##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939259_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1931177 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.694946656883342 33.0 31.0 34.0 30.0 34.0 2 31.77220265154359 33.0 31.0 34.0 30.0 34.0 3 31.816905959422673 34.0 31.0 34.0 30.0 34.0 4 35.297374088444506 37.0 35.0 37.0 33.0 37.0 5 35.32304340824275 37.0 35.0 37.0 33.0 37.0 6 35.40684359848942 37.0 35.0 37.0 33.0 37.0 7 35.3358847997879 37.0 35.0 37.0 33.0 37.0 8 35.31448800394785 37.0 35.0 37.0 33.0 37.0 9 36.90656527081671 39.0 37.0 39.0 33.0 39.0 10-11 36.78702495939005 39.0 37.0 39.0 33.0 39.0 12-13 36.669883961956884 39.0 37.0 39.0 32.0 39.0 14-15 37.85123580075778 40.0 38.0 41.0 32.5 41.0 16-17 37.75910390399223 40.0 38.0 41.0 32.0 41.0 18-19 37.661257098650196 40.0 38.0 41.0 32.0 41.0 20-21 37.6292341406303 40.0 38.0 41.0 32.0 41.0 22-23 37.63757646243715 40.0 38.0 41.0 32.0 41.0 24-25 37.53240251929264 40.0 38.0 41.0 32.0 41.0 26-27 37.42451106242463 40.0 37.0 41.0 31.5 41.0 28-29 37.26504432271096 40.0 37.0 41.0 31.0 41.0 30-31 37.071132009132256 40.0 37.0 41.0 30.5 41.0 32-33 36.8278456609622 40.0 36.0 41.0 30.0 41.0 34-35 36.51589652320838 39.5 36.0 41.0 30.0 41.0 36-37 36.25555244288846 39.0 35.5 41.0 29.5 41.0 38-39 35.6363611414179 38.5 34.5 40.5 27.0 41.0 40-41 35.60546806429447 39.0 35.0 40.0 27.0 41.0 42-43 35.77160845432604 39.0 35.0 41.0 27.0 41.0 44-45 35.66312513042564 39.0 35.0 41.0 27.0 41.0 46-47 35.37152135718269 39.0 35.0 41.0 25.5 41.0 48-49 35.098810207453795 39.0 35.0 41.0 24.0 41.0 50-51 33.97335795734932 38.0 33.5 40.0 23.0 40.5 52-53 34.07541540728789 38.0 33.5 39.5 23.0 40.5 54-55 34.52897145108915 38.0 34.0 40.0 23.0 41.0 56-57 34.40284163492005 38.0 34.0 40.0 23.0 41.0 58-59 34.15096725986277 38.0 34.0 40.0 22.0 41.0 60-61 33.800971894342155 37.5 34.0 40.0 21.5 41.0 62-63 33.393127092959375 37.0 33.0 40.0 19.5 41.0 64-65 32.97361971481641 36.5 33.0 39.5 18.0 41.0 66-67 32.57549670485926 36.0 32.0 39.0 15.5 41.0 68-69 32.149218844259224 35.5 32.0 39.0 12.5 40.0 70-71 31.70607898706333 35.0 31.0 38.0 8.0 40.0 72-73 31.249887503838334 35.0 31.0 37.0 7.0 39.5 74-75 30.77266118020254 35.0 30.5 37.0 4.0 39.0 76-77 30.29900832497487 35.0 30.0 36.0 2.0 39.0 78-79 29.888977809905565 34.0 30.0 36.0 2.0 38.0 80-81 29.452749281914606 34.0 29.0 35.5 2.0 37.0 82-83 29.013333578434292 34.0 29.0 35.0 2.0 37.0 84-85 28.57764591231151 34.0 29.0 35.0 2.0 36.0 86-87 28.141001575722992 34.0 27.5 35.0 2.0 36.0 88-89 27.686458569048824 34.0 26.5 35.0 2.0 35.5 90-91 27.24051601691611 33.5 26.0 35.0 2.0 35.0 92-93 26.75218869114535 33.0 25.0 35.0 2.0 35.0 94-95 26.226272630628888 33.0 24.5 35.0 2.0 35.0 96-97 25.560686565757564 32.5 21.5 35.0 2.0 35.0 98-99 24.691812040014973 32.0 10.0 34.5 2.0 35.0 100-101 21.880869283343785 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3231610485021008 1101 2 0.3810694642848773 1101 3 0.38733511676097265 1101 4 0.3596145509705764 1101 5 0.3975960745389031 1101 6 0.35162924906647675 1101 7 0.3201205289041269 1101 8 0.24022551962685412 1101 9 0.2780202765956048 1101 10-11 0.44054606624330006 1101 12-13 0.40281235395602266 1101 14-15 0.45009414677382864 1101 16-17 0.44091802810715564 1101 18-19 0.6076855491585889 1101 20-21 0.4215689647162648 1101 22-23 0.47380689896436934 1101 24-25 0.47860657553224684 1101 26-27 0.5388326957256737 1101 28-29 0.5484476331175188 1101 30-31 0.5829078887830619 1101 32-33 0.46815379592367634 1101 34-35 0.4242055351584284 1101 36-37 0.4538084445348147 1101 