##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939256_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2881821 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.339162633626444 34.0 31.0 34.0 31.0 34.0 2 32.54281927989282 34.0 31.0 34.0 31.0 34.0 3 32.6378709850473 34.0 31.0 34.0 31.0 34.0 4 36.149870515899494 37.0 35.0 37.0 35.0 37.0 5 36.08389140061093 37.0 35.0 37.0 35.0 37.0 6 36.11405080329417 37.0 36.0 37.0 35.0 37.0 7 36.06074561882921 37.0 35.0 37.0 35.0 37.0 8 36.039120056381016 37.0 35.0 37.0 35.0 37.0 9 37.766242941528986 39.0 38.0 39.0 35.0 39.0 10-11 37.68581098548452 39.0 37.0 39.0 35.0 39.0 12-13 37.6078991373857 39.0 37.0 39.0 35.0 39.0 14-15 38.950759780014096 40.0 38.0 41.0 35.0 41.0 16-17 38.8908252802655 40.0 38.0 41.0 35.0 41.0 18-19 38.982471673292686 40.0 38.5 41.0 35.0 41.0 20-21 38.980858977708884 40.0 38.0 41.0 35.0 41.0 22-23 38.92924040042737 40.0 38.0 41.0 35.0 41.0 24-25 38.86823921402474 40.0 38.0 41.0 35.0 41.0 26-27 38.719256504828024 40.0 38.0 41.0 34.0 41.0 28-29 38.582317916345254 40.0 38.0 41.0 34.0 41.0 30-31 38.42854656829831 40.0 38.0 41.0 34.0 41.0 32-33 38.26949331689928 40.0 38.0 41.0 34.0 41.0 34-35 38.258809794223865 40.0 38.0 41.0 34.0 41.0 36-37 38.201264582359556 40.0 38.0 41.0 33.5 41.0 38-39 38.116976210527994 40.0 38.0 41.0 33.0 41.0 40-41 37.96836288582809 40.0 38.0 41.0 33.0 41.0 42-43 37.83194879210055 40.0 37.0 41.0 33.0 41.0 44-45 37.662125961327924 40.0 37.0 41.0 33.0 41.0 46-47 37.45006317186251 40.0 37.0 41.0 32.0 41.0 48-49 37.2139624563774 40.0 37.0 41.0 31.5 41.0 50-51 37.00823784683365 40.0 36.0 41.0 31.0 41.0 52-53 36.79045055192533 39.0 36.0 41.0 31.0 41.0 54-55 36.52576513253252 39.0 35.5 41.0 30.5 41.0 56-57 36.29367871911545 39.0 35.0 40.0 30.0 41.0 58-59 36.006514110348974 38.5 35.0 40.0 29.5 41.0 60-61 35.6915254278458 38.0 35.0 40.0 29.0 41.0 62-63 35.31588603178338 38.0 34.0 40.0 28.0 41.0 64-65 34.89532469226923 37.0 34.0 40.0 28.0 41.0 66-67 34.532706229845644 37.0 34.0 39.0 27.5 41.0 68-69 34.1494742386845 36.0 34.0 39.0 26.5 40.0 70-71 33.75563176894054 36.0 33.0 39.0 26.0 40.0 72-73 33.259145345946195 35.0 33.0 38.0 26.0 40.0 74-75 32.75489889899477 35.0 32.0 37.0 25.5 39.0 76-77 30.732864914233048 33.5 29.5 35.5 21.5 37.5 78-79 31.7215109821186 34.5 31.0 36.0 23.5 38.5 80-81 31.764470104145957 35.0 32.0 36.0 24.0 37.5 82-83 31.456715215830545 34.5 31.5 35.5 23.5 37.0 84-85 31.08470182568591 34.0 31.0 35.0 22.0 36.5 86-87 30.56050011433743 34.0 31.0 35.0 19.5 36.0 88-89 30.072178320582715 34.0 30.0 35.0 17.5 36.0 90-91 29.663848309801338 34.0 30.0 35.0 11.0 35.0 92-93 29.32122536410138 34.0 30.0 35.0 6.5 35.0 94-95 28.943477058429373 34.0 29.0 35.0 2.0 35.0 96-97 28.410577548015645 34.0 29.0 35.0 2.0 35.0 98-99 27.675161469084998 33.5 29.0 35.0 2.0 35.0 100-101 24.272074844343212 30.0 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22347417692462557 1101 2 0.19680913148434342 1101 3 0.2257442465080146 1101 4 0.1914179393891473 1101 5 0.2214180277516249 1101 6 0.14679136622995514 1101 7 0.2164219521440458 1101 8 0.20753441286413477 1101 9 0.21468189379482538 1101 10-11 0.2704494820177814 1101 12-13 0.33999521593804616 1101 14-15 0.39038019618521247 1101 16-17 0.31983839632341216 1101 18-19 0.32560911685297356 1101 20-21 0.32319700215261093 1101 22-23 0.30447137519517753 1101 