114 1106 3 0.08562868989702821 1106 4 0.07247201115016821 1106 5 0.05812862265150187 1106 6 0.12638920843422596 1106 7 0.04938059239942305 1106 8 0.05854474061315784 1106 9 0.14448879795866532 1106 10-11 0.15569504927006506 1106 12-13 0.16938996113577787 1106 14-15 0.13084378724292378 1106 16-17 0.15158412234027452 1106 18-19 0.0785281255095569 1106 20-21 0.041685277544374344 1106 22-23 0.12321109466280689 1106 24-25 0.09323392705883293 1106 26-27 0.14448588839730547 1106 28-29 0.13360696615129797 1106 30-31 0.12975589226437023 1106 32-33 0.19350664984406762 1106 34-35 0.11626181240640676 1106 36-37 0.16888410627432648 1106 38-39 0.22010664962943594 1106 40-41 0.10844864660622022 1106 42-43 0.12126006348220386 1106 44-45 0.16929793110232794 1106 46-47 0.25143851881293955 1106 48-49 0.04831263521424489 1106 50-51 0.09395182409210179 1106 52-53 0.14449003292470053 1106 54-55 0.14259006266023277 1106 56-57 0.1987784070307299 1106 58-59 0.1870701710564049 1106 60-61 0.04148965609251576 1106 62-63 0.17112320347237642 1106 64-65 0.26203798085166596 1106 66-67 0.2002706736599933 1106 68-69 0.10487336469774533 1106 70-71 0.09437955490520977 1106 72-73 0.23820973503518417 1106 74-75 0.2532984759396584 1106 76-77 0.16338440745236227 1106 78-79 0.26946357957185185 1106 80-81 0.293885441781093 1106 82-83 0.2026509822740188 1106 84-85 0.14011670546355148 1106 86-87 0.2535152815545665 1106 88-89 0.5798026979904627 1106 90-91 0.5241979860912096 1106 92-93 0.5279042261680722 1106 94-95 0.40812604929171314 1106 96-97 0.4571784087174109 1106 98-99 0.735158826702893 1106 100-101 0.6856104273878607 1107 1 0.03629148316569086 1107 2 0.05393683088050949 1107 3 0.034052420198968036 1107 4 0.0036923206536769726 1107 5 0.01777938545706803 1107 6 0.06501614896872354 1107 7 0.08939736213005745 1107 8 0.10993508914216932 1107 9 0.11652281709466195 1107 10-11 0.09019763696413463 1107 12-13 0.11838486317542873 1107 14-15 0.15947460861035978 1107 16-17 0.09206451469154331 1107 18-19 0.13496601188141 1107 20-21 0.15837026249788977 1107 22-23 0.20612848957579644 1107 24-25 0.11751420251299294 1107 26-27 0.08915861898346122 1107 28-29 0.06216994376050167 1107 30-31 0.09050268349539436 1107 32-33 0.11496638124509673 1107 34-35 0.06398515150905126 1107 36-37 0.11368298753689743 1107 38-39 0.0964247400707876 1107 40-41 0.1642282077416155 1107 42-43 0.13623493249229313 1107 44-45 0.04153156291042137 1107 46-47 0.04816445552419424 1107 48-49 0.13238554577284845 1107 50-51 0.033707794295615656 1107 52-53 0.023060894951171917 1107 54-55 0.04405613936475561 1107 56-57 0.07480269670138995 1107 58-59 0.1015302395516855 1107 60-61 -0.02083590707677274 1107 62-63 0.08376806193528097 1107 64-65 0.05651322330930242 1107 66-67 0.018909105614632438 1107 68-69 0.0506249857245038 1107 70-71 -0.01716903907185241 1107 72-73 -0.06035794489713453 1107 74-75 -0.011120470834384832 1107 76-77 0.09483733087153468 1107 78-79 0.19858120469094587 1107 80-81 0.1242994633910044 1107 82-83 0.20997050701834397 1107 84-85 0.18495651959903725 1107 86-87 0.23960556426994017 1107 88-89 0.3383056879314559 1107 90-91 0.2531049509115988 1107 92-93 0.31429194498425517 1107 94-95 0.4148740718137631 1107 96-97 0.351989706756882 1107 98-99 0.43093240134660604 1107 100-101 0.4240671211637057 1108 1 0.05321937656293585 1108 2 0.08385666176937434 1108 3 0.006005816146469556 1108 4 -0.004198377230700601 1108 5 0.05313213045801035 1108 6 0.04383202447724699 1108 7 0.06834455776147763 1108 8 0.12361518055556786 1108 9 0.09625191462448157 1108 10-11 0.11428685439652497 1108 12-13 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-0.9133563029978404 2316 98-99 -1.0346185988654533 2316 100-101 -0.3352705475999933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 27.0 5 78.0 6 141.0 7 352.0 8 788.0 9 1528.0 10 2536.0 11 3993.0 12 5693.0 13 8301.0 14 9682.0 15 13045.0 16 16419.0 17 23188.0 18 21903.0 19 15462.0 20 14886.0 21 15763.0 22 16845.0 23 18937.0 24 22238.0 25 26745.0 26 32080.0 27 39707.0 28 49028.0 29 59444.0 30 73575.0 31 90247.0 32 111739.0 33 143485.0 34 196538.0 35 281693.0 36 449401.0 37 781385.0 38 737909.0 39 68974.