##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939263_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 763058 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0102023699378 33.0 31.0 34.0 30.0 34.0 2 31.953281401937993 33.0 31.0 34.0 30.0 34.0 3 31.976701639980185 34.0 31.0 34.0 30.0 34.0 4 35.482726083731514 37.0 35.0 37.0 33.0 37.0 5 35.55980410401306 37.0 35.0 37.0 33.0 37.0 6 35.74295400873852 37.0 35.0 37.0 35.0 37.0 7 35.70928553268559 37.0 35.0 37.0 35.0 37.0 8 35.730312767836786 37.0 35.0 37.0 35.0 37.0 9 37.296792642236895 39.0 37.0 39.0 34.0 39.0 10-11 37.21940468483392 39.0 37.0 39.0 34.0 39.0 12-13 37.121695860603 39.0 37.0 39.0 33.5 39.0 14-15 38.081650411895296 40.0 38.0 41.0 33.0 41.0 16-17 38.07404024333668 40.0 38.0 41.0 33.5 41.0 18-19 38.22844397149365 40.0 38.0 41.0 34.0 41.0 20-21 38.26970033732691 40.0 38.0 41.0 34.0 41.0 22-23 38.2588243619751 40.0 38.0 41.0 34.0 41.0 24-25 38.14985099428877 40.0 38.0 41.0 34.0 41.0 26-27 37.88334241958016 40.0 37.5 41.0 33.5 41.0 28-29 37.63979605744255 40.0 37.0 41.0 33.0 41.0 30-31 37.31742016989534 39.0 36.0 41.0 33.0 41.0 32-33 36.870701178678424 39.0 35.0 41.0 32.5 41.0 34-35 36.5266768712208 39.0 35.0 41.0 31.5 41.0 36-37 36.14581656964477 39.0 35.0 41.0 31.0 41.0 38-39 35.36288329327522 38.0 35.0 40.5 28.0 41.0 40-41 35.14961903289134 38.0 35.0 40.0 29.0 41.0 42-43 35.07848682537893 38.0 35.0 40.5 26.5 41.0 44-45 34.64946635773427 38.0 35.0 41.0 23.5 41.0 46-47 33.880056955041425 38.0 34.5 41.0 13.5 41.0 48-49 33.31056223773291 38.0 34.0 40.5 2.0 41.0 50-51 32.05885725593598 37.0 32.0 39.5 2.0 40.5 52-53 32.00123123012929 37.5 32.5 39.5 2.0 40.5 54-55 32.412969263149066 38.0 33.0 40.0 2.0 41.0 56-57 32.395591160829184 38.0 33.0 40.0 2.0 41.0 58-59 32.212345588408745 38.0 33.0 40.0 2.0 41.0 60-61 31.837539217202362 37.0 32.5 40.0 2.0 41.0 62-63 31.307665341297778 36.0 31.5 39.5 2.0 41.0 64-65 30.7729707309274 35.5 31.0 39.0 2.0 41.0 66-67 30.342807493008394 35.0 30.5 39.0 2.0 40.5 68-69 29.956146845980253 35.0 30.0 38.0 2.0 40.0 70-71 29.413238574263033 35.0 29.0 37.0 2.0 39.5 72-73 28.842561771189082 35.0 29.0 37.0 2.0 39.0 74-75 28.365272364617105 35.0 28.0 36.0 2.0 39.0 76-77 27.779984614537817 34.0 26.5 35.5 2.0 37.5 78-79 27.352835564269036 34.0 26.0 35.0 2.0 37.0 80-81 27.00021426942644 34.0 25.5 35.0 2.0 36.5 82-83 26.535028136786458 34.0 24.5 35.0 2.0 36.0 84-85 26.086494080397557 34.0 21.0 35.0 2.0 36.0 86-87 25.540639767881338 34.0 17.5 35.0 2.0 35.0 88-89 24.957087141475483 33.0 5.0 35.0 2.0 35.0 90-91 24.391291356620336 33.0 2.0 35.0 2.0 35.0 92-93 23.918487847581705 32.0 2.0 35.0 2.0 35.0 94-95 23.447657320937594 32.0 2.0 35.0 2.0 35.0 96-97 22.740006002164975 31.5 2.0 35.0 2.0 35.0 98-99 21.957685392198236 31.0 2.0 35.0 2.0 35.0 100-101 19.707743710176683 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44765870813969144 1101 2 0.44552375290775714 1101 3 0.47365185595119996 1101 4 0.48486876967252357 1101 5 0.3875087133814077 1101 6 0.40936925052458406 1101 7 0.42235762518740927 1101 8 0.3190869768007616 1101 9 0.5718763659532939 1101 10-11 0.7557589093144585 1101 12-13 0.7350808025763698 1101 14-15 0.87398176712545 1101 16-17 0.7938688848924258 1101 18-19 0.7816227111563734 1101 20-21 0.9090934154512667 1101 22-23 1.1158843713326974 1101 24-25 1.0801487382626718 1101 26-27 0.8295386157342648 1101 28-29 0.8509032692795273 1101 30-31 0.8939717823537521 1101 32-33 0.8713332881946485 1101 34-35 0.6752994213096457 1101 36-37 0.8931021356148037 1101 38-39 1.1075496731423158 1101 40-41 0.9139313166340344 1101 42-43 0.7171576051345525 1101 44-45 0.3568864608531044 1101 46-47 0.44764691547258906 1101 48-49 0.5691124088335329 1101 50-51 0.5182324431618781 1101 52-53 0.6307642610356865 1101 54-55 0.8140289360616251 1101 56-57 1.075524076994725 1101 58-59 1.0892213034285163 1101 60-61 0.9641211625961468 1101 62-63 1.209810310659691 1101 64-65 0.9608460707027859 