##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939255_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178506 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.915255416603735 33.0 31.0 34.0 30.0 34.0 2 31.986355606165773 33.0 31.0 34.0 30.0 34.0 3 32.040326481154956 34.0 31.0 34.0 30.0 34.0 4 35.532762667309285 37.0 35.0 37.0 33.0 37.0 5 35.561086663962676 37.0 35.0 37.0 33.0 37.0 6 35.64542734614843 37.0 35.0 37.0 33.0 37.0 7 35.57891771446221 37.0 35.0 37.0 33.0 37.0 8 35.56528010888362 37.0 35.0 37.0 33.0 37.0 9 37.20361287935742 39.0 37.0 39.0 34.0 39.0 10-11 37.09198807642897 39.0 37.0 39.0 33.0 39.0 12-13 36.99537040965426 39.0 37.0 39.0 33.0 39.0 14-15 38.23459320529552 40.0 38.0 41.0 33.0 41.0 16-17 38.150187610415216 40.0 38.0 41.0 33.0 41.0 18-19 38.06074852397866 40.0 38.0 41.0 33.0 41.0 20-21 38.02896930520507 40.0 38.0 41.0 33.0 41.0 22-23 38.04519832737381 40.0 38.0 41.0 33.0 41.0 24-25 37.96614272646893 40.0 38.0 41.0 33.0 41.0 26-27 37.87708420661414 40.0 38.0 41.0 33.0 41.0 28-29 37.7267502244367 40.0 38.0 41.0 32.5 41.0 30-31 37.55173753888398 40.0 37.5 41.0 32.0 41.0 32-33 37.338366542045605 40.0 37.0 41.0 31.5 41.0 34-35 37.06034971395988 40.0 37.0 41.0 31.0 41.0 36-37 36.824412858313835 40.0 36.0 41.0 30.0 41.0 38-39 36.23021393187646 39.5 35.5 40.5 29.5 41.0 40-41 36.216113452116495 39.0 35.0 40.0 30.0 41.0 42-43 36.40154229168116 39.0 36.0 41.0 30.0 41.0 44-45 36.31552151622478 40.0 36.0 41.0 30.0 41.0 46-47 36.06913117116078 39.5 35.5 41.0 28.5 41.0 48-49 35.83929992719595 39.0 35.0 41.0 27.5 41.0 50-51 34.732101915476036 38.0 34.0 40.0 25.5 40.5 52-53 34.84271611684625 38.5 34.5 39.5 26.0 40.5 54-55 35.32268779284959 39.0 35.0 40.5 26.0 41.0 56-57 35.24089822198614 39.0 35.0 41.0 26.0 41.0 58-59 35.02773553974269 38.5 35.0 40.5 26.0 41.0 60-61 34.7213565310656 38.0 34.0 40.0 26.0 41.0 62-63 34.32852441990113 38.0 34.0 40.0 24.5 41.0 64-65 33.94228031083422 37.0 34.0 40.0 23.0 41.0 66-67 33.57582396695477 37.0 33.5 39.5 22.5 41.0 68-69 33.17112598493347 36.0 33.0 39.0 22.0 41.0 70-71 32.756868017642674 35.5 32.5 39.0 20.5 40.0 72-73 32.30881853804732 35.0 32.0 38.0 19.5 40.0 74-75 31.839774256558727 35.0 32.0 37.0 18.0 39.5 76-77 31.361896757419988 35.0 31.0 37.0 15.5 39.0 78-79 30.959696429207828 35.0 31.0 36.0 12.0 39.0 80-81 30.537245885892816 35.0 31.0 36.0 7.5 37.5 82-83 30.093781872981555 34.0 30.0 35.5 4.5 37.0 84-85 29.66025374499578 34.0 30.0 35.0 2.0 37.0 86-87 29.20850678740711 34.0 29.0 35.0 2.0 36.0 88-89 28.772367726596215 34.0 29.0 35.0 2.0 36.0 90-91 28.353879827510426 34.0 29.0 35.0 2.0 35.5 92-93 27.890150325921127 34.0 27.5 35.0 2.0 35.0 94-95 27.392793927226506 34.0 27.0 35.0 2.0 35.0 96-97 26.763609604024076 33.0 25.5 35.0 2.0 35.0 98-99 25.934155193100416 33.0 24.5 35.0 2.0 35.0 100-101 23.10324809547003 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15110006795821107 1101 2 0.27726392436068537 1101 3 0.2878172960207692 1101 4 0.24478450252321693 1101 5 0.13147951475770014 1101 6 0.1761655660919601 1101 7 0.1721538963522775 1101 8 0.1805738813224096 1101 9 0.3659089533392148 1101 10-11 0.3857306295739633 1101 12-13 0.369429762619383 1101 14-15 0.4945961332305302 1101 16-17 0.5343006262343835 1101 18-19 