FastQCFastQC Report
Fri 27 May 2016
SRR939255_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939255_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178506
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.2718696383387102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.18387687461922128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17090.1450141110864094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42400.037.946661
GGTATCA24800.034.2563481
ATCAACG51200.030.9308073
TCAACGC51800.030.6627644
CAACGCA52750.030.1105445
TATCAAC53200.029.7679922
AACGCAG54500.029.0406446
GTACATG48700.027.677641
TACATGG48250.027.3188342
GTGTACG908.990335E-426.3673931
ACATGGG49700.025.3769823
ACGCAGA61950.025.0128237
CGCAGAG63200.024.4431298
CATGGGG26300.022.7149124
GCAGAGT69200.021.8444379
GTACTAG2257.21393E-821.0939141
GTACTTT32500.020.77988414-15
GAGTACT32900.020.45304312-13
CATGGGA26150.020.306854
AGAGTAC60150.019.10354210-11
Produced by FastQC (version 0.11.5)