##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939258_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1960741 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.667189598218226 33.0 31.0 34.0 30.0 34.0 2 31.757934372770293 33.0 31.0 34.0 30.0 34.0 3 31.806782741830766 34.0 31.0 34.0 30.0 34.0 4 35.30116216267217 37.0 35.0 37.0 33.0 37.0 5 35.332984825634796 37.0 35.0 37.0 33.0 37.0 6 35.418862052662746 37.0 35.0 37.0 33.0 37.0 7 35.32898276722933 37.0 35.0 37.0 33.0 37.0 8 35.309878765221924 37.0 35.0 37.0 33.0 37.0 9 36.90727689174654 39.0 37.0 39.0 33.0 39.0 10-11 36.80109331115124 39.0 37.0 39.0 33.0 39.0 12-13 36.681577016036286 39.0 37.0 39.0 32.0 39.0 14-15 37.873914759777044 40.0 38.0 41.0 32.5 41.0 16-17 37.79289743010423 40.0 38.0 41.0 32.0 41.0 18-19 37.708903419676545 40.0 38.0 41.0 32.0 41.0 20-21 37.5949832231794 40.0 37.5 41.0 32.0 41.0 22-23 37.51163641704845 40.0 37.5 41.0 32.0 41.0 24-25 37.53098955955937 40.0 37.0 41.0 32.0 41.0 26-27 37.43622589623005 40.0 37.0 41.0 31.5 41.0 28-29 37.28062706905195 40.0 37.0 41.0 31.0 41.0 30-31 37.09730428445164 40.0 37.0 41.0 30.5 41.0 32-33 36.88265354781687 40.0 36.5 41.0 30.0 41.0 34-35 36.55743058364159 39.5 36.0 41.0 30.0 41.0 36-37 36.29084310472419 39.0 35.5 41.0 30.0 41.0 38-39 35.94916972715927 39.0 35.0 40.5 28.0 41.0 40-41 35.812876101433076 39.0 35.0 40.5 27.5 41.0 42-43 35.627769807435044 39.0 35.0 40.5 27.0 41.0 44-45 35.58275697810164 39.0 35.0 41.0 26.5 41.0 46-47 35.37421643144097 39.0 35.0 41.0 25.5 41.0 48-49 35.15745424816434 39.0 35.0 41.0 24.5 41.0 50-51 34.02129628543494 38.0 33.5 40.0 23.5 40.5 52-53 34.13238566439932 38.0 33.5 39.5 23.0 40.5 54-55 34.59117573407197 38.0 34.0 40.0 23.0 41.0 56-57 34.454549325994606 38.0 34.0 40.0 23.0 41.0 58-59 34.21782759681162 38.0 34.0 40.0 22.5 41.0 60-61 33.890184884184094 38.0 34.0 40.0 22.0 41.0 62-63 33.513003502247365 37.0 33.0 40.0 20.5 41.0 64-65 33.11599211726587 36.5 33.0 39.5 19.0 41.0 66-67 32.723221475962404 36.0 32.5 39.0 17.5 41.0 68-69 32.295855750453526 35.5 32.0 39.0 14.0 40.5 70-71 31.8587888456456 35.0 31.5 38.5 10.5 40.0 72-73 31.40345053222226 35.0 31.0 37.5 7.5 40.0 74-75 30.943002925934636 35.0 31.0 37.0 7.0 39.0 76-77 30.494397271235723 35.0 30.5 36.5 2.0 39.0 78-79 30.051873245880003 34.5 30.0 36.0 2.0 38.5 80-81 29.57769639131328 34.0 29.5 35.5 2.0 37.0 82-83 29.12700122045696 34.0 29.0 35.0 2.0 37.0 84-85 28.689866994161903 34.0 29.0 35.0 2.0 36.0 86-87 28.349861353437298 34.0 29.0 35.0 2.0 36.0 88-89 27.911551551173766 34.0 27.5 35.0 2.0 35.5 90-91 27.481217815101537 34.0 26.5 35.0 2.0 35.0 92-93 27.03823732966261 33.5 25.5 35.0 2.0 35.0 94-95 26.532769243872597 33.0 25.0 35.0 2.0 35.0 96-97 25.916721535378716 33.0 23.5 35.0 2.0 35.0 98-99 25.13177467090248 32.5 18.5 35.0 2.0 35.0 100-101 22.595305805305237 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27192361243114505 1101 2 0.089844106177285 1101 3 0.3022266524347117 1101 4 0.37246430560551147 1101 5 0.4244914345184938 1101 6 0.4326257387242691 1101 7 0.4474891290617933 1101 8 0.3993640437183643 1101 9 0.4157271287603379 1101 10-11 0.46411001051579603 1101 12-13 0.497868776825797 1101 14-15 0.4772067399037425 1101 16-17 0.5751682796216429 1101 18-19 0.6015651944895595 1101 20-21 0.684562672620693 1101 22-23 0.6410043196755666 1101 24-25 0.5764808373774031 1101 26-27 0.566015599051326 1101 28-29 0.61199687224358 1101 30-31 0.6986008094723672 1101 32-33 0.6875325813936826 1101 34-35 0.5566445615601907 