##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939260_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 791585 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.187358274853615 33.0 31.0 34.0 30.0 34.0 2 32.3850628801708 34.0 31.0 34.0 30.0 34.0 3 32.462769001433834 34.0 31.0 34.0 30.0 34.0 4 36.0284328278075 37.0 35.0 37.0 35.0 37.0 5 35.96763581927399 37.0 35.0 37.0 35.0 37.0 6 36.01851096218347 37.0 35.0 37.0 35.0 37.0 7 35.967698983684635 37.0 35.0 37.0 35.0 37.0 8 35.96359077041632 37.0 35.0 37.0 35.0 37.0 9 37.627832765906376 39.0 37.0 39.0 35.0 39.0 10-11 37.55982996140654 39.0 37.0 39.0 35.0 39.0 12-13 37.508260641624084 39.0 37.0 39.0 35.0 39.0 14-15 38.72350916199777 40.0 38.0 41.0 34.0 41.0 16-17 38.6909434868018 40.0 38.0 41.0 34.0 41.0 18-19 38.84472861410967 40.0 38.0 41.0 35.0 41.0 20-21 38.871509060934706 40.0 38.0 41.0 35.0 41.0 22-23 38.79920349678177 40.0 38.0 41.0 34.0 41.0 24-25 38.724190074344506 40.0 38.0 41.0 34.0 41.0 26-27 38.52856294649343 40.0 38.0 41.0 34.0 41.0 28-29 38.37389857058939 40.0 38.0 41.0 34.0 41.0 30-31 38.148297403311076 40.0 37.5 41.0 34.0 41.0 32-33 37.87702773549272 40.0 37.0 41.0 33.0 41.0 34-35 37.73646734084147 40.0 37.0 41.0 33.0 41.0 36-37 37.5848575958362 40.0 36.5 41.0 33.0 41.0 38-39 37.423560325170385 40.0 36.0 41.0 33.0 41.0 40-41 37.25138487970338 40.0 36.0 41.0 32.5 41.0 42-43 37.01377299974103 40.0 36.0 41.0 31.5 41.0 44-45 36.68071969529488 40.0 36.0 41.0 31.0 41.0 46-47 36.247660074407676 40.0 36.0 41.0 30.0 41.0 48-49 35.8331082574834 40.0 35.0 41.0 28.5 41.0 50-51 35.5838286475868 39.0 35.0 41.0 27.0 41.0 52-53 35.397154443300465 39.0 35.0 41.0 26.5 41.0 54-55 35.16355729327867 39.0 35.0 41.0 26.0 41.0 56-57 34.939974228920455 39.0 35.0 40.0 24.5 41.0 58-59 34.684479872660546 39.0 35.0 40.0 24.0 41.0 60-61 34.346933683685265 38.0 34.0 40.0 23.0 41.0 62-63 34.001866508334544 38.0 34.0 40.0 22.0 41.0 64-65 33.599762501815974 37.5 33.5 40.0 20.5 41.0 66-67 33.21569761933336 37.0 33.0 39.5 18.5 41.0 68-69 32.81490932748852 36.5 33.0 39.0 15.5 40.0 70-71 32.424102275813716 36.0 32.5 39.0 12.0 40.0 72-73 31.91957338757051 35.5 32.0 38.5 7.5 40.0 74-75 31.414188621563067 35.0 31.0 37.5 4.5 39.5 76-77 29.364981650738706 33.5 28.5 35.5 2.0 38.0 78-79 30.29049122962158 34.5 30.5 36.0 2.0 39.0 80-81 30.28019858890707 35.0 31.0 36.0 2.0 38.0 82-83 29.928772652336768 34.0 30.5 36.0 2.0 37.0 84-85 29.521933841596294 34.0 30.0 35.0 2.0 37.0 86-87 29.00334708212005 34.0 29.5 35.0 2.0 36.0 88-89 28.466463487812426 34.0 29.0 35.0 2.0 36.0 90-91 28.02616269888894 34.0 28.5 35.0 2.0 35.5 92-93 27.689540605241383 34.0 27.5 35.0 2.0 35.0 94-95 27.29346753665115 33.5 27.0 35.0 2.0 35.0 96-97 26.74694000012633 33.0 25.5 35.0 2.0 35.0 98-99 26.05855340866742 33.0 24.5 35.0 2.0 35.0 100-101 22.92044379314919 29.0 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17029165731700147 1101 2 0.1596100589426328 1101 3 0.15239181419983083 1101 4 0.19076426747606945 1101 5 0.09187478701995389 1101 6 0.1502970097422036 1101 7 0.13920840712685845 1101 8 0.14523691947085382 1101 9 0.24852862886869787 1101 10-11 0.2206765739859904 1101 12-13 0.10714613840588072 1101 14-15 0.1698511100383655 1101 16-17 0.09037408294322091 1101 18-19 0.08870399722115252 