FastQCFastQC Report
Fri 27 May 2016
SRR939258_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939258_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960741
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61970.31605398163245424No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61900.3156969737461501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54470.2778031366712891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33680.17177179443893914No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32950.1680487121960524No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21670.11051944137445996No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1201.7237075E-423.7249455
GTACATG80950.021.333751
TACATGG81400.021.1600862
GAGTACT65850.021.00745212-13
GTATCGA1150.003690380120.6847171
ACATGGG81400.020.2857023
GTATCAA153950.020.1177181
GTACTTT68500.019.91763914-15
AGTACTT69750.019.76480312-13
TGCACCG5850.019.4666215
AGAGTAC119400.019.25637210-11
TACACCG2905.2817995E-817.9982345
CATGGGG49850.017.9900284
GCACCGT6700.017.7051836
GTGTAAG7850.017.5754221
GTACACG3551.553417E-917.4217761
GGTATCA125700.017.4100481
ATCAACG177450.017.2739523
TCAACGC177800.017.239954
CAACGCA178250.017.1964265
Produced by FastQC (version 0.11.5)