##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939263_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 763058 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.111333607668094 33.0 31.0 34.0 30.0 34.0 2 32.249886640334026 33.0 31.0 34.0 30.0 34.0 3 32.3173009129057 34.0 31.0 34.0 30.0 34.0 4 35.87230852700581 37.0 35.0 37.0 35.0 37.0 5 35.846205661954926 37.0 35.0 37.0 35.0 37.0 6 35.96231610179043 37.0 35.0 37.0 35.0 37.0 7 35.896000041936524 37.0 35.0 37.0 35.0 37.0 8 35.92750747649589 37.0 35.0 37.0 35.0 37.0 9 37.51021023303602 39.0 37.0 39.0 35.0 39.0 10-11 37.443504294562146 39.0 37.0 39.0 34.0 39.0 12-13 37.356538428271506 39.0 37.0 39.0 34.0 39.0 14-15 38.30756377627913 40.0 38.0 41.0 33.5 41.0 16-17 38.350399314337835 40.0 37.5 41.0 33.5 41.0 18-19 38.6882491239198 40.0 38.0 41.0 34.0 41.0 20-21 38.719311638171675 40.0 38.0 41.0 34.0 41.0 22-23 38.64689577987519 40.0 38.0 41.0 34.0 41.0 24-25 38.530730298352154 40.0 38.0 41.0 34.0 41.0 26-27 38.13492093654742 40.0 37.0 41.0 33.5 41.0 28-29 37.836366960309704 40.0 36.5 41.0 33.0 41.0 30-31 37.48765690157236 39.0 36.0 41.0 33.0 41.0 32-33 37.11287346440244 39.0 35.0 41.0 33.0 41.0 34-35 36.91432040552618 39.0 35.0 41.0 32.5 41.0 36-37 36.55811091686346 39.0 35.0 41.0 31.0 41.0 38-39 36.18549310799442 39.0 35.0 41.0 30.5 41.0 40-41 35.77106065331862 38.0 35.0 41.0 30.0 41.0 42-43 35.447528890333366 38.0 35.0 41.0 29.0 41.0 44-45 34.90571293401026 38.0 35.0 41.0 25.0 41.0 46-47 34.09022970730928 38.0 34.0 40.0 20.0 41.0 48-49 33.4193259228001 38.0 33.5 40.0 5.5 41.0 50-51 33.05075433322238 38.0 33.0 40.0 2.0 41.0 52-53 32.58590696906395 37.5 33.0 40.0 2.0 41.0 54-55 32.332691617150985 37.0 33.0 40.0 2.0 41.0 56-57 32.10585302821018 37.0 32.5 40.0 2.0 41.0 58-59 31.847911299010036 37.0 32.0 40.0 2.0 41.0 60-61 31.417282172521617 36.0 31.0 39.5 2.0 41.0 62-63 30.90421435854155 35.5 31.0 39.0 2.0 40.5 64-65 30.47787717316377 35.0 30.0 39.0 2.0 40.0 66-67 30.018504491139602 35.0 29.5 38.0 2.0 40.0 68-69 29.594414841335784 35.0 29.0 37.5 2.0 40.0 70-71 29.051803401576286 35.0 29.0 37.0 2.0 39.0 72-73 28.48807757732702 34.5 27.5 36.5 2.0 39.0 74-75 27.958942832654923 34.0 26.0 36.0 2.0 38.0 76-77 26.028142683780263 31.5 24.0 34.5 2.0 36.0 78-79 26.801041336307332 33.0 25.5 35.0 2.0 36.0 80-81 26.846875991078004 34.0 25.0 35.0 2.0 36.0 82-83 26.528067984347192 33.5 25.0 35.0 2.0 36.0 84-85 26.137828972371693 33.0 23.5 35.0 2.0 35.5 86-87 25.517914103515068 33.0 20.0 35.0 2.0 35.0 88-89 24.854923872104088 32.5 14.5 35.0 2.0 35.0 90-91 24.271355519501796 32.0 6.5 34.5 2.0 35.0 92-93 23.9306946523069 31.5 2.0 34.0 2.0 35.0 94-95 23.503170794356393 31.0 2.0 34.0 2.0 35.0 96-97 22.76119770712056 31.0 2.0 34.0 2.0 35.0 98-99 21.880730560455433 31.0 2.0 34.0 2.0 35.0 100-101 18.251558203963526 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1575425396061334 1101 2 0.3309240919244161 1101 3 0.34318502161173114 1101 4 0.21680802269767696 1101 5 0.291987685169282 1101 6 0.34143469300081364 1101 7 0.31437132505099896 1101 8 0.1649404752215986 1101 9 0.37183369245279607 1101 10-11 0.3448184831631309 1101 12-13 0.47182411284697423 1101 14-15 0.6225479717420725 1101 16-17 0.4372399171623371 1101 18-19 0.46995995071615226 1101 20-21 0.4659605828402036 1101 22-23 0.39822795816942147 1101 24-25 0.6224368648367289 1101 26-27 0.5770296437814011 1101 28-29 0.47934216376250305 1101 30-31 0.6232210329694965 1101 32-33 0.5576678416637719 1101 34-35 0.5917654978278719 1101 36-37 0.42935261758466226 1101 38-39 0.6157640602118661 1101 40-41 0.8294945439369101 1101 42-43 1.0094395185198977 1101 44-45 1.1325262610380662 1101 46-47 0.9810550621216763 1101 48-49 1.1071832266585417 1101 50-51 1.0613734869265912 1101 52-53 1.0305130353075427 