0.5317402726612812 1101 20-21 0.48950527899531693 1101 22-23 0.6238874038293858 1101 24-25 0.5770339181947648 1101 26-27 0.5895193574560054 1101 28-29 0.5613137973689462 1101 30-31 0.6491387206909707 1101 32-33 0.5495469432804185 1101 34-35 0.3495286264795183 1101 36-37 0.14358621686033501 1101 38-39 0.6657484360720929 1101 40-41 0.5012729010425261 1101 42-43 0.32474853459243036 1101 44-45 0.28579257919967915 1101 46-47 0.23020838011779432 1101 48-49 0.15449415137114642 1101 50-51 0.1891447693544137 1101 52-53 0.1471203385877473 1101 54-55 0.26769896387469316 1101 56-57 0.3526828822873256 1101 58-59 0.33914980259322647 1101 60-61 0.31082676168953327 1101 62-63 0.4621812223053752 1101 64-65 0.26886206974597116 1101 66-67 0.2068077555858565 1101 68-69 0.2729652622002092 1101 70-71 0.326152827435088 1101 72-73 0.2571791735170166 1101 74-75 0.0500430472704565 1101 76-77 4.484543251592754E-5 1101 78-79 -0.12248982484386772 1101 80-81 0.0939453846996976 1101 82-83 -0.09551466396857577 1101 84-85 -0.06124834986350791 1101 86-87 0.0247239928409968 1101 88-89 0.17745073625945196 1101 90-91 -0.05635764842275037 1101 92-93 0.1374048488634756 1101 94-95 0.2224375744961975 1101 96-97 0.1488155664427282 1101 98-99 0.31215825129663344 1101 100-101 0.04351018144759067 1102 1 0.23648011922402645 1102 2 0.2624781957290985 1102 3 0.31886102855345655 1102 4 0.2483945555251239 1102 5 0.3416268103667406 1102 6 0.36373683953347324 1102 7 0.2780317638849539 1102 8 0.24225966203679405 1102 9 0.32235173204177414 1102 10-11 0.3011199059634393 1102 12-13 0.18112150051693732 1102 14-15 0.439831748335223 1102 16-17 0.2237552205842519 1102 18-19 0.3200279399304762 1102 20-21 0.28495262941044786 1102 22-23 0.30053575641292696 1102 24-25 0.2757672508472737 1102 26-27 0.4768579603433736 1102 28-29 0.4658754678311894 1102 30-31 0.422308759123311 1102 32-33 0.30960181744769244 1102 34-35 0.02842964532804615 1102 36-37 0.25421621451150145 1102 38-39 0.8147963079960547 1102 40-41 0.5773095360169833 1102 42-43 0.450486799792607 1102 44-45 0.665235361306344 1102 46-47 0.5614209562420953 1102 48-49 0.519082271726667 1102 50-51 0.5066039115881154 1102 52-53 0.42377618020157115 1102 54-55 0.5494898973823155 1102 56-57 0.45126490248728146 1102 58-59 0.4053843181721106 1102 60-61 0.4874283078338735 1102 62-63 0.35883941275142206 1102 64-65 0.26712087020757025 1102 66-67 0.33094183646057473 1102 68-69 0.3127541396286091 1102 70-71 0.45705171531408695 1102 72-73 0.3734250928465457 1102 74-75 0.19866285303223918 1102 76-77 0.2122703469422369 1102 78-79 0.22891249607469533 1102 80-81 0.2629319486985686 1102 82-83 -0.07378711488989964 1102 84-85 0.0884692796541664 1102 86-87 0.03999055125713724 1102 88-89 0.12981880167272664 1102 90-91 0.1340747749934046 1102 92-93 0.11884737225818398 1102 94-95 0.21149763311487746 1102 96-97 0.48251994718502544 1102 98-99 0.39523202164892624 1102 100-101 0.0823756954844157 1103 1 0.21149879803488858 1103 2 0.3487251694228952 1103 3 0.3074970263916299 1103 4 0.14004728720591686 1103 5 0.1448419377469108 1103 6 0.21745167804859022 1103 7 0.20180357912651914 1103 8 0.1936727289027047 1103 9 0.34486118157170864 1103 10-11 0.355041524420983 1103 12-13 0.3713814960103008 1103 14-15 0.4897960849367138 1103 