##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939256_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2881821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.896213886983265 33.0 31.0 34.0 30.0 34.0 2 31.98237017496923 34.0 31.0 34.0 30.0 34.0 3 32.04257273439259 34.0 31.0 34.0 30.0 34.0 4 35.52727355377034 37.0 35.0 37.0 33.0 37.0 5 35.56450348581678 37.0 35.0 37.0 33.0 37.0 6 35.65190377889536 37.0 35.0 37.0 33.0 37.0 7 35.568291368547875 37.0 35.0 37.0 33.0 37.0 8 35.545836469371274 37.0 35.0 37.0 33.0 37.0 9 37.188572086885344 39.0 37.0 39.0 34.0 39.0 10-11 37.0954233451696 39.0 37.0 39.0 33.0 39.0 12-13 37.002922631211305 39.0 37.0 39.0 33.0 39.0 14-15 38.28013051469887 40.0 38.0 41.0 33.0 41.0 16-17 38.21473991618494 40.0 38.0 41.0 33.0 41.0 18-19 38.13346821332762 40.0 38.0 41.0 33.0 41.0 20-21 38.02233952074053 40.0 38.0 41.0 33.0 41.0 22-23 37.95143209796861 40.0 38.0 41.0 33.0 41.0 24-25 37.98587021886509 40.0 38.0 41.0 33.0 41.0 26-27 37.91962477891583 40.0 38.0 41.0 33.0 41.0 28-29 37.79129602428465 40.0 38.0 41.0 32.5 41.0 30-31 37.646530960805684 40.0 37.5 41.0 32.0 41.0 32-33 37.48264552170312 40.0 37.0 41.0 31.5 41.0 34-35 37.208404685787215 40.0 37.0 41.0 31.0 41.0 36-37 36.98221905524319 40.0 37.0 41.0 30.0 41.0 38-39 36.685175276327016 39.5 36.5 41.0 30.0 41.0 40-41 36.57626202321379 39.0 36.0 41.0 30.0 41.0 42-43 36.445834422054666 39.0 36.0 41.0 30.0 41.0 44-45 36.4820710585425 40.0 36.0 41.0 30.0 41.0 46-47 36.359911666963356 40.0 36.0 41.0 30.0 41.0 48-49 36.221570319600005 40.0 36.0 41.0 29.0 41.0 50-51 35.105279613133504 38.0 34.5 40.0 27.0 40.5 52-53 35.21763062313725 38.5 34.5 39.5 27.5 40.5 54-55 35.731257076688664 39.0 35.0 40.5 28.0 41.0 56-57 35.639681298734374 39.0 35.0 41.0 28.0 41.0 58-59 35.45045944907751 39.0 35.0 41.0 27.5 41.0 60-61 35.17369277966952 38.5 35.0 40.5 26.5 41.0 62-63 34.821125253789184 38.0 34.0 40.0 26.0 41.0 64-65 34.44956539632406 37.5 34.0 40.0 26.0 41.0 66-67 34.10312594016075 37.0 34.0 40.0 25.5 41.0 68-69 33.69797031113313 36.5 33.5 39.0 24.5 41.0 70-71 33.28916126296533 36.0 33.0 39.0 23.5 40.5 72-73 32.84525652356618 35.0 33.0 38.5 22.5 40.0 74-75 32.395820906295015 35.0 32.0 37.5 22.0 39.5 76-77 31.946670178335154 35.0 32.0 37.0 20.5 39.0 78-79 31.520543087166068 35.0 31.5 36.5 19.5 39.0 80-81 31.067297205482227 35.0 31.0 36.0 18.0 37.5 82-83 30.62920962127766 34.5 31.0 35.5 15.0 37.0 84-85 30.21976920148753 34.0 30.5 35.0 9.0 37.0 86-87 29.897472466194117 34.0 30.0 35.0 7.0 36.0 88-89 29.501282348903697 34.0 30.0 35.0 2.0 36.0 90-91 29.11709332397814 34.0 29.5 35.0 2.0 35.5 92-93 28.719677939747125 34.0 29.0 35.0 2.0 35.0 94-95 28.266215181303764 34.0 29.0 35.0 2.0 35.0 96-97 27.721984467459983 34.0 28.0 35.0 2.0 35.0 98-99 27.012039783178764 34.0 26.5 35.0 2.0 35.0 100-101 24.533001355739998 31.0 20.