38-39 0.8395675529003483 1101 40-41 0.793418877355414 1101 42-43 0.6680951275267546 1101 44-45 0.5049699747788665 1101 46-47 0.43677032703327257 1101 48-49 0.3939580271348362 1101 50-51 0.3186785134156409 1101 52-53 0.3057776452911938 1101 54-55 0.2921752368604089 1101 56-57 0.4442825020902035 1101 58-59 0.388384027809046 1101 60-61 0.370027011181719 1101 62-63 0.4010973593819003 1101 64-65 0.20681729090063783 1101 66-67 0.12777621319293075 1101 68-69 0.2787613105906317 1101 70-71 0.22924616771684114 1101 72-73 0.3145368102941539 1101 74-75 0.13338209105926424 1101 76-77 0.10024742406826803 1101 78-79 0.2627265924890452 1101 80-81 0.21378594128227846 1101 82-83 0.178912849089361 1101 84-85 0.22814545974678424 1101 86-87 0.3661247185769021 1101 88-89 0.352775071519023 1101 90-91 0.4530180174985148 1101 92-93 0.3069720754751195 1101 94-95 0.4490340968618334 1101 96-97 0.6226799778747214 1101 98-99 0.7149061456930212 1101 100-101 0.28124170793362424 1102 1 0.3426167520713932 1102 2 0.39540899443235134 1102 3 0.3502398918347751 1102 4 0.22609163229742535 1102 5 0.232542615707942 1102 6 0.37177023177472535 1102 7 0.34097662734306056 1102 8 0.2714448609247384 1102 9 0.5626174088178999 1102 10-11 0.49036371012785906 1102 12-13 0.539462500347824 1102 14-15 0.6611543467495764 1102 16-17 0.6385374851581815 1102 18-19 0.6313990626963815 1102 20-21 0.765899434806208 1102 22-23 0.8475822352035038 1102 24-25 0.6266587914927086 1102 26-27 0.6895451807031066 1102 28-29 0.6349660493527267 1102 30-31 0.6642594414728862 1102 32-33 0.4552870063602015 1102 34-35 0.5047070658076578 1102 36-37 0.5992448255423142 1102 38-39 0.8538334540392043 1102 40-41 0.9242930766599926 1102 42-43 0.8230439272937105 1102 44-45 0.8470432906636702 1102 46-47 0.9226789539148257 1102 48-49 0.9140598693679252 1102 50-51 0.79797175834711 1102 52-53 0.8339658455287307 1102 54-55 0.8377437330127648 1102 56-57 0.8880095672154695 1102 58-59 0.9832607000216882 1102 60-61 0.994687660053799 1102 62-63 0.8590585641128925 1102 64-65 0.981302064293331 1102 66-67 0.8963411501286416 1102 68-69 0.7832252497008838 1102 70-71 1.0561316959432183 1102 72-73 0.8093397893334675 1102 74-75 0.7682885613647947 1102 76-77 0.8748016557631964 1102 78-79 0.8829577907843138 1102 80-81 0.77987351071652 1102 82-83 0.72406218925153 1102 84-85 0.8554950913450838 1102 86-87 0.8115640190788618 1102 88-89 0.9916257467563803 1102 90-91 0.8353415372667001 1102 92-93 0.8731679228631997 1102 94-95 0.9274372168068581 1102 96-97 0.8948699002931626 1102 98-99 0.915896977197125 1102 100-101 0.5996735351714158 1103 1 0.44480666386323264 1103 2 0.5682710055476221 1103 3 0.4080960228327868 1103 4 0.34023437006467105 1103 5 0.4124230170659402 1103 6 0.4277733900183449 1103 7 0.39374412149173565 1103 8 0.4866385976720764 1103 9 0.5446251457469558 1103 10-11 0.5424229028818104 1103 12-13 0.6141678226537408 1103 14-15 0.7853783824167451 1103 16-17 0.7246168993730961 1103 18-19 0.7445903603787585 1103 20-21 0.7597835369910015 1103 22-23 0.7949567852369626 1103 24-25 0.8054031593129238 1103 26-27 0.6853718330544467 1103 28-29 0.6125700967161478 1103 30-31 0.6218266213328079 1103 32-33 0.6450628324878807 1103 34-35 0.6722955267013617 1103 36-37 0.5706312407438645 1103 38-39 0.9508429121342488 1103 40-41 0.7854061783802635 1103 42-43 0.6469484915187991 1103 44-45 0.5084998577757105 1103 46-47 0.4303397421704602 1103 48-49 0.49948248920010485 1103 50-51 0.5786581004790747 1103 52-53 0.6765428213185203 1103 54-55 0.5689542309736026 1103 56-57 0.6590324260913718 