16-17 0.45523863159770883 1103 18-19 0.4645604275075854 1103 20-21 0.3899834558017048 1103 22-23 0.5357320575848235 1103 24-25 0.42319813077597956 1103 26-27 0.4641165547141455 1103 28-29 0.4404007041329763 1103 30-31 0.49316202075711146 1103 32-33 0.43687398254635923 1103 34-35 0.3158194572862172 1103 36-37 0.15939138872000314 1103 38-39 0.678588809702994 1103 40-41 0.5420684226412575 1103 42-43 0.4584327066340492 1103 44-45 0.5571528153632528 1103 46-47 0.49365922840549814 1103 48-49 0.41523782513620944 1103 50-51 0.4323147652902506 1103 52-53 0.4741077396832125 1103 54-55 0.2786522226020054 1103 56-57 0.4979911549841418 1103 58-59 0.5038585007517682 1103 60-61 0.4881373402460625 1103 62-63 0.3899305263812778 1103 64-65 0.4168715652626318 1103 66-67 0.41703040028451 1103 68-69 0.3077062015829668 1103 70-71 0.21306197214138933 1103 72-73 0.29506218684713303 1103 74-75 0.31775361426367255 1103 76-77 0.472534374686834 1103 78-79 0.35154757225544486 1103 80-81 0.40664019668540874 1103 82-83 0.48647259258552467 1103 84-85 0.4819822684163846 1103 86-87 0.34703250530641583 1103 88-89 0.2292811399379424 1103 90-91 0.06462856573311981 1103 92-93 0.1395283313918263 1103 94-95 0.22613953362670003 1103 96-97 0.129499740184837 1103 98-99 0.2983796342342906 1103 100-101 0.23636660767113327 1104 1 0.40448225463099163 1104 2 0.3483989472849096 1104 3 0.32626360480779937 1104 4 0.27527852335300196 1104 5 0.28332533186602404 1104 6 0.20317924985729263 1104 7 0.32957195973131803 1104 8 0.2869689058979219 1104 9 0.47471771930655393 1104 10-11 0.5625062249213855 1104 12-13 0.5083218342582185 1104 14-15 0.5632807663334134 1104 16-17 0.559532016835874 1104 18-19 0.588646858763191 1104 20-21 0.6389668419539518 1104 22-23 0.6936462150385836 1104 24-25 0.6899249822124105 1104 26-27 0.6798215090136992 1104 28-29 0.5893014026173446 1104 30-31 0.5988743465029174 1104 32-33 0.6093222167956611 1104 34-35 0.7479556084855616 1104 36-37 0.732502046791403 1104 38-39 1.303280303355912 1104 40-41 0.9725893968797692 1104 42-43 0.9308962875803317 1104 44-45 0.6817868637296627 1104 46-47 0.8137208835509497 1104 48-49 0.808354789609858 1104 50-51 0.8755416305638661 1104 52-53 0.8392861680975727 1104 54-55 0.8877705925982937 1104 56-57 0.826566453175495 1104 58-59 0.6455445744209456 1104 60-61 0.8538621278452609 1104 62-63 0.7902491171016379 1104 64-65 0.8250689657166603 1104 66-67 0.7642699655788263 1104 68-69 0.6957457574847723 1104 70-71 0.875570075281253 1104 72-73 0.7859271283661542 1104 74-75 0.7665463826987917 1104 76-77 0.8457953968439433 1104 78-79 0.7328756737348492 1104 80-81 0.6574802815870235 1104 82-83 0.8218603985864981 1104 84-85 0.6725484105838255 1104 86-87 0.5726255964414761 1104 88-89 0.764523613557138 1104 90-91 0.5339213345875429 1104 92-93 0.6191046451487558 1104 94-95 0.6163045651293615 1104 96-97 0.6825540263998988 1104 98-99 0.5041071389536107 1104 100-101 0.36885640379487583 1105 1 -0.010309844823570558 1105 2 0.10937648098989072 1105 3 0.1894747794274707 1105 4 0.13955333650347512 1105 5 0.17843378260483433 1105 6 0.18147959825237336 1105 7 0.18776035284514592 1105 8 0.192815908266752 1105 9 0.11071416170098303 1105 10-11 0.15327625011449442 1105 12-13 0.1809650987652347 1105 14-15 