24-25 0.2860257767721137 1101 26-27 0.3305641753416637 1101 28-29 0.3501361842685995 1101 30-31 0.37919948084588384 1101 32-33 0.4094019736467942 1101 34-35 0.3765881365739361 1101 36-37 0.48339524973828674 1101 38-39 0.2784834845196542 1101 40-41 0.3963395386059787 1101 42-43 0.38582161617156174 1101 44-45 0.41387467210868323 1101 46-47 0.43937938179126945 1101 48-49 0.38312122236656876 1101 50-51 0.5054224477643103 1101 52-53 0.4876048248236273 1101 54-55 0.3612519840137125 1101 56-57 0.38585564683351237 1101 58-59 0.2606460150295149 1101 60-61 0.28403019062737656 1101 62-63 0.20920963531553127 1101 64-65 0.24922425663805114 1101 66-67 0.2683790463730702 1101 68-69 0.3312615613126013 1101 70-71 0.20409209556205354 1101 72-73 0.1361991049272646 1101 74-75 0.0055668755571574025 1101 76-77 0.15423108063874835 1101 78-79 0.29961227660232836 1101 80-81 0.4461610309083959 1101 82-83 0.3559396948461213 1101 84-85 0.23014952729644733 1101 86-87 0.408521590384364 1101 88-89 0.48260325510862145 1101 90-91 0.4506336790623138 1101 92-93 0.35500902442593585 1101 94-95 0.4260910125103017 1101 96-97 0.2843413960965009 1101 98-99 0.44128177438720684 1101 100-101 0.31020786612586804 1102 1 0.15193694222949716 1102 2 0.13397430549161982 1102 3 0.16635412395999793 1102 4 0.06277114758445634 1102 5 0.12399439874904772 1102 6 0.1567274340607554 1102 7 0.16606548599810367 1102 8 0.19771557672951445 1102 9 0.3289214554028064 1102 10-11 0.26358733831353476 1102 12-13 0.2399066105862815 1102 14-15 0.2529464798041303 1102 16-17 0.3174767229090989 1102 18-19 0.2805955534183511 1102 20-21 0.32812345971063905 1102 22-23 0.22959440031820577 1102 24-25 0.2992225215478115 1102 26-27 0.3342058552991318 1102 28-29 0.35150138826538324 1102 30-31 0.35458150622791607 1102 32-33 0.3391945008077428 1102 34-35 0.39061300059879756 1102 36-37 0.4455805303446141 1102 38-39 0.4101101424621021 1102 40-41 0.4952496927792893 1102 42-43 0.28260524190255154 1102 44-45 0.49549042477706706 1102 46-47 0.5251418649221691 1102 48-49 0.44037800067598454 1102 50-51 0.33044459910224333 1102 52-53 0.48517976450383316 1102 54-55 0.4998757384269439 1102 56-57 0.4743117326580304 1102 58-59 0.5655983726134508 1102 60-61 0.3697190631583567 1102 62-63 0.35414004603541827 1102 64-65 0.35469325158073417 1102 66-67 0.3765689071945104 1102 68-69 0.4436915290057257 1102 70-71 0.37935688924790156 1102 72-73 0.514255648773279 1102 74-75 0.4742128494662907 1102 76-77 0.5754445650101268 1102 78-79 0.5045428236381362 1102 80-81 0.5876978566557476 1102 82-83 0.5025317967013834 1102 84-85 0.6129600890543756 1102 86-87 0.6154116788534019 1102 88-89 0.685885061022006 1102 90-91 0.8116323295346497 1102 92-93 0.8110048804217946 1102 94-95 0.745460823985379 1102 96-97 0.5456106336815303 1102 98-99 0.2641883025568923 1102 100-101 0.1502765693524637 1103 1 0.1058695682514994 1103 2 0.11546045730313637 1103 3 0.12039046176740698 1103 4 0.12473018249527712 1103 5 0.15568162349782 1103 6 0.07574924155767349 1103 7 0.12903337311338703 1103 8 0.11366410400692928 1103 9 0.12331306927697483 1103 10-11 0.20536756253201816 1103 12-13 0.18845520907608915 1103 14-15 0.28691582071193267 1103 16-17 0.22859336226957083 1103 18-19 0.2195552282659179 1103 20-21 0.2761117708898553 1103 22-23 0.2550688170117468 1103 24-25 0.34262415236613464 1103 26-27 0.30566188957366336 