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3794604867448 19.45567873910649 14.569453256795942 23.595407517352772 2 15.39790468511835 20.783995792195526 39.73503832107647 24.083061201609652 3 19.110087519470635 24.577639240400647 29.77928109517414 26.53299214495457 4 13.12100300318925 16.840431419227567 36.78590767160996 33.25265790597322 5 13.943629970826843 37.35798063551206 34.18059925433125 14.517790139329852 6 32.334407150405156 36.07566176312733 17.36545282798333 14.224478258484188 7 28.783624866120732 32.56361203279416 20.79559468633489 17.857168414750216 8 26.0985554158785 35.42341032534153 19.943538133824404 18.53449612495557 9 26.370398906543326 17.112185458266644 20.412622459275656 36.104793175914374 10-11 24.88009009567746 26.725849850079076 27.494820750868875 20.899239303374593 12-13 25.786279790373097 23.93084435178581 27.91583973608192 22.367036121759167 14-15 22.53459433147195 25.42123346132658 26.079761337689728 25.964410869511738 16-17 22.286296491655452 28.82642906575839 26.66425046373504 22.223023978851117 18-19 22.25578811653042 27.31211580526739 28.923855198093605 21.50824088010858 20-21 22.77891613254107 26.61552617831645 28.791526426395624 21.81403126274686 22-23 22.494996672399523 26.587960168980086 28.65751298241262 22.259530176207775 24-25 22.494097308205564 26.735403314452572 28.720380503532372 22.05011887380949 26-27 22.215127303759203 26.976645857501076 28.649469598108734 22.158757240630994 28-29 22.097891088471236 27.136522263913303 28.797713497176908 21.967873150438553 30-31 22.221513235262545 26.85813389596418 28.603648195104732 22.316704673668543 32-33 21.988074309254547 26.95694812521319 28.50305984194494 22.55191772358732 34-35 22.754697424832557 26.899186735868962 28.27096240927039 22.075153430028095 36-37 22.725712421325877 26.61088100702878 28.292279438079472 22.371127133565878 38-39 22.280018786202575 26.97671852751955 28.516799487106848 22.22646319917102 40-41 22.578325243178092 26.598307054308197 28.611369698845817 22.211998003667897 42-43 22.449462777603422 26.75222168465608 28.185043352762744 22.613272184977752 44-45 22.40630482671924 26.934686207789017 28.128857624365338 22.530151341126405 46-47 22.534147085803756 27.161366625881637 27.744204384119513 22.560281904195094 48-49 22.667653218708033 27.17012625798803 27.62027665598813 22.54194386731581 50-51 22.510561255280628 27.291944463659977 27.621707882000184 22.575786399059208 52-53 22.917894141753393 27.12329833190608 27.48663592711243 22.47217159922809 54-55 22.347565484553495 26.999273652798884 27.739068414988754 22.91409244765887 56-57 22.48867877692774 27.482704152866887 27.50186171223855 22.526755357966824 58-59 22.57840212669305 26.94193353323882 27.66118777732936 22.81847656273877 60-61 22.478001499540284 27.057354210408853 27.877285781397205 22.587358508653665 62-63 22.374827132362192 27.10838882888922 27.814559465572874 22.702224573175712 64-65 22.154278732798264 26.952941728966007 27.759840239372586 23.132939298863146 66-67 22.134238265735735 27.27907833815577 27.534820536184974 23.051862859923524 68-69 22.284139672369587 26.97353424086511 27.737455566510132 23.00487052025517 70-71 22.175997266358316 27.106719963933106 