0.2006746697759425 1105 16-17 0.23805618075500234 1105 18-19 0.1462163932411542 1105 20-21 0.2770925413774634 1105 22-23 0.28645639872411977 1105 24-25 0.14347256914684436 1105 26-27 0.10827063216529353 1105 28-29 0.25699672838931065 1105 30-31 0.3364157971221289 1105 32-33 0.36382842478569444 1105 34-35 0.3077755740024415 1105 36-37 0.245660118683233 1105 38-39 0.5396088258962592 1105 40-41 0.3896806345732813 1105 42-43 0.2853417501680511 1105 44-45 0.13172338597222222 1105 46-47 0.2732325285317003 1105 48-49 0.250942068556391 1105 50-51 0.1921819900625934 1105 52-53 0.19369480329014976 1105 54-55 0.16196955715450656 1105 56-57 0.3130749547598626 1105 58-59 0.32431861023618325 1105 60-61 0.4232203887051824 1105 62-63 0.4081379648952037 1105 64-65 0.4362219641398184 1105 66-67 0.4022602352266418 1105 68-69 0.21400343362070373 1105 70-71 0.2645275590435432 1105 72-73 0.36106095436819885 1105 74-75 0.48133681898066527 1105 76-77 0.3069314071461129 1105 78-79 0.22481285015865993 1105 80-81 0.17970541656140782 1105 82-83 0.3173072653671589 1105 84-85 0.4878265524556191 1105 86-87 0.3775659321014011 1105 88-89 0.3657106436613553 1105 90-91 0.5752373160138653 1105 92-93 0.6755052068259317 1105 94-95 0.7110836284780255 1105 96-97 0.9046374749013388 1105 98-99 0.7018302555491438 1105 100-101 0.43878568727632583 1106 1 0.20168194581101773 1106 2 0.1268841791169315 1106 3 0.09884373987002704 1106 4 0.10926763403829653 1106 5 0.05976959234141077 1106 6 0.1612901149873025 1106 7 0.22326704031596734 1106 8 0.2964606241896135 1106 9 0.18042949353310433 1106 10-11 0.34036474754648083 1106 12-13 0.4180563296972295 1106 14-15 0.542307130753386 1106 16-17 0.43040666569267216 1106 18-19 0.3350052015533578 1106 20-21 0.39393710717946107 1106 22-23 0.5010069669966128 1106 24-25 0.46425618233147503 1106 26-27 0.5346881366127718 1106 28-29 0.47548759997686574 1106 30-31 0.4257735428268461 1106 32-33 0.34747249057882357 1106 34-35 0.1724307540588299 1106 36-37 0.31611017098911987 1106 38-39 0.7802048471023113 1106 40-41 0.6477703170144622 1106 42-43 0.4541841350803182 1106 44-45 0.3006813905467709 1106 46-47 0.4214296005837994 1106 48-49 0.3863422972838535 1106 50-51 0.20328147764216453 1106 52-53 0.2744874591883004 1106 54-55 0.38150543840535533 1106 56-57 0.3966129312015312 1106 58-59 0.37709289110404853 1106 60-61 0.5089968800141307 1106 62-63 0.33770305765689557 1106 64-65 0.37648353792658185 1106 66-67 0.22298622327607376 1106 68-69 0.22743119829737424 1106 70-71 0.22659544440794122 1106 72-73 0.23503810821844695 1106 74-75 0.22812643404475352 1106 76-77 0.31782292709031523 1106 78-79 0.4945498283556766 1106 80-81 0.4155492110731309 1106 82-83 0.3281747748383701 1106 84-85 0.5428703771097219 1106 86-87 0.7014823579245544 1106 88-89 0.6012478429024739 1106 90-91 0.4738514315123652 1106 92-93 0.43791049474474875 1106 94-95 0.3092162785841168 1106 96-97 0.24827942777284306 1106 98-99 0.2939828203109336 1106 100-101 -0.007819084084868422 1107 1 -0.125053620210263 1107 2 -0.05714686919983336 1107 3 0.06704537950024303 1107 4 0.09200790963571848 1107 5 0.020230956374206244 1107 6 0.025270578967763413 1107 7 0.03508894856644673 1107 8 -0.0084887914740861 1107 9 0.13285841381917152 1107 10-11 0.218192673588689 