1101 20-21 0.3237372557517446 1101 22-23 0.2590677955798668 1101 24-25 0.4540949521700952 1101 26-27 0.20157905303591406 1101 28-29 0.09220436982558056 1101 30-31 0.2916124788684442 1101 32-33 0.1948639026695247 1101 34-35 0.27572584079343443 1101 36-37 0.25827884915737087 1101 38-39 0.2878240055204131 1101 40-41 0.3571772579136976 1101 42-43 0.3814459592623507 1101 44-45 0.22611995266255747 1101 46-47 0.060033983194252016 1101 48-49 0.19776961647540503 1101 50-51 0.2627132049053742 1101 52-53 0.05282620785471437 1101 54-55 -0.15200413445096927 1101 56-57 -0.10883668599953467 1101 58-59 -0.12825401846131967 1101 60-61 0.04564776389332792 1101 62-63 -0.0722667143761413 1101 64-65 -0.12627929516369107 1101 66-67 -0.18580009588908553 1101 68-69 -0.06783607512122813 1101 70-71 -0.014613832515792069 1101 72-73 -0.2830783467626894 1101 74-75 -0.35407969981668685 1101 76-77 -0.09690900280119408 1101 78-79 0.09701481835598003 1101 80-81 0.05604262384566994 1101 82-83 0.003650195575140458 1101 84-85 -0.06771380305212205 1101 86-87 0.012260052195887994 1101 88-89 -0.05278022727310372 1101 90-91 0.22496795866276287 1101 92-93 0.06774854424351773 1101 94-95 0.23154768144617677 1101 96-97 0.10195677000797687 1101 98-99 -0.28072382478962155 1101 100-101 -0.4034857623788035 1102 1 0.25970337615837025 1102 2 0.21740507668073406 1102 3 0.18783552659571257 1102 4 0.1149257686521139 1102 5 0.1503065570614126 1102 6 0.22002138695946627 1102 7 0.23860024370243593 1102 8 0.24015849497798314 1102 9 0.24898994941500519 1102 10-11 0.2671432484496279 1102 12-13 0.27769829361750453 1102 14-15 0.3296402020815634 1102 16-17 0.3556593869599638 1102 18-19 0.3804145218270776 1102 20-21 0.286844606992112 1102 22-23 0.236719738973882 1102 24-25 0.3849293575863868 1102 26-27 0.49718481239551693 1102 28-29 0.375721581630188 1102 30-31 0.31972054483077983 1102 32-33 0.38396309308445353 1102 34-35 0.614110958982458 1102 36-37 0.5232880105935749 1102 38-39 0.4571968694763342 1102 40-41 0.5696218669607802 1102 42-43 0.5022989474499511 1102 44-45 0.527508462532218 1102 46-47 0.5888437761847811 1102 48-49 0.5617482787757737 1102 50-51 0.5563827174649916 1102 52-53 0.5603795196164398 1102 54-55 0.497739865133191 1102 56-57 0.5306932868410357 1102 58-59 0.299523326151629 1102 60-61 0.3235836468348623 1102 62-63 0.4087931533353242 1102 64-65 0.5902717164927722 1102 66-67 0.4387726952951496 1102 68-69 0.4177395359660565 1102 70-71 0.47692970704731863 1102 72-73 0.5532893062202966 1102 74-75 0.7145855893858162 1102 76-77 0.671306661305465 1102 78-79 0.6803189130631395 1102 80-81 0.5411601631228891 1102 82-83 0.795964985122005 1102 84-85 0.8757939224426607 1102 86-87 0.7338660974442881 1102 88-89 0.9791775911355352 1102 90-91 1.0711045615242476 1102 92-93 0.9953926906705597 1102 94-95 1.0088955431279807 1102 96-97 0.7434944618458559 1102 98-99 0.6077113239375223 1102 100-101 0.4712592118262293 1103 1 0.19686408418613865 1103 2 0.17463789466651036 1103 3 0.1903058408996685 1103 4 0.20822152052849674 1103 5 0.1792150393338403 1103 6 0.17099564538143852 1103 7 0.1691884967021764 1103 8 0.14729058691925445 1103 9 0.15705512787317133 1103 10-11 0.24128127215517736 1103 12-13 0.20511104250328316 1103 14-15 0.2826289813936995 1103 16-17 