##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939262_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 777902 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.986269478674693 33.0 31.0 34.0 30.0 34.0 2 31.93500852292448 33.0 31.0 34.0 30.0 34.0 3 31.962031207015794 33.0 31.0 34.0 30.0 34.0 4 35.48065051895997 37.0 35.0 37.0 33.0 37.0 5 35.56595817982214 37.0 35.0 37.0 33.0 37.0 6 35.751900625014464 37.0 35.0 37.0 35.0 37.0 7 35.702502371764055 37.0 35.0 37.0 35.0 37.0 8 35.72116153448635 37.0 35.0 37.0 35.0 37.0 9 37.28662350784546 39.0 37.0 39.0 34.0 39.0 10-11 37.22180043244522 39.0 37.0 39.0 34.0 39.0 12-13 37.1263809580127 39.0 37.0 39.0 33.5 39.0 14-15 38.09572542556775 40.0 38.0 41.0 33.0 41.0 16-17 38.09771025141984 40.0 38.0 41.0 33.0 41.0 18-19 38.26144552912835 40.0 38.0 41.0 34.0 41.0 20-21 38.24825235055316 40.0 38.0 41.0 34.0 41.0 22-23 38.176421322994415 40.0 38.0 41.0 34.0 41.0 24-25 38.14642268563392 40.0 38.0 41.0 34.0 41.0 26-27 37.885869299731844 40.0 37.5 41.0 33.5 41.0 28-29 37.64868582417837 40.0 37.0 41.0 33.0 41.0 30-31 37.33521189044379 39.0 36.0 41.0 33.0 41.0 32-33 36.911705459042395 39.0 35.0 41.0 32.5 41.0 34-35 36.55679378636383 39.0 35.0 41.0 31.5 41.0 36-37 36.17133135022149 39.0 35.0 41.0 31.0 41.0 38-39 35.65459530892066 38.0 35.0 40.5 29.5 41.0 40-41 35.32978639982928 38.0 35.0 40.0 29.5 41.0 42-43 35.03938799488881 38.0 35.0 40.0 26.5 41.0 44-45 34.56465210270703 38.0 35.0 40.5 23.5 41.0 46-47 33.87445205180087 38.0 34.5 41.0 13.5 41.0 48-49 33.375937457417514 38.0 34.0 41.0 2.0 41.0 50-51 32.118469935801684 37.0 32.0 39.0 2.0 40.5 52-53 32.08463662517901 37.5 32.5 39.5 2.0 40.5 54-55 32.50689547012349 38.0 33.0 40.0 2.0 41.0 56-57 32.473393820815474 38.0 33.0 40.0 2.0 41.0 58-59 32.29839877002502 38.0 33.0 40.0 2.0 41.0 60-61 31.93195993839841 37.0 33.0 40.0 2.0 41.0 62-63 31.42886700381282 36.0 32.0 40.0 2.0 41.0 64-65 30.915161549912455 36.0 31.0 39.0 2.0 41.0 66-67 30.497156454154894 35.0 31.0 39.0 2.0 40.5 68-69 30.10311838766323 35.0 30.5 38.5 2.0 40.0 70-71 29.571437019058955 35.0 29.5 37.5 2.0 39.5 72-73 29.009357219804038 35.0 29.0 37.0 2.0 39.0 74-75 28.54664790680574 35.0 28.5 36.0 2.0 39.0 76-77 27.962121192643806 34.5 27.0 36.0 2.0 37.5 78-79 27.49705811271857 34.0 26.0 35.0 2.0 37.0 80-81 27.082583667351415 34.0 25.5 35.0 2.0 36.5 82-83 26.619373005854207 34.0 24.5 35.0 2.0 36.0 84-85 26.167199724386876 34.0 22.0 35.0 2.0 36.0 86-87 25.740644065704934 34.0 19.0 35.0 2.0 35.0 88-89 25.18550601489648 33.0 11.0 35.0 2.0 35.0 90-91 24.64590334000941 33.0 2.0 35.0 2.0 35.0 92-93 24.20573928335446 33.0 2.0 35.0 2.0 35.0 94-95 23.740136289661166 32.5 2.0 35.0 2.0 35.0 96-97 23.07995223048662 32.0 2.0 35.0 2.0 35.0 98-99 22.34934220505925 31.5 2.0 35.0 2.0 35.0 100-101 20.30058413527668 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.412192970016406 1101 2 0.24534747267523827 1101 3 0.5433984787831783 1101 4 0.5619434265666214 1101 5 0.5745968511844168 1101 6 0.44311814537319094 1101 7 0.39134423794251916 1101 8 0.3497449209215304 1101 9 0.5181509893667027 1101 10-11 0.6711620911932243 1101 12-13 0.6445837446917082 1101 14-15 0.654954454059073 1101 16-17 0.633070728509658 1101 18-19 0.6089938719530394 1101 20-21 0.7157217980431128 1101 22-23 0.8515070478988562 1101 24-25 0.8484843842911118 1101 26-27 0.7777361623399273 1101 28-29 0.7746695911785721 1101 30-31 0.7685812981485327 1101 32-33 0.6307333011233709 1101 34-35 0.6372587441373696 1101 36-37 0.8241723202382829 1101 38-39 0.9131156934692299 1101 40-41 0.8440277805609 1101 42-43 0.8176799096232372 1101 44-45 0.8034274217122075 1101 46-47 0.5320646219674856 1101 48-49 0.4559773826779221 1101 50-51 0.4876878442320063 1101 52-53 0.8826520286896411 1101 54-55 0.6919574643878761 1101 56-57 0.4681599551432285 1101 58-59 0.4120259146154339 1101 60-61 0.7757034631829463 1101 62-63 0.6560168649038047 1101 64-65 0.5090694540848304 1101 66-67 0.6977411819288228 