1101 54-55 1.0466004830669107 1101 56-57 0.9176037933327237 1101 58-59 1.1057402426345853 1101 60-61 1.0433239367241107 1101 62-63 1.2481641005596096 1101 64-65 1.2311099204560172 1101 66-67 1.053529167990316 1101 68-69 1.1770946314466286 1101 70-71 0.8210704304085503 1101 72-73 0.8948343241486327 1101 74-75 0.9215037349665813 1101 76-77 1.0607159681398883 1101 78-79 1.1625575576193405 1101 80-81 1.2042050417127577 1101 82-83 1.276920576026619 1101 84-85 1.096790801442765 1101 86-87 1.0929782461421098 1101 88-89 1.3016709169540768 1101 90-91 1.3571736271982537 1101 92-93 1.2076591388174727 1101 94-95 1.0652814880540404 1101 96-97 0.9521515619707408 1101 98-99 1.670213313897733 1101 100-101 1.2378292370988504 1102 1 0.12086162792522259 1102 2 0.20123161664371025 1102 3 0.24932853314739845 1102 4 0.1171557686336584 1102 5 0.20758597576757154 1102 6 0.2455461951515261 1102 7 0.18071957375219228 1102 8 0.2969093596414609 1102 9 0.4265582888440562 1102 10-11 0.2740088915202037 1102 12-13 0.37354748025661877 1102 14-15 0.4479767187394472 1102 16-17 0.3424931153076969 1102 18-19 0.43016817930674023 1102 20-21 0.438545913268662 1102 22-23 0.4656473760790405 1102 24-25 0.34725321587856683 1102 26-27 0.5945950032585259 1102 28-29 0.3406802175025163 1102 30-31 0.6394805994741546 1102 32-33 0.6771758300443125 1102 34-35 0.6243788993138182 1102 36-37 0.8231853643585865 1102 38-39 0.8564908352523659 1102 40-41 0.964249585554704 1102 42-43 1.1953998000684152 1102 44-45 1.3522172000917507 1102 46-47 1.2848474480219068 1102 48-49 1.257223727189242 1102 50-51 1.1791916255780635 1102 52-53 1.2045710488263452 1102 54-55 1.1394374754137075 1102 56-57 1.1801065864727747 1102 58-59 0.9879535268086563 1102 60-61 1.2209920837550037 1102 62-63 1.1438088969396922 1102 64-65 0.9111557279528064 1102 66-67 0.9805199785595597 1102 68-69 0.9396951844883574 1102 70-71 0.8208050821039841 1102 72-73 0.8200375250871268 1102 74-75 0.8958417607751947 1102 76-77 0.8180339950657576 1102 78-79 0.9798477928017313 1102 80-81 1.0551735630244181 1102 82-83 1.2204886727809914 1102 84-85 1.0813691769525136 1102 86-87 0.885088323791404 1102 88-89 0.8032141268306212 1102 90-91 1.1489485280417817 1102 92-93 1.2302661160813138 1102 94-95 1.4552457358614213 1102 96-97 0.5988856829695663 1102 98-99 0.2515041635709352 1102 100-101 -0.1133382954412312 1103 1 0.24679303270277586 1103 2 0.17002955659549457 1103 3 0.19699636863575165 1103 4 0.22385640418044517 1103 5 0.24021253724028924 1103 6 0.25975468371899524 1103 7 0.21386662613001306 1103 8 0.03429886946686622 1103 9 0.2081256056677887 1103 10-11 0.19688948908029147 1103 12-13 0.3481438715196674 1103 14-15 0.3156533203904104 1103 16-17 0.2464260176380364 1103 18-19 0.34961536317700137 1103 20-21 0.4470198704605721 1103 22-23 0.42998918336956393 1103 24-25 0.3142659633400342 1103 26-27 0.20139151870888128 1103 28-29 0.27599780826881926 1103 30-31 0.5274152550370133 1103 32-33 0.40374917360482954 1103 34-35 0.28474032089319223 1103 36-37 0.38323357825296256 1103 38-39 0.3289413429592827 1103 40-41 0.5009761543325553 1103 42-43 0.4634800104563155 1103 44-45 0.38956013967827374 1103 46-47 0.4393738926124513 1103 48-49 0.12760322897899812 1103 50-51 0.14026895043743792 1103 52-53 0.004451427232361027 1103 54-55 0.09620020461233736 1103 56-57 -0.03888767899584877 1103 58-59 0.27321906848445465 1103 60-61 0.23748799287911382 1103 62-63 0.44694006064778335 1103 64-65 0.5834564803817663 1103 66-67 0.5030882815765807 1103 68-69 0.46233653886048387 1103 70-71 0.4629082306173906 1103 72-73 0.4646655110612663 1103 74-75 0.5079116709220308 1103 76-77 0.5215002818653787 1103 78-79 0.5839231452745217 1103 80-81 0.8006489447177465 1103 82-83 0.8519582834172681 1103 84-85 1.222472435042949 