##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939259_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1931177 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.290095625621056 33.0 31.0 34.0 31.0 34.0 2 32.50485636479722 34.0 31.0 34.0 31.0 34.0 3 32.592240379830535 34.0 31.0 34.0 31.0 34.0 4 36.1181823312933 37.0 35.0 37.0 35.0 37.0 5 36.06047814363986 37.0 35.0 37.0 35.0 37.0 6 36.0912516045914 37.0 35.0 37.0 35.0 37.0 7 36.03610906716474 37.0 35.0 37.0 35.0 37.0 8 36.00717541685718 37.0 35.0 37.0 35.0 37.0 9 37.70830327825984 39.0 37.0 39.0 35.0 39.0 10-11 37.62440806824025 39.0 37.0 39.0 35.0 39.0 12-13 37.543745860684965 39.0 37.0 39.0 35.0 39.0 14-15 38.82579924056677 40.0 38.0 41.0 35.0 41.0 16-17 38.82491998403046 40.0 38.0 41.0 34.5 41.0 18-19 38.93139598286434 40.0 38.0 41.0 35.0 41.0 20-21 38.910002293937836 40.0 38.0 41.0 35.0 41.0 22-23 38.83084253799626 40.0 38.0 41.0 35.0 41.0 24-25 38.75049827126151 40.0 38.0 41.0 34.0 41.0 26-27 38.58644598604892 40.0 38.0 41.0 34.0 41.0 28-29 38.41564652023092 40.0 38.0 41.0 34.0 41.0 30-31 38.2268432670853 40.0 38.0 41.0 33.5 41.0 32-33 38.178205312097234 40.0 38.0 41.0 33.5 41.0 34-35 38.0759769819131 40.0 38.0 41.0 33.0 41.0 36-37 37.91453864663881 40.0 37.0 41.0 33.0 41.0 38-39 37.779243953298945 40.0 37.0 41.0 33.0 41.0 40-41 37.59422569759271 40.0 37.0 41.0 33.0 41.0 42-43 37.39701254727039 40.0 37.0 41.0 32.0 41.0 44-45 37.14024685463839 40.0 36.0 41.0 31.0 41.0 46-47 36.823098038139435 40.0 36.0 41.0 31.0 41.0 48-49 36.504880184467815 39.0 36.0 41.0 30.0 41.0 50-51 36.2335583429173 39.0 35.0 41.0 30.0 41.0 52-53 35.949014512911035 39.0 35.0 40.0 29.0 41.0 54-55 35.674393388073696 38.5 35.0 40.0 28.0 41.0 56-57 35.323157846225385 38.0 34.5 40.0 28.0 41.0 58-59 34.96903753514049 38.0 34.0 40.0 27.0 41.0 60-61 34.588850219322204 37.5 34.0 40.0 26.0 41.0 62-63 34.21515039791795 37.0 33.5 40.0 26.0 41.0 64-65 33.86777260706812 36.0 33.0 39.0 26.0 41.0 66-67 33.414436118491466 36.0 33.0 39.0 25.0 40.0 68-69 32.91697006540571 35.0 32.0 38.5 23.0 40.0 70-71 32.39912835540191 35.0 31.5 38.0 22.0 40.0 72-73 31.881779867925104 35.0 31.0 37.0 20.5 39.0 74-75 31.346425262935504 35.0 31.0 36.5 19.5 39.0 76-77 29.43224624154078 32.5 28.5 35.0 15.5 37.0 78-79 30.3940697823141 34.0 30.0 35.0 17.5 37.0 80-81 30.38851436196682 34.0 30.0 35.0 16.5 37.0 82-83 30.009855647618007 34.0 30.0 35.0 10.0 36.5 84-85 29.53222050594016 34.0 29.5 35.0 7.0 36.0 86-87 28.907767646362814 34.0 29.0 35.0 2.0 36.0 88-89 28.286754916820158 33.0 28.0 35.0 2.0 35.0 90-91 27.7565083366258 33.0 27.0 35.0 2.0 35.0 92-93 27.33805471999718 33.0 26.0 35.0 2.0 35.0 94-95 26.753503174488927 32.5 25.0 34.5 2.0 35.0 96-97 25.93220895857811 32.0 24.5 34.0 2.0 35.0 98-99 24.832940740284293 32.0 11.0 34.0 2.0 35.0 100-101 20.44557800760883 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1815151433151101 1101 2 0.2185687725569494 1101 3 0.27345972420587117 1101 4 0.13648540080956195 1101 5 0.10513700812059312 1101 6 0.1358147435867636 1101 7 0.20120105694783774 1101 8 0.12348407620820012 1101 9 0.2795629829349764 1101 10-11 0.22325920126674959 1101 12-13 0.22658165883859027 1101 14-15 0.2459720997738728 1101 16-17 0.2750515681581902 1101 18-19 0.25075776516317205 1101 20-21 0.2685514521439458 1101 22-23 0.31392115096998197 1101 24-25 0.254725137200829 1101 26-27 0.18020136284596333 1101 28-29 0.22791011964248753 1101 30-31 0.30826860259866606 1101 32-33 