##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939255_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1178506 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.234228760820905 33.0 31.0 34.0 31.0 34.0 2 32.453164430219275 34.0 31.0 34.0 31.0 34.0 3 32.54760264266792 34.0 31.0 34.0 31.0 34.0 4 36.09261811140546 37.0 35.0 37.0 35.0 37.0 5 36.03115300219091 37.0 35.0 37.0 35.0 37.0 6 36.057433733896985 37.0 35.0 37.0 35.0 37.0 7 36.001463717622144 37.0 35.0 37.0 35.0 37.0 8 35.966569538042236 37.0 35.0 37.0 35.0 37.0 9 37.66924309252562 39.0 37.0 39.0 35.0 39.0 10-11 37.57868351964267 39.0 37.0 39.0 35.0 39.0 12-13 37.49886933117014 39.0 37.0 39.0 35.0 39.0 14-15 38.77123833056429 40.0 38.0 41.0 34.5 41.0 16-17 38.771847152241904 40.0 38.0 41.0 34.5 41.0 18-19 38.875660369993874 40.0 38.0 41.0 35.0 41.0 20-21 38.85128416826049 40.0 38.0 41.0 34.5 41.0 22-23 38.766007979594505 40.0 38.0 41.0 34.0 41.0 24-25 38.69783395247882 40.0 38.0 41.0 34.0 41.0 26-27 38.53029089372477 40.0 38.0 41.0 34.0 41.0 28-29 38.35988785801685 40.0 38.0 41.0 34.0 41.0 30-31 38.164513799675184 40.0 38.0 41.0 33.5 41.0 32-33 38.11496801883062 40.0 38.0 41.0 33.0 41.0 34-35 38.02385011192136 40.0 37.5 41.0 33.0 41.0 36-37 37.87059463422333 40.0 37.0 41.0 33.0 41.0 38-39 37.738815924568904 40.0 37.0 41.0 33.0 41.0 40-41 37.54266291389267 40.0 37.0 41.0 32.5 41.0 42-43 37.362064766747054 40.0 37.0 41.0 32.0 41.0 44-45 37.109084722521565 40.0 36.0 41.0 31.0 41.0 46-47 36.80787454624753 40.0 36.0 41.0 31.0 41.0 48-49 36.49836742451884 39.0 36.0 41.0 30.0 41.0 50-51 36.24762962598409 39.0 35.0 40.5 30.0 41.0 52-53 35.972983166823084 39.0 35.0 40.0 29.0 41.0 54-55 35.71225263172185 38.5 35.0 40.0 28.0 41.0 56-57 35.381541969239024 38.0 34.5 40.0 28.0 41.0 58-59 35.05069214751558 38.0 34.0 40.0 27.0 41.0 60-61 34.7008610902278 38.0 34.0 40.0 26.5 41.0 62-63 34.32181889612781 37.0 34.0 40.0 26.0 41.0 64-65 33.986078136216534 36.5 33.0 39.0 26.0 41.0 66-67 33.54348344429303 36.0 33.0 39.0 25.0 40.0 68-69 33.05507651212637 35.5 32.0 39.0 23.5 40.0 70-71 32.551990825672505 35.0 31.5 38.0 22.0 40.0 72-73 32.027911610123326 35.0 31.0 37.5 21.5 39.5 74-75 31.469009915944426 35.0 31.0 37.0 19.5 39.0 76-77 29.53680380074433 32.5 28.5 35.0 17.0 37.0 78-79 30.508296096922713 34.0 30.0 36.0 18.0 38.0 80-81 30.49472510110258 34.0 30.0 35.5 17.5 37.0 82-83 30.10355823389953 34.0 30.0 35.0 12.0 37.0 84-85 29.599123805903407 34.0 29.5 35.0 7.0 36.0 86-87 28.92918406864284 34.0 29.0 35.0 2.0 36.0 88-89 28.283531861526374 33.0 27.5 35.0 2.0 35.5 90-91 27.73032169543473 33.0 26.5 35.0 2.0 35.0 92-93 27.28668670333456 33.0 26.0 35.0 2.0 35.0 94-95 26.660613098278667 32.0 25.0 34.0 2.0 35.0 96-97 25.810391716291644 32.0 24.0 34.0 2.0 35.0 98-99 24.652346699974373 32.0 11.0 34.0 2.0 35.0 100-101 20.261042370594634 27.