FastQCFastQC Report
Fri 27 May 2016
SRR939259_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939259_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931177
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.3075844420268054No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55440.2870788125583517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47060.2436855865619775No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36100.1869326322755501No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30450.1576758629581856No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23360.12096250110683796No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21870.11324699910986925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79700.023.22131
TACATGG82200.022.2656252
ACATGGG82350.021.7068673
GAGTACT56950.021.00709512-13
GTATCAA135700.020.4227031
GTACTTT59250.019.91510414-15
AGTACTT60200.019.28270512-13
CATGGGG50750.019.0595914
AGAGTAC106800.018.85381110-11
TAGTACG1300.007643100318.2366924
TCAACGC152900.017.9552124
CAACGCA153750.017.9166955
TATCAAC155400.017.8494262
CATGGGA34400.017.7807774
ATCAACG153950.017.771153
AACGCAG157650.017.4020256
GGTATCA110350.017.2015631
TACCTGG14550.016.9457112
ATGGGGA25250.016.8996625
ACTTTTT72000.016.52567716-17
Produced by FastQC (version 0.11.5)