FastQCFastQC Report
Fri 27 May 2016
SRR939259_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939259_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931177
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61560.31876933082778014No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT60860.3151445983459828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54460.2820041870838354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32990.17082846367785035No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32730.16948213447032562No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21200.1097776123058632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT63450.021.87729512-13
GTACATG78200.020.93691
GTACTTT67250.020.4999714-15
TACATGG79250.020.4183562
GTATCAA149250.019.9627651
AGTACTT66250.019.26929312-13
ACATGGG80750.019.1575813
CGTAAGA2001.296443E-518.9812852
ATTCGCG4400.018.89202580-81
AGAGTAC114200.018.53371610-11
TATAGCG1851.53354E-417.955273
GTGTAAG7500.017.7687721
GGTATCA121450.017.7133871
CGCGGAA4450.017.68797982-83
ATCAACG168850.017.6210633
CAACGCA169450.017.614685
TCAACGC169250.017.5794184
TATCAAC170450.017.4556562
ACTTTTT79800.017.36428316-17
AACGCAG172750.017.3605986
Produced by FastQC (version 0.11.5)