1107 12-13 0.2997195077889714 1107 14-15 0.45704854013764873 1107 16-17 0.3517785477788493 1107 18-19 0.25590176530706543 1107 20-21 0.1979053611477113 1107 22-23 0.14566726701404775 1107 24-25 0.2938174940356433 1107 26-27 0.31327482769946613 1107 28-29 0.25343980650050213 1107 30-31 0.28852210378968834 1107 32-33 0.401453826703019 1107 34-35 0.3356851583846634 1107 36-37 0.256553656075468 1107 38-39 0.7662200224031963 1107 40-41 0.4971423735293783 1107 42-43 0.1684567672100954 1107 44-45 0.274807066458159 1107 46-47 0.283909977349893 1107 48-49 0.18247652020378524 1107 50-51 0.07695145845499951 1107 52-53 0.21580526836325475 1107 54-55 0.22158698384404119 1107 56-57 0.03543640931072645 1107 58-59 0.025880611320339142 1107 60-61 0.1825055142370502 1107 62-63 0.2698371933624628 1107 64-65 0.2939896429326936 1107 66-67 0.061347465208747565 1107 68-69 0.150859159225341 1107 70-71 0.07310686949079326 1107 72-73 0.20704883558631337 1107 74-75 0.287499798775265 1107 76-77 0.26969585574896016 1107 78-79 0.20759927890512486 1107 80-81 0.20926759586294352 1107 82-83 0.1280875604654419 1107 84-85 0.1003359274324147 1107 86-87 0.2659621241900325 1107 88-89 0.31632066690387006 1107 90-91 0.05843847145654735 1107 92-93 0.165716617193727 1107 94-95 0.3293802416052216 1107 96-97 0.4363070747290614 1107 98-99 0.7136954489521337 1107 100-101 0.6157963877448864 1108 1 0.026136146095780077 1108 2 0.0378555444072326 1108 3 0.07001127665218121 1108 4 -0.08156451277391596 1108 5 0.0469353888925923 1108 6 0.07186597323757837 1108 7 0.16051984841595157 1108 8 0.11098759776974276 1108 9 0.17799712523694922 1108 10-11 0.2635869434015632 1108 12-13 0.3155804556266659 1108 14-15 0.24483639827434445 1108 16-17 0.33334923807046835 1108 18-19 0.2956127003668385 1108 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-0.5065223477909271 2316 60-61 -0.5416330339646862 2316 62-63 -0.9057372648350679 2316 64-65 -1.058208652689558 2316 66-67 -1.1481000878097163 2316 68-69 -1.2066836857164773 2316 70-71 -0.9658975704320909 2316 72-73 -0.8052168438726071 2316 74-75 -0.7673872563689557 2316 76-77 -0.6566315681537738 2316 78-79 -0.5685231047117476 2316 80-81 -0.6253942603639366 2316 82-83 -0.644593097924254 2316 84-85 -0.3385181077478485 2316 86-87 -0.2987191370746203 2316 88-89 -0.5095790925741355 2316 90-91 -0.42449106125255653 2316 92-93 -0.6702373382312032 2316 94-95 -0.7985463709282996 2316 96-97 -0.6248021523977982 2316 98-99 -0.9057230907088787 2316 100-101 -0.0761596275028289 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4900.0 3 1159.0 4 1130.0 5 1269.0 6 1464.0 7 1782.0 8 2141.0 9 2643.0 10 3015.0 11 3693.0 12 4684.0 13 5934.0 14 6127.0 15 6231.0 16 7429.0 17 9334.0 18 8456.0 19 6577.0 20 6597.0 21 6989.0 22 7716.0 23 8513.0 24 9620.0 25 11108.0 26 12754.0 27 14804.0 28 17511.0 29 21228.0 30 25679.0 31 31376.0 32 39301.0 33 51359.0 34 70514.0 35 101460.0 36 159068.0 37 247173.0 38 227082.0 39 30672.