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1532611541986526 1101 2 0.24972736337053902 1101 3 0.25037827210068997 1101 4 0.014964098320390917 1101 5 0.13397676259078395 1101 6 0.18626217578363935 1101 7 0.22105565242672753 1101 8 0.21847326032587944 1101 9 0.27542854302564024 1101 10-11 0.32388576095286226 1101 12-13 0.3049782012529292 1101 14-15 0.2864573665721508 1101 16-17 0.3247841928634685 1101 18-19 0.23867731256870428 1101 20-21 0.2839294475212668 1101 22-23 0.3862648486033393 1101 24-25 0.3538084871932341 1101 26-27 0.33213675981810553 1101 28-29 0.42643438844923054 1101 30-31 0.4268236108609855 1101 32-33 0.676587386925938 1101 34-35 0.6205206063178466 1101 36-37 0.6162150150501091 1101 38-39 0.8410559665596509 1101 40-41 0.7111889472966126 1101 42-43 0.7141868886598957 1101 44-45 0.684149595708881 1101 46-47 0.7913995858038163 1101 48-49 0.7768300522144713 1101 50-51 0.8918600485824868 1101 52-53 0.8267389145479527 1101 54-55 0.7305667432047258 1101 56-57 0.9218854255822322 1101 58-59 0.857467861067164 1101 60-61 0.7925290044877968 1101 62-63 0.8973191136311272 1101 64-65 0.959504682352339 1101 66-67 0.9885725285988869 1101 68-69 1.138463356361946 1101 70-71 0.8111005360296772 1101 72-73 0.9170388028107297 1101 74-75 0.9689479344418501 1101 76-77 0.7793440974685737 1101 78-79 1.0813969270217818 1101 80-81 1.1169273293050956 1101 82-83 0.9721068097128587 1101 84-85 0.6502260044742627 1101 86-87 0.8086933051410021 1101 88-89 0.9503729781736006 1101 90-91 1.1402123874743388 1101 92-93 1.1734092746909894 1101 94-95 1.1115219901757847 1101 96-97 1.2739962640220064 1101 98-99 0.5833004167063542 1101 100-101 0.15696468922338624 1102 1 0.2493760495394568 1102 2 0.20566973868669436 1102 3 0.16270215223536155 1102 4 0.07349427400622943 1102 5 0.17385584879292537 1102 6 0.1742933437659957 1102 7 0.21098290670994402 1102 8 0.24445508921515113 1102 9 0.3437012662753389 1102 10-11 0.2371806619444783 1102 12-13 0.25822537669707657 1102 14-15 0.3101227022338904 1102 16-17 0.24796376278888488 1102 18-19 0.30312807730081914 1102 20-21 0.2962914536282284 1102 22-23 0.3018719876916478 1102 24-25 0.3982414144168871 1102 26-27 0.37177160151021127 1102 28-29 0.2872745895637365 1102 30-31 0.3380683898154473 1102 32-33 0.45494357882212455 1102 34-35 0.3248392237066966 1102 36-37 0.4279978127475772 1102 38-39 0.5401084340249227 1102 40-41 0.6135932664246226 1102 42-43 0.6904202808842399 1102 44-45 0.6659980585854228 1102 46-47 0.5897889336794861 1102 48-49 0.5340961136313283 1102 50-51 0.34801858454515155 1102 52-53 0.3767698919004445 1102 54-55 0.14890397423790347 1102 56-57 0.4023969263498586 1102 58-59 0.38087278272169556 1102 60-61 0.4182791840543203 1102 62-63 0.3840916139119699 1102 64-65 0.4178358881202513 1102 66-67 0.22752450689431925 1102 68-69 0.2865665892401452 1102 70-71 0.21362995600428292 1102 72-73 0.29757855764710683 1102 74-75 0.30670234871458035 1102 76-77 0.20441294548551525 1102 78-79 0.5548185287891911 1102 80-81 0.7422809306776301 1102 82-83 0.47741210684013424 1102 84-85 0.5305819376584928 1102 86-87 0.6209160833669891 1102 88-89 0.5337692874433166 1102 90-91 0.5490615277677087 1102 92-93 0.26100880220126044 1102 94-95 0.35591883388993395 1102 96-97 0.28690207584991256 1102 98-99 -0.13274023511160848 1102 100-101 -0.323589362852605 1103 1 0.0686024333386186 1103 2 0.14341804827549254 1103 