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3371740008147732 1101 2 0.11691891332058546 1101 3 0.28526713978448015 1101 4 0.35231824755801 1101 5 0.3295325086054035 1101 6 0.3976196750493841 1101 7 0.3889578263426614 1101 8 0.3223935849013344 1101 9 0.4205195336664218 1101 10-11 0.4280115772421098 1101 12-13 0.3785098995275291 1101 14-15 0.4860909165400713 1101 16-17 0.5248881091672146 1101 18-19 0.5910104164978378 1101 20-21 0.5378386144637446 1101 22-23 0.4759087841178413 1101 24-25 0.475949321052191 1101 26-27 0.41024363493919935 1101 28-29 0.4707215054136782 1101 30-31 0.5321745132453017 1101 32-33 0.42905735581828 1101 34-35 0.49314503583185143 1101 36-37 0.3926765943336292 1101 38-39 0.532898987143902 1101 40-41 0.462286684078947 1101 42-43 0.3223214360179085 1101 44-45 0.4455723199646542 1101 46-47 0.5287974666497988 1101 48-49 0.2998424900423302 1101 50-51 0.2545511558116189 1101 52-53 0.47895922514825173 1101 54-55 0.47516730034632104 1101 56-57 0.48885971237542236 1101 58-59 0.4298882265639392 1101 60-61 0.5434442543365989 1101 62-63 0.4968087279580047 1101 64-65 0.4577620524039929 1101 66-67 0.4336940361741384 1101 68-69 0.35493813407773445 1101 70-71 0.2691310947529999 1101 72-73 0.3794965150221188 1101 74-75 0.3140725812384062 1101 76-77 0.211470991462118 1101 78-79 0.1844729854924374 1101 80-81 0.2201296605657852 1101 82-83 0.03531972544111284 1101 84-85 -0.04625575495763101 1101 86-87 -0.017833977063990147 1101 88-89 -0.06611606305286344 1101 90-91 -0.023814683205440446 1101 92-93 6.92676118152491E-4 1101 94-95 0.1648917872744775 1101 96-97 0.09910772553995528 1101 98-99 0.13466359524404936 1101 100-101 -0.22294624672410635 1102 1 0.23685229522991236 1102 2 0.3472846489494792 1102 3 0.2833069167716289 1102 4 0.17090492403941937 1102 5 0.1818628293567741 1102 6 0.24240763562681877 1102 7 0.23522617787416777 1102 8 0.22258306403818295 1102 9 0.24622053831479462 1102 10-11 0.33501003687214137 1102 12-13 0.3510436036401785 1102 14-15 0.4230952377549215 1102 16-17 0.45090809308193514 1102 18-19 0.4531636628616056 1102 20-21 0.4094855834791389 1102 22-23 0.37095710811352944 1102 24-25 0.4437194651381233 1102 26-27 0.48902265319189553 1102 28-29 0.5085818906424819 1102 30-31 0.41957346906530546 1102 32-33 0.3601435211149706 1102 34-35 0.29324182013916555 1102 36-37 0.2644169397581848 1102 38-39 0.42692494169617845 1102 40-41 0.3525012453461329 1102 42-43 0.3535343751255908 1102 44-45 0.33381575294492905 1102 46-47 0.3058963911171517 1102 48-49 0.3635572353360246 1102 50-51 0.2696733630390824 1102 52-53 0.24880341604507805 1102 54-55 0.3630283671547616 1102 56-57 0.39135361223773657 1102 58-59 0.29499066798216944 1102 60-61 0.3108497533210439 1102 62-63 0.3370798603343985 1102 64-65 0.41155572461871515 1102 66-67 0.39599041425999104 1102 68-69 0.195560961542661 1102 70-71 0.23678741504089373 1102 72-73 0.27819401635120045 1102 74-75 0.14560109171428337 1102 76-77 0.1805579473911756 1102 78-79 0.05277680642783622 1102 80-81 0.11491966588210545 1102 82-83 -0.02401655231621902 1102 84-85 0.031526793877549864 1102 86-87 -0.06700449465555991 1102 88-89 -0.14108028012234897 1102 90-91 -0.21745759000624076 1102 92-93 0.04202979903422843 1102 94-95 -0.02815950840839676 1102 96-97 -0.06561643918421112 1102 98-99 -0.21850711318981908 1102 100-101 -0.44291107721525336 1103 1 0.16801127519328318 1103 2 0.21476900918178288 1103 3 0.18010744626521813 1103 4 0.27093014382160163 1103 5 0.2243199366903994 1103 6 0.1764524226704296 1103 7 0.26608111935239975 1103 8 0.28958605538456794 1103 9 0.33340940676590947 1103 10-11 0.3265221181529441 1103 12-13 0.2169077147022449 1103 14-15 0.398134658893909 1103 16-17 0.3860294419625987 1103 18-19 0.3956380402634636 1103 20-21 0.5211275131336137 1103 22-23 0.5527213019318182 