1101 68-69 0.8011801902956961 1101 70-71 0.6764764689256673 1101 72-73 0.5604367709614628 1101 74-75 0.5567486735772427 1101 76-77 0.37718206233832774 1101 78-79 0.10201260842354998 1101 80-81 -0.0802445752237233 1101 82-83 0.1296928409567002 1101 84-85 0.18902580841446337 1101 86-87 -0.11466106309845969 1101 88-89 -0.4300490544759157 1101 90-91 -0.20776112293108895 1101 92-93 -0.04164249939104181 1101 94-95 0.11396965593095842 1101 96-97 0.061294228369245474 1101 98-99 0.2854283094107579 1101 100-101 0.01750445324987382 1102 1 0.12592871860785237 1102 2 0.2045811471283976 1102 3 0.19852765912265014 1102 4 0.2468712076366799 1102 5 0.11846332797971826 1102 6 0.1869222653459488 1102 7 0.2686649289091818 1102 8 0.28497121869526154 1102 9 0.3196351701926403 1102 10-11 0.2606065405515281 1102 12-13 0.2114481694610788 1102 14-15 0.373366964341308 1102 16-17 0.3102359696669268 1102 18-19 0.35465653481048776 1102 20-21 0.4177181008584512 1102 22-23 0.43550633145876816 1102 24-25 0.40060871760380934 1102 26-27 0.36715960496722033 1102 28-29 0.42133858701260607 1102 30-31 0.467798078767494 1102 32-33 0.37800574294578126 1102 34-35 0.44186076609050673 1102 36-37 0.562385332913081 1102 38-39 0.7065244446522385 1102 40-41 0.7420832206408576 1102 42-43 0.8491612600685841 1102 44-45 0.9236699499496837 1102 46-47 0.751587172140411 1102 48-49 0.5920998939331028 1102 50-51 0.5514034705863935 1102 52-53 0.6890882699442145 1102 54-55 0.69019068774913 1102 56-57 0.7775404555899073 1102 58-59 0.7371238723189393 1102 60-61 0.6192154790330768 1102 62-63 0.533066679602328 1102 64-65 0.637951276849936 1102 66-67 0.6431789622028425 1102 68-69 0.47894578171256796 1102 70-71 0.43390842922957873 1102 72-73 0.8640536877924987 1102 74-75 0.8621412119863905 1102 76-77 0.8621766227006162 1102 78-79 0.6174658231167598 1102 80-81 0.6245345666402606 1102 82-83 0.39497026247997624 1102 84-85 0.43543697426591876 1102 86-87 0.413007012861879 1102 88-89 0.3053778022979792 1102 90-91 0.40364641649523136 1102 92-93 0.649577350421886 1102 94-95 0.45277726965476717 1102 96-97 0.30537917516693724 1102 98-99 0.41914499715786135 1102 100-101 0.14408769739673488 1103 1 0.3245404237689584 1103 2 0.35841633389771843 1103 3 0.3001993158092766 1103 4 0.3585139934935384 1103 5 0.352459621107748 1103 6 0.29435917723645133 1103 7 0.2716767623850913 1103 8 0.35332558139842973 1103 9 0.21176163155562477 1103 10-11 0.469648278504188 1103 12-13 0.4496146935557448 1103 14-15 0.6956286908882205 1103 16-17 0.6059926705508687 1103 18-19 0.5145020224067238 1103 20-21 0.4537620843200969 1103 22-23 0.40111974748385393 1103 24-25 0.21779577206631018 1103 26-27 0.3253013571770893 1103 28-29 0.559949757528706 1103 30-31 0.39298342236601513 1103 32-33 0.46537119062185894 1103 34-35 0.540676527424985 1103 36-37 0.5348422048834252 1103 38-39 0.9325411612191061 1103 40-41 0.8649446406579671 1103 42-43 0.4850323749816141 1103 44-45 0.5320335798327704 1103 46-47 0.3040893107203928 1103 48-49 0.11729503935654861 1103 50-51 0.35653873577030737 1103 52-53 0.39992997938247754 1103 54-55 0.28688123522560005 1103 56-57 0.3373449537364692 1103 58-59 0.4164616253842368 1103 60-61 0.2717897946874892 1103 62-63 0.029172681883480323 1103 64-65 0.10215233901132947 1103 66-67 0.36056561746374527 1103 68-69 0.0695725638625504 1103 70-71 -0.15961434664724194 1103 72-73 0.029530769343679708 1103 74-75 -0.0580808439018341 1103 76-77 -0.22634276709122858 1103 78-79 -0.33513789801240534 1103 80-81 -0.3195861205567745 1103 82-83 -0.16122538194545655 1103 84-85 -0.08775770779852365 1103 86-87 -0.1417094470753888 1103 88-89 -0.23059043978927107 1103 90-91 -0.23014849368441048 1103 92-93 -0.01937492360150017 1103 94-95 0.03636010398610168 1103 96-97 -0.17552063236086823 1103 98-99 -0.5073769589299637 1103 100-101 -0.7946999059679634 1104 1 0.2459894755712284 1104 2 0.2392292740078794 1104 3 0.08207462083611361 1104 4 0.29495293583475046 1104 5 0.21022124698796318 1104 