1101 66-67 0.9815269467787111 1101 68-69 0.9273084537570888 1101 70-71 1.1041933892613507 1101 72-73 1.0322951714067798 1101 74-75 0.983545777594081 1101 76-77 0.9318211989269294 1101 78-79 1.0367440742705938 1101 80-81 0.9718520466577196 1101 82-83 0.7613096203685501 1101 84-85 0.7335137975614252 1101 86-87 0.7204273012127835 1101 88-89 0.692062623765711 1101 90-91 0.7004033352452375 1101 92-93 0.6435338275853617 1101 94-95 0.2855209486196877 1101 96-97 0.3131381557307229 1101 98-99 0.3114164194694631 1101 100-101 -0.0357906681085467 1102 1 0.3830394131282375 1102 2 0.22378334734382221 1102 3 0.35642376813487786 1102 4 0.2527937459308376 1102 5 0.21067501693986657 1102 6 0.2635184600659528 1102 7 0.45345225677223056 1102 8 0.21715831359758653 1102 9 0.3101381949013984 1102 10-11 0.23310305209977855 1102 12-13 0.23141131483826172 1102 14-15 0.5033146256249736 1102 16-17 0.45556725369863926 1102 18-19 0.2098250462214537 1102 20-21 0.24393155298547953 1102 22-23 0.29310212453084006 1102 24-25 0.3614249239506151 1102 26-27 0.3107687146114273 1102 28-29 0.22597309778073082 1102 30-31 0.33016948080635444 1102 32-33 0.22908564839799794 1102 34-35 0.3437327490566986 1102 36-37 0.4924227042981215 1102 38-39 0.9530715713602973 1102 40-41 0.6062983631677454 1102 42-43 0.606887927462914 1102 44-45 0.7727082026552381 1102 46-47 0.9806304080149104 1102 48-49 1.11152624046305 1102 50-51 0.8790328174426421 1102 52-53 0.9912259670856258 1102 54-55 0.9756714420668757 1102 56-57 0.7672487013173921 1102 58-59 0.9085575136960955 1102 60-61 1.111214900376158 1102 62-63 0.9840931719523596 1102 64-65 0.7696870572398495 1102 66-67 0.7757004566385 1102 68-69 0.8974183638179802 1102 70-71 1.1211617150532085 1102 72-73 1.0430347948915006 1102 74-75 0.8767605421323736 1102 76-77 0.9873313659567025 1102 78-79 0.8879185896607957 1102 80-81 0.5971662749131248 1102 82-83 0.7959620140895716 1102 84-85 0.641287804600136 1102 86-87 0.6495339153684192 1102 88-89 0.771132927100723 1102 90-91 0.7621003074814219 1102 92-93 0.49306039034722104 1102 94-95 0.8018363779253157 1102 96-97 0.8713856000075779 1102 98-99 0.6978543279661977 1102 100-101 0.630031073693587 1103 1 0.35420239070147375 1103 2 0.35163235019282624 1103 3 0.4542760582948553 1103 4 0.3611015112898812 1103 5 0.08796700320621653 1103 6 0.26564608078331275 1103 7 0.39297486616663946 1103 8 0.27644861968276757 1103 9 0.457071864270965 1103 10-11 0.35954125065335774 1103 12-13 0.21086105550595136 1103 14-15 0.2973052385258441 1103 16-17 0.31456444122146365 1103 18-19 0.30468223082223744 1103 20-21 0.3401904104622844 1103 22-23 0.3458269400761722 1103 24-25 0.2802009484958674 1103 26-27 0.2912366085408564 1103 28-29 0.41297543542409443 1103 30-31 0.3647775602862282 1103 32-33 0.6053366528541204 1103 34-35 0.34286526421485064 1103 36-37 0.41418926869288697 1103 38-39 0.8902245774235524 1103 40-41 0.5788721450363994 1103 42-43 0.8342100474132366 1103 44-45 0.5833773530679736 1103 46-47 0.8298113196958141 1103 48-49 0.45197294949950617 1103 50-51 0.6098614024378932 1103 52-53 0.7139960744711189 1103 54-55 0.49561902771958444 1103 56-57 0.33187632203475204 1103 58-59 0.3477924833797914 1103 60-61 0.37594387984872313 1103 62-63 0.2562049039999863 1103 64-65 0.1463809355362926 1103 66-67 0.37510508809441134 1103 68-69 0.29888578291360446 1103 70-71 0.3440413994713545 1103 72-73 0.37312647434215407 1103 74-75 0.5042123282380082 1103 76-77 0.516227742609491 1103 78-79 0.29978212972946494 1103 80-81 0.42277442744435234 1103 82-83 0.485726606097753 1103 84-85 0.49011259092492665 1103 86-87 0.24998409434423507 1103 88-89 0.3224286755118513 1103 90-91 0.5750842727089811 1103 92-93 0.7392525884624952 1103 94-95 0.7240869075476404 1103 96-97 0.7590280501500288 1103 98-99 0.6193697783278012 1103 100-101 0.17122290655208872 1104 1 0.15054848032374935 1104 2 0.24902186371837232 1104 3 0.2520393547779598 1104 4 0.21817983872889357 