0.28671203324922345 1103 18-19 0.30064852444574797 1103 20-21 0.37785171693936803 1103 22-23 0.12749379228881708 1103 24-25 0.17737028181502978 1103 26-27 0.1237545584626858 1103 28-29 0.08799553927150328 1103 30-31 0.19020104138760985 1103 32-33 0.2192088237836458 1103 34-35 0.38685386784742093 1103 36-37 0.2967640588646745 1103 38-39 0.17275060472486103 1103 40-41 0.20704250623254694 1103 42-43 0.28261942181909205 1103 44-45 0.5195449776045749 1103 46-47 0.335337714347375 1103 48-49 0.45074538276761444 1103 50-51 0.5545358941515843 1103 52-53 0.6219911793279564 1103 54-55 0.5609672264978158 1103 56-57 0.662563781312393 1103 58-59 0.7827180939872349 1103 60-61 0.5982937772734829 1103 62-63 0.7610709379213318 1103 64-65 1.063255206881344 1103 66-67 0.839417255981239 1103 68-69 0.7808144645333357 1103 70-71 1.0811430011652163 1103 72-73 1.0021006958957237 1103 74-75 1.1863328476379316 1103 76-77 1.0320682233008966 1103 78-79 1.048527967442304 1103 80-81 0.8746493692038619 1103 82-83 0.7776042504346208 1103 84-85 0.958710699295235 1103 86-87 0.7851095319766586 1103 88-89 0.9507904763293418 1103 90-91 1.4170592874784624 1103 92-93 1.0519497734207128 1103 94-95 1.3300686521765535 1103 96-97 1.0677624511734116 1103 98-99 0.6795600607232757 1103 100-101 0.6067663787476683 1104 1 0.2843258553205672 1104 2 0.3144181494836644 1104 3 0.2764335347499127 1104 4 0.24333334389330474 1104 5 0.2589429379104047 1104 6 0.22271631141322956 1104 7 0.2708783279288127 1104 8 0.2678882367617206 1104 9 0.4234489659463989 1104 10-11 0.3987453926080917 1104 12-13 0.3344242444146204 1104 14-15 0.4371994546555129 1104 16-17 0.421169613367276 1104 18-19 0.3194095106560866 1104 20-21 0.3743686425205084 1104 22-23 0.2627065104922508 1104 24-25 0.4583760418405376 1104 26-27 0.3833605441905803 1104 28-29 0.5606878582586106 1104 30-31 0.4921052479262684 1104 32-33 0.33742883746003116 1104 34-35 0.5889088922425785 1104 36-37 0.5679372917366763 1104 38-39 0.5379951645842311 1104 40-41 0.7100882298436133 1104 42-43 0.8578154430804261 1104 44-45 0.7270328010029488 1104 46-47 0.43271277045832335 1104 48-49 0.31113608282257843 1104 50-51 0.2179561662530105 1104 52-53 0.27697907614253126 1104 54-55 0.5268465218700769 1104 56-57 0.4727766043961239 1104 58-59 0.4695623299762772 1104 60-61 0.48737972457323053 1104 62-63 0.5182468473383324 1104 64-65 0.4371995867661411 1104 66-67 0.5189188228927719 1104 68-69 0.6112660371084004 1104 70-71 0.38597022137606984 1104 72-73 0.5953015164791076 1104 74-75 0.46742041428935366 1104 76-77 0.4781136070998251 1104 78-79 0.6135390637769476 1104 80-81 0.7164059881989431 1104 82-83 0.7972640607271515 1104 84-85 0.5464577448174772 1104 86-87 0.698398703716034 1104 88-89 1.0614821828155634 1104 90-91 1.2046148215918215 1104 92-93 0.8741063638883553 1104 94-95 0.7138675840756861 1104 96-97 0.7217004962887152 1104 98-99 0.8739976296820657 1104 100-101 1.1781888071878477 1105 1 0.13942034769688405 1105 2 0.11102737727339473 1105 3 0.2045780553032941 1105 4 0.10284885715240222 1105 5 0.17490782373377556 1105 6 0.22011290952893603 1105 7 0.02063822760259626 1105 8 0.04515847463963496 1105 9 0.18011819897467518 1105 10-11 0.17489053226314866 1105 12-13 0.13042627633804216 1105 14-15 0.3708762042130189 