6 0.1104083824236497 1104 7 0.2263367010311228 1104 8 0.2187237721535027 1104 9 0.37477233364026574 1104 10-11 0.26633154734613385 1104 12-13 0.367457892328666 1104 14-15 0.4293326318350168 1104 16-17 0.37528997585659596 1104 18-19 0.3800015256487299 1104 20-21 0.3980707191942585 1104 22-23 0.27477545061430675 1104 24-25 0.323282769207772 1104 26-27 0.3799959381573572 1104 28-29 0.3476565221525689 1104 30-31 0.5625522015614379 1104 32-33 0.6110378485734671 1104 34-35 0.35055874348856975 1104 36-37 0.36065226902621106 1104 38-39 0.35650559508928126 1104 40-41 0.4885850924463 1104 42-43 0.4694324307579478 1104 44-45 0.6891550850966581 1104 46-47 0.8464706117034453 1104 48-49 1.2110483075012866 1104 50-51 1.3492671137247072 1104 52-53 1.526674079291837 1104 54-55 1.6303959288043544 1104 56-57 1.6085541237148604 1104 58-59 1.5280186566887082 1104 60-61 1.4211734225896002 1104 62-63 1.339160567960306 1104 64-65 1.520691652613781 1104 66-67 1.4888076170454205 1104 68-69 1.3241207319584873 1104 70-71 1.2482533849988577 1104 72-73 1.3219632712609908 1104 74-75 1.5003775504368697 1104 76-77 1.4696892694422736 1104 78-79 1.6051138798583295 1104 80-81 1.4165416944052254 1104 82-83 1.2961591000318151 1104 84-85 1.3956117930032619 1104 86-87 1.3158101182177866 1104 88-89 0.9501216235865897 1104 90-91 0.9606915502394529 1104 92-93 1.0847754023414744 1104 94-95 1.019217595445351 1104 96-97 0.9243506027363928 1104 98-99 0.6875046906551994 1104 100-101 0.6257937581887667 1105 1 0.18553265123845364 1105 2 0.2720330202416754 1105 3 0.19465523867429013 1105 4 0.25768016822789974 1105 5 0.25077721260715435 1105 6 0.11511134173861137 1105 7 0.2663884900513622 1105 8 0.26161710048540243 1105 9 0.4034922494226123 1105 10-11 0.3469050179768445 1105 12-13 0.3477355121311092 1105 14-15 0.377317740474993 1105 16-17 0.269055933045955 1105 18-19 0.21902902949054948 1105 20-21 0.18316241883406548 1105 22-23 0.35725168372943017 1105 24-25 0.29306141551376896 1105 26-27 0.4238169514313199 1105 28-29 0.4259904403307573 1105 30-31 0.5187705140621972 1105 32-33 0.37564888641092153 1105 34-35 0.4475229953509938 1105 36-37 0.4451260498263636 1105 38-39 0.759633103367868 1105 40-41 0.5687521967382523 1105 42-43 0.5053983726203342 1105 44-45 0.9676027455155705 1105 46-47 0.7737370871863476 1105 48-49 0.5342507162554071 1105 50-51 0.30724096914299537 1105 52-53 0.4390078522671672 1105 54-55 0.3756627188934516 1105 56-57 0.39322231501155613 1105 58-59 0.520136359740782 1105 60-61 0.47897323624592403 1105 62-63 0.3737097735796624 1105 64-65 0.17366084944029225 1105 66-67 0.01744283115914058 1105 68-69 0.15018182202862818 1105 70-71 -0.15429532854413353 1105 72-73 0.00924471070534949 1105 74-75 0.31201213130477967 1105 76-77 0.3022139035108964 1105 78-79 0.30834645854012876 1105 80-81 0.14208005417757974 1105 82-83 0.14934993289121934 1105 84-85 0.1799149604423249 1105 86-87 -0.019716383320965036 1105 88-89 0.13461395808558052 1105 90-91 0.18057809033762595 1105 92-93 0.1078818158711492 1105 94-95 0.17720032437177835 1105 96-97 0.16607101462851048 1105 98-99 0.18322221462375765 1105 100-101 -0.2946814585063322 1106 1 -0.007304814326282383 1106 2 0.19814625571719446 1106 3 0.13248674231311597 1106 4 0.08223507962134136 1106 5 0.07841587878041878 1106 6 0.20782956878520054 1106 7 0.1416814467554488 1106 8 -0.017791640931484665 1106 9 -0.019537231273083933 1106 10-11 0.07551403898748532 1106 12-13 0.1666060355470833 1106 14-15 0.2255147718470596 1106 16-17 0.1796887195267729 1106 18-19 0.07301324066607862 1106 20-21 0.19257815009512314 1106 22-23 0.23878410427135321 1106 24-25 0.3890175850791664 1106 26-27 0.43775552303610965 1106 28-29 0.4781984087531086 1106 30-31 0.6943903487729415 1106 32-33 0.6334199484560585 1106 34-35 0.7299846539998995 1106 36-37 0.5482673680287391 1106 38-39 0.6849651209658276 1106 40-41 0.899423221778342 1106 42-43 1.0082765437481669 1106 44-45 1.0409035966318925 1106 46-47 1.1764240655178284 