3 0.2531194563535095 1103 4 0.12761792858123044 1103 5 0.18929954043752417 1103 6 0.1703949361663888 1103 7 0.09026103208584857 1103 8 0.1706538792009411 1103 9 0.24247566520729436 1103 10-11 0.13384236453715204 1103 12-13 0.08456241529656694 1103 14-15 0.1462786114303114 1103 16-17 0.24333641565440445 1103 18-19 0.24499461995035432 1103 20-21 0.21841097768704998 1103 22-23 0.20290393904711124 1103 24-25 0.27670885398818257 1103 26-27 0.14324926910700952 1103 28-29 0.1979068567224047 1103 30-31 0.43051706755894514 1103 32-33 0.40887214359482016 1103 34-35 0.24953396704034958 1103 36-37 0.038588656148832 1103 38-39 0.007650929378506532 1103 40-41 -0.09583601754582816 1103 42-43 -0.07871851157511145 1103 44-45 0.03694102933804544 1103 46-47 0.21498609890521436 1103 48-49 0.0023256164954190695 1103 50-51 -0.03846634279808825 1103 52-53 -0.1364238713250856 1103 54-55 -0.29391090985181023 1103 56-57 0.05992071747924399 1103 58-59 -0.07627438258874264 1103 60-61 -0.01834416132493999 1103 62-63 0.0435184412587617 1103 64-65 0.11710322129432171 1103 66-67 -0.08916223631734965 1103 68-69 -0.28133872401325277 1103 70-71 -0.3362695765595376 1103 72-73 -0.19627056499940565 1103 74-75 -0.18479942767844904 1103 76-77 0.1747267664501706 1103 78-79 0.0014013652915707553 1103 80-81 0.045209310779991796 1103 82-83 -0.018047798520555602 1103 84-85 -0.03989986553714786 1103 86-87 -0.1321140348389669 1103 88-89 0.09925567351273301 1103 90-91 0.08054146562360032 1103 92-93 0.1064050047693641 1103 94-95 -0.2314920711471622 1103 96-97 -0.30843737024075324 1103 98-99 -0.6373989253754146 1103 100-101 -0.8484190510647096 1104 1 0.23341421826010844 1104 2 0.18985983691205632 1104 3 0.22206377971092905 1104 4 0.20308090823888847 1104 5 0.12887883967211877 1104 6 0.18558481170719432 1104 7 0.1950563297908019 1104 8 0.24952248380041908 1104 9 0.2540435525373823 1104 10-11 0.22248966641171108 1104 12-13 0.2814815029815563 1104 14-15 0.24441277175213116 1104 16-17 0.33152876763215744 1104 18-19 0.20823131381896332 1104 20-21 0.30384218213440306 1104 22-23 0.33618287407020375 1104 24-25 0.4769425766356292 1104 26-27 0.4607036630005368 1104 28-29 0.3801916528419582 1104 30-31 0.35576104230315053 1104 32-33 0.23718956695987004 1104 34-35 0.28890034133706166 1104 36-37 0.5194606336671796 1104 38-39 0.37092485785619544 1104 40-41 0.5058941338497576 1104 42-43 0.4884216104772534 1104 44-45 0.37553780451899144 1104 46-47 0.6376307792620324 1104 48-49 0.5091372856877214 1104 50-51 0.5278724804037722 1104 52-53 0.5255777069527099 1104 54-55 0.6928988976100285 1104 56-57 0.6079684582861091 1104 58-59 0.6674647101447206 1104 60-61 0.7804438689223119 1104 62-63 0.7430401778004239 1104 64-65 0.8049275041297577 1104 66-67 0.7041668144768565 1104 68-69 0.9976478740433308 1104 70-71 0.8005500007941109 1104 72-73 0.8327162427115127 1104 74-75 0.8920380457688282 1104 76-77 0.6064570268660034 1104 78-79 0.9315650885512099 1104 80-81 0.8823125085117027 1104 82-83 0.8108541411174457 1104 84-85 0.7691863854157326 1104 86-87 0.8593106837420272 1104 88-89 0.9936787844643256 1104 90-91 0.8693948157740046 