1103 58-59 0.6846182457029641 1103 60-61 0.7061712923410752 1103 62-63 0.5294885484880254 1103 64-65 0.5559306059602633 1103 66-67 0.6451254566779028 1103 68-69 0.5854671163917828 1103 70-71 0.6410386058168278 1103 72-73 0.6064747837732689 1103 74-75 0.5461276214673987 1103 76-77 0.5242430126306488 1103 78-79 0.396626024202579 1103 80-81 0.4369916518060606 1103 82-83 0.3989752031859375 1103 84-85 0.3554243891733435 1103 86-87 0.3490672022761885 1103 88-89 0.37280450871976356 1103 90-91 0.3835808932307003 1103 92-93 0.3470199563344387 1103 94-95 0.43210451989970977 1103 96-97 0.6014613261992103 1103 98-99 0.7694455863798311 1103 100-101 0.6644850323042988 1104 1 0.42149550705199346 1104 2 0.47879160302328927 1104 3 0.40735260890733826 1104 4 0.36861849285827475 1104 5 0.37790494253948026 1104 6 0.3168725246744657 1104 7 0.46365839283593147 1104 8 0.5324655654056443 1104 9 0.5624485103464849 1104 10-11 0.5153142605847663 1104 12-13 0.5809565451715031 1104 14-15 0.6416042694618866 1104 16-17 0.5121854259594301 1104 18-19 0.6611887286449942 1104 20-21 0.742768107847283 1104 22-23 0.7717228968925767 1104 24-25 0.7839351150958436 1104 26-27 0.6693887483965426 1104 28-29 0.7406945911663456 1104 30-31 0.7360536502516695 1104 32-33 0.763341498774345 1104 34-35 0.7910348137667782 1104 36-37 0.6522852832223123 1104 38-39 1.2176082810054183 1104 40-41 0.9842374574110195 1104 42-43 0.902007923716738 1104 44-45 0.8790921717192219 1104 46-47 0.8313015536077017 1104 48-49 0.8061198032915442 1104 50-51 0.6891959141874793 1104 52-53 0.6528154219516509 1104 54-55 0.8068185179603375 1104 56-57 0.7315812216789794 1104 58-59 0.790609494695012 1104 60-61 0.8182725728888727 1104 62-63 0.8591488756782795 1104 64-65 0.7762415745574813 1104 66-67 0.7431426313650675 1104 68-69 0.6388522306137716 1104 70-71 0.7831752882267473 1104 72-73 0.6857545913039722 1104 74-75 0.7849323491015632 1104 76-77 0.7946862661020653 1104 78-79 0.7193236914187793 1104 80-81 0.5194556755449469 1104 82-83 0.6611120177863086 1104 84-85 0.3808469737233011 1104 86-87 0.3601815866746918 1104 88-89 0.292161880572138 1104 90-91 0.3261623965131193 1104 92-93 0.29887477722581934 1104 94-95 0.40043247434058316 1104 96-97 0.39904831538539653 1104 98-99 0.2900669822228821 1104 100-101 0.24944053133270216 1105 1 0.026435814350247 1105 2 0.08452272711545206 1105 3 0.2302276679633053 1105 4 0.16927138976165423 1105 5 0.11704412659555885 1105 6 0.20178240069373032 1105 7 0.23617677339451149 1105 8 0.13181368968612617 1105 9 0.10439288646171008 1105 10-11 0.32953538713677943 1105 12-13 0.23659547738562026 1105 14-15 0.2458425128574575 1105 16-17 0.21422214713027898 1105 18-19 0.18630009024649752 1105 20-21 0.30019603404212347 1105 22-23 0.38852350383621115 1105 24-25 0.29854660419515966 1105 26-27 0.17884761405949945 1105 28-29 0.2658002461521747 1105 30-31 0.3517368117407855 1105 32-33 0.3138016472244445 1105 34-35 0.205813716949244 1105 36-37 0.015608083344503143 1105 38-39 0.5207105174280997 1105 40-41 0.4613255456316949 1105 42-43 0.16580659551695476 1105 44-45 0.20008359707863832 1105 46-47 0.026957827926523237 1105 48-49 -0.07075035227980919 1105 50-51 -0.021736669957320487 1105 52-53 -0.04749099327552386 1105 54-55 0.03376041169774879 1105 56-57 0.14110583356153228 1105 58-59 0.09997684426336662 1105 60-61 0.2502094673306061 1105 62-63 0.20759948446904986 1105 64-65 0.14785353430241344 1105 66-67 0.10894675808918208 1105 68-69 0.07797045036593175 1105 70-71 -0.038448219906232595 1105 72-73 0.06341436227645758 