1106 48-49 1.234314447187522 1106 50-51 1.3053084828143113 1106 52-53 1.0708075315566745 1106 54-55 1.0807426909340734 1106 56-57 1.2929797263022138 1106 58-59 1.3058256817258993 1106 60-61 1.366693724712679 1106 62-63 1.4441735702985454 1106 64-65 1.346083858362622 1106 66-67 1.3747883350446735 1106 68-69 1.4757852100472135 1106 70-71 1.5139748906080435 1106 72-73 1.4108112581334993 1106 74-75 1.1272849408109664 1106 76-77 1.0920046193531725 1106 78-79 1.1205766299968971 1106 80-81 1.173554911296943 1106 82-83 0.8006081048413876 1106 84-85 0.543818582826276 1106 86-87 0.5842781070371643 1106 88-89 0.43028436637471046 1106 90-91 0.6016442471568375 1106 92-93 0.5077934149007959 1106 94-95 0.45839764356095714 1106 96-97 0.553390614973889 1106 98-99 0.7958512183653816 1106 100-101 0.762573588572323 1107 1 0.15108444741868254 1107 2 0.21265091363565247 1107 3 0.1407753784490957 1107 4 0.08272500340085998 1107 5 0.2580284929517873 1107 6 0.1114912481856365 1107 7 0.13237107366664702 1107 8 0.12622227363236505 1107 9 0.17741759192635698 1107 10-11 0.1640144897537894 1107 12-13 0.07728327776795396 1107 14-15 0.262162527577253 1107 16-17 0.19719393293502208 1107 18-19 0.13151724292446687 1107 20-21 0.20565765798580316 1107 22-23 0.3466474719964836 1107 24-25 0.2613560149554175 1107 26-27 0.2843028352851107 1107 28-29 0.1034850323769021 1107 30-31 0.36302976304993706 1107 32-33 0.3050653820568172 1107 34-35 0.19508987833581415 1107 36-37 0.19134787762232008 1107 38-39 0.5264939017112766 1107 40-41 0.27776822646813315 1107 42-43 0.5449716527061241 1107 44-45 0.3935248709801016 1107 46-47 0.358523634785584 1107 48-49 0.1446829827889573 1107 50-51 -0.026811575830848255 1107 52-53 -0.003934074628389794 1107 54-55 0.2328603819178383 1107 56-57 0.2886223187093009 1107 58-59 0.3655308168191951 1107 60-61 0.12384815997991083 1107 62-63 0.2797139890103537 1107 64-65 0.2691995186214271 1107 66-67 0.13762697892108022 1107 68-69 0.10374182720547154 1107 70-71 -0.11063603888117868 1107 72-73 -0.12539392704493935 1107 74-75 -0.13902323763785063 1107 76-77 -0.2178056945440865 1107 78-79 -0.5275096621543085 1107 80-81 -0.3637475938709329 1107 82-83 -0.5541194775031428 1107 84-85 -0.6669743105470047 1107 86-87 -0.5619085921696687 1107 88-89 -0.5252063314479507 1107 90-91 -0.7371535272414569 1107 92-93 -0.4491697861774 1107 94-95 -0.38282334120405537 1107 96-97 -0.15843949621513076 1107 98-99 -0.051476511502666966 1107 100-101 -0.0940417484096372 1108 1 0.1254973105077184 1108 2 0.12060567178426496 1108 3 0.22060104223689692 1108 4 0.42184993544004357 1108 5 0.19876487627754358 1108 6 0.2110910592322739 1108 7 0.2255161666727048 1108 8 0.34685740647906016 1108 9 0.35425119710296116 1108 10-11 0.2851630053112473 1108 12-13 0.18706963569196233 1108 14-15 0.2913914730927303 1108 16-17 0.3876339625258325 1108 18-19 0.320916246462879 1108 20-21 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-1.5799008084782002 2316 60-61 -1.5813682740955848 2316 62-63 -1.841627582666785 2316 64-65 -1.8356618144278265 2316 66-67 -1.6620534301553 2316 68-69 -1.6617601080951196 2316 70-71 -1.5264628916207847 2316 72-73 -1.9196590454711355 2316 74-75 -1.829740896811888 2316 76-77 -1.63553588878435 2316 78-79 -1.6687688503933629 2316 80-81 -1.427555697276354 2316 82-83 -1.358728688823767 2316 84-85 -1.6885182898091067 2316 86-87 -1.3867656640066066 2316 88-89 -1.546281577355412 2316 90-91 -1.7269408217267355 2316 92-93 -2.0328173839189567 2316 94-95 -2.2447245520985497 2316 96-97 -1.8652858440010753 2316 98-99 -1.7070462710992729 2316 100-101 -1.6613831070261647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2844.0 3 589.0 4 502.0 5 523.0 6 636.0 7 815.0 8 992.0 9 1268.0 10 1889.0 11 2867.0 12 5046.0 13 8072.0 14 8228.0 15 10305.0 16 14123.0 17 20135.0 18 15173.0 19 8122.0 20 7247.0 21 7236.0 22 7712.0 23 8345.0 24 9162.0 25 9434.0 26 10790.0 27 12240.0 28 14291.0 29 16567.0 30 19130.0 31 23061.0 32 27311.0 33 34357.0 34 45619.0 35 74502.0 36 93912.0 37 131484.0 38 108079.0 39 15290.