1101 36-37 0.46664896595601846 1101 38-39 0.6019978318729144 1101 40-41 0.4527761491690754 1101 42-43 0.5514859167181783 1101 44-45 0.3253778304293391 1101 46-47 0.4095566098190915 1101 48-49 0.44347190707595985 1101 50-51 0.458738793799391 1101 52-53 0.4857283678049171 1101 54-55 0.4578978842058916 1101 56-57 0.6040873099676958 1101 58-59 0.4416046593063214 1101 60-61 0.6373018132278148 1101 62-63 0.6412718179968024 1101 64-65 0.4980169343092484 1101 66-67 0.3881459167700072 1101 68-69 0.4079552019883863 1101 70-71 0.36274543729324193 1101 72-73 0.4091409978404883 1101 74-75 0.3718845246268039 1101 76-77 0.31007535217313986 1101 78-79 0.17219840713471868 1101 80-81 0.08644291112975822 1101 82-83 0.19657303906386758 1101 84-85 0.1722574522014888 1101 86-87 0.07302000422432542 1101 88-89 0.028474803469311638 1101 90-91 0.3423304095977677 1101 92-93 0.32364383598877566 1101 94-95 0.366171745843932 1101 96-97 0.23978918519470227 1101 98-99 0.2171673169672097 1101 100-101 0.37694003193108827 1102 1 0.32542455204714216 1102 2 0.31600570024328434 1102 3 0.34868544356155695 1102 4 0.35786048549474714 1102 5 0.2538628861009258 1102 6 0.3258711922754216 1102 7 0.41830883216302084 1102 8 0.43616069888426523 1102 9 0.36987237649083937 1102 10-11 0.35623935934699347 1102 12-13 0.377459969292957 1102 14-15 0.6475506691889379 1102 16-17 0.6825852802082792 1102 18-19 0.6102106559951324 1102 20-21 0.6281086541465513 1102 22-23 0.5249431004616554 1102 24-25 0.6268696097119317 1102 26-27 0.6336499293387803 1102 28-29 0.6537526118556087 1102 30-31 0.5952759857125187 1102 32-33 0.6711247234198794 1102 34-35 0.7220803644098623 1102 36-37 0.7990561478040306 1102 38-39 0.8714370700382261 1102 40-41 0.8439963325184792 1102 42-43 0.7478768466894437 1102 44-45 0.8585595740384591 1102 46-47 0.7927757640652615 1102 48-49 0.8913225976191441 1102 50-51 0.6505602816738758 1102 52-53 0.7225389432078586 1102 54-55 0.7171038838280595 1102 56-57 0.760836014888497 1102 58-59 0.6621683294762448 1102 60-61 0.5974147411508142 1102 62-63 0.6308937841308477 1102 64-65 0.6958707320482063 1102 66-67 0.6060579060713067 1102 68-69 0.5717261693454248 1102 70-71 0.5216207069978296 1102 72-73 0.47945808095424525 1102 74-75 0.31254267840564864 1102 76-77 0.3545237876379268 1102 78-79 0.16130520021039274 1102 80-81 0.07039113779098471 1102 82-83 0.1666035144399629 1102 84-85 0.0639051856886077 1102 86-87 -0.08153502048409678 1102 88-89 -0.17030369573027215 1102 90-91 -0.18798463519556563 1102 92-93 -0.2145945580135944 1102 94-95 -0.09199483464983516 1102 96-97 -0.4315248106093392 1102 98-99 -0.3792390246950639 1102 100-101 -0.3985302732203948 1103 1 0.26649062741319796 1103 2 0.2880531199098151 1103 3 0.3453953015998579 1103 4 0.32098141307694306 1103 5 0.3268783953544485 1103 6 0.17281083997256985 1103 7 0.20009536933815752 1103 8 0.34389798303063657 1103 9 0.374755561586106 1103 10-11 0.48686270250780694 1103 12-13 0.46015761317473647 1103 14-15 0.5187160929681411 1103 16-17 0.5624944792271833 1103 18-19 0.5573237298499052 1103 20-21 0.6346257613982473 1103 22-23 0.6083059575337231 1103 24-25 0.6066642235909825 1103 26-27 0.5711548843718575 1103 28-29 0.5304788215030527 1103 30-31 0.5513629341553994 1103 32-33 0.506500252989035 1103 34-35 0.325737798198233 1103 36-37 0.5084697815775385 1103 38-39 0.6362498317259053 1103 40-41 0.7089744684350379 1103 42-43 0.5266470074802712 1103 44-45 0.42191989165608135 1103 46-47 0.31701578586740453 1103 48-49 0.3029251172534657 1103 