0.3017550095203916 1101 34-35 0.30203176486668326 1101 36-37 0.296081971708567 1101 38-39 0.3454068109128414 1101 40-41 0.38724616269229983 1101 42-43 0.3305047954880749 1101 44-45 0.4300018020639911 1101 46-47 0.4904136273327495 1101 48-49 0.41618096935972915 1101 50-51 0.4505803175393055 1101 52-53 0.3225697730134769 1101 54-55 0.27427213899387226 1101 56-57 0.2394444459014906 1101 58-59 0.2676011827231193 1101 60-61 0.34123101514763476 1101 62-63 0.2862170451653512 1101 64-65 0.3265003812950127 1101 66-67 0.19954268560258015 1101 68-69 0.2736454115999365 1101 70-71 0.34731096959274765 1101 72-73 0.342405643810725 1101 74-75 0.31988271551124114 1101 76-77 0.37128165060139295 1101 78-79 0.42398621336388587 1101 80-81 0.5899607684711832 1101 82-83 0.5735099961276262 1101 84-85 0.42300548823767414 1101 86-87 0.45418443761146676 1101 88-89 0.6946799244633297 1101 90-91 1.032483275534286 1101 92-93 0.8602157913544062 1101 94-95 0.6630423236564447 1101 96-97 0.5478472132064738 1101 98-99 0.6573644812612223 1101 100-101 0.15648225007176464 1102 1 0.21015666725271842 1102 2 0.2976100747413071 1102 3 0.2832464471150473 1102 4 0.12165212319258956 1102 5 0.18478145861077877 1102 6 0.1532636957449114 1102 7 0.20678563277737538 1102 8 0.18140492133447594 1102 9 0.23196174866254893 1102 10-11 0.24345552604029308 1102 12-13 0.2575496348717863 1102 14-15 0.35962465041747294 1102 16-17 0.3984443222937699 1102 18-19 0.4082019306337017 1102 20-21 0.4345243913177157 1102 22-23 0.4011723282320574 1102 24-25 0.31218896915600425 1102 26-27 0.4029765957799185 1102 28-29 0.37299952996234964 1102 30-31 0.3530347190101466 1102 32-33 0.4791349910281255 1102 34-35 0.5133614566386484 1102 36-37 0.6344359799819657 1102 38-39 0.734233103559113 1102 40-41 0.640735600218008 1102 42-43 0.6442428135299068 1102 44-45 0.8052596640059093 1102 46-47 0.9600362874862753 1102 48-49 0.8674915015482725 1102 50-51 1.0057502609909506 1102 52-53 0.9098485411023063 1102 54-55 0.7637277912313891 1102 56-57 0.9231911710949348 1102 58-59 0.9891953036954177 1102 60-61 1.0272640894907923 1102 62-63 1.0270110129123182 1102 64-65 0.8971798984427082 1102 66-67 1.0323823784075898 1102 68-69 0.9799729827281283 1102 70-71 0.7042671303356158 1102 72-73 0.7798711953802275 1102 74-75 0.9262305947712157 1102 76-77 0.8686282792911051 1102 78-79 0.7740345614754212 1102 80-81 1.0674992689033296 1102 82-83 0.9416635410034644 1102 84-85 0.7390325824295516 1102 86-87 0.707329952273426 1102 88-89 0.9309838059793378 1102 90-91 0.9192609492939354 1102 92-93 0.9824603179439393 1102 94-95 1.0262757183098579 1102 96-97 0.8407557611654255 1102 98-99 0.3887132427860074 1102 100-101 0.7631674753123647 1103 1 0.18970230502469576 1103 2 0.21588284308003836 1103 3 0.2472702388757213 1103 4 0.16730205210651405 1103 5 0.23030469947036636 1103 6 0.18077163934185592 1103 7 0.23879164214205417 1103 8 0.21494142476976918 1103 9 0.22708809760239745 1103 10-11 0.1883961298176473 1103 12-13 0.1806541448385559 1103 14-15 0.3189485073137206 1103 16-17 0.22084878478450776 1103 18-19 0.19071725157037633 1103 20-21 0.38606461093593936 1103 22-23 0.32864309720294926 1103 24-25 0.3493252048828097 1103 26-27 0.2027774779894571 1103 28-29 0.31820342734759066 1103 30-31 0.4605967161769371 1103 32-33 0.451399264868833 1103 34-35 0.40826347814490305 1103 36-37 0.33401893314449893 1103 38-39 0.31113723141422867 1103 40-41 0.3240522550903151 1103 42-43 0.3520435345001047 1103 44-45 0.37447581046242107 