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.74194130576307 17.555803355613854 13.445917637276578 17.2563377013465 2 13.258348883598222 24.20835210089441 40.20848079274178 22.32481822276559 3 23.43058807176146 32.991275002799355 28.895069649391285 14.683067276047895 4 11.4577367169873 16.45972525937929 38.13457991559313 33.947958108040275 5 10.87189335859665 43.14595229146179 30.770616977566796 15.211537372374769 6 24.969226910536165 35.8126732393098 19.758445717813665 19.45965413234036 7 24.222295399117076 39.63360671763895 19.690487461024727 16.45361042221925 8 25.490201122995682 29.55601150009326 21.32019527003994 23.633592106871113 9 30.187997247252074 18.899029463407103 21.241566493655174 29.671406795685645 10-11 23.257993168512385 27.81242543536102 25.065488253275696 23.864093142850894 12-13 31.629202281950846 25.84528013069121 25.361105215428253 17.16441237192969 14-15 27.38593828383263 24.997363106646258 30.507055297583065 17.109643311938054 16-17 18.263381779966785 29.639679503588155 32.23525537708752 19.86168333935754 18-19 17.829569047355655 29.330815519543503 36.708863201928494 16.13075223117235 20-21 20.131585040804357 27.17663963528213 33.80882670755678 18.88294861635673 22-23 18.51501873160289 27.525485786624397 32.08108622712583 21.878409254646876 24-25 19.41307367233281 27.788388979418272 34.06390866918376 18.734628679065153 26-27 21.615635262973363 24.799107071057975 35.54358979307594 18.041667872892724 28-29 18.619543737584785 25.599082431889997 38.02613499521403 17.75523883531119 30-31 18.135650567472783 30.103138275016533 35.60103742927495 16.160173728235726 32-33 16.723300112237776 29.726159120364564 34.54402197159001 19.006518795807644 34-35 17.73619330722815 27.384095421799763 34.74437920295429 20.135332068017796 36-37 20.350875835003986 27.42115678852758 34.891426905382986 17.336540471085446 38-39 22.101782223933274 25.195475858368756 35.57638051902911 17.126361398668863 40-41 18.0720265900687 27.702480084850944 37.01906244862037 17.20643087645999 42-43 17.31604383811832 30.182946884266492 34.523614516572145 17.977394761043044 44-45 18.094216550879867 30.58119577633486 31.81751630378346 19.507071369001817 46-47 17.573725910354298 29.699226538296063 30.507842660434765 22.219204890914874 48-49 19.17572007408301 29.27407810712878 30.91507741493904 20.63512440384917 50-51 20.925397703889765 30.438490228179017 30.336959789043743 18.299152278887473 52-53 17.85548414064294 30.71274863699579 31.690396142046584 19.741371080314686 54-55 16.173533127931794 30.253105693217297 32.34790471724825 21.22545646160266 56-57 19.085872433280112 31.950874767541134 28.199678619974566 20.763574179204184 58-59 18.059623643400606 29.43316985046701 30.16075134636425 22.346455159768126 60-61 18.660795245463515 28.207762211308008 31.183214248658082 21.948228294570395 62-63 22.05506317154633 27.733827587563418 30.230056512915166 19.981052727975086 64-65 20.332826294786795 28.709928795273832 32.161790452451264 18.79545445748811 66-67 18.582835348604362 29.19699035082683 32.26895884795698 19.95121545261183 68-69 18.33901640401955 31.381582979821605 31.074664903140476 19.204735713018373 70-71 18.159963610907695 31.11906136788146 29.020752750957314 21.70022227025353 72-73 18.334406339466454 29.621678250402216 28.489409493975472 23.55450591615586 74-75 19.045619168902014 29.97973026151962 29.81593374337298 21.158716826205378 76-77 19.937406551671202 27.993345898464966 31.686385150955736 20.382862398908095 78-79 18.401112287657398 27.990079178269117 33.22179072637904 20.38701780769444 80-81 17.414881494765883 31.656016575836194 31.24697312359458 19.682128805803348 82-83 17.18813891422151 30.72618708246855 29.444714611327317 22.640959391982623 84-85 18.294407141521056 28.553248579371378 29.36028173919774 23.79206253990983 86-87 19.75738781701923 28.242493508369787 30.372573781424585 21.6275448931864 