1103 86-87 1.4457163828169008 1103 88-89 1.4752409366779453 1103 90-91 1.4957917963594092 1103 92-93 0.9563828886724437 1103 94-95 1.0971297303095184 1103 96-97 0.9668864500087935 1103 98-99 0.46234407152622836 1103 100-101 0.6047656566370776 1104 1 0.05783568591953525 1104 2 0.14897911088179683 1104 3 0.039271092687165776 1104 4 0.10876079623723456 1104 5 0.1815568714099598 1104 6 0.1401612636691567 1104 7 0.11996489722326942 1104 8 0.08125080431816656 1104 9 0.07497666621805621 1104 10-11 -0.09818496779980279 1104 12-13 -0.10187282668805153 1104 14-15 0.1329117743932713 1104 16-17 -0.0694088071164245 1104 18-19 0.010382710045867327 1104 20-21 0.07299500297583705 1104 22-23 -0.0637361439581241 1104 24-25 0.010274021295657576 1104 26-27 0.1771544983541986 1104 28-29 0.27513399713840414 1104 30-31 0.038125656735957136 1104 32-33 0.15030142186864737 1104 34-35 0.42579950105400854 1104 36-37 0.2652714446501676 1104 38-39 0.21651209626252665 1104 40-41 0.13427066790091402 1104 42-43 0.3595540049244974 1104 44-45 0.18302578846787299 1104 46-47 0.07290175292980194 1104 48-49 -0.4223715096298264 1104 50-51 -0.3633918104870446 1104 52-53 -0.126371537513009 1104 54-55 -0.18079309041502256 1104 56-57 -0.07977687996520189 1104 58-59 -0.26974242287958106 1104 60-61 -0.27921449643831053 1104 62-63 -0.2446076491288025 1104 64-65 -0.37523365226194727 1104 66-67 -0.3123001872405844 1104 68-69 -0.31326952044812373 1104 70-71 -0.16148485375565613 1104 72-73 -0.0636689925738061 1104 74-75 -0.0306804560871079 1104 76-77 0.11027908627370309 1104 78-79 0.15422045900387715 1104 80-81 0.02948503682334902 1104 82-83 0.10730170988949084 1104 84-85 0.13362726596289676 1104 86-87 -0.15063441655627585 1104 88-89 -0.04878735608017237 1104 90-91 -0.1563781143265892 1104 92-93 0.04772850731963274 1104 94-95 0.35013326690428315 1104 96-97 0.1893108585069001 1104 98-99 0.29004532774523994 1104 100-101 0.25390098918830617 1105 1 0.07971660966752836 1105 2 0.06039958743139806 1105 3 0.09825480683893062 1105 4 0.18939103206610497 1105 5 0.2930711087879203 1105 6 0.18616521793017426 1105 7 0.19094388256804962 1105 8 0.14839171053695566 1105 9 0.1194682175521109 1105 10-11 0.15895716138631855 1105 12-13 0.31314928863776714 1105 14-15 0.2893782141315526 1105 16-17 0.10917499671962361 1105 18-19 0.2917813770114819 1105 20-21 0.3192883928374428 1105 22-23 0.3570706068215159 1105 24-25 0.24511228386674588 1105 26-27 0.17970846178747735 1105 28-29 0.5052189933675848 1105 30-31 0.486770201282539 1105 32-33 0.38623729761516756 1105 34-35 0.44742054424514066 1105 36-37 0.28056668934554807 1105 38-39 0.4205927305749171 1105 40-41 0.5080767460485234 1105 42-43 0.22572061383993258 1105 44-45 0.23929553429449868 1105 46-47 0.4356277471003267 1105 48-49 0.5181566084272831 1105 50-51 0.6843059535210259 1105 52-53 0.826934550825996 1105 54-55 0.79989217894353 1105 56-57 0.7051826601990783 1105 58-59 0.6762859907279655 1105 60-61 0.5535059943792398 1105 62-63 0.50889105656411 1105 64-65 0.7876380788482997 1105 66-67 0.8878078415399138 1105 68-69 0.7982436977903937 1105 70-71 0.9658668104990475 1105 72-73 0.6084504098028312 1105 74-75 0.7427770763724801 1105 76-77 0.6819011486042754 1105 78-79 0.7868400550170023 1105 80-81 0.9494071745702328 1105 82-83 0.813605087317594 1105 84-85 0.7453993456188321 1105 86-87 0.6808651452137653 1105 88-89 0.7871169263437494 1105 90-91 1.0184603520723847 1105 92-93 1.0904273923810877 1105 94-95 1.1331452316012935 1105 96-97 0.8383549057134374 1105 98-99 0.9622044765599824 1105 100-101 1.1035718071496206 1106 1 -0.013099346471996398 1106 2 0.03699472874910015 1106 3 0.07384796309188602 1106 4 0.02782566671965725 1106 5 -0.04843564988492233 1106 6 -0.0016408128855900372 1106 7 -0.013886441659195725 