1103 46-47 0.1830472530412024 1103 48-49 0.2838290778650361 1103 50-51 0.3681558368009519 1103 52-53 0.2215932915460357 1103 54-55 0.23653624785097094 1103 56-57 0.33355713321392244 1103 58-59 0.24595967799078977 1103 60-61 0.26859843462232647 1103 62-63 0.27173042446549545 1103 64-65 0.19425794724192968 1103 66-67 0.1520859539539856 1103 68-69 0.2434275100345218 1103 70-71 0.19178803706378034 1103 72-73 0.08716554803625698 1103 74-75 0.16909959292464194 1103 76-77 0.31059363330743395 1103 78-79 0.24916531250524443 1103 80-81 0.3600031111344748 1103 82-83 0.524755548522851 1103 84-85 0.5831602072076869 1103 86-87 0.44555084249374843 1103 88-89 0.5515540429883714 1103 90-91 0.740245289882612 1103 92-93 0.5377826878551453 1103 94-95 0.4332479761853101 1103 96-97 0.07711728024477438 1103 98-99 -0.3074593703278694 1103 100-101 -0.46397972724903624 1104 1 0.1609153160902963 1104 2 0.18084387522309697 1104 3 0.19438398624213704 1104 4 0.048904414412845654 1104 5 0.1295976064454223 1104 6 0.08361795157444618 1104 7 0.10919495521247313 1104 8 0.14340561720884892 1104 9 0.2307645658763775 1104 10-11 0.16176998267204823 1104 12-13 0.18638523043060928 1104 14-15 0.15726447648690822 1104 16-17 0.17572982281947702 1104 18-19 0.2063958260788965 1104 20-21 0.15350997378929065 1104 22-23 0.18361049966686238 1104 24-25 0.2146415460776936 1104 26-27 0.3045007064328189 1104 28-29 0.1731325890020301 1104 30-31 0.21464064243480863 1104 32-33 0.19782603104343366 1104 34-35 0.348375852619057 1104 36-37 0.2641002554518863 1104 38-39 0.17455553802935952 1104 40-41 0.3707936974895816 1104 42-43 0.38205297038049224 1104 44-45 0.36260164570149556 1104 46-47 0.398688908644786 1104 48-49 0.31925973862215073 1104 50-51 0.31413557892885535 1104 52-53 0.4193413957146319 1104 54-55 0.4396818140887717 1104 56-57 0.3238140673899963 1104 58-59 0.21867604071384505 1104 60-61 0.3399239964160117 1104 62-63 0.5157665892553709 1104 64-65 0.5585877385006128 1104 66-67 0.552960740010036 1104 68-69 0.45685654925542707 1104 70-71 0.4329336372934094 1104 72-73 0.47880871942370007 1104 74-75 0.23453780112712153 1104 76-77 0.5165600493787714 1104 78-79 0.6006014988232806 1104 80-81 0.7885886355245582 1104 82-83 0.6998875973426699 1104 84-85 0.4660002813098778 1104 86-87 0.42068896577960047 1104 88-89 0.6203731793818541 1104 90-91 0.7559378702843098 1104 92-93 0.7996999718826991 1104 94-95 0.7442914813221684 1104 96-97 0.45386085657563413 1104 98-99 0.5884482021077773 1104 100-101 0.6067457843122774 1105 1 0.10267299576835143 1105 2 0.13477797102035538 1105 3 -0.0037229791628519138 1105 4 0.07410633099474495 1105 5 0.03566671235991237 1105 6 0.09362194920890232 1105 7 0.10671077530270878 1105 8 0.05162611723453381 1105 9 0.05114553353217843 1105 10-11 0.11554128974834299 1105 12-13 0.09960082126507075 1105 14-15 0.14795308178702982 1105 16-17 0.091456474727039 1105 18-19 0.08119688294411986 1105 20-21 0.1197780183333279 1105 22-23 0.09934405958286874 1105 24-25 0.12523044178448828 1105 26-27 0.09139044770987681 1105 28-29 0.22104938894820947 1105 30-31 0.28306221356221783 1105 32-33 0.16831787223492967 1105 34-35 0.26394196841710027 1105 36-37 0.1023773200528737 1105 38-39 0.24062132809978465 1105 40-41 0.3243586653568329 1105 42-43 0.2964945825414773 1105 44-45 0.11636223034728488 1105 46-47 0.09486598673141344 1105 48-49 0.23341571965096364 1105 50-51 0.1628027387672546 1105 52-53 0.009562238737949258 1105 54-55 0.11317344293409093 