1103 24-25 0.40183612307838246 1103 26-27 0.426712962795186 1103 28-29 0.43660256111709117 1103 30-31 0.4680249957606577 1103 32-33 0.41692176399670444 1103 34-35 0.3863987611988975 1103 36-37 0.4419367934834568 1103 38-39 0.5227234874163216 1103 40-41 0.4881252689552653 1103 42-43 0.3476583638595301 1103 44-45 0.43458576345621935 1103 46-47 0.3827093599332514 1103 48-49 0.3346425875464263 1103 50-51 0.3465001144052309 1103 52-53 0.36527373086897796 1103 54-55 0.41003112701522326 1103 56-57 0.42253408079571386 1103 58-59 0.4235364003107307 1103 60-61 0.41405166052138753 1103 62-63 0.3623076379141992 1103 64-65 0.3992051129933074 1103 66-67 0.19549497346662292 1103 68-69 0.23014244565374753 1103 70-71 0.2786061085648015 1103 72-73 0.35872195350399494 1103 74-75 0.2765726289970729 1103 76-77 0.29631712832087587 1103 78-79 0.13413558006415016 1103 80-81 -0.03550658803297324 1103 82-83 0.02912355423431734 1103 84-85 0.023238912500683995 1103 86-87 0.11781691243162484 1103 88-89 0.23147575393898023 1103 90-91 0.2094104480926866 1103 92-93 0.14975733218316023 1103 94-95 0.4152204815219669 1103 96-97 0.23461014289262394 1103 98-99 -0.015305495346883191 1103 100-101 -0.07380418341216455 1104 1 0.1546296593951091 1104 2 0.20912386592177867 1104 3 0.1670176715850431 1104 4 0.230465135690892 1104 5 0.2110866262611779 1104 6 0.14259110235020955 1104 7 0.12550864572317977 1104 8 0.22652124668358908 1104 9 0.23011121460090322 1104 10-11 0.2526170812852726 1104 12-13 0.20580664794695025 1104 14-15 0.3052372191364441 1104 16-17 0.28419962200083404 1104 18-19 0.18293520099815908 1104 20-21 0.20043913774068045 1104 22-23 0.25349793019807265 1104 24-25 0.2758700142088273 1104 26-27 0.201203395981004 1104 28-29 0.09262353552063018 1104 30-31 0.3039412770072616 1104 32-33 0.3598358228985248 1104 34-35 0.422113898631018 1104 36-37 0.3315391711389779 1104 38-39 0.42379389445866167 1104 40-41 0.2739807375480581 1104 42-43 0.29563097729823795 1104 44-45 0.4504945325514669 1104 46-47 0.3331456311040384 1104 48-49 0.2847860487701581 1104 50-51 0.2951129002656998 1104 52-53 0.3455469555642807 1104 54-55 0.3711763872799665 1104 56-57 0.2731966108107784 1104 58-59 0.382583176867449 1104 60-61 0.3661148937572918 1104 62-63 0.17506234679597554 1104 64-65 0.276233505646303 1104 66-67 0.22648267231878805 1104 68-69 0.24778161794582587 1104 70-71 0.1999540329603633 1104 72-73 0.24555392724723646 1104 74-75 0.09297140201235266 1104 76-77 0.06153176866918031 1104 78-79 -0.06451677873647199 1104 80-81 -0.04709400696806654 1104 82-83 -0.08844675667037905 1104 84-85 -0.015993894137437792 1104 86-87 -0.15671100698564544 1104 88-89 -0.14846769625500755 1104 90-91 -0.2773379082515781 1104 92-93 -0.30468907144890167 1104 94-95 -0.26771963142859434 1104 96-97 -0.3437313112154321 1104 98-99 -0.4582173184893783 1104 100-101 -0.49064019642726464 1105 1 0.3059675253116829 1105 2 0.31378696117669946 1105 3 0.31638230895482167 1105 4 0.229074263993148 1105 5 0.19513683333709508 1105 6 0.1830606628969491 1105 7 0.26743127141755707 1105 8 0.2339576044090066 1105 9 0.2854223938735245 1105 10-11 0.3888440591076616 1105 12-13 0.34361602502301025 1105 14-15 0.4186865526770873 1105 16-17 0.3722210222015505 1105 18-19 0.41752041309196386 1105 20-21 0.3827337933444497 1105 22-23 0.39762700987636634 1105 