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.338720122679234 18.758706728040323 14.549607394112996 23.352965755167446 2 14.866898108793666 19.91164509789254 41.47758570272494 23.74387109058885 3 19.106903932499264 24.48763262898227 29.429905763506014 26.975557675012453 4 12.65002669786519 16.356268556716806 37.42386006917638 33.56984467624162 5 14.006575042390827 37.12670987667968 34.095489480102245 14.771225600827236 6 31.99005473801523 35.9181274230321 17.897309308860354 14.194508530092317 7 28.87594314255396 32.360080166586094 20.70389592956623 18.060080761293708 8 25.998584405862562 35.79311932880785 19.78390522146911 18.424391043860478 9 26.65603387989936 17.14158742351489 20.18756643721487 36.014812259370885 10-11 25.01779927851325 26.622792931944733 27.42206124990867 20.937346539633346 12-13 25.830846700973343 23.775249495063193 27.98252525745937 22.411378546504096 14-15 22.769027115229637 25.282922040156897 26.16188251327468 25.78616833133879 16-17 22.390960505727833 28.4946748055187 26.684541900760593 22.42982278799287 18-19 22.328612201303926 27.09704335176866 28.889665878442393 21.684678568485023 20-21 23.144886986586616 26.42322788134484 28.69824220243994 21.7336429296286 22-23 22.690396619250958 26.268460848043084 28.648099858568827 22.393042674137135 24-25 22.462904719741317 26.434215520953465 28.82386272100209 22.27901703830313 26-27 22.349355270690264 26.756319477705727 28.607273343892892 22.287051907711124 28-29 22.1773158512973 26.823000334885368 28.810595976955806 22.18908783686153 30-31 22.451125953663247 26.621705575248996 28.613296936124428 22.31387153496333 32-33 22.271444222794337 26.67095328716929 28.566061695036844 22.49154079499953 34-35 22.71476151895242 26.826089714633365 28.25477487894898 22.20437388746523 36-37 22.79932327856107 26.501939244259525 28.35431286303552 22.344424614143886 38-39 22.435260449601753 26.69244090220335 28.491580833036473 22.38071781515842 40-41 22.615482936389586 26.632354820842743 28.421924928635338 22.330237314132333 42-43 22.600468783294268 26.587470701044108 28.16352013637332 22.648540379288303 44-45 22.518784334951437 26.859617195923917 28.15280624965372 22.468792219470927 46-47 22.612589651486505 26.9673887656693 27.785439933717143 22.634581649127053 48-49 22.75685686944401 26.85339545312137 27.799471868133274 22.590275809301342 50-51 22.67721656835805 27.111269666517913 27.61519455217261 22.596319212951418 52-53 22.724585444490717 27.063634585031032 27.56984348699334 22.641936483484915 54-55 22.550958699896313 26.948563772049717 27.658868107220286 22.841609420833688 56-57 22.572049332225703 27.20808571367713 27.556563784693378 22.663301169403795 58-59 22.603085460797896 26.898978569946806 27.764674511020022 22.733261458235273 60-61 22.705811774613895 26.97275002842042 27.841414455798898 22.480023741166782 62-63 22.566309820349275 26.96589415321034 27.845563801836914 22.62223222460347 64-65 22.46750938456758 26.810346701538144 27.804064465063664 22.91807944883061 66-67 22.334568814300603 27.07007713408201 27.645289790977078 22.950064260640303 68-69 22.41520804654397 26.91450952037663 27.851348856800218 22.818933576279186 70-71 22.51251474422945 26.87139280588175 27.699243722805498 22.9168487270833 72-73 22.505566960388474 26.900908105085115 27.574953622872105 23.0185713116543 74-75 22.447726862921208 27.173505764740934 27.40983307465496 22.968934297682893 76-77 22.44647908812657 26.946565601206242 27.51269355703428 23.09426175363291 78-79 22.50608272506083 27.032226724788117 27.52583293918127 22.935857610969784 