27.569617218603078 23.147665551105504 72-73 22.349384334277147 26.950999592100587 27.529453438639763 23.170162634982503 74-75 22.168960405132378 27.253778436671826 27.316692746785108 23.260568411410688 76-77 22.231555433747268 26.99050204176663 27.57728392286985 23.200658601616258 78-79 22.17972441743138 27.082896014274095 27.5068818117413 23.230497756553227 80-81 22.160064215087147 27.37648152250823 27.356906297437867 23.106547964966758 82-83 22.27208322102185 27.22482592713628 27.218892302627967 23.2841985492139 84-85 22.16068612975018 27.13213913425139 27.170677876346844 23.536496859651592 86-87 22.215816364983624 27.079523585303406 27.33352088250592 23.37113916720705 88-89 22.118445227397903 27.002527665843562 27.191133762795626 23.687893343962905 90-91 21.908988875174487 27.147423202882585 27.36821692609231 23.575370995850616 92-93 22.045114629276682 26.845476490920063 27.394858559089357 23.714550320713894 94-95 21.825562121638146 26.71852278348496 27.42522065486362 24.030694440013274 96-97 21.94537606656155 26.556592465549194 27.45790704664127 24.04012442124798 98-99 21.69728198253427 26.685194086174118 27.16367083232949 24.45385309896212 100-101 21.608539902354433 26.317565170098533 27.839050594526388 24.23484433302065 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 445.0 1 298.5 2 209.5 3 287.0 4 262.0 5 254.5 6 476.5 7 1049.0 8 1718.0 9 1926.5 10 1757.5 11 1675.5 12 1807.0 13 2000.0 14 2201.0 15 2387.5 16 2537.5 17 2750.0 18 3027.0 19 3452.5 20 3915.0 21 4584.5 22 5520.0 23 6787.0 24 8485.5 25 10777.0 26 14329.5 27 19024.0 28 23438.5 29 28357.0 30 35000.5 31 42618.5 32 51917.5 33 59341.0 34 65556.0 35 72401.0 36 80266.0 37 89133.5 38 94914.0 39 98074.5 40 100397.5 41 106924.5 42 114148.0 43 118627.5 44 122615.0 45 127620.5 46 129185.0 47 130386.0 48 141124.0 49 144316.5 50 137518.5 51 132004.0 52 124515.5 53 120174.5 54 116009.0 55 104076.5 56 90745.0 57 80555.0 58 71338.0 59 60847.0 60 50901.0 61 42441.0 62 35233.0 63 27038.5 64 19425.5 65 14790.0 66 11091.0 67 9073.0 68 6745.5 69 4235.0 70 3218.5 71 2672.5 72 2240.0 73 1872.5 74 1562.5 75 1350.0 76 914.5 77 463.5 78 266.5 79 171.0 80 92.0 81 43.0 82 25.0 83 15.0 84 9.0 85 5.5 86 1.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27867163143067736 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022511992481292686 16-17 6.26161380274366E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.472581287674043E-5 26-27 0.0011181453219185108 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0036376994473082216 36-37 0.02075277717480756 38-39 0.007245581686031949 40-41 0.018441943509509305 42-43 0.00523292010657863 44-45 5.963441716898725E-4 46-47 1.3417743863022128E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 1.490860429224681E-5 58-59 4.919839416441447E-4 60-61 0.002191564830960281 62-63 0.0013715915948867064 64-65 1.490860429224681E-5 66-67 0.0 68-69 5.814355673976256E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23528759294023918 78-79 0.0 80-81 0.0011628711347952511 82-83 0.0 84-85 0.0 86-87 0.1054187409504772 88-89 0.36220454128013624 90-91 0.363397229623516 92-93 0.0 94-95 2.981720858449362E-5 96-97 0.0 98-99 0.0 100-101 1.7890325150696172E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3353768.