1104 92-93 0.8443291249968965 1104 94-95 0.6574938644017401 1104 96-97 0.8935805107577721 1104 98-99 1.0074567024596384 1104 100-101 0.6765122032803781 1105 1 0.026474353040455867 1105 2 0.05328643199366212 1105 3 0.11616403100840245 1105 4 0.06576778746958212 1105 5 0.07409393145945131 1105 6 0.08702836639825051 1105 7 0.1105449818051838 1105 8 0.10073267952416387 1105 9 0.15856239271995065 1105 10-11 0.07352522900362857 1105 12-13 0.09779397986542904 1105 14-15 0.14339443487730108 1105 16-17 0.20934933030724068 1105 18-19 0.022572535078751343 1105 20-21 0.17192566073134685 1105 22-23 0.20839894079352206 1105 24-25 0.10164449253136354 1105 26-27 0.06486856419619613 1105 28-29 0.18972709703182744 1105 30-31 0.1425526000386057 1105 32-33 0.11725699673240086 1105 34-35 0.3503015088842929 1105 36-37 0.1263436342207811 1105 38-39 0.09720227347592214 1105 40-41 0.20375307621448968 1105 42-43 0.20127920250416054 1105 44-45 0.4140220364539644 1105 46-47 0.3359276904335573 1105 48-49 0.3931365348840501 1105 50-51 0.48216928703301676 1105 52-53 0.44957150727854156 1105 54-55 0.5541293127616953 1105 56-57 0.6025215677458391 1105 58-59 0.5049101719404518 1105 60-61 0.3160876395090142 1105 62-63 0.5290112370117512 1105 64-65 0.5470569353959789 1105 66-67 0.4151858087008975 1105 68-69 0.527791690961287 1105 70-71 0.6665826290742203 1105 72-73 0.6030435788553419 1105 74-75 0.5212479516253339 1105 76-77 0.6236336407572978 1105 78-79 0.6154704080902391 1105 80-81 0.7465421784036046 1105 82-83 0.8833898537909022 1105 84-85 0.8699461385375464 1105 86-87 0.8617127802899915 1105 88-89 0.7613458363006629 1105 90-91 0.9111075826835204 1105 92-93 0.653683600158999 1105 94-95 0.7199349108699948 1105 96-97 0.4131326896327856 1105 98-99 0.541751719198281 1105 100-101 0.07011904668157953 1106 1 0.10644843650528912 1106 2 0.14522511884980815 1106 3 0.2303253341047906 1106 4 0.14230135484962148 1106 5 0.030876557628943146 1106 6 0.14845897687006726 1106 7 0.1351147141290454 1106 8 0.03444456566871423 1106 9 0.08188106858300159 1106 10-11 0.17843437337379697 1106 12-13 0.1621815724350455 1106 14-15 0.29535450213617054 1106 16-17 0.2159115772737934 1106 18-19 0.3305973523036414 1106 20-21 0.2737175069224733 1106 22-23 0.3596575328489351 1106 24-25 0.2026373440594611 1106 26-27 0.25796226562116686 1106 28-29 0.37158980736356995 1106 30-31 0.3999341582222584 1106 32-33 0.2813309017070793 1106 34-35 0.3153891729681675 1106 36-37 0.29415615453733324 1106 38-39 0.42971925072729533 1106 40-41 0.4137915225226365 1106 42-43 0.32277736736524076 1106 44-45 0.5957181510422913 1106 46-47 0.5545327041158359 1106 48-49 0.3762664071631079 1106 50-51 0.25633453523481364 1106 52-53 0.4556424630283473 1106 54-55 0.28531544490352445 1106 56-57 0.45346196562523744 1106 58-59 0.4389449065988771 1106 60-61 0.500879627546631 1106 62-63 0.6279066813754142 1106 64-65 0.43214133405552957 1106 66-67 0.47330334329075896 1106 68-69 0.5164332596652272 1106 70-71 0.6054911203595239 1106 72-73 0.435538023201282 1106 74-75 0.40098909656289194 1106 76-77 0.44403973542800657 1106 78-79 0.49758969803036734 