24-25 0.32429892285512807 1105 26-27 0.41925433807042367 1105 28-29 0.38972517940658946 1105 30-31 0.3719561751654865 1105 32-33 0.3950620515725305 1105 34-35 0.3892474873344227 1105 36-37 0.42834186241916683 1105 38-39 0.5499518940911585 1105 40-41 0.5276536848108435 1105 42-43 0.22402582089584655 1105 44-45 0.2962053047396864 1105 46-47 0.2977391827740661 1105 48-49 0.3197606766695742 1105 50-51 0.26545863930763147 1105 52-53 0.32034164650524133 1105 54-55 0.32951667026016196 1105 56-57 0.22415142161547408 1105 58-59 0.3204999789933396 1105 60-61 0.2845107267961495 1105 62-63 0.24973053060588768 1105 64-65 0.2313107527380538 1105 66-67 0.36487369950015136 1105 68-69 0.21228611990350998 1105 70-71 0.08476186933504692 1105 72-73 0.24741973074420542 1105 74-75 0.28645169092919787 1105 76-77 0.2600553809778283 1105 78-79 0.133512819691731 1105 80-81 0.24279319672961464 1105 82-83 0.27637931369303104 1105 84-85 0.2220232656007859 1105 86-87 0.17007952830207174 1105 88-89 0.03073495544753513 1105 90-91 0.13305829229402732 1105 92-93 0.3026843860828663 1105 94-95 0.3822294830214865 1105 96-97 0.39030970819174726 1105 98-99 0.51563528303436 1105 100-101 0.05277931252717849 1106 1 0.04972667409413489 1106 2 0.1121066853451218 1106 3 0.055640054586994836 1106 4 0.11314035358449814 1106 5 0.06603083281411415 1106 6 0.1296498350517581 1106 7 0.10662297059076309 1106 8 0.07051741958675706 1106 9 0.253345111429212 1106 10-11 0.2611236863360489 1106 12-13 0.3058823351286577 1106 14-15 0.4165308771296452 1106 16-17 0.33707325335877414 1106 18-19 0.33513716031733054 1106 20-21 0.37272398072769164 1106 22-23 0.2812385919990774 1106 24-25 0.3402949708506853 1106 26-27 0.3853664391295908 1106 28-29 0.37495395625312256 1106 30-31 0.32465006195915436 1106 32-33 0.3990375092475418 1106 34-35 0.39307979941877136 1106 36-37 0.41676459210584227 1106 38-39 0.5579776171650934 1106 40-41 0.5960418277192048 1106 42-43 0.5126778261472111 1106 44-45 0.4601702219389878 1106 46-47 0.4528873020674524 1106 48-49 0.5432490888857444 1106 50-51 0.5603258859251099 1106 52-53 0.48505207995872013 1106 54-55 0.37817255742584877 1106 56-57 0.4401821103048178 1106 58-59 0.43209679366697884 1106 60-61 0.4438647924274548 1106 62-63 0.4053001341384288 1106 64-65 0.4360485752536931 1106 66-67 0.3770313699202319 1106 68-69 0.3339804946322502 1106 70-71 0.2641855668045281 1106 72-73 0.2929097011176438 1106 74-75 0.3967370116319344 1106 76-77 0.3923370030788611 1106 78-79 0.3082627192741576 1106 80-81 0.2782260436554864 1106 82-83 0.3518053133533634 1106 84-85 0.39234589581340273 1106 86-87 0.341322091336 1106 88-89 0.35633685435062645 1106 90-91 0.46545650661343885 1106 92-93 0.5025071389531881 1106 94-95 0.4230736915557678 1106 96-97 0.33955974391075117 1106 98-99 0.43053617290930646 1106 100-101 0.31099266146005533 1107 1 0.07051588136874187 1107 2 0.06870459314169963 1107 3 0.04269302021713628 1107 4 0.11520814557036374 1107 5 0.12983262633647286 1107 6 0.11965909703669553 1107 7 0.12927354964431714 1107 8 0.13455255769084573 1107 9 0.24549216877453972 1107 10-11 0.23077722348092067 1107 12-13 0.2290859408409318 1107 14-15 0.2988686667461735 1107 16-17 0.2880242222772722 1107 18-19 0.28205575536755845 1107 20-21 0.3641342076822198 1107 22-23 0.3345919331344973 1107 24-25 0.20320502907912896 