1105 56-57 0.12847420989320568 1105 58-59 0.1282485581332864 1105 60-61 0.0895884323966456 1105 62-63 0.24313647916874714 1105 64-65 0.24755180454237546 1105 66-67 0.24478832573015552 1105 68-69 0.1486222632476597 1105 70-71 0.20091060298974384 1105 72-73 0.1612185871833951 1105 74-75 0.25409106923083513 1105 76-77 0.36354105445796137 1105 78-79 0.47165646284306817 1105 80-81 0.5875358550361334 1105 82-83 0.5722582366247906 1105 84-85 0.42895518492964513 1105 86-87 0.333050516525379 1105 88-89 0.47343428311941693 1105 90-91 0.5966342395852386 1105 92-93 0.4936564255994895 1105 94-95 0.6432504444481211 1105 96-97 0.40161363637325564 1105 98-99 0.48105720372334915 1105 100-101 0.18924617701754087 1106 1 0.1319836497474398 1106 2 0.1479416420089592 1106 3 0.2131962642815921 1106 4 0.04734926842866116 1106 5 0.0771208373684118 1106 6 0.12424340555980251 1106 7 0.10135008824440916 1106 8 0.07025249527735866 1106 9 0.1543681287972163 1106 10-11 0.15190832677850352 1106 12-13 0.11357910646351144 1106 14-15 0.16804329219554148 1106 16-17 0.049860140027249145 1106 18-19 0.08861342749594314 1106 20-21 0.1941428665708571 1106 22-23 0.27232514569392663 1106 24-25 0.26872465755986497 1106 26-27 0.24992488409577618 1106 28-29 0.20373535903471662 1106 30-31 0.22189040570605556 1106 32-33 0.21141977475327423 1106 34-35 0.2723539808078925 1106 36-37 0.1663562235543239 1106 38-39 0.2207815304085372 1106 40-41 0.2129651958731742 1106 42-43 0.34073570834758726 1106 44-45 0.3555032838119345 1106 46-47 0.3366672888775426 1106 48-49 0.16018545742866053 1106 50-51 0.41457726839324494 1106 52-53 0.3341496760574785 1106 54-55 0.20590189043707596 1106 56-57 0.26907940487505755 1106 58-59 0.18172403932759806 1106 60-61 0.18649969973231606 1106 62-63 0.24662525387768852 1106 64-65 0.1350979454041621 1106 66-67 0.2536393332549096 1106 68-69 0.3600664335777779 1106 70-71 0.12315590852417557 1106 72-73 -0.004554631640221629 1106 74-75 0.03268912899600451 1106 76-77 0.15829537175869746 1106 78-79 0.317750024453872 1106 80-81 0.12606231531325918 1106 82-83 0.41221359514778655 1106 84-85 0.36282767848383557 1106 86-87 0.33758886058626913 1106 88-89 0.39308426178796196 1106 90-91 0.494617335181065 1106 92-93 0.5397385486046922 1106 94-95 0.8218719997275024 1106 96-97 0.7473393076957393 1106 98-99 0.5464303805023611 1106 100-101 0.41077332361100005 1107 1 0.04035738495775831 1107 2 0.05255751589020008 1107 3 0.07449451126199591 1107 4 0.06226226647509492 1107 5 0.0756069839055371 1107 6 0.08631431815545199 1107 7 0.09672577702339424 1107 8 0.0693422361212157 1107 9 0.1310388843285395 1107 10-11 0.1690588424297701 1107 12-13 0.05520569576626144 1107 14-15 0.19183172265564252 1107 16-17 0.14675081806485224 1107 18-19 0.10428416355648551 1107 20-21 0.15926988098572537 1107 22-23 0.22707098705895845 1107 24-25 0.12203826049866251 1107 26-27 0.1497825851153891 1107 28-29 0.13255747395128026 1107 30-31 0.1373060744950365 1107 32-33 0.19512272847122603 1107 34-35 0.23207055109418206 1107 36-37 0.22071216246798997 1107 38-39 0.35245027395883 1107 40-41 0.40383242459648017 1107 42-43 0.25542041145057937 1107 44-45 0.2651977872095941 1107 46-47 0.21653417361699212 1107 48-49 0.08480026041677036 1107 50-51 0.1978341788990292 1107 52-53 0.10273940716594865 1107 54-55 0.0865613983454594 1107 56-57 0.13191990021180544 1107 58-59 0.14861559355049536 1107 60-61 0.2042659789303798 1107 62-63 0.1570783065721173 1107 64-65 0.034679810315864756 