FastQCFastQC Report
Fri 27 May 2016
SRR939255_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939255_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178506
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33830.2870583603307917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24450.20746606296446518No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19170.16266357574759907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.038.2199481
TAGCGCG500.001623058737.96554
GTATCAA44350.034.2407531
ATCAACG54450.027.4543913
TCAACGC54850.027.3406964
CAACGCA55550.026.996175
TATCAAC55850.026.8511582
AACGCAG57350.026.0661136
GTACATG47000.024.2072981
TACATGG47800.023.0334632
GTAGCGC1252.2698667E-422.7792993
ACGCAGA65100.022.671417
CGCAGAG65900.022.3961898
TACCTGG10350.022.0089842
ACATGGG48850.021.566893
CATGGGG24500.020.5323624
GCAGAGT72500.019.7682429
GTACCTG12650.019.5685711
GAGTACT35300.019.22555512-13
AGAGTAC61150.018.6261510-11
Produced by FastQC (version 0.11.5)