1103 28-29 0.29788231906418616 1103 30-31 0.32332306346417283 1103 32-33 0.35105868740732404 1103 34-35 0.46642654417008345 1103 36-37 0.3774973982669749 1103 38-39 0.37462015224847534 1103 40-41 0.45662847471864154 1103 42-43 0.4784870311790925 1103 44-45 0.6016178114807005 1103 46-47 0.42030029114685163 1103 48-49 0.46906550290326976 1103 50-51 0.6056228813365863 1103 52-53 0.4726167797730696 1103 54-55 0.49148650581191333 1103 56-57 0.5092667924072813 1103 58-59 0.3613793589079606 1103 60-61 0.2322472640832558 1103 62-63 0.22888765784692566 1103 64-65 0.2513288473539248 1103 66-67 0.2761002091170326 1103 68-69 0.2750732989410025 1103 70-71 0.32180880337758566 1103 72-73 0.2871357478979846 1103 74-75 0.3702114020700904 1103 76-77 0.47250064277965365 1103 78-79 0.57177669173441 1103 80-81 0.5668578795241253 1103 82-83 0.6667723538270813 1103 84-85 0.4756976172172287 1103 86-87 0.5119890224123189 1103 88-89 0.5424506718996547 1103 90-91 0.9051636076994036 1103 92-93 0.6881129633315233 1103 94-95 0.7135682316263008 1103 96-97 0.41864174691256295 1103 98-99 0.22630030092918219 1103 100-101 0.09962744931878831 1104 1 0.09187744110535334 1104 2 0.07716845819147977 1104 3 0.12200274234626107 1104 4 0.05542745621035294 1104 5 0.03853051746767733 1104 6 0.027965996934298687 1104 7 0.08908184437567712 1104 8 0.0780967502665817 1104 9 0.08136634891233996 1104 10-11 0.12536687689915738 1104 12-13 0.10090250104985898 1104 14-15 0.09931407503103884 1104 16-17 0.06527009978993448 1104 18-19 0.04835016889160215 1104 20-21 0.045492972327309644 1104 22-23 0.05461220043106607 1104 24-25 0.03429140281441079 1104 26-27 0.11192410240901296 1104 28-29 0.13652876055510887 1104 30-31 0.12950225510303426 1104 32-33 0.14785096896991945 1104 34-35 0.10962573691190869 1104 36-37 0.20402132908959913 1104 38-39 0.2736654084888528 1104 40-41 0.3603724027689168 1104 42-43 0.3980889765878075 1104 44-45 0.31191571895900694 1104 46-47 0.3857427553709698 1104 48-49 0.29639251847756753 1104 50-51 0.24978054410359363 1104 52-53 0.208021519284749 1104 54-55 0.23764152143987616 1104 56-57 0.2723190063306973 1104 58-59 0.3966020330847897 1104 60-61 0.3239374683273297 1104 62-63 0.37717782363593244 1104 64-65 0.343521575770275 1104 66-67 0.3252969007894251 1104 68-69 0.31785462826388766 1104 70-71 0.20217205464862076 1104 72-73 0.2783892338194107 1104 74-75 0.33825204221270155 1104 76-77 0.3227029044737897 1104 78-79 0.33642806978194173 1104 80-81 0.3414937861170735 1104 82-83 0.3371532377867048 1104 84-85 0.23691062098969695 1104 86-87 0.30082326768703993 1104 88-89 0.5879509081448901 1104 90-91 0.5718074944626643 1104 92-93 0.6695492300307784 1104 94-95 0.6616957086613269 1104 96-97 0.6686023230230269 1104 98-99 0.8465412263546845 1104 100-101 0.6513103053671863 1105 1 0.05382918464218278 1105 2 0.1244792982271008 1105 3 0.12860372896052752 1105 4 0.1235490726746491 1105 5 0.1628863205406006 1105 6 0.13018375881819821 1105 7 0.19733095741227658 1105 8 0.17014446960450158 1105 9 0.13502798827514084 1105 10-11 0.1478392078813897 1105 12-13 0.16459212746510588 1105 14-15 0.24495275935687744 1105 16-17 0.2135654338677 1105 18-19 0.2414862736027601 1105 20-21 0.21291561922399183 1105 22-23 0.25539385901728906 1105 24-25 0.23703992925369022 1105 26-27 0.19944936849802986 1105 28-29 0.21245565498173136 1105 30-31 