1107 66-67 -0.038002183648984555 1107 68-69 0.14136085130397902 1107 70-71 0.06544531576860635 1107 72-73 0.2511803255565006 1107 74-75 0.027310324891562487 1107 76-77 0.16572590973646228 1107 78-79 0.16119518499048624 1107 80-81 0.17422411103877167 1107 82-83 0.30281293833333933 1107 84-85 0.3400509175136719 1107 86-87 0.5317198794923996 1107 88-89 0.7350975084114033 1107 90-91 0.7539511423034817 1107 92-93 0.6601742723012833 1107 94-95 0.8451030789930662 1107 96-97 0.8633535408389115 1107 98-99 1.032271875339358 1107 100-101 0.8165209368173869 1108 1 -0.028850160760761412 1108 2 -0.05142686962602028 1108 3 -9.226121090932793E-4 1108 4 0.01935608798538624 1108 5 0.04156888353953292 1108 6 -0.0076994887403074586 1108 7 0.0018081761157517917 1108 8 -6.168688022114566E-4 1108 9 -0.08837153430115308 1108 10-11 -0.06669518458149071 1108 12-13 0.06772260184219192 1108 14-15 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 18.0 5 53.0 6 87.0 7 222.0 8 567.0 9 1079.0 10 1646.0 11 2808.0 12 3869.0 13 5252.0 14 6885.0 15 9085.0 16 11917.0 17 16141.0 18 15945.0 19 12308.0 20 11760.0 21 13021.0 22 13451.0 23 15029.0 24 17073.0 25 20053.0 26 23678.0 27 28707.0 28 34466.0 29 41574.0 30 50522.0 31 61285.0 32 75885.0 33 97254.0 34 132324.0 35 190314.0 36 293696.0 37 430840.0 38 278089.0 39 14269.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.25908089779923 19.476950257925903 15.852069509382499 22.411899334892365 2 15.930543911821651 20.677545351876084 38.659843194072835 24.732067542229426 3 19.766494733522613 24.094736008144256 29.661600153688656 26.47716910464447 4 13.793194512983534 17.27345551443498 36.34141251682264 32.591937455758845 5 14.373928438460068 36.99360545408318 33.4515168728708 15.180949234585956 6 31.987746332935824 35.906341055221766 17.04747933514121 15.058433276701203 7 28.878709719513022 32.37372856035464 20.15025033955976 18.597311380572574 8 26.785685620738025 34.304053952589534 19.13724117468259 19.773019251989847 9 26.20945671991744 17.378003155588534 20.180801656192052 36.23173846830197 10-11 25.57606060966965 26.415030833527947 26.427587942482745 21.58132061431966 12-13 26.52423884501524 23.546547002165 27.021836941927123 22.907377210892633 14-15 23.131221203973283 25.103079904564513 25.764759035754647 26.00093985570755 16-17 22.697336653271442 28.262410089684415 26.29600180823914 22.744251448805006 18-19 22.638154844031526 26.959849066022223 28.314567992396338 22.087428097549914 20-21 23.500001165092886 25.71706936160418 28.372083028143724 22.41084644515921 22-23 23.199996685958872 25.606378907785256 28.414640398057767 22.77898400819811 24-25 23.27303504546709 25.866919500387585 28.332281297882066 22.527764156263252 26-27 22.905032995439047 26.00291221514766 28.514179960811443 22.577874828601846 28-29 22.518391633703178 26.451511176862606 28.447521899856927 22.58257528957729 30-31 22.830610555117424 25.825442204417303 28.302636164370227 23.041311076095045 32-33 22.65623503179667 25.99171386154661 28.373577357228257 22.978473749428456 34-35 23.380311373971114 26.002323485404165 28.001002511197342 22.616362629427382 36-37 23.159463294390587 25.801278067776952 28.284111152539566 22.7551474852929 38-39 22.849297689170818 26.146002977539002 28.31753511554146 22.68716421774872 40-41 23.093173108565725 26.019945415110037 28.198240348025926 22.68864112829831 42-43 22.881292521145845 26.091571877484725 27.78191890259358 23.24521669877585 44-45 23.126542632118436 