80-81 22.471339425148017 27.27583393164924 27.395482910180174 22.857343733022564 82-83 22.629283526269226 27.10649856725709 27.36675320513367 22.897464701340013 84-85 22.615271975977777 26.97820663509048 27.29655392257555 23.109967466356192 86-87 22.557010684042975 26.981406270642534 27.483858870056977 22.977724175257514 88-89 22.53508400684142 26.792004592768947 27.484329562710947 23.18858183767869 90-91 22.508222941025018 27.018307873050407 27.49505941490514 22.97840977101944 92-93 22.490366624590592 26.79687656533025 27.54921558170496 23.163541228374196 94-95 22.41792008075734 26.771117485643448 27.56573150316332 23.24523093043589 96-97 22.506336733101833 26.661340940539148 27.539275809238973 23.29304651712005 98-99 22.529673922541953 26.620572356628436 27.322430693354192 23.52732302747542 100-101 22.66547445728333 26.235922780291382 27.803936677656814 23.294666084768473 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1313.0 1 756.5 2 217.5 3 252.0 4 222.0 5 203.0 6 317.5 7 570.0 8 861.5 9 993.5 10 993.0 11 965.5 12 1009.5 13 1123.0 14 1288.0 15 1411.5 16 1461.5 17 1522.0 18 1572.0 19 1663.5 20 1781.0 21 1996.0 22 2348.5 23 2841.0 24 3464.5 25 4375.5 26 5554.5 27 6986.0 28 8487.0 29 10194.5 30 12383.5 31 14744.5 32 17265.5 33 19633.5 34 21873.0 35 24180.5 36 27015.0 37 29728.0 38 31627.5 39 33230.5 40 34992.0 41 37655.5 42 40045.5 43 41707.5 44 43259.5 45 44631.0 46 45007.0 47 45564.0 48 48086.0 49 48376.5 50 45778.0 51 43681.5 52 41836.0 53 40794.0 54 39821.0 55 36944.5 56 32727.5 57 29053.0 58 26038.0 59 22213.5 60 18592.0 61 15821.5 62 13249.0 63 10556.0 64 8046.5 65 6132.0 66 4684.0 67 3807.5 68 2967.5 69 2074.5 70 1547.0 71 1196.0 72 961.5 73 784.5 74 578.0 75 490.0 76 379.5 77 203.0 78 117.0 79 88.0 80 53.5 81 45.5 82 38.0 83 25.0 84 19.5 85 12.0 86 7.5 87 6.0 88 8.5 89 8.0 90 5.0 91 4.5 92 3.0 93 2.0 94 2.5 95 1.5 96 1.0 97 1.5 98 2.5 99 2.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2892645434134404 2 0.17937965525843738 3 0.1785311233035725 4 0.2021203116488164 5 0.2158665293176276 6 0.1070847327039489 7 0.1236311058238142 8 0.13703791071067944 9 0.13941380018430116 10-11 0.12660096766584134 12-13 0.11697012997812484 14-15 0.13131032001534146 16-17 0.1079332646588138 18-19 0.13568025958289565 20-21 0.16291813533405855 22-23 0.16648196954449107 24-25 0.19045299726942416 26-27 0.17008823035266685 28-29 0.16834873984519383 30-31 0.15774209040938272 32-33 0.1677547674767884 34-35 0.3482799408742934 36-37 0.4664804421869723 38-39 0.43376953532693086 40-41 0.42053243683103864 42-43 0.4459883954769853 44-45 0.4515038531836071 46-47 0.4541343022436882 48-49 0.4669895613598912 50-51 0.4594376269615937 52-53 0.46435911229981014 54-55 0.4477278859844583 56-57 0.40992578739522756 58-59 0.39970097733910565 60-61 0.3542196645583476 62-63 0.4626196217923371 64-65 0.46130439726229644 66-67 0.47080795515678325 68-69 0.4819237237655133 70-71 0.4742869361717293 72-73 0.46788051991249935 74-75 0.4714867807206751 76-77 0.4474308998002555 78-79 0.4328361501765795 80-81 0.43050268730070107 82-83 0.44395191878530954 84-85 0.44624295506344475 86-87 0.4464126614544177 88-89 0.4546009948188639 90-91 0.47246259246876976 92-93 0.4777659171866754 94-95 0.4759840000814591 96-97 