0.3188594009890764 1105 32-33 0.21821013614308526 1105 34-35 0.31861622414105284 1105 36-37 0.25366018303393645 1105 38-39 0.23320786258585002 1105 40-41 0.2990178576998659 1105 42-43 0.2476196987385677 1105 44-45 0.36339422688160994 1105 46-47 0.3406631053504796 1105 48-49 0.3388572166273107 1105 50-51 0.37365364119605005 1105 52-53 0.3453799938490434 1105 54-55 0.22011993041834188 1105 56-57 0.2759318803748272 1105 58-59 0.2433771613587794 1105 60-61 0.12580609879928772 1105 62-63 -0.01615758281239721 1105 64-65 0.04433574456894718 1105 66-67 0.18420257876869783 1105 68-69 0.3177069188203774 1105 70-71 0.17819153250633946 1105 72-73 0.10834668784573154 1105 74-75 0.2718965783767757 1105 76-77 0.2619526253100055 1105 78-79 0.3099965603186341 1105 80-81 0.38953045198276826 1105 82-83 0.4354733078956805 1105 84-85 0.43621306490958034 1105 86-87 0.4131637202857412 1105 88-89 0.6313195315494156 1105 90-91 0.6647937694990596 1105 92-93 0.5633595682783401 1105 94-95 0.6953077346129568 1105 96-97 0.6136423953935868 1105 98-99 0.8120655595419564 1105 100-101 0.7833844569213184 1106 1 0.058080150403164055 1106 2 0.04870621768546357 1106 3 0.036196915122587825 1106 4 0.049914010361838734 1106 5 0.05232185245721155 1106 6 0.11017302963833231 1106 7 0.09382159903340437 1106 8 0.05932632920320913 1106 9 0.1168315148864707 1106 10-11 0.10628102364680814 1106 12-13 0.1353091493898404 1106 14-15 0.09749096521156275 1106 16-17 0.12637143789518035 1106 18-19 0.011092636707317638 1106 20-21 0.16403881339249438 1106 22-23 0.12412598266704578 1106 24-25 0.17785178850194683 1106 26-27 0.19734046462663457 1106 28-29 0.1367653346968467 1106 30-31 0.16383542208080826 1106 32-33 0.17943223825360377 1106 34-35 0.21740676520981594 1106 36-37 0.19247703485533663 1106 38-39 0.20444651674772274 1106 40-41 0.24640336144565111 1106 42-43 0.22475333633491346 1106 44-45 0.21617653505708034 1106 46-47 0.27391156673756 1106 48-49 0.15051050776279595 1106 50-51 0.136522052387825 1106 52-53 0.12835793433174558 1106 54-55 0.06521564386836332 1106 56-57 0.1074288975010802 1106 58-59 0.2248447945502221 1106 60-61 0.05355679902653776 1106 62-63 0.13175090105744403 1106 64-65 0.1434752535607018 1106 66-67 0.08681734460031265 1106 68-69 0.15513490796179497 1106 70-71 0.1349556334253279 1106 72-73 0.3037998609063237 1106 74-75 0.21803501714632034 1106 76-77 0.36854810557559503 1106 78-79 0.5052285451360454 1106 80-81 0.4166689419720093 1106 82-83 0.5451999294710426 1106 84-85 0.5283888509298365 1106 86-87 0.5554586595095152 1106 88-89 0.8436838330339782 1106 90-91 0.7168947208376473 1106 92-93 0.7216206324081007 1106 94-95 0.5833375552631246 1106 96-97 0.6308829473802398 1106 98-99 0.7004531093800743 1106 100-101 0.6294815155624853 1107 1 0.0519360080701432 1107 2 0.031015108473020803 1107 3 0.055665033072884285 1107 4 0.024633817367920585 1107 5 0.052075943167224636 1107 6 0.04960216109390103 1107 7 0.06066153900999893 1107 8 0.0645209385843799 1107 9 0.06812532084429535 1107 10-11 0.0449697347949396 1107 12-13 0.05670971946859993 1107 14-15 0.130090100759638 1107 16-17 0.10793901089613911 1107 18-19 0.12609741129645613 1107 20-21 0.06634192441426023 1107 22-23 0.1411888564671102 1107 24-25 0.10329729715723346 1107 26-27 0.17433986870047136 1107 28-29 0.09784525342112715 1107 30-31 0.0552000570939839 1107 32-33 