1105 16-17 0.343379091972551 1105 18-19 0.3099940061279085 1105 20-21 0.20303027171036092 1105 22-23 0.2573885859115421 1105 24-25 0.2860534581229146 1105 26-27 0.42366168159146156 1105 28-29 0.328496952703361 1105 30-31 0.30418408020527465 1105 32-33 0.3599923152239697 1105 34-35 0.37285034744898127 1105 36-37 0.36272691622770026 1105 38-39 0.43375590560130917 1105 40-41 0.5202383223120037 1105 42-43 0.35362460102278703 1105 44-45 0.3993911021244756 1105 46-47 0.3828671490151834 1105 48-49 0.6916554138752886 1105 50-51 0.8287396473505666 1105 52-53 0.8791546629001132 1105 54-55 0.557335294699449 1105 56-57 0.5360737970081786 1105 58-59 0.8880988688804692 1105 60-61 0.6481735240319182 1105 62-63 0.5419889994309699 1105 64-65 0.7152417174283414 1105 66-67 0.6965632997915492 1105 68-69 0.689683597371598 1105 70-71 0.8337751884763662 1105 72-73 0.8694702931183365 1105 74-75 0.9416205428720126 1105 76-77 1.1269202489495136 1105 78-79 1.2048744184507676 1105 80-81 0.971352848639139 1105 82-83 1.0437796295466555 1105 84-85 0.7680505029154041 1105 86-87 0.8678480207199009 1105 88-89 0.7808227601675526 1105 90-91 0.7998235302886165 1105 92-93 0.9024342337817579 1105 94-95 0.7913128372327058 1105 96-97 0.6859989667901054 1105 98-99 0.9764649085714936 1105 100-101 1.0163472972147538 1106 1 0.1929892412288723 1106 2 0.21162398255657422 1106 3 0.09780791395989041 1106 4 0.13311228805138597 1106 5 0.12636402839231664 1106 6 0.1998068535667059 1106 7 0.09493542366696772 1106 8 0.22944726217316713 1106 9 0.39924050851781345 1106 10-11 0.3058433898896098 1106 12-13 0.32095230092298266 1106 14-15 0.500012673576478 1106 16-17 0.3180224574233179 1106 18-19 0.2901293070331903 1106 20-21 0.3700433504338676 1106 22-23 0.2767291168502055 1106 24-25 0.31592285289070077 1106 26-27 0.4142484951222798 1106 28-29 0.41688389800422954 1106 30-31 0.4025420506552919 1106 32-33 0.5559911649660663 1106 34-35 0.4568655847260743 1106 36-37 0.5993269577243439 1106 38-39 0.4446187446270784 1106 40-41 0.5621735948248485 1106 42-43 0.717614794710201 1106 44-45 0.869739200740888 1106 46-47 0.7041057874883947 1106 48-49 0.6154945088116008 1106 50-51 0.7634060907604194 1106 52-53 0.8648874609024446 1106 54-55 0.7255605122838418 1106 56-57 0.853560447100449 1106 58-59 0.7284077640868745 1106 60-61 0.6213244662605746 1106 62-63 0.518818218893621 1106 64-65 0.49028348707827973 1106 66-67 0.6018599462073624 1106 68-69 0.6361456443210471 1106 70-71 0.8358090701235739 1106 72-73 1.0125891300474343 1106 74-75 0.8133176464789464 1106 76-77 0.8027204244002419 1106 78-79 0.8905309572665132 1106 80-81 0.8419815956224426 1106 82-83 0.9331328278824174 1106 84-85 0.8387194725245735 1106 86-87 0.6227893976077254 1106 88-89 0.9173694748344339 1106 90-91 1.023119164768687 1106 92-93 1.1328953197562548 1106 94-95 1.1435201675787674 1106 96-97 1.2369260892161336 1106 98-99 1.4682993515154266 1106 100-101 1.6533299086641868 1107 1 0.11715833837319423 1107 2 0.08609662935095486 1107 3 0.08857238001107959 1107 4 0.006233763577640161 1107 5 0.09315659070897908 1107 6 0.06502203729057499 1107 7 0.08968761615741982 1107 8 0.14130062719689107 1107 9 -0.007470477466100078 1107 10-11 -0.014015622726383015 1107 12-13 0.20352944504285375 1107 14-15 0.12190654000114165 