1106 8 -0.012625398830657275 1106 9 0.24352531179542325 1106 10-11 0.04719328956478108 1106 12-13 0.08717329846856359 1106 14-15 0.14389187326758446 1106 16-17 0.14036887137695686 1106 18-19 0.1486960192271951 1106 20-21 0.09524075128341991 1106 22-23 0.1559551894852902 1106 24-25 -0.0193834151596306 1106 26-27 0.13878776985061592 1106 28-29 0.08184249275316091 1106 30-31 0.25056167495679915 1106 32-33 0.19111961163350344 1106 34-35 0.3428218109054981 1106 36-37 0.27282493681695286 1106 38-39 0.34723986168371823 1106 40-41 0.3953250681961791 1106 42-43 0.4983267202772126 1106 44-45 0.8242043158428487 1106 46-47 0.8523022910080655 1106 48-49 0.8122344985870313 1106 50-51 0.7555225146326663 1106 52-53 0.8740703859710948 1106 54-55 0.6819037112244644 1106 56-57 0.798064785163632 1106 58-59 0.8326051135184009 1106 60-61 0.9678362713430353 1106 62-63 1.0457943555526725 1106 64-65 1.1806478151182702 1106 66-67 1.0820227956943675 1106 68-69 1.1307373172251225 1106 70-71 0.9202292266064269 1106 72-73 0.9609553724647064 1106 74-75 1.0603023623163317 1106 76-77 0.9459194868214986 1106 78-79 1.0936507176505224 1106 80-81 1.261828008297428 1106 82-83 1.232004747020781 1106 84-85 1.2395150465357467 1106 86-87 1.3026819520953232 1106 88-89 1.440373377804189 1106 90-91 1.4439967921895978 1106 92-93 1.5247737389166787 1106 94-95 1.4548438798667256 1106 96-97 1.5215149397431205 1106 98-99 1.2642651628252253 1106 100-101 1.4194523251569926 1107 1 0.15856004062363382 1107 2 0.0709406113527038 1107 3 0.13799696348250023 1107 4 0.10062137709926589 1107 5 0.18046957365117322 1107 6 0.12164848792048844 1107 7 0.14368253671515419 1107 8 0.1630910174897835 1107 9 0.1490009696200758 1107 10-11 0.1515441648888114 1107 12-13 0.17557569895149783 1107 14-15 0.31349700092639665 1107 16-17 0.28307953506077865 1107 18-19 0.25060468724324636 1107 20-21 0.2787134617695486 1107 22-23 0.14576201452700843 1107 24-25 0.1037567432154276 1107 26-27 0.23579870696222827 1107 28-29 0.23937951203514984 1107 30-31 0.4259664901267115 1107 32-33 0.29214496584678074 1107 34-35 0.3636866594549204 1107 36-37 0.1895874807607072 1107 38-39 0.22708166417652365 1107 40-41 0.1346640476358303 1107 42-43 0.21788657255518729 1107 44-45 0.11555494259616239 1107 46-47 -0.18153262252483415 1107 48-49 -0.04480449150564425 1107 50-51 -0.008528832975734701 1107 52-53 0.05697404706267406 1107 54-55 0.12532812797103077 1107 56-57 0.10063127783080006 1107 58-59 0.09267133985979825 1107 60-61 0.17612876805835143 1107 62-63 0.07866339429419256 1107 64-65 0.13580299426674003 1107 66-67 0.09019810907102155 1107 68-69 0.1781211104142848 1107 70-71 0.34748056711280384 1107 72-73 0.20065921820882338 1107 74-75 0.17163541156884676 1107 76-77 0.08148196684706122 1107 78-79 0.06262997975058582 1107 80-81 -0.0057832270990409995 1107 82-83 0.29136609782508316 1107 84-85 0.32464519106186174 1107 86-87 0.3838758017455497 1107 88-89 0.6727026629858237 1107 90-91 0.8487942070541266 1107 92-93 0.5742916892735508 1107 94-95 0.5798048187538392 1107 96-97 0.5416766882605728 1107 98-99 1.0600359096026821 1107 100-101 1.1199068544705852 1108 1 -0.02020626526131508 1108 2 0.02307839823625102 1108 3 -0.08522206482127359 1108 4 0.08136818835455273 1108 5 -0.06785586111494268 1108 6 -0.13408745849342552 1108 7 0.1311517529979298 1108 8 0.08996028757939456 1108 9 0.002429085760056182 1108 10-11 -0.021543967945085285 1108 12-13 0.07369797842971337 1108 14-15 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sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 17.0 5 56.0 6 162.0 7 314.0 8 627.0 9 1098.0 10 1926.0 11 3017.0 12 4723.0 13 6692.0 14 7882.0 15 10517.0 16 14066.0 17 19764.0 18 17458.0 19 9061.0 20 7562.0 21 7471.0 22 8058.0 23 8731.0 24 9246.0 