0.11110355984551745 1107 34-35 0.2666478568316748 1107 36-37 0.14494923095094236 1107 38-39 0.03735294916035059 1107 40-41 0.02292746055606898 1107 42-43 0.0775918834238496 1107 44-45 0.11881979068068205 1107 46-47 0.05571374079107727 1107 48-49 0.09607749919725705 1107 50-51 0.024699738901936996 1107 52-53 0.06287695730003406 1107 54-55 0.08522314679414933 1107 56-57 0.07131882347699303 1107 58-59 7.119389151313271E-4 1107 60-61 0.058728073949538384 1107 62-63 0.13844430063669932 1107 64-65 0.20962036517121163 1107 66-67 0.14948562194290815 1107 68-69 0.2612205346535461 1107 70-71 0.20598213418997346 1107 72-73 0.2960456409593135 1107 74-75 0.330335687024764 1107 76-77 0.20640862295438112 1107 78-79 0.47575532950410704 1107 80-81 0.4520565452718621 1107 82-83 0.4796345893982341 1107 84-85 0.3595972527707474 1107 86-87 0.3903198015337992 1107 88-89 0.4725277362785434 1107 90-91 0.6712202113997563 1107 92-93 0.5968315400108288 1107 94-95 0.6757070079444354 1107 96-97 0.6505933847038818 1107 98-99 0.6502220982441536 1107 100-101 0.7138535314442755 1108 1 0.028541180902699637 1108 2 -0.05608276387579281 1108 3 0.03745237436859128 1108 4 -0.011177891881821722 1108 5 0.05940955830213568 1108 6 0.051899311991256525 1108 7 0.003605340779337496 1108 8 0.056261245620007116 1108 9 0.12335116362562104 1108 10-11 0.1537489008888926 1108 12-13 0.10406544026235309 1108 14-15 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5 47.0 6 108.0 7 268.0 8 594.0 9 1197.0 10 2009.0 11 3029.0 12 4104.0 13 5990.0 14 7445.0 15 9595.0 16 11853.0 17 16125.0 18 15840.0 19 12518.0 20 12693.0 21 13243.0 22 14404.0 23 16523.0 24 19050.0 25 22680.0 26 27959.0 27 33903.0 28 41705.0 29 51190.0 30 62908.0 31 77447.0 32 96525.0 33 124690.0 34 169630.0 35 243178.0 36 390883.0 37 679290.0 38 633654.0 39 59513.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.04744115092533 19.274011480301983 14.829326548337527 23.84922082043516 2 15.466262477787481 20.11478159122305 40.021881997528645 24.397073933460824 3 19.27989281777043 23.688737086723986 29.65992683098638 27.371443264519208 4 13.38875662298248 16.697428466237145 36.452992743130125 33.46082216765024 5 14.355922869602242 36.89458852579671 34.18099181038656 14.568496794214491 6 32.871299084849475 35.65093043599863 17.256068298482106 14.221702180669793 7 29.268472955121084 31.943309456069617 20.470841179934492 18.317376408874804 8 26.008971410785055 35.98450424228292 19.548507697042947 18.458016649889082 9 26.072646427380462 17.284869532146516 20.24029250949313 36.40219153097989 10-11 25.14179402537493 26.51320120160135 27.24756325948072 21.097441513543 12-13 25.63875410721207 23.514819275728787 27.861341839066338 22.985084777992803 14-15 22.529983872688803 25.36014914509635 25.67008153878319 26.439785443431656 16-17 22.702055678077425 28.536086058758098 26.323006275421406 22.43885198774307 18-19 22.554957438369698 27.332492198509208 28.231108038979517 21.881442324141574 20-21 23.077890680927094 26.648497599261024 28.110194908011287 22.163416811800595 22-23 22.79667959946159 26.444650795451903 28.039770686659583 22.718898918426923 24-25 22.7658026981579 26.64786603456568 28.094886630592214 22.491444636684204 26-27 22.57627288030256 26.89282463579073 28.000226248190796 22.530676235715912 28-29 22.50051269665951 27.00028905334509 28.137417278866383 22.36178097112902 