1104 5 0.08953192498153584 1104 6 0.10163328738724431 1104 7 0.11987996040447513 1104 8 0.1732494143461878 1104 9 0.26073091255875624 1104 10-11 0.2576950283017396 1104 12-13 0.20252693721413095 1104 14-15 0.08651284434538553 1104 16-17 0.0879494248229662 1104 18-19 0.057473828626626755 1104 20-21 0.09714906741259455 1104 22-23 0.29693223297986293 1104 24-25 0.2660652734173041 1104 26-27 0.32341812234042777 1104 28-29 0.4021558531361862 1104 30-31 0.2957208377590561 1104 32-33 0.3725818339226379 1104 34-35 0.2912613799548396 1104 36-37 0.27211106970163 1104 38-39 0.6447277415198371 1104 40-41 0.6387448533144209 1104 42-43 0.27154574133080445 1104 44-45 0.22107655412295912 1104 46-47 0.4121473989566411 1104 48-49 0.26712914196561854 1104 50-51 -0.2804035424580178 1104 52-53 -0.09183267042308074 1104 54-55 -0.06149847628370253 1104 56-57 -0.15521896050748296 1104 58-59 -0.2937537278396931 1104 60-61 -0.06326608084626173 1104 62-63 -0.0034640005119541684 1104 64-65 -0.023535102449990575 1104 66-67 -0.13144788803999674 1104 68-69 -0.30126599951303135 1104 70-71 -0.1864842087287535 1104 72-73 -0.2507110735046325 1104 74-75 -0.09001801283358901 1104 76-77 -0.06153880788331989 1104 78-79 -0.10181939965509912 1104 80-81 -0.5344867239612938 1104 82-83 -0.53789279465067 1104 84-85 -0.41088095243475564 1104 86-87 -0.43236675640804023 1104 88-89 -0.3927566317779849 1104 90-91 -0.4272309980160678 1104 92-93 -0.45601368372685513 1104 94-95 -0.5214281251209059 1104 96-97 -0.6743043555051003 1104 98-99 -0.6737287239714149 1104 100-101 -1.27531499837945 1105 1 0.19648924793957434 1105 2 0.24020223101622307 1105 3 0.3947127613534427 1105 4 0.21050979531355551 1105 5 0.23658355458549352 1105 6 0.18148490068497125 1105 7 0.20864700551158677 1105 8 0.2796822224829967 1105 9 0.22685267933889008 1105 10-11 0.47627597854735626 1105 12-13 0.5166073676986329 1105 14-15 0.5445487588191966 1105 16-17 0.535964141196466 1105 18-19 0.35346962977214247 1105 20-21 0.4811750758510698 1105 22-23 0.47674409983948607 1105 24-25 0.5205668123178597 1105 26-27 0.5479100813302509 1105 28-29 0.4992951287318661 1105 30-31 0.6370856692953097 1105 32-33 0.583332735861049 1105 34-35 0.45860407578248186 1105 36-37 0.48015309901207814 1105 38-39 0.7643370034590546 1105 40-41 0.5327828875982803 1105 42-43 0.2723569763239837 1105 44-45 0.18165012579903816 1105 46-47 0.20280698600134173 1105 48-49 0.1694639904195867 1105 50-51 0.4588863211811258 1105 52-53 0.2757199681289251 1105 54-55 0.4207440381371299 1105 56-57 0.49067288841528267 1105 58-59 0.24835711376564973 1105 60-61 0.5385449299362328 1105 62-63 0.5516357732390915 1105 64-65 0.6672722598133163 1105 66-67 0.4075036213055121 1105 68-69 0.34532090250690217 1105 70-71 0.2688142972128311 1105 72-73 0.5080147983691603 1105 74-75 0.5445552734834429 1105 76-77 0.6368336264331411 1105 78-79 0.5930003145782017 1105 80-81 0.5230661184186047 1105 82-83 0.5718379487096392 1105 84-85 0.6449067404373814 1105 86-87 0.49194175748283087 1105 88-89 0.4292771628858034 1105 90-91 0.7142021158625731 1105 92-93 0.5594792740196226 1105 94-95 0.32740801764014904 1105 96-97 0.17806922024753646 1105 98-99 0.5391297583261334 1105 100-101 0.5483626681988909 1106 1 0.1269080020751261 1106 2 0.031420682665721955 1106 3 0.0367866813836919 1106 4 0.08232187585046802 1106 5 0.12068413119953902 1106 6 0.17902720146954465 1106 7 0.178398399783255 1106 8 0.22083923165210706 1106 9 0.1460794493549571 1106 10-11 0.28191923405353947 1106 12-13 0.30523578248263306 1106 14-15 0.3552586557848869 1106 16-17 0.2850094195781665 1106 18-19 0.4747807177766816 1106 20-21 0.519477372854567 1106 22-23 0.49184477098042834 1106 24-25 0.3531785903687137 1106 26-27 0.26948111829026544 1106 28-29 0.3237256042021386 1106 30-31 0.20302762978307243 1106 32-33 0.2365655555982613 1106 34-35 -0.09341407338095564 1106 36-37 -0.45817282971464124 1106 38-39 -0.026602593856381418 1106 40-41 -0.25426692807428 1106 42-43 -0.6520782254909392 1106 44-45 -0.7645546194509834 1106 46-47 -0.7971504768968529 1106 48-49 -0.5727848043625343 1106 50-51 -0.6634220255435217 1106 52-53 -0.604268688643927 1106 54-55 -0.5194866074815536 1106 56-57 -0.7685009465407617 1106 58-59 -0.727803203844708 1106 60-61 -0.5285833430574307 1106 62-63 -0.5324978877874713 1106 64-65 -0.5070353306961835 1106 66-67 -0.7198335561214648 1106 68-69 -0.7686879830210316 1106 70-71 -0.6782790008304609 1106 72-73 -0.5191285989273737 1106 74-75 -0.5764864128763243 1106 76-77 -0.4241598512112468 1106 78-79 -0.15636635523935993 1106 80-81 -0.17863536565361926 1106 82-83 -0.17034940359209116 1106 84-85 0.012531279618780644 1106 86-87 0.19666810212788377 1106 88-89 0.25318301410047184 1106 90-91 0.3402395485121623 1106 92-93 -0.33453717365974 1106 94-95 -0.11952931102986142 1106 96-97 0.08454431241125704 1106 98-99 0.3709900475784238 1106 100-101 0.26133648424956135 1107 1 0.15243976586192076 1107 2 0.20195795051842325 1107 3 0.20563011734122583 1107 4 0.10442513922889418 1107 5 0.22698894992046803 1107 6 0.2355202237343832 1107 7 0.2975880592413702 1107 8 0.21870152347413097 1107 9 0.40118757761744916 1107 10-11 0.22814273463946222 1107 12-13 0.33381431307459053 1107 14-15 0.33455360269728374 1107 16-17 0.22463691860163948 1107 18-19 0.3974352754983528 1107 20-21 0.3251705300577896 1107 22-23 0.3689398670352517 1107 24-25 0.34885878638447565 1107 26-27 0.5091814130241232 1107 28-29 0.40410360924457933 1107 30-31 0.39520475417496215 1107 32-33 0.5258228618018705 1107 34-35 0.7070484457057233 1107 36-37 0.5302313462734247 1107 38-39 0.9833846225066765 1107 40-41 0.6432251709278844 1107 42-43 0.4846436593853056 1107 44-45 0.4755300721437763 1107 46-47 0.5338711487556438 1107 48-49 0.3632899088933925 1107 50-51 0.1688255864020789 1107 52-53 0.24453284539250575 1107 54-55 0.1735329940950976 1107 56-57 0.2726499358852905 1107 58-59 0.09958485055676647 1107 60-61 0.3054388862668098 1107 62-63 0.14359493562078995 1107 64-65 0.014285822671947557 1107 66-67 -0.0036205310158230475 1107 68-69 0.0893275348938154 1107 70-71 -0.015081671190177559 1107 72-73 0.1016767702001431 1107 74-75 0.05963091250274388 1107 76-77 0.1697608192194906 1107 78-79 0.22169284010171353 1107 80-81 0.18645198919883654 1107 82-83 0.389326211620439 1107 84-85 0.4121211379334717 1107 86-87 0.5912226278904633 1107 88-89 0.4791355726033686 1107 90-91 0.5750412245890075 1107 92-93 0.44081171309854383 1107 94-95 0.4306944026166697 1107 96-97 0.6190707851927648 1107 98-99 0.5364786665905328 1107 100-101 0.10600109133027402 1108 1 0.1237841778843034 1108 2 0.23331422763741116 1108 3 0.1975314990731114 1108 4 0.22628554498760423 1108 5 0.20377785467547227 1108 6 0.2838739415796212 1108 7 0.2868922265116396 1108 8 0.21532135060243718 1108 9 0.34910877130135276 1108 10-11 0.4014628232137838 1108 12-13 0.4274140592167086 1108 14-15 0.5556419148872891 1108 16-17 0.559737944380629 1108 18-19 0.37837092804417694 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2316 54-55 -1.3440150426634716 2316 56-57 -1.3008393428671425 2316 58-59 -1.1069801731135165 2316 60-61 -0.9534862042372261 2316 62-63 -1.3471040340255485 2316 64-65 -1.3954705126107037 2316 66-67 -1.7401020124973066 2316 68-69 -1.7914396608733831 2316 70-71 -1.67848740775381 2316 72-73 -1.5430600719051206 2316 74-75 -1.5801741557233768 2316 76-77 -1.6649899465839049 2316 78-79 -1.806451130936992 2316 80-81 -1.8054068245020787 2316 82-83 -1.8795334375023707 2316 84-85 -1.9382882187916728 2316 86-87 -1.70189071101235 2316 88-89 -1.7835990417101968 2316 90-91 -1.815256742551064 2316 92-93 -2.1044347363584315 2316 94-95 -1.9554459482605218 2316 96-97 -1.8356075773803369 2316 98-99 -1.8490170170630442 2316 100-101 -1.1689390367677497 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3455.0 3 577.0 4 531.0 5 552.0 6 729.0 7 849.0 8 1152.0 9 1415.0 10 1835.0 11 