26.002204379709454 27.741629299142406 23.129623689029703 46-47 23.33807138141287 26.412779784328194 27.34123008013256 22.907918754126374 48-49 23.400451539740157 26.354386201111247 27.242267640860202 23.002894618288394 50-51 22.91555065813542 26.538836061248706 27.470496596503015 23.075116684112864 52-53 23.363846692378115 26.620825859476298 26.97993347058994 23.035393977555646 54-55 23.06948042566787 26.285653774874078 27.178037020946295 23.466828778511758 56-57 23.28196099499654 26.591340707993243 27.038118231894003 23.08858006511622 58-59 23.637756593438034 26.266846560082147 26.942948569073195 23.152448277406627 60-61 23.204297300180304 26.383490595836918 27.601925468861815 22.810286635120956 62-63 23.075080819342165 26.47213409149635 27.402326789249358 23.050458299912123 64-65 22.8328630674454 26.642534578653326 27.210556049497274 23.314046304404 66-67 22.96419940460933 26.48237932568305 27.025340609755023 23.5280806599526 68-69 22.75498698316161 26.333316504004735 27.30076987705528 23.610926635778377 70-71 23.079577894724306 26.359002825737882 27.11475954819263 23.446659731345186 72-73 23.09793457564998 26.197883466921983 27.227695855946916 23.476486101481118 74-75 22.86909634336798 26.874686719387565 26.782540793796112 23.473676143448344 76-77 22.97830863696007 26.399996885103132 26.963144280724887 23.65855019721191 78-79 23.050978316816607 26.486272081127854 26.99697179021673 23.46577781183881 80-81 23.120392051674266 26.541083299588745 26.910288767350842 23.428235881386144 82-83 23.113365579643915 26.468858110882636 27.109451904201425 23.308324405272018 84-85 23.025647053584418 26.527734122765544 26.843759013285677 23.60285981036435 86-87 22.973480842840377 26.495933846734427 26.92102933187358 23.60955597855161 88-89 22.787873149165065 26.629101177850117 26.68294931881582 23.900076354168995 90-91 23.002190529032106 26.46957184595105 26.80501851058046 23.723219114436382 92-93 23.202974144783205 26.3318432230707 26.873197019227135 23.59198561291896 94-95 22.908412848744575 26.258571845045793 26.94504439520562 23.887970911004015 96-97 22.674928295024227 26.229884676546998 27.16159627004671 23.933590758382064 98-99 22.711575744520438 26.31924796082801 26.64946126091045 24.319715033741097 100-101 22.883409660040638 25.562042117429208 27.33984854792841 24.214699674601743 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 487.0 1 335.5 2 248.0 3 318.0 4 268.5 5 252.0 6 443.5 7 1023.5 8 1681.0 9 2049.5 10 1904.0 11 1556.0 12 1565.0 13 1703.0 14 1819.5 15 1933.0 16 2059.5 17 2179.0 18 2241.0 19 2427.5 20 2713.5 21 3106.0 22 3685.0 23 4365.5 24 5277.5 25 6503.0 26 8205.5 27 10240.0 28 12314.0 29 14778.5 30 17561.5 31 20654.5 32 24430.5 33 27803.5 34 30684.5 35 33692.5 36 37945.5 37 42076.0 38 44761.5 39 47350.5 40 50050.0 41 54132.0 42 57454.0 43 60617.0 44 65460.5 45 69060.0 46 71392.5 47 77782.0 48 88279.5 49 87351.0 50 78529.5 51 73640.5 52 70274.0 53 69432.5 54 69179.5 55 65794.5 56 60600.0 57 55929.0 58 51164.5 59 45011.5 60 38168.0 61 31655.0 62 25952.5 63 20638.5 64 16309.0 65 13166.0 66 10052.0 67 8072.5 68 6106.0 69 4097.5 70 2876.5 71 2028.0 72 1610.0 73 1336.0 74 1046.0 75 857.0 76 633.5 77 392.0 78 248.5 79 157.0 80 98.5 81 62.0 82 36.5 83 20.0 84 7.0 85 4.0 86 4.5 87 4.