50-51 0.262488594732865 1103 52-53 0.3989653733542795 1103 54-55 0.45868404387562833 1103 56-57 0.4410254829656992 1103 58-59 0.1707783188229257 1103 60-61 0.22409604581102371 1103 62-63 0.2038708918780472 1103 64-65 0.17376756825958495 1103 66-67 0.08713680258544088 1103 68-69 0.22541364302181677 1103 70-71 -0.008238012621621493 1103 72-73 -0.07709309214983406 1103 74-75 -0.028192958192224893 1103 76-77 0.06309258827216979 1103 78-79 0.06058517196670721 1103 80-81 -0.026075923626649455 1103 82-83 -0.1598414970804889 1103 84-85 -0.20636678368928685 1103 86-87 -0.1445391950994619 1103 88-89 -0.13661566461266617 1103 90-91 -0.12235212522356065 1103 92-93 0.023735100336878645 1103 94-95 -0.20873943985757748 1103 96-97 -0.3204388531730764 1103 98-99 -0.22606656476889597 1103 100-101 -0.4023518424793693 1104 1 0.48983053437873636 1104 2 0.4238593432828388 1104 3 0.3570125656641707 1104 4 0.38030217120240195 1104 5 0.38897212230283174 1104 6 0.3589459958579795 1104 7 0.3708075797345316 1104 8 0.46655744379206965 1104 9 0.4848538649868317 1104 10-11 0.47697590948689594 1104 12-13 0.6082254169292298 1104 14-15 0.44220127218743954 1104 16-17 0.5415963504542489 1104 18-19 0.5259622352769142 1104 20-21 0.5773455891791102 1104 22-23 0.6014307411307627 1104 24-25 0.6991047974540123 1104 26-27 0.6657308850944545 1104 28-29 0.5800279506222381 1104 30-31 0.5976001569714242 1104 32-33 0.6739336761452819 1104 34-35 0.8542668601760397 1104 36-37 0.9394978274022776 1104 38-39 0.8469006213144823 1104 40-41 0.7706496908596563 1104 42-43 0.7146819932541248 1104 44-45 0.8023510416402786 1104 46-47 0.6644189502433377 1104 48-49 0.6186235531304405 1104 50-51 0.6644064895647901 1104 52-53 0.6005084206091666 1104 54-55 0.6543657696798988 1104 56-57 0.6532336212227321 1104 58-59 0.7311536180235549 1104 60-61 0.5132804136132734 1104 62-63 0.39757826990204137 1104 64-65 0.5238450969753146 1104 66-67 0.47846186618910025 1104 68-69 0.5413424069280595 1104 70-71 0.5016299627498455 1104 72-73 0.33678906420225374 1104 74-75 0.4775750277171191 1104 76-77 0.7302204128013514 1104 78-79 0.5166926282721249 1104 80-81 0.5657406569806369 1104 82-83 0.450031747232817 1104 84-85 0.48673466602242854 1104 86-87 0.516957636008371 1104 88-89 0.31183300836669403 1104 90-91 0.5070591081604547 1104 92-93 0.49745136029141435 1104 94-95 0.4787724152615773 1104 96-97 0.313776902468458 1104 98-99 0.24488274801054644 1104 100-101 -0.04845207280740027 1105 1 0.1975328294780816 1105 2 0.13087065022790156 1105 3 0.3715006470753508 1105 4 0.34125147763813146 1105 5 0.32094909328986176 1105 6 0.21639546348502137 1105 7 0.29434784149745497 1105 8 0.2489500528390991 1105 9 0.23047130132361815 1105 10-11 0.34728770795506847 1105 12-13 0.3712625557024367 1105 14-15 0.38533753993872466 1105 16-17 0.3941255820206564 1105 18-19 0.4358330319741839 1105 20-21 0.4696668471485168 1105 22-23 0.4936380611301132 1105 24-25 0.42477911445182315 1105 26-27 0.4946756928940488 1105 28-29 0.46831412629574487 1105 30-31 0.6099076658369427 1105 32-33 0.351370130701838 1105 34-35 0.49647543143217376 1105 36-37 0.5289753467341924 1105 38-39 0.7027827794232877 1105 40-41 0.7088801488049157 1105 42-43 0.7030665029896355 1105 44-45 0.5757229725650177 1105 46-47 0.6904004875815772 1105 48-49 0.5526187276954815 1105 50-51 0.5321221510306415 1105 52-53 0.501852270387694 1105 54-55 0.4962445979736003 1105 56-57 0.5089882983469636 1105 58-59 0.5978088025692045 1105 60-61 0.6996181616749269 1105 62-63 0.7518249617999331 