1106 80-81 0.5318076529687552 1106 82-83 0.5902160682036879 1106 84-85 0.8126951973270025 1106 86-87 0.7731809948564425 1106 88-89 0.8773348904801104 1106 90-91 0.9578049975313014 1106 92-93 0.987293238627764 1106 94-95 1.47249294972411 1106 96-97 1.385917372576337 1106 98-99 1.3364267134357668 1106 100-101 0.8170667614755764 1107 1 0.027087717858861993 1107 2 0.0714155950402855 1107 3 0.017251899602783283 1107 4 0.015308506428198143 1107 5 0.04661875194256737 1107 6 0.04014308641131947 1107 7 0.028185532199863417 1107 8 0.04732612855028151 1107 9 0.06019085403563906 1107 10-11 0.1452035169286603 1107 12-13 0.040005137758612364 1107 14-15 0.1688609559997687 1107 16-17 0.12060153017802833 1107 18-19 0.23043600571372025 1107 20-21 0.22055598590380754 1107 22-23 0.13385952133663892 1107 24-25 0.030809766874739353 1107 26-27 -0.026020814790491897 1107 28-29 0.13899343548147414 1107 30-31 0.13063579785247015 1107 32-33 0.14452425597186647 1107 34-35 0.19623035559506263 1107 36-37 0.2180223676892652 1107 38-39 0.1566641312435948 1107 40-41 0.18276500935326823 1107 42-43 0.1203135613300006 1107 44-45 0.31861410429538495 1107 46-47 0.1701331750734525 1107 48-49 0.16623239355766373 1107 50-51 0.34403076757391204 1107 52-53 0.3727561033562736 1107 54-55 0.3552788148668853 1107 56-57 0.4568262600609643 1107 58-59 0.4730152013834541 1107 60-61 0.36169420978978195 1107 62-63 0.33148029030616755 1107 64-65 0.16800824492244004 1107 66-67 0.3326243793975081 1107 68-69 0.6311833904867825 1107 70-71 0.47967566597264266 1107 72-73 0.589992497298617 1107 74-75 0.3421346573513091 1107 76-77 0.37741667594780637 1107 78-79 0.5809600974722002 1107 80-81 0.32201129852954224 1107 82-83 0.5394180566913889 1107 84-85 0.5642977614295681 1107 86-87 0.6690579353762409 1107 88-89 0.8969581243890623 1107 90-91 0.8190360284051899 1107 92-93 0.5677921048409154 1107 94-95 0.777871896174485 1107 96-97 0.7948045171777665 1107 98-99 0.8301865250527207 1107 100-101 1.0211549352066065 1108 1 0.06573098673468536 1108 2 0.10535800921525862 1108 3 0.09747451936028995 1108 4 0.10441178041519095 1108 5 0.09570016148909133 1108 6 0.060478435816435194 1108 7 0.09261748267832104 1108 8 0.026692627684219872 1108 9 0.10798453322466628 1108 10-11 0.05255437020974085 1108 12-13 0.14522420809395697 1108 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sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 3.0 5 25.0 6 66.0 7 154.0 8 344.0 9 642.0 10 1049.0 11 1693.0 12 2413.0 13 3356.0 14 4252.0 15 5668.0 16 6948.0 17 9588.0 18 9261.0 19 6956.0 20 6983.0 21 7472.0 22 8124.0 23 9160.0 24 10402.0 25 12131.0 26 14860.0 27 17808.0 28 21354.0 29 26055.0 30 31625.0 31 38338.0 32 47222.0 33 60753.0 34 81317.0 35 115911.0 36 177402.0 37 259735.0 38 171076.0 39 8355.