25 10066.0 26 11231.0 27 12587.0 28 14917.0 29 17489.0 30 20341.0 31 24226.0 32 29024.0 33 36256.0 34 50079.0 35 77712.0 36 100507.0 37 134800.0 38 81154.0 39 4216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.54960509661299 18.203015434682886 14.029355208006109 17.218024260698012 2 13.541041441148641 24.787892925570535 39.45126058569598 22.21980504758485 3 23.517216253548224 32.56174497875653 29.369589205538766 14.551449562156481 4 11.712346898925114 17.143126734796045 37.40253034500654 33.7419960212723 5 10.703642449197833 43.40194323367293 30.91717798647023 14.977236330659006 6 25.109755745959024 36.31126336399068 18.973918103211027 19.60506278683927 7 23.656655195280045 39.78662172469196 19.56902358667362 16.987699493354373 8 25.522306299127983 30.26991395149517 20.936416366776836 23.271363382600015 9 29.60784108154295 18.881788802423934 21.047548154924005 30.462821961109114 10-11 23.219204831087545 27.980441853699194 24.844900387650743 23.95545292756252 12-13 31.624267093720267 25.983804638703745 25.375921620636966 17.016006646939026 14-15 27.32393332538277 25.25099960815098 30.344381553478218 17.080685512988026 16-17 17.980854478176738 29.87181058134422 32.29438942769843 19.85294551278061 18-19 17.77087572037494 29.175520848690955 36.71269858431376 16.34090484662034 20-21 19.836434451902736 27.131948030162846 34.087054981403774 18.944562536530643 22-23 18.411247899897518 27.327346020879144 32.43632856218007 21.825077517043265 24-25 19.48724736520684 27.803587669613584 34.261026029476135 18.448138935703444 26-27 21.483126424369345 24.86432861503138 36.009171005573634 17.64337395502564 28-29 18.434312988003533 25.468444076334958 38.38476236406669 17.712480571594817 30-31 17.97976038518697 29.94739587292185 36.0024401814803 16.070403560410874 32-33 16.57639393073659 29.71124082310912 34.858687019859566 18.853678226294722 34-35 17.608598216087714 27.213180925820595 35.22800841893369 19.950212439158 36-37 20.425738824093745 27.272828547200994 35.13085793895961 17.17057468974565 38-39 22.042552410733183 24.92079333137187 36.03305481855038 17.003599439344566 40-41 18.000281770435727 27.467113130829944 37.345853551454226 17.186751547280096 42-43 17.21545811543197 29.91748306643488 35.00479322932605 17.862265588807105 44-45 18.056042456128164 30.41937811129466 32.019870348661584 19.504709083915593 46-47 17.571973747667275 29.589702459583567 30.585566459080333 22.252757333668825 48-49 19.232084796206816 28.965484704207967 31.024122832883165 20.77830766670205 50-51 20.83396128991674 30.316542538761848 30.34878128370489 18.50071488761652 52-53 17.846766362145946 30.59188238886479 31.615462540806256 19.945888708183006 54-55 16.077021668077656 30.104264682370147 32.26150567846743 21.557207971084765 56-57 19.072434822551294 31.87327460859457 27.91502277369221 21.139267795161924 58-59 18.04730276970337 29.502111286141524 29.948941211623996 22.501644732531105 60-61 18.620540526372693 28.453612300595303 30.700443285357164 22.225403887674833 62-63 21.955878296071614 27.980003892282074 29.826347866485552 20.23776994516076 64-65 20.12968886990094 28.814230129597135 31.743827787439212 19.312253213062718 66-67 18.263017116766463 29.29540936212819 31.95813396972425 20.483439551381096 68-69 18.112276324101035 31.23966313957481 30.697887152281776 19.950173384042376 70-71 17.804752590728746 31.082913148746982 28.631525147187464 22.480809113336804 72-73 17.975501206985577 29.382104636869016 28.03482828304008 24.607565873105322 74-75 18.542299536863517 29.8654885998181 29.185854810512442 22.406357052805948 76-77 19.09584248353948 27.767905883218518 31.641505926311797 21.494745706930203 78-79 17.546276062126463 27.57194257568083 33.02573269476113 21.856048667431573 80-81 16.514570022010165 