FastQCFastQC Report
Fri 27 May 2016
SRR939257_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939257_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2824942
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.15260490303871727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.12690526035578784No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59600.031.9494671
GTATCAA87850.030.0746841
ATCAACG100550.026.0045283
TCAACGC103100.025.3153254
CAACGCA104550.024.9642265
TATCAAC108800.024.3816092
AACGCAG109850.023.7597626
TAGCGCG1000.001663447823.7273624
GTACATG87550.023.0547071
TACATGG88100.022.3538152
ACATGGG89150.021.398543
ACGCAGA123550.021.0099017
CGCAGAG125050.020.7199368
GAGTACT59900.019.49064412-13
GGACCGA3455.2750693E-1119.2569896
CGCGGAA3850.018.58060882-83
GCAGAGT137700.018.576229
ATTCGCG3750.018.36866880-81
GTGTAGG12350.018.116771
CATGGGG53750.017.745864
Produced by FastQC (version 0.11.5)