1105 74-75 0.055270351948262686 1105 76-77 0.08331742620597282 1105 78-79 0.17567799296055497 1105 80-81 0.10695686567466467 1105 82-83 0.011904291268955092 1105 84-85 0.22371876499092025 1105 86-87 0.053493943690178014 1105 88-89 -0.07967248701019969 1105 90-91 -0.03394607724050758 1105 92-93 -0.07344270042602474 1105 94-95 0.1813658604150561 1105 96-97 0.28591075501165975 1105 98-99 0.19583859325155117 1105 100-101 0.03203559378860632 1106 1 0.22555411426430183 1106 2 0.23988372332525643 1106 3 0.26406739145819813 1106 4 0.18195896743596762 1106 5 0.22507093621387497 1106 6 0.2812590690298151 1106 7 0.2426071246524515 1106 8 0.23707359335451628 1106 9 0.309762229762093 1106 10-11 0.33979148824168703 1106 12-13 0.39178489451137466 1106 14-15 0.4289514021997647 1106 16-17 0.47522948638695084 1106 18-19 0.4628082858528373 1106 20-21 0.4379152997454767 1106 22-23 0.5210258379300541 1106 24-25 0.5339237381134083 1106 26-27 0.5460701351281756 1106 28-29 0.5899023156123349 1106 30-31 0.44299833536305044 1106 32-33 0.3434214527063162 1106 34-35 0.23362962341236226 1106 36-37 0.2858698728370612 1106 38-39 0.7187065973870475 1106 40-41 0.5819657365636601 1106 42-43 0.44504923616543124 1106 44-45 0.39295239936388526 1106 46-47 0.3357630810288583 1106 48-49 0.266116582730362 1106 50-51 0.20212147896714328 1106 52-53 0.22865394931503857 1106 54-55 0.28468941196361897 1106 56-57 0.2993110235969638 1106 58-59 0.41976625025966996 1106 60-61 0.4783230866909065 1106 62-63 0.2810415154707684 1106 64-65 0.46464571647921105 1106 66-67 0.2641655446076996 1106 68-69 0.38157577519141483 1106 70-71 0.31732023798424436 1106 72-73 0.3043701350624701 1106 74-75 0.36880871455824504 1106 76-77 0.5818968750506741 1106 78-79 0.7426587575540502 1106 80-81 0.7825116380464152 1106 82-83 0.4622607429516705 1106 84-85 0.553637519975986 1106 86-87 0.6646926477270796 1106 88-89 0.3587491709074797 1106 90-91 0.4646364631192199 1106 92-93 0.504622691299847 1106 94-95 0.5334337748171905 1106 96-97 0.6730379955871761 1106 98-99 0.7534829824609268 1106 100-101 0.4449491612114542 1107 1 0.20900881548512018 1107 2 0.09510384053937315 1107 3 0.21696918731351644 1107 4 0.21458226617620824 1107 5 0.11626981729281738 1107 6 0.16022056230914927 1107 7 0.19042542329533774 1107 8 0.1158257757048986 1107 9 0.22936573969543161 1107 10-11 0.22242079047368435 1107 12-13 0.3305525236838065 1107 14-15 0.2725020294835261 1107 16-17 0.30079722199967307 1107 18-19 0.2727133982317085 1107 20-21 0.2859377171082187 1107 22-23 0.16380104123749106 1107 24-25 0.25419185081030093 1107 26-27 0.3645107524818343 1107 28-29 0.2622447158407937 1107 30-31 0.3032824790055173 1107 32-33 0.4074778985093914 1107 34-35 0.5428078265186045 1107 36-37 0.48902722273354016 1107 38-39 1.0532430629205152 1107 40-41 0.7829666971223475 1107 42-43 0.34591049384827954 1107 44-45 0.41989834197699594 1107 46-47 0.4773002669813309 1107 48-49 0.3280847869399466 1107 50-51 0.28578175921662563 1107 52-53 0.34886251386393496 1107 54-55 0.44796897348155795 1107 56-57 0.24666526855241244 1107 58-59 0.1771189051010822 1107 60-61 0.21036372221729494 1107 62-63 0.2113823515462343 1107 64-65 0.4321076024193573 1107 66-67 0.33825674576036846 1107 68-69 0.4030536726063403 1107 70-71 0.2559775327134979 1107 72-73 0.30515464540470205 1107 74-75 0.3268937953944473 1107 76-77 0.24024870562889333 1107 78-79 0.4040958961276253 1107 80-81 0.2100705858452443 1107 82-83 0.19849570988378673 1107 84-85 0.27154398881948794 1107 86-87 0.33197507794054815 1107 88-89 