1107 26-27 0.3198343057857258 1107 28-29 0.30932130524328727 1107 30-31 0.33764645768284396 1107 32-33 0.2052223971344418 1107 34-35 0.3796615884896326 1107 36-37 0.4075708155853306 1107 38-39 0.585924220744964 1107 40-41 0.5292334790090649 1107 42-43 0.49340715361223175 1107 44-45 0.6057189047985716 1107 46-47 0.39919830372599563 1107 48-49 0.43613674603435015 1107 50-51 0.4527540337435241 1107 52-53 0.4431963443488911 1107 54-55 0.4586540011467335 1107 56-57 0.4919469133260961 1107 58-59 0.455916788066709 1107 60-61 0.36142953657410004 1107 62-63 0.4342842236278699 1107 64-65 0.488882275955163 1107 66-67 0.33201561018617554 1107 68-69 0.353158726813696 1107 70-71 0.28310083759193816 1107 72-73 0.31608530548011515 1107 74-75 0.2888959157021844 1107 76-77 0.11762940178936176 1107 78-79 0.16518928393932342 1107 80-81 0.1910879970009347 1107 82-83 0.2403760957397978 1107 84-85 0.09916817340011619 1107 86-87 0.07298120721133117 1107 88-89 0.06713765705598718 1107 90-91 0.25416388532810785 1107 92-93 0.3075118417439917 1107 94-95 0.17890281870343472 1107 96-97 0.057146386548911465 1107 98-99 0.19756045510547438 1107 100-101 0.11962676997605826 1108 1 0.13454272740309747 1108 2 0.12656284202077828 1108 3 0.10499863501507178 1108 4 0.21000949762675702 1108 5 0.3342487576047688 1108 6 0.1877496499343536 1108 7 0.21724798748218888 1108 8 0.2617814135813603 1108 9 0.34634646602692953 1108 10-11 0.2492854166647689 1108 12-13 0.16108031022860558 1108 14-15 0.16194259729194016 1108 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-1.1869102436338501 2316 80-81 -1.1214017616496967 2316 82-83 -0.9162998373948916 2316 84-85 -0.9306261028574809 2316 86-87 -0.8330996067160896 2316 88-89 -1.0384469864227697 2316 90-91 -0.9155163196211653 2316 92-93 -0.8997367917237185 2316 94-95 -1.1493958129300026 2316 96-97 -1.0686636481392036 2316 98-99 -0.9885374990703504 2316 100-101 -0.6049979176882623 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8797.0 3 2519.0 4 2472.0 5 2839.0 6 3030.0 7 3672.0 8 4563.0 9 5720.0 10 7089.0 11 8467.0 12 10536.0 13 14493.0 14 13255.0 15 13178.0 16 15192.0 17 18362.0 18 16773.0 19 13875.0 20 14631.0 21 15831.0 22 17325.0 23 19604.0 24 22504.0 25 25954.0 26 29867.0 27 35275.0 28 41438.0 29 49934.0 30 59786.0 31 73438.0 32 92197.0 33 119153.0 34 161835.0 35 233227.0 36 371634.0 37 611692.0 38 618049.0 39 103589.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.037328895598385 18.641688399457394 14.39706034860162 23.9239223563426 2 15.030531630407824 19.675931876495056 41.00291316705655 24.290623326040574 3 19.261100601563054 23.683657392207646 29.37851223016084 27.676729776068456 4 13.026129457690763 16.159906388667693 36.92860051336143 33.88536364028011 5 14.337747711593954 36.82633825499158 34.15953955654695 14.676374476867512 6 32.82160821380446 35.29795734701977 17.614049658226875 14.266384780948895 7 29.255639606855205 31.896536457564707 20.592807623994958 18.25501631158513 8 25.87073660363154 35.84518895433128 19.832851630906788 18.4512228111304 9 26.194960625765244 17.455193100081186 20.38376249478263 35.96608377937095 10-11 25.111426319389707 26.37199515265996 27.450858180580777 21.065720347369556 12-13 25.71257677366148 23.474167154979707 27.90547995746251 22.907776113896304 14-15 22.664850189223152 25.223885416778792 