88-89 20.881194294807827 26.10787898882558 31.59710418471498 21.41382253165161 90-91 18.938440792572376 27.40123492634961 32.43643097252063 21.223893308557383 92-93 17.963762019540948 29.739363706276745 31.152159775377168 21.144714498805143 94-95 18.102086176139462 30.108163732729448 29.90177197549622 21.88797811563487 96-97 18.744265751681723 28.407482298835152 28.39915917047444 24.449092779008684 98-99 20.123557384314946 27.59299867294518 28.753220059494893 23.53022388324498 100-101 21.660297280997945 27.39300238327899 29.867484528036588 21.079215807686477 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4414.0 1 2879.5 2 2225.5 3 2973.0 4 2215.0 5 2021.5 6 3834.0 7 7268.5 8 10050.5 9 9537.5 10 6906.0 11 5114.0 12 4718.0 13 4498.0 14 4170.0 15 4003.0 16 3763.0 17 3557.0 18 3539.5 19 3613.0 20 3761.0 21 3951.5 22 4206.5 23 4452.5 24 4732.0 25 5345.5 26 6128.0 27 6975.5 28 7628.0 29 8391.5 30 9605.5 31 10339.5 32 11225.0 33 12610.0 34 13967.0 35 14912.0 36 16315.0 37 18162.5 38 18197.0 39 17158.5 40 16293.0 41 15515.0 42 14694.5 43 14842.5 44 16975.0 45 18111.5 46 21647.5 47 32353.0 48 76868.5 49 80050.0 50 45890.0 51 34005.5 52 16799.0 53 10867.5 54 9242.0 55 8167.5 56 7719.5 57 7252.5 58 6471.5 59 6233.0 60 6259.0 61 6215.5 62 5274.0 63 3042.5 64 1507.5 65 1290.0 66 1217.0 67 944.0 68 733.5 69 598.5 70 531.5 71 466.0 72 401.0 73 531.5 74 524.5 75 312.5 76 198.5 77 165.0 78 145.0 79 104.0 80 78.5 81 57.5 82 45.0 83 37.0 84 28.0 85 15.0 86 7.0 87 5.5 88 4.0 89 2.0 90 1.0 91 1.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28114081208172753 2 0.10952536437751799 3 0.12070929243015187 4 0.1222519031960324 5 0.13086481330553207 6 0.056176742057482826 7 0.06247573601816167 8 0.06646081383001971 9 0.0638897958868855 10-11 0.060740298906546066 12-13 0.0638897958868855 14-15 0.06067602345796771 16-17 0.06209008332669154 18-19 0.06530385575560932 20-21 0.06864617908168381 22-23 0.07790184367696702 24-25 0.08786453820661214 26-27 0.08799308910376885 28-29 0.0809870652087281 30-31 0.07661633470539991 32-33 0.06871045453026217 34-35 0.2328056747508041 36-37 0.3542862725638962 38-39 0.3278690631981921 40-41 0.31167165015644643 42-43 0.33358957812166573 44-45 0.34689459597738537 46-47 0.35357924262953433 48-49 0.36322055991628766 50-51 0.35544323063830663 52-53 0.35685729050703047 54-55 0.34355227265131083 56-57 0.3208630393031513 58-59 0.3069152669616481 60-61 0.26397926731130655 62-63 0.3332682008787739 64-65 0.3616136737018288 66-67 0.3678483922139293 68-69 0.37787536219215273 70-71 0.3785181166779363 72-73 0.36264208087908245 74-75 0.36701281138241065 76-77 0.3523580091065456 78-79 0.32947594941265096 80-81 0.3286403685811323 82-83 0.34207393733400865 84-85 0.3492727875747845 86-87 0.3436165480998892 88-89 0.3578214222357058 90-91 0.38012500289239515 92-93 0.3745973143146566 94-95 0.37530434424901854 96-97 0.37961079930376834 98-99 0.3699052065684366 100-101 0.34830865584610915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 777902.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.653349888382916 #Duplication Level Percentage of deduplicated Percentage of total 1 74.64886017916427 23.62886490020061 2 11.984691886109658 7.5871129115098555 3 4.712422451411865 4.474918700292327 4 2.4981611825820242 3.16300679959381 5 1.4960723508613396 2.3677850790074775 6 0.9099017585649294 1.7280863236746373 7 0.6567177396748687 1.4551121474285609 8 0.4663683318494553 1.1809695987913826 9 0.3512431201112459 1.0006219239091714 >10 1.9507837761688624 11.43276627845209 >50 0.17600567936975148 3.806665505964546 >100 0.10812358125422289 6.496137857012159 >500 0.019104542552437875 4.161264643219063 >1k 0.01625918515101096 10.998877868578985 >5k 0.003251837030202192 6.9357841771494275 >10k+ 0.00203239814387637 9.582025285215918 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 19777 2.5423510930682784 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 16597 2.133559240109937 