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.45176758191345 #Duplication Level Percentage of deduplicated Percentage of total 1 67.85602962672866 30.163204579987752 2 15.758359144549406 14.009738363316613 3 6.377240514287474 8.504388394652072 4 3.2759852943283247 5.824933476209961 5 1.9248714055191 4.278196817160306 6 1.1458898960264219 3.056209879957769 7 0.7593200174917893 2.362718185648763 8 0.5405900982890958 1.9224148324984505 9 0.38259536171537467 1.5306336087200947 >10 1.7636124547131027 13.68164493785551 >50 0.12338658410407412 3.8202818418122764 >100 0.08347897570974541 7.063677378442014 >500 0.006028344097857836 1.8270232145359493 >1k 0.0024783192377258335 1.552442722395112 >5k 1.3396320203923424E-4 0.4024917668073864 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7925 0.23630137803211196 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5593 0.16676764761307283 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4054 0.12087896360153713 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.981720858449362E-5 9 0.0 0.0 0.0 0.0 5.963441716898724E-5 10-11 0.0 0.0 0.0 0.0 5.963441716898724E-5 12-13 0.0 0.0 0.0 0.0 5.963441716898724E-5 14-15 0.0 0.0 0.0 0.0 8.945162575348086E-5 16-17 0.0 0.0 0.0 1.490860429224681E-5 8.945162575348086E-5 18-19 0.0 0.0 0.0 5.963441716898724E-5 8.945162575348086E-5 20-21 0.0 0.0 0.0 1.341774386302213E-4 8.945162575348086E-5 22-23 0.0 0.0 0.0 2.9817208584493617E-4 1.1926883433797448E-4 24-25 0.0 0.0 0.0 5.665269631053788E-4 1.490860429224681E-4 26-27 0.0 0.0 0.0 9.839678832882894E-4 1.490860429224681E-4 28-29 0.0 0.0 0.0 0.0033693445700477788 1.490860429224681E-4 30-31 0.0 0.0 0.0 0.009884404645759634 1.490860429224681E-4 32-33 0.0 0.0 0.0 0.025419170318280813 1.490860429224681E-4 34-35 0.0 0.0 0.0 0.04791625419528125 1.490860429224681E-4 36-37 0.0 0.0 0.0 0.08778186207274921 1.490860429224681E-4 38-39 0.0 0.0 0.0 0.14810207503917983 1.490860429224681E-4 40-41 0.0 0.0 0.0 0.21723327314232826 1.490860429224681E-4 42-43 0.0 0.0 0.0 0.2888094823494052 1.490860429224681E-4 44-45 0.0 0.0 0.0 0.3803781299123851 1.6399464721471492E-4 46-47 0.0 0.0 0.0 0.49108942538660993 2.0872046009145535E-4 48-49 0.0 0.0 0.0 0.6060645816884174 2.6835487726044256E-4 50-51 0.0 0.0 0.0 0.7343531216232011 2.6835487726044256E-4 52-53 0.0 0.0 0.0 0.8652953931220049 2.832634815526894E-4 54-55 0.0 0.0 0.0 1.0115338926246538 3.2798929442942983E-4 56-57 0.0 0.0 0.0 1.1856514821538042 3.5780650301392344E-4 58-59 0.0 0.0 0.0 1.3803578542105477 3.5780650301392344E-4 60-61 0.0 0.0 0.0 1.5978147564172596 3.5780650301392344E-4 62-63 0.0 0.0 0.0 1.820668573377765 3.8762371159841704E-4 64-65 0.0 0.0 0.0 2.059265876470883 3.8762371159841704E-4 66-67 0.0 0.0 0.0 2.3326747705863973 3.8762371159841704E-4 68-69 0.0 0.0 0.0 2.6417450461689658 3.8762371159841704E-4 70-71 0.0 0.0 0.0 2.9847174879121035 3.8762371159841704E-4 72-73 0.0 0.0 0.0 3.3366350922305896 3.8762371159841704E-4 74-75 0.0 0.0 0.0 3.71008072114708 3.8762371159841704E-4 76-77 0.0 0.0 0.0 4.122750887956471 3.8762371159841704E-4 78-79 0.0 0.0 0.0 4.575733920772099 3.8762371159841704E-4 80-81 0.0 0.0 0.0 5.075023078519445 3.8762371159841704E-4 82-83 0.0 0.0 0.0 5.582899592339125 3.8762371159841704E-4 84-85 0.0 0.0 0.0 6.120295142657453 4.025323158906639E-4 86-87 0.0 0.0 0.0 6.694589488599092 4.472581287674043E-4 88-89 0.0 0.0 0.0 7.304127775087602 4.472581287674043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13165 0.0 31.62377 1 GGTATCA 8910 0.0 28.622896 1 ATCAACG 15910 0.0 25.886303 3 TCAACGC 16150 0.0 25.560375 4 GTACATG 12600 0.0 25.376118 1 CAACGCA 16370 0.0 25.216864 5 TATCAAC 16480 0.0 25.019754 2 AACGCAG 16950 0.0 24.353985 6 TACATGG 12610 0.0 24.307367 2 ACATGGG 12750 0.0 22.961246 3 ACGCAGA 19225 0.0 21.422682 7 CATGGGG 5845 0.0 21.349697 4 CGCAGAG 19385 0.0 21.147957 8 AGTACTT 9845 0.0 19.954346 12-13 GAGTACT 9390 0.0 19.885294 12-13 GCAGAGT 21050 0.0 19.251236 9 AGAGTAC 16905 0.0 18.890903 10-11 GTACTTT 9935 0.0 18.412355 14-15 GTAGGAC 845 0.0 16.845543 3 GAGTACA 11120 0.0 16.815674 1 >>END_MODULE