3011.0 12 4992.0 13 7681.0 14 8251.0 15 10311.0 16 14277.0 17 18900.0 18 15547.0 19 8092.0 20 7147.0 21 6911.0 22 7566.0 23 8203.0 24 8941.0 25 9703.0 26 10537.0 27 12044.0 28 13858.0 29 16230.0 30 18763.0 31 22659.0 32 27211.0 33 34158.0 34 45819.0 35 74662.0 36 93386.0 37 129034.0 38 101337.0 39 12732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.83017394801801 17.54608952132092 13.44055352115268 17.183183009508387 2 13.241748359255714 24.207526089770603 40.2192735431543 22.331452007819383 3 23.48545418978938 32.94945365414331 28.88235085604906 14.682741300018249 4 11.420636223367584 16.441745182364333 38.237090049571584 33.9005285446965 5 10.931663803564955 43.05960504956581 30.7983063046025 15.210424842266738 6 24.840403340706395 35.94945908999074 19.820947373116393 19.389190196186473 7 24.017319993439397 39.80593734623585 19.74610464162703 16.43063801869772 8 25.316983934931354 29.484951957019838 21.413048898594013 23.785015209454798 9 30.23376977882883 18.889117821910816 21.294926829652397 29.58218556960796 10-11 23.25628389953109 27.738386652958685 25.08258576946318 23.922743678047045 12-13 31.630959456604884 25.890449780954665 25.315053970828068 17.16353679161238 14-15 27.427016614242767 24.964339937826175 30.553685616692718 17.054957831238347 16-17 18.205510174228873 29.648885626593568 32.235916428390105 19.909687770787457 18-19 17.82590808611489 29.284009718796604 36.8011170185892 16.088965176499304 20-21 20.07528099105751 27.139420789236198 33.87493641808187 18.910361801624415 22-23 18.49186585786878 27.502694315617582 32.216138589556955 21.78930123695669 24-25 19.451846687241947 27.777482344584087 34.08143583158207 18.689235136591893 26-27 21.604479620248345 24.748116535238744 35.60060176354526 18.04680208096765 28-29 18.633069915198615 25.54169182703668 38.12071726745255 17.704520990312165 30-31 18.166198852438683 29.969752315499953 35.805212524602084 16.05883630745928 32-33 16.694365134061893 29.726822224410093 34.521151258573724 19.057661382954286 34-35 17.74407265051407 27.299377591314556 34.82753065190158 20.129019106269787 36-37 20.38102624402692 27.365761229522985 34.943557763589126 17.30965476286097 38-39 22.15141010308966 25.119441974466834 35.65192380724214 17.077224115201364 40-41 18.074295976945308 27.608970482067658 37.11975581714453 17.19697772384251 42-43 17.254834588262707 30.16115623327666 34.65652191370641 17.927487264754223 44-45 18.022078571804727 30.632998051503503 31.870161672547265 19.474761704144505 46-47 17.53020070608201 29.683092541770158 30.569546049699593 22.217160702448247 48-49 19.193986732573663 29.20920819430802 30.95085416628262 20.6459509068357 50-51 20.97529471944131 30.349700162015868 30.418891010917143 18.256114107625674 52-53 17.895255219035217 30.65314557600807 31.74379539024999 19.707803814706722 54-55 16.186302834261806 30.19265239168199 32.43329706331324 21.18774771074297 56-57 19.035107113809904 31.92739055019043 28.20321486195887 20.834287474040792 58-59 18.06250616837188 29.437444484653092 30.19074250748429 22.309306839490738 60-61 18.61607292590807 28.163561116091014 31.226912243342408 21.99345371465851 62-63 22.075065506208276 27.731049284369362 30.21363582498321 19.980249384439148 64-65 20.27533058498697 28.68633696165965 32.21147959082907 18.82685286252431 66-67 18.54091856580588 29.1265868955773 32.27391058738016 20.058583951236663 68-69 18.31620354000365 31.40642089051221 31.09306057355671 19.184314995927434 70-71 18.110719416786612 31.115885480109405 29.034382041115176 21.73901306198881 72-73 18.30426389763064 29.627847141205564 28.459429636866872 23.60845932429692 74-75 19.00743139277877 29.965229746376643 29.863308987791235 21.16402987305335 76-77 19.919679878038746 27.905153534394746 31.674775255715126 20.500391331851382 78-79 18.37022810830307 27.90835124650677 33.25433108283323 20.46708956235693 80-81 17.444463238425193 31.542537542804755 31.295202905885816 