25.81895134365973 26.292313050338322 16-17 22.75538997907743 28.42250498237007 26.40632701535763 22.415778023194864 18-19 22.604775785730492 27.23306640682988 28.271869513967584 21.890288293472036 20-21 23.1710099410938 26.55539901717666 28.133448254188075 22.140142787541457 22-23 22.82979600873442 26.376786931361867 28.047388139073508 22.746028920830206 24-25 22.803298488554535 26.55647971353129 28.126743649923284 22.513478147990888 26-27 22.62077471282652 26.80902881129821 27.951922472451386 22.61827400342389 28-29 22.47578446524389 26.936027228367514 28.14768958271832 22.440498723670274 30-31 22.68699132145853 26.628149007877695 28.07895998501107 22.6058996856527 32-33 22.477883235847848 26.788948485055265 27.964747220982204 22.768421058114683 34-35 23.012495756132044 26.78223322072009 27.70713554218544 22.498135480962425 36-37 22.977122248559727 26.566069832286942 27.729697469353265 22.727110449800065 38-39 22.668748206769568 26.793683093353838 27.902439211229314 22.635129488647284 40-41 22.916473013343193 26.604972634391927 27.900926785640305 22.577627566624574 42-43 22.72080518636632 26.621026763747945 27.731211975695093 22.92695607419064 44-45 22.824302992467675 26.800839691532648 27.588638217806093 22.786219098193584 46-47 22.82561581364618 26.9625955260636 27.287727646905473 22.92406101338475 48-49 22.934847636455785 26.87140295679238 27.34535707661239 22.848392330139443 50-51 22.81549483835699 27.022829932148127 27.33456436506227 22.82711086443262 52-53 22.979910607383903 26.93779350183775 27.293465701047616 22.78883018973073 54-55 22.74223130872652 26.77324251627291 27.41355433482499 23.07097184017558 56-57 22.768920786360177 27.177982527882723 27.27555856842979 22.77753811732731 58-59 22.843477561597645 26.78547619486916 27.36957333772432 23.001472905808875 60-61 22.8938819702924 26.853601871961857 27.516388278629183 22.736127879116555 62-63 22.829479317401198 26.836650675566386 27.514036538964792 22.819833468067625 64-65 22.6105028569677 26.74424113058175 27.491330172393376 23.153925840057184 66-67 22.524954323703426 26.96961938451517 27.368296382994657 23.137129908786743 68-69 22.591204486593938 26.791986393619325 27.580094606430144 23.03671451335659 70-71 22.592484077054444 26.74110729358351 27.494438030285824 23.17197059907622 72-73 22.67914564142988 26.687045028912117 27.39557947099741 23.238229858660596 74-75 22.6184017404411 26.84885726512414 27.300652978916958 23.232088015517807 76-77 22.74732670956934 26.710335562054905 27.297192577042395 23.245145151333357 78-79 22.715625988581937 26.811534780586822 27.287638453702463 23.185200777128777 80-81 22.617485080147365 27.043100132889798 27.30981986494441 23.02959492201843 82-83 22.836041410376623 26.90911813588879 27.216970906346067 23.037869547388517 84-85 22.716725551174843 26.767697611376875 27.173907554369563 23.34166928307872 86-87 22.70908127832382 26.661574052953203 27.430585423052023 23.198759245670956 88-89 22.75765215802725 26.69771004304543 27.269385115793547 23.275252683133772 90-91 22.622218978351402 26.87658265718659 27.37320844120994 23.127989923252066 92-93 22.78225005765502 26.57817985718277 27.396236642673355 23.243333442488858 94-95 22.645045047555808 26.563078074301465 27.363193872055326 23.428683006087393 96-97 22.73387222501413 26.476298143375615 27.38401214465941 