FastQCFastQC Report
Fri 27 May 2016
SRR939258_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939258_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960741
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58850.3001416301286095No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55980.285504306790137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47910.24434639761192326No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36550.1864091177774117No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30410.15509442603587112No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23590.12031165768451825No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC21140.10781638166387096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81000.022.636991
GTATCAA131150.021.8044221
TACATGG82650.021.5739042
GAGTACT61100.020.70983312-13
ACTTGAC9650.020.6261168
ACATGGG84050.020.5939143
GGTATCA104800.019.4905261
GTACTTT64600.019.47774114-15
GTCCAAG13250.019.3595091
AGTACTT65000.019.21205512-13
AGAGTAC110850.019.11053510-11
CATGGGG52000.018.7865754
ATCAACG152250.018.7509373
TCAACGC152900.018.7332534
TATCAAC153900.018.5807172
CAACGCA154350.018.4953485
CGGTTAC5050.018.33082428-29
AACGCAG157350.018.1612636
GTATAGA5350.017.7579421
GTATAGG4900.017.4498961
Produced by FastQC (version 0.11.5)