19.71779631288424 82-83 17.23559136946744 30.65038365309361 29.463912517747698 22.65011245969125 84-85 18.272686580918656 28.44758356481786 29.423899735383564 23.855830118879922 86-87 19.75768283387069 28.206331631961223 30.3258498412945 21.71013569287359 88-89 20.95740345883361 26.061457460401684 31.489196987361325 21.491942093403384 90-91 18.997209642863258 27.347277656995452 32.42509556874431 21.230417131396983 92-93 17.99978139543459 29.66654902404914 31.138113310911002 21.195556269605273 94-95 18.14878340252241 30.128359181846015 29.807576450913064 21.91528096471851 96-97 18.746563865194876 28.351412477737135 28.398884634757387 24.503139022310595 98-99 20.15687105904754 27.584313420817512 28.61741330645294 23.641402213682007 100-101 21.723311436408842 27.357353462968213 29.78894559692763 21.130389503695312 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4725.0 1 3092.0 2 2302.5 3 2894.0 4 2089.0 5 1951.5 6 3787.5 7 7043.0 8 9764.5 9 9348.0 10 6753.0 11 5127.0 12 4726.5 13 4569.0 14 4290.5 15 3922.0 16 3575.5 17 3441.0 18 3480.5 19 3508.0 20 3627.0 21 3858.0 22 4108.0 23 4404.5 24 4836.0 25 5338.5 26 5963.5 27 6702.0 28 7401.5 29 8217.5 30 9267.5 31 10059.0 32 11051.5 33 12461.0 34 13755.5 35 14745.0 36 16125.5 37 17801.5 38 17935.0 39 16787.0 40 15709.0 41 14967.0 42 14433.5 43 14736.0 44 16423.0 45 17567.5 46 21195.5 47 31670.0 48 75208.0 49 78643.5 50 45413.5 51 33458.0 52 16448.0 53 10527.0 54 9015.0 55 8026.0 56 7594.0 57 7163.0 58 6463.5 59 6113.0 60 6012.0 61 5944.5 62 5120.5 63 3000.5 64 1454.5 65 1231.0 66 1172.0 67 941.5 68 686.0 69 550.5 70 520.5 71 456.0 72 396.0 73 519.0 74 499.5 75 314.0 76 225.0 77 183.0 78 150.5 79 109.0 80 82.0 81 65.5 82 56.5 83 40.5 84 25.5 85 22.0 86 15.5 87 7.5 88 3.5 89 2.0 90 2.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29565249299529 2 0.17836127791072237 3 0.17836127791072237 4 0.20090215946887394 5 0.21571099444603165 6 0.10798654885998181 7 0.12227117728927553 8 0.13524528934890925 9 0.14009419991665117 10-11 0.1273166653124664 12-13 0.11624280198883963 14-15 0.13118268860296334 16-17 0.10661050667183883 18-19 0.13491766025649427 20-21 0.16322481384114967 22-23 0.16630452730985062 24-25 0.1919251223367031 26-27 0.16997397314489857 28-29 0.1674839920425446 30-31 0.15483750907532587 32-33 0.16813925022737458 34-35 0.3545602038115058 36-37 0.47296535781028437 38-39 0.44288900712658796 40-41 0.4324048761693082 42-43 0.4525868282620718 44-45 0.4584186261070587 46-47 0.46058097811699766 48-49 0.4723100996254544 50-51 0.46713355996529754 52-53 0.47211352217000546 54-55 0.45573206754925577 56-57 0.4209378579347835 58-59 0.4105847786144696 60-61 0.3642580249469896 62-63 0.4702132734339985 64-65 0.4679853956055765 66-67 0.47637270037140034 68-69 0.4860049956884011 70-71 0.4821389723979042 72-73 0.4733585127211824 74-75 0.4776176909225773 76-77 0.45507680936442574 78-79 0.442299274760241 80-81 0.44013692275030203 82-83 0.45245577662510583 84-85 0.4544870769980788 86-87 0.45540443845684075 88-89 0.46189149448665767 90-91 0.4792558363846523 92-93 0.4843668502263262 94-95 0.48036977529886327 96-97 0.48036977529886327 98-99 0.4776176909225773 100-101 0.46058097811699766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 763058.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.848853476227756 #Duplication Level Percentage of deduplicated Percentage of total 1 75.02107691481426 23.89335286288732 2 11.669774041872392 7.433378471205598 3 4.7386981074853916 4.527663050801401 4 2.458301066592105 3.1317628188138547 5 1.493001672506001 2.3775195753703295 6 0.929302725700088 1.7758335807528705 7 0.6651591003114662 1.4829188306939558 8 0.44781687991482383 1.1409963354070962 9 0.35394514972309465 1.0145472490937102 >10 1.9034598407847627 11.360980991557414 >50 0.1745527682331522 3.810353260948223 >100 0.10456698847691055 6.395299218919975 >500 0.018113966507811278 3.9245550315875164 >1k 0.01687892333682415 