30-31 22.734895748209205 26.6747310120927 28.021952092097322 22.56842114760077 32-33 22.51517356560314 26.783898097765267 27.947971091889467 22.752957244742124 34-35 22.99055392825205 26.807171532903705 27.711056599046337 22.491217939797906 36-37 22.927026256886485 26.653407085343645 27.77313655795337 22.6464300998165 38-39 22.608841705487475 26.889495338726604 27.93647730995386 22.565185645832063 40-41 22.836083268547313 26.655410095513105 27.97104064860062 22.537465987338962 42-43 22.684219393586275 26.743414734358907 27.75685151662425 22.815514355430576 44-45 22.79940717668765 26.858983768147926 27.60388201414184 22.73772704102258 46-47 22.82435401421291 27.03520505209128 27.265476479852204 22.874964453843607 48-49 22.886275726354967 26.96338530394497 27.380187735463096 22.770151234236963 50-51 22.846838856403643 27.058932529119613 27.306952791308 22.787275823168756 52-53 22.999259842995105 26.98059664358057 27.25053013355097 22.769613379873352 54-55 22.692457303906107 26.892700830481836 27.33492121821584 23.079920647396214 56-57 22.777559744342206 27.191487604538935 27.20089138083177 22.830061270287086 58-59 22.857836370664785 26.85368133732805 27.310929326956778 22.97755296505039 60-61 22.882928650645923 26.932323396364172 27.439460691437606 22.7452872615523 62-63 22.774814333631067 26.957000911241725 27.431238726593477 22.836946028533735 64-65 22.598604805054585 26.783270083362265 27.386274539401235 23.231850572181916 66-67 22.47951902633786 27.04885209733707 27.31489915577685 23.15672972054822 68-69 22.523422860712056 26.880682212506073 27.48384690124228 23.112048025539593 70-71 22.54020981872226 26.836295522865576 27.357684602895183 23.26581005551698 72-73 22.701236475131523 26.723918661152098 27.26670046474087 23.308144398975507 74-75 22.546750821789416 26.919767050070075 27.116864649122896 23.416617479017606 76-77 22.60934500904408 26.75736078109677 27.245833210873204 23.387460998985944 78-79 22.527873868640697 26.860030515427574 27.243173673868014 23.368921942063718 80-81 22.447597582748195 27.039213542995057 27.24235152865325 23.270837345603507 82-83 22.649880058476914 26.903319116627994 27.136938761984176 23.309862062910916 84-85 22.55938172426393 26.748781412863604 27.100260564413958 23.59157629845851 86-87 22.52679811872578 26.645207445410723 27.33447188900489 23.493522546858607 88-89 22.46466223124908 26.688303954263937 27.20642522615893 23.640608588328053 90-91 22.365748827357532 26.807694296805444 27.303445584635643 23.52311129120138 92-93 22.435276167395546 26.540492973019493 27.313840797190387 23.710390062394577 94-95 22.271321201303135 26.454041811417472 27.29316416480485 23.981472822474544 96-97 22.304872509430666 26.36924708370159 27.36563096736404 23.960249439503702 98-99 22.129167633937012 26.397788065254574 27.194680030439088 24.278364270369327 100-101 22.140834357284362 25.99564995761346 27.74198238538197 24.12153329972021 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 94.0 1 75.0 2 66.5 3 89.0 4 91.5 5 91.5 6 168.0 7 400.0 8 664.5 9 831.0 10 929.5 11 943.0 12 1075.5 13 1308.5 14 1447.0 15 1586.0 16 1782.0 17 1961.5 18 2064.0 19 2255.5 20 2551.5 21 3042.0 22 3792.5 23 4700.0 24 5919.0 25 7728.5 26 10481.5 27 14103.5 28 17696.5 29 22113.0 30 27646.0 31 33463.5 32 39588.0 33 45051.0 34 50854.5 35 57392.5 36 64955.0 37 73205.5 38 78836.5 39 82024.0 40 85553.5 41 92229.0 42 98641.0 43 103586.0 44 109122.0 45 114080.5 46 115369.5 47 116757.0 48 124875.5 49 126222.5 50 119611.0 51 114067.5 52 107907.0 53 105356.5 54 102842.0 55 94150.0 56 83113.0 57 73786.0 58 65738.0 59 56387.5 60 46363.5 61 37649.5 62 30433.0 63 23278.5 64 17609.5 65 13660.0 66 10127.0 67 8122.5 68 6092.0 69 4042.5 70 2985.5 71 2211.5 72 1721.5 73 1477.5 74 1199.0 75 946.0 76 644.0 77 352.5 78 199.0 79 122.0 80 70.0 81 39.0 82 24.0 83 14.0 84 9.0 85 5.5 86 2.