0.4199336760575001 1107 90-91 0.26227498152235995 1107 92-93 0.11684012154576351 1107 94-95 -0.034494923171479286 1107 96-97 0.13498559321692838 1107 98-99 0.4083568442313421 1107 100-101 0.020000109763152807 1108 1 0.01573951691901243 1108 2 0.030413662408626863 1108 3 0.035141536344664104 1108 4 0.06742729434765948 1108 5 0.07976391058230803 1108 6 -0.030468756896794957 1108 7 0.07469171562462407 1108 8 0.16190965548106817 1108 9 0.15921113323042135 1108 10-11 0.2909368696742476 1108 12-13 0.2107212959982263 1108 14-15 0.1574445648500742 1108 16-17 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-1.0991893904475916 2316 88-89 -1.2730736308964872 2316 90-91 -1.217387240125504 2316 92-93 -1.4010749061716616 2316 94-95 -1.243275518012453 2316 96-97 -1.2712806448325367 2316 98-99 -1.5801873380709708 2316 100-101 -0.9468443172709584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10220.0 3 2894.0 4 2994.0 5 3118.0 6 3632.0 7 4348.0 8 5349.0 9 6074.0 10 6889.0 11 8137.0 12 9592.0 13 11720.0 14 12160.0 15 12466.0 16 14759.0 17 17673.0 18 16257.0 19 13073.0 20 13570.0 21 14340.0 22 15357.0 23 16764.0 24 18725.0 25 21407.0 26 23997.0 27 27890.0 28 32710.0 29 38167.0 30 45920.0 31 55537.0 32 69063.0 33 88158.0 34 120321.0 35 172093.0 36 261826.0 37 384908.0 38 310793.0 39 38267.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14427402857676 18.70934605211703 15.420988589759501 22.725391329546714 2 15.372931630325057 20.07598590256084 39.57891318579508 24.972169281319026 3 19.67509053361532 23.90440189714676 29.3868925066023 27.03361506263562 4 13.287286210093804 16.536026287538895 36.87301568359068 33.30367181877662 5 14.483819744271006 36.841580870142806 33.4344902025905 15.240109182995681 6 31.937435493895798 35.42738744510988 17.474382879723557 15.16079418127077 7 28.992337528276067 32.17921857201888 20.323100286143728 18.505343613561315 8 26.794668237475616 33.82498892932293 19.551282383674927 19.82906044952653 9 26.42496530925066 17.444105891308542 20.29719964241045 35.83372915703035 10-11 25.562410464627337 26.188140552193772 26.63686086066987 21.61258812250902 12-13 26.552288513521223 23.47083354502632 27.10203360060221 22.87484434085025 14-15 23.28813966215836 24.93726749957418 25.8244537366119 25.950139101655555 16-17 22.82454558343673 28.15370032599125 26.256149757431096 22.765604333140924 18-19 22.71815959926089 26.892542609382815 28.24981533924818 22.139482452108115 20-21 23.610889601695384 25.65501738924551 28.27166381535425 22.462429193704864 22-23 23.2490934845515 25.57851790494438 28.33566823842692 22.8367203720772 24-25 23.3205475102664 25.838335212685905 28.207642389166836 22.633474887880862 26-27 22.936966161644193 25.905453651406376 28.470082229326092 22.687497957623343 28-29 22.56993683459253 26.394146333366876 28.36706684910532 22.668849982935278 30-31 22.954434774310624 25.79903939457275 28.223709766673803 23.022816064442818 32-33 22.726573686095524 25.966076675127226 28.287124432465205 23.02022520631204 34-35 23.43622608448939 25.97793841400393 27.89455688460641 22.691278616900266 36-37 23.222858540151112 25.789683845516514 28.129775907121573 22.857681707210798 38-39 22.88098084973483 26.116856092422225 28.20988370248515 22.792279355357795 40-41 23.13977404214933 25.980758479919913 28.117997321788422 22.761470156142334 42-43 22.882118988964162 25.9994985601811 27.750480633187408 23.367901817667327 44-45 23.096494032049108 25.967264246367872 27.695162306780585 23.24107941480243 46-47 23.366369648483687 26.388927181559453 27.23732514049364 