1105 64-65 0.6944068523855336 1105 66-67 0.7309475245783332 1105 68-69 0.5424941519896436 1105 70-71 0.4607499317775563 1105 72-73 0.6148015384962839 1105 74-75 0.6478082178183548 1105 76-77 0.5740026234629454 1105 78-79 0.610120438852455 1105 80-81 0.6334015575616618 1105 82-83 0.6071452892168985 1105 84-85 0.6495162539997636 1105 86-87 0.5553033567837957 1105 88-89 0.4471452410945673 1105 90-91 0.3170698984100717 1105 92-93 0.5436387808918113 1105 94-95 0.4254465011448971 1105 96-97 0.5194038777980872 1105 98-99 0.5385335767105524 1105 100-101 0.3376946009840722 1106 1 0.1759064653505824 1106 2 0.20342991548992373 1106 3 0.280235817253633 1106 4 0.28685839281911285 1106 5 0.2232246200321626 1106 6 0.23033128196151864 1106 7 0.36290494576541477 1106 8 0.24150240292013336 1106 9 0.29863769786602745 1106 10-11 0.24597209477300197 1106 12-13 0.301934408754839 1106 14-15 0.4491949143309455 1106 16-17 0.4229718272934875 1106 18-19 0.35299002819244407 1106 20-21 0.36706112051426487 1106 22-23 0.4135317402224956 1106 24-25 0.3120690118046099 1106 26-27 0.3781000040771971 1106 28-29 0.32071823218445417 1106 30-31 0.3798417555181999 1106 32-33 0.35824684599087675 1106 34-35 0.6544376140361692 1106 36-37 0.4664616784467839 1106 38-39 0.6156177875270146 1106 40-41 0.5676691277352219 1106 42-43 0.42521496328136976 1106 44-45 0.582899633829193 1106 46-47 0.627874717727444 1106 48-49 0.7023903106236347 1106 50-51 0.6096620753958533 1106 52-53 0.37862504186256984 1106 54-55 0.5147893845754439 1106 56-57 0.4435028310319922 1106 58-59 0.5121919468155554 1106 60-61 0.593961975829437 1106 62-63 0.5841257721727047 1106 64-65 0.552658175994388 1106 66-67 0.5189298044270672 1106 68-69 0.42071824707338124 1106 70-71 0.45287137964356816 1106 72-73 0.4903905174265937 1106 74-75 0.43171054088696437 1106 76-77 0.41080824204749433 1106 78-79 0.3780724647843883 1106 80-81 0.4927277596146027 1106 82-83 0.338721302182865 1106 84-85 0.4222129584838257 1106 86-87 0.38506154154879013 1106 88-89 0.30458359873908236 1106 90-91 0.2833657384965136 1106 92-93 0.350775247666526 1106 94-95 0.27649739255494765 1106 96-97 0.3187309442486601 1106 98-99 0.191479659908822 1106 100-101 0.013091251443281493 1107 1 -0.03611903707491848 1107 2 -0.02909232983232357 1107 3 0.01880934462716155 1107 4 -0.12529074093001924 1107 5 -0.10648154767400086 1107 6 -0.20214522531498602 1107 7 -0.038297826237354116 1107 8 -0.14526765944992803 1107 9 -0.027708802480468364 1107 10-11 -0.012684454083547791 1107 12-13 -0.015459770039335297 1107 14-15 -0.012066346930318161 1107 16-17 -0.007623598901936646 1107 18-19 0.07269772050013756 1107 20-21 -0.048657798404654784 1107 22-23 -0.05737442748367272 1107 24-25 -0.1490188606832703 1107 26-27 -0.03973173535454322 1107 28-29 0.023143734609959665 1107 30-31 -0.0277563040798654 1107 32-33 -0.1393230777790464 1107 34-35 -0.095811173212617 1107 36-37 -0.11239805821295334 1107 38-39 0.09286186237109462 1107 40-41 -0.04390828044218864 1107 42-43 -0.042936388069982456 1107 44-45 0.15766609259565456 1107 46-47 0.12344547309819376 1107 48-49 0.3508036162369379 1107 50-51 0.32970698184075076 1107 52-53 0.1911231707606973 1107 54-55 0.14836512955118764 1107 56-57 0.18421301814218083 1107 58-59 0.162543575367188 1107 60-61 0.025270222537685072 1107 62-63 0.28918578603271783 1107 64-65 0.3679093194455376 1107 66-67 0.2594921051893593 1107 68-69 0.15795289710803218 1107 70-71 0.07683021540240631 1107 72-73 0.04808379531987228 1107 74-75 0.14251608489250955 