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27860856035125015 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022034678767348837 16-17 7.807563342761399E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.04100844570152E-4 26-27 0.0010410084457015201 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003261826463198096 36-37 0.021999978485825456 38-39 0.00711355771229372 40-41 0.018772852304150746 42-43 0.004910089835558836 44-45 6.246050674209119E-4 46-47 1.5615126685522798E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 5.205042228507601E-4 60-61 0.001977916046832888 62-63 0.001353310979411976 64-65 5.2050422285076E-5 66-67 0.0 68-69 7.28705911991064E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.23372374620075292 78-79 0.0 80-81 0.0012492101348418239 82-83 0.0 84-85 0.0 86-87 0.10505510231204505 88-89 0.36050122474643637 90-91 0.36162898389594633 92-93 0.0 94-95 1.7350140761692E-5 96-97 0.0 98-99 0.0 100-101 3.4700281523384E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2881821.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.01863126712577 #Duplication Level Percentage of deduplicated Percentage of total 1 72.23569384619462 37.576019225101845 2 14.8548731655345 15.454603394357209 3 5.391274482811879 8.41340158143766 4 2.5794670673214535 5.367213849627558 5 1.379763045787191 3.5886692557405127 6 0.8348828590716775 2.605767815837599 7 0.5575573582188508 2.0302359439221407 8 0.37404679262011853 1.5565921745565603 9 0.2752849267218026 1.2887950586885266 >10 1.3691778017865306 12.588473525920188 >50 0.09311833170056771 3.310761972665842 >100 0.051925288462932956 4.8190068632327066 >500 0.002201275326068278 0.7363515270735905 >1k 7.3375844191755E-4 0.6641078118381919 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4471 0.15514495869104986 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3567 0.12377590419391073 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.4700281523384E-5 0.0 0.0 0.0 6 0.0 3.4700281523384E-5 0.0 0.0 0.0 7 0.0 3.4700281523384E-5 0.0 3.4700281523384E-5 0.0 8 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 0.0 9 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 0.0 10-11 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 0.0 12-13 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 1.7350140761692E-5 14-15 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 3.4700281523384E-5 16-17 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 3.4700281523384E-5 18-19 0.0 3.4700281523384E-5 0.0 6.9400563046768E-5 3.4700281523384E-5 20-21 0.0 3.4700281523384E-5 0.0 8.675070380845999E-5 3.4700281523384E-5 22-23 0.0 6.9400563046768E-5 0.0 1.04100844570152E-4 3.4700281523384E-5 24-25 0.0 6.9400563046768E-5 0.0 1.7350140761691999E-4 3.4700281523384E-5 26-27 0.0 6.9400563046768E-5 0.0 3.8170309675722403E-4 