23.00737802946322 48-49 23.40500029004606 26.310923654934154 27.193886297261578 23.09018975775821 50-51 22.90905827636795 26.464296490516176 27.468708056309893 23.157937176805984 52-53 23.318346338999152 26.640527003903518 26.968941960167754 23.072184696929572 54-55 23.033754622397765 26.222110211383182 27.261782335197115 23.482352831021938 56-57 23.231124931981093 26.462884230454947 27.174726630939354 23.131264206624603 58-59 23.637729610007437 26.100100344707467 27.047759921387982 23.214410123897117 60-61 23.2167059547418 26.253060936012407 27.668263134481624 22.86196997476417 62-63 23.049836147790064 26.37659007397411 27.557267634987532 23.016306143248293 64-65 22.796250433423655 26.594329244866096 27.296052872873755 23.3133674488365 66-67 22.956391396799944 26.485812649028706 27.031720124930477 23.52607582924087 68-69 22.77147584828885 26.246116941841287 27.36984925669031 23.612557953179554 70-71 23.090084830775506 26.290178069704425 27.15659249176875 23.463144607751317 72-73 23.09228099054881 26.11670341493565 27.368936665685094 23.422078928830448 74-75 22.84851191955003 26.844319388841615 26.884980381006248 23.422188310602113 76-77 22.98882980881568 26.407301561870916 26.948515374748105 23.6553532545653 78-79 23.123252133327025 26.424025368479032 27.046899127841513 23.405823370352437 80-81 23.185770335744436 26.55980532273197 26.89973122138246 23.35469312014113 82-83 23.1809691545632 26.490737942524422 27.089457020235407 23.238835882676963 84-85 23.086814144538785 26.47826596940775 26.928725674980843 23.50619421107262 86-87 22.992139746168576 26.51747217232947 27.01168348628864 23.478704595213312 88-89 22.955311748226176 26.55173140282735 26.69307040914183 23.799886439804645 90-91 23.113984313011354 26.492540225549238 26.77722134783231 23.616254113607095 92-93 23.344420524094154 26.29240785425786 26.91949971057099 23.443671911077 94-95 23.109079954296373 26.195896038810623 26.98748873538722 23.707535271505783 96-97 22.87977826767196 26.237969944176694 27.178833865690024 23.703417922461327 98-99 22.98336326724287 26.275616385329542 26.718641715036117 24.022378632391472 100-101 23.166928584173316 25.574202764205054 27.21257869879799 24.04628995282364 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2408.0 1 1389.0 2 427.5 3 465.5 4 376.5 5 366.5 6 588.0 7 1138.5 8 1849.5 9 2250.0 10 2101.0 11 1871.5 12 1880.5 13 2017.0 14 2226.5 15 2339.0 16 2385.5 17 2466.0 18 2559.0 19 2727.0 20 2948.0 21 3263.5 22 3801.0 23 4545.0 24 5437.0 25 6622.0 26 8298.0 27 10226.0 28 12215.5 29 14661.5 30 17540.0 31 20692.5 32 24090.5 33 27367.5 34 30456.0 35 33596.0 36 37926.5 37 41888.5 38 44803.0 39 47118.0 40 49625.5 41 53801.0 42 56930.0 43 60301.5 44 64766.5 45 68297.0 46 71140.0 47 77111.5 48 87355.0 49 86855.5 50 78046.5 51 73106.0 52 69694.5 53 68923.5 54 68516.5 55 65177.5 56 60342.5 57 55716.0 58 50933.0 59 44965.5 60 38261.5 61 31688.0 62 26129.5 63 21005.0 64 16717.0 65 13387.5 66 10283.0 67 8346.0 68 6343.0 69 4326.5 70 3185.5 71 2324.5 72 1739.5 73 1437.0 74 1156.5 75 922.0 76 698.5 77 463.0 78 317.0 79 223.0 80 140.5 81 91.5 82 73.0 83 50.5 84 39.0 85 24.0 86 12.5 87 11.0 88 9.0 89 8.5 90 9.0 91 5.5 92 4.0 93 5.5 94 3.5 95 1.5 96 1.0 97 1.