1107 16-17 0.055966079338269026 1107 18-19 0.031212167062975027 1107 20-21 -0.02735956681406293 1107 22-23 0.08272133369884926 1107 24-25 -0.014145852734465336 1107 26-27 0.12030380036721766 1107 28-29 0.0710650224283853 1107 30-31 0.12250716648322424 1107 32-33 0.02410284829606013 1107 34-35 -0.03879413603186066 1107 36-37 0.08737389953964225 1107 38-39 0.3133417443959843 1107 40-41 0.2320821166502256 1107 42-43 0.2622287493911415 1107 44-45 0.03455088862538247 1107 46-47 -0.15902191253470477 1107 48-49 0.01690370790947071 1107 50-51 -0.18855648128938896 1107 52-53 -0.204758586204683 1107 54-55 -0.31993651125960554 1107 56-57 -0.025391644516048473 1107 58-59 0.013360675515151854 1107 60-61 -0.17869628206226196 1107 62-63 -0.03256677382759676 1107 64-65 -0.02867761234667654 1107 66-67 0.010494790907010554 1107 68-69 0.2810625582526214 1107 70-71 0.11134089349630472 1107 72-73 0.21166001002935175 1107 74-75 0.1272498922402967 1107 76-77 0.07645068608213634 1107 78-79 0.12685604131408823 1107 80-81 0.2451181347684539 1107 82-83 0.3088292883534898 1107 84-85 0.14598104814883683 1107 86-87 0.2684797347932708 1107 88-89 0.3680972686467072 1107 90-91 0.2502660679549855 1107 92-93 0.2570048709008681 1107 94-95 0.5322593948977286 1107 96-97 0.7059807247794332 1107 98-99 0.8587532674661631 1107 100-101 0.9879708361648234 1108 1 0.0578171264043732 1108 2 0.15107221263907178 1108 3 0.14098394387170288 1108 4 0.06820853959236217 1108 5 0.15141923247809785 1108 6 0.07566878239068586 1108 7 0.055107199816092134 1108 8 0.014169954777855764 1108 9 0.07618653721038982 1108 10-11 0.1607938078150184 1108 12-13 0.15281183192532666 1108 14-15 0.2727310175893791 1108 16-17 0.26890098134057183 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-1.3402381745168874 2316 78-79 -1.1468284355394154 2316 80-81 -1.2766328066981565 2316 82-83 -1.2019230060552388 2316 84-85 -1.2541848736894607 2316 86-87 -1.4402561964730047 2316 88-89 -1.6958312438094119 2316 90-91 -1.985045321793546 2316 92-93 -1.5990772430978204 2316 94-95 -1.4360063889830847 2316 96-97 -1.0869332408856671 2316 98-99 -1.1303226570886693 2316 100-101 -0.3915287616811227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 17.0 6 73.0 7 156.0 8 355.0 9 635.0 10 1109.0 11 1767.0 12 2520.0 13 3793.0 14 4350.0 15 6058.0 16 8198.0 17 11467.0 18 10279.0 19 5740.0 20 4923.0 21 5083.0 22 5348.0 23 5763.0 24 6345.0 25 7294.0 26 8295.0 27 10046.0 28 11650.0 29 14052.0 30 17444.0 31 20852.0 32 25639.0 33 32408.0 34 42956.0 35 61809.0 36 90796.0 37 167475.0 38 178758.0 39 18116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.11385314616221 23.368649596295086 16.018990725047438 20.49850653249527 2 14.255070523064484 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19.001939147406784 29.42880423454209 32.962474023636126 18.606782594415 22-23 18.651818819204507 29.501316977961938 32.7571896890416 19.089674513791948 24-25 19.02364058876922 30.009847350279532 32.873916649349056 18.09259541160219 26-27 18.80265596398076 29.256913730439365 34.24499448569122 17.69543581988865 28-29 17.692919901210864 30.3247282351232 33.97796825356721 18.004383610098724 30-31 18.472116070921 29.7403311078406 33.34146048750292 18.446092333735482 32-33 18.490623243239828 