11.18666012094131 >5k 0.003293448455965687 6.965652499268956 >10k+ 0.0020584052849785543 9.578526101750448 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19330 2.5332281425527285 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16244 2.128802790875661 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16079 2.107179270776271 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11353 1.4878292344749677 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10048 1.3168068482343414 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8509 1.1151183789436714 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7382 0.9674231840829923 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7257 0.9510417294622427 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6704 0.8785701742200462 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6621 0.8676928883518684 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6168 0.8083264968062717 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5300 0.6945736759197859 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5185 0.6795027376686963 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4700 0.6159426937401875 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3457 0.4530455089914528 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3381 0.44308558458203706 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3379 0.442823481308105 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3252 0.42617992341342337 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3183 0.4171373604627695 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3138 0.41124003679929966 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3107 0.4071774360533537 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3079 0.4035079902183058 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3075 0.4029837836704418 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2784 0.36484775731333663 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2646 0.34676263141202895 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2626 0.34414159867270905 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2564 0.3360163971808172 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2525 0.33090538333914327 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2179 0.28556151694890824 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2111 0.2766500056352204 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 0.2661658746779406 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1892 0.24794969713966697 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1844 0.24165921856529912 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1828 0.23956239237384316 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1822 0.23877608255204716 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1724 0.2259330221293794 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1700 0.22278778284219547 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1606 0.21046892896739175 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1546 0.2026058307494319 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1454 0.19054908014856015 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1446 0.18950066705283214 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1388 0.1818996721088043 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1373 0.17993389755431435 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1307 0.17128448951455852 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1254 0.16433875275536067 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1253 0.16420770111839467 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1159 0.15188884724359092 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1149 0.15057833087393094 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1102 0.14441890393652906 