3.4700281523384E-5 28-29 0.0 6.9400563046768E-5 0.0 0.001682963653884124 3.4700281523384E-5 30-31 0.0 6.9400563046768E-5 0.0 0.006558353207919575 3.4700281523384E-5 32-33 0.0 8.675070380845999E-5 0.0 0.017454241606262154 3.4700281523384E-5 34-35 0.0 1.04100844570152E-4 0.0 0.033294920121686944 3.4700281523384E-5 36-37 3.4700281523384E-5 1.04100844570152E-4 0.0 0.059493632671841865 3.4700281523384E-5 38-39 3.4700281523384E-5 1.38801126093536E-4 0.0 0.09905195360849962 3.4700281523384E-5 40-41 3.4700281523384E-5 1.38801126093536E-4 0.0 0.1440235184628053 3.4700281523384E-5 42-43 3.4700281523384E-5 1.38801126093536E-4 0.0 0.19034839429652292 3.4700281523384E-5 44-45 3.4700281523384E-5 1.38801126093536E-4 0.0 0.24878366838190158 3.4700281523384E-5 46-47 3.4700281523384E-5 1.38801126093536E-4 0.0 0.3220186125370035 3.4700281523384E-5 48-49 3.4700281523384E-5 1.38801126093536E-4 0.0 0.40030244765375783 3.4700281523384E-5 50-51 3.4700281523384E-5 1.38801126093536E-4 0.0 0.4846067816148193 3.4700281523384E-5 52-53 3.4700281523384E-5 1.38801126093536E-4 0.0 0.5697092220509186 3.4700281523384E-5 54-55 3.4700281523384E-5 1.38801126093536E-4 0.0 0.6702532877649237 3.4700281523384E-5 56-57 3.4700281523384E-5 1.56151266855228E-4 0.0 0.7869850348095875 5.2050422285076E-5 58-59 3.4700281523384E-5 1.7350140761691999E-4 0.0 0.9160527319358143 6.9400563046768E-5 60-61 3.4700281523384E-5 1.7350140761691999E-4 0.0 1.0607529058883256 6.9400563046768E-5 62-63 3.4700281523384E-5 1.7350140761691999E-4 0.0 1.2100855674242084 6.9400563046768E-5 64-65 3.4700281523384E-5 1.7350140761691999E-4 0.0 1.3748078038157123 6.9400563046768E-5 66-67 3.4700281523384E-5 1.7350140761691999E-4 0.0 1.5622760747457942 6.9400563046768E-5 68-69 3.4700281523384E-5 1.7350140761691999E-4 0.0 1.7758736576629846 6.9400563046768E-5 70-71 3.4700281523384E-5 2.42901970663688E-4 0.0 2.012512227511702 6.9400563046768E-5 72-73 3.4700281523384E-5 2.6025211142538E-4 0.0 2.2579646688673582 6.9400563046768E-5 74-75 3.4700281523384E-5 2.77602252187072E-4 0.0 2.5147641022811618 6.9400563046768E-5 76-77 3.4700281523384E-5 2.77602252187072E-4 0.0 2.7975713966967417 6.9400563046768E-5 78-79 3.4700281523384E-5 2.77602252187072E-4 0.0 3.119971712330502 6.9400563046768E-5 80-81 3.4700281523384E-5 2.77602252187072E-4 0.0 3.476343603575656 6.9400563046768E-5 82-83 3.4700281523384E-5 3.12302533710456E-4 0.0 3.8383022401460742 1.04100844570152E-4 84-85 3.4700281523384E-5 3.6435295599553195E-4 0.0 4.21735076536676 1.04100844570152E-4 86-87 3.4700281523384E-5 3.81703096757224E-4 0.0 4.625061723125759 1.04100844570152E-4 88-89 3.4700281523384E-5 3.81703096757224E-4 0.0 5.068722172542985 1.04100844570152E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6230 0.0 30.163868 1 GTATCAA 8810 0.0 29.538567 1 ATCAACG 10400 0.0 24.73018 3 TCAACGC 10560 0.0 24.355482 4 CAACGCA 10760 0.0 23.946878 5 TATCAAC 11045 0.0 23.672668 2 GTACATG 8675 0.0 23.581802 1 AACGCAG 11065 0.0 23.286797 6 TACATGG 8870 0.0 22.25518 2 ACATGGG 8960 0.0 21.078339 3 ACGCAGA 12425 0.0 20.585135 7 CGCAGAG 12640 0.0 20.00974 8 GTATAGG 815 0.0 19.847202 1 GAGTACT 5985 0.0 19.544369 12-13 CATGGGG 5300 0.0 18.7125 4 GTACTTT 6360 0.0 18.168152 14-15 GCAGAGT 13900 0.0 17.752108 9 ATTCGCG 365 0.0 16.920502 80-81 AGTACTT 6455 0.0 16.90832 12-13 AGGCGTA 170 0.0018003912 16.748028 5 >>END_MODULE