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.12817741619791 19.64283586789097 15.126756248457566 23.10223046745356 2 15.414855758052992 20.652928368629432 40.209723157964405 23.722492715353166 3 19.189804718855907 24.67921249446333 29.822164672899415 26.308818113781346 4 13.232601276531472 17.095203588271932 36.87770787760096 32.794487257595634 5 13.906165942303222 37.47329245672063 33.99999660587218 14.620544995103971 6 31.992370000661857 36.3982873231023 17.407633054053182 14.201709622182662 7 28.777452130069765 32.46551141869452 20.629169473893217 18.1278669773425 8 26.02897227506691 36.04377067235975 19.553570367906485 18.373686684666858 9 26.362360480133322 17.005428907447225 20.100534066012393 36.53167654640706 10-11 24.962452460997227 26.81267638858012 27.239997081050078 20.984874069372577 12-13 25.720700615864494 23.928813260178565 27.950557740053934 22.39992838390301 14-15 22.57061045464516 25.4015199952821 26.104537966152463 25.923331583920277 16-17 22.208650332881348 28.68040505796361 26.66966766171856 22.441276947436485 18-19 22.215042696024753 27.160331453692834 28.98883373900249 21.635792111279923 20-21 22.966917436143728 26.445856024492027 28.86905115459743 21.718175384766816 22-23 22.556143116793635 26.342716965378198 28.770112328660186 22.33102758916798 24-25 22.406546933150956 26.572202432571405 28.85623832207897 22.165012312198666 26-27 22.341568342942967 26.713293537150882 28.743407961782086 22.201730158124064 28-29 22.13081647441761 26.877716362920513 28.905198615874678 22.086268546787203 30-31 22.370654031460173 26.65425547260684 28.762390687870916 22.212699808062073 32-33 22.189747018682976 26.72629583557487 28.71139391738354 22.372563228358615 34-35 22.602704563765734 26.817506647280272 28.460245084915904 22.11954370403809 36-37 22.643409148960185 26.599269026814515 28.49047333356528 22.266848490660024 38-39 22.385271511220406 26.697952783166084 28.675461060791967 22.241314644821543 40-41 22.52670416167431 26.631482568894533 28.57243472033182 22.26937854909934 42-43 22.530626791203243 26.626248141179165 28.30235828943935 22.540766778178238 44-45 22.476945112907003 26.890254664435563 28.233157289145787 22.39964293351164 46-47 22.576268367581584 27.034636314035687 27.89454078606503 22.494554532317697 48-49 22.68069011308843 26.9320175392076 27.855477608235873 22.531814739468096 50-51 22.648463393482935 27.189763989322074 27.648607643915263 22.51316497327973 52-53 22.792073679851743 27.113739113316544 27.52621119656785 22.56797601026386 54-55 22.55593098380492 27.07075738265227 27.60002918949925 22.77328244404356 56-57 22.55462786694742 27.29583838390695 27.551672830593898 22.59786091855173 58-59 22.593881127040223 26.993343148194505 27.74536799365287 22.6674077311124 60-61 22.61211082911398 27.062517660176205 27.789501730591198 22.53586978011861 62-63 22.486660919208614 27.07141838892415 27.78113894706116 22.660781744806073 64-65 22.369961251738964 26.84516690432663 27.84851479141573 22.93635705251868 66-67 22.340903961130316 27.126385869519105 27.610093152154175 22.922617017196405 68-69 22.386151741108836 26.9312147986296 27.76533480414514 22.91729865611642 70-71 22.419741605049104 27.011444999007217 27.591883282732542 22.976930113211132 72-73 22.45898620796161 26.89116559440512 27.539444007921894 23.11040418971138 74-75 22.328439566705644 27.189806415919815 27.3161528239992 23.16560119337534 76-77 22.3615340541005 27.015114501933496 