23.40581748695085 98-99 22.61197800595781 26.516601516745208 27.210790868316693 23.66062960898029 100-101 22.823008149930015 26.09879173743859 27.614927386878406 23.463272725752994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1792.0 1 978.0 2 167.5 3 171.0 4 163.0 5 176.5 6 252.5 7 505.5 8 863.5 9 1108.0 10 1243.5 11 1374.5 12 1589.0 13 1833.5 14 2023.5 15 2170.5 16 2394.5 17 2596.0 18 2706.5 19 2890.0 20 3138.5 21 3471.0 22 4098.0 23 4873.0 24 5984.0 25 7812.0 26 10535.0 27 14201.5 28 17674.0 29 21983.5 30 27466.0 31 33303.0 32 39419.0 33 45163.5 34 51276.5 35 57687.0 36 65008.0 37 72957.0 38 78810.5 39 82430.0 40 86022.0 41 92634.5 42 98694.0 43 103006.0 44 108290.0 45 113150.5 46 114481.5 47 115416.5 48 122978.5 49 124917.5 50 119080.5 51 113394.0 52 107000.5 53 104182.0 54 101711.0 55 93405.0 56 82813.0 57 73842.5 58 65355.0 59 55887.5 60 46085.5 61 37548.0 62 30851.0 63 23965.0 64 17779.5 65 13755.0 66 10448.0 67 8348.5 68 6359.0 69 4266.5 70 3123.0 71 2345.5 72 1774.5 73 1456.0 74 1183.5 75 924.0 76 667.5 77 437.5 78 264.5 79 172.5 80 104.5 81 68.5 82 56.0 83 42.5 84 29.0 85 16.5 86 13.0 87 16.0 88 15.5 89 10.5 90 8.5 91 5.5 92 2.5 93 2.5 94 4.5 95 4.5 96 4.5 97 3.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28742243185818966 2 0.10989579158455713 3 0.12096518139051662 4 0.12273489574820921 5 0.1292932489561288 6 0.058782276900612496 7 0.06822075347497294 8 0.07300939232519994 9 0.0700598683957123 10-11 0.06561823236071915 12-13 0.06969551543971676 14-15 0.06551413151614899 16-17 0.06650308953956544 18-19 0.07163873120502627 20-21 0.07488320752746266 22-23 0.08357562804907036 24-25 0.09103618857659791 26-27 0.09153934265868699 28-29 0.08574439564428186 30-31 0.0819794150989947 32-33 0.0750046585127945 34-35 0.24585149459317562 36-37 0.37325357820627997 38-39 0.34384508961521204 40-41 0.32687665195027726 42-43 0.351791454084067 44-45 0.36534191401894844 46-47 0.3706163568105028 48-49 0.3807835392968543 50-51 0.37432928693350487 52-53 0.3740169843997944 54-55 0.361143179954619 56-57 0.33666213133987155 58-59 0.3216022091587229 60-61 0.27914641471486257 62-63 0.35114949887588437 64-65 0.37979458127343785 66-67 0.3843403181530012 68-69 0.3956873102111478 70-71 0.396797719219896 72-73 0.38050593704466723 74-75 0.3835942621002484 76-77 0.3675974323179684 78-79 0.34587505608433 80-81 0.3432725349700762 82-83 0.3580548548990378 84-85 0.3651337123298081 86-87 0.3588703115148373 88-89 0.37422518608893474 90-91 0.3973355735835085 92-93 0.39207848093271586 94-95 0.39207848093271586 96-97 0.3963986659823771 98-99 0.38637028462211914 100-101 0.36463055824771906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2881821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.558351015329066 #Duplication Level Percentage of deduplicated Percentage of total 1 73.55151919451629 39.39298082730616 2 14.273149244530462 15.288926746654823 3 5.178745485650921 8.320952056186282 4 2.373538858904441 5.084913094149105 5 1.328616516755542 3.557925488457857 6 0.7743082994857946 2.4882405418765634 7 0.4842576128915572 1.8155227449163953 8 0.3617415537378226 1.5499424889536657 9 0.2569345980518905 1.2384894051401072 >10 1.2812385260051917 12.181605406314803 >50 0.08591601066033934 3.1803895745329407 >100 0.04717194329660685 4.508217272505584 >500 