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29132492775131436 2 0.17869931135261033 3 0.1781297105340422 4 0.20070661570638007 5 0.21422168967422459 6 0.10853484688353268 7 0.12562287144057743 8 0.13768805241570298 9 0.14027714704555821 10-11 0.12790127471485005 12-13 0.11821806079919137 14-15 0.13212149896151415 16-17 0.11068379542631256 18-19 0.13673008740265652 20-21 0.16143005017147574 22-23 0.16518423738476587 24-25 0.18908158081832996 26-27 0.16963748014811691 28-29 0.16689303984047035 30-31 0.15627775185806375 32-33 0.16629754807560362 34-35 0.3493206474600723 36-37 0.4709304222243741 38-39 0.43683204594918024 40-41 0.4273041777113128 42-43 0.4511756301985784 44-45 0.4548262536266743 46-47 0.4581661856991876 48-49 0.46958409301684934 50-51 0.4638104119922721 52-53 0.46945463828535655 54-55 0.4527808688690887 56-57 0.4141774679379467 58-59 0.404261235505601 60-61 0.35879673380534255 62-63 0.4669691074406955 64-65 0.4650790683609012 66-67 0.47370075347831925 68-69 0.4850409879570853 70-71 0.4781022143490732 72-73 0.47074918560028417 74-75 0.47574613823590484 76-77 0.45252195940610307 78-79 0.43890332165306445 80-81 0.43522680727866997 82-83 0.44822406232054335 84-85 0.45032122897072613 86-87 0.45153810344675815 88-89 0.4581920766454861 90-91 0.4764969756785629 92-93 0.4810278912808096 94-95 0.47789508677868475 96-97 0.4774290497453108 98-99 0.47458104565246995 100-101 0.45650916513608025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1931177.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.474687696831005 #Duplication Level Percentage of deduplicated Percentage of total 1 79.61665878696932 46.55559258033187 2 11.803028820494276 13.80356848310197 3 3.623556440418893 6.35658993616006 4 1.5889004539999116 3.716418313159914 5 0.8667405574285232 2.5341191704905057 6 0.5160124267338966 1.8104199300569095 7 0.3539814651886945 1.4489268939162898 8 0.2499301292765921 1.1691669004381857 9 0.1910457408865752 1.0054206030736965 >10 1.0640192758272538 11.324179791547145 >50 0.08261840145993722 3.3189334453490886 >100 0.04066702806845342 4.374912577558474 >500 0.001242707045908777 0.4434757488224144 >1k 0.0014202366238957435 1.5421993737139004 >5k 1.7752957798696794E-4 0.5960762522795648 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5940 0.3075844420268054 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5544 0.2870788125583517 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4706 0.2436855865619775 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3610 0.1869326322755501 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 0.1576758629581856 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2336 0.12096250110683796 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2187 0.11324699910986925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.178189259710529E-5 0.0 0.0 0.0 6 0.0 5.178189259710529E-5 0.0 0.0 0.0 7 0.0 5.178189259710529E-5 0.0 0.0 0.0 8 0.0 5.178189259710529E-5 0.0 0.0 0.0 9 0.0 5.178189259710529E-5 0.0 0.0 0.0 10-11 0.0 5.178189259710529E-5 0.0 0.0 0.0 12-13 0.0 5.178189259710529E-5 0.0 0.0 0.0 14-15 0.0 5.178189259710529E-5 0.0 0.0 2.5890946298552645E-5 16-17 2.5890946298552645E-5 5.178189259710529E-5 0.0 0.0 5.178189259710529E-5 18-19 5.178189259710529E-5 5.178189259710529E-5 0.0 0.0 1.0356378519421058E-4 20-21 5.178189259710529E-5 5.178189259710529E-5 0.0 0.0 1.0356378519421058E-4 22-23 5.178189259710529E-5 5.178189259710529E-5 0.0 7.767283889565793E-5 1.0356378519421058E-4 24-25 1.0356378519421058E-4 5.178189259710529E-5 0.0 3.6247324817973705E-4 1.0356378519421058E-4 26-27 1.0356378519421058E-4 5.178189259710529E-5 0.0 5.696008185681581E-4 1.0356378519421058E-4 28-29 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.0011909835297334217 1.0356378519421058E-4 30-31 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.0033140411262147385 