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16326 2.098721946980468 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 11424 1.4685654491182694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10349 1.3303732346748047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8645 1.1113225059197687 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7528 0.9677311537957223 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 7157 0.9200387709505825 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6819 0.876588567711614 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6667 0.857048831343794 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6342 0.8152697897678628 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5398 0.6939177428519273 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5350 0.6877472997884052 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4692 0.6031608094592893 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3491 0.448771181974079 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3442 0.44247218801340016 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 3370 0.4332165234181169 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3308 0.4252463677944008 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3218 0.41367678705029676 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3173 0.4078919966782448 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3151 0.4050638769407972 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3151 0.4050638769407972 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3067 0.3942656015796334 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2739 0.35210090731223215 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2727 0.3505582965463516 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2685 0.3451591588657697 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2681 0.3446449552771429 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2516 0.32343405724628554 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 2224 0.28589719527652585 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2220 0.285382991687899 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2115 0.27188514748644427 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1897 0.24386105190628127 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1878 0.24141858486030374 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1874 0.2409043812716769 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1763 0.22663523168728195 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1762 0.22650668079012526 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1749 0.224835519127088 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1716 0.2205933395209165 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1583 0.20349607019907393 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1515 0.19475460919241755 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1439 0.1849847410085075 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1350 0.1735437111615602 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1349 0.17341516026440348 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 1292 0.16608775912647095 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1291 0.16595920822931423 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1287 0.16544500464068737 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1165 0.1497617951875686 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1125 0.14461975930130017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1122 0.14423410660983002 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1121 0.1441055557126733 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1094 0.1406346814894421 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1090 0.14012047790081528 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1053 0.13536409470601696 