1107 76-77 -0.054413240973989474 1107 78-79 -0.09853917762725573 1107 80-81 -0.21016926048241658 1107 82-83 -0.19204765138609048 1107 84-85 -0.32524636237549487 1107 86-87 -0.3186863447990973 1107 88-89 -0.2754622779067937 1107 90-91 -0.23147819774742473 1107 92-93 -0.1535891320978493 1107 94-95 -0.2997767585192044 1107 96-97 -0.27801486309715173 1107 98-99 -0.11005020842104685 1107 100-101 0.025398527950560634 1108 1 0.24290221003143486 1108 2 0.23649334000866773 1108 3 0.30450572606822846 1108 4 0.3058241476316752 1108 5 0.31781023300075617 1108 6 0.23192966088755895 1108 7 0.30184071070969054 1108 8 0.3190780868107126 1108 9 0.35840264214161266 1108 10-11 0.3080819225338942 1108 12-13 0.3306343074377196 1108 14-15 0.3910388859621534 1108 16-17 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-1.989593834871627 2316 52-53 -1.9886809115455435 2316 54-55 -1.704282458967839 2316 56-57 -1.8745537694856935 2316 58-59 -1.4699266611798407 2316 60-61 -1.5065616940008795 2316 62-63 -1.6846253351036822 2316 64-65 -1.7491181829357174 2316 66-67 -1.602062545299713 2316 68-69 -1.6220069758199145 2316 70-71 -1.5058009240665235 2316 72-73 -1.9538891489524701 2316 74-75 -1.8010436725153944 2316 76-77 -1.508801881622336 2316 78-79 -1.4967875869843823 2316 80-81 -1.2866353695197077 2316 82-83 -1.1206328536986447 2316 84-85 -1.213583187619136 2316 86-87 -1.080922007487736 2316 88-89 -1.1566176667196437 2316 90-91 -1.1383820900913513 2316 92-93 -1.180029814168389 2316 94-95 -1.3511760040513217 2316 96-97 -1.0631697747619846 2316 98-99 -1.1092066788889916 2316 100-101 -0.6895254871244489 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8663.0 3 2764.0 4 2865.0 5 3076.0 6 3451.0 7 4186.0 8 4976.0 9 5910.0 10 6970.0 11 8328.0 12 9873.0 13 12526.0 14 11941.0 15 12389.0 16 14720.0 17 17922.0 18 16141.0 19 13067.0 20 13621.0 21 14232.0 22 15373.0 23 16987.0 24 19030.0 25 21730.0 26 24388.0 27 28266.0 28 32954.0 29 38541.0 30 46123.0 31 56055.0 32 69310.0 33 88622.0 34 119498.0 35 170013.0 36 259853.0 37 386022.0 38 332156.0 39 48186.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20952169559173 18.71372006697755 15.36335935174568 22.71339888568503 2 15.393110250994436 20.063676544393612 39.6429886110273 24.90022459358466 3 19.631535330728216 24.019390834899625 29.38179203870814 26.96728179566402 4 13.25187331656816 16.59507001799916 36.93478177617218 33.2182748892605 5 14.528387829468764 36.79004894030753 33.43888385051555 15.242679379708154 6 32.027972527565055 35.412527932469125 17.434708571026164 15.124790968939655 7 28.99914875896406 32.19676324271801 20.30822274344575 18.495865254872182 8 26.794698404111443 33.894999004802465 19.471881841983045 19.838420749103047 9 26.413776103612697 17.456062948244178 20.348863023367258 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23.42721245418811 25.962899590357736 27.90252732865497 22.707360626799183 36-37 23.178790828236615 25.75323379366527 28.196356877837193 22.871618500260926 38-39 22.89050274641206 26.10329825308338 28.189000432916828 22.81719856758773 40-41 23.18012073435219 25.992318393237486 28.079741565658196 22.747819306752127 42-43 22.93084751695561 26.00967312889539 27.667185412213364 23.392293941935637 44-45 23.1521211455847 25.948701885465585 27.697972351008758 23.201204617940956 46-47 23.36592299585348 26.419661900806013 27.24297699682702 22.971438106513478 48-49 23.40570662759799 26.296824697220302 27.204639098471805 23.09282957670991 50-51 22.88744875815384 