1.0356378519421058E-4 32-33 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.008336884708133952 1.0356378519421058E-4 34-35 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.01701035171814909 1.0356378519421058E-4 36-37 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.03093968082677041 1.0356378519421058E-4 38-39 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.051963129221195156 1.0356378519421058E-4 40-41 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.0744105796620403 1.0356378519421058E-4 42-43 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.09999083460501032 1.0356378519421058E-4 44-45 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.13300179113566493 1.0356378519421058E-4 46-47 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.17378003155588534 1.0356378519421058E-4 48-49 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.2205390805710714 1.0356378519421058E-4 50-51 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.2708969711217563 1.0356378519421058E-4 52-53 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.32213515384659197 1.0356378519421058E-4 54-55 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.3841957521242227 1.0356378519421058E-4 56-57 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.4617391362883878 1.0356378519421058E-4 58-59 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.5473346047514029 1.0356378519421058E-4 60-61 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.6418106677948215 1.0356378519421058E-4 62-63 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.7381508789717359 1.0356378519421058E-4 64-65 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.8479026003313006 1.294547314927632E-4 66-67 2.0712757038842116E-4 1.0356378519421058E-4 0.0 0.9711952866050082 1.5534567779131587E-4 68-69 2.0712757038842116E-4 1.0356378519421058E-4 0.0 1.1163917134472916 1.5534567779131587E-4 70-71 2.0712757038842116E-4 1.0356378519421058E-4 0.0 1.2764754344112426 1.8123662408986853E-4 72-73 2.0712757038842116E-4 1.0356378519421058E-4 0.0 1.442255163560875 2.589094629855264E-4 74-75 2.0712757038842116E-4 1.0356378519421058E-4 0.0 1.6187796354244068 3.3658230188118437E-4 76-77 2.0712757038842116E-4 1.0356378519421058E-4 0.0 1.8178551215139782 3.62473248179737E-4 78-79 2.0712757038842116E-4 1.0356378519421058E-4 0.0 2.0476631608599316 3.62473248179737E-4 80-81 2.0712757038842116E-4 1.0356378519421058E-4 0.0 2.305019167067545 3.62473248179737E-4 82-83 2.0712757038842116E-4 1.0356378519421058E-4 0.0 2.563825066267877 3.62473248179737E-4 84-85 2.0712757038842116E-4 1.5534567779131587E-4 0.0 2.8401073542197324 3.62473248179737E-4 86-87 2.0712757038842116E-4 1.5534567779131587E-4 0.0 3.133503557674931 3.62473248179737E-4 88-89 2.0712757038842116E-4 1.5534567779131587E-4 0.0 3.4548360921862677 3.62473248179737E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7970 0.0 23.2213 1 TACATGG 8220 0.0 22.265625 2 ACATGGG 8235 0.0 21.706867 3 GAGTACT 5695 0.0 21.007095 12-13 GTATCAA 13570 0.0 20.422703 1 GTACTTT 5925 0.0 19.915104 14-15 AGTACTT 6020 0.0 19.282705 12-13 CATGGGG 5075 0.0 19.059591 4 AGAGTAC 10680 0.0 18.853811 10-11 TAGTACG 130 0.0076431003 18.236692 4 TCAACGC 15290 0.0 17.955212 4 CAACGCA 15375 0.0 17.916695 5 TATCAAC 15540 0.0 17.849426 2 CATGGGA 3440 0.0 17.780777 4 ATCAACG 15395 0.0 17.77115 3 AACGCAG 15765 0.0 17.402025 6 GGTATCA 11035 0.0 17.201563 1 TACCTGG 1455 0.0 16.945711 2 ATGGGGA 2525 0.0 16.899662 5 ACTTTTT 7200 0.0 16.525677 16-17 >>END_MODULE