31.20280084109106 30.969135313046937 21.313493823851836 82-83 16.173148043792214 30.45502438870964 29.246728295883145 24.125099271615003 84-85 17.078190648679392 28.045377939815847 29.040256441843216 25.836174969661545 86-87 18.305391370055933 27.82046127033333 30.09959141479106 23.774555944819678 88-89 19.273684785089113 25.77181349290119 31.438497915635004 23.51600380637469 90-91 17.284084451058266 26.907063758775084 32.22194847132021 23.586903318846435 92-93 15.772457663768677 29.09700180064949 31.1219461692296 24.008594366352227 94-95 15.591540878937119 29.453069098286104 29.949034018383923 25.00635600439285 96-97 15.954684964969898 28.014973960039736 28.67167371287635 27.35866736211402 98-99 17.267768156397125 27.055235024883444 28.96976964383418 26.707227174885244 100-101 18.249196814648474 27.12024223663941 30.12375901883939 24.50680192987273 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3434.0 1 2343.5 2 2039.0 3 2712.5 4 2072.0 5 1899.0 6 3561.5 7 6817.0 8 9332.5 9 8846.0 10 6572.0 11 5015.5 12 4569.5 13 4360.5 14 4134.5 15 3805.5 16 3484.0 17 3315.0 18 3334.5 19 3383.0 20 3468.0 21 3678.5 22 3875.5 23 4108.0 24 4459.5 25 4979.5 26 5688.0 27 6438.5 28 6975.5 29 7806.0 30 8956.0 31 9778.5 32 10848.0 33 12165.0 34 13626.5 35 14771.5 36 16245.0 37 18065.0 38 18338.5 39 17478.5 40 16586.0 41 15806.0 42 15301.5 43 15648.0 44 17369.0 45 18477.5 46 21461.0 47 31495.0 48 73306.0 49 77662.5 50 48788.0 51 37555.5 52 17976.0 53 10037.0 54 8182.0 55 7501.0 56 7350.0 57 6970.0 58 6345.5 59 6132.5 60 5996.0 61 5699.0 62 4756.5 63 2758.0 64 1336.0 65 1145.5 66 1093.0 67 838.5 68 632.5 69 527.5 70 488.0 71 418.0 72 367.5 73 504.0 74 493.0 75 282.5 76 173.5 77 155.0 78 136.5 79 89.0 80 59.5 81 41.0 82 24.5 83 15.0 84 8.0 85 2.0 86 2.0 87 2.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.293424615166868 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001179464732693976 16-17 9.8288727724498E-4 18-19 6.552581848299868E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 1.3105163696599737E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.002096826191455958 36-37 0.007207840033129855 38-39 0.00294866183173494 40-41 0.003341816742632932 42-43 3.2762909241499337E-4 44-45 0.0015070938251089694 46-47 2.6210327393199473E-4 48-49 6.552581848299867E-4 50-51 1.3105163696599737E-4 52-53 1.3105163696599737E-4 54-55 0.0 56-57 8.518356402789827E-4 58-59 0.002096826191455958 60-61 7.207840033129854E-4 62-63 0.0012449905511769748 64-65 1.3105163696599737E-4 66-67 6.552581848299867E-4 68-69 0.001572619643591968 70-71 6.552581848299868E-5 72-73 0.0 74-75 0.0 76-77 0.2514225655192659 78-79 1.96577455448996E-4 80-81 1.96577455448996E-4 82-83 0.0 84-85 0.0 86-87 0.1039894739325189 88-89 0.3609817340228397 90-91 0.2654450906746276 92-93 0.0 94-95 0.0 96-97 0.0 98-99 6.552581848299868E-5 100-101 3.2762909241499337E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 763058.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.67437805055374 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90006086121466 22.668384048158284 2 12.260355418067082 7.521575542558891 3 4.927716216632711 4.534638904645086 4 2.494552391433454 3.060753724869708 5 1.5071885038406179 2.3116034980127798 6 0.9666842632248568 1.7791463127408158 7 0.6861283521681418 1.4732592325926341 8 0.5054173945379259 1.2402691386705773 9 0.37686422763946803 1.0404068213108688 >10 2.0292034974634183 11.571254544118743 >50 0.18979977746301238 4.009699011534531 >100 0.11199896776560687 6.481684548764348 >500 0.020946371834025715 4.408321758510016 >1k 0.01752655602438886 11.33124497342702 >5k 0.003419815809636851 7.0730623747222925 >10k+ 0.002137384881023032 9.494695565363388 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19301 