26.511367327095805 27.481958340062352 23.119225574688006 52-53 23.332079247958273 26.62953038341741 26.97547879188069 23.062911576743627 54-55 23.093601913320207 26.272121997194304 27.17051345501594 23.46376263446955 56-57 23.257015214587625 26.464949958848035 27.185448397836304 23.09258642872804 58-59 23.641650376311052 26.12293458669735 27.006962432333847 23.228452604657754 60-61 23.23641373272568 26.24297879216816 27.686903222903148 22.833704252203013 62-63 23.009150039148658 26.438239794482342 27.53992907184418 23.01268109452482 64-65 22.83634200609524 26.592539563136057 27.267740862962548 23.303377567806155 66-67 22.941176621154078 26.470456490183476 27.03892233884701 23.549444549815437 68-69 22.77616247070816 26.280406043654764 27.35697876651978 23.5864527191173 70-71 23.073829485868995 26.286121097016924 27.19533558147411 23.44471383563997 72-73 23.110480443678632 26.116670403575963 27.344091536873055 23.428757615872343 74-75 22.821106582605914 26.83202891736247 26.93257009406794 23.414294405963666 76-77 23.000930273029375 26.352881863002903 27.006683378311457 23.639504485656264 78-79 23.088940600937214 26.462908687581475 27.04419589115225 23.403954820329062 80-81 23.15008058229052 26.61154986392224 26.87797418245243 23.36039537133481 82-83 23.16390310659329 26.483117965179094 27.090045195837543 23.262933732390078 84-85 23.100268325670847 26.493711556941474 26.939462299411527 23.466557817976156 86-87 23.038316288120146 26.515452774190972 27.035253728272536 23.410977209416348 88-89 22.991423110509608 26.57008367022894 26.702043691154177 23.73644952810728 90-91 23.129689503958957 26.478459321153103 26.80352146388042 23.588329711007518 92-93 23.365162303686365 26.35266724416512 26.9217950371995 23.36037541494902 94-95 23.042897295304794 26.245501133995813 27.020099627803635 23.69150194289576 96-97 22.85510954557498 26.267924272309422 27.22502889514933 23.65193728696627 98-99 22.977417435096722 26.30084916587211 26.727390238751912 23.994343160279257 100-101 23.169062671460043 25.63125445284286 27.23914018163504 23.960542694062056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1594.0 1 925.5 2 290.5 3 347.0 4 324.5 5 330.0 6 548.0 7 1130.0 8 1869.0 9 2224.5 10 2003.0 11 1755.5 12 1829.0 13 1983.0 14 2149.5 15 2312.0 16 2473.0 17 2568.0 18 2634.0 19 2766.5 20 3048.0 21 3374.5 22 3848.5 23 4659.5 24 5562.0 25 6661.0 26 8333.5 27 10382.0 28 12422.5 29 14780.0 30 17776.0 31 21125.5 32 24739.5 33 28167.5 34 31337.0 35 34132.0 36 38114.0 37 42613.5 38 45814.0 39 48065.5 40 50541.0 41 54622.0 42 57817.0 43 61303.5 44 65820.0 45 69352.0 46 72092.0 47 78076.5 48 88568.0 49 87992.5 50 79324.0 51 74587.0 52 71037.0 53 70113.0 54 69645.0 55 66091.0 56 61308.0 57 56627.0 58 51533.0 59 45536.5 60 38771.5 61 32197.5 62 26524.5 63 21296.5 64 17049.0 65 13788.5 66 10472.0 67 8424.5 68 6475.0 69 4437.0 70 3261.5 71 2369.5 72 1779.0 73 1425.0 74 1149.0 75 978.0 76 695.5 77 443.0 78 310.0 79 199.0 80 131.5 81 101.0 82 78.5 83 48.0 84 29.5 85 23.5 86 17.5 87 13.0 88 10.0 89 6.0 90 6.5 91 5.5 92 5.0 93 5.5 94 3.0 95 2.5 96 2.0 97 2.0 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2784151501906677 2 0.10669435687834346 3 0.11679257994809106 4 0.11811860924007811 5 0.12428974556047943 6 0.05630524378283517 7 0.06370040714199376 8 0.0678824995244145 9 0.06579145333320413 10-11 0.061481858134246184 12-13 0.06533244319367015 14-15 0.061507358697553624 16-17 0.06171136320401318 18-19 0.06627596403604556 20-21 0.07022855134869929 22-23 