27.447072870589867 23.176278573376134 78-79 22.41047870415429 27.090774791271482 27.389925112717005 23.108821391857223 80-81 22.400381161039267 27.262178464316428 27.3632811754219 22.974159199222406 82-83 22.470992935122943 27.076824386129555 27.325401822307228 23.126780856440273 84-85 22.40790458427874 26.99265001620696 27.270459378229724 23.328986021284575 86-87 22.356242949754684 26.899956169559246 27.453188069965616 23.29061281072045 88-89 22.272138416580145 26.845169703231214 27.40117998874011 23.48151189144853 90-91 22.264811875451215 26.971275807805423 27.440518302371697 23.32339401437167 92-93 22.300225879206383 26.789426613016822 27.441947686307916 23.46839982146888 94-95 22.14596276981195 26.72735650051845 27.43855355848846 23.68812717118114 96-97 22.200353668118787 26.663843883696813 27.417849378789754 23.71795306939464 98-99 22.139659992957178 26.693522725826362 27.179816801795496 23.98700047942096 100-101 22.26768021539254 26.085798084767966 27.888471738850974 23.75804996098853 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 124.0 2 108.0 3 141.0 4 126.0 5 131.5 6 222.5 7 483.0 8 761.5 9 853.0 10 809.5 11 752.0 12 813.5 13 946.5 14 1063.5 15 1175.5 16 1230.5 17 1306.0 18 1449.5 19 1547.0 20 1700.0 21 1975.5 22 2298.0 23 2846.0 24 3582.5 25 4486.0 26 5683.0 27 7166.5 28 8646.0 29 10440.0 30 12605.5 31 14835.5 32 17457.5 33 19789.0 34 22004.5 35 24358.0 36 27028.5 37 29767.0 38 31684.5 39 33196.0 40 35356.5 41 37880.0 42 40174.5 43 41877.5 44 43048.5 45 44751.5 46 45475.5 47 45734.0 48 47937.0 49 48329.5 50 45959.0 51 44016.0 52 42461.5 53 41182.5 54 39892.0 55 37277.0 56 33010.0 57 29118.5 58 26000.5 59 22377.5 60 18731.5 61 15743.5 62 13145.0 63 10353.5 64 7890.5 65 6034.5 66 4529.0 67 3657.5 68 2813.0 69 1947.5 70 1438.5 71 1099.0 72 911.5 73 749.0 74 586.5 75 499.0 76 349.5 77 190.5 78 109.0 79 69.5 80 44.5 81 26.5 82 16.5 83 15.0 84 9.5 85 3.0 86 2.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28985851578184585 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013152245300405768 16-17 6.788255638919106E-4 18-19 4.242659774324441E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 8.485319548648882E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0014000777255270657 36-37 0.006533696052459639 38-39 0.002375889473621687 40-41 0.0032244214284865755 42-43 2.9698618420271087E-4 44-45 0.0011879447368108435 46-47 2.5455958645946645E-4 48-49 2.9698618420271087E-4 50-51 0.0 52-53 1.6970639097297764E-4 54-55 0.0 56-57 8.061053571216439E-4 58-59 0.0017819171052162654 60-61 5.939723684054217E-4 62-63 0.0011879447368108435 64-65 1.2727979322973323E-4 66-67 3.394127819459553E-4 68-69 0.0015697841165000432 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.2584204068541017 78-79 1.2727979322973323E-4 80-81 1.2727979322973323E-4 82-83 0.0 84-85 0.0 86-87 0.10538766879421912 88-89 0.37564509641868604 90-91 0.2721666245229129 92-93 0.0 94-95 0.0 96-97 0.0 98-99 8.485319548648882E-5 100-101 5.515457706621774E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1178506.