FastQCFastQC Report
Fri 27 May 2016
SRR939257_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939257_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824942
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42460.1503039708425872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.11961307524189876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89900.030.9321331
GGTATCA61400.029.3688661
ATCAACG104800.026.0597883
TCAACGC106200.025.8943754
CAACGCA107400.025.384325
TATCAAC110100.025.0206432
AACGCAG110450.024.7533916
GTACATG89150.024.5919281
TACATGG88300.023.8951052
ACATGGG90500.022.1616173
ACGCAGA124250.021.622777
CGCAGAG126450.021.0966848
GCAGAGT136900.019.1741269
GAGTACT59150.019.10464712-13
CATGGGG55300.018.348084
AGAGTAC115550.017.8773310-11
GTACTTT65200.017.73378814-15
AGTACTT63600.017.65617412-13
GTGTAGC14750.017.3729551
GTATAGC12500.016.7037731
Produced by FastQC (version 0.11.5)