0.0787967406200003 24-25 0.08662541355538544 26-27 0.08688041918845987 28-29 0.08035227498175435 30-31 0.07724120625824624 32-33 0.07030505303862163 34-35 0.23684923199953486 36-37 0.362847515301613 38-39 0.3343123849605838 40-41 0.3163089872655287 42-43 0.340075512268066 44-45 0.35325930349801427 46-47 0.35976194714141235 48-49 0.3701661769708493 50-51 0.36402054121375543 52-53 0.36343402825768417 54-55 0.34986772857812426 56-57 0.3263817097719689 58-59 0.31151488136372935 60-61 0.2670929000821628 62-63 0.3392084931156129 64-65 0.36973266739462274 66-67 0.3736087530173542 68-69 0.38516050819562603 70-71 0.38615503016461633 72-73 0.3700131735910046 74-75 0.37294573837136064 76-77 0.35769640151350945 78-79 0.33372587200451254 80-81 0.3329098539786744 82-83 0.3468076609812311 84-85 0.35489133954969065 86-87 0.3478276835135288 88-89 0.36307702037138 90-91 0.3863845352343833 92-93 0.38194743721888813 94-95 0.38102941693982023 96-97 0.3854920155186228 98-99 0.375036784562571 100-101 0.35359081082101107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1960741.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.357084185950455 #Duplication Level Percentage of deduplicated Percentage of total 1 79.46917124746192 46.37589116675848 2 11.90875191554321 13.89920076169908 3 3.6893323909709954 6.458960427895448 4 1.5633355492665872 3.649268170377573 5 0.8516349275048459 2.484946558004806 6 0.5380241279661395 1.883851159787555 7 0.3516391062677201 1.4364443049276405 8 0.2481808766345559 1.1586489848884611 9 0.19587074069029267 1.0287400773625093 >10 1.0584415829242708 11.32496885364777 >50 0.08118692307640885 3.2500757162004463 >100 0.041539603218246235 4.451777355136369 >500 0.0013140947612756068 0.4786786224165849 >1k 0.001401701078693978 1.5314852945755841 >5k 1.7521263483674725E-4 0.5870625463216529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5885 0.3001416301286095 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5598 0.285504306790137 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4791 0.24434639761192326 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3655 0.1864091177774117 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3041 0.15509442603587112 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2359 0.12031165768451825 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2114 0.10781638166387096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0200225322977385E-4 0.0 0.0 0.0 0.0 7 1.5300337984466077E-4 0.0 0.0 0.0 0.0 8 1.5300337984466077E-4 0.0 0.0 0.0 0.0 9 1.5300337984466077E-4 0.0 0.0 0.0 0.0 10-11 1.5300337984466077E-4 0.0 0.0 0.0 0.0 12-13 1.5300337984466077E-4 1.0200225322977385E-4 0.0 0.0 7.650168992233038E-5 14-15 1.5300337984466077E-4 1.275028165372173E-4 0.0 0.0 1.275028165372173E-4 16-17 1.7850394315210424E-4 1.5300337984466077E-4 0.0 0.0 2.040045064595477E-4 18-19 2.2950506976699114E-4 1.5300337984466077E-4 0.0 0.0 2.040045064595477E-4 20-21 2.550056330744346E-4 1.5300337984466077E-4 0.0 7.650168992233038E-5 2.550056330744346E-4 22-23 2.550056330744346E-4 1.5300337984466077E-4 0.0 1.5300337984466077E-4 2.550056330744346E-4 24-25 2.550056330744346E-4 1.5300337984466077E-4 0.0 2.550056330744346E-4 2.550056330744346E-4 26-27 2.550056330744346E-4 1.5300337984466077E-4 0.0 3.5700788630420847E-4 2.550056330744346E-4 28-29 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.0012240270387572862 2.550056330744346E-4 30-31 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.0035700788630420844 