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1086 0.1423220777450731 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1073 0.14061840646451515 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1057 0.1385215802730592 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1029 0.13485213443801125 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1010 0.13236215333565732 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 997 0.13065848205509933 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 893 0.11702911181063562 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 876 0.11480123398221367 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 847 0.11100073651019976 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 830 0.1087728586817778 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 821 0.10759339394908382 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 819 0.10733129067515182 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 813 0.10654498085335584 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 809 0.10602077430549184 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 806 0.10562761939459386 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 778 0.10195817355954594 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 774 0.10143396701168195 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3105163696599734E-4 0.0 16-17 0.0 0.0 0.0 1.3105163696599734E-4 0.0 18-19 0.0 0.0 0.0 5.89732366346988E-4 0.0 20-21 0.0 0.0 0.0 0.0019002487360069613 0.0 22-23 0.0 0.0 0.0 0.003734971653530924 0.0 24-25 0.0 0.0 0.0 0.006618107666782866 0.0 26-27 0.0 0.0 0.0 0.01330174115204873 0.0 28-29 0.0 0.0 0.0 0.03151791869032236 0.0 30-31 0.0 0.0 0.0 0.07542021707393147 0.0 32-33 0.0 0.0 0.0 0.15509961234925784 0.0 34-35 0.0 0.0 0.0 0.296111173724671 0.0 36-37 0.0 0.0 0.0 0.493081784084565 0.0 38-39 0.0 0.0 0.0 0.7511879830890967 0.0 40-41 0.0 0.0 0.0 1.0556209357611086 0.0 42-43 0.0 0.0 0.0 1.3110405762078374 0.0 44-45 0.0 0.0 0.0 1.5692778268493353 0.0 46-47 0.0 0.0 0.0 1.9012971491026893 0.0 48-49 0.0 0.0 0.0 2.2907826141656336 0.0 50-51 0.0 0.0 0.0 2.6977634727635382 0.0 52-53 0.0 0.0 0.0 3.073750619218985 0.0 54-55 0.0 0.0 0.0 3.4456751649284847 0.0 56-57 0.0 0.0 0.0 3.829132254690993 0.0 58-59 0.0 0.0 0.0 4.3549769480170575 0.0 60-61 0.0 0.0 0.0 5.083755101184969 0.0 62-63 0.0 0.0 0.0 5.692227851618094 6.552581848299867E-5 64-65 0.0 0.0 0.0 6.2030016066930695 1.3105163696599734E-4 66-67 0.0 0.0 0.0 6.762526570719395 1.3105163696599734E-4 68-69 0.0 0.0 0.0 7.393802305984604 1.3105163696599734E-4 70-71 0.0 0.0 0.0 8.16202700187928 1.3105163696599734E-4 72-73 0.0 0.0 0.0 8.975464512527225 1.3105163696599734E-4 74-75 0.0 0.0 0.0 9.744541044062181 1.3105163696599734E-4 76-77 0.0 0.0 0.0 10.442325485087634 1.3105163696599734E-4 78-79 0.0 0.0 0.0 11.194692932909426 1.3105163696599734E-4 80-81 0.0 0.0 0.0 11.99083162747786 1.3105163696599734E-4 82-83 0.0 0.0 0.0 12.877002796641932 1.3105163696599734E-4 84-85 0.0 0.0 0.0 14.023770145912893 1.3105163696599734E-4 86-87 0.0 0.0 0.0 15.252837923198499 1.3105163696599734E-4 88-89 0.0 0.0 0.0 16.20892514068393 1.3105163696599734E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGCTA 45 9.729091E-4 42.12828 9 ATACGCT 55 0.0026074061 34.468594 8 ACGTTTA 30 0.003997879 31.591026 12-13 TCCCGCT 170 1.10958354E-10 30.66691 8 CTATTAC 110 9.531518E-5 25.88543 1 ATGGGAG 2280 0.0 25.162157 5 CGCTGTA 105 1.3005774E-9 24.822334 10-11 GTGGTAC 250 1.4551915E-11 24.677443 1 CCCGCTG 215 1.7025741E-9 24.248255 9 TCGCCGG 50 0.0016443459 23.773626 94-95 CATATAG 100 0.0016619478 23.728312 1 GTCACTA 200 2.071647E-8 23.728312 1 ATGGGGG 910 0.0 23.445984 5 TACCTCC 365 0.0 23.381748 2 TACTCCC 245 3.0377123E-10 23.222689 5 AGTACTT 9270 0.0 23.10541 12-13 GGGAACG 1090 0.0 23.044943 7 GTGTAGC 125 2.268922E-4 22.779179 1 GTCGGGA 550 0.0 22.413414 2 GAGTACT 9210 0.0 22.40699 12-13 >>END_MODULE