FastQCFastQC Report
Fri 27 May 2016
SRR939256_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939256_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2881821
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44710.15514495869104986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35670.12377590419391073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.030.1638681
GTATCAA88100.029.5385671
ATCAACG104000.024.730183
TCAACGC105600.024.3554824
CAACGCA107600.023.9468785
TATCAAC110450.023.6726682
GTACATG86750.023.5818021
AACGCAG110650.023.2867976
TACATGG88700.022.255182
ACATGGG89600.021.0783393
ACGCAGA124250.020.5851357
CGCAGAG126400.020.009748
GTATAGG8150.019.8472021
GAGTACT59850.019.54436912-13
CATGGGG53000.018.71254
GTACTTT63600.018.16815214-15
GCAGAGT139000.017.7521089
ATTCGCG3650.016.92050280-81
AGTACTT64550.016.9083212-13
AGGCGTA1700.001800391216.7480285
Produced by FastQC (version 0.11.5)