29.99223077749073 33.253030312600664 18.264115666668772 34-35 19.06134128392847 29.49201255756075 33.175332099475085 18.271314059035696 36-37 19.109011039276524 29.097430318239226 33.30054340306406 18.493015239420192 38-39 18.39948201257067 30.065253782255773 33.15239569186065 18.382868513312907 40-41 18.26199520993947 29.91545056704284 33.647047971995015 18.175506251022675 42-43 18.057268110266065 30.039290497005837 32.58672966622028 19.316711726507823 44-45 18.522867527138725 29.55073454264314 32.38904167735203 19.5373562528661 46-47 19.415753729231515 29.98298348627562 31.440819925617497 19.160442858875367 48-49 18.987979812654356 29.97359727635061 31.55163374748132 19.48678916351371 50-51 18.229627898456894 30.1798290771048 31.991068552334873 19.599474472103438 52-53 18.5052142220987 31.101397828407563 31.213198835248267 19.18018911424547 54-55 18.430932875180808 30.107821648970106 31.2996077490099 20.161637726839192 56-57 19.020762141778835 30.422506742800838 30.711104935035404 19.845626180384922 58-59 19.127835145316592 29.773226273462512 31.179145053828915 19.91979352739198 60-61 18.795400509875364 30.46669513344434 31.335858285979945 19.402046070700358 62-63 18.4938489642156 30.24347692066692 31.470083656107956 19.792590459009517 64-65 18.31483668841628 30.627538419752774 31.115546656391924 19.942078235439023 66-67 18.46207292962853 30.17357580045099 30.323338618089025 21.041012651831455 68-69 18.037234297899648 30.064667968700654 30.842098481269154 21.055999252130544 70-71 18.792170139656513 30.057479613686468 30.71243138765894 20.437918858998085 72-73 18.69552859137048 29.03522679181642 31.280721590227202 20.988523026585902 74-75 18.05384134363334 30.61888489549448 30.128981726536004 21.198292034336173 76-77 18.291049245546528 29.639948998261534 30.63320405633489 21.435797699857048 78-79 18.176317135872964 29.442258254009364 30.90678827921196 21.474636330905714 80-81 18.47310741243723 29.57287685942583 30.264346398003976 21.68966933013296 82-83 18.21219452111902 29.56258645628707 30.6454771123758 21.579741910218107 84-85 18.00741550180966 29.176209756374867 30.414611191470275 22.401763550345194 86-87 17.89331050303378 29.43376135929648 30.501971516081955 22.170956621587784 88-89 18.299184068803942 29.132585016075215 30.077067211635004 22.491163703485835 90-91 18.135003717670056 28.65407666450516 30.52514545819887 22.68577415962591 92-93 18.417605184534825 29.144374893410056 30.362121566224726 22.075898355830393 94-95 18.098435417548338 28.612972706658162 30.350309821434212 22.93828205435929 96-97 17.826702122955844 28.318437059823015 30.473606751012213 23.381254066208935 98-99 18.089781893290045 28.53559630361869 29.616781520620023 23.757840282471243 100-101 17.798397513840943 28.20552500844827 30.500800611433426 23.49527686627736 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1995.0 1 1272.5 2 590.5 3 629.0 4 520.5 5 516.5 6 963.0 7 1781.5 8 2488.5 9 2480.5 10 1938.5 11 1549.0 12 1483.5 13 1563.5 14 1650.5 15 1656.0 16 1645.5 17 1752.5 18 1917.0 19 2117.0 20 2411.5 21 2744.0 22 3051.0 23 3490.0 24 3978.0 25 4921.5 26 7000.5 27 9500.0 28 10786.0 29 12082.5 30 14916.0 31 17777.5 32 21484.0 33 25978.0 34 30469.0 35 33504.5 36 35957.5 37 39389.0 38 38869.5 39 35960.0 40 33923.5 41 31640.5 42 29693.0 43 29068.5 44 29301.0 45 28189.5 46 26361.5 47 28307.5 48 36363.5 49 34170.5 50 22748.0 51 16104.0 52 11614.5 53 9198.0 54 7850.5 55 6615.0 56 5432.0 57 4386.5 58 3530.0 59 2919.0 60 2386.5 61 1904.0 62 1472.5 63 1060.5 64 740.0 65 540.5 66 414.0 67 344.0 68 298.0 69 241.0 70 194.0 71 164.0 72 145.0 73 129.5 74 100.5 75 76.0 76 58.0 77 37.0 78 27.0 79 16.5 80 12.0 81 10.5 82 8.5 83 4.