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29122136396612014 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001527565831614606 16-17 8.544012278522373E-4 18-19 7.767283889565793E-5 20-21 2.589094629855264E-5 22-23 0.0 24-25 0.0 26-27 5.178189259710528E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0013722201538232902 36-37 0.007094119285803424 38-39 0.002692658415049475 40-41 0.0029256769317364487 42-43 2.848004092840791E-4 44-45 0.0011133106908377637 46-47 1.0356378519421057E-4 48-49 3.6247324817973705E-4 50-51 7.767283889565793E-5 52-53 5.178189259710528E-5 54-55 0.0 56-57 7.508374426580267E-4 58-59 0.001734693402003027 60-61 5.696008185681582E-4 62-63 9.320740667478952E-4 64-65 0.0 66-67 4.1425514077684226E-4 68-69 0.0012686563686290795 70-71 0.0 72-73 0.0 74-75 5.178189259710528E-5 76-77 0.2562944774093726 78-79 1.5534567779131587E-4 80-81 1.5534567779131587E-4 82-83 0.0 84-85 0.0 86-87 0.10775811849457612 88-89 0.3753151575438191 90-91 0.27892316447430765 92-93 0.0 94-95 0.0 96-97 0.0 98-99 5.178189259710528E-5 100-101 2.589094629855265E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1931177.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.2135284242501 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6339768277969 43.640797630969345 2 13.035951686138189 14.655936812917602 3 3.975094368286918 6.703622407823195 4 1.6933618551820981 3.8075937911527906 5 0.9384259670345905 2.6376117385976667 6 0.5692683738001697 1.9200350346985484 7 0.37697481824506207 1.4833759262451718 8 0.28536162205646115 1.2832946922128794 9 0.20344244183316595 1.029259573801877 >10 1.1509772075253282 11.974049621802084 >50 0.08962971309508591 3.4692388924748077 >100 0.044120850657475646 4.536285580643226 >500 0.0018455504587295553 0.6589357524854088 >1k 0.001291885321110688 1.2824426205382 >5k 2.768325688094331E-4 0.9175199236370862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6156 0.31876933082778014 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6086 0.3151445983459828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5446 0.2820041870838354 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3299 0.17082846367785035 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3273 0.16948213447032562 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2120 0.1097776123058632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.178189259710529E-5 2 0.0 0.0 0.0 0.0 5.178189259710529E-5 3 0.0 0.0 0.0 0.0 5.178189259710529E-5 4 0.0 0.0 0.0 0.0 5.178189259710529E-5 5 0.0 0.0 0.0 0.0 5.178189259710529E-5 6 0.0 0.0 0.0 0.0 5.178189259710529E-5 7 0.0 0.0 0.0 0.0 5.178189259710529E-5 8 0.0 0.0 0.0 0.0 5.178189259710529E-5 9 0.0 0.0 0.0 0.0 5.178189259710529E-5 10-11 1.0356378519421058E-4 5.178189259710529E-5 0.0 0.0 5.178189259710529E-5 12-13 1.5534567779131587E-4 5.178189259710529E-5 0.0 0.0 1.0356378519421058E-4 14-15 1.8123662408986853E-4 5.178189259710529E-5 0.0 5.178189259710529E-5 1.294547314927632E-4 16-17 2.0712757038842116E-4 5.178189259710529E-5 0.0 5.178189259710529E-5 1.5534567779131587E-4 18-19 2.0712757038842116E-4 5.178189259710529E-5 0.0 5.178189259710529E-5 1.5534567779131587E-4 20-21 2.0712757038842116E-4 5.178189259710529E-5 0.0 5.178189259710529E-5 1.5534567779131587E-4 22-23 2.0712757038842116E-4 5.178189259710529E-5 0.0 1.294547314927632E-4 1.5534567779131587E-4 24-25 2.0712757038842116E-4 5.178189259710529E-5 0.0 4.142551407768423E-4 1.5534567779131587E-4 26-27 2.0712757038842116E-4 5.178189259710529E-5 0.0 6.213827111652635E-4 2.0712757038842116E-4 28-29 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.001242765422330527 2.0712757038842116E-4 30-31 