0.47373539040106705 98-99 0.47080795515678325 100-101 0.45260694472493135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.25432128233983 #Duplication Level Percentage of deduplicated Percentage of total 1 82.43979955099135 56.26872565005059 2 10.952218208024615 14.950724406496086 3 3.044784040133006 6.234590043317366 4 1.2855405221431269 3.5097478327929554 5 0.6371559738192877 2.1744324272011877 6 0.37989710354104494 1.555777137559248 7 0.24859834273837095 1.1877537808865402 8 0.17012361375931856 0.9289337432992973 9 0.13250758750634523 0.8139793905005263 >10 0.6539320641598999 7.897532000786514 >50 0.03864333312343687 1.8378847843131527 >100 0.015804130269626793 1.7919126598306043 >500 6.222061191230816E-4 0.24665563292996254 >1k 3.73323671473849E-4 0.601350510035859 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3204 0.2718696383387102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2167 0.18387687461922128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1709 0.1450141110864094 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.242659774324441E-5 0.0 18-19 0.0 0.0 0.0 8.485319548648882E-5 0.0 20-21 0.0 0.0 0.0 8.485319548648882E-5 0.0 22-23 0.0 0.0 0.0 8.485319548648882E-5 0.0 24-25 0.0 0.0 0.0 3.394127819459553E-4 0.0 26-27 0.0 0.0 0.0 5.939723684054217E-4 0.0 28-29 0.0 0.0 0.0 0.0019516234961892429 0.0 30-31 0.0 0.0 0.0 0.006067003477283952 0.0 32-33 0.0 0.0 0.0 0.014340190037216611 0.0 34-35 0.0 0.0 0.0 0.026601476785014247 0.0 36-37 0.0 0.0 0.0 0.04815418843858241 0.0 38-39 0.0 0.0 0.0 0.08413194332485367 0.0 40-41 0.0 0.0 0.0 0.12286742706443582 0.0 42-43 0.0 0.0 0.0 0.16414850666861264 0.0 44-45 0.0 0.0 0.0 0.21213298871622205 0.0 46-47 0.0 0.0 0.0 0.2719544915341967 0.0 48-49 0.0 0.0 0.0 0.3384793967956039 0.0 50-51 0.0 0.0 0.0 0.41518668551538984 0.0 52-53 0.0 0.0 0.0 0.48973021775027026 0.0 54-55 0.0 0.0 0.0 0.5758137845713132 0.0 56-57 0.0 0.0 0.0 0.6775527659596132 0.0 58-59 0.0 0.0 0.0 0.7910863415205354 0.0 60-61 0.0 0.0 0.0 0.9205723178329173 0.0 62-63 0.0 0.0 0.0 1.0581193477165156 0.0 64-65 0.0 0.0 0.0 1.2014363948931952 0.0 66-67 0.0 0.0 0.0 1.3655000483663216 0.0 68-69 0.0 0.0 0.0 1.55391656894407 0.0 70-71 0.0 0.0 0.0 1.7701225110436434 0.0 72-73 0.0 0.0 0.0 1.995195612071555 0.0 74-75 0.0 0.0 0.0 2.2189110619716828 0.0 76-77 0.0 0.0 0.0 2.4655368746531625 0.0 78-79 0.0 0.0 0.0 2.7604441555664545 0.0 80-81 0.0 0.0 0.0 3.0744434054642067 0.0 82-83 0.0 0.0 0.0 3.4125833894778643 0.0 84-85 0.0 0.0 0.0 3.757299496141725 0.0 86-87 0.0 0.0 0.0 4.109991803181316 0.0 88-89 0.0 0.0 0.0 4.50443188240026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4240 0.0 37.94666 1 GGTATCA 2480 0.0 34.256348 1 ATCAACG 5120 0.0 30.930807 3 TCAACGC 5180 0.0 30.662764 4 CAACGCA 5275 0.0 30.110544 5 TATCAAC 5320 0.0 29.767992 2 AACGCAG 5450 0.0 29.040644 6 GTACATG 4870 0.0 27.67764 1 TACATGG 4825 0.0 27.318834 2 GTGTACG 90 8.990335E-4 26.367393 1 ACATGGG 4970 0.0 25.376982 3 ACGCAGA 6195 0.0 25.012823 7 CGCAGAG 6320 0.0 24.443129 8 CATGGGG 2630 0.0 22.714912 4 GCAGAGT 6920 0.0 21.844437 9 GTACTAG 225 7.21393E-8 21.093914 1 GTACTTT 3250 0.0 20.779884 14-15 GAGTACT 3290 0.0 20.453043 12-13 CATGGGA 2615 0.0 20.30685 4 AGAGTAC 6015 0.0 19.103542 10-11 >>END_MODULE