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.07554753473543 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57738325795557 54.718476484783295 2 11.469883195799477 15.386973910354222 3 3.2176544217324077 6.474777963457915 4 1.3252389975433507 3.555645254984166 5 0.6693261537475865 2.244770912096894 6 0.3964307131272393 1.5954484285557125 7 0.2670302359021627 1.253783949702699 8 0.18445551144398073 0.9897963540723732 9 0.14191612893690533 0.8567191847207735 >10 0.6910856670420292 8.148647964023665 >50 0.040852546283776964 1.8817736200648152 >100 0.017856673490037236 2.018835745977847 >500 5.065697116518551E-4 0.216442667727585 >1k 3.799272837388913E-4 0.6579075594780792 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3383 0.2870583603307917 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2445 0.20746606296446518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1917 0.16266357574759907 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.242659774324441E-5 0.0 18-19 0.0 0.0 0.0 8.485319548648882E-5 0.0 20-21 0.0 0.0 0.0 8.485319548648882E-5 0.0 22-23 0.0 0.0 0.0 8.485319548648882E-5 0.0 24-25 0.0 0.0 0.0 2.9698618420271087E-4 0.0 26-27 0.0 0.0 0.0 5.091191729189329E-4 0.0 28-29 0.0 0.0 0.0 0.0019091968984459985 0.0 30-31 0.0 0.0 0.0 0.006109430075027195 0.0 32-33 0.0 0.0 0.0 0.014594749623676078 0.0 34-35 0.0 0.0 0.0 0.026771183175987225 0.0 36-37 0.0 0.0 0.0 0.048111761840839165 0.0 38-39 0.0 0.0 0.0 0.08442892950905638 0.0 40-41 0.0 0.0 0.0 0.12337654623735475 0.0 42-43 0.0 0.0 0.0 0.16491218542799102 0.0 44-45 0.0 0.0 0.0 0.2133209334530329 0.0 46-47 0.0 0.0 0.0 0.2732697160642372 0.0 48-49 0.0 0.0 0.0 0.34038859369404995 0.0 50-51 0.0 0.0 0.0 0.4172655888048088 0.0 52-53 0.0 0.0 0.0 0.49231824021260817 0.0 54-55 0.0 0.0 0.0 0.5784866602291376 0.0 56-57 0.0 0.0 0.0 0.6809044671813296 0.0 58-59 0.0 0.0 0.0 0.7948623087196841 0.0 60-61 0.0 0.0 0.0 0.9240088722501201 0.0 62-63 0.0 0.0 0.0 1.0615134755359752 0.0 64-65 0.0 0.0 0.0 1.2055093482765469 0.0 66-67 0.0 0.0 0.0 1.369742708140646 0.0 68-69 0.0 0.0 0.0 1.5583713617071107 0.0 70-71 0.0 0.0 0.0 1.7747045835999138 0.0 72-73 0.0 0.0 0.0 1.999692831432339 0.0 74-75 0.0 0.0 0.0 2.224044680298616 0.0 76-77 0.0 0.0 0.0 2.470840199371068 0.0 78-79 0.0 0.0 0.0 2.765917186675333 0.0 80-81 0.0 0.0 0.0 3.0814437940918418 0.0 82-83 0.0 0.0 0.0 3.4196686313009863 0.0 84-85 0.0 0.0 0.0 3.7647665773445365 0.0 86-87 0.0 0.0 0.0 4.115507260887938 0.0 88-89 0.0 0.0 0.0 4.506595638885164 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2790 0.0 38.219948 1 TAGCGCG 50 0.0016230587 37.9655 4 GTATCAA 4435 0.0 34.240753 1 ATCAACG 5445 0.0 27.454391 3 TCAACGC 5485 0.0 27.340696 4 CAACGCA 5555 0.0 26.99617 5 TATCAAC 5585 0.0 26.851158 2 AACGCAG 5735 0.0 26.066113 6 GTACATG 4700 0.0 24.207298 1 TACATGG 4780 0.0 23.033463 2 GTAGCGC 125 2.2698667E-4 22.779299 3 ACGCAGA 6510 0.0 22.67141 7 CGCAGAG 6590 0.0 22.396189 8 TACCTGG 1035 0.0 22.008984 2 ACATGGG 4885 0.0 21.56689 3 CATGGGG 2450 0.0 20.532362 4 GCAGAGT 7250 0.0 19.768242 9 GTACCTG 1265 0.0 19.568571 1 GAGTACT 3530 0.0 19.225555 12-13 AGAGTAC 6115 0.0 18.62615 10-11 >>END_MODULE