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.0033399320725132913 2.0712757038842116E-4 32-33 2.0712757038842116E-4 5.178189259710529E-5 0.0 0.008440448493328163 2.589094629855264E-4 34-35 2.330185166869738E-4 5.178189259710529E-5 0.0 0.017139806449641852 2.589094629855264E-4 36-37 2.589094629855264E-4 5.178189259710529E-5 0.0 0.03130215407495015 2.589094629855264E-4 38-39 2.589094629855264E-4 5.178189259710529E-5 0.0 0.05227382057677779 2.589094629855264E-4 40-41 2.8480040928407905E-4 5.178189259710529E-5 0.0 0.07485072574911569 2.589094629855264E-4 42-43 3.1069135558263174E-4 5.178189259710529E-5 0.0 0.10068989015507124 2.589094629855264E-4 44-45 3.1069135558263174E-4 5.178189259710529E-5 0.0 0.1340115380413085 2.589094629855264E-4 46-47 3.3658230188118437E-4 5.178189259710529E-5 0.0 0.1752299245486043 2.589094629855264E-4 48-49 3.62473248179737E-4 5.178189259710529E-5 0.0 0.22227377397307446 2.589094629855264E-4 50-51 3.62473248179737E-4 5.178189259710529E-5 0.0 0.27283879209414774 2.589094629855264E-4 52-53 3.62473248179737E-4 5.178189259710529E-5 0.0 0.3245430118523574 2.589094629855264E-4 54-55 3.62473248179737E-4 5.178189259710529E-5 0.0 0.38686251959297363 2.589094629855264E-4 56-57 3.62473248179737E-4 5.178189259710529E-5 0.0 0.4643023399719446 2.589094629855264E-4 58-59 3.62473248179737E-4 5.178189259710529E-5 0.0 0.5497424627571683 2.589094629855264E-4 60-61 3.62473248179737E-4 5.178189259710529E-5 0.0 0.6441667439079898 2.589094629855264E-4 62-63 3.62473248179737E-4 5.178189259710529E-5 0.0 0.7412319015812636 2.589094629855264E-4 64-65 3.62473248179737E-4 5.178189259710529E-5 0.0 0.8518639151149792 3.1069135558263174E-4 66-67 3.62473248179737E-4 5.178189259710529E-5 0.0 0.9750789285497912 3.3658230188118437E-4 68-69 3.62473248179737E-4 1.0356378519421058E-4 0.0 1.119938773090193 3.62473248179737E-4 70-71 3.883641944782897E-4 1.0356378519421058E-4 0.0 1.2801260578393383 3.62473248179737E-4 72-73 4.4014608707539495E-4 1.0356378519421058E-4 0.0 1.4455174227944927 3.883641944782897E-4 74-75 4.660370333739476E-4 1.0356378519421058E-4 0.0 1.6221713493895173 4.142551407768423E-4 76-77 4.660370333739476E-4 1.294547314927632E-4 0.0 1.8211950535864916 4.142551407768423E-4 78-79 4.660370333739476E-4 1.5534567779131587E-4 0.0 2.051909276052894 4.142551407768423E-4 80-81 4.919279796725002E-4 1.5534567779131587E-4 0.0 2.3100161197031657 4.142551407768423E-4 82-83 5.178189259710528E-4 1.5534567779131587E-4 0.0 2.568459545655318 4.142551407768423E-4 84-85 5.178189259710528E-4 1.5534567779131587E-4 0.0 2.843939214271918 4.142551407768423E-4 86-87 5.437098722696056E-4 1.5534567779131587E-4 0.0 3.137076508264131 4.142551407768423E-4 88-89 5.696008185681582E-4 1.5534567779131587E-4 0.0 3.4579688966883926 4.142551407768423E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 6345 0.0 21.877295 12-13 GTACATG 7820 0.0 20.9369 1 GTACTTT 6725 0.0 20.49997 14-15 TACATGG 7925 0.0 20.418356 2 GTATCAA 14925 0.0 19.962765 1 AGTACTT 6625 0.0 19.269293 12-13 ACATGGG 8075 0.0 19.157581 3 CGTAAGA 200 1.296443E-5 18.981285 2 ATTCGCG 440 0.0 18.892025 80-81 AGAGTAC 11420 0.0 18.533716 10-11 TATAGCG 185 1.53354E-4 17.95527 3 GTGTAAG 750 0.0 17.768772 1 GGTATCA 12145 0.0 17.713387 1 CGCGGAA 445 0.0 17.687979 82-83 ATCAACG 16885 0.0 17.621063 3 CAACGCA 16945 0.0 17.61468 5 TCAACGC 16925 0.0 17.579418 4 TATCAAC 17045 0.0 17.455656 2 ACTTTTT 7980 0.0 17.364283 16-17 AACGCAG 17275 0.0 17.360598 6 >>END_MODULE