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27122797930733905 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022739187832007935 16-17 5.053152851557318E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.8949323193339946E-4 26-27 0.0010106305703114636 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0037898646386679887 36-37 0.023181338706519197 38-39 0.007579729277335977 40-41 0.019517802889140144 42-43 0.005684796958001983 44-45 5.053152851557318E-4 46-47 1.2632882128893295E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.789864638667989E-4 60-61 0.0024002476044897264 62-63 0.0013896170341782625 64-65 0.0 66-67 0.0 68-69 3.789864638667989E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2284025088903908 78-79 0.0 80-81 0.0012632882128893296 82-83 0.0 84-85 0.0 86-87 0.10396861992079183 88-89 0.35283639785998977 90-91 0.3517626028790338 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 3.158220532223324E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 791585.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.29669917558921 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98107037341292 28.332307967786896 2 13.29811550839017 10.18547858454112 3 4.744357360656098 5.45079679867617 4 2.283790645752567 3.498465733616427 5 1.290196443569015 2.470513253838881 6 0.7722437795166709 1.774463262862199 7 0.515549880706024 1.3820701083986653 8 0.39178204426903557 1.2003167273414903 9 0.29555415275678076 1.0186873630400317 >10 1.957004681506189 15.22007032433161 >50 0.26371774304293316 7.0158159541026235 >100 0.18714386278129885 13.288008053857265 >500 0.012542269463296538 3.1231400998306746 >1k 0.006271134731648269 4.530878941170088 >5k 6.6011944543666E-4 1.5089868266059008 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6325 0.799029794652501 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5613 0.7090836738947807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4552 0.5750487945072228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3467 0.43798202340873055 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3166 0.3999570482007618 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2558 0.3231491248570905 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2369 0.2992729776334822 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1781 0.2249916307155896 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1659 0.2095795145183398 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1574 0.19884156470878048 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1573 0.19871523588749154 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1572 0.19858890706620264 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1560 0.19707296121073545 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1428 0.18039755680059627 