2.550056330744346E-4 32-33 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.008440686454763785 2.550056330744346E-4 34-35 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.01790139544182531 3.0600675968932154E-4 36-37 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.03128919117823313 3.0600675968932154E-4 38-39 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.05189364633064744 3.31507322996765E-4 40-41 2.550056330744346E-4 1.5300337984466077E-4 0.0 0.07331411950889996 3.5700788630420847E-4 42-43 2.8050619638187807E-4 1.5300337984466077E-4 0.0 0.09871268056311364 3.5700788630420847E-4 44-45 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.132143919059172 3.5700788630420847E-4 46-47 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.17416884738983884 3.5700788630420847E-4 48-49 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.21907533937424678 3.5700788630420847E-4 50-51 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.2707139800718198 3.5700788630420847E-4 52-53 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.3219956128830886 3.5700788630420847E-4 54-55 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.3825084496116519 3.5700788630420847E-4 56-57 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.45556756348747746 3.5700788630420847E-4 58-59 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.5422184776061703 3.5700788630420847E-4 60-61 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.6322099655181384 3.5700788630420847E-4 62-63 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.7276330734145917 3.5700788630420847E-4 64-65 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.8328483976211034 3.5700788630420847E-4 66-67 3.0600675968932154E-4 1.5300337984466077E-4 0.0 0.9562711240291297 3.8250844961165194E-4 68-69 3.0600675968932154E-4 1.5300337984466077E-4 0.0 1.098946775734276 4.080090129190954E-4 70-71 3.31507322996765E-4 1.5300337984466077E-4 0.0 1.257942787956186 4.080090129190954E-4 72-73 3.5700788630420847E-4 1.5300337984466077E-4 0.0 1.4247419725501738 4.335095762265388E-4 74-75 3.5700788630420847E-4 1.5300337984466077E-4 0.0 1.5988598188133976 4.590101395339823E-4 76-77 3.5700788630420847E-4 1.5300337984466077E-4 0.0 1.7986822328905245 4.590101395339823E-4 78-79 3.5700788630420847E-4 1.5300337984466077E-4 0.0 2.029819338709192 4.590101395339823E-4 80-81 3.5700788630420847E-4 1.5300337984466077E-4 0.0 2.2842894599541705 4.590101395339823E-4 82-83 3.5700788630420847E-4 1.5300337984466077E-4 0.0 2.551152854966566 4.590101395339823E-4 84-85 3.5700788630420847E-4 1.5300337984466077E-4 0.0 2.825768421224425 4.590101395339823E-4 86-87 3.5700788630420847E-4 1.5300337984466077E-4 0.0 3.113822784345306 4.590101395339823E-4 88-89 3.5700788630420847E-4 1.5300337984466077E-4 0.0 3.4354613893420907 4.590101395339823E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8100 0.0 22.63699 1 GTATCAA 13115 0.0 21.804422 1 TACATGG 8265 0.0 21.573904 2 GAGTACT 6110 0.0 20.709833 12-13 ACTTGAC 965 0.0 20.626116 8 ACATGGG 8405 0.0 20.593914 3 GGTATCA 10480 0.0 19.490526 1 GTACTTT 6460 0.0 19.477741 14-15 GTCCAAG 1325 0.0 19.359509 1 AGTACTT 6500 0.0 19.212055 12-13 AGAGTAC 11085 0.0 19.110535 10-11 CATGGGG 5200 0.0 18.786575 4 ATCAACG 15225 0.0 18.750937 3 TCAACGC 15290 0.0 18.733253 4 TATCAAC 15390 0.0 18.580717 2 CAACGCA 15435 0.0 18.495348 5 CGGTTAC 505 0.0 18.330824 28-29 AACGCAG 15735 0.0 18.161263 6 GTATAGA 535 0.0 17.757942 1 GTATAGG 490 0.0 17.449896 1 >>END_MODULE