2.5294276450807147 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17171 2.25028765834314 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15097 1.978486563275662 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10466 1.3715864324861282 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 10374 1.3595296818852565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8896 1.1658353624495124 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7819 1.0246927494371332 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7001 0.9174925103989474 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6688 0.8764733480285901 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6429 0.8425309740543969 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6260 0.8203832474071434 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5757 0.7544642740132468 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5091 0.6671838837938925 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4629 0.6066380275156017 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3711 0.4863326247808161 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3446 0.45160394098482687 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3411 0.4470171336910169 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3326 0.43587774454890715 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3221 0.4221173226674774 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3203 0.41975839320208946 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.4142542244495176 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3147 0.4124195015319936 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2984 0.3910580847065361 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2853 0.3738903202639904 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2788 0.3653719638612006 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2682 0.3514804903428049 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2666 0.3493836641513489 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2439 0.3196349425600675 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2231 0.29237620207114007 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2159 0.2829404842095883 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2061 0.27009742378692053 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1987 0.2603996026514367 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1869 0.244935509489449 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1850 0.2424455283870951 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1818 0.23825187600418318 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1788 0.23432032689520324 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1761 0.23078193269712133 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1658 0.2172836140896236 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1633 0.2140073231654737 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1470 0.1926459063400161 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1427 0.1870106859504782 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1420 0.18609332449171623 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1404 0.18399649830026027 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1308 0.17141554115152452 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1254 0.16433875275536067 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1179 0.15450987998291088 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1146 0.15018517596303296 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1094 0.14337049084080108 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1084 0.14205997447114113 