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1393 0.1759760480554836 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1324 0.16725935938654724 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1289 0.1628378506414346 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1236 0.15614242311312115 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1158 0.14628877505258436 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1118 0.14123562220102703 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1068 0.1349191811365804 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 969 0.12241262782897605 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 917 0.11584352912195153 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 881 0.11129569155554994 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 872 0.11015873216394954 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 863 0.10902177277234913 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 857 0.10826379984461554 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 851 0.10750582691688194 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.4215087451126536E-4 0.0 22-23 0.0 0.0 0.0 0.002273918783200793 0.0 24-25 0.0 0.0 0.0 0.005242646083490718 0.0 26-27 0.0 0.0 0.0 0.010358963345692503 0.0 28-29 0.0 0.0 0.0 0.023244503117163667 0.0 30-31 0.0 0.0 0.0 0.06221694448479949 0.0 32-33 0.0 0.0 0.0 0.13371905733433553 0.0 34-35 0.0 0.0 0.0 0.24071956896606178 0.0 36-37 0.0 0.0 0.0 0.4054523519268304 0.0 38-39 0.0 0.0 0.0 0.6301913249998421 0.0 40-41 0.0 0.0 0.0 0.8645944529014572 0.0 42-43 0.0 0.0 0.0 1.1015873216394954 0.0 44-45 0.0 0.0 0.0 1.3851323610225055 0.0 46-47 0.0 0.0 0.0 1.7149769134079094 0.0 48-49 0.0 0.0 0.0 2.04816917955747 0.0 50-51 0.0 0.0 0.0 2.3937416701933465 0.0 52-53 0.0 0.0 0.0 2.726175963415173 0.0 54-55 0.0 0.0 0.0 3.0985301641643033 0.0 56-57 0.0 0.0 0.0 3.5553983463557293 0.0 58-59 0.0 0.0 0.0 4.026162698888938 0.0 60-61 0.0 0.0 0.0 4.569818781305861 0.0 62-63 0.0 0.0 0.0 5.0741866003019265 0.0 64-65 0.0 0.0 0.0 5.606788910856068 0.0 66-67 0.0 0.0 0.0 6.214872692130346 0.0 68-69 0.0 0.0 0.0 6.899448574695073 0.0 70-71 0.0 0.0 0.0 7.647125703493623 0.0 72-73 0.0 0.0 0.0 8.387286267425482 0.0 74-75 0.0 0.0 0.0 9.175072796983269 0.0 76-77 0.0 0.0 0.0 9.998294560912598 0.0 78-79 0.0 0.0 0.0 10.95049805137793 0.0 80-81 0.0 0.0 0.0 11.980456931346602 0.0 82-83 0.0 0.0 0.0 12.955968089339741 0.0 84-85 0.0 0.0 0.0 13.969251564898274 0.0 86-87 0.0 0.0 0.0 15.049678808971873 0.0 88-89 0.0 0.0 0.0 16.146655128634322 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 240 0.0 29.654554 3 CATTCCG 165 2.6739144E-9 28.75775 9 GTAAGAC 130 1.0102578E-5 25.548538 3 GACATAT 300 0.0 25.377356 1 GTACATG 4000 0.0 24.861877 1 TACATGG 4250 0.0 23.444542 2 GAGTACT 4650 0.0 23.316969 12-13 ACATGGG 4035 0.0 23.047503 3 CATGGGG 1400 0.0 23.045826 4 AGTACTT 4855 0.0 22.33242 12-13 GTGACCC 130 2.9612822E-4 21.900131 9 GTGTAGC 220 5.531183E-8 21.628428 1 GACTGTG 155 3.9380182E-5 21.427807 7 GTACTTT 5105 0.0 21.099342 14-15 ATGGGGA 735 0.0 20.657322 5 GGAGAGT 210 8.692896E-7 20.33455 6 ACCGTCT 145 6.189832E-4 19.63336 8 GTATAGG 350 6.184564E-11 19.033018 1 CTTTGCG 125 0.0060683107 18.978914 2 CATGGGA 1990 0.0 18.59743 4 >>END_MODULE