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 975 0.1253371247277935 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 930 0.11955233435574147 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 922 0.11852392717848778 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 907 0.1165956637211371 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 876 0.11261058590927907 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 867 0.11145362783486865 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 860 0.11055377155477167 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.10656869374291364 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 814 0.10464043028556295 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 811 0.10425477759409282 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 804 0.10335492131399585 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 796 0.10232651413674217 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 781 0.10039825067939148 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.9282634573506688E-4 0.0 20-21 1.2855089715671126E-4 0.0 0.0 6.427544857835563E-4 0.0 22-23 1.2855089715671126E-4 0.0 0.0 0.002892395186026003 0.0 24-25 1.2855089715671126E-4 0.0 0.0 0.007777329277981031 0.0 26-27 1.2855089715671126E-4 0.0 0.0 0.013176466958562904 0.0 28-29 1.2855089715671126E-4 0.0 0.0 0.03020946083182715 0.0 30-31 1.2855089715671126E-4 0.0 0.0 0.07443096945373581 0.0 32-33 1.2855089715671126E-4 0.0 0.0 0.1491833161503634 0.0 34-35 1.2855089715671126E-4 0.0 0.0 0.2797267522130037 0.0 36-37 1.2855089715671126E-4 0.0 0.0 0.47094621173361173 0.0 38-39 1.2855089715671126E-4 0.0 0.0 0.7280480060470342 0.0 40-41 1.2855089715671126E-4 0.0 0.0 1.0336134885885369 0.0 42-43 1.2855089715671126E-4 0.0 0.0 1.3006779774316044 0.0 44-45 1.2855089715671126E-4 0.0 0.0 1.5698635560777578 0.0 46-47 1.2855089715671126E-4 0.0 0.0 1.9040316132366288 0.0 48-49 1.2855089715671126E-4 0.0 0.0 2.285442125100591 0.0 50-51 1.2855089715671126E-4 0.0 0.0 2.6755298225226314 0.0 52-53 1.2855089715671126E-4 0.0 0.0 3.0498057595943964 0.0 54-55 1.2855089715671126E-4 0.0 0.0 3.4121907386791652 0.0 56-57 1.2855089715671126E-4 0.0 0.0 3.8015714061668437 0.0 58-59 1.2855089715671126E-4 0.0 0.0 4.33139392879823 0.0 60-61 1.2855089715671126E-4 0.0 0.0 5.045044234363711 0.0 62-63 1.2855089715671126E-4 0.0 0.0 5.644669894151191 0.0 64-65 1.2855089715671126E-4 0.0 0.0 6.14389730325928 0.0 66-67 2.571017943134225E-4 0.0 0.0 6.695702029304463 0.0 68-69 2.571017943134225E-4 0.0 0.0 7.3227733056349 0.0 70-71 2.571017943134225E-4 0.0 0.0 8.091571946080613 0.0 72-73 2.571017943134225E-4 0.0 0.0 8.894308023375695 0.0 74-75 2.571017943134225E-4 0.0 0.0 9.660214268635382 0.0 76-77 2.571017943134225E-4 0.0 0.0 10.353039328861476 0.0 78-79 2.571017943134225E-4 0.0 0.0 11.100241418584861 0.0 80-81 2.571017943134225E-4 0.0 0.0 11.921874477761982 0.0 82-83 2.571017943134225E-4 0.0 0.0 12.782458458777583 0.0 84-85 2.571017943134225E-4 0.0 0.0 13.931896305704317 0.0 86-87 2.571017943134225E-4 1.2855089715671126E-4 0.0 15.17048419980923 0.0 88-89 2.571017943134225E-4 1.2855089715671126E-4 0.0 16.123367724983353 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCAA 110 1.6589183E-9 38.76481 9 TCCCGGT 25 0.0016050321 38.05266 94-95 AGATAGT 105 4.762478E-8 36.096127 6 GTCACTA 200 0.0 33.180386 2 CGTCACT 175 3.6379788E-12 32.557705 1 GTACAAC 75 3.0851492E-4 31.653324 1 GTATGGT 75 3.0851492E-4 31.653324 1 CGACCGT 60 1.535227E-7 31.598333 18-19 GTTCGAC 60 1.5382648E-7 31.591219 16-17 TCGACCG 65 3.096029E-7 29.167694 18-19 GTAAGGT 100 5.0223072E-5 28.440332 4 GTATAGT 120 5.396374E-6 27.696657 1 GTATAGG 190 3.965397E-10 27.488413 1 TTCGCGA 35 0.00835961 27.164686 48-49 CTAGATA 145 8.0275277E-7 26.152029 4 CGATCCG 75 1.0513286E-6 25.358618 70-71 TAGATAG 150 1.0789026E-6 25.280296 5 TCACTAC 285 0.0 24.947662 3 CATGAGA 210 1.2951205E-9 24.828861 4 TGGGAGT 405 0.0 24.565418 6 >>END_MODULE