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1081 0.14166681956024313 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1019 0.1335416180683513 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1018 0.1334105664313853 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1015 0.1330174115204873 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1014 0.1328863598835213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 976 0.1279063976788134 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 948 0.12423695184376549 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 932 0.12214012565230953 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 906 0.1187327830911936 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 887 0.11624280198883963 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 877 0.11493228561917966 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 844 0.11060758159930174 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 820 0.10746234231211783 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 813 0.10654498085335584 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 789 0.1033997415661719 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 786 0.10300658665527392 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 784 0.10274448338134191 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 771 0.10104081210078396 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.3105163696599734E-4 0.0 16-17 0.0 0.0 0.0 1.3105163696599734E-4 0.0 18-19 0.0 0.0 0.0 5.89732366346988E-4 0.0 20-21 0.0 0.0 0.0 0.0019002487360069613 0.0 22-23 0.0 0.0 0.0 0.0038004974720139226 0.0 24-25 0.0 0.0 0.0 0.006618107666782866 0.0 26-27 0.0 0.0 0.0 0.01336726697053173 0.0 28-29 0.0 0.0 0.0 0.03197659941970335 0.0 30-31 0.0 0.0 0.0 0.07646863016965945 0.0 32-33 0.0 0.0 0.0 0.15752406763312882 0.0 34-35 0.0 0.0 0.0 0.29984614537820187 0.0 36-37 0.0 0.0 0.0 0.49688228155657893 0.0 38-39 0.0 0.0 0.0 0.7572818842080157 0.0 40-41 0.0 0.0 0.0 1.0652532310781093 0.0 42-43 0.0 0.0 0.0 1.320476294069389 0.0 44-45 0.0 0.0 0.0 1.581006948357792 0.0 46-47 0.0 0.0 0.0 1.9169578197201262 0.0 48-49 0.0 0.0 0.0 2.3095229982517713 0.0 50-51 0.0 0.0 0.0 2.718207528130234 0.0 52-53 0.0 0.0 0.0 3.097274388054381 0.0 54-55 0.0 0.0 0.0 3.4735236377837597 0.0 56-57 0.0 0.0 0.0 3.8609778024737307 0.0 58-59 0.0 0.0 0.0 4.38977115763153 0.0 60-61 0.0 0.0 0.0 5.12529847010319 0.0 62-63 0.0 0.0 0.0 5.739013286014956 0.0 64-65 0.0 0.0 0.0 6.255094632387053 0.0 66-67 0.0 0.0 0.0 6.8171751033342165 0.0 68-69 0.0 0.0 0.0 7.449695829150602 0.0 70-71 0.0 0.0 0.0 8.220082877055217 0.0 72-73 0.0 0.0 0.0 9.034634326617374 0.0 74-75 0.0 0.0 0.0 9.809149501086416 0.0 76-77 0.0 0.0 0.0 10.510013655580572 0.0 78-79 0.0 0.0 0.0 11.267819746336452 0.0 80-81 0.0 0.0 0.0 12.06972471293139 0.0 82-83 0.0 0.0 0.0 12.961268999211068 0.0 84-85 0.0 0.0 0.0 14.112950784868254 0.0 86-87 0.0 0.0 0.0 15.341494355605995 0.0 88-89 0.0 0.0 0.0 16.301119967289512 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGGTC 45 1.52212215E-5 52.738827 8 CCGGTCC 45 1.52212215E-5 52.738827 9 CGCCGGT 55 4.9834E-5 43.14995 7 AGATAGT 65 1.333546E-4 36.511494 6 GGATCGT 65 1.333546E-4 36.511494 6 GTATAGG 210 0.0 34.001564 1 CTATCAC 155 3.6379788E-11 33.6848 2 GCTTATG 100 1.2768069E-6 33.32153 1 GCTATCA 180 5.456968E-12 31.734793 1 CGTCACT 180 5.456968E-12 31.734793 1 GTATGGT 60 0.0039087837 31.73479 1 GGGTACT 75 3.090842E-4 31.643293 7 CGATCCG 40 4.4655113E-4 29.720118 70-71 GTTCGAC 40 4.513798E-4 29.665588 16-17 ATGGGGT 475 0.0 28.978596 5 GGATAGC 85 6.432901E-4 27.920555 8 GTCACTA 225 3.6379788E-12 27.42419 2 CATATAG 70 0.008302331 27.20125 1 ATGGGTA 370 0.0 26.939562 5 GGACAAT 125 7.4291183E-6 26.58037 6 >>END_MODULE