0.002276714612779108 0.8237023976132518 >1k 5.854409003536832E-4 0.568191955392318 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4306 0.1494194122396915 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3396 0.11784215605341207 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.7350140761692E-5 0.0 22-23 0.0 3.4700281523384E-5 0.0 3.4700281523384E-5 0.0 24-25 0.0 5.2050422285076E-5 0.0 1.04100844570152E-4 0.0 26-27 0.0 6.9400563046768E-5 0.0 2.7760225218707196E-4 0.0 28-29 0.0 6.9400563046768E-5 0.0 0.00156151266855228 0.0 30-31 0.0 6.9400563046768E-5 0.0 0.006315451237255887 0.0 32-33 0.0 6.9400563046768E-5 0.0 0.017211339635598465 0.0 34-35 0.0 6.9400563046768E-5 0.0 0.033121418714070025 0.0 36-37 0.0 6.9400563046768E-5 0.0 0.05918133013813141 0.0 38-39 0.0 1.04100844570152E-4 0.0 0.09854879952641056 0.0 40-41 0.0 1.04100844570152E-4 0.0 0.143364213113861 0.0 42-43 0.0 1.04100844570152E-4 0.0 0.18920328500625125 0.0 44-45 0.0 1.04100844570152E-4 0.0 0.2474477075432513 0.0 46-47 0.0 1.04100844570152E-4 0.0 0.3201794976162642 0.0 48-49 0.0 1.21450985331844E-4 0.0 0.3980989797770229 1.7350140761692E-5 50-51 0.0 1.38801126093536E-4 0.0 0.4823512633157993 3.4700281523384E-5 52-53 0.0 1.38801126093536E-4 0.0 0.567089350795903 3.4700281523384E-5 54-55 0.0 1.38801126093536E-4 0.0 0.6672517134131509 3.4700281523384E-5 56-57 0.0 1.38801126093536E-4 0.0 0.7836538077833426 3.4700281523384E-5 58-59 0.0 1.38801126093536E-4 0.0 0.9119927989975782 3.4700281523384E-5 60-61 0.0 1.38801126093536E-4 0.0 1.0558081157712431 3.4700281523384E-5 62-63 0.0 1.38801126093536E-4 0.0 1.204533522380467 3.4700281523384E-5 64-65 0.0 1.38801126093536E-4 0.0 1.3686658539860734 3.4700281523384E-5 66-67 0.0 1.38801126093536E-4 0.0 1.5562208756199638 3.4700281523384E-5 68-69 0.0 1.38801126093536E-4 0.0 1.7691765033289715 3.4700281523384E-5 70-71 0.0 2.08201689140304E-4 0.0 2.006075325289114 3.4700281523384E-5 72-73 0.0 2.08201689140304E-4 0.0 2.25090316157735 5.2050422285076E-5 74-75 0.0 2.08201689140304E-4 0.0 2.5063145837302176 6.9400563046768E-5 76-77 0.0 2.08201689140304E-4 0.0 2.7874215643511517 6.9400563046768E-5 78-79 0.0 2.08201689140304E-4 3.4700281523384E-5 3.1086767706946405 6.9400563046768E-5 80-81 0.0 2.08201689140304E-4 3.4700281523384E-5 3.4630013453299147 6.9400563046768E-5 82-83 0.0 2.25551829901996E-4 3.4700281523384E-5 3.8237454720470145 6.9400563046768E-5 84-85 0.0 2.42901970663688E-4 3.4700281523384E-5 4.202516395015513 6.9400563046768E-5 86-87 0.0 2.42901970663688E-4 3.4700281523384E-5 4.608839341513578 6.9400563046768E-5 88-89 0.0 2.42901970663688E-4 3.4700281523384E-5 5.0515802334704345 6.9400563046768E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8975 0.0 31.807991 1 GGTATCA 6065 0.0 30.622608 1 ATCAACG 10550 0.0 26.56571 3 TCAACGC 10700 0.0 26.193293 4 CAACGCA 10840 0.0 25.811255 5 TATCAAC 11155 0.0 25.252436 2 AACGCAG 11200 0.0 25.050188 6 GTACATG 9305 0.0 24.401007 1 TACATGG 9405 0.0 23.244944 2 ACGCAGA 12535 0.0 22.231068 7 ACATGGG 9490 0.0 22.037321 3 CGCAGAG 12740 0.0 21.798946 8 GCAGAGT 13755 0.0 19.880281 9 GAGTACT 5975 0.0 19.671782 12-13 CATGGGG 5600 0.0 18.884403 4 GTACTTT 6335 0.0 18.516483 14-15 AGTACTT 6295 0.0 17.768312 12-13 GTGTAGC 1620 0.0 17.5927 1 AGAGTAC 11895 0.0 17.530853 10-11 ATTCGCG 330 0.0 17.28336 80-81 >>END_MODULE