FastQCFastQC Report
Fri 27 May 2016
SRR939256_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939256_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43060.1494194122396915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33960.11784215605341207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA89750.031.8079911
GGTATCA60650.030.6226081
ATCAACG105500.026.565713
TCAACGC107000.026.1932934
CAACGCA108400.025.8112555
TATCAAC111550.025.2524362
AACGCAG112000.025.0501886
GTACATG93050.024.4010071
TACATGG94050.023.2449442
ACGCAGA125350.022.2310687
ACATGGG94900.022.0373213
CGCAGAG127400.021.7989468
GCAGAGT137550.019.8802819
GAGTACT59750.019.67178212-13
CATGGGG56000.018.8844034
GTACTTT63350.018.51648314-15
AGTACTT62950.017.76831212-13
GTGTAGC16200.017.59271
AGAGTAC118950.017.53085310-11
ATTCGCG3300.017.2833680-81
Produced by FastQC (version 0.11.5)